Structure of the complex bound to cleaved DNA in the presence of Lu3+.

Metal Ion Binding at the Catalytic Site Induces Widely Distributed Changes in a Sequence Specific Protein-DNA Complex.

Backbone assignments and ILV methyl assignments for EcoRV bound to 16-mer double stranded DNA (GCAAAGATATCTTTCG) with Lu3+

Used 2-keto-3,3-d2-1,2,3,4-13C-butyrate and 2-keto-3-methyl-d3-3-d1-1,2,3,4-13C-butyrate as
precursors to achieve {I( 1 only), L(13CH3, 12CD3),V(13CH3,12CD3)} U-[15N,13C,2H] labeling.

Monte Carlo based NMR assignment program

Hitchens, Lukin, Zhan, McCallum and Rule

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax