4D double 15N-edited NOESY (200 ms mix time)

3D 15N-edited NOESY TROSY-HSQC (60 ms mix time)

Structure and multistate function of the transmembrane electron transporter CcdA.

C16 and C118 in the native sequence have been mutated to Ala.

Residues 1-10 represent a non-native sequence left over from protease cleavage
that removed the N-terminal DsbD fusion protein.
The C-terminal HHHHHH is a non-native affinity tag used for purification.

cytochrome C-type biogenesis protein (ccdA) [Archaeoglobus fulgidus DSM 4304]

Cytochrome c biogenesis protein [Archaeoglobus fulgidus DSM 8774]

hypothetical protein [Archaeoglobus fulgidus]

Target of the MPSbyNMR consortium of PSI-Biology
Structure of the Transmembrane Electron Transporter CcdA

3D 15N-edited NOESY TROSY-HSQC (120 ms mix time)

3D 13C-edited Methyl NOESY (150 ms mix time)

Used for collecting triple resonance spectra

simulated annealing, torsion angle dynamics

The sample contains ~100 mM Foscholine-12 (DPC) as membrane mimetic for the membrane protein
Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax