Human Brd4 ET domain in complex with MLV Integrase C-term

Proceedings of the National Academy of Sciences of the United States of America
Structure of the Brd4 ET domain bound to a C-terminal motif from γ-retroviral integrases reveals a conserved mechanism of interaction.

Residues 1-5 (GAIAM) are non-native cloning artifacts.
Residues 6-84 are human Brd4 ET domain (600-678)
Residues 85-86 (TR) are non native cloning artifacts.

residues 1-17 are MLV Integrase C-term EBM(389-405)

Cloned in to SgfI and MluI site to make construct with N-term 6x his-tag and TEV protease site.

Bromodomain-containing Protein 4 (BRD4)
Extraterminal (ET) Domain
Residues 600-678 native sequence

Murine Leukemia Virus (MLV)
Integrase (IN) residues 389-405 of native sequence

3D 13C,15N-filtered(f1) 1H-15N(f2) NOESY

3D 13C,15N-filtered(f1) 1H-13C(f2) NOESY aliphatic

U15N Brd4 ET with MLV IN
water

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax