Nuclear Magnetic Resonance Structure of Ran GTPase Determines C-terminal Tail Conformational Dynamics.

Structure of nucleotide-free human Ran GTPase as solved by NMR
NMR solution structure of nucleotide-free Ran GTPase

Bruker AVIII-600 MHz Avance console. Dexter.

0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.