K-RAS isoleucine C -methyl resonances were first tentatively assigned based on
assignments of T35S H-RAS-GMPPNP (BMRB code: 17610), and then remaining
ambiguous assignments were resolved by mutagenesis of isoleucines 21, 36, 100,
139, and 163 to leucine residues.
The A-RAFRBD isoleucine C -methyl resonance assignments were transferred from BMRB entry: 11265.

Proceedings of the National Academy of Sciences of the United States of America
Oncogenic and RASopathy-associated K-RAS mutations relieve membrane-dependent occlusion of the effector-binding site.

GTPase KRas precursor [Monodelphis domestica]

Nmr Data-driven Model Of Gtpase Kras-gdp Tethered To A Lipid-bilayer Nanodisc

Nmr Data-driven Model Of Gtpase Kras-gnp Tethered To A Lipid-bilayer Nanodisc

Nmr Data-driven Model Of Gtpase Kras-gnp:arafrbd Complex Tethered To A Lipid-bilayer Nanodisc

Human K-ras (q61h) In Complex With A Gtp Analogue

Small-Molecule Ligands Bind To A Distinct Pocket In Ras And Inhibit Sos-Mediated Nucleotide Exchange Activity

Discovery Of Small Molecules That Bind To K-Ras And Inhibit Sos- Mediated Activation

Crystal Structure Of K-ras G12c, Gdp-bound

Crystal Structure Of A Gdp-bound G12c Oncogenic Mutant Of Human Gtpase Kras

Crystal Structure Of K-ras Wt, Gdp-bound

Crystal Structure Of K-ras G12c (cysteine-light), Gdp-bound

Crystal Structure Of Small Molecule Disulfide 6 Bound To K-ras G12c

Crystal Structure Of Small Molecule Disulfide 4 Covalently Bound To K- Ras G12c

Crystal Structure Of Small Molecule Vinylsulfonamide 8 Covalently Bound To K-ras G12c

Crystal Structure Of Small Molecule Vinylsulfonamide 9 Covalently Bound To K-ras G12c

Crystal Structure Of Small Molecule Vinylsulfonamide 9 Covalently Bound To K-ras G12c, Alternative Space Group

Crystal Structure Of Small Molecule Vinylsulfonamide 7 Covalently Bound To K-ras G12c

Crystal Structure Of Small Molecule Vinylsulfonamide 13 Covalently Bound To K-ras G12c

Crystal Structure Of Small Molecule Vinylsulfonamide 14 Covalently Bound To K-ras G12c

Crystal Structure Of Small Molecule Vinylsulfonamide Covalently Bound To K-ras G12c

Crystal Structure Of Small Molecule Vinylsulfonamide 15 Covalently Bound To K-ras G12c

Crystal Structure Of Small Molecule Acrylamide 11 Covalently Bound To K-ras G12c

Crystal Structure Of Small Molecule Acrylamide 16 Covalently Bound To K-ras G12c

Crystal Structure Of A G12c Oncogenic Variant Of Human Kras Bound To A Novel Gdp Competitive Covalent Inhibitor

Crystal Structure Of Gdp-bound Human Kras

Second-site Screening Of K-ras In The Presence Of Covalently Attached First-site Ligands

Crystal Structure Of A Gdp-bound G12r Oncogenic Mutant Of Human Gtpase Kras

Crystal Structure Of A Gdp-bound G12v Oncogenic Mutant Of Human Gtpase Kras

Crystal Structure Of A Gdp-bound G13d Oncogenic Mutant Of Human Gtpase Kras

Crystal Structure Of A Gdp-bound Q61l Oncogenic Mutant Of Human Gt- Pase Kras

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [synthetic construct]

PR310 c-K-ras oncogene, partial [Homo sapiens]

PR371 c-K-ras oncogene, partial [Homo sapiens]

c-Ki-ras p21 protein, partial [Homo sapiens]

Ki-ras protein, partial [Rattus norvegicus]

ras-1 protein, partial [Oncorhynchus mykiss]

transforming protein c-Ki-ras-1, hepatic - rainbow trout (fragment)

uncharacterized protein LOC100505423 [Xenopus laevis]

RecName: Full=GTPase KRas; AltName: Full=Ki-Ras; Short=K-ras; Flags: Precursor

RecName: Full=GTPase KRas; AltName: Full=K-Ras 2; AltName: Full=Ki-Ras; Short=K-ras; Flags: Precursor

RecName: Full=GTPase KRas; AltName: Full=K-Ras 2; AltName: Full=Ki-Ras; AltName: Full=c-K-ras; AltName: Full=c-Ki-ras; Contains

TPA: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [Bos taurus]

This is a hybrid model generated by Haddock docking simulation that is based on
i) NMR data that includes PRE-measurements between lipids (i.e. nanodisc) and the protien (i.e. K-RasGNP and A-RafRBD)
ii) crystolagraphic information (K-RasGNP and A-RafRBD) and
iii) theoretical computation (i.e. nanodisc).

All PRE measurements were carrried out on the C-delta of Ile residues.

GTPase KRas-GDP tethered to a lipid-bilayer nanodisc
GTPase KRas-GNP tethered to a lipid-bilayer nanodisc
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (gadolinium salt)
CNS was used in all structure calculation using Haddock scripts
CHARMM-GUI (S. Jo, T. Kim, V.G. Iyer, and W. Im)
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Brunger, Adams, Clore, Gros, Nilges and Read