Protein A binding by an engineered Affibody molecule

Protein engineering, design & selection : PEDS
High-affinity binding to staphylococcal protein A by an engineered dimeric Affibody molecule.

An Affibody In Complex With A Target Protein: Structure And Coupled Folding

Protein Z In Complex With An In Vitro Selected Affibody

Refined Solution Nmr Structure Of The Z Domain Of Staphylococcal Protein A

Staphylococcal Protein A, Z-Domain, Nmr, 10 Structures

Structure Of The Mediator Head Module From S. Cerevisiae

Structure Of The Mediator Head Module From S. Cerevisiae In Complex With The Carboxy-Terminal Domain (Ctd) Of Rna Polymerase Ii

protein A doublet [Cloning vector pscFvCA-E8VHd]

protein A doublet [Cloning vector pFCAH9-E8d]

FLAG-TEV cut site-protein A tag [Cloning vector pCtFLATAki-kanMX6]

Spac323.08-FLATA [Targeting vector pCtFLATAki-Pspac323.08-SalI-spac323.08(+)-kanMX6T]

HA-TEV cut site-protein A tag [Schizosaccharomyces pombe expression vector pFOX1-CHATA]

staphylococcal protein A [synthetic construct]

bifunctional fusion protein, partial [synthetic construct]

protein A signal fusion protein [unidentified cloning vector]

ZZ:beta-Gal' IgG-binding fusion protein [Cloning vector pKP497]

C-terminal TAP tag [synthetic construct]

N-terminal TAP tag [synthetic construct]

hypothetical protein, partial [Staphylococcus aureus]

In-house standard expression vector with T7 promoter

2D F1(13C,15N)-filtered, F2(13C)-edited NOESY

13C,15N labeled Z domain with 25% molar excess ZpA963

Buffer: 75 mM NaCl, 20 mM sodium phosphate, 0.1 % azide, and 7.5 % D2O at pH 5.6 (uncorrected reading).

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax