binding to prodomains of bone morphogenetic proteins

H2O was used as the reference for 1H (4.75 ppm at 298K). The magnetogyric ratios were used for 13C and 15N.

¹H, ¹³C and ¹⁵N assignments of the four N-terminal domains of human fibrillin-1.

Pro-fibrillin sequence (pre-furin processing) from NCBI database: reference NP_000129.3

The four N-terminal domains of fibrillin-1: the fibrillin unique N-terminal
domain and the first three non-calcium binding epidermal growth factor-like
domains. Residues 1 and 2 are are spacer residues between a factor Xa cleavage
site and the native sequence.

1h, 13c And 15n Assignments Of The Four N-terminal Domains Of Human Fibrillin-1

fibrillin-1 precursor [Canis lupus familiaris]

fibrillin-1 precursor [Rattus norvegicus]

RecName: Full=Fibrillin-1; Flags: Precursor

RecName: Full=Fibrillin-1; AltName: Full=MP340; Flags: Precursor

Cells were also transformed with the pREP4 plasmid, enabling inducible expression

FUN-EGF3 is a region of the fibrillin-1 protein, which is encoded by the FBN1 gene

Chemical shift assignments for the FUN-EGF3 recombinant fragment of human fibrillin-1

home-built spectrometer with triple-axis gradient probe

simulated annealing, torsion angle dynamics
Ionic strength reported as 0. Lyophilised sample dissolved in H2O/D2O or D2O and adjusted to desired pH using NaOH and HCl or NaOD and DCl.
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax