Journal of the American Chemical Society
Coupled motion in proteins revealed by pressure perturbation.

backbone and sidechain order parameters for ubiquitin at 1, 400, 800, 1200, 1600 and 2500 bar

The order parameters of backbone and sidechains were determined using 15N T1,T2,NOE relaxation and 2H IzCzDz, IzCz and IzCzDy relaxation experiments at 1, 400, 800, 1200, 1600 and 2500 bar.

The sample contained 1.2 mM 100%13C-55%2H labeled and 0.8 mM 100%15N labeled ubiquitin