The structure is of a model peptide (TM1b(1-19)Zip) of the N terminus of
non-muscle,low molecular weight rat alpha tropomyosin in complex with a model
peptide of the C terminus of non-muscle/smooth muscle tropomyosins,
Tm9d(252-284). TM1b(1-19)Zip is residues 1-19 encoded by exon 1b of the TPM1
gene followed by the last 18 residues of the yeast transcription factor, GCN4.
Gly replaces the native N-terminal acetyl group. The C-terminal model contains
residues 252-284 encoded by exon 8 and exon 9d with Gly-Cys-Gly-Gly at the N
terminus to allow oxidatitive cross-linking. The TM1b(1-19)Zip is labeled with
either 15N or 15N and 13C. TM9d(252-284) is unlabeled.

intermolecular junction between low molecular weight non-muscle tropomyosin molecules

insert at center of experimental sample tube

Structure of the N terminus of a nonmuscle alpha-tropomyosin in complex with the C terminus: implications for actin binding.

0-37 (sequence code)
1-38 (residue code, used in NMR determination)

Residue 0 is a glycine group replacing a native acetyl group residue 1-19 are
the first 19 residues of a rat non-muscle low molecular weight tropomyosin
encoded by exon 1b (TPM1 gene). 0 is a glycine residue that substitutes for
the native acetyl group. Residues 20-37 are the last 18 residues of the yeast
transcription factor GCN4.

There is no NMR structural information on this molecule. It is unlabeled and is not in the PDB file.

248-284 sequence code
1-36 residue code

Residues 252-284 are encoded by exon 8 and 9d or rat alpha tropomyosin (TPM1 gene).
Residues 248-251, GCGG were added to allow disulfide cross linking.

forms intermolecular junction with the C terminus of neighboring tropomyosin molecule

actin binding site, part of intramolecule junction between tropomyosin molecules

Glytm1bzip: A Chimeric Peptide Model Of The N-Terminus Of A Rat Short Alpha Tropomyosin With The N-Terminus Encoded By Exon 1b

Glytm1b(1-19)zip: A Chimeric Peptide Model Of The N-Terminus Of A Rat Short Alpha-Tropomyosin With The N-Terminus Encoded By Ex

Crystal Structure Of Tropomyosin N-Terminal Fragment At 0.98a Resolution

tropomyosin alpha-1 chain [synthetic construct]

alpha-tropomyosin 5a [Rattus norvegicus]

alpha-tropomyosin 5b [Rattus norvegicus]

brain alpha-tropomyosin (TMBr-2) [Rattus norvegicus]

tropomyosin alpha-1 chain isoform Tpm1.12br [Homo sapiens]

tropomyosin alpha-1 chain isoform Tpm1.8cy [Rattus norvegicus]

tropomyosin alpha-1 chain isoform Tpm1.9cy [Rattus norvegicus]

tropomyosin alpha-1 chain isoform Tpm1.13 [Rattus norvegicus]

tropomyosin alpha-1 chain isoform Tpm1.8cy [Mus musculus]

Structure Of The Tropomyosin Overlap Complex From Chicken Smooth Muscle

unnamed protein product [Macaca fascicularis]

unnamed protein product [Rattus norvegicus]

tropomyosin alpha chain, smooth muscle - rat

tropomyosin alpha-1 chain isoform Tpm1.6cy [Homo sapiens]

tropomyosin alpha-1 chain isoform Tpm1.7cy [Homo sapiens]

tropomyosin alpha-1 chain isoform Tpm1.4sm [Homo sapiens]

tropomyosin alpha-1 chain isoform Tpm1.3sm [Homo sapiens]

tropomyosin alpha-1 chain isoform Tpm1.6cy [Rattus norvegicus]

Residues 1-19 are the first 19 of encoded by exon 1b.

Residues 252-284 are encoded by part of exon 8 and all of exon 9d

The conformational changes in the N-terminal domain of tropomyosin upon binding to the C-terminal domain to form an "overlap" complex.
Structural Transitions of the N Terminus of a Short Non-Muscle Tropomyosin upon Binding to the C Terminus in Solution

DGSA-distance geometry simulated annealing

Solution NMR Structure and Folding Dynamics of TM1bZip

structure of unbound TM1b(1-19)Zip, a peptide model of the N terminus of non-muscle tropomyosin

13C, 15N TM1b(1-19)Zip + umlabled TM9d(252-284)
100 mM NaCl, 10 mM sodium phosphate, pH 6.4

15N TM1b(1-19)Zip + umlabeled TM9d(252-284)
100 mM NaCl, 10 mM sodium phosphate, pH 6.4

heterodimer of 13C, 15N TM1b(1-19)Zip + unlabeled TM1b(1-19)Zip and unlabeled TM9d(252-284)
100 mM NaCl, 10 mM sodium phosphate, pH 6.4

Northeast Structural Genomics Consortium

CABM, Rutgers University, Piscataway, NJ

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Brunger, Adams, Clore, Gros, Nilges and Read