2D 1H-13C HSQC high res. (L/V stereoassignment)

SOLUTION NMR STRUCTURE OF SAG0934 from Streptococcus agalactiae. NORTHEAST STRUCTURAL GENOMICS TARGET SaR32[1-108].

C-terminal LEHHHHHH purification tag starting at residue 109.
The construct was optimized by Hydrogen/Deuterium exchange mass spectrometry (DXMS)

Solution Nmr Structure Of Sag0934 From Streptococcus Agalactiae. Northeast Structural Genomics Target Sar32[1- 108]

hypothetical protein [Streptococcus pneumoniae]

hypothetic protein [Streptococcus parauberis]

Tn916 ORF22 protein [Streptococcus gallolyticus subsp. gallolyticus ATCC 43143]

hypothetical protein ANG2_0024 [Streptococcus constellatus subsp. constellatus SK53]

conjugative transposon protein [Staphylococcus aureus subsp. aureus ST398]

conjugative transposon protein [Streptococcus pneumoniae ATCC 700669]

hypothetical protein SSUSC84_0837 [Streptococcus suis SC84]

hypothetical protein SSUBM407_0493 [Streptococcus suis BM407]

Tn916, hypothetical protein [Streptococcus agalactiae 2603V/R]

hypothetical protein [Streptococcus cristatus]

hypothetical protein SSU05_0932 [Streptococcus suis 05ZYH33]

hypothetical protein SSU98_0938 [Streptococcus suis 98HAH33]

Tn916 hypothetical protein [Streptococcus agalactiae 2603V/R]

MULTISPECIES: hypothetical protein [Bacteria]

hypothetical protein [Enterococcus faecium]

transposase [Peptostreptococcus anaerobius]

The full length protein is 128 amino acids

CN-double labeled SaR32 NMR sample in water

CN-double labeled SaR32 NMR sample in D2O

Northeast Structural Genomics Consortium

Zimmerman, Moseley, Kulikowski and Montelione

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Tejero, Snyder, Mao, Aramini and Montelione

Brunger, Adams, Clore, Gros, Nilges and Read