3D 13C-F1-filtered 13C-F3-separateded NOESY

An engineered second disulfide bond restricts lymphotactin/XCL1 to a chemokine-like conformation with XCR1 agonist activity.

Solution Structure Of Human Lymphotactin

Solution Structure Of V21cV59C LYMPHOTACTINXCL1

Solution Structure Of The Alternative Conformation Of Xcl1LYMPHOTACTIN

Solution Structure Of A Disulfide Stabilized Xcl1 Dimer

Viral Chemokine Binding Protein M3 From Murine Gammaherpesvirus68 In Complex With The C- Chemokine Xcl1

chemokine (C motif) ligand 2 [synthetic construct]

CD8+T cell specific protein [Homo sapiens]

Chemokine (C motif) ligand 2 [Homo sapiens]

Chemokine (C motif) ligand 1 [Homo sapiens]

cytokine SCM-1 beta precursor [Homo sapiens]

PREDICTED: lymphotactin-like [Gorilla gorilla gorilla]

PREDICTED: lymphotactin [Pan troglodytes]

RecName: Full=Lymphotactin; AltName: Full=ATAC; AltName: Full=C motif chemokine 1; AltName: Full=Cytokine SCM-1; AltName: Full=

RecName: Full=Cytokine SCM-1 beta; AltName: Full=C motif chemokine 2; AltName: Full=XC chemokine ligand 2; Flags: Precursor

Solution structure of the alternative conformation of XCL1/Lymphotactin

AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT. FINAL STRUCTURES WERE OBTAINED BY MOLECULAR DYNAMICS IN EXPLICIT SOLVENT
1.0 mM XCL1 U-15N/13C, 20 mM sodium phosphate
0.6 mM XCL1 U-15N/13C, 0.6 mM XCL1 unlabeled, 20 mM sodium phosphate
Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax