single chain polypeptide without ligands

separate tube (no insert) similar to the experimental sample tube

Glycerol-induced folding of unstructured disulfide-deficient lysozyme into a native-like conformation.

Disulfide-free variant of hen lysozyme: 0SS

two-disulfide variant of lysozyme 2SS[6-127, 64-80] with Cys6-Cys127 and
Cys64-Cys80

The pH was adjusted by adding DCl and NaOD.