Kissing complex formed by the apical loop of the HIV TAR RNA and a high-affinity
RNA aptamer optimized by SELEX.

Interaction of TAR with TAR*GA aptamer : inhibition of HIV transcription

Proceedings of the National Academy of Sciences of the United States of America
Liquid-crystal NMR structure of HIV TAR RNA bound to its SELEX RNA aptamer reveals the origins of the high stability of the complex.

high stability RNA aptamer selected by SELEX

This sequence is the apical loop of the HIV TAR RNA, stabilized by a GC
base-pair.

The numbering begins at 17: G17 C18 U19 G20 G21 U22 C23 C24 C25 A26 G27 A28 C29
A30 G31 C32.

Apical loop of the Trans Activation Response RNA in HIV

High affinity RNA aptamer optimized to bind the apical loop of HIV TAR by SELEX.

Natural abundance RNA were synthesized chemically by Integrated DNA Technologies
, BVBA. 13C-15N-labelled RNA were prepared in vitro using T7 RNA polymerase by
Silantes GmbH.

Apical loop of the Trans Activation Response RNA (TAR) of HIV-1, stabilized by a
GC base pair.

RNA aptamer optimized to bind the apical loop of the HIV TAR RNA by SELEX.

Liquid crystal solution structure of the kissing complex formed by the apical
loop of the HIV TAR RNA and a high affinity RNA aptamer optimized by SELEX.

University of Ottawa, Chemistry department

Lower affinity aptamer - HIV TAR kissing complex

Solution containing Pf1 filamentous phages (D2O splitting =12.4 Hz).

Solution containing Pf1 filamentous phages (D2O splitting =14.0 Hz).

Force field optimized for nucleic acids (Bryce et al., JACS 2005).

High affinity RNA aptamer selected by SELEX

Schwieters, Kuszewski, Tjandra and Clore

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax