This sample is a symmetric homodimer, in which the same residues in each monomer
generate the same signals, thus the spectrum seems of the monomer.

bovine beta-lactoglobulin A34C; disulfide linked dimer

Dynamics and mechanism of the Tanford transition of bovine beta-lactoglobulin studied using heteronuclear NMR spectroscopy.

Kim, T.-R., Goto, Y., Hirota, N., Kuwata, K., Denton, H., Wu, S.-Y., Sawyer, L.,
and Batt, C.A. (1997)
High-level expression of bovine beta-lactoglobulin in Pichia pastoris and
characterization of its physical properties.
Protein Eng. 10:1339-1345.

High-level expression of bovine beta-lactoglobulin in Pichia pastoris and characterization of its physical properties.

Kuwata, K., Hoshino, M., Era, S., Batt, C.A., and Goto, Y. (1998)
alpha to beta Transition of beta-lactoglobulin as evidenced by heteronuclear NMR.
J. Mol. Biol. 283: 731-739.

alpha-->beta transition of beta-lactoglobulin as evidenced by heteronuclear NMR.

Uhrinova, S., Uhrin, D., Denton, H., Smith, M., Sawyer, L., and Barlow, P.N.
(1998)
Complete assignment of 1H, 13C and 15N chemical shifts for bovine
beta-lactoglobulin: Secondary structure and topology of the native state is
retained in a partially unfolded form.
J. Biomol. NMR 12: 89-107.

Complete assignment of 1H, 13C and 15N chemical shifts for bovine beta-lactoglobulin: secondary structure and topology of the native state is retained in a partially unfolded form.

The first three residues are signal sequence necessary for the expression.
The native sequence numbering starts at the forth residue.
-3 EAE -1
1 AYVTQ TMKGL DIQKV AGTWY 20
21 SLAMA ASDIS LLDAQ SAPLR 40
41 VYVEE LKPTP EGDLE ILLQK 60
61 WENDE CAQKK IIAEK TKIPA 80
81 VFKID ALNEN KVLVL DTDYK 100
101 KYLLF CMENS AEPEQ SLVCQ 120
121 CLVRT PEVDD EALEK FDKAL 140
141 KALPM HIRLS FNPTQ LEEQC 160
161 HI 162

Bovine Beta-Lactoglobulin Complexed With Palmitate, Lattice Z

High Resolution Crystal Structure Of The Bovine Beta- Lactoglobulin (Isoforms A And B) In Orthorombic Space Group

12-Bromododecanoic Acid Binds Inside The Calyx Of Bovine Beta-Lactoglobulin

Structural And Functional Consequences Of Point Mutations Of Variants A And B Of Bovine Beta-Lactoglobulin

Structural Basis Of The Tanford Transition Of Bovine Beta- Lactoglobulin From Crystal Structures At Three Ph Values; Ph 7.1

Structural Changes Accompanying Ph-Induced Dissociation Of The B-Lactoglobulin Dimer

Bovine Beta-Lactoglobulin Complexed With Retinol, Trigonal Lattice Z

Bovine Beta-Lactoglobulin Complexed With Retinoic Acid, Trigonal Lattice Z

Bovine Beta-Lactoglobulin Complexed With Retinol And Palmitic Acid, Trigonal Lattice Z

High Resolution Crystal Structure Of The Bovine Beta- Lactoglobulin (Isoform A)

The Cys121ser Mutant Of Beta-lactoglobulin

The Structure Of Bovine B-Lactoglobulin A In Crystals Grown At Very Low Ionic Strength

Structural Basis Of The Tanford Transition Of Bovine Beta- Lactoglobulin From Crystal Structures At Three Ph Values; Ph 8.2

Crystal Structure Of A Secondary Vitamin D3 Binding Site Of Milk Beta-Lactoglobulin

Crystal Structure Of A Recombinant Ige Fab Fragment In Complex With Bovine Beta-Lactoglobulin Allergen

Structural Basis Of The Tanford Transition Of Bovine Beta- Lactoglobulin From Crystal Structures At Three Ph Values; Ph 6.2

Bovine Beta Lactoglobulin Unliganded Form

Bovine Beta-Lactoglobulin Complex With Capric Acid

Bovine Beta-Lactoglobulin Complex With Caprylic Acid

Bovine Beta Lactoglobulin Crystallized Through Ligandation Of Yttrium Cations

Bovine Beta Lactoglobulin Crytsallized Through Ligandation Of Yttrium

Bovine Beta-Lactoglobulin Complex With Lauric Acid

Bovine Beta-Lactoglobulin Complex With Myristic Acid

Bovine Beta-Lactoglobulin Complex With Palmitic Acid

Bovine Beta-Lactoglobulin Complex With Stearic Acid

Bovine Beta-lactoglobulin Complex With Oleic Acid

Bovine Beta-lactoglobulin Complex With Linoleic Acid

Bovine Beta-lactoglobulin Complex With Dodecyl Sulfate

Bovine Beta-lactoglobulin (isoform A) In Complex With Lauric Acid (c12)

Bovine Beta-lactoglobulin (isoform A) In Complex With Dodecyltrimethylammonium (dtac)

Bovine Beta-lactoglobulin (isoform A) In Complex With Dodecyl Sulphate (sds)

Bovine Beta-lactoglobulin (isoform B) In Complex With Dodecyltrimethylammonium (dtac)

Bovine Beta-lactoglobulin (isoform B) In Complex With Dodecyl Sulphate (sds)

Beta-lactoglobulin In Complex With Cp*rh(iii)cl N,n-di(pyridin-2-yl) Dodecanamide

Bovine Beta-lactoglobulin Crystallized In The Presence Of 2 Mm Zinc Chloride

Bovine Beta-lactoglobulin Crystallized In The Presence Of 20 Mm Zinc Chloride

Bovine Beta-lactoglobulin Complex With Tetracaine (blg-tet)

Bovine Beta-lactoglobulin Complex With Pramocaine Crystallized From Sodium Citrate (blg-prm1)

Bovine Beta-lactoglobulin Complex With Pramocaine Crystallized From Ammonium Sulphate (blg-prm2)

beta-lactoglobulin variant B precursor [Bos taurus]

beta-lactoglobulin, partial [Bos taurus]

major allergen beta-lactoglobulin [Bos taurus]

beta-lactoglobulin, partial [Bos grunniens]

beta-lactoglobulin precursor [Bubalus bubalis]

beta-lactoglobulin precursor [Bos taurus]

PREDICTED: beta-lactoglobulin isoform X1 [Bos mutus]

PREDICTED: beta-lactoglobulin-like isoform X2 [Bubalus bubalis]

RecName: Full=Beta-lactoglobulin; Short=Beta-LG; AltName: Allergen=Bos d 5; Flags: Precursor

RecName: Full=Beta-lactoglobulin; Short=Beta-LG; Flags: Precursor

TPA: beta-lactoglobulin precursor [Bos taurus]

Laboratory of Protein Folding, Inst. Protein Res., Osaka-Univ.

Protein dissolved into water.
(No buffer is added.)
pH is adjusted by adding HCl.

NMR measurement of bovine beta-lactoglobulin at neutral pH