data_53577 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 53577 _Entry.Title ; UP1 in complex with UTR-Z from 5' KRAS UTR ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2026-02-17 _Entry.Accession_date 2026-02-17 _Entry.Last_release_date 2026-02-17 _Entry.Original_release_date 2026-02-17 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID 1 _Entry.Generated_software_label $software_1 _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details '{1H-15N} TROSY titration of UP1 fragment from HNRNPA1 with RNA G-quadruplex UTR-Z from KRAS mRNA' _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Gilmar Salgado . . . 0000-0002-0296-5979 53577 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2026-03-18 . original BMRB . 53577 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 53573 'UP1 {1H-15N} HSQC at 25 degC' 53577 BMRB 53574 "UP1 in complex with UTR-1 from 5' KRAS UTR" 53577 BMRB 53578 "UP1 in complex with UTR-C from 5'-UTR mRNA of KRAS" 53577 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 53577 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID . _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Recognition and Resolution of KRAS 5'UTR RNA G-Quadruplexes by hnRNPA1 ; _Citation.Status submitted _Citation.Type journal _Citation.Journal_abbrev iScience _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Gilmar Salgado . . . . 53577 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 53577 _Assembly.ID 1 _Assembly.Name "UP1 in complex with UTR-Z from 5' KRAS UTR mRNA" _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states yes _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'protein (UP1)' 1 $entity_1 . . yes native no no . . . 53577 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 53577 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GPKEPEQLRKLFIGGLSFET TDESLRSHFEQWGTLTDCVV MRDPNTKRSRGFGFVTYATV EEVDAAMNARPHKVDGRVVE PKRAVSREDSQRPGAHLTVK KIFVGGIKEDTEEHHLRDYF EQYGKIEVIEIMTDRGSGKK RGFAFVTFDDHDSVDKIVIQ KYHTVNGHNCEVRKALSKQE MASAS ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states yes _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 185 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state unknown _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment UP1 _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . GLY . 53577 1 2 . PRO . 53577 1 3 . LYS . 53577 1 4 . GLU . 53577 1 5 . PRO . 53577 1 6 . GLU . 53577 1 7 . GLN . 53577 1 8 . LEU . 53577 1 9 . ARG . 53577 1 10 . LYS . 53577 1 11 . LEU . 53577 1 12 . PHE . 53577 1 13 . ILE . 53577 1 14 . GLY . 53577 1 15 . GLY . 53577 1 16 . LEU . 53577 1 17 . SER . 53577 1 18 . PHE . 53577 1 19 . GLU . 53577 1 20 . THR . 53577 1 21 . THR . 53577 1 22 . ASP . 53577 1 23 . GLU . 53577 1 24 . SER . 53577 1 25 . LEU . 53577 1 26 . ARG . 53577 1 27 . SER . 53577 1 28 . HIS . 53577 1 29 . PHE . 53577 1 30 . GLU . 53577 1 31 . GLN . 53577 1 32 . TRP . 53577 1 33 . GLY . 53577 1 34 . THR . 53577 1 35 . LEU . 53577 1 36 . THR . 53577 1 37 . ASP . 53577 1 38 . CYS . 53577 1 39 . VAL . 53577 1 40 . VAL . 53577 1 41 . MET . 53577 1 42 . ARG . 53577 1 43 . ASP . 53577 1 44 . PRO . 53577 1 45 . ASN . 53577 1 46 . THR . 53577 1 47 . LYS . 53577 1 48 . ARG . 53577 1 49 . SER . 53577 1 50 . ARG . 53577 1 51 . GLY . 53577 1 52 . PHE . 53577 1 53 . GLY . 53577 1 54 . PHE . 53577 1 55 . VAL . 53577 1 56 . THR . 53577 1 57 . TYR . 53577 1 58 . ALA . 53577 1 59 . THR . 53577 1 60 . VAL . 53577 1 61 . GLU . 53577 1 62 . GLU . 53577 1 63 . VAL . 53577 1 64 . ASP . 53577 1 65 . ALA . 53577 1 66 . ALA . 53577 1 67 . MET . 53577 1 68 . ASN . 53577 1 69 . ALA . 53577 1 70 . ARG . 53577 1 71 . PRO . 53577 1 72 . HIS . 53577 1 73 . LYS . 53577 1 74 . VAL . 53577 1 75 . ASP . 53577 1 76 . GLY . 53577 1 77 . ARG . 53577 1 78 . VAL . 53577 1 79 . VAL . 53577 1 80 . GLU . 53577 1 81 . PRO . 53577 1 82 . LYS . 53577 1 83 . ARG . 53577 1 84 . ALA . 53577 1 85 . VAL . 53577 1 86 . SER . 53577 1 87 . ARG . 53577 1 88 . GLU . 53577 1 89 . ASP . 53577 1 90 . SER . 53577 1 91 . GLN . 53577 1 92 . ARG . 53577 1 93 . PRO . 53577 1 94 . GLY . 53577 1 95 . ALA . 53577 1 96 . HIS . 53577 1 97 . LEU . 53577 1 98 . THR . 53577 1 99 . VAL . 53577 1 100 . LYS . 53577 1 101 . LYS . 53577 1 102 . ILE . 53577 1 103 . PHE . 53577 1 104 . VAL . 53577 1 105 . GLY . 53577 1 106 . GLY . 53577 1 107 . ILE . 53577 1 108 . LYS . 53577 1 109 . GLU . 53577 1 110 . ASP . 53577 1 111 . THR . 53577 1 112 . GLU . 53577 1 113 . GLU . 53577 1 114 . HIS . 53577 1 115 . HIS . 53577 1 116 . LEU . 53577 1 117 . ARG . 53577 1 118 . ASP . 53577 1 119 . TYR . 53577 1 120 . PHE . 53577 1 121 . GLU . 53577 1 122 . GLN . 53577 1 123 . TYR . 53577 1 124 . GLY . 53577 1 125 . LYS . 53577 1 126 . ILE . 53577 1 127 . GLU . 53577 1 128 . VAL . 53577 1 129 . ILE . 53577 1 130 . GLU . 53577 1 131 . ILE . 53577 1 132 . MET . 53577 1 133 . THR . 53577 1 134 . ASP . 53577 1 135 . ARG . 53577 1 136 . GLY . 53577 1 137 . SER . 53577 1 138 . GLY . 53577 1 139 . LYS . 53577 1 140 . LYS . 53577 1 141 . ARG . 53577 1 142 . GLY . 53577 1 143 . PHE . 53577 1 144 . ALA . 53577 1 145 . PHE . 53577 1 146 . VAL . 53577 1 147 . THR . 53577 1 148 . PHE . 53577 1 149 . ASP . 53577 1 150 . ASP . 53577 1 151 . HIS . 53577 1 152 . ASP . 53577 1 153 . SER . 53577 1 154 . VAL . 53577 1 155 . ASP . 53577 1 156 . LYS . 53577 1 157 . ILE . 53577 1 158 . VAL . 53577 1 159 . ILE . 53577 1 160 . GLN . 53577 1 161 . LYS . 53577 1 162 . TYR . 53577 1 163 . HIS . 53577 1 164 . THR . 53577 1 165 . VAL . 53577 1 166 . ASN . 53577 1 167 . GLY . 53577 1 168 . HIS . 53577 1 169 . ASN . 53577 1 170 . CYS . 53577 1 171 . GLU . 53577 1 172 . VAL . 53577 1 173 . ARG . 53577 1 174 . LYS . 53577 1 175 . ALA . 53577 1 176 . LEU . 53577 1 177 . SER . 53577 1 178 . LYS . 53577 1 179 . GLN . 53577 1 180 . GLU . 53577 1 181 . MET . 53577 1 182 . ALA . 53577 1 183 . SER . 53577 1 184 . ALA . 53577 1 185 . SER . 53577 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 53577 1 . PRO 2 2 53577 1 . LYS 3 3 53577 1 . GLU 4 4 53577 1 . PRO 5 5 53577 1 . GLU 6 6 53577 1 . GLN 7 7 53577 1 . LEU 8 8 53577 1 . ARG 9 9 53577 1 . LYS 10 10 53577 1 . LEU 11 11 53577 1 . PHE 12 12 53577 1 . ILE 13 13 53577 1 . GLY 14 14 53577 1 . GLY 15 15 53577 1 . LEU 16 16 53577 1 . SER 17 17 53577 1 . PHE 18 18 53577 1 . GLU 19 19 53577 1 . THR 20 20 53577 1 . THR 21 21 53577 1 . ASP 22 22 53577 1 . GLU 23 23 53577 1 . SER 24 24 53577 1 . LEU 25 25 53577 1 . ARG 26 26 53577 1 . SER 27 27 53577 1 . HIS 28 28 53577 1 . PHE 29 29 53577 1 . GLU 30 30 53577 1 . GLN 31 31 53577 1 . TRP 32 32 53577 1 . GLY 33 33 53577 1 . THR 34 34 53577 1 . LEU 35 35 53577 1 . THR 36 36 53577 1 . ASP 37 37 53577 1 . CYS 38 38 53577 1 . VAL 39 39 53577 1 . VAL 40 40 53577 1 . MET 41 41 53577 1 . ARG 42 42 53577 1 . ASP 43 43 53577 1 . PRO 44 44 53577 1 . ASN 45 45 53577 1 . THR 46 46 53577 1 . LYS 47 47 53577 1 . ARG 48 48 53577 1 . SER 49 49 53577 1 . ARG 50 50 53577 1 . GLY 51 51 53577 1 . PHE 52 52 53577 1 . GLY 53 53 53577 1 . PHE 54 54 53577 1 . VAL 55 55 53577 1 . THR 56 56 53577 1 . TYR 57 57 53577 1 . ALA 58 58 53577 1 . THR 59 59 53577 1 . VAL 60 60 53577 1 . GLU 61 61 53577 1 . GLU 62 62 53577 1 . VAL 63 63 53577 1 . ASP 64 64 53577 1 . ALA 65 65 53577 1 . ALA 66 66 53577 1 . MET 67 67 53577 1 . ASN 68 68 53577 1 . ALA 69 69 53577 1 . ARG 70 70 53577 1 . PRO 71 71 53577 1 . HIS 72 72 53577 1 . LYS 73 73 53577 1 . VAL 74 74 53577 1 . ASP 75 75 53577 1 . GLY 76 76 53577 1 . ARG 77 77 53577 1 . VAL 78 78 53577 1 . VAL 79 79 53577 1 . GLU 80 80 53577 1 . PRO 81 81 53577 1 . LYS 82 82 53577 1 . ARG 83 83 53577 1 . ALA 84 84 53577 1 . VAL 85 85 53577 1 . SER 86 86 53577 1 . ARG 87 87 53577 1 . GLU 88 88 53577 1 . ASP 89 89 53577 1 . SER 90 90 53577 1 . GLN 91 91 53577 1 . ARG 92 92 53577 1 . PRO 93 93 53577 1 . GLY 94 94 53577 1 . ALA 95 95 53577 1 . HIS 96 96 53577 1 . LEU 97 97 53577 1 . THR 98 98 53577 1 . VAL 99 99 53577 1 . LYS 100 100 53577 1 . LYS 101 101 53577 1 . ILE 102 102 53577 1 . PHE 103 103 53577 1 . VAL 104 104 53577 1 . GLY 105 105 53577 1 . GLY 106 106 53577 1 . ILE 107 107 53577 1 . LYS 108 108 53577 1 . GLU 109 109 53577 1 . ASP 110 110 53577 1 . THR 111 111 53577 1 . GLU 112 112 53577 1 . GLU 113 113 53577 1 . HIS 114 114 53577 1 . HIS 115 115 53577 1 . LEU 116 116 53577 1 . ARG 117 117 53577 1 . ASP 118 118 53577 1 . TYR 119 119 53577 1 . PHE 120 120 53577 1 . GLU 121 121 53577 1 . GLN 122 122 53577 1 . TYR 123 123 53577 1 . GLY 124 124 53577 1 . LYS 125 125 53577 1 . ILE 126 126 53577 1 . GLU 127 127 53577 1 . VAL 128 128 53577 1 . ILE 129 129 53577 1 . GLU 130 130 53577 1 . ILE 131 131 53577 1 . MET 132 132 53577 1 . THR 133 133 53577 1 . ASP 134 134 53577 1 . ARG 135 135 53577 1 . GLY 136 136 53577 1 . SER 137 137 53577 1 . GLY 138 138 53577 1 . LYS 139 139 53577 1 . LYS 140 140 53577 1 . ARG 141 141 53577 1 . GLY 142 142 53577 1 . PHE 143 143 53577 1 . ALA 144 144 53577 1 . PHE 145 145 53577 1 . VAL 146 146 53577 1 . THR 147 147 53577 1 . PHE 148 148 53577 1 . ASP 149 149 53577 1 . ASP 150 150 53577 1 . HIS 151 151 53577 1 . ASP 152 152 53577 1 . SER 153 153 53577 1 . VAL 154 154 53577 1 . ASP 155 155 53577 1 . LYS 156 156 53577 1 . ILE 157 157 53577 1 . VAL 158 158 53577 1 . ILE 159 159 53577 1 . GLN 160 160 53577 1 . LYS 161 161 53577 1 . TYR 162 162 53577 1 . HIS 163 163 53577 1 . THR 164 164 53577 1 . VAL 165 165 53577 1 . ASN 166 166 53577 1 . GLY 167 167 53577 1 . HIS 168 168 53577 1 . ASN 169 169 53577 1 . CYS 170 170 53577 1 . GLU 171 171 53577 1 . VAL 172 172 53577 1 . ARG 173 173 53577 1 . LYS 174 174 53577 1 . ALA 175 175 53577 1 . LEU 176 176 53577 1 . SER 177 177 53577 1 . LYS 178 178 53577 1 . GLN 179 179 53577 1 . GLU 180 180 53577 1 . MET 181 181 53577 1 . ALA 182 182 53577 1 . SER 183 183 53577 1 . ALA 184 184 53577 1 . SER 185 185 53577 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 53577 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 53577 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 53577 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pGEX-UP1 . . . 53577 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 53577 _Sample.ID 1 _Sample.Name "UP1 in complex (1:1) with UTR-Z from 5' KRAS UTR RNA G-quadruplex" _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'protein (UP1)' '[U-98% 15N]' . . 1 $entity_1 . . 55 50 60 uM 0.1 . . . 53577 1 2 'RNA (UTR-Z)' '[U-98% 15N]' . . . . . . 55 50 60 uM 0.1 . . . 53577 1 3 KPi 'natural abundance' . . . . . . 10 . . mM . . . . 53577 1 4 KCl 'natural abundance' . . . . . . 50 . . mM . . . . 53577 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 53577 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Native UP1 KPi buffer' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 0.060 . M 53577 1 pH 6.8 . pH 53577 1 pressure 1 . atm 53577 1 temperature 298 . K 53577 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 53577 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version 3.6 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 53577 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 53577 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 800 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE NEO' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 53577 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' yes no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53577 1 stop_ loop_ _Experiment_file.Experiment_ID _Experiment_file.Experiment_name _Experiment_file.Name _Experiment_file.Type _Experiment_file.Content _Experiment_file.Directory_path _Experiment_file.Details _Experiment_file.Entry_ID _Experiment_file.Experiment_list_ID 1 '2D 1H-15N HSQC' 2rr . 'Time-domain (raw spectral data)' . . 53577 1 stop_ save_