data_53309 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 53309 _Entry.Title ; Amide backbone assignment of DNAJB1 Y5D Y6D ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2025-08-08 _Entry.Accession_date 2025-08-08 _Entry.Last_release_date 2025-08-08 _Entry.Original_release_date 2025-08-08 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID 1 _Entry.Generated_software_label $software_1 _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Anna Leder . . . . 53309 2 Guillaume Mas . . . . 53309 3 Sebastian Hiller . . . . 53309 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 53309 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 73 53309 '1H chemical shifts' 73 53309 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2025-11-06 . original BMRB . 53309 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 53309 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID . _Citation.DOI . _Citation.Full_citation . _Citation.Title ; A universal tyrosine-based regulatory switch controls J-domain protein activity ; _Citation.Status 'in preparation' _Citation.Type journal _Citation.Journal_abbrev 'Nat. Comm.' _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Anna Leder . . . . 53309 1 2 Guillaume Mas . . . . 53309 1 3 Sebastian Hiller . . . . 53309 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 53309 _Assembly.ID 1 _Assembly.Name 'DNAJB1 J-domain Y5D Y6D' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'DNAJB1 J-domain Y5D Y6D' 1 $entity_1 . . yes native no no . . . 53309 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 53309 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MGKDDDQTLGLARGASDEEI KRAYRRQALRYHPDKNKEPG AEEKFKEIAEAYDVLSDPRK REIFDRYGEEGLKGSGC ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 77 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 53309 1 2 . GLY . 53309 1 3 . LYS . 53309 1 4 . ASP . 53309 1 5 . ASP . 53309 1 6 . ASP . 53309 1 7 . GLN . 53309 1 8 . THR . 53309 1 9 . LEU . 53309 1 10 . GLY . 53309 1 11 . LEU . 53309 1 12 . ALA . 53309 1 13 . ARG . 53309 1 14 . GLY . 53309 1 15 . ALA . 53309 1 16 . SER . 53309 1 17 . ASP . 53309 1 18 . GLU . 53309 1 19 . GLU . 53309 1 20 . ILE . 53309 1 21 . LYS . 53309 1 22 . ARG . 53309 1 23 . ALA . 53309 1 24 . TYR . 53309 1 25 . ARG . 53309 1 26 . ARG . 53309 1 27 . GLN . 53309 1 28 . ALA . 53309 1 29 . LEU . 53309 1 30 . ARG . 53309 1 31 . TYR . 53309 1 32 . HIS . 53309 1 33 . PRO . 53309 1 34 . ASP . 53309 1 35 . LYS . 53309 1 36 . ASN . 53309 1 37 . LYS . 53309 1 38 . GLU . 53309 1 39 . PRO . 53309 1 40 . GLY . 53309 1 41 . ALA . 53309 1 42 . GLU . 53309 1 43 . GLU . 53309 1 44 . LYS . 53309 1 45 . PHE . 53309 1 46 . LYS . 53309 1 47 . GLU . 53309 1 48 . ILE . 53309 1 49 . ALA . 53309 1 50 . GLU . 53309 1 51 . ALA . 53309 1 52 . TYR . 53309 1 53 . ASP . 53309 1 54 . VAL . 53309 1 55 . LEU . 53309 1 56 . SER . 53309 1 57 . ASP . 53309 1 58 . PRO . 53309 1 59 . ARG . 53309 1 60 . LYS . 53309 1 61 . ARG . 53309 1 62 . GLU . 53309 1 63 . ILE . 53309 1 64 . PHE . 53309 1 65 . ASP . 53309 1 66 . ARG . 53309 1 67 . TYR . 53309 1 68 . GLY . 53309 1 69 . GLU . 53309 1 70 . GLU . 53309 1 71 . GLY . 53309 1 72 . LEU . 53309 1 73 . LYS . 53309 1 74 . GLY . 53309 1 75 . SER . 53309 1 76 . GLY . 53309 1 77 . CYS . 53309 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 53309 1 . GLY 2 2 53309 1 . LYS 3 3 53309 1 . ASP 4 4 53309 1 . ASP 5 5 53309 1 . ASP 6 6 53309 1 . GLN 7 7 53309 1 . THR 8 8 53309 1 . LEU 9 9 53309 1 . GLY 10 10 53309 1 . LEU 11 11 53309 1 . ALA 12 12 53309 1 . ARG 13 13 53309 1 . GLY 14 14 53309 1 . ALA 15 15 53309 1 . SER 16 16 53309 1 . ASP 17 17 53309 1 . GLU 18 18 53309 1 . GLU 19 19 53309 1 . ILE 20 20 53309 1 . LYS 21 21 53309 1 . ARG 22 22 53309 1 . ALA 23 23 53309 1 . TYR 24 24 53309 1 . ARG 25 25 53309 1 . ARG 26 26 53309 1 . GLN 27 27 53309 1 . ALA 28 28 53309 1 . LEU 29 29 53309 1 . ARG 30 30 53309 1 . TYR 31 31 53309 1 . HIS 32 32 53309 1 . PRO 33 33 53309 1 . ASP 34 34 53309 1 . LYS 35 35 53309 1 . ASN 36 36 53309 1 . LYS 37 37 53309 1 . GLU 38 38 53309 1 . PRO 39 39 53309 1 . GLY 40 40 53309 1 . ALA 41 41 53309 1 . GLU 42 42 53309 1 . GLU 43 43 53309 1 . LYS 44 44 53309 1 . PHE 45 45 53309 1 . LYS 46 46 53309 1 . GLU 47 47 53309 1 . ILE 48 48 53309 1 . ALA 49 49 53309 1 . GLU 50 50 53309 1 . ALA 51 51 53309 1 . TYR 52 52 53309 1 . ASP 53 53 53309 1 . VAL 54 54 53309 1 . LEU 55 55 53309 1 . SER 56 56 53309 1 . ASP 57 57 53309 1 . PRO 58 58 53309 1 . ARG 59 59 53309 1 . LYS 60 60 53309 1 . ARG 61 61 53309 1 . GLU 62 62 53309 1 . ILE 63 63 53309 1 . PHE 64 64 53309 1 . ASP 65 65 53309 1 . ARG 66 66 53309 1 . TYR 67 67 53309 1 . GLY 68 68 53309 1 . GLU 69 69 53309 1 . GLU 70 70 53309 1 . GLY 71 71 53309 1 . LEU 72 72 53309 1 . LYS 73 73 53309 1 . GLY 74 74 53309 1 . SER 75 75 53309 1 . GLY 76 76 53309 1 . CYS 77 77 53309 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 53309 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 53309 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 53309 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pCA528 . . . 53309 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 53309 _Sample.ID 1 _Sample.Name 'DNAJB1 J-domain Y5D Y6D' _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'DNAJB1 J-domain Y5D Y6D' '[U-100% 15N]' . . 1 $entity_1 . . 0.5 . . mM . . . . 53309 1 2 MES 'natural abundance' . . . . . . 25 . . mM . . . . 53309 1 3 'potassium chloride' 'natural abundance' . . . . . . 150 . . mM . . . . 53309 1 4 'magnesium chloride' 'natural abundance' . . . . . . 10 . . mM . . . . 53309 1 5 DTT 'natural abundance' . . . . . . 5 . . mM . . . . 53309 1 6 DSS 'natural abundance' . . . . . . 0.5 . . mM . . . . 53309 1 7 D2O 'natural abundance' . . . . . . 10 . . % . . . . 53309 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 53309 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'DNAJB1 J-domain' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 150 . mM 53309 1 pH 6.5 . pH 53309 1 pressure 1 . atm 53309 1 temperature 298 . K 53309 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 53309 _Software.ID 1 _Software.Type . _Software.Name CcpNMR _Software.Version 2.5.2 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 53309 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 53309 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name '700 MHz Bruker' _NMR_spectrometer.Details 'cryo probe' _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE III' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 700 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 53309 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53309 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 53309 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'amide backbone assignment of DNAJB1 J-domain Y5D Y6D' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 DSS 'methyl protons' . . . . ppm 0.0 internal direct 1 . . . . . 53309 1 N 15 DSS 'methyl protons' . . . . ppm 0.0 internal indirect . . . . . . 53309 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 53309 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'DNAJB1 Y5D Y6D' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N HSQC' . . . 53309 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 53309 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 1 1 MET H H 1 8.235 . . 1 . . . . . 1 MET H . 53309 1 2 . 1 . 1 1 1 MET N N 15 121.019 . . 1 . . . . . 1 MET N . 53309 1 3 . 1 . 1 2 2 GLY H H 1 8.41 . . 1 . . . . . 2 GLY H . 53309 1 4 . 1 . 1 2 2 GLY N N 15 110.905 . . 1 . . . . . 2 GLY N . 53309 1 5 . 1 . 1 3 3 LYS H H 1 8.409 . . 1 . . . . . 3 LYS H . 53309 1 6 . 1 . 1 3 3 LYS N N 15 122.183 . . 1 . . . . . 3 LYS N . 53309 1 7 . 1 . 1 4 4 ASP H H 1 8.342 . . 1 . . . . . 4 ASP H . 53309 1 8 . 1 . 1 4 4 ASP N N 15 121.201 . . 1 . . . . . 4 ASP N . 53309 1 9 . 1 . 1 5 5 ASP H H 1 8.063 . . 1 . . . . . 5 ASP H . 53309 1 10 . 1 . 1 5 5 ASP N N 15 120.724 . . 1 . . . . . 5 ASP N . 53309 1 11 . 1 . 1 6 6 ASP H H 1 8.182 . . 1 . . . . . 6 ASP H . 53309 1 12 . 1 . 1 6 6 ASP N N 15 121.047 . . 1 . . . . . 6 ASP N . 53309 1 13 . 1 . 1 7 7 GLN H H 1 8.39 . . 1 . . . . . 7 GLN H . 53309 1 14 . 1 . 1 7 7 GLN N N 15 121.496 . . 1 . . . . . 7 GLN N . 53309 1 15 . 1 . 1 8 8 THR H H 1 8.201 . . 1 . . . . . 8 THR H . 53309 1 16 . 1 . 1 8 8 THR N N 15 115.189 . . 1 . . . . . 8 THR N . 53309 1 17 . 1 . 1 9 9 LEU H H 1 8.062 . . 1 . . . . . 9 LEU H . 53309 1 18 . 1 . 1 9 9 LEU N N 15 124.017 . . 1 . . . . . 9 LEU N . 53309 1 19 . 1 . 1 10 10 GLY H H 1 8.208 . . 1 . . . . . 10 GLY H . 53309 1 20 . 1 . 1 10 10 GLY N N 15 109.321 . . 1 . . . . . 10 GLY N . 53309 1 21 . 1 . 1 11 11 LEU H H 1 7.87 . . 1 . . . . . 11 LEU H . 53309 1 22 . 1 . 1 11 11 LEU N N 15 121.909 . . 1 . . . . . 11 LEU N . 53309 1 23 . 1 . 1 12 12 ALA H H 1 8.217 . . 1 . . . . . 12 ALA H . 53309 1 24 . 1 . 1 12 12 ALA N N 15 125.116 . . 1 . . . . . 12 ALA N . 53309 1 25 . 1 . 1 13 13 ARG H H 1 8.242 . . 1 . . . . . 13 ARG H . 53309 1 26 . 1 . 1 13 13 ARG N N 15 121.032 . . 1 . . . . . 13 ARG N . 53309 1 27 . 1 . 1 14 14 GLY H H 1 8.413 . . 1 . . . . . 14 GLY H . 53309 1 28 . 1 . 1 14 14 GLY N N 15 110.928 . . 1 . . . . . 14 GLY N . 53309 1 29 . 1 . 1 15 15 ALA H H 1 7.99 . . 1 . . . . . 15 ALA H . 53309 1 30 . 1 . 1 15 15 ALA N N 15 124.133 . . 1 . . . . . 15 ALA N . 53309 1 31 . 1 . 1 16 16 SER H H 1 8.454 . . 1 . . . . . 16 SER H . 53309 1 32 . 1 . 1 16 16 SER N N 15 116.563 . . 1 . . . . . 16 SER N . 53309 1 33 . 1 . 1 17 17 ASP H H 1 8.527 . . 1 . . . . . 17 ASP H . 53309 1 34 . 1 . 1 17 17 ASP N N 15 122.419 . . 1 . . . . . 17 ASP N . 53309 1 35 . 1 . 1 18 18 GLU H H 1 8.333 . . 1 . . . . . 18 GLU H . 53309 1 36 . 1 . 1 18 18 GLU N N 15 120.14 . . 1 . . . . . 18 GLU N . 53309 1 37 . 1 . 1 19 19 GLU H H 1 7.989 . . 1 . . . . . 19 GLU H . 53309 1 38 . 1 . 1 19 19 GLU N N 15 121.221 . . 1 . . . . . 19 GLU N . 53309 1 39 . 1 . 1 20 20 ILE H H 1 8.047 . . 1 . . . . . 20 ILE H . 53309 1 40 . 1 . 1 20 20 ILE N N 15 122.24 . . 1 . . . . . 20 ILE N . 53309 1 41 . 1 . 1 21 21 LYS H H 1 7.977 . . 1 . . . . . 21 LYS H . 53309 1 42 . 1 . 1 21 21 LYS N N 15 121.498 . . 1 . . . . . 21 LYS N . 53309 1 43 . 1 . 1 22 22 ARG H H 1 7.985 . . 1 . . . . . 22 ARG H . 53309 1 44 . 1 . 1 22 22 ARG N N 15 121.35 . . 1 . . . . . 22 ARG N . 53309 1 45 . 1 . 1 23 23 ALA H H 1 7.961 . . 1 . . . . . 23 ALA H . 53309 1 46 . 1 . 1 23 23 ALA N N 15 123.336 . . 1 . . . . . 23 ALA N . 53309 1 47 . 1 . 1 24 24 TYR H H 1 8.044 . . 1 . . . . . 24 TYR H . 53309 1 48 . 1 . 1 24 24 TYR N N 15 119.465 . . 1 . . . . . 24 TYR N . 53309 1 49 . 1 . 1 25 25 ARG H H 1 7.982 . . 1 . . . . . 25 ARG H . 53309 1 50 . 1 . 1 25 25 ARG N N 15 121.488 . . 1 . . . . . 25 ARG N . 53309 1 51 . 1 . 1 26 26 ARG H H 1 7.993 . . 1 . . . . . 26 ARG H . 53309 1 52 . 1 . 1 26 26 ARG N N 15 120.373 . . 1 . . . . . 26 ARG N . 53309 1 53 . 1 . 1 27 27 GLN H H 1 8.005 . . 1 . . . . . 27 GLN H . 53309 1 54 . 1 . 1 27 27 GLN N N 15 120.303 . . 1 . . . . . 27 GLN N . 53309 1 55 . 1 . 1 28 28 ALA H H 1 8.063 . . 1 . . . . . 28 ALA H . 53309 1 56 . 1 . 1 28 28 ALA N N 15 124.347 . . 1 . . . . . 28 ALA N . 53309 1 57 . 1 . 1 29 29 LEU H H 1 7.836 . . 1 . . . . . 29 LEU H . 53309 1 58 . 1 . 1 29 29 LEU N N 15 120.133 . . 1 . . . . . 29 LEU N . 53309 1 59 . 1 . 1 30 30 ARG H H 1 7.869 . . 1 . . . . . 30 ARG H . 53309 1 60 . 1 . 1 30 30 ARG N N 15 120.599 . . 1 . . . . . 30 ARG N . 53309 1 61 . 1 . 1 31 31 TYR H H 1 8.384 . . 1 . . . . . 31 TYR H . 53309 1 62 . 1 . 1 31 31 TYR N N 15 119.573 . . 1 . . . . . 31 TYR N . 53309 1 63 . 1 . 1 32 32 HIS H H 1 8.055 . . 1 . . . . . 32 HIS H . 53309 1 64 . 1 . 1 32 32 HIS N N 15 121.721 . . 1 . . . . . 32 HIS N . 53309 1 65 . 1 . 1 34 34 ASP H H 1 8.604 . . 1 . . . . . 34 ASP H . 53309 1 66 . 1 . 1 34 34 ASP N N 15 120.913 . . 1 . . . . . 34 ASP N . 53309 1 67 . 1 . 1 35 35 LYS H H 1 7.901 . . 1 . . . . . 35 LYS H . 53309 1 68 . 1 . 1 35 35 LYS N N 15 120.84 . . 1 . . . . . 35 LYS N . 53309 1 69 . 1 . 1 36 36 ASN H H 1 7.949 . . 1 . . . . . 36 ASN H . 53309 1 70 . 1 . 1 36 36 ASN N N 15 120.923 . . 1 . . . . . 36 ASN N . 53309 1 71 . 1 . 1 37 37 LYS H H 1 8.081 . . 1 . . . . . 37 LYS H . 53309 1 72 . 1 . 1 37 37 LYS N N 15 123.214 . . 1 . . . . . 37 LYS N . 53309 1 73 . 1 . 1 38 38 GLU H H 1 8.312 . . 1 . . . . . 38 GLU H . 53309 1 74 . 1 . 1 38 38 GLU N N 15 124.167 . . 1 . . . . . 38 GLU N . 53309 1 75 . 1 . 1 40 40 GLY H H 1 8.598 . . 1 . . . . . 40 GLY H . 53309 1 76 . 1 . 1 40 40 GLY N N 15 110.705 . . 1 . . . . . 40 GLY N . 53309 1 77 . 1 . 1 41 41 ALA H H 1 8.061 . . 1 . . . . . 41 ALA H . 53309 1 78 . 1 . 1 41 41 ALA N N 15 124.262 . . 1 . . . . . 41 ALA N . 53309 1 79 . 1 . 1 42 42 GLU H H 1 8.544 . . 1 . . . . . 42 GLU H . 53309 1 80 . 1 . 1 42 42 GLU N N 15 119.877 . . 1 . . . . . 42 GLU N . 53309 1 81 . 1 . 1 43 43 GLU H H 1 8.259 . . 1 . . . . . 43 GLU H . 53309 1 82 . 1 . 1 43 43 GLU N N 15 122.25 . . 1 . . . . . 43 GLU N . 53309 1 83 . 1 . 1 44 44 LYS H H 1 8.063 . . 1 . . . . . 44 LYS H . 53309 1 84 . 1 . 1 44 44 LYS N N 15 122.079 . . 1 . . . . . 44 LYS N . 53309 1 85 . 1 . 1 45 45 PHE H H 1 8.091 . . 1 . . . . . 45 PHE H . 53309 1 86 . 1 . 1 45 45 PHE N N 15 120.752 . . 1 . . . . . 45 PHE N . 53309 1 87 . 1 . 1 46 46 LYS H H 1 7.947 . . 1 . . . . . 46 LYS H . 53309 1 88 . 1 . 1 46 46 LYS N N 15 123.146 . . 1 . . . . . 46 LYS N . 53309 1 89 . 1 . 1 47 47 GLU H H 1 8.201 . . 1 . . . . . 47 GLU H . 53309 1 90 . 1 . 1 47 47 GLU N N 15 122.168 . . 1 . . . . . 47 GLU N . 53309 1 91 . 1 . 1 48 48 ILE H H 1 8.041 . . 1 . . . . . 48 ILE H . 53309 1 92 . 1 . 1 48 48 ILE N N 15 122.21 . . 1 . . . . . 48 ILE N . 53309 1 93 . 1 . 1 49 49 ALA H H 1 8.226 . . 1 . . . . . 49 ALA H . 53309 1 94 . 1 . 1 49 49 ALA N N 15 127.783 . . 1 . . . . . 49 ALA N . 53309 1 95 . 1 . 1 50 50 GLU H H 1 8.2 . . 1 . . . . . 50 GLU H . 53309 1 96 . 1 . 1 50 50 GLU N N 15 120.293 . . 1 . . . . . 50 GLU N . 53309 1 97 . 1 . 1 51 51 ALA H H 1 8.064 . . 1 . . . . . 51 ALA H . 53309 1 98 . 1 . 1 51 51 ALA N N 15 124.661 . . 1 . . . . . 51 ALA N . 53309 1 99 . 1 . 1 52 52 TYR H H 1 7.967 . . 1 . . . . . 52 TYR H . 53309 1 100 . 1 . 1 52 52 TYR N N 15 119.627 . . 1 . . . . . 52 TYR N . 53309 1 101 . 1 . 1 53 53 ASP H H 1 8.145 . . 1 . . . . . 53 ASP H . 53309 1 102 . 1 . 1 53 53 ASP N N 15 122.671 . . 1 . . . . . 53 ASP N . 53309 1 103 . 1 . 1 54 54 VAL H H 1 7.923 . . 1 . . . . . 54 VAL H . 53309 1 104 . 1 . 1 54 54 VAL N N 15 120.827 . . 1 . . . . . 54 VAL N . 53309 1 105 . 1 . 1 55 55 LEU H H 1 8.052 . . 1 . . . . . 55 LEU H . 53309 1 106 . 1 . 1 55 55 LEU N N 15 122.988 . . 1 . . . . . 55 LEU N . 53309 1 107 . 1 . 1 56 56 SER H H 1 7.905 . . 1 . . . . . 56 SER H . 53309 1 108 . 1 . 1 56 56 SER N N 15 115.861 . . 1 . . . . . 56 SER N . 53309 1 109 . 1 . 1 57 57 ASP H H 1 7.974 . . 1 . . . . . 57 ASP H . 53309 1 110 . 1 . 1 57 57 ASP N N 15 123.602 . . 1 . . . . . 57 ASP N . 53309 1 111 . 1 . 1 59 59 ARG H H 1 8.217 . . 1 . . . . . 59 ARG H . 53309 1 112 . 1 . 1 59 59 ARG N N 15 119.226 . . 1 . . . . . 59 ARG N . 53309 1 113 . 1 . 1 61 61 ARG H H 1 7.983 . . 1 . . . . . 61 ARG H . 53309 1 114 . 1 . 1 61 61 ARG N N 15 121.308 . . 1 . . . . . 61 ARG N . 53309 1 115 . 1 . 1 62 62 GLU H H 1 8.168 . . 1 . . . . . 62 GLU H . 53309 1 116 . 1 . 1 62 62 GLU N N 15 121.401 . . 1 . . . . . 62 GLU N . 53309 1 117 . 1 . 1 63 63 ILE H H 1 7.854 . . 1 . . . . . 63 ILE H . 53309 1 118 . 1 . 1 63 63 ILE N N 15 120.791 . . 1 . . . . . 63 ILE N . 53309 1 119 . 1 . 1 64 64 PHE H H 1 8.09 . . 1 . . . . . 64 PHE H . 53309 1 120 . 1 . 1 64 64 PHE N N 15 123.345 . . 1 . . . . . 64 PHE N . 53309 1 121 . 1 . 1 65 65 ASP H H 1 8.263 . . 1 . . . . . 65 ASP H . 53309 1 122 . 1 . 1 65 65 ASP N N 15 122.448 . . 1 . . . . . 65 ASP N . 53309 1 123 . 1 . 1 66 66 ARG H H 1 7.909 . . 1 . . . . . 66 ARG H . 53309 1 124 . 1 . 1 66 66 ARG N N 15 120.883 . . 1 . . . . . 66 ARG N . 53309 1 125 . 1 . 1 67 67 TYR H H 1 8.181 . . 1 . . . . . 67 TYR H . 53309 1 126 . 1 . 1 67 67 TYR N N 15 120.029 . . 1 . . . . . 67 TYR N . 53309 1 127 . 1 . 1 68 68 GLY H H 1 8.163 . . 1 . . . . . 68 GLY H . 53309 1 128 . 1 . 1 68 68 GLY N N 15 110.842 . . 1 . . . . . 68 GLY N . 53309 1 129 . 1 . 1 69 69 GLU H H 1 8.266 . . 1 . . . . . 69 GLU H . 53309 1 130 . 1 . 1 69 69 GLU N N 15 121.165 . . 1 . . . . . 69 GLU N . 53309 1 131 . 1 . 1 70 70 GLU H H 1 8.564 . . 1 . . . . . 70 GLU H . 53309 1 132 . 1 . 1 70 70 GLU N N 15 121.538 . . 1 . . . . . 70 GLU N . 53309 1 133 . 1 . 1 71 71 GLY H H 1 8.191 . . 1 . . . . . 71 GLY H . 53309 1 134 . 1 . 1 71 71 GLY N N 15 109.291 . . 1 . . . . . 71 GLY N . 53309 1 135 . 1 . 1 72 72 LEU H H 1 7.924 . . 1 . . . . . 72 LEU H . 53309 1 136 . 1 . 1 72 72 LEU N N 15 121.857 . . 1 . . . . . 72 LEU N . 53309 1 137 . 1 . 1 73 73 LYS H H 1 8.179 . . 1 . . . . . 73 LYS H . 53309 1 138 . 1 . 1 73 73 LYS N N 15 122.34 . . 1 . . . . . 73 LYS N . 53309 1 139 . 1 . 1 74 74 GLY H H 1 8.287 . . 1 . . . . . 74 GLY H . 53309 1 140 . 1 . 1 74 74 GLY N N 15 110.452 . . 1 . . . . . 74 GLY N . 53309 1 141 . 1 . 1 75 75 SER H H 1 8.235 . . 1 . . . . . 75 SER H . 53309 1 142 . 1 . 1 75 75 SER N N 15 116.087 . . 1 . . . . . 75 SER N . 53309 1 143 . 1 . 1 76 76 GLY H H 1 8.491 . . 1 . . . . . 76 GLY H . 53309 1 144 . 1 . 1 76 76 GLY N N 15 111.978 . . 1 . . . . . 76 GLY N . 53309 1 145 . 1 . 1 77 77 CYS H H 1 7.797 . . 1 . . . . . 77 CYS H . 53309 1 146 . 1 . 1 77 77 CYS N N 15 123.274 . . 1 . . . . . 77 CYS N . 53309 1 stop_ save_