data_52705 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52705 _Entry.Title ; FOXO4(86-211) apo form ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2024-11-18 _Entry.Accession_date 2024-11-18 _Entry.Last_release_date 2024-11-19 _Entry.Original_release_date 2024-11-19 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Klara Kohoutova . . . . 52705 2 Pavel Srb . . . . 52705 3 Veronika Obsilova . . . . 52705 4 Vaclav Veverka . . . . 52705 5 Tomas Obsil . . . . 52705 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID heteronucl_T1_relaxation 1 52705 heteronucl_T2_relaxation 1 52705 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'T1 relaxation values' 76 52705 'T2 relaxation values' 76 52705 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2024-12-19 . original BMRB . 52705 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 52703 'p53(1-93) apo form' 52705 BMRB 52704 'p53-foxo4 complex' 52705 BMRB 52706 'p53-foxo4 complex' 52705 BMRB 52707 'p53-foxo4_DNA complex' 52705 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52705 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID . _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Structural Plasticity of the FOXO-DBD:p53-TAD Interaction ; _Citation.Status submitted _Citation.Type journal _Citation.Journal_abbrev 'Nat. Commun.' _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Klara Kohoutova . . . . 52705 1 2 Pavel Srb . . . . 52705 1 3 Veronika Obsilova . . . . 52705 1 4 Vaclav Veverka . . . . 52705 1 5 Tomas Obsil . . . . 52705 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52705 _Assembly.ID 1 _Assembly.Name FOXO4(86-211) _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states yes _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 FOXO4(86-211) 1 $entity_1 . . yes native yes no . . . 52705 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52705 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GAVTGPRKGGSRRNAWGNQS YAELISQAIESAPEKRLTLA QIYEWMVRTVPYFKDKGDSN SSAGWKNSIRHNLSLHSKFI KVHNEATGKSSWWMLNPEGG KSGKAPRRRAASMDSSSKLL RGRSKA ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 126 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 86 GLY . 52705 1 2 87 ALA . 52705 1 3 88 VAL . 52705 1 4 89 THR . 52705 1 5 90 GLY . 52705 1 6 91 PRO . 52705 1 7 92 ARG . 52705 1 8 93 LYS . 52705 1 9 94 GLY . 52705 1 10 95 GLY . 52705 1 11 96 SER . 52705 1 12 97 ARG . 52705 1 13 98 ARG . 52705 1 14 99 ASN . 52705 1 15 100 ALA . 52705 1 16 101 TRP . 52705 1 17 102 GLY . 52705 1 18 103 ASN . 52705 1 19 104 GLN . 52705 1 20 105 SER . 52705 1 21 106 TYR . 52705 1 22 107 ALA . 52705 1 23 108 GLU . 52705 1 24 109 LEU . 52705 1 25 110 ILE . 52705 1 26 111 SER . 52705 1 27 112 GLN . 52705 1 28 113 ALA . 52705 1 29 114 ILE . 52705 1 30 115 GLU . 52705 1 31 116 SER . 52705 1 32 117 ALA . 52705 1 33 118 PRO . 52705 1 34 119 GLU . 52705 1 35 120 LYS . 52705 1 36 121 ARG . 52705 1 37 122 LEU . 52705 1 38 123 THR . 52705 1 39 124 LEU . 52705 1 40 125 ALA . 52705 1 41 126 GLN . 52705 1 42 127 ILE . 52705 1 43 128 TYR . 52705 1 44 129 GLU . 52705 1 45 130 TRP . 52705 1 46 131 MET . 52705 1 47 132 VAL . 52705 1 48 133 ARG . 52705 1 49 134 THR . 52705 1 50 135 VAL . 52705 1 51 136 PRO . 52705 1 52 137 TYR . 52705 1 53 138 PHE . 52705 1 54 139 LYS . 52705 1 55 140 ASP . 52705 1 56 141 LYS . 52705 1 57 142 GLY . 52705 1 58 143 ASP . 52705 1 59 144 SER . 52705 1 60 145 ASN . 52705 1 61 146 SER . 52705 1 62 147 SER . 52705 1 63 148 ALA . 52705 1 64 149 GLY . 52705 1 65 150 TRP . 52705 1 66 151 LYS . 52705 1 67 152 ASN . 52705 1 68 153 SER . 52705 1 69 154 ILE . 52705 1 70 155 ARG . 52705 1 71 156 HIS . 52705 1 72 157 ASN . 52705 1 73 158 LEU . 52705 1 74 159 SER . 52705 1 75 160 LEU . 52705 1 76 161 HIS . 52705 1 77 162 SER . 52705 1 78 163 LYS . 52705 1 79 164 PHE . 52705 1 80 165 ILE . 52705 1 81 166 LYS . 52705 1 82 167 VAL . 52705 1 83 168 HIS . 52705 1 84 169 ASN . 52705 1 85 170 GLU . 52705 1 86 171 ALA . 52705 1 87 172 THR . 52705 1 88 173 GLY . 52705 1 89 174 LYS . 52705 1 90 175 SER . 52705 1 91 176 SER . 52705 1 92 177 TRP . 52705 1 93 178 TRP . 52705 1 94 179 MET . 52705 1 95 180 LEU . 52705 1 96 181 ASN . 52705 1 97 182 PRO . 52705 1 98 183 GLU . 52705 1 99 184 GLY . 52705 1 100 185 GLY . 52705 1 101 186 LYS . 52705 1 102 187 SER . 52705 1 103 188 GLY . 52705 1 104 189 LYS . 52705 1 105 190 ALA . 52705 1 106 191 PRO . 52705 1 107 192 ARG . 52705 1 108 193 ARG . 52705 1 109 194 ARG . 52705 1 110 195 ALA . 52705 1 111 196 ALA . 52705 1 112 197 SER . 52705 1 113 198 MET . 52705 1 114 199 ASP . 52705 1 115 200 SER . 52705 1 116 201 SER . 52705 1 117 202 SER . 52705 1 118 203 LYS . 52705 1 119 204 LEU . 52705 1 120 205 LEU . 52705 1 121 206 ARG . 52705 1 122 207 GLY . 52705 1 123 208 ARG . 52705 1 124 209 SER . 52705 1 125 210 LYS . 52705 1 126 211 ALA . 52705 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 52705 1 . ALA 2 2 52705 1 . VAL 3 3 52705 1 . THR 4 4 52705 1 . GLY 5 5 52705 1 . PRO 6 6 52705 1 . ARG 7 7 52705 1 . LYS 8 8 52705 1 . GLY 9 9 52705 1 . GLY 10 10 52705 1 . SER 11 11 52705 1 . ARG 12 12 52705 1 . ARG 13 13 52705 1 . ASN 14 14 52705 1 . ALA 15 15 52705 1 . TRP 16 16 52705 1 . GLY 17 17 52705 1 . ASN 18 18 52705 1 . GLN 19 19 52705 1 . SER 20 20 52705 1 . TYR 21 21 52705 1 . ALA 22 22 52705 1 . GLU 23 23 52705 1 . LEU 24 24 52705 1 . ILE 25 25 52705 1 . SER 26 26 52705 1 . GLN 27 27 52705 1 . ALA 28 28 52705 1 . ILE 29 29 52705 1 . GLU 30 30 52705 1 . SER 31 31 52705 1 . ALA 32 32 52705 1 . PRO 33 33 52705 1 . GLU 34 34 52705 1 . LYS 35 35 52705 1 . ARG 36 36 52705 1 . LEU 37 37 52705 1 . THR 38 38 52705 1 . LEU 39 39 52705 1 . ALA 40 40 52705 1 . GLN 41 41 52705 1 . ILE 42 42 52705 1 . TYR 43 43 52705 1 . GLU 44 44 52705 1 . TRP 45 45 52705 1 . MET 46 46 52705 1 . VAL 47 47 52705 1 . ARG 48 48 52705 1 . THR 49 49 52705 1 . VAL 50 50 52705 1 . PRO 51 51 52705 1 . TYR 52 52 52705 1 . PHE 53 53 52705 1 . LYS 54 54 52705 1 . ASP 55 55 52705 1 . LYS 56 56 52705 1 . GLY 57 57 52705 1 . ASP 58 58 52705 1 . SER 59 59 52705 1 . ASN 60 60 52705 1 . SER 61 61 52705 1 . SER 62 62 52705 1 . ALA 63 63 52705 1 . GLY 64 64 52705 1 . TRP 65 65 52705 1 . LYS 66 66 52705 1 . ASN 67 67 52705 1 . SER 68 68 52705 1 . ILE 69 69 52705 1 . ARG 70 70 52705 1 . HIS 71 71 52705 1 . ASN 72 72 52705 1 . LEU 73 73 52705 1 . SER 74 74 52705 1 . LEU 75 75 52705 1 . HIS 76 76 52705 1 . SER 77 77 52705 1 . LYS 78 78 52705 1 . PHE 79 79 52705 1 . ILE 80 80 52705 1 . LYS 81 81 52705 1 . VAL 82 82 52705 1 . HIS 83 83 52705 1 . ASN 84 84 52705 1 . GLU 85 85 52705 1 . ALA 86 86 52705 1 . THR 87 87 52705 1 . GLY 88 88 52705 1 . LYS 89 89 52705 1 . SER 90 90 52705 1 . SER 91 91 52705 1 . TRP 92 92 52705 1 . TRP 93 93 52705 1 . MET 94 94 52705 1 . LEU 95 95 52705 1 . ASN 96 96 52705 1 . PRO 97 97 52705 1 . GLU 98 98 52705 1 . GLY 99 99 52705 1 . GLY 100 100 52705 1 . LYS 101 101 52705 1 . SER 102 102 52705 1 . GLY 103 103 52705 1 . LYS 104 104 52705 1 . ALA 105 105 52705 1 . PRO 106 106 52705 1 . ARG 107 107 52705 1 . ARG 108 108 52705 1 . ARG 109 109 52705 1 . ALA 110 110 52705 1 . ALA 111 111 52705 1 . SER 112 112 52705 1 . MET 113 113 52705 1 . ASP 114 114 52705 1 . SER 115 115 52705 1 . SER 116 116 52705 1 . SER 117 117 52705 1 . LYS 118 118 52705 1 . LEU 119 119 52705 1 . LEU 120 120 52705 1 . ARG 121 121 52705 1 . GLY 122 122 52705 1 . ARG 123 123 52705 1 . SER 124 124 52705 1 . LYS 125 125 52705 1 . ALA 126 126 52705 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52705 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52705 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52705 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET-15b . . . 52705 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52705 _Sample.ID 1 _Sample.Name sample3_foxo4 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 FOXO4(86-211) '[U-100% 15N]' . . 1 $entity_1 . . 100 . . uM . . . . 52705 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52705 _Sample_condition_list.ID 1 _Sample_condition_list.Name sample1_conditions _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 150 . mM 52705 1 pH 6.5 . pH 52705 1 pressure 1 . atm 52705 1 temperature 298 . K 52705 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52705 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version 3.6.2 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 52705 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 52705 _Software.ID 2 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version 3.1 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'data analysis' . 52705 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52705 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name NMR850 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE III' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 850 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52705 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 'T1/R1 relaxation' no no yes . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52705 1 2 'T2/R2 relaxation' no no yes . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52705 1 stop_ save_ ######################################## # Heteronuclear T1 relaxation values # ######################################## save_heteronucl_T1_relaxation_1 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronucl_T1_relaxation_1 _Heteronucl_T1_list.Entry_ID 52705 _Heteronucl_T1_list.ID 1 _Heteronucl_T1_list.Name foxo4_free _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 850.3 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details . _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 1 'T1/R1 relaxation' . . . 52705 1 stop_ loop_ _Heteronucl_T1_software.Software_ID _Heteronucl_T1_software.Software_label _Heteronucl_T1_software.Method_ID _Heteronucl_T1_software.Method_label _Heteronucl_T1_software.Entry_ID _Heteronucl_T1_software.Heteronucl_T1_list_ID 1 $software_1 . . 52705 1 2 $software_2 . . 52705 1 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 1 1 GLY N N 15 1.78285 0.0667496 . . 86 GLY N 52705 1 2 . 1 1 2 2 ALA N N 15 1.07078 0.0218994 . . 87 ALA N 52705 1 3 . 1 1 3 3 VAL N N 15 1.74917 0.0911759 . . 88 VAL N 52705 1 4 . 1 1 4 4 THR N N 15 1.56177 0.0395137 . . 89 THR N 52705 1 5 . 1 1 5 5 GLY N N 15 1.45012 0.045211 . . 90 GLY N 52705 1 6 . 1 1 7 7 ARG N N 15 1.65344 0.0470224 . . 92 ARG N 52705 1 7 . 1 1 8 8 LYS N N 15 1.81061 0.091137 . . 93 LYS N 52705 1 8 . 1 1 9 9 GLY N N 15 1.87652 0.170081 . . 94 GLY N 52705 1 9 . 1 1 10 10 GLY N N 15 1.87266 0.195682 . . 95 GLY N 52705 1 10 . 1 1 11 11 SER N N 15 1.96232 0.269935 . . 96 SER N 52705 1 11 . 1 1 12 12 ARG N N 15 1.81061 0.08917 . . 97 ARG N 52705 1 12 . 1 1 13 13 ARG N N 15 1.34935 0.0151485 . . 98 ARG N 52705 1 13 . 1 1 14 14 ASN N N 15 1.53563 0.10423 . . 99 ASN N 52705 1 14 . 1 1 17 17 GLY N N 15 3.96825 0.588939 . . 102 GLY N 52705 1 15 . 1 1 20 20 SER N N 15 1.82116 0.172796 . . 105 SER N 52705 1 16 . 1 1 21 21 TYR N N 15 1.80571 0.163681 . . 106 TYR N 52705 1 17 . 1 1 22 22 ALA N N 15 1.76149 0.11946 . . 107 ALA N 52705 1 18 . 1 1 23 23 GLU N N 15 1.57754 0.086604 . . 108 GLU N 52705 1 19 . 1 1 26 26 SER N N 15 1.45096 0.0863164 . . 111 SER N 52705 1 20 . 1 1 27 27 GLN N N 15 1.45645 0.0638497 . . 112 GLN N 52705 1 21 . 1 1 28 28 ALA N N 15 1.58278 0.0839239 . . 113 ALA N 52705 1 22 . 1 1 29 29 ILE N N 15 1.9478 0.40595 . . 114 ILE N 52705 1 23 . 1 1 32 32 ALA N N 15 1.66583 0.122655 . . 117 ALA N 52705 1 24 . 1 1 36 36 ARG N N 15 1.62866 0.175333 . . 121 ARG N 52705 1 25 . 1 1 37 37 LEU N N 15 1.3708 0.156153 . . 122 LEU N 52705 1 26 . 1 1 38 38 THR N N 15 1.731 0.160306 . . 123 THR N 52705 1 27 . 1 1 41 41 GLN N N 15 1.51561 0.119448 . . 126 GLN N 52705 1 28 . 1 1 42 42 ILE N N 15 1.54297 0.101897 . . 127 ILE N 52705 1 29 . 1 1 47 47 VAL N N 15 1.50966 0.136061 . . 132 VAL N 52705 1 30 . 1 1 54 54 LYS N N 15 1.4497 0.118952 . . 139 LYS N 52705 1 31 . 1 1 55 55 ASP N N 15 1.56177 0.0380503 . . 140 ASP N 52705 1 32 . 1 1 56 56 LYS N N 15 1.68719 0.10988 . . 141 LYS N 52705 1 33 . 1 1 57 57 GLY N N 15 1.89 0.150386 . . 142 GLY N 52705 1 34 . 1 1 58 58 ASP N N 15 1.57828 0.150206 . . 143 ASP N 52705 1 35 . 1 1 63 63 ALA N N 15 2.03749 0.205493 . . 148 ALA N 52705 1 36 . 1 1 64 64 GLY N N 15 2.03542 0.258932 . . 149 GLY N 52705 1 37 . 1 1 67 67 ASN N N 15 1.64555 0.108313 . . 152 ASN N 52705 1 38 . 1 1 68 68 SER N N 15 1.52975 0.0919678 . . 153 SER N 52705 1 39 . 1 1 75 75 LEU N N 15 1.6787 0.17359 . . 160 LEU N 52705 1 40 . 1 1 78 78 LYS N N 15 1.65371 0.119783 . . 163 LYS N 52705 1 41 . 1 1 80 80 ILE N N 15 1.84911 0.153181 . . 165 ILE N 52705 1 42 . 1 1 82 82 VAL N N 15 1.46263 0.129213 . . 167 VAL N 52705 1 43 . 1 1 83 83 HIS N N 15 1.69147 0.0901243 . . 168 HIS N 52705 1 44 . 1 1 85 85 GLU N N 15 2.07426 0.198347 . . 170 GLU N 52705 1 45 . 1 1 86 86 ALA N N 15 1.69808 0.0599764 . . 171 ALA N 52705 1 46 . 1 1 87 87 THR N N 15 1.66556 0.106802 . . 172 THR N 52705 1 47 . 1 1 88 88 GLY N N 15 1.93386 0.241219 . . 173 GLY N 52705 1 48 . 1 1 89 89 LYS N N 15 1.98965 0.189623 . . 174 LYS N 52705 1 49 . 1 1 92 92 TRP N N 15 1.67926 0.0989791 . . 177 TRP N 52705 1 50 . 1 1 94 94 MET N N 15 1.73822 0.150165 . . 179 MET N 52705 1 51 . 1 1 95 95 LEU N N 15 1.59719 0.142346 . . 180 LEU N 52705 1 52 . 1 1 98 98 GLU N N 15 1.41723 0.0224958 . . 183 GLU N 52705 1 53 . 1 1 99 99 GLY N N 15 1.82116 0.0922023 . . 184 GLY N 52705 1 54 . 1 1 100 100 GLY N N 15 1.77462 0.134475 . . 185 GLY N 52705 1 55 . 1 1 101 101 LYS N N 15 1.08578 0.0762755 . . 186 LYS N 52705 1 56 . 1 1 102 102 SER N N 15 1.33708 0.194868 . . 187 SER N 52705 1 57 . 1 1 103 103 GLY N N 15 1.56397 0.122544 . . 188 GLY N 52705 1 58 . 1 1 104 104 LYS N N 15 1.55812 0.0441847 . . 189 LYS N 52705 1 59 . 1 1 105 105 ALA N N 15 1.47362 0.0525518 . . 190 ALA N 52705 1 60 . 1 1 107 107 ARG N N 15 1.7001 0.0531824 . . 192 ARG N 52705 1 61 . 1 1 109 109 ARG N N 15 1.734 0.109747 . . 194 ARG N 52705 1 62 . 1 1 110 110 ALA N N 15 1.755 0.097637 . . 195 ALA N 52705 1 63 . 1 1 111 111 ALA N N 15 1.523 0.0419833 . . 196 ALA N 52705 1 64 . 1 1 112 112 SER N N 15 1.55255 0.0559218 . . 197 SER N 52705 1 65 . 1 1 113 113 MET N N 15 1.25802 0.104453 . . 198 MET N 52705 1 66 . 1 1 114 114 ASP N N 15 1.34935 0.0143474 . . 199 ASP N 52705 1 67 . 1 1 115 115 SER N N 15 1.62364 0.144201 . . 200 SER N 52705 1 68 . 1 1 116 116 SER N N 15 1.65262 0.144478 . . 201 SER N 52705 1 69 . 1 1 117 117 SER N N 15 1.64474 0.111453 . . 202 SER N 52705 1 70 . 1 1 118 118 LYS N N 15 1.49365 0.0499743 . . 203 LYS N 52705 1 71 . 1 1 119 119 LEU N N 15 1.45201 0.0870743 . . 204 LEU N 52705 1 72 . 1 1 120 120 LEU N N 15 1.27992 0.0176925 . . 205 LEU N 52705 1 73 . 1 1 121 121 ARG N N 15 1.63532 0.0976113 . . 206 ARG N 52705 1 74 . 1 1 123 123 ARG N N 15 1.9459 0.122305 . . 208 ARG N 52705 1 75 . 1 1 124 124 SER N N 15 1.64123 0.154614 . . 209 SER N 52705 1 76 . 1 1 125 125 LYS N N 15 1.81061 0.0865474 . . 210 LYS N 52705 1 stop_ save_ ######################################## # Heteronuclear T2 relaxation values # ######################################## save_heteronucl_T2_relaxation_1 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronucl_T2_relaxation_1 _Heteronucl_T2_list.Entry_ID 52705 _Heteronucl_T2_list.ID 1 _Heteronucl_T2_list.Name foxo3_freeR2 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method methanol _Heteronucl_T2_list.Temp_control_method 'single scan interleaving and temperature compensation block' _Heteronucl_T2_list.Spectrometer_frequency_1H 850.3 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details . _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 2 'T2/R2 relaxation' . . . 52705 1 stop_ loop_ _Heteronucl_T2_software.Software_ID _Heteronucl_T2_software.Software_label _Heteronucl_T2_software.Method_ID _Heteronucl_T2_software.Method_label _Heteronucl_T2_software.Entry_ID _Heteronucl_T2_software.Heteronucl_T2_list_ID 1 $software_1 . . 52705 1 2 $software_2 . . 52705 1 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 1 1 GLY N N 15 3.94011 0.166112 . . . . 86 GLY N 52705 1 2 . 1 1 2 2 ALA N N 15 3.57654 0.0538527 . . . . 87 ALA N 52705 1 3 . 1 1 3 3 VAL N N 15 4.53926 0.037501 . . . . 88 VAL N 52705 1 4 . 1 1 4 4 THR N N 15 18.2882 0.682297 . . . . 89 THR N 52705 1 5 . 1 1 5 5 GLY N N 15 3.88048 0.272552 . . . . 90 GLY N 52705 1 6 . 1 1 7 7 ARG N N 15 3.37496 0.214138 . . . . 92 ARG N 52705 1 7 . 1 1 8 8 LYS N N 15 4.65116 0.123743 . . . . 93 LYS N 52705 1 8 . 1 1 9 9 GLY N N 15 4.02739 0.455777 . . . . 94 GLY N 52705 1 9 . 1 1 10 10 GLY N N 15 3.73692 0.188522 . . . . 95 GLY N 52705 1 10 . 1 1 11 11 SER N N 15 5.35045 0.30345 . . . . 96 SER N 52705 1 11 . 1 1 12 12 ARG N N 15 3.25415 0.119661 . . . . 97 ARG N 52705 1 12 . 1 1 13 13 ARG N N 15 3.13873 0.0418695 . . . . 98 ARG N 52705 1 13 . 1 1 14 14 ASN N N 15 22.7324 0.358117 . . . . 99 ASN N 52705 1 14 . 1 1 17 17 GLY N N 15 15.7654 2.30653 . . . . 102 GLY N 52705 1 15 . 1 1 20 20 SER N N 15 21.1282 1.19636 . . . . 105 SER N 52705 1 16 . 1 1 21 21 TYR N N 15 21.8245 1.32891 . . . . 106 TYR N 52705 1 17 . 1 1 22 22 ALA N N 15 18.9 0.325419 . . . . 107 ALA N 52705 1 18 . 1 1 23 23 GLU N N 15 17.8317 0.645476 . . . . 108 GLU N 52705 1 19 . 1 1 26 26 SER N N 15 17.4398 0.523134 . . . . 111 SER N 52705 1 20 . 1 1 27 27 GLN N N 15 18.0473 0.455986 . . . . 112 GLN N 52705 1 21 . 1 1 28 28 ALA N N 15 17.0561 0.277238 . . . . 113 ALA N 52705 1 22 . 1 1 29 29 ILE N N 15 22.7324 0.331246 . . . . 114 ILE N 52705 1 23 . 1 1 32 32 ALA N N 15 25.208 0.611295 . . . . 117 ALA N 52705 1 24 . 1 1 36 36 ARG N N 15 23.8834 1.11802 . . . . 121 ARG N 52705 1 25 . 1 1 37 37 LEU N N 15 21.3447 1.27112 . . . . 122 LEU N 52705 1 26 . 1 1 38 38 THR N N 15 18.2382 1.12097 . . . . 123 THR N 52705 1 27 . 1 1 41 41 GLN N N 15 19.6928 0.7601 . . . . 126 GLN N 52705 1 28 . 1 1 42 42 ILE N N 15 18.2949 0.686143 . . . . 127 ILE N 52705 1 29 . 1 1 47 47 VAL N N 15 33.8295 2.03709 . . . . 132 VAL N 52705 1 30 . 1 1 54 54 LYS N N 15 17.0736 0.355639 . . . . 139 LYS N 52705 1 31 . 1 1 55 55 ASP N N 15 6.00962 0.29398 . . . . 140 ASP N 52705 1 32 . 1 1 56 56 LYS N N 15 16.1447 0.259347 . . . . 141 LYS N 52705 1 33 . 1 1 57 57 GLY N N 15 16.1865 0.652384 . . . . 142 GLY N 52705 1 34 . 1 1 58 58 ASP N N 15 12.9049 0.223159 . . . . 143 ASP N 52705 1 35 . 1 1 63 63 ALA N N 15 15.4967 0.660403 . . . . 148 ALA N 52705 1 36 . 1 1 64 64 GLY N N 15 18.7091 1.56813 . . . . 149 GLY N 52705 1 37 . 1 1 67 67 ASN N N 15 24.7586 0.475066 . . . . 152 ASN N 52705 1 38 . 1 1 68 68 SER N N 15 18.9825 0.25692 . . . . 153 SER N 52705 1 39 . 1 1 75 75 LEU N N 15 22.7946 1.1483 . . . . 160 LEU N 52705 1 40 . 1 1 78 78 LYS N N 15 19.3836 0.495956 . . . . 163 LYS N 52705 1 41 . 1 1 80 80 ILE N N 15 34.3997 2.21285 . . . . 165 ILE N 52705 1 42 . 1 1 82 82 VAL N N 15 24.4918 1.01374 . . . . 167 VAL N 52705 1 43 . 1 1 83 83 HIS N N 15 17.4734 0.482403 . . . . 168 HIS N 52705 1 44 . 1 1 85 85 GLU N N 15 12.1996 0.293195 . . . . 170 GLU N 52705 1 45 . 1 1 86 86 ALA N N 15 13.2573 0.228484 . . . . 171 ALA N 52705 1 46 . 1 1 87 87 THR N N 15 32.3939 1.05986 . . . . 172 THR N 52705 1 47 . 1 1 88 88 GLY N N 15 12.161 0.828185 . . . . 173 GLY N 52705 1 48 . 1 1 89 89 LYS N N 15 15.3186 0.481054 . . . . 174 LYS N 52705 1 49 . 1 1 92 92 TRP N N 15 20.5086 0.828588 . . . . 177 TRP N 52705 1 50 . 1 1 94 94 MET N N 15 20.0361 1.5014 . . . . 179 MET N 52705 1 51 . 1 1 95 95 LEU N N 15 17.2384 0.826112 . . . . 180 LEU N 52705 1 52 . 1 1 98 98 GLU N N 15 4.53926 0.0391494 . . . . 183 GLU N 52705 1 53 . 1 1 99 99 GLY N N 15 6.96379 0.0998984 . . . . 184 GLY N 52705 1 54 . 1 1 100 100 GLY N N 15 5.20833 0.0952148 . . . . 185 GLY N 52705 1 55 . 1 1 101 101 LYS N N 15 4.77099 0.148638 . . . . 186 LYS N 52705 1 56 . 1 1 102 102 SER N N 15 4.56621 0.375305 . . . . 187 SER N 52705 1 57 . 1 1 103 103 GLY N N 15 4.13052 0.542547 . . . . 188 GLY N 52705 1 58 . 1 1 104 104 LYS N N 15 3.71471 0.110807 . . . . 189 LYS N 52705 1 59 . 1 1 105 105 ALA N N 15 4.05351 0.343406 . . . . 190 ALA N 52705 1 60 . 1 1 107 107 ARG N N 15 3.73832 0.104393 . . . . 192 ARG N 52705 1 61 . 1 1 109 109 ARG N N 15 4.15973 0.102955 . . . . 194 ARG N 52705 1 62 . 1 1 110 110 ALA N N 15 3.82555 0.109469 . . . . 195 ALA N 52705 1 63 . 1 1 111 111 ALA N N 15 3.27547 0.184533 . . . . 196 ALA N 52705 1 64 . 1 1 112 112 SER N N 15 3.48918 0.182616 . . . . 197 SER N 52705 1 65 . 1 1 113 113 MET N N 15 3.48554 0.17616 . . . . 198 MET N 52705 1 66 . 1 1 114 114 ASP N N 15 3.13873 0.0429531 . . . . 199 ASP N 52705 1 67 . 1 1 115 115 SER N N 15 3.49284 0.235459 . . . . 200 SER N 52705 1 68 . 1 1 116 116 SER N N 15 3.97614 0.229241 . . . . 201 SER N 52705 1 69 . 1 1 117 117 SER N N 15 3.68324 0.244193 . . . . 202 SER N 52705 1 70 . 1 1 118 118 LYS N N 15 3.73274 0.157447 . . . . 203 LYS N 52705 1 71 . 1 1 119 119 LEU N N 15 3.20205 0.103557 . . . . 204 LEU N 52705 1 72 . 1 1 120 120 LEU N N 15 2.53421 0.0254963 . . . . 205 LEU N 52705 1 73 . 1 1 121 121 ARG N N 15 2.57798 0.155516 . . . . 206 ARG N 52705 1 74 . 1 1 123 123 ARG N N 15 4.77099 0.13794 . . . . 208 ARG N 52705 1 75 . 1 1 124 124 SER N N 15 3.97614 0.222917 . . . . 209 SER N 52705 1 76 . 1 1 125 125 LYS N N 15 2.7972 0.0618123 . . . . 210 LYS N 52705 1 stop_ save_