data_52703 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52703 _Entry.Title ; p53(1-93) apo form ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2024-11-18 _Entry.Accession_date 2024-11-18 _Entry.Last_release_date 2024-11-19 _Entry.Original_release_date 2024-11-19 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Klara Kohoutova . . . . 52703 2 Pavel Srb . . . . 52703 3 Veronika Obsilova . . . . 52703 4 Vaclav Veverka . . . . 52703 5 Tomas Obsil . . . . 52703 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID heteronucl_T1_relaxation 1 52703 heteronucl_T2_relaxation 1 52703 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'T1 relaxation values' 64 52703 'T2 relaxation values' 64 52703 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2024-12-19 . original BMRB . 52703 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 52704 'p53-foxo4 complex' 52703 BMRB 52705 'FOXO4(86-211) apo form' 52703 BMRB 52706 'p53-foxo4 complex' 52703 BMRB 52707 'p53-foxo4_DNA complex' 52703 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52703 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID . _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Structural Plasticity of the FOXO-DBD:p53-TAD Interaction ; _Citation.Status submitted _Citation.Type journal _Citation.Journal_abbrev 'Nat. Commun.' _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Klara Kohoutova . . . . 52703 1 2 Pavel Srb . . . . 52703 1 3 Veronika Obsilova . . . . 52703 1 4 Vaclav Veverka . . . . 52703 1 5 Tomas Obsil . . . . 52703 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52703 _Assembly.ID 1 _Assembly.Name p53(1-93) _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states yes _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 p53(1-93) 1 $entity_1 . . yes native yes no . . . 52703 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52703 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MEEPQSDPSVEPPLSQETFS DLWKLLPENNVLSPLPSQAM DDLMLSPDDIEQWFTEDPGP DEAPRMPEAAPPVAPAPAAP TPAAPAPAPSWPL ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states yes _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 93 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 52703 1 2 . GLU . 52703 1 3 . GLU . 52703 1 4 . PRO . 52703 1 5 . GLN . 52703 1 6 . SER . 52703 1 7 . ASP . 52703 1 8 . PRO . 52703 1 9 . SER . 52703 1 10 . VAL . 52703 1 11 . GLU . 52703 1 12 . PRO . 52703 1 13 . PRO . 52703 1 14 . LEU . 52703 1 15 . SER . 52703 1 16 . GLN . 52703 1 17 . GLU . 52703 1 18 . THR . 52703 1 19 . PHE . 52703 1 20 . SER . 52703 1 21 . ASP . 52703 1 22 . LEU . 52703 1 23 . TRP . 52703 1 24 . LYS . 52703 1 25 . LEU . 52703 1 26 . LEU . 52703 1 27 . PRO . 52703 1 28 . GLU . 52703 1 29 . ASN . 52703 1 30 . ASN . 52703 1 31 . VAL . 52703 1 32 . LEU . 52703 1 33 . SER . 52703 1 34 . PRO . 52703 1 35 . LEU . 52703 1 36 . PRO . 52703 1 37 . SER . 52703 1 38 . GLN . 52703 1 39 . ALA . 52703 1 40 . MET . 52703 1 41 . ASP . 52703 1 42 . ASP . 52703 1 43 . LEU . 52703 1 44 . MET . 52703 1 45 . LEU . 52703 1 46 . SER . 52703 1 47 . PRO . 52703 1 48 . ASP . 52703 1 49 . ASP . 52703 1 50 . ILE . 52703 1 51 . GLU . 52703 1 52 . GLN . 52703 1 53 . TRP . 52703 1 54 . PHE . 52703 1 55 . THR . 52703 1 56 . GLU . 52703 1 57 . ASP . 52703 1 58 . PRO . 52703 1 59 . GLY . 52703 1 60 . PRO . 52703 1 61 . ASP . 52703 1 62 . GLU . 52703 1 63 . ALA . 52703 1 64 . PRO . 52703 1 65 . ARG . 52703 1 66 . MET . 52703 1 67 . PRO . 52703 1 68 . GLU . 52703 1 69 . ALA . 52703 1 70 . ALA . 52703 1 71 . PRO . 52703 1 72 . PRO . 52703 1 73 . VAL . 52703 1 74 . ALA . 52703 1 75 . PRO . 52703 1 76 . ALA . 52703 1 77 . PRO . 52703 1 78 . ALA . 52703 1 79 . ALA . 52703 1 80 . PRO . 52703 1 81 . THR . 52703 1 82 . PRO . 52703 1 83 . ALA . 52703 1 84 . ALA . 52703 1 85 . PRO . 52703 1 86 . ALA . 52703 1 87 . PRO . 52703 1 88 . ALA . 52703 1 89 . PRO . 52703 1 90 . SER . 52703 1 91 . TRP . 52703 1 92 . PRO . 52703 1 93 . LEU . 52703 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 52703 1 . GLU 2 2 52703 1 . GLU 3 3 52703 1 . PRO 4 4 52703 1 . GLN 5 5 52703 1 . SER 6 6 52703 1 . ASP 7 7 52703 1 . PRO 8 8 52703 1 . SER 9 9 52703 1 . VAL 10 10 52703 1 . GLU 11 11 52703 1 . PRO 12 12 52703 1 . PRO 13 13 52703 1 . LEU 14 14 52703 1 . SER 15 15 52703 1 . GLN 16 16 52703 1 . GLU 17 17 52703 1 . THR 18 18 52703 1 . PHE 19 19 52703 1 . SER 20 20 52703 1 . ASP 21 21 52703 1 . LEU 22 22 52703 1 . TRP 23 23 52703 1 . LYS 24 24 52703 1 . LEU 25 25 52703 1 . LEU 26 26 52703 1 . PRO 27 27 52703 1 . GLU 28 28 52703 1 . ASN 29 29 52703 1 . ASN 30 30 52703 1 . VAL 31 31 52703 1 . LEU 32 32 52703 1 . SER 33 33 52703 1 . PRO 34 34 52703 1 . LEU 35 35 52703 1 . PRO 36 36 52703 1 . SER 37 37 52703 1 . GLN 38 38 52703 1 . ALA 39 39 52703 1 . MET 40 40 52703 1 . ASP 41 41 52703 1 . ASP 42 42 52703 1 . LEU 43 43 52703 1 . MET 44 44 52703 1 . LEU 45 45 52703 1 . SER 46 46 52703 1 . PRO 47 47 52703 1 . ASP 48 48 52703 1 . ASP 49 49 52703 1 . ILE 50 50 52703 1 . GLU 51 51 52703 1 . GLN 52 52 52703 1 . TRP 53 53 52703 1 . PHE 54 54 52703 1 . THR 55 55 52703 1 . GLU 56 56 52703 1 . ASP 57 57 52703 1 . PRO 58 58 52703 1 . GLY 59 59 52703 1 . PRO 60 60 52703 1 . ASP 61 61 52703 1 . GLU 62 62 52703 1 . ALA 63 63 52703 1 . PRO 64 64 52703 1 . ARG 65 65 52703 1 . MET 66 66 52703 1 . PRO 67 67 52703 1 . GLU 68 68 52703 1 . ALA 69 69 52703 1 . ALA 70 70 52703 1 . PRO 71 71 52703 1 . PRO 72 72 52703 1 . VAL 73 73 52703 1 . ALA 74 74 52703 1 . PRO 75 75 52703 1 . ALA 76 76 52703 1 . PRO 77 77 52703 1 . ALA 78 78 52703 1 . ALA 79 79 52703 1 . PRO 80 80 52703 1 . THR 81 81 52703 1 . PRO 82 82 52703 1 . ALA 83 83 52703 1 . ALA 84 84 52703 1 . PRO 85 85 52703 1 . ALA 86 86 52703 1 . PRO 87 87 52703 1 . ALA 88 88 52703 1 . PRO 89 89 52703 1 . SER 90 90 52703 1 . TRP 91 91 52703 1 . PRO 92 92 52703 1 . LEU 93 93 52703 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52703 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52703 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52703 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET21 . . . 52703 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52703 _Sample.ID 1 _Sample.Name sample1_p53free _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 p53 '[U-100% 15N]' . . 1 $entity_1 . . 100 . . uM . . . . 52703 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52703 _Sample_condition_list.ID 1 _Sample_condition_list.Name sample1_conditions _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 150 . mM 52703 1 pH 6.5 . pH 52703 1 pressure 1 . atm 52703 1 temperature 298 . K 52703 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52703 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version 3.6.2 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 52703 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 52703 _Software.ID 2 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version 3.1 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'data analysis' . 52703 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52703 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name NMR850 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE III' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 850 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52703 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 'T1/R1 relaxation' no no yes . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52703 1 2 'T2/R2 relaxation' no no yes . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52703 1 stop_ save_ ######################################## # Heteronuclear T1 relaxation values # ######################################## save_heteronucl_T1_relaxation_1 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronucl_T1_relaxation_1 _Heteronucl_T1_list.Entry_ID 52703 _Heteronucl_T1_list.ID 1 _Heteronucl_T1_list.Name p53freeR1 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 850.3 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details . _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 1 'T1/R1 relaxation' . . . 52703 1 stop_ loop_ _Heteronucl_T1_software.Software_ID _Heteronucl_T1_software.Software_label _Heteronucl_T1_software.Method_ID _Heteronucl_T1_software.Method_label _Heteronucl_T1_software.Entry_ID _Heteronucl_T1_software.Heteronucl_T1_list_ID 1 $software_1 . . 52703 1 2 $software_2 . . 52703 1 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 2 2 GLU N N 15 1.15048 0.0232956 . . . . . 52703 1 2 . 1 1 5 5 GLN N N 15 1.29066 0.0228214 . . . . . 52703 1 3 . 1 1 6 6 SER N N 15 1.20934 0.0356848 . . . . . 52703 1 4 . 1 1 7 7 ASP N N 15 1.52369 0.0172962 . . . . . 52703 1 5 . 1 1 9 9 SER N N 15 1.67842 0.029861 . . . . . 52703 1 6 . 1 1 10 10 VAL N N 15 1.56617 0.0309064 . . . . . 52703 1 7 . 1 1 11 11 GLU N N 15 1.48192 0.0204236 . . . . . 52703 1 8 . 1 1 14 14 LEU N N 15 1.38274 0.225614 . . . . . 52703 1 9 . 1 1 15 15 SER N N 15 0.878735 0.0491875 . . . . . 52703 1 10 . 1 1 16 16 GLN N N 15 1.83655 0.0664462 . . . . . 52703 1 11 . 1 1 17 17 GLU N N 15 0.521376 0.0288143 . . . . . 52703 1 12 . 1 1 18 18 THR N N 15 1.83318 0.060826 . . . . . 52703 1 13 . 1 1 19 19 PHE N N 15 1.95848 0.0786307 . . . . . 52703 1 14 . 1 1 20 20 SER N N 15 1.98177 0.108004 . . . . . 52703 1 15 . 1 1 21 21 ASP N N 15 2.02429 0.0905604 . . . . . 52703 1 16 . 1 1 22 22 LEU N N 15 1.972 0.0696091 . . . . . 52703 1 17 . 1 1 23 23 TRP N N 15 2.08594 0.0883283 . . . . . 52703 1 18 . 1 1 24 24 LYS N N 15 2.07211 0.0807204 . . . . . 52703 1 19 . 1 1 25 25 LEU N N 15 1.93199 0.0679333 . . . . . 52703 1 20 . 1 1 26 26 LEU N N 15 1.68322 0.0413651 . . . . . 52703 1 21 . 1 1 28 28 GLU N N 15 1.75346 0.0341284 . . . . . 52703 1 22 . 1 1 29 29 ASN N N 15 1.80995 0.0740362 . . . . . 52703 1 23 . 1 1 30 30 ASN N N 15 1.37193 0.0227745 . . . . . 52703 1 24 . 1 1 31 31 VAL N N 15 1.45985 0.021738 . . . . . 52703 1 25 . 1 1 32 32 LEU N N 15 1.26279 0.0158984 . . . . . 52703 1 26 . 1 1 33 33 SER N N 15 1.36351 0.0269579 . . . . . 52703 1 27 . 1 1 35 35 LEU N N 15 1.41363 0.0159068 . . . . . 52703 1 28 . 1 1 37 37 SER N N 15 1.45497 0.0436088 . . . . . 52703 1 29 . 1 1 38 38 GLN N N 15 1.53468 0.0282631 . . . . . 52703 1 30 . 1 1 39 39 ALA N N 15 1.46199 0.0184245 . . . . . 52703 1 31 . 1 1 40 40 MET N N 15 1.37193 0.0240921 . . . . . 52703 1 32 . 1 1 41 41 ASP N N 15 1.51332 0.0159164 . . . . . 52703 1 33 . 1 1 42 42 ASP N N 15 1.67616 0.0331524 . . . . . 52703 1 34 . 1 1 43 43 LEU N N 15 1.55207 0.0203313 . . . . . 52703 1 35 . 1 1 45 45 LEU N N 15 1.51035 0.0271456 . . . . . 52703 1 36 . 1 1 46 46 SER N N 15 1.4881 0.0236944 . . . . . 52703 1 37 . 1 1 48 48 ASP N N 15 1.67757 0.0346152 . . . . . 52703 1 38 . 1 1 49 49 ASP N N 15 1.5977 0.0223867 . . . . . 52703 1 39 . 1 1 50 50 ILE N N 15 1.57011 0.0271175 . . . . . 52703 1 40 . 1 1 51 51 GLU N N 15 1.72712 0.0447439 . . . . . 52703 1 41 . 1 1 52 52 GLN N N 15 1.66279 0.0411964 . . . . . 52703 1 42 . 1 1 53 53 TRP N N 15 1.7762 0.0476387 . . . . . 52703 1 43 . 1 1 55 55 THR N N 15 1.60668 0.0307191 . . . . . 52703 1 44 . 1 1 56 56 GLU N N 15 1.5977 0.0268028 . . . . . 52703 1 45 . 1 1 57 57 ASP N N 15 1.52369 0.0182017 . . . . . 52703 1 46 . 1 1 59 59 GLY N N 15 1.50852 0.0225744 . . . . . 52703 1 47 . 1 1 61 61 ASP N N 15 1.38389 0.0157617 . . . . . 52703 1 48 . 1 1 62 62 GLU N N 15 1.41203 0.0153724 . . . . . 52703 1 49 . 1 1 63 63 ALA N N 15 1.33958 0.0193805 . . . . . 52703 1 50 . 1 1 65 65 ARG N N 15 1.39528 0.0139003 . . . . . 52703 1 51 . 1 1 66 66 MET N N 15 1.41483 0.0270234 . . . . . 52703 1 52 . 1 1 68 68 GLU N N 15 1.28684 0.018381 . . . . . 52703 1 53 . 1 1 69 69 ALA N N 15 1.26279 0.015133 . . . . . 52703 1 54 . 1 1 70 70 ALA N N 15 0.993049 0.0156797 . . . . . 52703 1 55 . 1 1 73 73 VAL N N 15 1.15088 0.00568222 . . . . . 52703 1 56 . 1 1 74 74 ALA N N 15 1.10558 0.0178458 . . . . . 52703 1 57 . 1 1 79 79 ALA N N 15 0.993049 0.0170603 . . . . . 52703 1 58 . 1 1 81 81 THR N N 15 1.04167 0.0271267 . . . . . 52703 1 59 . 1 1 83 83 ALA N N 15 0.983284 0.0160497 . . . . . 52703 1 60 . 1 1 84 84 ALA N N 15 1.03638 0.0156815 . . . . . 52703 1 61 . 1 1 86 86 ALA N N 15 1.05441 0.00894979 . . . . . 52703 1 62 . 1 1 90 90 SER N N 15 0.856164 0.0546098 . . . . . 52703 1 63 . 1 1 91 91 TRP N N 15 0.987167 0.0310865 . . . . . 52703 1 64 . 1 1 93 93 LEU N N 15 0.664011 0.00965593 . . . . . 52703 1 stop_ save_ ######################################## # Heteronuclear T2 relaxation values # ######################################## save_heteronucl_T2_relaxation_1 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronucl_T2_relaxation_1 _Heteronucl_T2_list.Entry_ID 52703 _Heteronucl_T2_list.ID 1 _Heteronucl_T2_list.Name p53freeR2 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method methanol _Heteronucl_T2_list.Temp_control_method 'single scan interleaving and temperature compensation block' _Heteronucl_T2_list.Spectrometer_frequency_1H 850.3 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details . _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 2 'T2/R2 relaxation' . . . 52703 1 stop_ loop_ _Heteronucl_T2_software.Software_ID _Heteronucl_T2_software.Software_label _Heteronucl_T2_software.Method_ID _Heteronucl_T2_software.Method_label _Heteronucl_T2_software.Entry_ID _Heteronucl_T2_software.Heteronucl_T2_list_ID 1 $software_1 . . 52703 1 2 $software_2 . . 52703 1 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 2 2 GLU N N 15 2.37756 0.0638763 . . . . . . . 52703 1 2 . 1 1 5 5 GLN N N 15 2.72331 0.093447 . . . . . . . 52703 1 3 . 1 1 6 6 SER N N 15 2.80191 0.0918529 . . . . . . . 52703 1 4 . 1 1 7 7 ASP N N 15 3.54359 0.0649197 . . . . . . . 52703 1 5 . 1 1 9 9 SER N N 15 3.66569 0.0658427 . . . . . . . 52703 1 6 . 1 1 10 10 VAL N N 15 3.52237 0.0833755 . . . . . . . 52703 1 7 . 1 1 11 11 GLU N N 15 3.52113 0.0999306 . . . . . . . 52703 1 8 . 1 1 14 14 LEU N N 15 4.36872 0.597383 . . . . . . . 52703 1 9 . 1 1 15 15 SER N N 15 2.79877 0.80681 . . . . . . . 52703 1 10 . 1 1 16 16 GLN N N 15 4.7824 0.0711299 . . . . . . . 52703 1 11 . 1 1 17 17 GLU N N 15 2.65675 0.103051 . . . . . . . 52703 1 12 . 1 1 18 18 THR N N 15 4.31593 0.123126 . . . . . . . 52703 1 13 . 1 1 19 19 PHE N N 15 4.86381 0.107401 . . . . . . . 52703 1 14 . 1 1 20 20 SER N N 15 4.92126 0.225961 . . . . . . . 52703 1 15 . 1 1 21 21 ASP N N 15 5.14668 0.0958877 . . . . . . . 52703 1 16 . 1 1 22 22 LEU N N 15 5.32481 0.10179 . . . . . . . 52703 1 17 . 1 1 23 23 TRP N N 15 5.74383 0.0913865 . . . . . . . 52703 1 18 . 1 1 24 24 LYS N N 15 6.18812 0.108752 . . . . . . . 52703 1 19 . 1 1 25 25 LEU N N 15 5.71429 0.0760816 . . . . . . . 52703 1 20 . 1 1 26 26 LEU N N 15 5.09165 0.0829597 . . . . . . . 52703 1 21 . 1 1 28 28 GLU N N 15 4.81232 0.0595171 . . . . . . . 52703 1 22 . 1 1 29 29 ASN N N 15 4.60829 0.0758139 . . . . . . . 52703 1 23 . 1 1 30 30 ASN N N 15 3.98248 0.109435 . . . . . . . 52703 1 24 . 1 1 31 31 VAL N N 15 3.76506 0.0554269 . . . . . . . 52703 1 25 . 1 1 32 32 LEU N N 15 3.7037 0.0946502 . . . . . . . 52703 1 26 . 1 1 33 33 SER N N 15 3.59842 0.065261 . . . . . . . 52703 1 27 . 1 1 35 35 LEU N N 15 3.52113 0.0855485 . . . . . . . 52703 1 28 . 1 1 37 37 SER N N 15 3.73692 0.0946798 . . . . . . . 52703 1 29 . 1 1 38 38 GLN N N 15 3.89408 0.120249 . . . . . . . 52703 1 30 . 1 1 39 39 ALA N N 15 3.40948 0.105551 . . . . . . . 52703 1 31 . 1 1 40 40 MET N N 15 3.64964 0.0783206 . . . . . . . 52703 1 32 . 1 1 41 41 ASP N N 15 3.66569 0.0776675 . . . . . . . 52703 1 33 . 1 1 42 42 ASP N N 15 4.1841 0.0572469 . . . . . . . 52703 1 34 . 1 1 43 43 LEU N N 15 3.77216 0.0490907 . . . . . . . 52703 1 35 . 1 1 45 45 LEU N N 15 3.88802 0.0867701 . . . . . . . 52703 1 36 . 1 1 46 46 SER N N 15 3.7679 0.0623251 . . . . . . . 52703 1 37 . 1 1 48 48 ASP N N 15 4.73485 0.0952799 . . . . . . . 52703 1 38 . 1 1 49 49 ASP N N 15 4.01929 0.0673652 . . . . . . . 52703 1 39 . 1 1 50 50 ILE N N 15 4.43656 0.0503886 . . . . . . . 52703 1 40 . 1 1 51 51 GLU N N 15 5.68182 0.114928 . . . . . . . 52703 1 41 . 1 1 52 52 GLN N N 15 3.22165 0.045045 . . . . . . . 52703 1 42 . 1 1 53 53 TRP N N 15 4.8216 0.0623042 . . . . . . . 52703 1 43 . 1 1 55 55 THR N N 15 4.25894 0.0752752 . . . . . . . 52703 1 44 . 1 1 56 56 GLU N N 15 3.87898 0.0738781 . . . . . . . 52703 1 45 . 1 1 57 57 ASP N N 15 3.54359 0.0587668 . . . . . . . 52703 1 46 . 1 1 59 59 GLY N N 15 3.95413 0.119609 . . . . . . . 52703 1 47 . 1 1 61 61 ASP N N 15 3.72301 0.0819173 . . . . . . . 52703 1 48 . 1 1 62 62 GLU N N 15 3.16056 0.0894026 . . . . . . . 52703 1 49 . 1 1 63 63 ALA N N 15 5.42299 0.136751 . . . . . . . 52703 1 50 . 1 1 65 65 ARG N N 15 3.32336 0.0736684 . . . . . . . 52703 1 51 . 1 1 66 66 MET N N 15 3.61795 0.0905795 . . . . . . . 52703 1 52 . 1 1 68 68 GLU N N 15 3.40483 0.0438212 . . . . . . . 52703 1 53 . 1 1 69 69 ALA N N 15 2.88434 0.103993 . . . . . . . 52703 1 54 . 1 1 70 70 ALA N N 15 3.10078 0.0599002 . . . . . . . 52703 1 55 . 1 1 73 73 VAL N N 15 3.22165 0.0455639 . . . . . . . 52703 1 56 . 1 1 74 74 ALA N N 15 5.25486 0.097752 . . . . . . . 52703 1 57 . 1 1 79 79 ALA N N 15 2.73373 0.103879 . . . . . . . 52703 1 58 . 1 1 81 81 THR N N 15 2.74424 0.0670998 . . . . . . . 52703 1 59 . 1 1 83 83 ALA N N 15 2.62467 0.0819779 . . . . . . . 52703 1 60 . 1 1 84 84 ALA N N 15 2.37304 0.0771492 . . . . . . . 52703 1 61 . 1 1 86 86 ALA N N 15 2.48262 0.0733446 . . . . . . . 52703 1 62 . 1 1 90 90 SER N N 15 2.12314 0.125315 . . . . . . . 52703 1 63 . 1 1 91 91 TRP N N 15 1.9988 0.0854974 . . . . . . . 52703 1 64 . 1 1 93 93 LEU N N 15 3.43171 0.297949 . . . . . . . 52703 1 stop_ save_