data_50721 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 50721 _Entry.Title ; Phospholamban bound to SERCA in oriented bicelles (calcium-bound E1 state) ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2021-01-18 _Entry.Accession_date 2021-01-18 _Entry.Last_release_date 2021-01-20 _Entry.Original_release_date 2021-01-20 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solid-state _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Daniel Weber . K. . 0000-0001-8400-767X 50721 2 Maximo Sanz-Hernandez . . . 0000-0001-8522-8730 50721 3 'Venkateswara Reddy' Uddigiri . . . . 50721 4 Songlin Wang . . . 0000-0002-7588-7377 50721 5 Erik Larsen . K. . . 50721 6 Tata Gopinath . . . . 50721 7 Martin Gustavsson . . . 0000-0002-3015-2043 50721 8 Razvan Cornea . . . 0000-0001-5739-0992 50721 9 David Thomas . D. . . 50721 10 Alfonso 'De Simone' . . . 0000-0001-8789-9546 50721 11 Gianluigi Veglia . . . 0000-0002-2795-6964 50721 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 50721 dipolar_couplings 1 50721 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 10 50721 'dipolar coupling values' 10 50721 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2021-05-18 . original BMRB . 50721 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 50718 'Monomeric phospholamban in oriented bicelles' 50721 BMRB 50719 'Monomeric phosphorylated phospholamban in oriented bicelles' 50721 BMRB 50720 'Phospholamban bound to SERCA in oriented bicelles (calcium-free E2 state)' 50721 BMRB 50722 'Phosphorylated phospholamban bound to SERCA in oriented bicelles (calcium-free E2 state)' 50721 BMRB 50723 'Phosphorylated phospholamban bound to SERCA in oriented bicelles (calcium-bound E1 state)' 50721 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 50721 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 33978571 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Structural basis for allosteric control of the SERCA-Phospholamban membrane complex by Ca 2+ and phosphorylation ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev Elife _Citation.Journal_name_full . _Citation.Journal_volume 10 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first e66226 _Citation.Page_last e66226 _Citation.Year 2021 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Daniel Weber D. K. . . 50721 1 2 'U Venkateswara' Reddy U. V. . . 50721 1 3 Songlin Wang S. . . . 50721 1 4 Erik Larsen E. K. . . 50721 1 5 Tata Gopinath T. . . . 50721 1 6 Martin Gustavsson M. B. . . 50721 1 7 Razvan Cornea R. L. . . 50721 1 8 David Thomas D. D. . . 50721 1 9 Alfonso 'De Simone' A. . . . 50721 1 10 Gianluigi Veglia G. . . . 50721 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 50721 _Assembly.ID 1 _Assembly.Name 'Phospholamban bound to SERCA (calcium-bound E1 state)' _Assembly.BMRB_code . _Assembly.Number_of_components 3 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 1 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange yes _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass 6165.6 _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 PLN 1 $entity_1 . . yes native no no . . . 50721 1 2 SERCA 2 $entity_2 . . no native no no . . . 50721 1 3 'CA, ion' 3 $entity_CA . . no native no no . . . 50721 1 stop_ loop_ _Assembly_db_link.Author_supplied _Assembly_db_link.Database_code _Assembly_db_link.Accession_code _Assembly_db_link.Entry_mol_code _Assembly_db_link.Entry_mol_name _Assembly_db_link.Entry_experimental_method _Assembly_db_link.Entry_structure_resolution _Assembly_db_link.Entry_relation_type _Assembly_db_link.Entry_details _Assembly_db_link.Entry_ID _Assembly_db_link.Assembly_ID yes UNP P04191 . . . . . . 50721 1 yes UNP P61015 . . . . . . 50721 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 50721 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; AMEKVQYLTRSAIRRASTIE MPQQARQNLQNLFINFALIL IFLLLIAIIVMLL ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq ; A0, M1, E2, K3, V4, Q5, Y6, L7, T8, R9, S10, A11, I12, R13, R14, A15, S16, T17, I18, E19, M20, P21, Q22, Q23, A24, R25, Q26, N27, L28, Q29, N30, L31, F32, I33, N34, F35, A36, L37, I38, L39, I40, F41, L42, L43, L44, I45, A46, I47, I48, V49, M50, L51, L52 ; _Entity.Polymer_author_seq_details ; Monomeric cysteine-null mutant (C36A/C41F/C46A). N-terminal Ala0 left over from cleavage of MBP fusion protein. ; _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 53 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation 'C36A, C41F, C46A' _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 6166 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 yes UNP P61015 . . . . . . . . . . . . . . . . 50721 1 2 yes PDB 2KB7 . . . . . . . . . . . . . . . . 50721 1 3 yes PDB 2LPF . . . . . . . . . . . . . . . . 50721 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 0 ALA . 50721 1 2 1 MET . 50721 1 3 2 GLU . 50721 1 4 3 LYS . 50721 1 5 4 VAL . 50721 1 6 5 GLN . 50721 1 7 6 TYR . 50721 1 8 7 LEU . 50721 1 9 8 THR . 50721 1 10 9 ARG . 50721 1 11 10 SER . 50721 1 12 11 ALA . 50721 1 13 12 ILE . 50721 1 14 13 ARG . 50721 1 15 14 ARG . 50721 1 16 15 ALA . 50721 1 17 16 SER . 50721 1 18 17 THR . 50721 1 19 18 ILE . 50721 1 20 19 GLU . 50721 1 21 20 MET . 50721 1 22 21 PRO . 50721 1 23 22 GLN . 50721 1 24 23 GLN . 50721 1 25 24 ALA . 50721 1 26 25 ARG . 50721 1 27 26 GLN . 50721 1 28 27 ASN . 50721 1 29 28 LEU . 50721 1 30 29 GLN . 50721 1 31 30 ASN . 50721 1 32 31 LEU . 50721 1 33 32 PHE . 50721 1 34 33 ILE . 50721 1 35 34 ASN . 50721 1 36 35 PHE . 50721 1 37 36 ALA . 50721 1 38 37 LEU . 50721 1 39 38 ILE . 50721 1 40 39 LEU . 50721 1 41 40 ILE . 50721 1 42 41 PHE . 50721 1 43 42 LEU . 50721 1 44 43 LEU . 50721 1 45 44 LEU . 50721 1 46 45 ILE . 50721 1 47 46 ALA . 50721 1 48 47 ILE . 50721 1 49 48 ILE . 50721 1 50 49 VAL . 50721 1 51 50 MET . 50721 1 52 51 LEU . 50721 1 53 52 LEU . 50721 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ALA 1 1 50721 1 . MET 2 2 50721 1 . GLU 3 3 50721 1 . LYS 4 4 50721 1 . VAL 5 5 50721 1 . GLN 6 6 50721 1 . TYR 7 7 50721 1 . LEU 8 8 50721 1 . THR 9 9 50721 1 . ARG 10 10 50721 1 . SER 11 11 50721 1 . ALA 12 12 50721 1 . ILE 13 13 50721 1 . ARG 14 14 50721 1 . ARG 15 15 50721 1 . ALA 16 16 50721 1 . SER 17 17 50721 1 . THR 18 18 50721 1 . ILE 19 19 50721 1 . GLU 20 20 50721 1 . MET 21 21 50721 1 . PRO 22 22 50721 1 . GLN 23 23 50721 1 . GLN 24 24 50721 1 . ALA 25 25 50721 1 . ARG 26 26 50721 1 . GLN 27 27 50721 1 . ASN 28 28 50721 1 . LEU 29 29 50721 1 . GLN 30 30 50721 1 . ASN 31 31 50721 1 . LEU 32 32 50721 1 . PHE 33 33 50721 1 . ILE 34 34 50721 1 . ASN 35 35 50721 1 . PHE 36 36 50721 1 . ALA 37 37 50721 1 . LEU 38 38 50721 1 . ILE 39 39 50721 1 . LEU 40 40 50721 1 . ILE 41 41 50721 1 . PHE 42 42 50721 1 . LEU 43 43 50721 1 . LEU 44 44 50721 1 . LEU 45 45 50721 1 . ILE 46 46 50721 1 . ALA 47 47 50721 1 . ILE 48 48 50721 1 . ILE 49 49 50721 1 . VAL 50 50 50721 1 . MET 51 51 50721 1 . LEU 52 52 50721 1 . LEU 53 53 50721 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 50721 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name entity_2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MEAAHSKSTEECLAYFGVSE TTGLTPDQVKRHLEKYGHNE LPAEEGKSLWELVIEQFEDL LVRILLLAACISFVLAWFEE GEETITAFVEPFVILLILIA NAIVGVWQERNAENAIEALK EYEPEMGKVYRADRKSVQRI KARDIVPGDIVEVAVGDKVP ADIRILSIKSTTLRVDQSIL TGESVSVIKHTEPVPDPRAV NQDKKNMLFSGTNIAAGKAL GIVATTGVSTEIGKIRDQMA ATEQDKTPLQQKLDEFGEQL SKVISLICVAVWLINIGHFN DPVHGGSWIRGAIYYFKIAV ALAVAAIPEGLPAVITTCLA LGTRRMAKKNAIVRSLPSVE TLGCTSVICSDKTGTLTTNQ MSVCKMFIIDKVDGDFCSLN EFSITGSTYAPEGEVLKNDK PIRSGQFDGLVELATICALC NDSSLDFNETKGVYEKVGEA TETALTTLVEKMNVFNTEVR NLSKVERANACNSVIRQLMK KEFTLEFSRDRKSMSVYCSP AKSSRAAVGNKMFVKGAPEG VIDRCNYVRVGTTRVPMTGP VKEKILSVIKEWGTGRDTLR CLALATRDTPPKREEMVLDD SSRFMEYETDLTFVGVVGML DPPRKEVMGSIQLCRDAGIR VIMITGDNKGTAIAICRRIG IFGENEEVADRAYTGREFDD LPLAEQREACRRACCFARVE PSHKSKIVEYLQSYDEITAM TGDGVNDAPALKKAEIGIAM GSGTAVAKTASEMVLADDNF STIVAAVEEGRAIYNNMKQF IRYLISSNVGEVVCIFLTAA LGLPEALIPVQLLWVNLVTD GLPATALGFNPPDLDIMDRP PRSPKEPLISGWLFFRYMAI GGYVGAATVGAAAWWFMYAE DGPGVTYHQLTHFMQCTEDH PHFEGLDCEIFEAPEPMTMA LSVLVTIEMCNALNSLSENQ SLMRMPPWVNIWLLGSICLS MSLHFLILYVDPLPMIFKLK ALDLTQWLMVLKISLPVIGL DEILKFIARNYLEDPEDERR K ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 1001 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 yes UNP P04191 . . . . . . . . . . . . . . . . 50721 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 50721 2 2 . GLU . 50721 2 3 . ALA . 50721 2 4 . ALA . 50721 2 5 . HIS . 50721 2 6 . SER . 50721 2 7 . LYS . 50721 2 8 . SER . 50721 2 9 . THR . 50721 2 10 . GLU . 50721 2 11 . GLU . 50721 2 12 . CYS . 50721 2 13 . LEU . 50721 2 14 . ALA . 50721 2 15 . TYR . 50721 2 16 . PHE . 50721 2 17 . GLY . 50721 2 18 . VAL . 50721 2 19 . SER . 50721 2 20 . GLU . 50721 2 21 . THR . 50721 2 22 . THR . 50721 2 23 . GLY . 50721 2 24 . LEU . 50721 2 25 . THR . 50721 2 26 . PRO . 50721 2 27 . ASP . 50721 2 28 . GLN . 50721 2 29 . VAL . 50721 2 30 . LYS . 50721 2 31 . ARG . 50721 2 32 . HIS . 50721 2 33 . LEU . 50721 2 34 . GLU . 50721 2 35 . LYS . 50721 2 36 . TYR . 50721 2 37 . GLY . 50721 2 38 . HIS . 50721 2 39 . ASN . 50721 2 40 . GLU . 50721 2 41 . LEU . 50721 2 42 . PRO . 50721 2 43 . ALA . 50721 2 44 . GLU . 50721 2 45 . GLU . 50721 2 46 . GLY . 50721 2 47 . LYS . 50721 2 48 . SER . 50721 2 49 . LEU . 50721 2 50 . TRP . 50721 2 51 . GLU . 50721 2 52 . LEU . 50721 2 53 . VAL . 50721 2 54 . ILE . 50721 2 55 . GLU . 50721 2 56 . GLN . 50721 2 57 . PHE . 50721 2 58 . GLU . 50721 2 59 . ASP . 50721 2 60 . LEU . 50721 2 61 . LEU . 50721 2 62 . VAL . 50721 2 63 . ARG . 50721 2 64 . ILE . 50721 2 65 . LEU . 50721 2 66 . LEU . 50721 2 67 . LEU . 50721 2 68 . ALA . 50721 2 69 . ALA . 50721 2 70 . CYS . 50721 2 71 . ILE . 50721 2 72 . SER . 50721 2 73 . PHE . 50721 2 74 . VAL . 50721 2 75 . LEU . 50721 2 76 . ALA . 50721 2 77 . TRP . 50721 2 78 . PHE . 50721 2 79 . GLU . 50721 2 80 . GLU . 50721 2 81 . GLY . 50721 2 82 . GLU . 50721 2 83 . GLU . 50721 2 84 . THR . 50721 2 85 . ILE . 50721 2 86 . THR . 50721 2 87 . ALA . 50721 2 88 . PHE . 50721 2 89 . VAL . 50721 2 90 . GLU . 50721 2 91 . PRO . 50721 2 92 . PHE . 50721 2 93 . VAL . 50721 2 94 . ILE . 50721 2 95 . LEU . 50721 2 96 . LEU . 50721 2 97 . ILE . 50721 2 98 . LEU . 50721 2 99 . ILE . 50721 2 100 . ALA . 50721 2 101 . ASN . 50721 2 102 . ALA . 50721 2 103 . ILE . 50721 2 104 . VAL . 50721 2 105 . GLY . 50721 2 106 . VAL . 50721 2 107 . TRP . 50721 2 108 . GLN . 50721 2 109 . GLU . 50721 2 110 . ARG . 50721 2 111 . ASN . 50721 2 112 . ALA . 50721 2 113 . GLU . 50721 2 114 . ASN . 50721 2 115 . ALA . 50721 2 116 . ILE . 50721 2 117 . GLU . 50721 2 118 . ALA . 50721 2 119 . LEU . 50721 2 120 . LYS . 50721 2 121 . GLU . 50721 2 122 . TYR . 50721 2 123 . GLU . 50721 2 124 . PRO . 50721 2 125 . GLU . 50721 2 126 . MET . 50721 2 127 . GLY . 50721 2 128 . LYS . 50721 2 129 . VAL . 50721 2 130 . TYR . 50721 2 131 . ARG . 50721 2 132 . ALA . 50721 2 133 . ASP . 50721 2 134 . ARG . 50721 2 135 . LYS . 50721 2 136 . SER . 50721 2 137 . VAL . 50721 2 138 . GLN . 50721 2 139 . ARG . 50721 2 140 . ILE . 50721 2 141 . LYS . 50721 2 142 . ALA . 50721 2 143 . ARG . 50721 2 144 . ASP . 50721 2 145 . ILE . 50721 2 146 . VAL . 50721 2 147 . PRO . 50721 2 148 . GLY . 50721 2 149 . ASP . 50721 2 150 . ILE . 50721 2 151 . VAL . 50721 2 152 . GLU . 50721 2 153 . VAL . 50721 2 154 . ALA . 50721 2 155 . VAL . 50721 2 156 . GLY . 50721 2 157 . ASP . 50721 2 158 . LYS . 50721 2 159 . VAL . 50721 2 160 . PRO . 50721 2 161 . ALA . 50721 2 162 . ASP . 50721 2 163 . ILE . 50721 2 164 . ARG . 50721 2 165 . ILE . 50721 2 166 . LEU . 50721 2 167 . SER . 50721 2 168 . ILE . 50721 2 169 . LYS . 50721 2 170 . SER . 50721 2 171 . THR . 50721 2 172 . THR . 50721 2 173 . LEU . 50721 2 174 . ARG . 50721 2 175 . VAL . 50721 2 176 . ASP . 50721 2 177 . GLN . 50721 2 178 . SER . 50721 2 179 . ILE . 50721 2 180 . LEU . 50721 2 181 . THR . 50721 2 182 . GLY . 50721 2 183 . GLU . 50721 2 184 . SER . 50721 2 185 . VAL . 50721 2 186 . SER . 50721 2 187 . VAL . 50721 2 188 . ILE . 50721 2 189 . LYS . 50721 2 190 . HIS . 50721 2 191 . THR . 50721 2 192 . GLU . 50721 2 193 . PRO . 50721 2 194 . VAL . 50721 2 195 . PRO . 50721 2 196 . ASP . 50721 2 197 . PRO . 50721 2 198 . ARG . 50721 2 199 . ALA . 50721 2 200 . VAL . 50721 2 201 . ASN . 50721 2 202 . GLN . 50721 2 203 . ASP . 50721 2 204 . LYS . 50721 2 205 . LYS . 50721 2 206 . ASN . 50721 2 207 . MET . 50721 2 208 . LEU . 50721 2 209 . PHE . 50721 2 210 . SER . 50721 2 211 . GLY . 50721 2 212 . THR . 50721 2 213 . ASN . 50721 2 214 . ILE . 50721 2 215 . ALA . 50721 2 216 . ALA . 50721 2 217 . GLY . 50721 2 218 . LYS . 50721 2 219 . ALA . 50721 2 220 . LEU . 50721 2 221 . GLY . 50721 2 222 . ILE . 50721 2 223 . VAL . 50721 2 224 . ALA . 50721 2 225 . THR . 50721 2 226 . THR . 50721 2 227 . GLY . 50721 2 228 . VAL . 50721 2 229 . SER . 50721 2 230 . THR . 50721 2 231 . GLU . 50721 2 232 . ILE . 50721 2 233 . GLY . 50721 2 234 . LYS . 50721 2 235 . ILE . 50721 2 236 . ARG . 50721 2 237 . ASP . 50721 2 238 . GLN . 50721 2 239 . MET . 50721 2 240 . ALA . 50721 2 241 . ALA . 50721 2 242 . THR . 50721 2 243 . GLU . 50721 2 244 . GLN . 50721 2 245 . ASP . 50721 2 246 . LYS . 50721 2 247 . THR . 50721 2 248 . PRO . 50721 2 249 . LEU . 50721 2 250 . GLN . 50721 2 251 . GLN . 50721 2 252 . LYS . 50721 2 253 . LEU . 50721 2 254 . ASP . 50721 2 255 . GLU . 50721 2 256 . PHE . 50721 2 257 . GLY . 50721 2 258 . GLU . 50721 2 259 . GLN . 50721 2 260 . LEU . 50721 2 261 . SER . 50721 2 262 . LYS . 50721 2 263 . VAL . 50721 2 264 . ILE . 50721 2 265 . SER . 50721 2 266 . LEU . 50721 2 267 . ILE . 50721 2 268 . CYS . 50721 2 269 . VAL . 50721 2 270 . ALA . 50721 2 271 . VAL . 50721 2 272 . TRP . 50721 2 273 . LEU . 50721 2 274 . ILE . 50721 2 275 . ASN . 50721 2 276 . ILE . 50721 2 277 . GLY . 50721 2 278 . HIS . 50721 2 279 . PHE . 50721 2 280 . ASN . 50721 2 281 . ASP . 50721 2 282 . PRO . 50721 2 283 . VAL . 50721 2 284 . HIS . 50721 2 285 . GLY . 50721 2 286 . GLY . 50721 2 287 . SER . 50721 2 288 . TRP . 50721 2 289 . ILE . 50721 2 290 . ARG . 50721 2 291 . GLY . 50721 2 292 . ALA . 50721 2 293 . ILE . 50721 2 294 . TYR . 50721 2 295 . TYR . 50721 2 296 . PHE . 50721 2 297 . LYS . 50721 2 298 . ILE . 50721 2 299 . ALA . 50721 2 300 . VAL . 50721 2 301 . ALA . 50721 2 302 . LEU . 50721 2 303 . ALA . 50721 2 304 . VAL . 50721 2 305 . ALA . 50721 2 306 . ALA . 50721 2 307 . ILE . 50721 2 308 . PRO . 50721 2 309 . GLU . 50721 2 310 . GLY . 50721 2 311 . LEU . 50721 2 312 . PRO . 50721 2 313 . ALA . 50721 2 314 . VAL . 50721 2 315 . ILE . 50721 2 316 . THR . 50721 2 317 . THR . 50721 2 318 . CYS . 50721 2 319 . LEU . 50721 2 320 . ALA . 50721 2 321 . LEU . 50721 2 322 . GLY . 50721 2 323 . THR . 50721 2 324 . ARG . 50721 2 325 . ARG . 50721 2 326 . MET . 50721 2 327 . ALA . 50721 2 328 . LYS . 50721 2 329 . LYS . 50721 2 330 . ASN . 50721 2 331 . ALA . 50721 2 332 . ILE . 50721 2 333 . VAL . 50721 2 334 . ARG . 50721 2 335 . SER . 50721 2 336 . LEU . 50721 2 337 . PRO . 50721 2 338 . SER . 50721 2 339 . VAL . 50721 2 340 . GLU . 50721 2 341 . THR . 50721 2 342 . LEU . 50721 2 343 . GLY . 50721 2 344 . CYS . 50721 2 345 . THR . 50721 2 346 . SER . 50721 2 347 . VAL . 50721 2 348 . ILE . 50721 2 349 . CYS . 50721 2 350 . SER . 50721 2 351 . ASP . 50721 2 352 . LYS . 50721 2 353 . THR . 50721 2 354 . GLY . 50721 2 355 . THR . 50721 2 356 . LEU . 50721 2 357 . THR . 50721 2 358 . THR . 50721 2 359 . ASN . 50721 2 360 . GLN . 50721 2 361 . MET . 50721 2 362 . SER . 50721 2 363 . VAL . 50721 2 364 . CYS . 50721 2 365 . LYS . 50721 2 366 . MET . 50721 2 367 . PHE . 50721 2 368 . ILE . 50721 2 369 . ILE . 50721 2 370 . ASP . 50721 2 371 . LYS . 50721 2 372 . VAL . 50721 2 373 . ASP . 50721 2 374 . GLY . 50721 2 375 . ASP . 50721 2 376 . PHE . 50721 2 377 . CYS . 50721 2 378 . SER . 50721 2 379 . LEU . 50721 2 380 . ASN . 50721 2 381 . GLU . 50721 2 382 . PHE . 50721 2 383 . SER . 50721 2 384 . ILE . 50721 2 385 . THR . 50721 2 386 . GLY . 50721 2 387 . SER . 50721 2 388 . THR . 50721 2 389 . TYR . 50721 2 390 . ALA . 50721 2 391 . PRO . 50721 2 392 . GLU . 50721 2 393 . GLY . 50721 2 394 . GLU . 50721 2 395 . VAL . 50721 2 396 . LEU . 50721 2 397 . LYS . 50721 2 398 . ASN . 50721 2 399 . ASP . 50721 2 400 . LYS . 50721 2 401 . PRO . 50721 2 402 . ILE . 50721 2 403 . ARG . 50721 2 404 . SER . 50721 2 405 . GLY . 50721 2 406 . GLN . 50721 2 407 . PHE . 50721 2 408 . ASP . 50721 2 409 . GLY . 50721 2 410 . LEU . 50721 2 411 . VAL . 50721 2 412 . GLU . 50721 2 413 . LEU . 50721 2 414 . ALA . 50721 2 415 . THR . 50721 2 416 . ILE . 50721 2 417 . CYS . 50721 2 418 . ALA . 50721 2 419 . LEU . 50721 2 420 . CYS . 50721 2 421 . ASN . 50721 2 422 . ASP . 50721 2 423 . SER . 50721 2 424 . SER . 50721 2 425 . LEU . 50721 2 426 . ASP . 50721 2 427 . PHE . 50721 2 428 . ASN . 50721 2 429 . GLU . 50721 2 430 . THR . 50721 2 431 . LYS . 50721 2 432 . GLY . 50721 2 433 . VAL . 50721 2 434 . TYR . 50721 2 435 . GLU . 50721 2 436 . LYS . 50721 2 437 . VAL . 50721 2 438 . GLY . 50721 2 439 . GLU . 50721 2 440 . ALA . 50721 2 441 . THR . 50721 2 442 . GLU . 50721 2 443 . THR . 50721 2 444 . ALA . 50721 2 445 . LEU . 50721 2 446 . THR . 50721 2 447 . THR . 50721 2 448 . LEU . 50721 2 449 . VAL . 50721 2 450 . GLU . 50721 2 451 . LYS . 50721 2 452 . MET . 50721 2 453 . ASN . 50721 2 454 . VAL . 50721 2 455 . PHE . 50721 2 456 . ASN . 50721 2 457 . THR . 50721 2 458 . GLU . 50721 2 459 . VAL . 50721 2 460 . ARG . 50721 2 461 . ASN . 50721 2 462 . LEU . 50721 2 463 . SER . 50721 2 464 . LYS . 50721 2 465 . VAL . 50721 2 466 . GLU . 50721 2 467 . ARG . 50721 2 468 . ALA . 50721 2 469 . ASN . 50721 2 470 . ALA . 50721 2 471 . CYS . 50721 2 472 . ASN . 50721 2 473 . SER . 50721 2 474 . VAL . 50721 2 475 . ILE . 50721 2 476 . ARG . 50721 2 477 . GLN . 50721 2 478 . LEU . 50721 2 479 . MET . 50721 2 480 . LYS . 50721 2 481 . LYS . 50721 2 482 . GLU . 50721 2 483 . PHE . 50721 2 484 . THR . 50721 2 485 . LEU . 50721 2 486 . GLU . 50721 2 487 . PHE . 50721 2 488 . SER . 50721 2 489 . ARG . 50721 2 490 . ASP . 50721 2 491 . ARG . 50721 2 492 . LYS . 50721 2 493 . SER . 50721 2 494 . MET . 50721 2 495 . SER . 50721 2 496 . VAL . 50721 2 497 . TYR . 50721 2 498 . CYS . 50721 2 499 . SER . 50721 2 500 . PRO . 50721 2 501 . ALA . 50721 2 502 . LYS . 50721 2 503 . SER . 50721 2 504 . SER . 50721 2 505 . ARG . 50721 2 506 . ALA . 50721 2 507 . ALA . 50721 2 508 . VAL . 50721 2 509 . GLY . 50721 2 510 . ASN . 50721 2 511 . LYS . 50721 2 512 . MET . 50721 2 513 . PHE . 50721 2 514 . VAL . 50721 2 515 . LYS . 50721 2 516 . GLY . 50721 2 517 . ALA . 50721 2 518 . PRO . 50721 2 519 . GLU . 50721 2 520 . GLY . 50721 2 521 . VAL . 50721 2 522 . ILE . 50721 2 523 . ASP . 50721 2 524 . ARG . 50721 2 525 . CYS . 50721 2 526 . ASN . 50721 2 527 . TYR . 50721 2 528 . VAL . 50721 2 529 . ARG . 50721 2 530 . VAL . 50721 2 531 . GLY . 50721 2 532 . THR . 50721 2 533 . THR . 50721 2 534 . ARG . 50721 2 535 . VAL . 50721 2 536 . PRO . 50721 2 537 . MET . 50721 2 538 . THR . 50721 2 539 . GLY . 50721 2 540 . PRO . 50721 2 541 . VAL . 50721 2 542 . LYS . 50721 2 543 . GLU . 50721 2 544 . LYS . 50721 2 545 . ILE . 50721 2 546 . LEU . 50721 2 547 . SER . 50721 2 548 . VAL . 50721 2 549 . ILE . 50721 2 550 . LYS . 50721 2 551 . GLU . 50721 2 552 . TRP . 50721 2 553 . GLY . 50721 2 554 . THR . 50721 2 555 . GLY . 50721 2 556 . ARG . 50721 2 557 . ASP . 50721 2 558 . THR . 50721 2 559 . LEU . 50721 2 560 . ARG . 50721 2 561 . CYS . 50721 2 562 . LEU . 50721 2 563 . ALA . 50721 2 564 . LEU . 50721 2 565 . ALA . 50721 2 566 . THR . 50721 2 567 . ARG . 50721 2 568 . ASP . 50721 2 569 . THR . 50721 2 570 . PRO . 50721 2 571 . PRO . 50721 2 572 . LYS . 50721 2 573 . ARG . 50721 2 574 . GLU . 50721 2 575 . GLU . 50721 2 576 . MET . 50721 2 577 . VAL . 50721 2 578 . LEU . 50721 2 579 . ASP . 50721 2 580 . ASP . 50721 2 581 . SER . 50721 2 582 . SER . 50721 2 583 . ARG . 50721 2 584 . PHE . 50721 2 585 . MET . 50721 2 586 . GLU . 50721 2 587 . TYR . 50721 2 588 . GLU . 50721 2 589 . THR . 50721 2 590 . ASP . 50721 2 591 . LEU . 50721 2 592 . THR . 50721 2 593 . PHE . 50721 2 594 . VAL . 50721 2 595 . GLY . 50721 2 596 . VAL . 50721 2 597 . VAL . 50721 2 598 . GLY . 50721 2 599 . MET . 50721 2 600 . LEU . 50721 2 601 . ASP . 50721 2 602 . PRO . 50721 2 603 . PRO . 50721 2 604 . ARG . 50721 2 605 . LYS . 50721 2 606 . GLU . 50721 2 607 . VAL . 50721 2 608 . MET . 50721 2 609 . GLY . 50721 2 610 . SER . 50721 2 611 . ILE . 50721 2 612 . GLN . 50721 2 613 . LEU . 50721 2 614 . CYS . 50721 2 615 . ARG . 50721 2 616 . ASP . 50721 2 617 . ALA . 50721 2 618 . GLY . 50721 2 619 . ILE . 50721 2 620 . ARG . 50721 2 621 . VAL . 50721 2 622 . ILE . 50721 2 623 . MET . 50721 2 624 . ILE . 50721 2 625 . THR . 50721 2 626 . GLY . 50721 2 627 . ASP . 50721 2 628 . ASN . 50721 2 629 . LYS . 50721 2 630 . GLY . 50721 2 631 . THR . 50721 2 632 . ALA . 50721 2 633 . ILE . 50721 2 634 . ALA . 50721 2 635 . ILE . 50721 2 636 . CYS . 50721 2 637 . ARG . 50721 2 638 . ARG . 50721 2 639 . ILE . 50721 2 640 . GLY . 50721 2 641 . ILE . 50721 2 642 . PHE . 50721 2 643 . GLY . 50721 2 644 . GLU . 50721 2 645 . ASN . 50721 2 646 . GLU . 50721 2 647 . GLU . 50721 2 648 . VAL . 50721 2 649 . ALA . 50721 2 650 . ASP . 50721 2 651 . ARG . 50721 2 652 . ALA . 50721 2 653 . TYR . 50721 2 654 . THR . 50721 2 655 . GLY . 50721 2 656 . ARG . 50721 2 657 . GLU . 50721 2 658 . PHE . 50721 2 659 . ASP . 50721 2 660 . ASP . 50721 2 661 . LEU . 50721 2 662 . PRO . 50721 2 663 . LEU . 50721 2 664 . ALA . 50721 2 665 . GLU . 50721 2 666 . GLN . 50721 2 667 . ARG . 50721 2 668 . GLU . 50721 2 669 . ALA . 50721 2 670 . CYS . 50721 2 671 . ARG . 50721 2 672 . ARG . 50721 2 673 . ALA . 50721 2 674 . CYS . 50721 2 675 . CYS . 50721 2 676 . PHE . 50721 2 677 . ALA . 50721 2 678 . ARG . 50721 2 679 . VAL . 50721 2 680 . GLU . 50721 2 681 . PRO . 50721 2 682 . SER . 50721 2 683 . HIS . 50721 2 684 . LYS . 50721 2 685 . SER . 50721 2 686 . LYS . 50721 2 687 . ILE . 50721 2 688 . VAL . 50721 2 689 . GLU . 50721 2 690 . TYR . 50721 2 691 . LEU . 50721 2 692 . GLN . 50721 2 693 . SER . 50721 2 694 . TYR . 50721 2 695 . ASP . 50721 2 696 . GLU . 50721 2 697 . ILE . 50721 2 698 . THR . 50721 2 699 . ALA . 50721 2 700 . MET . 50721 2 701 . THR . 50721 2 702 . GLY . 50721 2 703 . ASP . 50721 2 704 . GLY . 50721 2 705 . VAL . 50721 2 706 . ASN . 50721 2 707 . ASP . 50721 2 708 . ALA . 50721 2 709 . PRO . 50721 2 710 . ALA . 50721 2 711 . LEU . 50721 2 712 . LYS . 50721 2 713 . LYS . 50721 2 714 . ALA . 50721 2 715 . GLU . 50721 2 716 . ILE . 50721 2 717 . GLY . 50721 2 718 . ILE . 50721 2 719 . ALA . 50721 2 720 . MET . 50721 2 721 . GLY . 50721 2 722 . SER . 50721 2 723 . GLY . 50721 2 724 . THR . 50721 2 725 . ALA . 50721 2 726 . VAL . 50721 2 727 . ALA . 50721 2 728 . LYS . 50721 2 729 . THR . 50721 2 730 . ALA . 50721 2 731 . SER . 50721 2 732 . GLU . 50721 2 733 . MET . 50721 2 734 . VAL . 50721 2 735 . LEU . 50721 2 736 . ALA . 50721 2 737 . ASP . 50721 2 738 . ASP . 50721 2 739 . ASN . 50721 2 740 . PHE . 50721 2 741 . SER . 50721 2 742 . THR . 50721 2 743 . ILE . 50721 2 744 . VAL . 50721 2 745 . ALA . 50721 2 746 . ALA . 50721 2 747 . VAL . 50721 2 748 . GLU . 50721 2 749 . GLU . 50721 2 750 . GLY . 50721 2 751 . ARG . 50721 2 752 . ALA . 50721 2 753 . ILE . 50721 2 754 . TYR . 50721 2 755 . ASN . 50721 2 756 . ASN . 50721 2 757 . MET . 50721 2 758 . LYS . 50721 2 759 . GLN . 50721 2 760 . PHE . 50721 2 761 . ILE . 50721 2 762 . ARG . 50721 2 763 . TYR . 50721 2 764 . LEU . 50721 2 765 . ILE . 50721 2 766 . SER . 50721 2 767 . SER . 50721 2 768 . ASN . 50721 2 769 . VAL . 50721 2 770 . GLY . 50721 2 771 . GLU . 50721 2 772 . VAL . 50721 2 773 . VAL . 50721 2 774 . CYS . 50721 2 775 . ILE . 50721 2 776 . PHE . 50721 2 777 . LEU . 50721 2 778 . THR . 50721 2 779 . ALA . 50721 2 780 . ALA . 50721 2 781 . LEU . 50721 2 782 . GLY . 50721 2 783 . LEU . 50721 2 784 . PRO . 50721 2 785 . GLU . 50721 2 786 . ALA . 50721 2 787 . LEU . 50721 2 788 . ILE . 50721 2 789 . PRO . 50721 2 790 . VAL . 50721 2 791 . GLN . 50721 2 792 . LEU . 50721 2 793 . LEU . 50721 2 794 . TRP . 50721 2 795 . VAL . 50721 2 796 . ASN . 50721 2 797 . LEU . 50721 2 798 . VAL . 50721 2 799 . THR . 50721 2 800 . ASP . 50721 2 801 . GLY . 50721 2 802 . LEU . 50721 2 803 . PRO . 50721 2 804 . ALA . 50721 2 805 . THR . 50721 2 806 . ALA . 50721 2 807 . LEU . 50721 2 808 . GLY . 50721 2 809 . PHE . 50721 2 810 . ASN . 50721 2 811 . PRO . 50721 2 812 . PRO . 50721 2 813 . ASP . 50721 2 814 . LEU . 50721 2 815 . ASP . 50721 2 816 . ILE . 50721 2 817 . MET . 50721 2 818 . ASP . 50721 2 819 . ARG . 50721 2 820 . PRO . 50721 2 821 . PRO . 50721 2 822 . ARG . 50721 2 823 . SER . 50721 2 824 . PRO . 50721 2 825 . LYS . 50721 2 826 . GLU . 50721 2 827 . PRO . 50721 2 828 . LEU . 50721 2 829 . ILE . 50721 2 830 . SER . 50721 2 831 . GLY . 50721 2 832 . TRP . 50721 2 833 . LEU . 50721 2 834 . PHE . 50721 2 835 . PHE . 50721 2 836 . ARG . 50721 2 837 . TYR . 50721 2 838 . MET . 50721 2 839 . ALA . 50721 2 840 . ILE . 50721 2 841 . GLY . 50721 2 842 . GLY . 50721 2 843 . TYR . 50721 2 844 . VAL . 50721 2 845 . GLY . 50721 2 846 . ALA . 50721 2 847 . ALA . 50721 2 848 . THR . 50721 2 849 . VAL . 50721 2 850 . GLY . 50721 2 851 . ALA . 50721 2 852 . ALA . 50721 2 853 . ALA . 50721 2 854 . TRP . 50721 2 855 . TRP . 50721 2 856 . PHE . 50721 2 857 . MET . 50721 2 858 . TYR . 50721 2 859 . ALA . 50721 2 860 . GLU . 50721 2 861 . ASP . 50721 2 862 . GLY . 50721 2 863 . PRO . 50721 2 864 . GLY . 50721 2 865 . VAL . 50721 2 866 . THR . 50721 2 867 . TYR . 50721 2 868 . HIS . 50721 2 869 . GLN . 50721 2 870 . LEU . 50721 2 871 . THR . 50721 2 872 . HIS . 50721 2 873 . PHE . 50721 2 874 . MET . 50721 2 875 . GLN . 50721 2 876 . CYS . 50721 2 877 . THR . 50721 2 878 . GLU . 50721 2 879 . ASP . 50721 2 880 . HIS . 50721 2 881 . PRO . 50721 2 882 . HIS . 50721 2 883 . PHE . 50721 2 884 . GLU . 50721 2 885 . GLY . 50721 2 886 . LEU . 50721 2 887 . ASP . 50721 2 888 . CYS . 50721 2 889 . GLU . 50721 2 890 . ILE . 50721 2 891 . PHE . 50721 2 892 . GLU . 50721 2 893 . ALA . 50721 2 894 . PRO . 50721 2 895 . GLU . 50721 2 896 . PRO . 50721 2 897 . MET . 50721 2 898 . THR . 50721 2 899 . MET . 50721 2 900 . ALA . 50721 2 901 . LEU . 50721 2 902 . SER . 50721 2 903 . VAL . 50721 2 904 . LEU . 50721 2 905 . VAL . 50721 2 906 . THR . 50721 2 907 . ILE . 50721 2 908 . GLU . 50721 2 909 . MET . 50721 2 910 . CYS . 50721 2 911 . ASN . 50721 2 912 . ALA . 50721 2 913 . LEU . 50721 2 914 . ASN . 50721 2 915 . SER . 50721 2 916 . LEU . 50721 2 917 . SER . 50721 2 918 . GLU . 50721 2 919 . ASN . 50721 2 920 . GLN . 50721 2 921 . SER . 50721 2 922 . LEU . 50721 2 923 . MET . 50721 2 924 . ARG . 50721 2 925 . MET . 50721 2 926 . PRO . 50721 2 927 . PRO . 50721 2 928 . TRP . 50721 2 929 . VAL . 50721 2 930 . ASN . 50721 2 931 . ILE . 50721 2 932 . TRP . 50721 2 933 . LEU . 50721 2 934 . LEU . 50721 2 935 . GLY . 50721 2 936 . SER . 50721 2 937 . ILE . 50721 2 938 . CYS . 50721 2 939 . LEU . 50721 2 940 . SER . 50721 2 941 . MET . 50721 2 942 . SER . 50721 2 943 . LEU . 50721 2 944 . HIS . 50721 2 945 . PHE . 50721 2 946 . LEU . 50721 2 947 . ILE . 50721 2 948 . LEU . 50721 2 949 . TYR . 50721 2 950 . VAL . 50721 2 951 . ASP . 50721 2 952 . PRO . 50721 2 953 . LEU . 50721 2 954 . PRO . 50721 2 955 . MET . 50721 2 956 . ILE . 50721 2 957 . PHE . 50721 2 958 . LYS . 50721 2 959 . LEU . 50721 2 960 . LYS . 50721 2 961 . ALA . 50721 2 962 . LEU . 50721 2 963 . ASP . 50721 2 964 . LEU . 50721 2 965 . THR . 50721 2 966 . GLN . 50721 2 967 . TRP . 50721 2 968 . LEU . 50721 2 969 . MET . 50721 2 970 . VAL . 50721 2 971 . LEU . 50721 2 972 . LYS . 50721 2 973 . ILE . 50721 2 974 . SER . 50721 2 975 . LEU . 50721 2 976 . PRO . 50721 2 977 . VAL . 50721 2 978 . ILE . 50721 2 979 . GLY . 50721 2 980 . LEU . 50721 2 981 . ASP . 50721 2 982 . GLU . 50721 2 983 . ILE . 50721 2 984 . LEU . 50721 2 985 . LYS . 50721 2 986 . PHE . 50721 2 987 . ILE . 50721 2 988 . ALA . 50721 2 989 . ARG . 50721 2 990 . ASN . 50721 2 991 . TYR . 50721 2 992 . LEU . 50721 2 993 . GLU . 50721 2 994 . ASP . 50721 2 995 . PRO . 50721 2 996 . GLU . 50721 2 997 . ASP . 50721 2 998 . GLU . 50721 2 999 . ARG . 50721 2 1000 . ARG . 50721 2 1001 . LYS . 50721 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 50721 2 . GLU 2 2 50721 2 . ALA 3 3 50721 2 . ALA 4 4 50721 2 . HIS 5 5 50721 2 . SER 6 6 50721 2 . LYS 7 7 50721 2 . SER 8 8 50721 2 . THR 9 9 50721 2 . GLU 10 10 50721 2 . GLU 11 11 50721 2 . CYS 12 12 50721 2 . LEU 13 13 50721 2 . ALA 14 14 50721 2 . TYR 15 15 50721 2 . PHE 16 16 50721 2 . GLY 17 17 50721 2 . VAL 18 18 50721 2 . SER 19 19 50721 2 . GLU 20 20 50721 2 . THR 21 21 50721 2 . THR 22 22 50721 2 . GLY 23 23 50721 2 . LEU 24 24 50721 2 . THR 25 25 50721 2 . PRO 26 26 50721 2 . ASP 27 27 50721 2 . GLN 28 28 50721 2 . VAL 29 29 50721 2 . LYS 30 30 50721 2 . ARG 31 31 50721 2 . HIS 32 32 50721 2 . LEU 33 33 50721 2 . GLU 34 34 50721 2 . LYS 35 35 50721 2 . TYR 36 36 50721 2 . GLY 37 37 50721 2 . HIS 38 38 50721 2 . ASN 39 39 50721 2 . GLU 40 40 50721 2 . LEU 41 41 50721 2 . PRO 42 42 50721 2 . ALA 43 43 50721 2 . GLU 44 44 50721 2 . GLU 45 45 50721 2 . GLY 46 46 50721 2 . LYS 47 47 50721 2 . SER 48 48 50721 2 . LEU 49 49 50721 2 . TRP 50 50 50721 2 . GLU 51 51 50721 2 . LEU 52 52 50721 2 . VAL 53 53 50721 2 . ILE 54 54 50721 2 . GLU 55 55 50721 2 . GLN 56 56 50721 2 . PHE 57 57 50721 2 . GLU 58 58 50721 2 . ASP 59 59 50721 2 . LEU 60 60 50721 2 . LEU 61 61 50721 2 . VAL 62 62 50721 2 . ARG 63 63 50721 2 . ILE 64 64 50721 2 . LEU 65 65 50721 2 . LEU 66 66 50721 2 . LEU 67 67 50721 2 . ALA 68 68 50721 2 . ALA 69 69 50721 2 . CYS 70 70 50721 2 . ILE 71 71 50721 2 . SER 72 72 50721 2 . PHE 73 73 50721 2 . VAL 74 74 50721 2 . LEU 75 75 50721 2 . ALA 76 76 50721 2 . TRP 77 77 50721 2 . PHE 78 78 50721 2 . GLU 79 79 50721 2 . GLU 80 80 50721 2 . GLY 81 81 50721 2 . GLU 82 82 50721 2 . GLU 83 83 50721 2 . THR 84 84 50721 2 . ILE 85 85 50721 2 . THR 86 86 50721 2 . ALA 87 87 50721 2 . PHE 88 88 50721 2 . VAL 89 89 50721 2 . GLU 90 90 50721 2 . PRO 91 91 50721 2 . PHE 92 92 50721 2 . VAL 93 93 50721 2 . ILE 94 94 50721 2 . LEU 95 95 50721 2 . LEU 96 96 50721 2 . ILE 97 97 50721 2 . LEU 98 98 50721 2 . ILE 99 99 50721 2 . ALA 100 100 50721 2 . ASN 101 101 50721 2 . ALA 102 102 50721 2 . ILE 103 103 50721 2 . VAL 104 104 50721 2 . GLY 105 105 50721 2 . VAL 106 106 50721 2 . TRP 107 107 50721 2 . GLN 108 108 50721 2 . GLU 109 109 50721 2 . ARG 110 110 50721 2 . ASN 111 111 50721 2 . ALA 112 112 50721 2 . GLU 113 113 50721 2 . ASN 114 114 50721 2 . ALA 115 115 50721 2 . ILE 116 116 50721 2 . GLU 117 117 50721 2 . ALA 118 118 50721 2 . LEU 119 119 50721 2 . LYS 120 120 50721 2 . GLU 121 121 50721 2 . TYR 122 122 50721 2 . GLU 123 123 50721 2 . PRO 124 124 50721 2 . GLU 125 125 50721 2 . MET 126 126 50721 2 . GLY 127 127 50721 2 . LYS 128 128 50721 2 . VAL 129 129 50721 2 . TYR 130 130 50721 2 . ARG 131 131 50721 2 . ALA 132 132 50721 2 . ASP 133 133 50721 2 . ARG 134 134 50721 2 . LYS 135 135 50721 2 . SER 136 136 50721 2 . VAL 137 137 50721 2 . GLN 138 138 50721 2 . ARG 139 139 50721 2 . ILE 140 140 50721 2 . LYS 141 141 50721 2 . ALA 142 142 50721 2 . ARG 143 143 50721 2 . ASP 144 144 50721 2 . ILE 145 145 50721 2 . VAL 146 146 50721 2 . PRO 147 147 50721 2 . GLY 148 148 50721 2 . ASP 149 149 50721 2 . ILE 150 150 50721 2 . VAL 151 151 50721 2 . GLU 152 152 50721 2 . VAL 153 153 50721 2 . ALA 154 154 50721 2 . VAL 155 155 50721 2 . GLY 156 156 50721 2 . ASP 157 157 50721 2 . LYS 158 158 50721 2 . VAL 159 159 50721 2 . PRO 160 160 50721 2 . ALA 161 161 50721 2 . ASP 162 162 50721 2 . ILE 163 163 50721 2 . ARG 164 164 50721 2 . ILE 165 165 50721 2 . LEU 166 166 50721 2 . SER 167 167 50721 2 . ILE 168 168 50721 2 . LYS 169 169 50721 2 . SER 170 170 50721 2 . THR 171 171 50721 2 . THR 172 172 50721 2 . LEU 173 173 50721 2 . ARG 174 174 50721 2 . VAL 175 175 50721 2 . ASP 176 176 50721 2 . GLN 177 177 50721 2 . SER 178 178 50721 2 . ILE 179 179 50721 2 . LEU 180 180 50721 2 . THR 181 181 50721 2 . GLY 182 182 50721 2 . GLU 183 183 50721 2 . SER 184 184 50721 2 . VAL 185 185 50721 2 . SER 186 186 50721 2 . VAL 187 187 50721 2 . ILE 188 188 50721 2 . LYS 189 189 50721 2 . HIS 190 190 50721 2 . THR 191 191 50721 2 . GLU 192 192 50721 2 . PRO 193 193 50721 2 . VAL 194 194 50721 2 . PRO 195 195 50721 2 . ASP 196 196 50721 2 . PRO 197 197 50721 2 . ARG 198 198 50721 2 . ALA 199 199 50721 2 . VAL 200 200 50721 2 . ASN 201 201 50721 2 . GLN 202 202 50721 2 . ASP 203 203 50721 2 . LYS 204 204 50721 2 . LYS 205 205 50721 2 . ASN 206 206 50721 2 . MET 207 207 50721 2 . LEU 208 208 50721 2 . PHE 209 209 50721 2 . SER 210 210 50721 2 . GLY 211 211 50721 2 . THR 212 212 50721 2 . ASN 213 213 50721 2 . ILE 214 214 50721 2 . ALA 215 215 50721 2 . ALA 216 216 50721 2 . GLY 217 217 50721 2 . LYS 218 218 50721 2 . ALA 219 219 50721 2 . LEU 220 220 50721 2 . GLY 221 221 50721 2 . ILE 222 222 50721 2 . VAL 223 223 50721 2 . ALA 224 224 50721 2 . THR 225 225 50721 2 . THR 226 226 50721 2 . GLY 227 227 50721 2 . VAL 228 228 50721 2 . SER 229 229 50721 2 . THR 230 230 50721 2 . GLU 231 231 50721 2 . ILE 232 232 50721 2 . GLY 233 233 50721 2 . LYS 234 234 50721 2 . ILE 235 235 50721 2 . ARG 236 236 50721 2 . ASP 237 237 50721 2 . GLN 238 238 50721 2 . MET 239 239 50721 2 . ALA 240 240 50721 2 . ALA 241 241 50721 2 . THR 242 242 50721 2 . GLU 243 243 50721 2 . GLN 244 244 50721 2 . ASP 245 245 50721 2 . LYS 246 246 50721 2 . THR 247 247 50721 2 . PRO 248 248 50721 2 . LEU 249 249 50721 2 . GLN 250 250 50721 2 . GLN 251 251 50721 2 . LYS 252 252 50721 2 . LEU 253 253 50721 2 . ASP 254 254 50721 2 . GLU 255 255 50721 2 . PHE 256 256 50721 2 . GLY 257 257 50721 2 . GLU 258 258 50721 2 . GLN 259 259 50721 2 . LEU 260 260 50721 2 . SER 261 261 50721 2 . LYS 262 262 50721 2 . VAL 263 263 50721 2 . ILE 264 264 50721 2 . SER 265 265 50721 2 . LEU 266 266 50721 2 . ILE 267 267 50721 2 . CYS 268 268 50721 2 . VAL 269 269 50721 2 . ALA 270 270 50721 2 . VAL 271 271 50721 2 . TRP 272 272 50721 2 . LEU 273 273 50721 2 . ILE 274 274 50721 2 . ASN 275 275 50721 2 . ILE 276 276 50721 2 . GLY 277 277 50721 2 . HIS 278 278 50721 2 . PHE 279 279 50721 2 . ASN 280 280 50721 2 . ASP 281 281 50721 2 . PRO 282 282 50721 2 . VAL 283 283 50721 2 . HIS 284 284 50721 2 . GLY 285 285 50721 2 . GLY 286 286 50721 2 . SER 287 287 50721 2 . TRP 288 288 50721 2 . ILE 289 289 50721 2 . ARG 290 290 50721 2 . GLY 291 291 50721 2 . ALA 292 292 50721 2 . ILE 293 293 50721 2 . TYR 294 294 50721 2 . TYR 295 295 50721 2 . PHE 296 296 50721 2 . LYS 297 297 50721 2 . ILE 298 298 50721 2 . ALA 299 299 50721 2 . VAL 300 300 50721 2 . ALA 301 301 50721 2 . LEU 302 302 50721 2 . ALA 303 303 50721 2 . VAL 304 304 50721 2 . ALA 305 305 50721 2 . ALA 306 306 50721 2 . ILE 307 307 50721 2 . PRO 308 308 50721 2 . GLU 309 309 50721 2 . GLY 310 310 50721 2 . LEU 311 311 50721 2 . PRO 312 312 50721 2 . ALA 313 313 50721 2 . VAL 314 314 50721 2 . ILE 315 315 50721 2 . THR 316 316 50721 2 . THR 317 317 50721 2 . CYS 318 318 50721 2 . LEU 319 319 50721 2 . ALA 320 320 50721 2 . LEU 321 321 50721 2 . GLY 322 322 50721 2 . THR 323 323 50721 2 . ARG 324 324 50721 2 . ARG 325 325 50721 2 . MET 326 326 50721 2 . ALA 327 327 50721 2 . LYS 328 328 50721 2 . LYS 329 329 50721 2 . ASN 330 330 50721 2 . ALA 331 331 50721 2 . ILE 332 332 50721 2 . VAL 333 333 50721 2 . ARG 334 334 50721 2 . SER 335 335 50721 2 . LEU 336 336 50721 2 . PRO 337 337 50721 2 . SER 338 338 50721 2 . VAL 339 339 50721 2 . GLU 340 340 50721 2 . THR 341 341 50721 2 . LEU 342 342 50721 2 . GLY 343 343 50721 2 . CYS 344 344 50721 2 . THR 345 345 50721 2 . SER 346 346 50721 2 . VAL 347 347 50721 2 . ILE 348 348 50721 2 . CYS 349 349 50721 2 . SER 350 350 50721 2 . ASP 351 351 50721 2 . LYS 352 352 50721 2 . THR 353 353 50721 2 . GLY 354 354 50721 2 . THR 355 355 50721 2 . LEU 356 356 50721 2 . THR 357 357 50721 2 . THR 358 358 50721 2 . ASN 359 359 50721 2 . GLN 360 360 50721 2 . MET 361 361 50721 2 . SER 362 362 50721 2 . VAL 363 363 50721 2 . CYS 364 364 50721 2 . LYS 365 365 50721 2 . MET 366 366 50721 2 . PHE 367 367 50721 2 . ILE 368 368 50721 2 . ILE 369 369 50721 2 . ASP 370 370 50721 2 . LYS 371 371 50721 2 . VAL 372 372 50721 2 . ASP 373 373 50721 2 . GLY 374 374 50721 2 . ASP 375 375 50721 2 . PHE 376 376 50721 2 . CYS 377 377 50721 2 . SER 378 378 50721 2 . LEU 379 379 50721 2 . ASN 380 380 50721 2 . GLU 381 381 50721 2 . PHE 382 382 50721 2 . SER 383 383 50721 2 . ILE 384 384 50721 2 . THR 385 385 50721 2 . GLY 386 386 50721 2 . SER 387 387 50721 2 . THR 388 388 50721 2 . TYR 389 389 50721 2 . ALA 390 390 50721 2 . PRO 391 391 50721 2 . GLU 392 392 50721 2 . GLY 393 393 50721 2 . GLU 394 394 50721 2 . VAL 395 395 50721 2 . LEU 396 396 50721 2 . LYS 397 397 50721 2 . ASN 398 398 50721 2 . ASP 399 399 50721 2 . LYS 400 400 50721 2 . PRO 401 401 50721 2 . ILE 402 402 50721 2 . ARG 403 403 50721 2 . SER 404 404 50721 2 . GLY 405 405 50721 2 . GLN 406 406 50721 2 . PHE 407 407 50721 2 . ASP 408 408 50721 2 . GLY 409 409 50721 2 . LEU 410 410 50721 2 . VAL 411 411 50721 2 . GLU 412 412 50721 2 . LEU 413 413 50721 2 . ALA 414 414 50721 2 . THR 415 415 50721 2 . ILE 416 416 50721 2 . CYS 417 417 50721 2 . ALA 418 418 50721 2 . LEU 419 419 50721 2 . CYS 420 420 50721 2 . ASN 421 421 50721 2 . ASP 422 422 50721 2 . SER 423 423 50721 2 . SER 424 424 50721 2 . LEU 425 425 50721 2 . ASP 426 426 50721 2 . PHE 427 427 50721 2 . ASN 428 428 50721 2 . GLU 429 429 50721 2 . THR 430 430 50721 2 . LYS 431 431 50721 2 . GLY 432 432 50721 2 . VAL 433 433 50721 2 . TYR 434 434 50721 2 . GLU 435 435 50721 2 . LYS 436 436 50721 2 . VAL 437 437 50721 2 . GLY 438 438 50721 2 . GLU 439 439 50721 2 . ALA 440 440 50721 2 . THR 441 441 50721 2 . GLU 442 442 50721 2 . THR 443 443 50721 2 . ALA 444 444 50721 2 . LEU 445 445 50721 2 . THR 446 446 50721 2 . THR 447 447 50721 2 . LEU 448 448 50721 2 . VAL 449 449 50721 2 . GLU 450 450 50721 2 . LYS 451 451 50721 2 . MET 452 452 50721 2 . ASN 453 453 50721 2 . VAL 454 454 50721 2 . PHE 455 455 50721 2 . ASN 456 456 50721 2 . THR 457 457 50721 2 . GLU 458 458 50721 2 . VAL 459 459 50721 2 . ARG 460 460 50721 2 . ASN 461 461 50721 2 . LEU 462 462 50721 2 . SER 463 463 50721 2 . LYS 464 464 50721 2 . VAL 465 465 50721 2 . GLU 466 466 50721 2 . ARG 467 467 50721 2 . ALA 468 468 50721 2 . ASN 469 469 50721 2 . ALA 470 470 50721 2 . CYS 471 471 50721 2 . ASN 472 472 50721 2 . SER 473 473 50721 2 . VAL 474 474 50721 2 . ILE 475 475 50721 2 . ARG 476 476 50721 2 . GLN 477 477 50721 2 . LEU 478 478 50721 2 . MET 479 479 50721 2 . LYS 480 480 50721 2 . LYS 481 481 50721 2 . GLU 482 482 50721 2 . PHE 483 483 50721 2 . THR 484 484 50721 2 . LEU 485 485 50721 2 . GLU 486 486 50721 2 . PHE 487 487 50721 2 . SER 488 488 50721 2 . ARG 489 489 50721 2 . ASP 490 490 50721 2 . ARG 491 491 50721 2 . LYS 492 492 50721 2 . SER 493 493 50721 2 . MET 494 494 50721 2 . SER 495 495 50721 2 . VAL 496 496 50721 2 . TYR 497 497 50721 2 . CYS 498 498 50721 2 . SER 499 499 50721 2 . PRO 500 500 50721 2 . ALA 501 501 50721 2 . LYS 502 502 50721 2 . SER 503 503 50721 2 . SER 504 504 50721 2 . ARG 505 505 50721 2 . ALA 506 506 50721 2 . ALA 507 507 50721 2 . VAL 508 508 50721 2 . GLY 509 509 50721 2 . ASN 510 510 50721 2 . LYS 511 511 50721 2 . MET 512 512 50721 2 . PHE 513 513 50721 2 . VAL 514 514 50721 2 . LYS 515 515 50721 2 . GLY 516 516 50721 2 . ALA 517 517 50721 2 . PRO 518 518 50721 2 . GLU 519 519 50721 2 . GLY 520 520 50721 2 . VAL 521 521 50721 2 . ILE 522 522 50721 2 . ASP 523 523 50721 2 . ARG 524 524 50721 2 . CYS 525 525 50721 2 . ASN 526 526 50721 2 . TYR 527 527 50721 2 . VAL 528 528 50721 2 . ARG 529 529 50721 2 . VAL 530 530 50721 2 . GLY 531 531 50721 2 . THR 532 532 50721 2 . THR 533 533 50721 2 . ARG 534 534 50721 2 . VAL 535 535 50721 2 . PRO 536 536 50721 2 . MET 537 537 50721 2 . THR 538 538 50721 2 . GLY 539 539 50721 2 . PRO 540 540 50721 2 . VAL 541 541 50721 2 . LYS 542 542 50721 2 . GLU 543 543 50721 2 . LYS 544 544 50721 2 . ILE 545 545 50721 2 . LEU 546 546 50721 2 . SER 547 547 50721 2 . VAL 548 548 50721 2 . ILE 549 549 50721 2 . LYS 550 550 50721 2 . GLU 551 551 50721 2 . TRP 552 552 50721 2 . GLY 553 553 50721 2 . THR 554 554 50721 2 . GLY 555 555 50721 2 . ARG 556 556 50721 2 . ASP 557 557 50721 2 . THR 558 558 50721 2 . LEU 559 559 50721 2 . ARG 560 560 50721 2 . CYS 561 561 50721 2 . LEU 562 562 50721 2 . ALA 563 563 50721 2 . LEU 564 564 50721 2 . ALA 565 565 50721 2 . THR 566 566 50721 2 . ARG 567 567 50721 2 . ASP 568 568 50721 2 . THR 569 569 50721 2 . PRO 570 570 50721 2 . PRO 571 571 50721 2 . LYS 572 572 50721 2 . ARG 573 573 50721 2 . GLU 574 574 50721 2 . GLU 575 575 50721 2 . MET 576 576 50721 2 . VAL 577 577 50721 2 . LEU 578 578 50721 2 . ASP 579 579 50721 2 . ASP 580 580 50721 2 . SER 581 581 50721 2 . SER 582 582 50721 2 . ARG 583 583 50721 2 . PHE 584 584 50721 2 . MET 585 585 50721 2 . GLU 586 586 50721 2 . TYR 587 587 50721 2 . GLU 588 588 50721 2 . THR 589 589 50721 2 . ASP 590 590 50721 2 . LEU 591 591 50721 2 . THR 592 592 50721 2 . PHE 593 593 50721 2 . VAL 594 594 50721 2 . GLY 595 595 50721 2 . VAL 596 596 50721 2 . VAL 597 597 50721 2 . GLY 598 598 50721 2 . MET 599 599 50721 2 . LEU 600 600 50721 2 . ASP 601 601 50721 2 . PRO 602 602 50721 2 . PRO 603 603 50721 2 . ARG 604 604 50721 2 . LYS 605 605 50721 2 . GLU 606 606 50721 2 . VAL 607 607 50721 2 . MET 608 608 50721 2 . GLY 609 609 50721 2 . SER 610 610 50721 2 . ILE 611 611 50721 2 . GLN 612 612 50721 2 . LEU 613 613 50721 2 . CYS 614 614 50721 2 . ARG 615 615 50721 2 . ASP 616 616 50721 2 . ALA 617 617 50721 2 . GLY 618 618 50721 2 . ILE 619 619 50721 2 . ARG 620 620 50721 2 . VAL 621 621 50721 2 . ILE 622 622 50721 2 . MET 623 623 50721 2 . ILE 624 624 50721 2 . THR 625 625 50721 2 . GLY 626 626 50721 2 . ASP 627 627 50721 2 . ASN 628 628 50721 2 . LYS 629 629 50721 2 . GLY 630 630 50721 2 . THR 631 631 50721 2 . ALA 632 632 50721 2 . ILE 633 633 50721 2 . ALA 634 634 50721 2 . ILE 635 635 50721 2 . CYS 636 636 50721 2 . ARG 637 637 50721 2 . ARG 638 638 50721 2 . ILE 639 639 50721 2 . GLY 640 640 50721 2 . ILE 641 641 50721 2 . PHE 642 642 50721 2 . GLY 643 643 50721 2 . GLU 644 644 50721 2 . ASN 645 645 50721 2 . GLU 646 646 50721 2 . GLU 647 647 50721 2 . VAL 648 648 50721 2 . ALA 649 649 50721 2 . ASP 650 650 50721 2 . ARG 651 651 50721 2 . ALA 652 652 50721 2 . TYR 653 653 50721 2 . THR 654 654 50721 2 . GLY 655 655 50721 2 . ARG 656 656 50721 2 . GLU 657 657 50721 2 . PHE 658 658 50721 2 . ASP 659 659 50721 2 . ASP 660 660 50721 2 . LEU 661 661 50721 2 . PRO 662 662 50721 2 . LEU 663 663 50721 2 . ALA 664 664 50721 2 . GLU 665 665 50721 2 . GLN 666 666 50721 2 . ARG 667 667 50721 2 . GLU 668 668 50721 2 . ALA 669 669 50721 2 . CYS 670 670 50721 2 . ARG 671 671 50721 2 . ARG 672 672 50721 2 . ALA 673 673 50721 2 . CYS 674 674 50721 2 . CYS 675 675 50721 2 . PHE 676 676 50721 2 . ALA 677 677 50721 2 . ARG 678 678 50721 2 . VAL 679 679 50721 2 . GLU 680 680 50721 2 . PRO 681 681 50721 2 . SER 682 682 50721 2 . HIS 683 683 50721 2 . LYS 684 684 50721 2 . SER 685 685 50721 2 . LYS 686 686 50721 2 . ILE 687 687 50721 2 . VAL 688 688 50721 2 . GLU 689 689 50721 2 . TYR 690 690 50721 2 . LEU 691 691 50721 2 . GLN 692 692 50721 2 . SER 693 693 50721 2 . TYR 694 694 50721 2 . ASP 695 695 50721 2 . GLU 696 696 50721 2 . ILE 697 697 50721 2 . THR 698 698 50721 2 . ALA 699 699 50721 2 . MET 700 700 50721 2 . THR 701 701 50721 2 . GLY 702 702 50721 2 . ASP 703 703 50721 2 . GLY 704 704 50721 2 . VAL 705 705 50721 2 . ASN 706 706 50721 2 . ASP 707 707 50721 2 . ALA 708 708 50721 2 . PRO 709 709 50721 2 . ALA 710 710 50721 2 . LEU 711 711 50721 2 . LYS 712 712 50721 2 . LYS 713 713 50721 2 . ALA 714 714 50721 2 . GLU 715 715 50721 2 . ILE 716 716 50721 2 . GLY 717 717 50721 2 . ILE 718 718 50721 2 . ALA 719 719 50721 2 . MET 720 720 50721 2 . GLY 721 721 50721 2 . SER 722 722 50721 2 . GLY 723 723 50721 2 . THR 724 724 50721 2 . ALA 725 725 50721 2 . VAL 726 726 50721 2 . ALA 727 727 50721 2 . LYS 728 728 50721 2 . THR 729 729 50721 2 . ALA 730 730 50721 2 . SER 731 731 50721 2 . GLU 732 732 50721 2 . MET 733 733 50721 2 . VAL 734 734 50721 2 . LEU 735 735 50721 2 . ALA 736 736 50721 2 . ASP 737 737 50721 2 . ASP 738 738 50721 2 . ASN 739 739 50721 2 . PHE 740 740 50721 2 . SER 741 741 50721 2 . THR 742 742 50721 2 . ILE 743 743 50721 2 . VAL 744 744 50721 2 . ALA 745 745 50721 2 . ALA 746 746 50721 2 . VAL 747 747 50721 2 . GLU 748 748 50721 2 . GLU 749 749 50721 2 . GLY 750 750 50721 2 . ARG 751 751 50721 2 . ALA 752 752 50721 2 . ILE 753 753 50721 2 . TYR 754 754 50721 2 . ASN 755 755 50721 2 . ASN 756 756 50721 2 . MET 757 757 50721 2 . LYS 758 758 50721 2 . GLN 759 759 50721 2 . PHE 760 760 50721 2 . ILE 761 761 50721 2 . ARG 762 762 50721 2 . TYR 763 763 50721 2 . LEU 764 764 50721 2 . ILE 765 765 50721 2 . SER 766 766 50721 2 . SER 767 767 50721 2 . ASN 768 768 50721 2 . VAL 769 769 50721 2 . GLY 770 770 50721 2 . GLU 771 771 50721 2 . VAL 772 772 50721 2 . VAL 773 773 50721 2 . CYS 774 774 50721 2 . ILE 775 775 50721 2 . PHE 776 776 50721 2 . LEU 777 777 50721 2 . THR 778 778 50721 2 . ALA 779 779 50721 2 . ALA 780 780 50721 2 . LEU 781 781 50721 2 . GLY 782 782 50721 2 . LEU 783 783 50721 2 . PRO 784 784 50721 2 . GLU 785 785 50721 2 . ALA 786 786 50721 2 . LEU 787 787 50721 2 . ILE 788 788 50721 2 . PRO 789 789 50721 2 . VAL 790 790 50721 2 . GLN 791 791 50721 2 . LEU 792 792 50721 2 . LEU 793 793 50721 2 . TRP 794 794 50721 2 . VAL 795 795 50721 2 . ASN 796 796 50721 2 . LEU 797 797 50721 2 . VAL 798 798 50721 2 . THR 799 799 50721 2 . ASP 800 800 50721 2 . GLY 801 801 50721 2 . LEU 802 802 50721 2 . PRO 803 803 50721 2 . ALA 804 804 50721 2 . THR 805 805 50721 2 . ALA 806 806 50721 2 . LEU 807 807 50721 2 . GLY 808 808 50721 2 . PHE 809 809 50721 2 . ASN 810 810 50721 2 . PRO 811 811 50721 2 . PRO 812 812 50721 2 . ASP 813 813 50721 2 . LEU 814 814 50721 2 . ASP 815 815 50721 2 . ILE 816 816 50721 2 . MET 817 817 50721 2 . ASP 818 818 50721 2 . ARG 819 819 50721 2 . PRO 820 820 50721 2 . PRO 821 821 50721 2 . ARG 822 822 50721 2 . SER 823 823 50721 2 . PRO 824 824 50721 2 . LYS 825 825 50721 2 . GLU 826 826 50721 2 . PRO 827 827 50721 2 . LEU 828 828 50721 2 . ILE 829 829 50721 2 . SER 830 830 50721 2 . GLY 831 831 50721 2 . TRP 832 832 50721 2 . LEU 833 833 50721 2 . PHE 834 834 50721 2 . PHE 835 835 50721 2 . ARG 836 836 50721 2 . TYR 837 837 50721 2 . MET 838 838 50721 2 . ALA 839 839 50721 2 . ILE 840 840 50721 2 . GLY 841 841 50721 2 . GLY 842 842 50721 2 . TYR 843 843 50721 2 . VAL 844 844 50721 2 . GLY 845 845 50721 2 . ALA 846 846 50721 2 . ALA 847 847 50721 2 . THR 848 848 50721 2 . VAL 849 849 50721 2 . GLY 850 850 50721 2 . ALA 851 851 50721 2 . ALA 852 852 50721 2 . ALA 853 853 50721 2 . TRP 854 854 50721 2 . TRP 855 855 50721 2 . PHE 856 856 50721 2 . MET 857 857 50721 2 . TYR 858 858 50721 2 . ALA 859 859 50721 2 . GLU 860 860 50721 2 . ASP 861 861 50721 2 . GLY 862 862 50721 2 . PRO 863 863 50721 2 . GLY 864 864 50721 2 . VAL 865 865 50721 2 . THR 866 866 50721 2 . TYR 867 867 50721 2 . HIS 868 868 50721 2 . GLN 869 869 50721 2 . LEU 870 870 50721 2 . THR 871 871 50721 2 . HIS 872 872 50721 2 . PHE 873 873 50721 2 . MET 874 874 50721 2 . GLN 875 875 50721 2 . CYS 876 876 50721 2 . THR 877 877 50721 2 . GLU 878 878 50721 2 . ASP 879 879 50721 2 . HIS 880 880 50721 2 . PRO 881 881 50721 2 . HIS 882 882 50721 2 . PHE 883 883 50721 2 . GLU 884 884 50721 2 . GLY 885 885 50721 2 . LEU 886 886 50721 2 . ASP 887 887 50721 2 . CYS 888 888 50721 2 . GLU 889 889 50721 2 . ILE 890 890 50721 2 . PHE 891 891 50721 2 . GLU 892 892 50721 2 . ALA 893 893 50721 2 . PRO 894 894 50721 2 . GLU 895 895 50721 2 . PRO 896 896 50721 2 . MET 897 897 50721 2 . THR 898 898 50721 2 . MET 899 899 50721 2 . ALA 900 900 50721 2 . LEU 901 901 50721 2 . SER 902 902 50721 2 . VAL 903 903 50721 2 . LEU 904 904 50721 2 . VAL 905 905 50721 2 . THR 906 906 50721 2 . ILE 907 907 50721 2 . GLU 908 908 50721 2 . MET 909 909 50721 2 . CYS 910 910 50721 2 . ASN 911 911 50721 2 . ALA 912 912 50721 2 . LEU 913 913 50721 2 . ASN 914 914 50721 2 . SER 915 915 50721 2 . LEU 916 916 50721 2 . SER 917 917 50721 2 . GLU 918 918 50721 2 . ASN 919 919 50721 2 . GLN 920 920 50721 2 . SER 921 921 50721 2 . LEU 922 922 50721 2 . MET 923 923 50721 2 . ARG 924 924 50721 2 . MET 925 925 50721 2 . PRO 926 926 50721 2 . PRO 927 927 50721 2 . TRP 928 928 50721 2 . VAL 929 929 50721 2 . ASN 930 930 50721 2 . ILE 931 931 50721 2 . TRP 932 932 50721 2 . LEU 933 933 50721 2 . LEU 934 934 50721 2 . GLY 935 935 50721 2 . SER 936 936 50721 2 . ILE 937 937 50721 2 . CYS 938 938 50721 2 . LEU 939 939 50721 2 . SER 940 940 50721 2 . MET 941 941 50721 2 . SER 942 942 50721 2 . LEU 943 943 50721 2 . HIS 944 944 50721 2 . PHE 945 945 50721 2 . LEU 946 946 50721 2 . ILE 947 947 50721 2 . LEU 948 948 50721 2 . TYR 949 949 50721 2 . VAL 950 950 50721 2 . ASP 951 951 50721 2 . PRO 952 952 50721 2 . LEU 953 953 50721 2 . PRO 954 954 50721 2 . MET 955 955 50721 2 . ILE 956 956 50721 2 . PHE 957 957 50721 2 . LYS 958 958 50721 2 . LEU 959 959 50721 2 . LYS 960 960 50721 2 . ALA 961 961 50721 2 . LEU 962 962 50721 2 . ASP 963 963 50721 2 . LEU 964 964 50721 2 . THR 965 965 50721 2 . GLN 966 966 50721 2 . TRP 967 967 50721 2 . LEU 968 968 50721 2 . MET 969 969 50721 2 . VAL 970 970 50721 2 . LEU 971 971 50721 2 . LYS 972 972 50721 2 . ILE 973 973 50721 2 . SER 974 974 50721 2 . LEU 975 975 50721 2 . PRO 976 976 50721 2 . VAL 977 977 50721 2 . ILE 978 978 50721 2 . GLY 979 979 50721 2 . LEU 980 980 50721 2 . ASP 981 981 50721 2 . GLU 982 982 50721 2 . ILE 983 983 50721 2 . LEU 984 984 50721 2 . LYS 985 985 50721 2 . PHE 986 986 50721 2 . ILE 987 987 50721 2 . ALA 988 988 50721 2 . ARG 989 989 50721 2 . ASN 990 990 50721 2 . TYR 991 991 50721 2 . LEU 992 992 50721 2 . GLU 993 993 50721 2 . ASP 994 994 50721 2 . PRO 995 995 50721 2 . GLU 996 996 50721 2 . ASP 997 997 50721 2 . GLU 998 998 50721 2 . ARG 999 999 50721 2 . ARG 1000 1000 50721 2 . LYS 1001 1001 50721 2 stop_ save_ save_entity_CA _Entity.Sf_category entity _Entity.Sf_framecode entity_CA _Entity.Entry_ID 50721 _Entity.ID 3 _Entity.BMRB_code CA _Entity.Name entity_CA _Entity.Type non-polymer _Entity.Polymer_common_type . _Entity.Polymer_type . _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code . _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states . _Entity.Ambiguous_chem_comp_sites . _Entity.Nstd_monomer . _Entity.Nstd_chirality . _Entity.Nstd_linkage . _Entity.Nonpolymer_comp_ID CA _Entity.Nonpolymer_comp_label $chem_comp_CA _Entity.Number_of_monomers . _Entity.Number_of_nonpolymer_components 1 _Entity.Paramagnetic . _Entity.Thiol_state . _Entity.Src_method . _Entity.Parent_entity_ID 3 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 40.078 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_common_name.Name _Entity_common_name.Type _Entity_common_name.Entry_ID _Entity_common_name.Entity_ID 'CALCIUM ION' BMRB 50721 3 stop_ loop_ _Entity_systematic_name.Name _Entity_systematic_name.Naming_system _Entity_systematic_name.Entry_ID _Entity_systematic_name.Entity_ID 'CALCIUM ION' BMRB 50721 3 CA 'Three letter code' 50721 3 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 1 CA $chem_comp_CA 50721 3 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 50721 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9986 organism . 'Oryctolagus cuniculus' rabbit . . Eukaryota Metazoa Oryctolagus cuniculus . . . . . . . . . . . . . 50721 1 2 2 $entity_2 . 9986 organism . 'Oryctolagus cuniculus' rabbit . . Eukaryota Metazoa Oryctolagus cuniculus . . . . . . . . . . . . . 50721 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 50721 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pMal-c2x . . . 50721 1 2 2 $entity_2 . 'purified from the natural source' . . . . . . . . . plasmid . . . . . . 50721 1 stop_ save_ ################################# # Polymer residues and ligands # ################################# save_chem_comp_CA _Chem_comp.Sf_category chem_comp _Chem_comp.Sf_framecode chem_comp_CA _Chem_comp.Entry_ID 50721 _Chem_comp.ID CA _Chem_comp.Provenance PDB _Chem_comp.Name 'CALCIUM ION' _Chem_comp.Type NON-POLYMER _Chem_comp.BMRB_code CA _Chem_comp.PDB_code CA _Chem_comp.Ambiguous_flag no _Chem_comp.Initial_date 2020-07-10 _Chem_comp.Modified_date 2020-07-10 _Chem_comp.Release_status REL _Chem_comp.Replaced_by . _Chem_comp.Replaces . _Chem_comp.One_letter_code . _Chem_comp.Three_letter_code CA _Chem_comp.Number_atoms_all 1 _Chem_comp.Number_atoms_nh 1 _Chem_comp.Atom_nomenclature_source . _Chem_comp.PubChem_code . _Chem_comp.Subcomponent_list . _Chem_comp.InChI_code InChI=1S/Ca/q+2 _Chem_comp.Mon_nstd_flag no _Chem_comp.Mon_nstd_class . _Chem_comp.Mon_nstd_details . _Chem_comp.Mon_nstd_parent . _Chem_comp.Mon_nstd_parent_comp_ID . _Chem_comp.Std_deriv_one_letter_code . _Chem_comp.Std_deriv_three_letter_code . _Chem_comp.Std_deriv_BMRB_code . _Chem_comp.Std_deriv_PDB_code . _Chem_comp.Std_deriv_chem_comp_name . _Chem_comp.Synonyms . _Chem_comp.Formal_charge 2 _Chem_comp.Paramagnetic . _Chem_comp.Aromatic no _Chem_comp.Formula Ca _Chem_comp.Formula_weight 40.078 _Chem_comp.Formula_mono_iso_wt_nat . _Chem_comp.Formula_mono_iso_wt_13C . _Chem_comp.Formula_mono_iso_wt_15N . _Chem_comp.Formula_mono_iso_wt_13C_15N . _Chem_comp.Image_file_name . _Chem_comp.Image_file_format . _Chem_comp.Topo_file_name . _Chem_comp.Topo_file_format . _Chem_comp.Struct_file_name . _Chem_comp.Struct_file_format . _Chem_comp.Stereochem_param_file_name . _Chem_comp.Stereochem_param_file_format . _Chem_comp.Model_details . _Chem_comp.Model_erf . _Chem_comp.Model_source . _Chem_comp.Model_coordinates_details . _Chem_comp.Model_coordinates_missing_flag no _Chem_comp.Ideal_coordinates_details . _Chem_comp.Ideal_coordinates_missing_flag no _Chem_comp.Model_coordinates_db_code . _Chem_comp.Processing_site RCSB _Chem_comp.Vendor . _Chem_comp.Vendor_product_code . _Chem_comp.Details . _Chem_comp.DB_query_date . _Chem_comp.DB_last_query_revised_last_date . loop_ _Chem_comp_descriptor.Descriptor _Chem_comp_descriptor.Type _Chem_comp_descriptor.Program _Chem_comp_descriptor.Program_version _Chem_comp_descriptor.Entry_ID _Chem_comp_descriptor.Comp_ID BHPQYMZQTOCNFJ-UHFFFAOYSA-N InChIKey InChI 1.03 50721 CA InChI=1S/Ca/q+2 InChI InChI 1.03 50721 CA [Ca++] SMILES CACTVS 3.341 50721 CA [Ca++] SMILES_CANONICAL CACTVS 3.341 50721 CA [Ca+2] SMILES ACDLabs 10.04 50721 CA [Ca+2] SMILES 'OpenEye OEToolkits' 1.5.0 50721 CA [Ca+2] SMILES_CANONICAL 'OpenEye OEToolkits' 1.5.0 50721 CA stop_ loop_ _Chem_comp_identifier.Identifier _Chem_comp_identifier.Type _Chem_comp_identifier.Program _Chem_comp_identifier.Program_version _Chem_comp_identifier.Entry_ID _Chem_comp_identifier.Comp_ID calcium 'SYSTEMATIC NAME' ACDLabs 10.04 50721 CA 'calcium(+2) cation' 'SYSTEMATIC NAME' 'OpenEye OEToolkits' 1.5.0 50721 CA stop_ loop_ _Chem_comp_atom.Atom_ID _Chem_comp_atom.BMRB_code _Chem_comp_atom.PDB_atom_ID _Chem_comp_atom.Alt_atom_ID _Chem_comp_atom.Auth_atom_ID _Chem_comp_atom.Type_symbol _Chem_comp_atom.Isotope_number _Chem_comp_atom.Chirality _Chem_comp_atom.Stereo_config _Chem_comp_atom.Charge _Chem_comp_atom.Partial_charge _Chem_comp_atom.Oxidation_number _Chem_comp_atom.Unpaired_electron_number _Chem_comp_atom.Align _Chem_comp_atom.Aromatic_flag _Chem_comp_atom.Leaving_atom_flag _Chem_comp_atom.Substruct_code _Chem_comp_atom.Ionizable _Chem_comp_atom.Drawing_2D_coord_x _Chem_comp_atom.Drawing_2D_coord_y _Chem_comp_atom.Model_Cartn_x _Chem_comp_atom.Model_Cartn_x_esd _Chem_comp_atom.Model_Cartn_y _Chem_comp_atom.Model_Cartn_y_esd _Chem_comp_atom.Model_Cartn_z _Chem_comp_atom.Model_Cartn_z_esd _Chem_comp_atom.Model_Cartn_x_ideal _Chem_comp_atom.Model_Cartn_y_ideal _Chem_comp_atom.Model_Cartn_z_ideal _Chem_comp_atom.PDBX_ordinal _Chem_comp_atom.Details _Chem_comp_atom.Entry_ID _Chem_comp_atom.Comp_ID CA CA CA CA . CA . . N 2 . . . 0 N N . . . . 0.000 . 0.000 . 0.000 . 0.000 0.000 0.000 1 . 50721 CA stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 50721 _Sample.ID 1 _Sample.Name 'Monomeric phospholamban in complex with SERCA in oriented bicelles (calcium-bound E1 state)' _Sample.Type bicelle _Sample.Sub_type . _Sample.Details 'Flipped blicelles (q = 4)' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system H2O _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Phospholamban (PLN-AFA)' [U-15N] . . 1 $entity_1 . . 0.21 . . mM . . . . 50721 1 2 HEPES 'natural abundance' . . . . . . 20 . . mM . . . . 50721 1 3 'potassium chloride' 'natural abundance' . . . . . . 100 . . mM . . . . 50721 1 4 'sodium azide' 'natural abundance' . . . . . . 1 . . mM . . . . 50721 1 5 glycerol 'natural abundance' . . . . . . 2.5 . . % . . . . 50721 1 6 '1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)' 'natural abundance' . . . . . . 340 . . mM . . . . 50721 1 7 '1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC)' 'natural abundance' . . . . . . 85 . . mM . . . . 50721 1 8 '1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC)' 'natural abundance' . . . . . . 106 . . mM . . . . 50721 1 9 '1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (PE-DTPA)' 'natural abundance' . . . . . . 6.4 . . mM . . . . 50721 1 10 'Ytterbium(III) chloride' 'natural abundance' . . . . . . 5 . . mM . . . . 50721 1 11 'Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (SERCA1a)' 'natural abundance' . . 2 $entity_2 . . 0.23 . . mM . . . . 50721 1 12 DTT 'natural abundance' . . . . . . 2 . . mM . . . . 50721 1 13 'magnesium chloride' 'natural abundance' . . . . . . 5 . . mM . . . . 50721 1 14 'calcium Chloride' 'natural abundance' . . . . . . 5 . . mM . . . . 50721 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 50721 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Phospholamban in complex with SERCA' _Sample_condition_list.Details 'Monomeric phospholamban reconstituted with SERCA in bicelles.' loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 7 . pH 50721 1 pressure 1 . atm 50721 1 temperature 298.15 . K 50721 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 50721 _Software.ID 1 _Software.Type . _Software.Name NMRPipe _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 50721 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 50721 _Software.ID 2 _Software.Type . _Software.Name VNMRj _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 50721 2 stop_ save_ save_software_3 _Software.Sf_category software _Software.Sf_framecode software_3 _Software.Entry_ID 50721 _Software.ID 3 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'data analysis' . 50721 3 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 50721 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 'Varian VNMRS - 700 MHz' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model VXRS _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 700 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 50721 _Experiment_list.ID 1 _Experiment_list.Details 'Orientated 15N chemical shift correlations with 15N-1H dipolar couplings.' loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D NH SLF' yes . . . . . . . . . . . . 1 $sample_1 anisotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50721 1 stop_ loop_ _Experiment_file.Experiment_ID _Experiment_file.Experiment_name _Experiment_file.Name _Experiment_file.Type _Experiment_file.Content _Experiment_file.Directory_path _Experiment_file.Details _Experiment_file.Entry_ID _Experiment_file.Experiment_list_ID 1 '2D NH SLF' sample4_afa-serca-e1_rawdata.zip . 'NMR experiment directory' . . 50721 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 50721 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 15N _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID N 15 '[15N] ammonium chloride' nitrogen . . . . ppm 39.3 external direct 1 . . . . . 50721 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 50721 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'PLN-AFA with SERCA (E1) 15N Shifts' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details 'Anisotropic chemical shifts and dipolar couplings (Hz) measured using a PISEMA-like experiment (SE-SAMPI4).' _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D NH SLF' . . . 50721 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 3 $software_3 . . 50721 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 33 33 PHE N N 15 181.764 5 . . . . . . . 32 PHE N . 50721 1 2 . 1 . 1 36 36 PHE N N 15 176.173 5 . . . . . . . 35 PHE N . 50721 1 3 . 1 . 1 37 37 ALA N N 15 190.01 5 . . . . . . . 36 ALA N . 50721 1 4 . 1 . 1 38 38 LEU N N 15 145.636 5 . . . . . . . 37 LEU N . 50721 1 5 . 1 . 1 39 39 ILE N N 15 156.669 5 . . . . . . . 38 ILE N . 50721 1 6 . 1 . 1 40 40 LEU N N 15 194.505 5 . . . . . . . 39 LEU N . 50721 1 7 . 1 . 1 41 41 ILE N N 15 166.594 5 . . . . . . . 40 ILE N . 50721 1 8 . 1 . 1 48 48 ILE N N 15 179.483 5 . . . . . . . 47 ILE N . 50721 1 9 . 1 . 1 49 49 ILE N N 15 150.949 5 . . . . . . . 48 ILE N . 50721 1 10 . 1 . 1 51 51 MET N N 15 202.893 5 . . . . . . . 50 MET N . 50721 1 stop_ save_ ################################ # Dipolar coupling constants # ################################ save_dipolar_couplings_1 _Dipolar_coupling_list.Sf_category dipolar_couplings _Dipolar_coupling_list.Sf_framecode dipolar_couplings_1 _Dipolar_coupling_list.Entry_ID 50721 _Dipolar_coupling_list.ID 1 _Dipolar_coupling_list.Name 'PLN-AFA with SERCA (E1) 15N-1H Dipolar Couplings' _Dipolar_coupling_list.Sample_condition_list_ID 1 _Dipolar_coupling_list.Sample_condition_list_label $sample_conditions_1 _Dipolar_coupling_list.Spectrometer_frequency_1H 700 _Dipolar_coupling_list.Scaling_factor . _Dipolar_coupling_list.Fitting_procedure . _Dipolar_coupling_list.Details 'Anisotropic chemical shifts and dipolar couplings (Hz) measured using a PISEMA-like experiment (SE-SAMPI4).' _Dipolar_coupling_list.Text_data_format . _Dipolar_coupling_list.Text_data . loop_ _Dipolar_coupling_experiment.Experiment_ID _Dipolar_coupling_experiment.Experiment_name _Dipolar_coupling_experiment.Sample_ID _Dipolar_coupling_experiment.Sample_label _Dipolar_coupling_experiment.Sample_state _Dipolar_coupling_experiment.Entry_ID _Dipolar_coupling_experiment.Dipolar_coupling_list_ID 1 '2D NH SLF' . . . 50721 1 stop_ loop_ _Dipolar_coupling_software.Software_ID _Dipolar_coupling_software.Software_label _Dipolar_coupling_software.Method_ID _Dipolar_coupling_software.Method_label _Dipolar_coupling_software.Entry_ID _Dipolar_coupling_software.Dipolar_coupling_list_ID 3 $software_3 . . 50721 1 stop_ loop_ _Dipolar_coupling.ID _Dipolar_coupling.Dipolar_coupling_code _Dipolar_coupling.Assembly_atom_ID_1 _Dipolar_coupling.Entity_assembly_ID_1 _Dipolar_coupling.Entity_ID_1 _Dipolar_coupling.Comp_index_ID_1 _Dipolar_coupling.Seq_ID_1 _Dipolar_coupling.Comp_ID_1 _Dipolar_coupling.Atom_ID_1 _Dipolar_coupling.Atom_type_1 _Dipolar_coupling.Atom_isotope_number_1 _Dipolar_coupling.Ambiguity_code_1 _Dipolar_coupling.Assembly_atom_ID_2 _Dipolar_coupling.Entity_assembly_ID_2 _Dipolar_coupling.Entity_ID_2 _Dipolar_coupling.Comp_index_ID_2 _Dipolar_coupling.Seq_ID_2 _Dipolar_coupling.Comp_ID_2 _Dipolar_coupling.Atom_ID_2 _Dipolar_coupling.Atom_type_2 _Dipolar_coupling.Atom_isotope_number_2 _Dipolar_coupling.Ambiguity_code_2 _Dipolar_coupling.Val _Dipolar_coupling.Val_min _Dipolar_coupling.Val_max _Dipolar_coupling.Val_err _Dipolar_coupling.Principal_Euler_angle_alpha_val _Dipolar_coupling.Principal_Euler_angle_beta_val _Dipolar_coupling.Principal_Euler_angle_gamma_val _Dipolar_coupling.Resonance_ID_1 _Dipolar_coupling.Resonance_ID_2 _Dipolar_coupling.Auth_entity_assembly_ID_1 _Dipolar_coupling.Auth_seq_ID_1 _Dipolar_coupling.Auth_comp_ID_1 _Dipolar_coupling.Auth_atom_ID_1 _Dipolar_coupling.Auth_entity_assembly_ID_2 _Dipolar_coupling.Auth_seq_ID_2 _Dipolar_coupling.Auth_comp_ID_2 _Dipolar_coupling.Auth_atom_ID_2 _Dipolar_coupling.Entry_ID _Dipolar_coupling.Dipolar_coupling_list_ID 1 . . 1 1 33 33 PHE N N 15 . . 1 1 33 33 PHE H H 1 . 3772 . . 500 . . . . . . 32 PHE N . 32 PHE H 50721 1 2 . . 1 1 36 36 PHE N N 15 . . 1 1 36 36 PHE H H 1 . 2331 . . 500 . . . . . . 35 PHE N . 35 PHE H 50721 1 3 . . 1 1 37 37 ALA N N 15 . . 1 1 37 37 ALA H H 1 . 5739 . . 500 . . . . . . 36 ALA N . 36 ALA H 50721 1 4 . . 1 1 38 38 LEU N N 15 . . 1 1 38 38 LEU H H 1 . 4473 . . 500 . . . . . . 37 LEU N . 37 LEU H 50721 1 5 . . 1 1 39 39 ILE N N 15 . . 1 1 39 39 ILE H H 1 . 2028 . . 500 . . . . . . 38 ILE N . 38 ILE H 50721 1 6 . . 1 1 40 40 LEU N N 15 . . 1 1 40 40 LEU H H 1 . 3808 . . 500 . . . . . . 39 LEU N . 39 LEU H 50721 1 7 . . 1 1 41 41 ILE N N 15 . . 1 1 41 41 ILE H H 1 . 7123 . . 500 . . . . . . 40 ILE N . 40 ILE H 50721 1 8 . . 1 1 48 48 ILE N N 15 . . 1 1 48 48 ILE H H 1 . 7020 . . 500 . . . . . . 47 ILE N . 47 ILE H 50721 1 9 . . 1 1 49 49 ILE N N 15 . . 1 1 49 49 ILE H H 1 . 5273 . . 500 . . . . . . 48 ILE N . 48 ILE H 50721 1 10 . . 1 1 51 51 MET N N 15 . . 1 1 51 51 MET H H 1 . 4969 . . 500 . . . . . . 50 MET N . 50 MET H 50721 1 stop_ save_