data_50720 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 50720 _Entry.Title ; Phospholamban bound to SERCA in oriented bicelles (calcium-free E2 state) ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2021-01-18 _Entry.Accession_date 2021-01-18 _Entry.Last_release_date 2021-01-20 _Entry.Original_release_date 2021-01-20 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solid-state _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Daniel Weber . K. . 0000-0001-8400-767X 50720 2 Maximo Sanz-Hernandez . . . 0000-0001-8522-8730 50720 3 'Venkateswara Reddy' Uddigiri . . . . 50720 4 Songlin Wang . . . 0000-0002-7588-7377 50720 5 Erik Larsen . K. . . 50720 6 Tata Gopinath . . . . 50720 7 Martin Gustavsson . . . 0000-0002-3015-2043 50720 8 Razvan Cornea . . . 0000-0001-5739-0992 50720 9 David Thomas . D. . . 50720 10 Alfonso 'De Simone' . . . 0000-0001-8789-9546 50720 11 Gianluigi Veglia . . . 0000-0002-2795-6964 50720 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 50720 dipolar_couplings 1 50720 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 10 50720 'dipolar coupling values' 10 50720 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2021-05-18 . original BMRB . 50720 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 50718 'Monomeric phospholamban in oriented bicelles' 50720 BMRB 50719 'Monomeric phosphorylated phospholamban in oriented bicelles' 50720 BMRB 50721 'Phospholamban bound to SERCA in oriented bicelles (calcium-bound E1 state)' 50720 BMRB 50722 'Phosphorylated phospholamban bound to SERCA in oriented bicelles (calcium-free E2 state)' 50720 BMRB 50723 'Phosphorylated phospholamban bound to SERCA in oriented bicelles (calcium-bound E1 state)' 50720 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 50720 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 33978571 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Structural basis for allosteric control of the SERCA-Phospholamban membrane complex by Ca 2+ and phosphorylation ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev Elife _Citation.Journal_name_full . _Citation.Journal_volume 10 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first e66226 _Citation.Page_last e66226 _Citation.Year 2021 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Daniel Weber D. K. . . 50720 1 2 'U Venkateswara' Reddy U. V. . . 50720 1 3 Songlin Wang S. . . . 50720 1 4 Erik Larsen E. K. . . 50720 1 5 Tata Gopinath T. . . . 50720 1 6 Martin Gustavsson M. B. . . 50720 1 7 Razvan Cornea R. L. . . 50720 1 8 David Thomas D. D. . . 50720 1 9 Alfonso 'De Simone' A. . . . 50720 1 10 Gianluigi Veglia G. . . . 50720 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 50720 _Assembly.ID 1 _Assembly.Name 'Phospholamban bound to SERCA' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange yes _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass 6165.6 _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 PLN 1 $entity_1 . . yes native no no . . . 50720 1 2 SERCA 2 $entity_2 . . no native no no . . . 50720 1 stop_ loop_ _Assembly_db_link.Author_supplied _Assembly_db_link.Database_code _Assembly_db_link.Accession_code _Assembly_db_link.Entry_mol_code _Assembly_db_link.Entry_mol_name _Assembly_db_link.Entry_experimental_method _Assembly_db_link.Entry_structure_resolution _Assembly_db_link.Entry_relation_type _Assembly_db_link.Entry_details _Assembly_db_link.Entry_ID _Assembly_db_link.Assembly_ID yes UNP P04191 . . . . . . 50720 1 yes UNP P61015 . . . . . . 50720 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 50720 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; AMEKVQYLTRSAIRRASTIE MPQQARQNLQNLFINFALIL IFLLLIAIIVMLL ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq ; A0, M1, E2, K3, V4, Q5, Y6, L7, T8, R9, S10, A11, I12, R13, R14, A15, S16, T17, I18, E19, M20, P21, Q22, Q23, A24, R25, Q26, N27, L28, Q29, N30, L31, F32, I33, N34, F35, A36, L37, I38, L39, I40, F41, L42, L43, L44, I45, A46, I47, I48, V49, M50, L51, L52 ; _Entity.Polymer_author_seq_details ; Monomeric cysteine-null mutant (C36A/C41F/C46A). N-terminal Ala0 left over from cleavage of MBP fusion protein. ; _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 53 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation 'C36A, C41F, C46A' _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 6166 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 yes UNP P61015 . . . . . . . . . . . . . . . . 50720 1 2 yes PDB 2KB7 . . . . . . . . . . . . . . . . 50720 1 3 yes PDB 2LPF . . . . . . . . . . . . . . . . 50720 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 0 ALA . 50720 1 2 1 MET . 50720 1 3 2 GLU . 50720 1 4 3 LYS . 50720 1 5 4 VAL . 50720 1 6 5 GLN . 50720 1 7 6 TYR . 50720 1 8 7 LEU . 50720 1 9 8 THR . 50720 1 10 9 ARG . 50720 1 11 10 SER . 50720 1 12 11 ALA . 50720 1 13 12 ILE . 50720 1 14 13 ARG . 50720 1 15 14 ARG . 50720 1 16 15 ALA . 50720 1 17 16 SER . 50720 1 18 17 THR . 50720 1 19 18 ILE . 50720 1 20 19 GLU . 50720 1 21 20 MET . 50720 1 22 21 PRO . 50720 1 23 22 GLN . 50720 1 24 23 GLN . 50720 1 25 24 ALA . 50720 1 26 25 ARG . 50720 1 27 26 GLN . 50720 1 28 27 ASN . 50720 1 29 28 LEU . 50720 1 30 29 GLN . 50720 1 31 30 ASN . 50720 1 32 31 LEU . 50720 1 33 32 PHE . 50720 1 34 33 ILE . 50720 1 35 34 ASN . 50720 1 36 35 PHE . 50720 1 37 36 ALA . 50720 1 38 37 LEU . 50720 1 39 38 ILE . 50720 1 40 39 LEU . 50720 1 41 40 ILE . 50720 1 42 41 PHE . 50720 1 43 42 LEU . 50720 1 44 43 LEU . 50720 1 45 44 LEU . 50720 1 46 45 ILE . 50720 1 47 46 ALA . 50720 1 48 47 ILE . 50720 1 49 48 ILE . 50720 1 50 49 VAL . 50720 1 51 50 MET . 50720 1 52 51 LEU . 50720 1 53 52 LEU . 50720 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ALA 1 1 50720 1 . MET 2 2 50720 1 . GLU 3 3 50720 1 . LYS 4 4 50720 1 . VAL 5 5 50720 1 . GLN 6 6 50720 1 . TYR 7 7 50720 1 . LEU 8 8 50720 1 . THR 9 9 50720 1 . ARG 10 10 50720 1 . SER 11 11 50720 1 . ALA 12 12 50720 1 . ILE 13 13 50720 1 . ARG 14 14 50720 1 . ARG 15 15 50720 1 . ALA 16 16 50720 1 . SER 17 17 50720 1 . THR 18 18 50720 1 . ILE 19 19 50720 1 . GLU 20 20 50720 1 . MET 21 21 50720 1 . PRO 22 22 50720 1 . GLN 23 23 50720 1 . GLN 24 24 50720 1 . ALA 25 25 50720 1 . ARG 26 26 50720 1 . GLN 27 27 50720 1 . ASN 28 28 50720 1 . LEU 29 29 50720 1 . GLN 30 30 50720 1 . ASN 31 31 50720 1 . LEU 32 32 50720 1 . PHE 33 33 50720 1 . ILE 34 34 50720 1 . ASN 35 35 50720 1 . PHE 36 36 50720 1 . ALA 37 37 50720 1 . LEU 38 38 50720 1 . ILE 39 39 50720 1 . LEU 40 40 50720 1 . ILE 41 41 50720 1 . PHE 42 42 50720 1 . LEU 43 43 50720 1 . LEU 44 44 50720 1 . LEU 45 45 50720 1 . ILE 46 46 50720 1 . ALA 47 47 50720 1 . ILE 48 48 50720 1 . ILE 49 49 50720 1 . VAL 50 50 50720 1 . MET 51 51 50720 1 . LEU 52 52 50720 1 . LEU 53 53 50720 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 50720 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name entity_2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MEAAHSKSTEECLAYFGVSE TTGLTPDQVKRHLEKYGHNE LPAEEGKSLWELVIEQFEDL LVRILLLAACISFVLAWFEE GEETITAFVEPFVILLILIA NAIVGVWQERNAENAIEALK EYEPEMGKVYRADRKSVQRI KARDIVPGDIVEVAVGDKVP ADIRILSIKSTTLRVDQSIL TGESVSVIKHTEPVPDPRAV NQDKKNMLFSGTNIAAGKAL GIVATTGVSTEIGKIRDQMA ATEQDKTPLQQKLDEFGEQL SKVISLICVAVWLINIGHFN DPVHGGSWIRGAIYYFKIAV ALAVAAIPEGLPAVITTCLA LGTRRMAKKNAIVRSLPSVE TLGCTSVICSDKTGTLTTNQ MSVCKMFIIDKVDGDFCSLN EFSITGSTYAPEGEVLKNDK PIRSGQFDGLVELATICALC NDSSLDFNETKGVYEKVGEA TETALTTLVEKMNVFNTEVR NLSKVERANACNSVIRQLMK KEFTLEFSRDRKSMSVYCSP AKSSRAAVGNKMFVKGAPEG VIDRCNYVRVGTTRVPMTGP VKEKILSVIKEWGTGRDTLR CLALATRDTPPKREEMVLDD SSRFMEYETDLTFVGVVGML DPPRKEVMGSIQLCRDAGIR VIMITGDNKGTAIAICRRIG IFGENEEVADRAYTGREFDD LPLAEQREACRRACCFARVE PSHKSKIVEYLQSYDEITAM TGDGVNDAPALKKAEIGIAM GSGTAVAKTASEMVLADDNF STIVAAVEEGRAIYNNMKQF IRYLISSNVGEVVCIFLTAA LGLPEALIPVQLLWVNLVTD GLPATALGFNPPDLDIMDRP PRSPKEPLISGWLFFRYMAI GGYVGAATVGAAAWWFMYAE DGPGVTYHQLTHFMQCTEDH PHFEGLDCEIFEAPEPMTMA LSVLVTIEMCNALNSLSENQ SLMRMPPWVNIWLLGSICLS MSLHFLILYVDPLPMIFKLK ALDLTQWLMVLKISLPVIGL DEILKFIARNYLEDPEDERR K ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 1001 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 yes UNP P04191 . . . . . . . . . . . . . . . . 50720 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 50720 2 2 . GLU . 50720 2 3 . ALA . 50720 2 4 . ALA . 50720 2 5 . HIS . 50720 2 6 . SER . 50720 2 7 . LYS . 50720 2 8 . SER . 50720 2 9 . THR . 50720 2 10 . GLU . 50720 2 11 . GLU . 50720 2 12 . CYS . 50720 2 13 . LEU . 50720 2 14 . ALA . 50720 2 15 . TYR . 50720 2 16 . PHE . 50720 2 17 . GLY . 50720 2 18 . VAL . 50720 2 19 . SER . 50720 2 20 . GLU . 50720 2 21 . THR . 50720 2 22 . THR . 50720 2 23 . GLY . 50720 2 24 . LEU . 50720 2 25 . THR . 50720 2 26 . PRO . 50720 2 27 . ASP . 50720 2 28 . GLN . 50720 2 29 . VAL . 50720 2 30 . LYS . 50720 2 31 . ARG . 50720 2 32 . HIS . 50720 2 33 . LEU . 50720 2 34 . GLU . 50720 2 35 . LYS . 50720 2 36 . TYR . 50720 2 37 . GLY . 50720 2 38 . HIS . 50720 2 39 . ASN . 50720 2 40 . GLU . 50720 2 41 . LEU . 50720 2 42 . PRO . 50720 2 43 . ALA . 50720 2 44 . GLU . 50720 2 45 . GLU . 50720 2 46 . GLY . 50720 2 47 . LYS . 50720 2 48 . SER . 50720 2 49 . LEU . 50720 2 50 . TRP . 50720 2 51 . GLU . 50720 2 52 . LEU . 50720 2 53 . VAL . 50720 2 54 . ILE . 50720 2 55 . GLU . 50720 2 56 . GLN . 50720 2 57 . PHE . 50720 2 58 . GLU . 50720 2 59 . ASP . 50720 2 60 . LEU . 50720 2 61 . LEU . 50720 2 62 . VAL . 50720 2 63 . ARG . 50720 2 64 . ILE . 50720 2 65 . LEU . 50720 2 66 . LEU . 50720 2 67 . LEU . 50720 2 68 . ALA . 50720 2 69 . ALA . 50720 2 70 . CYS . 50720 2 71 . ILE . 50720 2 72 . SER . 50720 2 73 . PHE . 50720 2 74 . VAL . 50720 2 75 . LEU . 50720 2 76 . ALA . 50720 2 77 . TRP . 50720 2 78 . PHE . 50720 2 79 . GLU . 50720 2 80 . GLU . 50720 2 81 . GLY . 50720 2 82 . GLU . 50720 2 83 . GLU . 50720 2 84 . THR . 50720 2 85 . ILE . 50720 2 86 . THR . 50720 2 87 . ALA . 50720 2 88 . PHE . 50720 2 89 . VAL . 50720 2 90 . GLU . 50720 2 91 . PRO . 50720 2 92 . PHE . 50720 2 93 . VAL . 50720 2 94 . ILE . 50720 2 95 . LEU . 50720 2 96 . LEU . 50720 2 97 . ILE . 50720 2 98 . LEU . 50720 2 99 . ILE . 50720 2 100 . ALA . 50720 2 101 . ASN . 50720 2 102 . ALA . 50720 2 103 . ILE . 50720 2 104 . VAL . 50720 2 105 . GLY . 50720 2 106 . VAL . 50720 2 107 . TRP . 50720 2 108 . GLN . 50720 2 109 . GLU . 50720 2 110 . ARG . 50720 2 111 . ASN . 50720 2 112 . ALA . 50720 2 113 . GLU . 50720 2 114 . ASN . 50720 2 115 . ALA . 50720 2 116 . ILE . 50720 2 117 . GLU . 50720 2 118 . ALA . 50720 2 119 . LEU . 50720 2 120 . LYS . 50720 2 121 . GLU . 50720 2 122 . TYR . 50720 2 123 . GLU . 50720 2 124 . PRO . 50720 2 125 . GLU . 50720 2 126 . MET . 50720 2 127 . GLY . 50720 2 128 . LYS . 50720 2 129 . VAL . 50720 2 130 . TYR . 50720 2 131 . ARG . 50720 2 132 . ALA . 50720 2 133 . ASP . 50720 2 134 . ARG . 50720 2 135 . LYS . 50720 2 136 . SER . 50720 2 137 . VAL . 50720 2 138 . GLN . 50720 2 139 . ARG . 50720 2 140 . ILE . 50720 2 141 . LYS . 50720 2 142 . ALA . 50720 2 143 . ARG . 50720 2 144 . ASP . 50720 2 145 . ILE . 50720 2 146 . VAL . 50720 2 147 . PRO . 50720 2 148 . GLY . 50720 2 149 . ASP . 50720 2 150 . ILE . 50720 2 151 . VAL . 50720 2 152 . GLU . 50720 2 153 . VAL . 50720 2 154 . ALA . 50720 2 155 . VAL . 50720 2 156 . GLY . 50720 2 157 . ASP . 50720 2 158 . LYS . 50720 2 159 . VAL . 50720 2 160 . PRO . 50720 2 161 . ALA . 50720 2 162 . ASP . 50720 2 163 . ILE . 50720 2 164 . ARG . 50720 2 165 . ILE . 50720 2 166 . LEU . 50720 2 167 . SER . 50720 2 168 . ILE . 50720 2 169 . LYS . 50720 2 170 . SER . 50720 2 171 . THR . 50720 2 172 . THR . 50720 2 173 . LEU . 50720 2 174 . ARG . 50720 2 175 . VAL . 50720 2 176 . ASP . 50720 2 177 . GLN . 50720 2 178 . SER . 50720 2 179 . ILE . 50720 2 180 . LEU . 50720 2 181 . THR . 50720 2 182 . GLY . 50720 2 183 . GLU . 50720 2 184 . SER . 50720 2 185 . VAL . 50720 2 186 . SER . 50720 2 187 . VAL . 50720 2 188 . ILE . 50720 2 189 . LYS . 50720 2 190 . HIS . 50720 2 191 . THR . 50720 2 192 . GLU . 50720 2 193 . PRO . 50720 2 194 . VAL . 50720 2 195 . PRO . 50720 2 196 . ASP . 50720 2 197 . PRO . 50720 2 198 . ARG . 50720 2 199 . ALA . 50720 2 200 . VAL . 50720 2 201 . ASN . 50720 2 202 . GLN . 50720 2 203 . ASP . 50720 2 204 . LYS . 50720 2 205 . LYS . 50720 2 206 . ASN . 50720 2 207 . MET . 50720 2 208 . LEU . 50720 2 209 . PHE . 50720 2 210 . SER . 50720 2 211 . GLY . 50720 2 212 . THR . 50720 2 213 . ASN . 50720 2 214 . ILE . 50720 2 215 . ALA . 50720 2 216 . ALA . 50720 2 217 . GLY . 50720 2 218 . LYS . 50720 2 219 . ALA . 50720 2 220 . LEU . 50720 2 221 . GLY . 50720 2 222 . ILE . 50720 2 223 . VAL . 50720 2 224 . ALA . 50720 2 225 . THR . 50720 2 226 . THR . 50720 2 227 . GLY . 50720 2 228 . VAL . 50720 2 229 . SER . 50720 2 230 . THR . 50720 2 231 . GLU . 50720 2 232 . ILE . 50720 2 233 . GLY . 50720 2 234 . LYS . 50720 2 235 . ILE . 50720 2 236 . ARG . 50720 2 237 . ASP . 50720 2 238 . GLN . 50720 2 239 . MET . 50720 2 240 . ALA . 50720 2 241 . ALA . 50720 2 242 . THR . 50720 2 243 . GLU . 50720 2 244 . GLN . 50720 2 245 . ASP . 50720 2 246 . LYS . 50720 2 247 . THR . 50720 2 248 . PRO . 50720 2 249 . LEU . 50720 2 250 . GLN . 50720 2 251 . GLN . 50720 2 252 . LYS . 50720 2 253 . LEU . 50720 2 254 . ASP . 50720 2 255 . GLU . 50720 2 256 . PHE . 50720 2 257 . GLY . 50720 2 258 . GLU . 50720 2 259 . GLN . 50720 2 260 . LEU . 50720 2 261 . SER . 50720 2 262 . LYS . 50720 2 263 . VAL . 50720 2 264 . ILE . 50720 2 265 . SER . 50720 2 266 . LEU . 50720 2 267 . ILE . 50720 2 268 . CYS . 50720 2 269 . VAL . 50720 2 270 . ALA . 50720 2 271 . VAL . 50720 2 272 . TRP . 50720 2 273 . LEU . 50720 2 274 . ILE . 50720 2 275 . ASN . 50720 2 276 . ILE . 50720 2 277 . GLY . 50720 2 278 . HIS . 50720 2 279 . PHE . 50720 2 280 . ASN . 50720 2 281 . ASP . 50720 2 282 . PRO . 50720 2 283 . VAL . 50720 2 284 . HIS . 50720 2 285 . GLY . 50720 2 286 . GLY . 50720 2 287 . SER . 50720 2 288 . TRP . 50720 2 289 . ILE . 50720 2 290 . ARG . 50720 2 291 . GLY . 50720 2 292 . ALA . 50720 2 293 . ILE . 50720 2 294 . TYR . 50720 2 295 . TYR . 50720 2 296 . PHE . 50720 2 297 . LYS . 50720 2 298 . ILE . 50720 2 299 . ALA . 50720 2 300 . VAL . 50720 2 301 . ALA . 50720 2 302 . LEU . 50720 2 303 . ALA . 50720 2 304 . VAL . 50720 2 305 . ALA . 50720 2 306 . ALA . 50720 2 307 . ILE . 50720 2 308 . PRO . 50720 2 309 . GLU . 50720 2 310 . GLY . 50720 2 311 . LEU . 50720 2 312 . PRO . 50720 2 313 . ALA . 50720 2 314 . VAL . 50720 2 315 . ILE . 50720 2 316 . THR . 50720 2 317 . THR . 50720 2 318 . CYS . 50720 2 319 . LEU . 50720 2 320 . ALA . 50720 2 321 . LEU . 50720 2 322 . GLY . 50720 2 323 . THR . 50720 2 324 . ARG . 50720 2 325 . ARG . 50720 2 326 . MET . 50720 2 327 . ALA . 50720 2 328 . LYS . 50720 2 329 . LYS . 50720 2 330 . ASN . 50720 2 331 . ALA . 50720 2 332 . ILE . 50720 2 333 . VAL . 50720 2 334 . ARG . 50720 2 335 . SER . 50720 2 336 . LEU . 50720 2 337 . PRO . 50720 2 338 . SER . 50720 2 339 . VAL . 50720 2 340 . GLU . 50720 2 341 . THR . 50720 2 342 . LEU . 50720 2 343 . GLY . 50720 2 344 . CYS . 50720 2 345 . THR . 50720 2 346 . SER . 50720 2 347 . VAL . 50720 2 348 . ILE . 50720 2 349 . CYS . 50720 2 350 . SER . 50720 2 351 . ASP . 50720 2 352 . LYS . 50720 2 353 . THR . 50720 2 354 . GLY . 50720 2 355 . THR . 50720 2 356 . LEU . 50720 2 357 . THR . 50720 2 358 . THR . 50720 2 359 . ASN . 50720 2 360 . GLN . 50720 2 361 . MET . 50720 2 362 . SER . 50720 2 363 . VAL . 50720 2 364 . CYS . 50720 2 365 . LYS . 50720 2 366 . MET . 50720 2 367 . PHE . 50720 2 368 . ILE . 50720 2 369 . ILE . 50720 2 370 . ASP . 50720 2 371 . LYS . 50720 2 372 . VAL . 50720 2 373 . ASP . 50720 2 374 . GLY . 50720 2 375 . ASP . 50720 2 376 . PHE . 50720 2 377 . CYS . 50720 2 378 . SER . 50720 2 379 . LEU . 50720 2 380 . ASN . 50720 2 381 . GLU . 50720 2 382 . PHE . 50720 2 383 . SER . 50720 2 384 . ILE . 50720 2 385 . THR . 50720 2 386 . GLY . 50720 2 387 . SER . 50720 2 388 . THR . 50720 2 389 . TYR . 50720 2 390 . ALA . 50720 2 391 . PRO . 50720 2 392 . GLU . 50720 2 393 . GLY . 50720 2 394 . GLU . 50720 2 395 . VAL . 50720 2 396 . LEU . 50720 2 397 . LYS . 50720 2 398 . ASN . 50720 2 399 . ASP . 50720 2 400 . LYS . 50720 2 401 . PRO . 50720 2 402 . ILE . 50720 2 403 . ARG . 50720 2 404 . SER . 50720 2 405 . GLY . 50720 2 406 . GLN . 50720 2 407 . PHE . 50720 2 408 . ASP . 50720 2 409 . GLY . 50720 2 410 . LEU . 50720 2 411 . VAL . 50720 2 412 . GLU . 50720 2 413 . LEU . 50720 2 414 . ALA . 50720 2 415 . THR . 50720 2 416 . ILE . 50720 2 417 . CYS . 50720 2 418 . ALA . 50720 2 419 . LEU . 50720 2 420 . CYS . 50720 2 421 . ASN . 50720 2 422 . ASP . 50720 2 423 . SER . 50720 2 424 . SER . 50720 2 425 . LEU . 50720 2 426 . ASP . 50720 2 427 . PHE . 50720 2 428 . ASN . 50720 2 429 . GLU . 50720 2 430 . THR . 50720 2 431 . LYS . 50720 2 432 . GLY . 50720 2 433 . VAL . 50720 2 434 . TYR . 50720 2 435 . GLU . 50720 2 436 . LYS . 50720 2 437 . VAL . 50720 2 438 . GLY . 50720 2 439 . GLU . 50720 2 440 . ALA . 50720 2 441 . THR . 50720 2 442 . GLU . 50720 2 443 . THR . 50720 2 444 . ALA . 50720 2 445 . LEU . 50720 2 446 . THR . 50720 2 447 . THR . 50720 2 448 . LEU . 50720 2 449 . VAL . 50720 2 450 . GLU . 50720 2 451 . LYS . 50720 2 452 . MET . 50720 2 453 . ASN . 50720 2 454 . VAL . 50720 2 455 . PHE . 50720 2 456 . ASN . 50720 2 457 . THR . 50720 2 458 . GLU . 50720 2 459 . VAL . 50720 2 460 . ARG . 50720 2 461 . ASN . 50720 2 462 . LEU . 50720 2 463 . SER . 50720 2 464 . LYS . 50720 2 465 . VAL . 50720 2 466 . GLU . 50720 2 467 . ARG . 50720 2 468 . ALA . 50720 2 469 . ASN . 50720 2 470 . ALA . 50720 2 471 . CYS . 50720 2 472 . ASN . 50720 2 473 . SER . 50720 2 474 . VAL . 50720 2 475 . ILE . 50720 2 476 . ARG . 50720 2 477 . GLN . 50720 2 478 . LEU . 50720 2 479 . MET . 50720 2 480 . LYS . 50720 2 481 . LYS . 50720 2 482 . GLU . 50720 2 483 . PHE . 50720 2 484 . THR . 50720 2 485 . LEU . 50720 2 486 . GLU . 50720 2 487 . PHE . 50720 2 488 . SER . 50720 2 489 . ARG . 50720 2 490 . ASP . 50720 2 491 . ARG . 50720 2 492 . LYS . 50720 2 493 . SER . 50720 2 494 . MET . 50720 2 495 . SER . 50720 2 496 . VAL . 50720 2 497 . TYR . 50720 2 498 . CYS . 50720 2 499 . SER . 50720 2 500 . PRO . 50720 2 501 . ALA . 50720 2 502 . LYS . 50720 2 503 . SER . 50720 2 504 . SER . 50720 2 505 . ARG . 50720 2 506 . ALA . 50720 2 507 . ALA . 50720 2 508 . VAL . 50720 2 509 . GLY . 50720 2 510 . ASN . 50720 2 511 . LYS . 50720 2 512 . MET . 50720 2 513 . PHE . 50720 2 514 . VAL . 50720 2 515 . LYS . 50720 2 516 . GLY . 50720 2 517 . ALA . 50720 2 518 . PRO . 50720 2 519 . GLU . 50720 2 520 . GLY . 50720 2 521 . VAL . 50720 2 522 . ILE . 50720 2 523 . ASP . 50720 2 524 . ARG . 50720 2 525 . CYS . 50720 2 526 . ASN . 50720 2 527 . TYR . 50720 2 528 . VAL . 50720 2 529 . ARG . 50720 2 530 . VAL . 50720 2 531 . GLY . 50720 2 532 . THR . 50720 2 533 . THR . 50720 2 534 . ARG . 50720 2 535 . VAL . 50720 2 536 . PRO . 50720 2 537 . MET . 50720 2 538 . THR . 50720 2 539 . GLY . 50720 2 540 . PRO . 50720 2 541 . VAL . 50720 2 542 . LYS . 50720 2 543 . GLU . 50720 2 544 . LYS . 50720 2 545 . ILE . 50720 2 546 . LEU . 50720 2 547 . SER . 50720 2 548 . VAL . 50720 2 549 . ILE . 50720 2 550 . LYS . 50720 2 551 . GLU . 50720 2 552 . TRP . 50720 2 553 . GLY . 50720 2 554 . THR . 50720 2 555 . GLY . 50720 2 556 . ARG . 50720 2 557 . ASP . 50720 2 558 . THR . 50720 2 559 . LEU . 50720 2 560 . ARG . 50720 2 561 . CYS . 50720 2 562 . LEU . 50720 2 563 . ALA . 50720 2 564 . LEU . 50720 2 565 . ALA . 50720 2 566 . THR . 50720 2 567 . ARG . 50720 2 568 . ASP . 50720 2 569 . THR . 50720 2 570 . PRO . 50720 2 571 . PRO . 50720 2 572 . LYS . 50720 2 573 . ARG . 50720 2 574 . GLU . 50720 2 575 . GLU . 50720 2 576 . MET . 50720 2 577 . VAL . 50720 2 578 . LEU . 50720 2 579 . ASP . 50720 2 580 . ASP . 50720 2 581 . SER . 50720 2 582 . SER . 50720 2 583 . ARG . 50720 2 584 . PHE . 50720 2 585 . MET . 50720 2 586 . GLU . 50720 2 587 . TYR . 50720 2 588 . GLU . 50720 2 589 . THR . 50720 2 590 . ASP . 50720 2 591 . LEU . 50720 2 592 . THR . 50720 2 593 . PHE . 50720 2 594 . VAL . 50720 2 595 . GLY . 50720 2 596 . VAL . 50720 2 597 . VAL . 50720 2 598 . GLY . 50720 2 599 . MET . 50720 2 600 . LEU . 50720 2 601 . ASP . 50720 2 602 . PRO . 50720 2 603 . PRO . 50720 2 604 . ARG . 50720 2 605 . LYS . 50720 2 606 . GLU . 50720 2 607 . VAL . 50720 2 608 . MET . 50720 2 609 . GLY . 50720 2 610 . SER . 50720 2 611 . ILE . 50720 2 612 . GLN . 50720 2 613 . LEU . 50720 2 614 . CYS . 50720 2 615 . ARG . 50720 2 616 . ASP . 50720 2 617 . ALA . 50720 2 618 . GLY . 50720 2 619 . ILE . 50720 2 620 . ARG . 50720 2 621 . VAL . 50720 2 622 . ILE . 50720 2 623 . MET . 50720 2 624 . ILE . 50720 2 625 . THR . 50720 2 626 . GLY . 50720 2 627 . ASP . 50720 2 628 . ASN . 50720 2 629 . LYS . 50720 2 630 . GLY . 50720 2 631 . THR . 50720 2 632 . ALA . 50720 2 633 . ILE . 50720 2 634 . ALA . 50720 2 635 . ILE . 50720 2 636 . CYS . 50720 2 637 . ARG . 50720 2 638 . ARG . 50720 2 639 . ILE . 50720 2 640 . GLY . 50720 2 641 . ILE . 50720 2 642 . PHE . 50720 2 643 . GLY . 50720 2 644 . GLU . 50720 2 645 . ASN . 50720 2 646 . GLU . 50720 2 647 . GLU . 50720 2 648 . VAL . 50720 2 649 . ALA . 50720 2 650 . ASP . 50720 2 651 . ARG . 50720 2 652 . ALA . 50720 2 653 . TYR . 50720 2 654 . THR . 50720 2 655 . GLY . 50720 2 656 . ARG . 50720 2 657 . GLU . 50720 2 658 . PHE . 50720 2 659 . ASP . 50720 2 660 . ASP . 50720 2 661 . LEU . 50720 2 662 . PRO . 50720 2 663 . LEU . 50720 2 664 . ALA . 50720 2 665 . GLU . 50720 2 666 . GLN . 50720 2 667 . ARG . 50720 2 668 . GLU . 50720 2 669 . ALA . 50720 2 670 . CYS . 50720 2 671 . ARG . 50720 2 672 . ARG . 50720 2 673 . ALA . 50720 2 674 . CYS . 50720 2 675 . CYS . 50720 2 676 . PHE . 50720 2 677 . ALA . 50720 2 678 . ARG . 50720 2 679 . VAL . 50720 2 680 . GLU . 50720 2 681 . PRO . 50720 2 682 . SER . 50720 2 683 . HIS . 50720 2 684 . LYS . 50720 2 685 . SER . 50720 2 686 . LYS . 50720 2 687 . ILE . 50720 2 688 . VAL . 50720 2 689 . GLU . 50720 2 690 . TYR . 50720 2 691 . LEU . 50720 2 692 . GLN . 50720 2 693 . SER . 50720 2 694 . TYR . 50720 2 695 . ASP . 50720 2 696 . GLU . 50720 2 697 . ILE . 50720 2 698 . THR . 50720 2 699 . ALA . 50720 2 700 . MET . 50720 2 701 . THR . 50720 2 702 . GLY . 50720 2 703 . ASP . 50720 2 704 . GLY . 50720 2 705 . VAL . 50720 2 706 . ASN . 50720 2 707 . ASP . 50720 2 708 . ALA . 50720 2 709 . PRO . 50720 2 710 . ALA . 50720 2 711 . LEU . 50720 2 712 . LYS . 50720 2 713 . LYS . 50720 2 714 . ALA . 50720 2 715 . GLU . 50720 2 716 . ILE . 50720 2 717 . GLY . 50720 2 718 . ILE . 50720 2 719 . ALA . 50720 2 720 . MET . 50720 2 721 . GLY . 50720 2 722 . SER . 50720 2 723 . GLY . 50720 2 724 . THR . 50720 2 725 . ALA . 50720 2 726 . VAL . 50720 2 727 . ALA . 50720 2 728 . LYS . 50720 2 729 . THR . 50720 2 730 . ALA . 50720 2 731 . SER . 50720 2 732 . GLU . 50720 2 733 . MET . 50720 2 734 . VAL . 50720 2 735 . LEU . 50720 2 736 . ALA . 50720 2 737 . ASP . 50720 2 738 . ASP . 50720 2 739 . ASN . 50720 2 740 . PHE . 50720 2 741 . SER . 50720 2 742 . THR . 50720 2 743 . ILE . 50720 2 744 . VAL . 50720 2 745 . ALA . 50720 2 746 . ALA . 50720 2 747 . VAL . 50720 2 748 . GLU . 50720 2 749 . GLU . 50720 2 750 . GLY . 50720 2 751 . ARG . 50720 2 752 . ALA . 50720 2 753 . ILE . 50720 2 754 . TYR . 50720 2 755 . ASN . 50720 2 756 . ASN . 50720 2 757 . MET . 50720 2 758 . LYS . 50720 2 759 . GLN . 50720 2 760 . PHE . 50720 2 761 . ILE . 50720 2 762 . ARG . 50720 2 763 . TYR . 50720 2 764 . LEU . 50720 2 765 . ILE . 50720 2 766 . SER . 50720 2 767 . SER . 50720 2 768 . ASN . 50720 2 769 . VAL . 50720 2 770 . GLY . 50720 2 771 . GLU . 50720 2 772 . VAL . 50720 2 773 . VAL . 50720 2 774 . CYS . 50720 2 775 . ILE . 50720 2 776 . PHE . 50720 2 777 . LEU . 50720 2 778 . THR . 50720 2 779 . ALA . 50720 2 780 . ALA . 50720 2 781 . LEU . 50720 2 782 . GLY . 50720 2 783 . LEU . 50720 2 784 . PRO . 50720 2 785 . GLU . 50720 2 786 . ALA . 50720 2 787 . LEU . 50720 2 788 . ILE . 50720 2 789 . PRO . 50720 2 790 . VAL . 50720 2 791 . GLN . 50720 2 792 . LEU . 50720 2 793 . LEU . 50720 2 794 . TRP . 50720 2 795 . VAL . 50720 2 796 . ASN . 50720 2 797 . LEU . 50720 2 798 . VAL . 50720 2 799 . THR . 50720 2 800 . ASP . 50720 2 801 . GLY . 50720 2 802 . LEU . 50720 2 803 . PRO . 50720 2 804 . ALA . 50720 2 805 . THR . 50720 2 806 . ALA . 50720 2 807 . LEU . 50720 2 808 . GLY . 50720 2 809 . PHE . 50720 2 810 . ASN . 50720 2 811 . PRO . 50720 2 812 . PRO . 50720 2 813 . ASP . 50720 2 814 . LEU . 50720 2 815 . ASP . 50720 2 816 . ILE . 50720 2 817 . MET . 50720 2 818 . ASP . 50720 2 819 . ARG . 50720 2 820 . PRO . 50720 2 821 . PRO . 50720 2 822 . ARG . 50720 2 823 . SER . 50720 2 824 . PRO . 50720 2 825 . LYS . 50720 2 826 . GLU . 50720 2 827 . PRO . 50720 2 828 . LEU . 50720 2 829 . ILE . 50720 2 830 . SER . 50720 2 831 . GLY . 50720 2 832 . TRP . 50720 2 833 . LEU . 50720 2 834 . PHE . 50720 2 835 . PHE . 50720 2 836 . ARG . 50720 2 837 . TYR . 50720 2 838 . MET . 50720 2 839 . ALA . 50720 2 840 . ILE . 50720 2 841 . GLY . 50720 2 842 . GLY . 50720 2 843 . TYR . 50720 2 844 . VAL . 50720 2 845 . GLY . 50720 2 846 . ALA . 50720 2 847 . ALA . 50720 2 848 . THR . 50720 2 849 . VAL . 50720 2 850 . GLY . 50720 2 851 . ALA . 50720 2 852 . ALA . 50720 2 853 . ALA . 50720 2 854 . TRP . 50720 2 855 . TRP . 50720 2 856 . PHE . 50720 2 857 . MET . 50720 2 858 . TYR . 50720 2 859 . ALA . 50720 2 860 . GLU . 50720 2 861 . ASP . 50720 2 862 . GLY . 50720 2 863 . PRO . 50720 2 864 . GLY . 50720 2 865 . VAL . 50720 2 866 . THR . 50720 2 867 . TYR . 50720 2 868 . HIS . 50720 2 869 . GLN . 50720 2 870 . LEU . 50720 2 871 . THR . 50720 2 872 . HIS . 50720 2 873 . PHE . 50720 2 874 . MET . 50720 2 875 . GLN . 50720 2 876 . CYS . 50720 2 877 . THR . 50720 2 878 . GLU . 50720 2 879 . ASP . 50720 2 880 . HIS . 50720 2 881 . PRO . 50720 2 882 . HIS . 50720 2 883 . PHE . 50720 2 884 . GLU . 50720 2 885 . GLY . 50720 2 886 . LEU . 50720 2 887 . ASP . 50720 2 888 . CYS . 50720 2 889 . GLU . 50720 2 890 . ILE . 50720 2 891 . PHE . 50720 2 892 . GLU . 50720 2 893 . ALA . 50720 2 894 . PRO . 50720 2 895 . GLU . 50720 2 896 . PRO . 50720 2 897 . MET . 50720 2 898 . THR . 50720 2 899 . MET . 50720 2 900 . ALA . 50720 2 901 . LEU . 50720 2 902 . SER . 50720 2 903 . VAL . 50720 2 904 . LEU . 50720 2 905 . VAL . 50720 2 906 . THR . 50720 2 907 . ILE . 50720 2 908 . GLU . 50720 2 909 . MET . 50720 2 910 . CYS . 50720 2 911 . ASN . 50720 2 912 . ALA . 50720 2 913 . LEU . 50720 2 914 . ASN . 50720 2 915 . SER . 50720 2 916 . LEU . 50720 2 917 . SER . 50720 2 918 . GLU . 50720 2 919 . ASN . 50720 2 920 . GLN . 50720 2 921 . SER . 50720 2 922 . LEU . 50720 2 923 . MET . 50720 2 924 . ARG . 50720 2 925 . MET . 50720 2 926 . PRO . 50720 2 927 . PRO . 50720 2 928 . TRP . 50720 2 929 . VAL . 50720 2 930 . ASN . 50720 2 931 . ILE . 50720 2 932 . TRP . 50720 2 933 . LEU . 50720 2 934 . LEU . 50720 2 935 . GLY . 50720 2 936 . SER . 50720 2 937 . ILE . 50720 2 938 . CYS . 50720 2 939 . LEU . 50720 2 940 . SER . 50720 2 941 . MET . 50720 2 942 . SER . 50720 2 943 . LEU . 50720 2 944 . HIS . 50720 2 945 . PHE . 50720 2 946 . LEU . 50720 2 947 . ILE . 50720 2 948 . LEU . 50720 2 949 . TYR . 50720 2 950 . VAL . 50720 2 951 . ASP . 50720 2 952 . PRO . 50720 2 953 . LEU . 50720 2 954 . PRO . 50720 2 955 . MET . 50720 2 956 . ILE . 50720 2 957 . PHE . 50720 2 958 . LYS . 50720 2 959 . LEU . 50720 2 960 . LYS . 50720 2 961 . ALA . 50720 2 962 . LEU . 50720 2 963 . ASP . 50720 2 964 . LEU . 50720 2 965 . THR . 50720 2 966 . GLN . 50720 2 967 . TRP . 50720 2 968 . LEU . 50720 2 969 . MET . 50720 2 970 . VAL . 50720 2 971 . LEU . 50720 2 972 . LYS . 50720 2 973 . ILE . 50720 2 974 . SER . 50720 2 975 . LEU . 50720 2 976 . PRO . 50720 2 977 . VAL . 50720 2 978 . ILE . 50720 2 979 . GLY . 50720 2 980 . LEU . 50720 2 981 . ASP . 50720 2 982 . GLU . 50720 2 983 . ILE . 50720 2 984 . LEU . 50720 2 985 . LYS . 50720 2 986 . PHE . 50720 2 987 . ILE . 50720 2 988 . ALA . 50720 2 989 . ARG . 50720 2 990 . ASN . 50720 2 991 . TYR . 50720 2 992 . LEU . 50720 2 993 . GLU . 50720 2 994 . ASP . 50720 2 995 . PRO . 50720 2 996 . GLU . 50720 2 997 . ASP . 50720 2 998 . GLU . 50720 2 999 . ARG . 50720 2 1000 . ARG . 50720 2 1001 . LYS . 50720 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 50720 2 . GLU 2 2 50720 2 . ALA 3 3 50720 2 . ALA 4 4 50720 2 . HIS 5 5 50720 2 . SER 6 6 50720 2 . LYS 7 7 50720 2 . SER 8 8 50720 2 . THR 9 9 50720 2 . GLU 10 10 50720 2 . GLU 11 11 50720 2 . CYS 12 12 50720 2 . LEU 13 13 50720 2 . ALA 14 14 50720 2 . TYR 15 15 50720 2 . PHE 16 16 50720 2 . GLY 17 17 50720 2 . VAL 18 18 50720 2 . SER 19 19 50720 2 . GLU 20 20 50720 2 . THR 21 21 50720 2 . THR 22 22 50720 2 . GLY 23 23 50720 2 . LEU 24 24 50720 2 . THR 25 25 50720 2 . PRO 26 26 50720 2 . ASP 27 27 50720 2 . GLN 28 28 50720 2 . VAL 29 29 50720 2 . LYS 30 30 50720 2 . ARG 31 31 50720 2 . HIS 32 32 50720 2 . LEU 33 33 50720 2 . GLU 34 34 50720 2 . LYS 35 35 50720 2 . TYR 36 36 50720 2 . GLY 37 37 50720 2 . HIS 38 38 50720 2 . ASN 39 39 50720 2 . GLU 40 40 50720 2 . LEU 41 41 50720 2 . PRO 42 42 50720 2 . ALA 43 43 50720 2 . GLU 44 44 50720 2 . GLU 45 45 50720 2 . GLY 46 46 50720 2 . LYS 47 47 50720 2 . SER 48 48 50720 2 . LEU 49 49 50720 2 . TRP 50 50 50720 2 . GLU 51 51 50720 2 . LEU 52 52 50720 2 . VAL 53 53 50720 2 . ILE 54 54 50720 2 . GLU 55 55 50720 2 . GLN 56 56 50720 2 . PHE 57 57 50720 2 . GLU 58 58 50720 2 . ASP 59 59 50720 2 . LEU 60 60 50720 2 . LEU 61 61 50720 2 . VAL 62 62 50720 2 . ARG 63 63 50720 2 . ILE 64 64 50720 2 . LEU 65 65 50720 2 . LEU 66 66 50720 2 . LEU 67 67 50720 2 . ALA 68 68 50720 2 . ALA 69 69 50720 2 . CYS 70 70 50720 2 . ILE 71 71 50720 2 . SER 72 72 50720 2 . PHE 73 73 50720 2 . VAL 74 74 50720 2 . LEU 75 75 50720 2 . ALA 76 76 50720 2 . TRP 77 77 50720 2 . PHE 78 78 50720 2 . GLU 79 79 50720 2 . GLU 80 80 50720 2 . GLY 81 81 50720 2 . GLU 82 82 50720 2 . GLU 83 83 50720 2 . THR 84 84 50720 2 . ILE 85 85 50720 2 . THR 86 86 50720 2 . ALA 87 87 50720 2 . PHE 88 88 50720 2 . VAL 89 89 50720 2 . GLU 90 90 50720 2 . PRO 91 91 50720 2 . PHE 92 92 50720 2 . VAL 93 93 50720 2 . ILE 94 94 50720 2 . LEU 95 95 50720 2 . LEU 96 96 50720 2 . ILE 97 97 50720 2 . LEU 98 98 50720 2 . ILE 99 99 50720 2 . ALA 100 100 50720 2 . ASN 101 101 50720 2 . ALA 102 102 50720 2 . ILE 103 103 50720 2 . VAL 104 104 50720 2 . GLY 105 105 50720 2 . VAL 106 106 50720 2 . TRP 107 107 50720 2 . GLN 108 108 50720 2 . GLU 109 109 50720 2 . ARG 110 110 50720 2 . ASN 111 111 50720 2 . ALA 112 112 50720 2 . GLU 113 113 50720 2 . ASN 114 114 50720 2 . ALA 115 115 50720 2 . ILE 116 116 50720 2 . GLU 117 117 50720 2 . ALA 118 118 50720 2 . LEU 119 119 50720 2 . LYS 120 120 50720 2 . GLU 121 121 50720 2 . TYR 122 122 50720 2 . GLU 123 123 50720 2 . PRO 124 124 50720 2 . GLU 125 125 50720 2 . MET 126 126 50720 2 . GLY 127 127 50720 2 . LYS 128 128 50720 2 . VAL 129 129 50720 2 . TYR 130 130 50720 2 . ARG 131 131 50720 2 . ALA 132 132 50720 2 . ASP 133 133 50720 2 . ARG 134 134 50720 2 . LYS 135 135 50720 2 . SER 136 136 50720 2 . VAL 137 137 50720 2 . GLN 138 138 50720 2 . ARG 139 139 50720 2 . ILE 140 140 50720 2 . LYS 141 141 50720 2 . ALA 142 142 50720 2 . ARG 143 143 50720 2 . ASP 144 144 50720 2 . ILE 145 145 50720 2 . VAL 146 146 50720 2 . PRO 147 147 50720 2 . GLY 148 148 50720 2 . ASP 149 149 50720 2 . ILE 150 150 50720 2 . VAL 151 151 50720 2 . GLU 152 152 50720 2 . VAL 153 153 50720 2 . ALA 154 154 50720 2 . VAL 155 155 50720 2 . GLY 156 156 50720 2 . ASP 157 157 50720 2 . LYS 158 158 50720 2 . VAL 159 159 50720 2 . PRO 160 160 50720 2 . ALA 161 161 50720 2 . ASP 162 162 50720 2 . ILE 163 163 50720 2 . ARG 164 164 50720 2 . ILE 165 165 50720 2 . LEU 166 166 50720 2 . SER 167 167 50720 2 . ILE 168 168 50720 2 . LYS 169 169 50720 2 . SER 170 170 50720 2 . THR 171 171 50720 2 . THR 172 172 50720 2 . LEU 173 173 50720 2 . ARG 174 174 50720 2 . VAL 175 175 50720 2 . ASP 176 176 50720 2 . GLN 177 177 50720 2 . SER 178 178 50720 2 . ILE 179 179 50720 2 . LEU 180 180 50720 2 . THR 181 181 50720 2 . GLY 182 182 50720 2 . GLU 183 183 50720 2 . SER 184 184 50720 2 . VAL 185 185 50720 2 . SER 186 186 50720 2 . VAL 187 187 50720 2 . ILE 188 188 50720 2 . LYS 189 189 50720 2 . HIS 190 190 50720 2 . THR 191 191 50720 2 . GLU 192 192 50720 2 . PRO 193 193 50720 2 . VAL 194 194 50720 2 . PRO 195 195 50720 2 . ASP 196 196 50720 2 . PRO 197 197 50720 2 . ARG 198 198 50720 2 . ALA 199 199 50720 2 . VAL 200 200 50720 2 . ASN 201 201 50720 2 . GLN 202 202 50720 2 . ASP 203 203 50720 2 . LYS 204 204 50720 2 . LYS 205 205 50720 2 . ASN 206 206 50720 2 . MET 207 207 50720 2 . LEU 208 208 50720 2 . PHE 209 209 50720 2 . SER 210 210 50720 2 . GLY 211 211 50720 2 . THR 212 212 50720 2 . ASN 213 213 50720 2 . ILE 214 214 50720 2 . ALA 215 215 50720 2 . ALA 216 216 50720 2 . GLY 217 217 50720 2 . LYS 218 218 50720 2 . ALA 219 219 50720 2 . LEU 220 220 50720 2 . GLY 221 221 50720 2 . ILE 222 222 50720 2 . VAL 223 223 50720 2 . ALA 224 224 50720 2 . THR 225 225 50720 2 . THR 226 226 50720 2 . GLY 227 227 50720 2 . VAL 228 228 50720 2 . SER 229 229 50720 2 . THR 230 230 50720 2 . GLU 231 231 50720 2 . ILE 232 232 50720 2 . GLY 233 233 50720 2 . LYS 234 234 50720 2 . ILE 235 235 50720 2 . ARG 236 236 50720 2 . ASP 237 237 50720 2 . GLN 238 238 50720 2 . MET 239 239 50720 2 . ALA 240 240 50720 2 . ALA 241 241 50720 2 . THR 242 242 50720 2 . GLU 243 243 50720 2 . GLN 244 244 50720 2 . ASP 245 245 50720 2 . LYS 246 246 50720 2 . THR 247 247 50720 2 . PRO 248 248 50720 2 . LEU 249 249 50720 2 . GLN 250 250 50720 2 . GLN 251 251 50720 2 . LYS 252 252 50720 2 . LEU 253 253 50720 2 . ASP 254 254 50720 2 . GLU 255 255 50720 2 . PHE 256 256 50720 2 . GLY 257 257 50720 2 . GLU 258 258 50720 2 . GLN 259 259 50720 2 . LEU 260 260 50720 2 . SER 261 261 50720 2 . LYS 262 262 50720 2 . VAL 263 263 50720 2 . ILE 264 264 50720 2 . SER 265 265 50720 2 . LEU 266 266 50720 2 . ILE 267 267 50720 2 . CYS 268 268 50720 2 . VAL 269 269 50720 2 . ALA 270 270 50720 2 . VAL 271 271 50720 2 . TRP 272 272 50720 2 . LEU 273 273 50720 2 . ILE 274 274 50720 2 . ASN 275 275 50720 2 . ILE 276 276 50720 2 . GLY 277 277 50720 2 . HIS 278 278 50720 2 . PHE 279 279 50720 2 . ASN 280 280 50720 2 . ASP 281 281 50720 2 . PRO 282 282 50720 2 . VAL 283 283 50720 2 . HIS 284 284 50720 2 . GLY 285 285 50720 2 . GLY 286 286 50720 2 . SER 287 287 50720 2 . TRP 288 288 50720 2 . ILE 289 289 50720 2 . ARG 290 290 50720 2 . GLY 291 291 50720 2 . ALA 292 292 50720 2 . ILE 293 293 50720 2 . TYR 294 294 50720 2 . TYR 295 295 50720 2 . PHE 296 296 50720 2 . LYS 297 297 50720 2 . ILE 298 298 50720 2 . ALA 299 299 50720 2 . VAL 300 300 50720 2 . ALA 301 301 50720 2 . LEU 302 302 50720 2 . ALA 303 303 50720 2 . VAL 304 304 50720 2 . ALA 305 305 50720 2 . ALA 306 306 50720 2 . ILE 307 307 50720 2 . PRO 308 308 50720 2 . GLU 309 309 50720 2 . GLY 310 310 50720 2 . LEU 311 311 50720 2 . PRO 312 312 50720 2 . ALA 313 313 50720 2 . VAL 314 314 50720 2 . ILE 315 315 50720 2 . THR 316 316 50720 2 . THR 317 317 50720 2 . CYS 318 318 50720 2 . LEU 319 319 50720 2 . ALA 320 320 50720 2 . LEU 321 321 50720 2 . GLY 322 322 50720 2 . THR 323 323 50720 2 . ARG 324 324 50720 2 . ARG 325 325 50720 2 . MET 326 326 50720 2 . ALA 327 327 50720 2 . LYS 328 328 50720 2 . LYS 329 329 50720 2 . ASN 330 330 50720 2 . ALA 331 331 50720 2 . ILE 332 332 50720 2 . VAL 333 333 50720 2 . ARG 334 334 50720 2 . SER 335 335 50720 2 . LEU 336 336 50720 2 . PRO 337 337 50720 2 . SER 338 338 50720 2 . VAL 339 339 50720 2 . GLU 340 340 50720 2 . THR 341 341 50720 2 . LEU 342 342 50720 2 . GLY 343 343 50720 2 . CYS 344 344 50720 2 . THR 345 345 50720 2 . SER 346 346 50720 2 . VAL 347 347 50720 2 . ILE 348 348 50720 2 . CYS 349 349 50720 2 . SER 350 350 50720 2 . ASP 351 351 50720 2 . LYS 352 352 50720 2 . THR 353 353 50720 2 . GLY 354 354 50720 2 . THR 355 355 50720 2 . LEU 356 356 50720 2 . THR 357 357 50720 2 . THR 358 358 50720 2 . ASN 359 359 50720 2 . GLN 360 360 50720 2 . MET 361 361 50720 2 . SER 362 362 50720 2 . VAL 363 363 50720 2 . CYS 364 364 50720 2 . LYS 365 365 50720 2 . MET 366 366 50720 2 . PHE 367 367 50720 2 . ILE 368 368 50720 2 . ILE 369 369 50720 2 . ASP 370 370 50720 2 . LYS 371 371 50720 2 . VAL 372 372 50720 2 . ASP 373 373 50720 2 . GLY 374 374 50720 2 . ASP 375 375 50720 2 . PHE 376 376 50720 2 . CYS 377 377 50720 2 . SER 378 378 50720 2 . LEU 379 379 50720 2 . ASN 380 380 50720 2 . GLU 381 381 50720 2 . PHE 382 382 50720 2 . SER 383 383 50720 2 . ILE 384 384 50720 2 . THR 385 385 50720 2 . GLY 386 386 50720 2 . SER 387 387 50720 2 . THR 388 388 50720 2 . TYR 389 389 50720 2 . ALA 390 390 50720 2 . PRO 391 391 50720 2 . GLU 392 392 50720 2 . GLY 393 393 50720 2 . GLU 394 394 50720 2 . VAL 395 395 50720 2 . LEU 396 396 50720 2 . LYS 397 397 50720 2 . ASN 398 398 50720 2 . ASP 399 399 50720 2 . LYS 400 400 50720 2 . PRO 401 401 50720 2 . ILE 402 402 50720 2 . ARG 403 403 50720 2 . SER 404 404 50720 2 . GLY 405 405 50720 2 . GLN 406 406 50720 2 . PHE 407 407 50720 2 . ASP 408 408 50720 2 . GLY 409 409 50720 2 . LEU 410 410 50720 2 . VAL 411 411 50720 2 . GLU 412 412 50720 2 . LEU 413 413 50720 2 . ALA 414 414 50720 2 . THR 415 415 50720 2 . ILE 416 416 50720 2 . CYS 417 417 50720 2 . ALA 418 418 50720 2 . LEU 419 419 50720 2 . CYS 420 420 50720 2 . ASN 421 421 50720 2 . ASP 422 422 50720 2 . SER 423 423 50720 2 . SER 424 424 50720 2 . LEU 425 425 50720 2 . ASP 426 426 50720 2 . PHE 427 427 50720 2 . ASN 428 428 50720 2 . GLU 429 429 50720 2 . THR 430 430 50720 2 . LYS 431 431 50720 2 . GLY 432 432 50720 2 . VAL 433 433 50720 2 . TYR 434 434 50720 2 . GLU 435 435 50720 2 . LYS 436 436 50720 2 . VAL 437 437 50720 2 . GLY 438 438 50720 2 . GLU 439 439 50720 2 . ALA 440 440 50720 2 . THR 441 441 50720 2 . GLU 442 442 50720 2 . THR 443 443 50720 2 . ALA 444 444 50720 2 . LEU 445 445 50720 2 . THR 446 446 50720 2 . THR 447 447 50720 2 . LEU 448 448 50720 2 . VAL 449 449 50720 2 . GLU 450 450 50720 2 . LYS 451 451 50720 2 . MET 452 452 50720 2 . ASN 453 453 50720 2 . VAL 454 454 50720 2 . PHE 455 455 50720 2 . ASN 456 456 50720 2 . THR 457 457 50720 2 . GLU 458 458 50720 2 . VAL 459 459 50720 2 . ARG 460 460 50720 2 . ASN 461 461 50720 2 . LEU 462 462 50720 2 . SER 463 463 50720 2 . LYS 464 464 50720 2 . VAL 465 465 50720 2 . GLU 466 466 50720 2 . ARG 467 467 50720 2 . ALA 468 468 50720 2 . ASN 469 469 50720 2 . ALA 470 470 50720 2 . CYS 471 471 50720 2 . ASN 472 472 50720 2 . SER 473 473 50720 2 . VAL 474 474 50720 2 . ILE 475 475 50720 2 . ARG 476 476 50720 2 . GLN 477 477 50720 2 . LEU 478 478 50720 2 . MET 479 479 50720 2 . LYS 480 480 50720 2 . LYS 481 481 50720 2 . GLU 482 482 50720 2 . PHE 483 483 50720 2 . THR 484 484 50720 2 . LEU 485 485 50720 2 . GLU 486 486 50720 2 . PHE 487 487 50720 2 . SER 488 488 50720 2 . ARG 489 489 50720 2 . ASP 490 490 50720 2 . ARG 491 491 50720 2 . LYS 492 492 50720 2 . SER 493 493 50720 2 . MET 494 494 50720 2 . SER 495 495 50720 2 . VAL 496 496 50720 2 . TYR 497 497 50720 2 . CYS 498 498 50720 2 . SER 499 499 50720 2 . PRO 500 500 50720 2 . ALA 501 501 50720 2 . LYS 502 502 50720 2 . SER 503 503 50720 2 . SER 504 504 50720 2 . ARG 505 505 50720 2 . ALA 506 506 50720 2 . ALA 507 507 50720 2 . VAL 508 508 50720 2 . GLY 509 509 50720 2 . ASN 510 510 50720 2 . LYS 511 511 50720 2 . MET 512 512 50720 2 . PHE 513 513 50720 2 . VAL 514 514 50720 2 . LYS 515 515 50720 2 . GLY 516 516 50720 2 . ALA 517 517 50720 2 . PRO 518 518 50720 2 . GLU 519 519 50720 2 . GLY 520 520 50720 2 . VAL 521 521 50720 2 . ILE 522 522 50720 2 . ASP 523 523 50720 2 . ARG 524 524 50720 2 . CYS 525 525 50720 2 . ASN 526 526 50720 2 . TYR 527 527 50720 2 . VAL 528 528 50720 2 . ARG 529 529 50720 2 . VAL 530 530 50720 2 . GLY 531 531 50720 2 . THR 532 532 50720 2 . THR 533 533 50720 2 . ARG 534 534 50720 2 . VAL 535 535 50720 2 . PRO 536 536 50720 2 . MET 537 537 50720 2 . THR 538 538 50720 2 . GLY 539 539 50720 2 . PRO 540 540 50720 2 . VAL 541 541 50720 2 . LYS 542 542 50720 2 . GLU 543 543 50720 2 . LYS 544 544 50720 2 . ILE 545 545 50720 2 . LEU 546 546 50720 2 . SER 547 547 50720 2 . VAL 548 548 50720 2 . ILE 549 549 50720 2 . LYS 550 550 50720 2 . GLU 551 551 50720 2 . TRP 552 552 50720 2 . GLY 553 553 50720 2 . THR 554 554 50720 2 . GLY 555 555 50720 2 . ARG 556 556 50720 2 . ASP 557 557 50720 2 . THR 558 558 50720 2 . LEU 559 559 50720 2 . ARG 560 560 50720 2 . CYS 561 561 50720 2 . LEU 562 562 50720 2 . ALA 563 563 50720 2 . LEU 564 564 50720 2 . ALA 565 565 50720 2 . THR 566 566 50720 2 . ARG 567 567 50720 2 . ASP 568 568 50720 2 . THR 569 569 50720 2 . PRO 570 570 50720 2 . PRO 571 571 50720 2 . LYS 572 572 50720 2 . ARG 573 573 50720 2 . GLU 574 574 50720 2 . GLU 575 575 50720 2 . MET 576 576 50720 2 . VAL 577 577 50720 2 . LEU 578 578 50720 2 . ASP 579 579 50720 2 . ASP 580 580 50720 2 . SER 581 581 50720 2 . SER 582 582 50720 2 . ARG 583 583 50720 2 . PHE 584 584 50720 2 . MET 585 585 50720 2 . GLU 586 586 50720 2 . TYR 587 587 50720 2 . GLU 588 588 50720 2 . THR 589 589 50720 2 . ASP 590 590 50720 2 . LEU 591 591 50720 2 . THR 592 592 50720 2 . PHE 593 593 50720 2 . VAL 594 594 50720 2 . GLY 595 595 50720 2 . VAL 596 596 50720 2 . VAL 597 597 50720 2 . GLY 598 598 50720 2 . MET 599 599 50720 2 . LEU 600 600 50720 2 . ASP 601 601 50720 2 . PRO 602 602 50720 2 . PRO 603 603 50720 2 . ARG 604 604 50720 2 . LYS 605 605 50720 2 . GLU 606 606 50720 2 . VAL 607 607 50720 2 . MET 608 608 50720 2 . GLY 609 609 50720 2 . SER 610 610 50720 2 . ILE 611 611 50720 2 . GLN 612 612 50720 2 . LEU 613 613 50720 2 . CYS 614 614 50720 2 . ARG 615 615 50720 2 . ASP 616 616 50720 2 . ALA 617 617 50720 2 . GLY 618 618 50720 2 . ILE 619 619 50720 2 . ARG 620 620 50720 2 . VAL 621 621 50720 2 . ILE 622 622 50720 2 . MET 623 623 50720 2 . ILE 624 624 50720 2 . THR 625 625 50720 2 . GLY 626 626 50720 2 . ASP 627 627 50720 2 . ASN 628 628 50720 2 . LYS 629 629 50720 2 . GLY 630 630 50720 2 . THR 631 631 50720 2 . ALA 632 632 50720 2 . ILE 633 633 50720 2 . ALA 634 634 50720 2 . ILE 635 635 50720 2 . CYS 636 636 50720 2 . ARG 637 637 50720 2 . ARG 638 638 50720 2 . ILE 639 639 50720 2 . GLY 640 640 50720 2 . ILE 641 641 50720 2 . PHE 642 642 50720 2 . GLY 643 643 50720 2 . GLU 644 644 50720 2 . ASN 645 645 50720 2 . GLU 646 646 50720 2 . GLU 647 647 50720 2 . VAL 648 648 50720 2 . ALA 649 649 50720 2 . ASP 650 650 50720 2 . ARG 651 651 50720 2 . ALA 652 652 50720 2 . TYR 653 653 50720 2 . THR 654 654 50720 2 . GLY 655 655 50720 2 . ARG 656 656 50720 2 . GLU 657 657 50720 2 . PHE 658 658 50720 2 . ASP 659 659 50720 2 . ASP 660 660 50720 2 . LEU 661 661 50720 2 . PRO 662 662 50720 2 . LEU 663 663 50720 2 . ALA 664 664 50720 2 . GLU 665 665 50720 2 . GLN 666 666 50720 2 . ARG 667 667 50720 2 . GLU 668 668 50720 2 . ALA 669 669 50720 2 . CYS 670 670 50720 2 . ARG 671 671 50720 2 . ARG 672 672 50720 2 . ALA 673 673 50720 2 . CYS 674 674 50720 2 . CYS 675 675 50720 2 . PHE 676 676 50720 2 . ALA 677 677 50720 2 . ARG 678 678 50720 2 . VAL 679 679 50720 2 . GLU 680 680 50720 2 . PRO 681 681 50720 2 . SER 682 682 50720 2 . HIS 683 683 50720 2 . LYS 684 684 50720 2 . SER 685 685 50720 2 . LYS 686 686 50720 2 . ILE 687 687 50720 2 . VAL 688 688 50720 2 . GLU 689 689 50720 2 . TYR 690 690 50720 2 . LEU 691 691 50720 2 . GLN 692 692 50720 2 . SER 693 693 50720 2 . TYR 694 694 50720 2 . ASP 695 695 50720 2 . GLU 696 696 50720 2 . ILE 697 697 50720 2 . THR 698 698 50720 2 . ALA 699 699 50720 2 . MET 700 700 50720 2 . THR 701 701 50720 2 . GLY 702 702 50720 2 . ASP 703 703 50720 2 . GLY 704 704 50720 2 . VAL 705 705 50720 2 . ASN 706 706 50720 2 . ASP 707 707 50720 2 . ALA 708 708 50720 2 . PRO 709 709 50720 2 . ALA 710 710 50720 2 . LEU 711 711 50720 2 . LYS 712 712 50720 2 . LYS 713 713 50720 2 . ALA 714 714 50720 2 . GLU 715 715 50720 2 . ILE 716 716 50720 2 . GLY 717 717 50720 2 . ILE 718 718 50720 2 . ALA 719 719 50720 2 . MET 720 720 50720 2 . GLY 721 721 50720 2 . SER 722 722 50720 2 . GLY 723 723 50720 2 . THR 724 724 50720 2 . ALA 725 725 50720 2 . VAL 726 726 50720 2 . ALA 727 727 50720 2 . LYS 728 728 50720 2 . THR 729 729 50720 2 . ALA 730 730 50720 2 . SER 731 731 50720 2 . GLU 732 732 50720 2 . MET 733 733 50720 2 . VAL 734 734 50720 2 . LEU 735 735 50720 2 . ALA 736 736 50720 2 . ASP 737 737 50720 2 . ASP 738 738 50720 2 . ASN 739 739 50720 2 . PHE 740 740 50720 2 . SER 741 741 50720 2 . THR 742 742 50720 2 . ILE 743 743 50720 2 . VAL 744 744 50720 2 . ALA 745 745 50720 2 . ALA 746 746 50720 2 . VAL 747 747 50720 2 . GLU 748 748 50720 2 . GLU 749 749 50720 2 . GLY 750 750 50720 2 . ARG 751 751 50720 2 . ALA 752 752 50720 2 . ILE 753 753 50720 2 . TYR 754 754 50720 2 . ASN 755 755 50720 2 . ASN 756 756 50720 2 . MET 757 757 50720 2 . LYS 758 758 50720 2 . GLN 759 759 50720 2 . PHE 760 760 50720 2 . ILE 761 761 50720 2 . ARG 762 762 50720 2 . TYR 763 763 50720 2 . LEU 764 764 50720 2 . ILE 765 765 50720 2 . SER 766 766 50720 2 . SER 767 767 50720 2 . ASN 768 768 50720 2 . VAL 769 769 50720 2 . GLY 770 770 50720 2 . GLU 771 771 50720 2 . VAL 772 772 50720 2 . VAL 773 773 50720 2 . CYS 774 774 50720 2 . ILE 775 775 50720 2 . PHE 776 776 50720 2 . LEU 777 777 50720 2 . THR 778 778 50720 2 . ALA 779 779 50720 2 . ALA 780 780 50720 2 . LEU 781 781 50720 2 . GLY 782 782 50720 2 . LEU 783 783 50720 2 . PRO 784 784 50720 2 . GLU 785 785 50720 2 . ALA 786 786 50720 2 . LEU 787 787 50720 2 . ILE 788 788 50720 2 . PRO 789 789 50720 2 . VAL 790 790 50720 2 . GLN 791 791 50720 2 . LEU 792 792 50720 2 . LEU 793 793 50720 2 . TRP 794 794 50720 2 . VAL 795 795 50720 2 . ASN 796 796 50720 2 . LEU 797 797 50720 2 . VAL 798 798 50720 2 . THR 799 799 50720 2 . ASP 800 800 50720 2 . GLY 801 801 50720 2 . LEU 802 802 50720 2 . PRO 803 803 50720 2 . ALA 804 804 50720 2 . THR 805 805 50720 2 . ALA 806 806 50720 2 . LEU 807 807 50720 2 . GLY 808 808 50720 2 . PHE 809 809 50720 2 . ASN 810 810 50720 2 . PRO 811 811 50720 2 . PRO 812 812 50720 2 . ASP 813 813 50720 2 . LEU 814 814 50720 2 . ASP 815 815 50720 2 . ILE 816 816 50720 2 . MET 817 817 50720 2 . ASP 818 818 50720 2 . ARG 819 819 50720 2 . PRO 820 820 50720 2 . PRO 821 821 50720 2 . ARG 822 822 50720 2 . SER 823 823 50720 2 . PRO 824 824 50720 2 . LYS 825 825 50720 2 . GLU 826 826 50720 2 . PRO 827 827 50720 2 . LEU 828 828 50720 2 . ILE 829 829 50720 2 . SER 830 830 50720 2 . GLY 831 831 50720 2 . TRP 832 832 50720 2 . LEU 833 833 50720 2 . PHE 834 834 50720 2 . PHE 835 835 50720 2 . ARG 836 836 50720 2 . TYR 837 837 50720 2 . MET 838 838 50720 2 . ALA 839 839 50720 2 . ILE 840 840 50720 2 . GLY 841 841 50720 2 . GLY 842 842 50720 2 . TYR 843 843 50720 2 . VAL 844 844 50720 2 . GLY 845 845 50720 2 . ALA 846 846 50720 2 . ALA 847 847 50720 2 . THR 848 848 50720 2 . VAL 849 849 50720 2 . GLY 850 850 50720 2 . ALA 851 851 50720 2 . ALA 852 852 50720 2 . ALA 853 853 50720 2 . TRP 854 854 50720 2 . TRP 855 855 50720 2 . PHE 856 856 50720 2 . MET 857 857 50720 2 . TYR 858 858 50720 2 . ALA 859 859 50720 2 . GLU 860 860 50720 2 . ASP 861 861 50720 2 . GLY 862 862 50720 2 . PRO 863 863 50720 2 . GLY 864 864 50720 2 . VAL 865 865 50720 2 . THR 866 866 50720 2 . TYR 867 867 50720 2 . HIS 868 868 50720 2 . GLN 869 869 50720 2 . LEU 870 870 50720 2 . THR 871 871 50720 2 . HIS 872 872 50720 2 . PHE 873 873 50720 2 . MET 874 874 50720 2 . GLN 875 875 50720 2 . CYS 876 876 50720 2 . THR 877 877 50720 2 . GLU 878 878 50720 2 . ASP 879 879 50720 2 . HIS 880 880 50720 2 . PRO 881 881 50720 2 . HIS 882 882 50720 2 . PHE 883 883 50720 2 . GLU 884 884 50720 2 . GLY 885 885 50720 2 . LEU 886 886 50720 2 . ASP 887 887 50720 2 . CYS 888 888 50720 2 . GLU 889 889 50720 2 . ILE 890 890 50720 2 . PHE 891 891 50720 2 . GLU 892 892 50720 2 . ALA 893 893 50720 2 . PRO 894 894 50720 2 . GLU 895 895 50720 2 . PRO 896 896 50720 2 . MET 897 897 50720 2 . THR 898 898 50720 2 . MET 899 899 50720 2 . ALA 900 900 50720 2 . LEU 901 901 50720 2 . SER 902 902 50720 2 . VAL 903 903 50720 2 . LEU 904 904 50720 2 . VAL 905 905 50720 2 . THR 906 906 50720 2 . ILE 907 907 50720 2 . GLU 908 908 50720 2 . MET 909 909 50720 2 . CYS 910 910 50720 2 . ASN 911 911 50720 2 . ALA 912 912 50720 2 . LEU 913 913 50720 2 . ASN 914 914 50720 2 . SER 915 915 50720 2 . LEU 916 916 50720 2 . SER 917 917 50720 2 . GLU 918 918 50720 2 . ASN 919 919 50720 2 . GLN 920 920 50720 2 . SER 921 921 50720 2 . LEU 922 922 50720 2 . MET 923 923 50720 2 . ARG 924 924 50720 2 . MET 925 925 50720 2 . PRO 926 926 50720 2 . PRO 927 927 50720 2 . TRP 928 928 50720 2 . VAL 929 929 50720 2 . ASN 930 930 50720 2 . ILE 931 931 50720 2 . TRP 932 932 50720 2 . LEU 933 933 50720 2 . LEU 934 934 50720 2 . GLY 935 935 50720 2 . SER 936 936 50720 2 . ILE 937 937 50720 2 . CYS 938 938 50720 2 . LEU 939 939 50720 2 . SER 940 940 50720 2 . MET 941 941 50720 2 . SER 942 942 50720 2 . LEU 943 943 50720 2 . HIS 944 944 50720 2 . PHE 945 945 50720 2 . LEU 946 946 50720 2 . ILE 947 947 50720 2 . LEU 948 948 50720 2 . TYR 949 949 50720 2 . VAL 950 950 50720 2 . ASP 951 951 50720 2 . PRO 952 952 50720 2 . LEU 953 953 50720 2 . PRO 954 954 50720 2 . MET 955 955 50720 2 . ILE 956 956 50720 2 . PHE 957 957 50720 2 . LYS 958 958 50720 2 . LEU 959 959 50720 2 . LYS 960 960 50720 2 . ALA 961 961 50720 2 . LEU 962 962 50720 2 . ASP 963 963 50720 2 . LEU 964 964 50720 2 . THR 965 965 50720 2 . GLN 966 966 50720 2 . TRP 967 967 50720 2 . LEU 968 968 50720 2 . MET 969 969 50720 2 . VAL 970 970 50720 2 . LEU 971 971 50720 2 . LYS 972 972 50720 2 . ILE 973 973 50720 2 . SER 974 974 50720 2 . LEU 975 975 50720 2 . PRO 976 976 50720 2 . VAL 977 977 50720 2 . ILE 978 978 50720 2 . GLY 979 979 50720 2 . LEU 980 980 50720 2 . ASP 981 981 50720 2 . GLU 982 982 50720 2 . ILE 983 983 50720 2 . LEU 984 984 50720 2 . LYS 985 985 50720 2 . PHE 986 986 50720 2 . ILE 987 987 50720 2 . ALA 988 988 50720 2 . ARG 989 989 50720 2 . ASN 990 990 50720 2 . TYR 991 991 50720 2 . LEU 992 992 50720 2 . GLU 993 993 50720 2 . ASP 994 994 50720 2 . PRO 995 995 50720 2 . GLU 996 996 50720 2 . ASP 997 997 50720 2 . GLU 998 998 50720 2 . ARG 999 999 50720 2 . ARG 1000 1000 50720 2 . LYS 1001 1001 50720 2 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 50720 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9986 organism . 'Oryctolagus cuniculus' rabbit . . Eukaryota Metazoa Oryctolagus cuniculus . . . . . . . . . . . . . 50720 1 2 2 $entity_2 . 9986 organism . 'Oryctolagus cuniculus' rabbit . . Eukaryota Metazoa Oryctolagus cuniculus . . . . . . . . . . . . . 50720 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 50720 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pMal-c2x . . . 50720 1 2 2 $entity_2 . 'purified from the natural source' . . . . . . . . . plasmid . . . . . . 50720 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 50720 _Sample.ID 1 _Sample.Name 'Monomeric phospholamban in complex with SERCA in oriented bicelles (calcium-free E2 state)' _Sample.Type bicelle _Sample.Sub_type . _Sample.Details 'Flipped blicelles (q = 4)' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system H2O _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Phospholamban (PLN-AFA)' [U-15N] . . 1 $entity_1 . . 0.21 . . mM . . . . 50720 1 2 HEPES 'natural abundance' . . . . . . 20 . . mM . . . . 50720 1 3 'potassium chloride' 'natural abundance' . . . . . . 100 . . mM . . . . 50720 1 4 'sodium azide' 'natural abundance' . . . . . . 1 . . mM . . . . 50720 1 5 glycerol 'natural abundance' . . . . . . 2.5 . . % . . . . 50720 1 6 '1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)' 'natural abundance' . . . . . . 340 . . mM . . . . 50720 1 7 '1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC)' 'natural abundance' . . . . . . 85 . . mM . . . . 50720 1 8 '1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC)' 'natural abundance' . . . . . . 106 . . mM . . . . 50720 1 9 '1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (PE-DTPA)' 'natural abundance' . . . . . . 6.4 . . mM . . . . 50720 1 10 'Ytterbium(III) chloride' 'natural abundance' . . . . . . 5 . . mM . . . . 50720 1 11 'Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (SERCA1a)' 'natural abundance' . . 2 $entity_2 . . 0.23 . . mM . . . . 50720 1 12 DTT 'natural abundance' . . . . . . 2 . . mM . . . . 50720 1 13 'magnesium chloride' 'natural abundance' . . . . . . 5 . . mM . . . . 50720 1 14 "ethylene glycol-bis(b-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA)" 'natural abundance' . . . . . . 4 . . mM . . . . 50720 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 50720 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Phospholamban in complex with SERCA' _Sample_condition_list.Details 'Monomeric phospholamban reconstituted with SERCA in bicelles.' loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 7 . pH 50720 1 pressure 1 . atm 50720 1 temperature 298.15 . K 50720 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 50720 _Software.ID 1 _Software.Type . _Software.Name NMRPipe _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 50720 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 50720 _Software.ID 2 _Software.Type . _Software.Name VNMRj _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 50720 2 stop_ save_ save_software_3 _Software.Sf_category software _Software.Sf_framecode software_3 _Software.Entry_ID 50720 _Software.ID 3 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'data analysis' . 50720 3 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 50720 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 'Varian VNMRS - 700 MHz' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model VXRS _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 700 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 50720 _Experiment_list.ID 1 _Experiment_list.Details 'Orientated 15N chemical shift correlations with 15N-1H dipolar couplings.' loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D NH SLF' yes . . . . . . . . . . . . 1 $sample_1 anisotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50720 1 stop_ loop_ _Experiment_file.Experiment_ID _Experiment_file.Experiment_name _Experiment_file.Name _Experiment_file.Type _Experiment_file.Content _Experiment_file.Directory_path _Experiment_file.Details _Experiment_file.Entry_ID _Experiment_file.Experiment_list_ID 1 '2D NH SLF' sample3_afa-serca-e2_rawdata.zip . 'NMR experiment directory' . . 50720 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 50720 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 15N _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID N 15 '[15N] ammonium chloride' nitrogen . . . . ppm 39.3 external direct 1 . . . . . 50720 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 50720 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'PLN-AFA with SERCA (E2) 15N Shifts' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details 'Anisotropic chemical shifts and dipolar couplings (Hz) measured using a PISEMA-like experiment (SE-SAMPI4).' _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D NH SLF' . . . 50720 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 3 $software_3 . . 50720 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 37 37 ALA N N 15 187.054 5 . . . . . . . 36 ALA N . 50720 1 2 . 1 . 1 38 38 LEU N N 15 137.132 5 . . . . . . . 37 LEU N . 50720 1 3 . 1 . 1 39 39 ILE N N 15 157.634 5 . . . . . . . 38 ILE N . 50720 1 4 . 1 . 1 40 40 LEU N N 15 190.889 5 . . . . . . . 39 LEU N . 50720 1 5 . 1 . 1 41 41 ILE N N 15 164.627 5 . . . . . . . 40 ILE N . 50720 1 6 . 1 . 1 43 43 LEU N N 15 176.074 5 . . . . . . . 42 LEU N . 50720 1 7 . 1 . 1 44 44 LEU N N 15 193.352 5 . . . . . . . 43 LEU N . 50720 1 8 . 1 . 1 45 45 LEU N N 15 150.525 5 . . . . . . . 44 LEU N . 50720 1 9 . 1 . 1 46 46 ILE N N 15 153.316 5 . . . . . . . 45 ILE N . 50720 1 10 . 1 . 1 49 49 ILE N N 15 146.076 5 . . . . . . . 48 ILE N . 50720 1 stop_ save_ ################################ # Dipolar coupling constants # ################################ save_dipolar_couplings_1 _Dipolar_coupling_list.Sf_category dipolar_couplings _Dipolar_coupling_list.Sf_framecode dipolar_couplings_1 _Dipolar_coupling_list.Entry_ID 50720 _Dipolar_coupling_list.ID 1 _Dipolar_coupling_list.Name 'PLN-AFA with SERCA (E2) 15N-1H Dipolar Couplings' _Dipolar_coupling_list.Sample_condition_list_ID 1 _Dipolar_coupling_list.Sample_condition_list_label $sample_conditions_1 _Dipolar_coupling_list.Spectrometer_frequency_1H 700 _Dipolar_coupling_list.Scaling_factor . _Dipolar_coupling_list.Fitting_procedure . _Dipolar_coupling_list.Details 'Anisotropic chemical shifts and dipolar couplings (Hz) measured using a PISEMA-like experiment (SE-SAMPI4).' _Dipolar_coupling_list.Text_data_format . _Dipolar_coupling_list.Text_data . loop_ _Dipolar_coupling_experiment.Experiment_ID _Dipolar_coupling_experiment.Experiment_name _Dipolar_coupling_experiment.Sample_ID _Dipolar_coupling_experiment.Sample_label _Dipolar_coupling_experiment.Sample_state _Dipolar_coupling_experiment.Entry_ID _Dipolar_coupling_experiment.Dipolar_coupling_list_ID 1 '2D NH SLF' . . . 50720 1 stop_ loop_ _Dipolar_coupling_software.Software_ID _Dipolar_coupling_software.Software_label _Dipolar_coupling_software.Method_ID _Dipolar_coupling_software.Method_label _Dipolar_coupling_software.Entry_ID _Dipolar_coupling_software.Dipolar_coupling_list_ID 3 $software_3 . . 50720 1 stop_ loop_ _Dipolar_coupling.ID _Dipolar_coupling.Dipolar_coupling_code _Dipolar_coupling.Assembly_atom_ID_1 _Dipolar_coupling.Entity_assembly_ID_1 _Dipolar_coupling.Entity_ID_1 _Dipolar_coupling.Comp_index_ID_1 _Dipolar_coupling.Seq_ID_1 _Dipolar_coupling.Comp_ID_1 _Dipolar_coupling.Atom_ID_1 _Dipolar_coupling.Atom_type_1 _Dipolar_coupling.Atom_isotope_number_1 _Dipolar_coupling.Ambiguity_code_1 _Dipolar_coupling.Assembly_atom_ID_2 _Dipolar_coupling.Entity_assembly_ID_2 _Dipolar_coupling.Entity_ID_2 _Dipolar_coupling.Comp_index_ID_2 _Dipolar_coupling.Seq_ID_2 _Dipolar_coupling.Comp_ID_2 _Dipolar_coupling.Atom_ID_2 _Dipolar_coupling.Atom_type_2 _Dipolar_coupling.Atom_isotope_number_2 _Dipolar_coupling.Ambiguity_code_2 _Dipolar_coupling.Val _Dipolar_coupling.Val_min _Dipolar_coupling.Val_max _Dipolar_coupling.Val_err _Dipolar_coupling.Principal_Euler_angle_alpha_val _Dipolar_coupling.Principal_Euler_angle_beta_val _Dipolar_coupling.Principal_Euler_angle_gamma_val _Dipolar_coupling.Resonance_ID_1 _Dipolar_coupling.Resonance_ID_2 _Dipolar_coupling.Auth_entity_assembly_ID_1 _Dipolar_coupling.Auth_seq_ID_1 _Dipolar_coupling.Auth_comp_ID_1 _Dipolar_coupling.Auth_atom_ID_1 _Dipolar_coupling.Auth_entity_assembly_ID_2 _Dipolar_coupling.Auth_seq_ID_2 _Dipolar_coupling.Auth_comp_ID_2 _Dipolar_coupling.Auth_atom_ID_2 _Dipolar_coupling.Entry_ID _Dipolar_coupling.Dipolar_coupling_list_ID 1 . . 1 1 37 37 ALA N N 15 . . 1 1 37 37 ALA H H 1 . 5723 . . 500 . . . . . . 36 ALA N . 36 ALA H 50720 1 2 . . 1 1 38 38 LEU N N 15 . . 1 1 38 38 LEU H H 1 . 6661 . . 500 . . . . . . 37 LEU N . 37 LEU H 50720 1 3 . . 1 1 39 39 ILE N N 15 . . 1 1 39 39 ILE H H 1 . 1893 . . 500 . . . . . . 38 ILE N . 38 ILE H 50720 1 4 . . 1 1 40 40 LEU N N 15 . . 1 1 40 40 LEU H H 1 . 3876 . . 500 . . . . . . 39 LEU N . 39 LEU H 50720 1 5 . . 1 1 41 41 ILE N N 15 . . 1 1 41 41 ILE H H 1 . 7043 . . 500 . . . . . . 40 ILE N . 40 ILE H 50720 1 6 . . 1 1 43 43 LEU N N 15 . . 1 1 43 43 LEU H H 1 . 1741 . . 500 . . . . . . 42 LEU N . 42 LEU H 50720 1 7 . . 1 1 44 44 LEU N N 15 . . 1 1 44 44 LEU H H 1 . 5765 . . 500 . . . . . . 43 LEU N . 43 LEU H 50720 1 8 . . 1 1 45 45 LEU N N 15 . . 1 1 45 45 LEU H H 1 . 6216 . . 500 . . . . . . 44 LEU N . 44 LEU H 50720 1 9 . . 1 1 46 46 ILE N N 15 . . 1 1 46 46 ILE H H 1 . 2744 . . 500 . . . . . . 45 ILE N . 45 ILE H 50720 1 10 . . 1 1 49 49 ILE N N 15 . . 1 1 49 49 ILE H H 1 . 5155 . . 500 . . . . . . 48 ILE N . 48 ILE H 50720 1 stop_ save_