data_50214 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1HN and 15N assignment of PhoQ PD at pH 6.5 ; _BMRB_accession_number 50214 _BMRB_flat_file_name bmr50214.str _Entry_type original _Submission_date 2020-03-18 _Accession_date 2020-03-18 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hicks Kevin G. . 2 Scott Delbecq P. . 3 Enea Sancho-Vaello . . 4 Marie-Pierre Blanc . . 5 Katja Dove K. . 6 Lynne Prost R. . 7 Margaret Daley E. . 8 Kornelius Zeth . . 9 Rachel Klevit E. . 10 Samuel Miller I. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 125 "15N chemical shifts" 125 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-04-21 original BMRB . stop_ _Original_release_date 2020-03-20 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Acidic pH and divalent cation sensing by PhoQ are dispensable for systemic salmonellae virulence. ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 26002083 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hicks Kevin G. . 2 Delbecq Scott P. . 3 Sancho-Vaello Enea . . 4 Blanc Marie-Pierre P. . 5 Dove Katja K. . 6 Prost Lynne R. . 7 Daley Margaret E. . 8 Zeth Kornelius . . 9 Klevit Rachel E. . 10 Miller Samuel I. . stop_ _Journal_abbreviation Elife _Journal_name_full eLife _Journal_volume 4 _Journal_issue . _Journal_ISSN 2050-084X _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first e06792 _Page_last e06792 _Year 2015 _Details . save_ ################################## # Molecular system description # ################################## save_assembly_1 _Saveframe_category molecular_system _Mol_system_name PhoQ_PD _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label PhoQ_PD $entity_1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function 'Sensor Histidine Kinase' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 153 _Mol_residue_sequence ; MDKTTFRLLRGESNLFYTLA KWENNKISVELPENLDMQSP TMTLIYDETGKLLWTQRNIP WLIKSIQPEWLKTNGFHEIE TNVDATSTLLSEDHSAQEKL KEVREDDDDAEMTHSVAVNI YPATARMPQLTIVVVDTIPI ELKRSYMHHHHHH ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 44 MET 2 45 ASP 3 46 LYS 4 47 THR 5 48 THR 6 49 PHE 7 50 ARG 8 51 LEU 9 52 LEU 10 53 ARG 11 54 GLY 12 55 GLU 13 56 SER 14 57 ASN 15 58 LEU 16 59 PHE 17 60 TYR 18 61 THR 19 62 LEU 20 63 ALA 21 64 LYS 22 65 TRP 23 66 GLU 24 67 ASN 25 68 ASN 26 69 LYS 27 70 ILE 28 71 SER 29 72 VAL 30 73 GLU 31 74 LEU 32 75 PRO 33 76 GLU 34 77 ASN 35 78 LEU 36 79 ASP 37 80 MET 38 81 GLN 39 82 SER 40 83 PRO 41 84 THR 42 85 MET 43 86 THR 44 87 LEU 45 88 ILE 46 89 TYR 47 90 ASP 48 91 GLU 49 92 THR 50 93 GLY 51 94 LYS 52 95 LEU 53 96 LEU 54 97 TRP 55 98 THR 56 99 GLN 57 100 ARG 58 101 ASN 59 102 ILE 60 103 PRO 61 104 TRP 62 105 LEU 63 106 ILE 64 107 LYS 65 108 SER 66 109 ILE 67 110 GLN 68 111 PRO 69 112 GLU 70 113 TRP 71 114 LEU 72 115 LYS 73 116 THR 74 117 ASN 75 118 GLY 76 119 PHE 77 120 HIS 78 121 GLU 79 122 ILE 80 123 GLU 81 124 THR 82 125 ASN 83 126 VAL 84 127 ASP 85 128 ALA 86 129 THR 87 130 SER 88 131 THR 89 132 LEU 90 133 LEU 91 134 SER 92 135 GLU 93 136 ASP 94 137 HIS 95 138 SER 96 139 ALA 97 140 GLN 98 141 GLU 99 142 LYS 100 143 LEU 101 144 LYS 102 145 GLU 103 146 VAL 104 147 ARG 105 148 GLU 106 149 ASP 107 150 ASP 108 151 ASP 109 152 ASP 110 153 ALA 111 154 GLU 112 155 MET 113 156 THR 114 157 HIS 115 158 SER 116 159 VAL 117 160 ALA 118 161 VAL 119 162 ASN 120 163 ILE 121 164 TYR 122 165 PRO 123 166 ALA 124 167 THR 125 168 ALA 126 169 ARG 127 170 MET 128 171 PRO 129 172 GLN 130 173 LEU 131 174 THR 132 175 ILE 133 176 VAL 134 177 VAL 135 178 VAL 136 179 ASP 137 180 THR 138 181 ILE 139 182 PRO 140 183 ILE 141 184 GLU 142 185 LEU 143 186 LYS 144 187 ARG 145 188 SER 146 189 TYR 147 190 MET 148 191 HIS 149 192 HIS 150 193 HIS 151 194 HIS 152 195 HIS 153 196 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value UNP D0ZV89.1 'Virulence sensor histidine kinase PhoQ' . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 'Salmonella typhimurium' 28901 Bacteria . Salmonella enterica stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . Escherichia coli . plasmid 'pET11a-phoQ 45-190-(His)6' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 400 uM '[U-100% 15N]' 'sodium phosphate' 20 mM 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' 'magnesium chloride' 20 mM 'natural abundance' EDTA 20 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name 'CcpNmr Analysis' _Version . loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.2 . M pH 6.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.7 internal direct . . . 1 water N 15 protons ppm 4.7 internal indirect . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $software_1 stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name PhoQ_PD _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 45 2 ASP H H 8.435 0.000 1 2 45 2 ASP N N 125.354 0.000 1 3 46 3 LYS H H 8.711 0.000 1 4 46 3 LYS N N 123.344 0.000 1 5 47 4 THR H H 8.037 0.000 1 6 47 4 THR N N 115.111 0.000 1 7 48 5 THR H H 7.874 0.000 1 8 48 5 THR N N 119.837 0.000 1 9 49 6 PHE H H 8.091 0.000 1 10 49 6 PHE N N 121.911 0.000 1 11 50 7 ARG H H 8.071 0.000 1 12 50 7 ARG N N 118.992 0.000 1 13 51 8 LEU H H 8.051 0.000 1 14 51 8 LEU N N 122.617 0.000 1 15 54 11 GLY H H 8.193 0.000 1 16 54 11 GLY N N 107.915 0.000 1 17 55 12 GLU H H 8.098 0.000 1 18 55 12 GLU N N 123.421 0.000 1 19 56 13 SER H H 8.029 0.000 1 20 56 13 SER N N 114.471 0.000 1 21 57 14 ASN H H 8.292 0.000 1 22 57 14 ASN N N 116.999 0.000 1 23 58 15 LEU H H 7.968 0.000 1 24 58 15 LEU N N 122.283 0.000 1 25 59 16 PHE H H 8.463 0.000 1 26 59 16 PHE N N 119.412 0.000 1 27 61 18 THR H H 7.661 0.000 1 28 61 18 THR N N 109.658 0.000 1 29 62 19 LEU H H 7.381 0.000 1 30 62 19 LEU N N 121.155 0.000 1 31 63 20 ALA H H 7.183 0.000 1 32 63 20 ALA N N 123.935 0.000 1 33 64 21 LYS H H 8.722 0.000 1 34 64 21 LYS N N 120.921 0.000 1 35 65 22 TRP H H 8.742 0.000 1 36 65 22 TRP N N 126.480 0.000 1 37 66 23 GLU H H 8.324 0.000 1 38 66 23 GLU N N 127.341 0.000 1 39 67 24 ASN H H 9.202 0.000 1 40 67 24 ASN N N 124.073 0.000 1 41 68 25 ASN H H 8.326 0.000 1 42 68 25 ASN N N 110.112 0.000 1 43 69 26 LYS H H 6.858 0.000 1 44 69 26 LYS N N 117.667 0.000 1 45 70 27 ILE H H 8.578 0.000 1 46 70 27 ILE N N 122.708 0.000 1 47 71 28 SER H H 8.740 0.000 1 48 71 28 SER N N 121.716 0.000 1 49 72 29 VAL H H 8.161 0.000 1 50 72 29 VAL N N 124.708 0.000 1 51 73 30 GLU H H 7.995 0.000 1 52 73 30 GLU N N 129.286 0.000 1 53 74 31 LEU H H 8.308 0.000 1 54 74 31 LEU N N 126.331 0.000 1 55 76 33 GLU H H 8.707 0.000 1 56 76 33 GLU N N 122.484 0.000 1 57 77 34 ASN H H 8.437 0.000 1 58 77 34 ASN N N 116.490 0.000 1 59 78 35 LEU H H 7.638 0.000 1 60 78 35 LEU N N 121.893 0.000 1 61 79 36 ASP H H 8.475 0.000 1 62 79 36 ASP N N 122.627 0.000 1 63 80 37 MET H H 8.209 0.000 1 64 80 37 MET N N 119.832 0.000 1 65 81 38 GLN H H 8.197 0.000 1 66 81 38 GLN N N 119.927 0.000 1 67 82 39 SER H H 7.838 0.000 1 68 82 39 SER N N 116.430 0.000 1 69 84 41 THR H H 8.154 0.000 1 70 84 41 THR N N 112.959 0.000 1 71 85 42 MET H H 8.880 0.000 1 72 85 42 MET N N 121.284 0.000 1 73 86 43 THR H H 8.010 0.000 1 74 86 43 THR N N 124.028 0.000 1 75 87 44 LEU H H 9.591 0.000 1 76 87 44 LEU N N 127.210 0.000 1 77 88 45 ILE H H 9.000 0.000 1 78 88 45 ILE N N 120.082 0.000 1 79 89 46 TYR H H 9.895 0.000 1 80 89 46 TYR N N 128.539 0.000 1 81 90 47 ASP H H 8.807 0.000 1 82 90 47 ASP N N 119.087 0.000 1 83 91 48 GLU H H 9.055 0.000 1 84 91 48 GLU N N 117.625 0.000 1 85 92 49 THR H H 8.624 0.000 1 86 92 49 THR N N 113.196 0.000 1 87 93 50 GLY H H 8.243 0.000 1 88 93 50 GLY N N 111.035 0.000 1 89 94 51 LYS H H 8.050 0.000 1 90 94 51 LYS N N 123.788 0.000 1 91 95 52 LEU H H 8.547 0.000 1 92 95 52 LEU N N 128.295 0.000 1 93 96 53 LEU H H 9.680 0.000 1 94 96 53 LEU N N 128.680 0.000 1 95 97 54 TRP H H 7.788 0.000 1 96 97 54 TRP N N 119.696 0.000 1 97 98 55 THR H H 7.405 0.000 1 98 98 55 THR N N 119.889 0.000 1 99 99 56 GLN H H 7.901 0.000 1 100 99 56 GLN N N 128.533 0.000 1 101 100 57 ARG H H 7.098 0.000 1 102 100 57 ARG N N 111.978 0.000 1 103 101 58 ASN H H 9.072 0.000 1 104 101 58 ASN N N 124.443 0.000 1 105 102 59 ILE H H 6.461 0.000 1 106 102 59 ILE N N 125.453 0.000 1 107 104 61 TRP H H 8.228 0.000 1 108 104 61 TRP N N 115.152 0.000 1 109 105 62 LEU H H 6.003 0.000 1 110 105 62 LEU N N 126.169 0.000 1 111 106 63 ILE H H 7.259 0.000 1 112 106 63 ILE N N 120.565 0.000 1 113 107 64 LYS H H 7.816 0.000 1 114 107 64 LYS N N 117.222 0.000 1 115 108 65 SER H H 7.525 0.000 1 116 108 65 SER N N 114.819 0.000 1 117 109 66 ILE H H 6.869 0.000 1 118 109 66 ILE N N 121.666 0.000 1 119 112 69 GLU H H 8.518 0.000 1 120 112 69 GLU N N 117.782 0.000 1 121 113 70 TRP H H 7.845 0.000 1 122 113 70 TRP N N 119.121 0.000 1 123 114 71 LEU H H 7.204 0.000 1 124 114 71 LEU N N 112.926 0.000 1 125 115 72 LYS H H 6.939 0.000 1 126 115 72 LYS N N 113.292 0.000 1 127 116 73 THR H H 7.297 0.000 1 128 116 73 THR N N 109.348 0.000 1 129 117 74 ASN H H 8.120 0.000 1 130 117 74 ASN N N 119.526 0.000 1 131 118 75 GLY H H 8.298 0.000 1 132 118 75 GLY N N 109.365 0.000 1 133 119 76 PHE H H 8.007 0.000 1 134 119 76 PHE N N 119.510 0.000 1 135 121 78 GLU H H 8.345 0.000 1 136 121 78 GLU N N 118.523 0.000 1 137 122 79 ILE H H 9.185 0.000 1 138 122 79 ILE N N 121.643 0.000 1 139 123 80 GLU H H 8.774 0.000 1 140 123 80 GLU N N 127.682 0.000 1 141 124 81 THR H H 9.120 0.000 1 142 124 81 THR N N 117.398 0.000 1 143 125 82 ASN H H 8.423 0.000 1 144 125 82 ASN N N 121.703 0.000 1 145 127 84 ASP H H 8.434 0.000 1 146 127 84 ASP N N 123.955 0.000 1 147 128 85 ALA H H 8.090 0.000 1 148 128 85 ALA N N 124.177 0.000 1 149 129 86 THR H H 7.993 0.000 1 150 129 86 THR N N 113.545 0.000 1 151 130 87 SER H H 8.317 0.000 1 152 130 87 SER N N 117.000 0.000 1 153 131 88 THR H H 8.102 0.000 1 154 131 88 THR N N 116.842 0.000 1 155 132 89 LEU H H 8.098 0.000 1 156 132 89 LEU N N 123.421 0.000 1 157 134 91 SER H H 7.957 0.000 1 158 134 91 SER N N 115.860 0.000 1 159 135 92 GLU H H 8.165 0.000 1 160 135 92 GLU N N 120.002 0.000 1 161 136 93 ASP H H 8.175 0.000 1 162 136 93 ASP N N 119.779 0.000 1 163 137 94 HIS H H 8.300 0.000 1 164 137 94 HIS N N 119.275 0.000 1 165 138 95 SER H H 8.461 0.000 1 166 138 95 SER N N 117.427 0.000 1 167 140 97 GLN H H 8.215 0.000 1 168 140 97 GLN N N 117.754 0.000 1 169 141 98 GLU H H 8.099 0.000 1 170 141 98 GLU N N 120.432 0.000 1 171 144 101 LYS H H 9.056 0.000 1 172 144 101 LYS N N 119.674 0.000 1 173 146 103 VAL H H 7.646 0.000 1 174 146 103 VAL N N 120.772 0.000 1 175 147 104 ARG H H 8.474 0.000 1 176 147 104 ARG N N 122.095 0.000 1 177 148 105 GLU H H 7.874 0.000 1 178 148 105 GLU N N 120.421 0.000 1 179 150 107 ASP H H 8.336 0.000 1 180 150 107 ASP N N 120.286 0.000 1 181 152 109 ASP H H 8.169 0.000 1 182 152 109 ASP N N 119.441 0.000 1 183 153 110 ALA H H 7.879 0.000 1 184 153 110 ALA N N 124.068 0.000 1 185 154 111 GLU H H 8.244 0.000 1 186 154 111 GLU N N 119.756 0.000 1 187 157 114 HIS H H 8.257 0.000 1 188 157 114 HIS N N 126.131 0.000 1 189 158 115 SER H H 9.077 0.000 1 190 158 115 SER N N 121.413 0.000 1 191 160 117 ALA H H 9.371 0.000 1 192 160 117 ALA N N 128.972 0.000 1 193 161 118 VAL H H 8.769 0.000 1 194 161 118 VAL N N 123.328 0.000 1 195 162 119 ASN H H 8.789 0.000 1 196 162 119 ASN N N 127.390 0.000 1 197 163 120 ILE H H 8.939 0.000 1 198 163 120 ILE N N 125.343 0.000 1 199 164 121 TYR H H 9.478 0.000 1 200 164 121 TYR N N 131.591 0.000 1 201 166 123 ALA H H 8.832 0.000 1 202 166 123 ALA N N 124.621 0.000 1 203 167 124 THR H H 9.161 0.000 1 204 167 124 THR N N 114.373 0.000 1 205 168 125 ALA H H 8.626 0.000 1 206 168 125 ALA N N 120.841 0.000 1 207 169 126 ARG H H 7.967 0.000 1 208 169 126 ARG N N 113.540 0.000 1 209 170 127 MET H H 7.477 0.000 1 210 170 127 MET N N 121.934 0.000 1 211 172 129 GLN H H 8.651 0.000 1 212 172 129 GLN N N 120.107 0.000 1 213 173 130 LEU H H 8.179 0.000 1 214 173 130 LEU N N 126.331 0.000 1 215 174 131 THR H H 8.974 0.000 1 216 174 131 THR N N 120.764 0.000 1 217 175 132 ILE H H 9.737 0.000 1 218 175 132 ILE N N 128.788 0.000 1 219 176 133 VAL H H 9.821 0.000 1 220 176 133 VAL N N 133.311 0.000 1 221 177 134 VAL H H 9.063 0.000 1 222 177 134 VAL N N 126.477 0.000 1 223 178 135 VAL H H 9.306 0.000 1 224 178 135 VAL N N 127.824 0.000 1 225 179 136 ASP H H 8.829 0.000 1 226 179 136 ASP N N 127.388 0.000 1 227 183 140 ILE H H 8.364 0.000 1 228 183 140 ILE N N 121.759 0.000 1 229 186 143 LYS H H 8.551 0.000 1 230 186 143 LYS N N 120.724 0.000 1 231 187 144 ARG H H 8.216 0.000 1 232 187 144 ARG N N 122.444 0.000 1 233 188 145 SER H H 8.319 0.000 1 234 188 145 SER N N 116.727 0.000 1 235 189 146 TYR H H 8.147 0.000 1 236 189 146 TYR N N 122.685 0.000 1 237 190 147 MET H H 8.017 0.000 1 238 190 147 MET N N 121.748 0.000 1 239 191 148 HIS H H 8.389 0.000 1 240 191 148 HIS N N 119.751 0.000 1 241 192 149 HIS H H 8.445 0.000 1 242 192 149 HIS N N 119.608 0.000 1 243 193 150 HIS H H 8.641 0.000 1 244 193 150 HIS N N 120.515 0.000 1 245 194 151 HIS H H 8.708 0.000 1 246 194 151 HIS N N 121.094 0.000 1 247 195 152 HIS H H 8.652 0.000 1 248 195 152 HIS N N 121.301 0.000 1 249 196 153 HIS H H 8.208 0.000 1 250 196 153 HIS N N 126.109 0.000 1 stop_ save_