data_50204 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Musashi-1 C-terminal domain, residues 194-362 ; _BMRB_accession_number 50204 _BMRB_flat_file_name bmr50204.str _Entry_type original _Submission_date 2020-02-24 _Accession_date 2020-02-24 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Chen Tsai-chen . . 2 Huang Jie-rong . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 145 "13C chemical shifts" 462 "15N chemical shifts" 145 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-06-09 original BMRB . stop_ _Original_release_date 2020-02-25 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Musashi-1: An Example of How Polyalanine Tracts Contribute to Self-Association in the Intrinsically Disordered Regions of RNA-Binding Proteins ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 32225071 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Chen Tsai-chen . . 2 Huang Jie-rong . . stop_ _Journal_abbreviation 'Int. J. Mol. Sci.' _Journal_name_full 'International journal of molecular sciences' _Journal_volume 21 _Journal_issue 7 _Journal_ISSN 1422-0067 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2289 _Page_last 2289 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly_1 _Saveframe_category molecular_system _Mol_system_name Musashi-1 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Musashi-1 $entity_1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Musashi-1 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 169 _Mol_residue_sequence ; GSARGRSRVMPYGMDAFMLG IGMLGYPGFQATTYASRSYT GLAPGYTYQFPEFRVERSPL PSAPVLPELTAIPLTAYGPM AAAAAAAAVVRGTGSHPWTM APPPGSTPSRTGGFLGTTSP GPMAELYGAANQDSGVSSYI SAASPAPSTGFGHSLGGPLI ATAFTNGYH ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 194 GLY 2 195 SER 3 196 ALA 4 197 ARG 5 198 GLY 6 199 ARG 7 200 SER 8 201 ARG 9 202 VAL 10 203 MET 11 204 PRO 12 205 TYR 13 206 GLY 14 207 MET 15 208 ASP 16 209 ALA 17 210 PHE 18 211 MET 19 212 LEU 20 213 GLY 21 214 ILE 22 215 GLY 23 216 MET 24 217 LEU 25 218 GLY 26 219 TYR 27 220 PRO 28 221 GLY 29 222 PHE 30 223 GLN 31 224 ALA 32 225 THR 33 226 THR 34 227 TYR 35 228 ALA 36 229 SER 37 230 ARG 38 231 SER 39 232 TYR 40 233 THR 41 234 GLY 42 235 LEU 43 236 ALA 44 237 PRO 45 238 GLY 46 239 TYR 47 240 THR 48 241 TYR 49 242 GLN 50 243 PHE 51 244 PRO 52 245 GLU 53 246 PHE 54 247 ARG 55 248 VAL 56 249 GLU 57 250 ARG 58 251 THR 59 252 PRO 60 253 LEU 61 254 PRO 62 255 SER 63 256 ALA 64 257 PRO 65 258 VAL 66 259 LEU 67 260 PRO 68 261 GLU 69 262 LEU 70 263 THR 71 264 ALA 72 265 ILE 73 266 PRO 74 267 LEU 75 268 THR 76 269 ALA 77 270 TYR 78 271 GLY 79 272 PRO 80 273 MET 81 274 ALA 82 275 ALA 83 276 ALA 84 277 ALA 85 278 ALA 86 279 ALA 87 280 ALA 88 281 ALA 89 282 VAL 90 283 VAL 91 284 ARG 92 285 GLY 93 286 THR 94 287 GLY 95 288 SER 96 289 HIS 97 290 PRO 98 291 TRP 99 292 THR 100 293 MET 101 294 ALA 102 295 PRO 103 296 PRO 104 297 PRO 105 298 GLY 106 299 SER 107 300 THR 108 301 PRO 109 302 SER 110 303 ARG 111 304 THR 112 305 GLY 113 306 GLY 114 307 PHE 115 308 LEU 116 309 GLY 117 310 THR 118 311 THR 119 312 SER 120 313 PRO 121 314 GLY 122 315 PRO 123 316 MET 124 317 ALA 125 318 GLU 126 319 LEU 127 320 TYR 128 321 GLY 129 322 ALA 130 323 ALA 131 324 ASN 132 325 GLN 133 326 ASP 134 327 SER 135 328 GLY 136 329 VAL 137 330 SER 138 331 SER 139 332 TYR 140 333 ILE 141 334 SER 142 335 ALA 143 336 ALA 144 337 SER 145 338 PRO 146 339 ALA 147 340 PRO 148 341 SER 149 342 THR 150 343 GLY 151 344 PHE 152 345 GLY 153 346 HIS 154 347 SER 155 348 LEU 156 349 GLY 157 350 GLY 158 351 PRO 159 352 LEU 160 353 ILE 161 354 ALA 162 355 THR 163 356 ALA 164 357 PHE 165 358 THR 166 359 ASN 167 360 GLY 168 361 TYR 169 362 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source_1 _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . . . . plasmid pET21 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 20-100 uM '[U-99% 15N]' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name SPARKY _Version . loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_(H)N(COCO)NH_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D (H)N(COCO)NH' _Sample_label $sample_1 save_ save_3D_(HN)CO(CO)NH_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D (HN)CO(CO)NH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 carbon ppm 0 na indirect . . . 1 water H 1 protons ppm 0 internal direct . . . 1 water N 15 nitrogen ppm 0 na indirect . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $software_1 stop_ loop_ _Experiment_label '3D HNCACB' '3D HN(CO)CA' '3D (H)N(COCO)NH' '3D (HN)CO(CO)NH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name Musashi-1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 194 1 GLY H H 8.479 . . 2 194 1 GLY C C 173.819 . . 3 194 1 GLY CA C 45.006 . . 4 194 1 GLY N N 110.654 . . 5 195 2 SER H H 8.318 . . 6 195 2 SER C C 174.313 . . 7 195 2 SER CA C 58.085 . . 8 195 2 SER CB C 64.004 . . 9 195 2 SER N N 115.761 . . 10 196 3 ALA H H 8.457 . . 11 196 3 ALA C C 177.669 . . 12 196 3 ALA CA C 52.533 . . 13 196 3 ALA CB C 19.042 . . 14 196 3 ALA N N 126.252 . . 15 197 4 ARG H H 8.338 . . 16 197 4 ARG C C 176.789 . . 17 197 4 ARG CA C 56.145 . . 18 197 4 ARG CB C 30.696 . . 19 197 4 ARG N N 120.460 . . 20 198 5 GLY H H 8.376 . . 21 198 5 GLY C C 173.838 . . 22 198 5 GLY CA C 45.049 . . 23 198 5 GLY N N 110.044 . . 24 199 6 ARG H H 8.235 . . 25 199 6 ARG C C 176.342 . . 26 199 6 ARG CA C 55.938 . . 27 199 6 ARG CB C 30.799 . . 28 199 6 ARG N N 120.578 . . 29 200 7 SER H H 8.340 . . 30 200 7 SER C C 174.217 . . 31 200 7 SER CA C 58.231 . . 32 200 7 SER CB C 63.874 . . 33 200 7 SER N N 117.318 . . 34 201 8 ARG H H 8.413 . . 35 201 8 ARG C C 175.833 . . 36 201 8 ARG CA C 56.119 . . 37 201 8 ARG CB C 30.758 . . 38 201 8 ARG N N 123.025 . . 39 202 9 VAL H H 8.149 . . 40 202 9 VAL C C 175.723 . . 41 202 9 VAL CA C 62.279 . . 42 202 9 VAL CB C 32.520 . . 43 202 9 VAL N N 121.367 . . 44 203 10 MET H H 8.364 . . 45 203 10 MET C C 174.087 . . 46 203 10 MET CA C 52.736 . . 47 203 10 MET CB C 32.374 . . 48 203 10 MET N N 125.521 . . 49 204 11 PRO C C 176.092 . . 50 204 11 PRO CA C 63.067 . . 51 204 11 PRO CB C 31.881 . . 52 205 12 TYR H H 8.181 . . 53 205 12 TYR C C 176.271 . . 54 205 12 TYR CA C 58.018 . . 55 205 12 TYR CB C 38.570 . . 56 205 12 TYR N N 119.824 . . 57 206 13 GLY H H 8.230 . . 58 206 13 GLY C C 174.316 . . 59 206 13 GLY CA C 45.149 . . 60 206 13 GLY N N 110.905 . . 61 207 14 MET H H 8.108 . . 62 207 14 MET C C 176.096 . . 63 207 14 MET CA C 55.819 . . 64 207 14 MET CB C 32.523 . . 65 207 14 MET N N 119.738 . . 66 208 15 ASP H H 8.291 . . 67 208 15 ASP C C 176.431 . . 68 208 15 ASP CA C 54.819 . . 69 208 15 ASP CB C 40.898 . . 70 208 15 ASP N N 120.403 . . 71 209 16 ALA H H 8.062 . . 72 209 16 ALA C C 178.275 . . 73 209 16 ALA CA C 53.525 . . 74 209 16 ALA CB C 18.616 . . 75 209 16 ALA N N 122.840 . . 76 210 17 PHE H H 8.014 . . 77 210 17 PHE C C 176.473 . . 78 210 17 PHE CB C 39.047 . . 79 210 17 PHE N N 118.164 . . 80 211 18 MET H H 8.017 . . 81 211 18 MET C C 175.085 . . 82 211 18 MET N N 119.721 . . 83 212 19 LEU H H 7.945 . . 84 212 19 LEU C C 177.810 . . 85 212 19 LEU CA C 55.217 . . 86 212 19 LEU N N 121.875 . . 87 213 20 GLY H H 8.031 . . 88 213 20 GLY C C 174.205 . . 89 213 20 GLY CA C 45.380 . . 90 213 20 GLY N N 108.570 . . 91 214 21 ILE H H 7.773 . . 92 214 21 ILE C C 176.803 . . 93 214 21 ILE CA C 61.645 . . 94 214 21 ILE N N 119.044 . . 95 215 22 GLY H H 8.310 . . 96 215 22 GLY C C 174.184 . . 97 215 22 GLY CA C 45.422 . . 98 215 22 GLY N N 111.882 . . 99 216 23 MET H H 7.969 . . 100 216 23 MET C C 175.397 . . 101 216 23 MET CA C 55.325 . . 102 216 23 MET CB C 32.852 . . 103 216 23 MET N N 119.205 . . 104 217 24 LEU C C 177.508 . . 105 218 25 GLY H H 8.219 . . 106 218 25 GLY C C 173.258 . . 107 218 25 GLY CA C 44.840 . . 108 218 25 GLY N N 109.036 . . 109 219 26 TYR H H 7.878 . . 110 219 26 TYR C C 174.144 . . 111 219 26 TYR CA C 55.881 . . 112 219 26 TYR CB C 38.008 . . 113 219 26 TYR N N 121.015 . . 114 220 27 PRO C C 177.166 . . 115 220 27 PRO CA C 63.774 . . 116 220 27 PRO CB C 31.675 . . 117 221 28 GLY H H 7.975 . . 118 221 28 GLY C C 173.895 . . 119 221 28 GLY CA C 45.160 . . 120 221 28 GLY N N 108.794 . . 121 222 29 PHE H H 7.999 . . 122 222 29 PHE C C 175.524 . . 123 222 29 PHE CA C 58.275 . . 124 222 29 PHE CB C 39.489 . . 125 222 29 PHE N N 120.327 . . 126 223 30 GLN H H 8.189 . . 127 223 30 GLN C C 174.981 . . 128 223 30 GLN CA C 55.425 . . 129 223 30 GLN CB C 29.498 . . 130 223 30 GLN N N 122.706 . . 131 224 31 ALA H H 8.231 . . 132 224 31 ALA C C 177.961 . . 133 224 31 ALA CA C 52.758 . . 134 224 31 ALA CB C 19.150 . . 135 224 31 ALA N N 125.211 . . 136 225 32 THR H H 8.083 . . 137 225 32 THR C C 174.723 . . 138 225 32 THR CA C 62.023 . . 139 225 32 THR CB C 69.716 . . 140 225 32 THR N N 112.755 . . 141 226 33 THR H H 7.964 . . 142 226 33 THR C C 174.281 . . 143 226 33 THR CA C 61.908 . . 144 226 33 THR CB C 69.725 . . 145 226 33 THR N N 115.626 . . 146 227 34 TYR H H 8.114 . . 147 227 34 TYR C C 175.716 . . 148 227 34 TYR CA C 58.229 . . 149 227 34 TYR CB C 38.581 . . 150 227 34 TYR N N 122.010 . . 151 228 35 ALA H H 8.119 . . 152 228 35 ALA C C 177.545 . . 153 228 35 ALA CA C 52.635 . . 154 228 35 ALA CB C 19.112 . . 155 228 35 ALA N N 124.590 . . 156 229 36 SER H H 8.104 . . 157 229 36 SER C C 174.636 . . 158 229 36 SER CA C 58.551 . . 159 229 36 SER CB C 63.656 . . 160 229 36 SER N N 114.563 . . 161 230 37 ARG H H 8.202 . . 162 230 37 ARG C C 175.981 . . 163 230 37 ARG CA C 56.164 . . 164 230 37 ARG CB C 30.557 . . 165 230 37 ARG N N 122.332 . . 166 231 38 SER H H 8.165 . . 167 231 38 SER C C 173.963 . . 168 231 38 SER CA C 58.124 . . 169 231 38 SER CB C 63.766 . . 170 231 38 SER N N 115.968 . . 171 232 39 TYR H H 8.189 . . 172 232 39 TYR C C 175.900 . . 173 232 39 TYR CA C 57.909 . . 174 232 39 TYR CB C 38.758 . . 175 232 39 TYR N N 122.287 . . 176 233 40 THR H H 8.077 . . 177 233 40 THR C C 174.695 . . 178 233 40 THR CA C 61.763 . . 179 233 40 THR CB C 69.693 . . 180 233 40 THR N N 115.400 . . 181 234 41 GLY H H 7.715 . . 182 234 41 GLY C C 173.450 . . 183 234 41 GLY CA C 45.090 . . 184 234 41 GLY N N 110.435 . . 185 235 42 LEU H H 7.957 . . 186 235 42 LEU C C 176.726 . . 187 235 42 LEU CA C 54.534 . . 188 235 42 LEU CB C 42.450 . . 189 235 42 LEU N N 121.039 . . 190 236 43 ALA H H 8.251 . . 191 236 43 ALA C C 175.186 . . 192 236 43 ALA CA C 50.434 . . 193 236 43 ALA CB C 17.997 . . 194 236 43 ALA N N 126.176 . . 195 237 44 PRO C C 177.419 . . 196 237 44 PRO CA C 63.439 . . 197 237 44 PRO CB C 31.831 . . 198 238 45 GLY H H 8.414 . . 199 238 45 GLY C C 173.837 . . 200 238 45 GLY CA C 45.125 . . 201 238 45 GLY N N 109.209 . . 202 239 46 TYR H H 7.881 . . 203 239 46 TYR C C 175.663 . . 204 239 46 TYR CA C 58.130 . . 205 239 46 TYR CB C 39.073 . . 206 239 46 TYR N N 119.925 . . 207 240 47 THR H H 7.943 . . 208 240 47 THR C C 173.549 . . 209 240 47 THR CA C 61.461 . . 210 240 47 THR CB C 70.096 . . 211 240 47 THR N N 115.668 . . 212 241 48 TYR H H 8.010 . . 213 241 48 TYR CA C 58.059 . . 214 241 48 TYR N N 122.313 . . 215 244 51 PRO C C 176.530 . . 216 244 51 PRO CA C 63.337 . . 217 244 51 PRO CB C 31.864 . . 218 245 52 GLU H H 8.501 . . 219 245 52 GLU C C 175.992 . . 220 245 52 GLU CA C 56.624 . . 221 245 52 GLU CB C 30.040 . . 222 245 52 GLU N N 120.300 . . 223 246 53 PHE H H 8.138 . . 224 246 53 PHE C C 175.093 . . 225 246 53 PHE CA C 57.468 . . 226 246 53 PHE CB C 39.492 . . 227 246 53 PHE N N 121.040 . . 228 247 54 ARG H H 8.083 . . 229 247 54 ARG C C 175.389 . . 230 247 54 ARG CA C 55.595 . . 231 247 54 ARG CB C 31.045 . . 232 247 54 ARG N N 123.456 . . 233 248 55 VAL H H 8.175 . . 234 248 55 VAL C C 175.926 . . 235 248 55 VAL CA C 62.234 . . 236 248 55 VAL CB C 32.604 . . 237 248 55 VAL N N 122.243 . . 238 249 56 GLU H H 8.544 . . 239 249 56 GLU C C 176.039 . . 240 249 56 GLU CA C 56.182 . . 241 249 56 GLU CB C 30.211 . . 242 249 56 GLU N N 125.257 . . 243 250 57 ARG H H 8.423 . . 244 250 57 ARG C C 176.007 . . 245 250 57 ARG CA C 55.762 . . 246 250 57 ARG CB C 30.730 . . 247 250 57 ARG N N 122.624 . . 248 251 58 THR H H 8.232 . . 249 251 58 THR C C 172.539 . . 250 251 58 THR CA C 59.916 . . 251 251 58 THR CB C 69.638 . . 252 251 58 THR N N 118.662 . . 253 252 59 PRO C C 176.537 . . 254 252 59 PRO CA C 62.887 . . 255 252 59 PRO CB C 32.054 . . 256 253 60 LEU H H 8.357 . . 257 253 60 LEU C C 175.437 . . 258 253 60 LEU CA C 53.009 . . 259 253 60 LEU CB C 41.444 . . 260 253 60 LEU N N 123.993 . . 261 254 61 PRO C C 176.731 . . 262 254 61 PRO CA C 63.119 . . 263 254 61 PRO CB C 31.897 . . 264 255 62 SER H H 8.277 . . 265 255 62 SER C C 173.660 . . 266 255 62 SER CA C 58.032 . . 267 255 62 SER CB C 63.747 . . 268 255 62 SER N N 115.759 . . 269 256 63 ALA H H 8.196 . . 270 256 63 ALA C C 175.186 . . 271 256 63 ALA CA C 50.432 . . 272 256 63 ALA CB C 18.199 . . 273 256 63 ALA N N 126.933 . . 274 257 64 PRO C C 176.458 . . 275 257 64 PRO CA C 62.838 . . 276 257 64 PRO CB C 31.954 . . 277 258 65 VAL H H 8.286 . . 278 258 65 VAL C C 175.976 . . 279 258 65 VAL CA C 62.082 . . 280 258 65 VAL CB C 32.608 . . 281 258 65 VAL N N 121.356 . . 282 259 66 LEU H H 8.372 . . 283 259 66 LEU C C 175.034 . . 284 259 66 LEU CA C 52.708 . . 285 259 66 LEU CB C 41.435 . . 286 259 66 LEU N N 128.212 . . 287 260 67 PRO C C 176.730 . . 288 260 67 PRO CA C 62.988 . . 289 260 67 PRO CB C 31.919 . . 290 261 68 GLU H H 8.530 . . 291 261 68 GLU C C 176.532 . . 292 261 68 GLU CA C 56.429 . . 293 261 68 GLU CB C 30.024 . . 294 261 68 GLU N N 120.806 . . 295 262 69 LEU H H 8.336 . . 296 262 69 LEU C C 177.387 . . 297 262 69 LEU CA C 55.173 . . 298 262 69 LEU CB C 42.042 . . 299 262 69 LEU N N 123.570 . . 300 263 70 THR H H 8.028 . . 301 263 70 THR C C 173.860 . . 302 263 70 THR CA C 61.788 . . 303 263 70 THR CB C 69.707 . . 304 263 70 THR N N 114.897 . . 305 264 71 ALA H H 8.224 . . 306 264 71 ALA C C 177.160 . . 307 264 71 ALA CA C 52.106 . . 308 264 71 ALA CB C 19.182 . . 309 264 71 ALA N N 126.731 . . 310 265 72 ILE H H 8.140 . . 311 265 72 ILE C C 174.518 . . 312 265 72 ILE CA C 58.669 . . 313 265 72 ILE CB C 38.500 . . 314 265 72 ILE N N 122.479 . . 315 266 73 PRO C C 176.777 . . 316 266 73 PRO CA C 62.927 . . 317 266 73 PRO CB C 32.077 . . 318 267 74 LEU H H 8.401 . . 319 267 74 LEU C C 177.792 . . 320 267 74 LEU CA C 55.570 . . 321 267 74 LEU CB C 42.019 . . 322 267 74 LEU N N 122.591 . . 323 268 75 THR H H 7.912 . . 324 268 75 THR C C 174.081 . . 325 268 75 THR CA C 61.694 . . 326 268 75 THR CB C 69.707 . . 327 268 75 THR N N 113.468 . . 328 269 76 ALA H H 8.154 . . 329 269 76 ALA C C 176.955 . . 330 269 76 ALA CA C 52.348 . . 331 269 76 ALA CB C 19.192 . . 332 269 76 ALA N N 125.635 . . 333 270 77 TYR H H 7.999 . . 334 270 77 TYR C C 176.040 . . 335 270 77 TYR CA C 57.803 . . 336 270 77 TYR CB C 38.996 . . 337 270 77 TYR N N 118.801 . . 338 271 78 GLY H H 8.155 . . 339 271 78 GLY C C 172.363 . . 340 271 78 GLY CA C 45.018 . . 341 271 78 GLY N N 110.154 . . 342 272 79 PRO C C 178.068 . . 343 272 79 PRO CA C 63.967 . . 344 272 79 PRO CB C 31.912 . . 345 273 80 MET H H 8.354 . . 346 273 80 MET C C 177.006 . . 347 273 80 MET CA C 56.370 . . 348 273 80 MET CB C 32.202 . . 349 273 80 MET N N 119.278 . . 350 274 81 ALA H H 8.107 . . 351 274 81 ALA C C 178.697 . . 352 274 81 ALA CA C 53.373 . . 353 274 81 ALA CB C 18.552 . . 354 274 81 ALA N N 124.858 . . 355 275 82 ALA H H 8.222 . . 356 275 82 ALA C C 178.961 . . 357 275 82 ALA CA C 53.537 . . 358 275 82 ALA CB C 18.455 . . 359 275 82 ALA N N 122.700 . . 360 276 83 ALA H H 8.114 . . 361 276 83 ALA C C 178.963 . . 362 276 83 ALA CA C 53.474 . . 363 276 83 ALA CB C 18.382 . . 364 276 83 ALA N N 122.463 . . 365 277 84 ALA H H 8.015 . . 366 277 84 ALA C C 178.887 . . 367 277 84 ALA CA C 53.401 . . 368 277 84 ALA CB C 18.442 . . 369 277 84 ALA N N 122.543 . . 370 278 85 ALA H H 8.050 . . 371 278 85 ALA C C 178.725 . . 372 278 85 ALA CA C 53.383 . . 373 278 85 ALA CB C 18.419 . . 374 278 85 ALA N N 122.360 . . 375 279 86 ALA H H 7.971 . . 376 279 86 ALA C C 178.510 . . 377 279 86 ALA CA C 53.244 . . 378 279 86 ALA CB C 18.469 . . 379 279 86 ALA N N 121.844 . . 380 280 87 ALA H H 7.921 . . 381 280 87 ALA C C 178.038 . . 382 280 87 ALA CA C 52.904 . . 383 280 87 ALA CB C 18.647 . . 384 280 87 ALA N N 121.585 . . 385 281 88 ALA H H 7.881 . . 386 281 88 ALA C C 178.213 . . 387 281 88 ALA CA C 52.874 . . 388 281 88 ALA CB C 18.723 . . 389 281 88 ALA N N 121.877 . . 390 282 89 VAL H H 7.798 . . 391 282 89 VAL C C 176.652 . . 392 282 89 VAL CA C 63.120 . . 393 282 89 VAL CB C 32.328 . . 394 282 89 VAL N N 119.202 . . 395 283 90 VAL H H 8.034 . . 396 283 90 VAL C C 176.376 . . 397 283 90 VAL CA C 62.902 . . 398 283 90 VAL CB C 32.287 . . 399 283 90 VAL N N 123.871 . . 400 284 91 ARG H H 8.336 . . 401 284 91 ARG C C 176.786 . . 402 284 91 ARG CA C 56.380 . . 403 284 91 ARG CB C 30.725 . . 404 284 91 ARG N N 124.522 . . 405 285 92 GLY H H 8.289 . . 406 285 92 GLY C C 174.357 . . 407 285 92 GLY CA C 45.162 . . 408 285 92 GLY N N 109.550 . . 409 286 93 THR H H 8.139 . . 410 286 93 THR C C 175.267 . . 411 286 93 THR CA C 61.911 . . 412 286 93 THR CB C 69.797 . . 413 286 93 THR N N 113.055 . . 414 287 94 GLY H H 8.473 . . 415 287 94 GLY C C 173.942 . . 416 287 94 GLY CA C 45.175 . . 417 287 94 GLY N N 110.983 . . 418 288 95 SER H H 8.124 . . 419 288 95 SER C C 173.831 . . 420 288 95 SER CA C 58.145 . . 421 288 95 SER CB C 63.687 . . 422 288 95 SER N N 115.145 . . 423 289 96 HIS H H 8.339 . . 424 289 96 HIS C C 172.430 . . 425 289 96 HIS CA C 53.103 . . 426 289 96 HIS CB C 28.591 . . 427 289 96 HIS N N 120.143 . . 428 290 97 PRO C C 176.508 . . 429 290 97 PRO CA C 63.457 . . 430 290 97 PRO CB C 31.825 . . 431 291 98 TRP H H 8.065 . . 432 291 98 TRP C C 176.153 . . 433 291 98 TRP CA C 57.379 . . 434 291 98 TRP CB C 29.152 . . 435 291 98 TRP N N 120.363 . . 436 292 99 THR H H 7.816 . . 437 292 99 THR C C 173.565 . . 438 292 99 THR CA C 61.536 . . 439 292 99 THR CB C 69.916 . . 440 292 99 THR N N 116.164 . . 441 293 100 MET H H 8.052 . . 442 293 100 MET C C 175.162 . . 443 293 100 MET CA C 55.077 . . 444 293 100 MET CB C 32.947 . . 445 293 100 MET N N 122.479 . . 446 294 101 ALA H H 8.262 . . 447 294 101 ALA C C 174.694 . . 448 294 101 ALA CA C 50.331 . . 449 294 101 ALA CB C 17.906 . . 450 294 101 ALA N N 127.062 . . 451 297 104 PRO C C 177.549 . . 452 297 104 PRO CA C 63.344 . . 453 297 104 PRO CB C 31.832 . . 454 298 105 GLY H H 8.557 . . 455 298 105 GLY C C 174.181 . . 456 298 105 GLY CA C 45.123 . . 457 298 105 GLY N N 109.892 . . 458 299 106 SER H H 8.063 . . 459 299 106 SER C C 174.183 . . 460 299 106 SER CA C 58.371 . . 461 299 106 SER CB C 63.988 . . 462 299 106 SER N N 115.337 . . 463 300 107 THR H H 8.272 . . 464 300 107 THR C C 172.715 . . 465 300 107 THR CA C 59.827 . . 466 300 107 THR CB C 69.718 . . 467 300 107 THR N N 118.193 . . 468 301 108 PRO C C 176.811 . . 469 301 108 PRO CA C 63.248 . . 470 301 108 PRO CB C 32.049 . . 471 302 109 SER H H 8.419 . . 472 302 109 SER C C 174.745 . . 473 302 109 SER CA C 58.229 . . 474 302 109 SER CB C 63.715 . . 475 302 109 SER N N 116.295 . . 476 303 110 ARG H H 8.492 . . 477 303 110 ARG C C 176.478 . . 478 303 110 ARG CA C 56.137 . . 479 303 110 ARG CB C 30.696 . . 480 303 110 ARG N N 123.419 . . 481 304 111 THR H H 8.177 . . 482 304 111 THR C C 175.013 . . 483 304 111 THR CA C 61.802 . . 484 304 111 THR CB C 69.675 . . 485 304 111 THR N N 114.431 . . 486 305 112 GLY H H 8.370 . . 487 305 112 GLY C C 174.355 . . 488 305 112 GLY CA C 45.262 . . 489 305 112 GLY N N 111.130 . . 490 306 113 GLY H H 8.195 . . 491 306 113 GLY C C 173.692 . . 492 306 113 GLY CA C 44.910 . . 493 306 113 GLY N N 108.475 . . 494 307 114 PHE H H 8.159 . . 495 307 114 PHE C C 175.828 . . 496 307 114 PHE CA C 57.823 . . 497 307 114 PHE CB C 39.492 . . 498 307 114 PHE N N 120.161 . . 499 308 115 LEU H H 8.304 . . 500 308 115 LEU C C 177.368 . . 501 308 115 LEU CA C 55.006 . . 502 308 115 LEU CB C 42.236 . . 503 308 115 LEU N N 124.532 . . 504 309 116 GLY H H 7.834 . . 505 309 116 GLY C C 174.007 . . 506 309 116 GLY CA C 45.214 . . 507 309 116 GLY N N 109.020 . . 508 310 117 THR H H 8.049 . . 509 310 117 THR C C 174.687 . . 510 310 117 THR CA C 61.595 . . 511 310 117 THR CB C 69.848 . . 512 310 117 THR N N 113.250 . . 513 311 118 THR H H 8.209 . . 514 311 118 THR C C 174.168 . . 515 311 118 THR CA C 61.650 . . 516 311 118 THR CB C 69.818 . . 517 311 118 THR N N 116.196 . . 518 312 119 SER H H 8.360 . . 519 312 119 SER C C 172.574 . . 520 312 119 SER CA C 56.299 . . 521 312 119 SER CB C 63.302 . . 522 312 119 SER N N 119.742 . . 523 313 120 PRO C C 176.956 . . 524 313 120 PRO CA C 63.349 . . 525 313 120 PRO CB C 32.097 . . 526 314 121 GLY H H 8.196 . . 527 314 121 GLY C C 171.716 . . 528 314 121 GLY CA C 44.454 . . 529 314 121 GLY N N 109.122 . . 530 315 122 PRO C C 177.362 . . 531 315 122 PRO CA C 63.309 . . 532 315 122 PRO CB C 31.950 . . 533 316 123 MET H H 8.451 . . 534 316 123 MET C C 176.152 . . 535 316 123 MET CA C 55.527 . . 536 316 123 MET CB C 32.455 . . 537 316 123 MET N N 120.063 . . 538 317 124 ALA H H 8.160 . . 539 317 124 ALA C C 177.875 . . 540 317 124 ALA CA C 52.879 . . 541 317 124 ALA CB C 19.105 . . 542 317 124 ALA N N 124.642 . . 543 318 125 GLU H H 8.302 . . 544 318 125 GLU C C 176.381 . . 545 318 125 GLU CA C 56.579 . . 546 318 125 GLU CB C 29.888 . . 547 318 125 GLU N N 119.496 . . 548 319 126 LEU C C 177.148 . . 549 319 126 LEU CA C 55.254 . . 550 319 126 LEU CB C 42.245 . . 551 320 127 TYR H H 8.116 . . 552 320 127 TYR C C 176.398 . . 553 320 127 TYR CA C 57.902 . . 554 320 127 TYR CB C 38.472 . . 555 320 127 TYR N N 119.965 . . 556 321 128 GLY H H 8.198 . . 557 321 128 GLY C C 173.763 . . 558 321 128 GLY CA C 45.198 . . 559 321 128 GLY N N 110.309 . . 560 322 129 ALA H H 8.054 . . 561 322 129 ALA C C 177.591 . . 562 322 129 ALA CA C 52.469 . . 563 322 129 ALA CB C 19.234 . . 564 322 129 ALA N N 123.642 . . 565 323 130 ALA H H 8.266 . . 566 323 130 ALA C C 177.533 . . 567 323 130 ALA CA C 52.509 . . 568 323 130 ALA CB C 18.984 . . 569 323 130 ALA N N 122.508 . . 570 324 131 ASN H H 8.286 . . 571 324 131 ASN C C 175.242 . . 572 324 131 ASN CA C 53.243 . . 573 324 131 ASN CB C 38.671 . . 574 324 131 ASN N N 117.368 . . 575 325 132 GLN H H 8.327 . . 576 325 132 GLN C C 175.667 . . 577 325 132 GLN CA C 55.951 . . 578 325 132 GLN CB C 29.294 . . 579 325 132 GLN N N 120.497 . . 580 326 133 ASP H H 8.377 . . 581 326 133 ASP C C 176.365 . . 582 326 133 ASP CA C 54.354 . . 583 326 133 ASP CB C 41.076 . . 584 326 133 ASP N N 121.438 . . 585 327 134 SER H H 8.307 . . 586 327 134 SER C C 175.147 . . 587 327 134 SER CA C 58.771 . . 588 327 134 SER CB C 63.684 . . 589 327 134 SER N N 116.771 . . 590 328 135 GLY H H 8.455 . . 591 328 135 GLY C C 174.173 . . 592 328 135 GLY CA C 45.320 . . 593 328 135 GLY N N 110.761 . . 594 329 136 VAL H H 7.894 . . 595 329 136 VAL C C 176.374 . . 596 329 136 VAL CA C 62.319 . . 597 329 136 VAL CB C 32.570 . . 598 329 136 VAL N N 119.095 . . 599 330 137 SER H H 8.414 . . 600 330 137 SER C C 174.534 . . 601 330 137 SER CA C 58.393 . . 602 330 137 SER CB C 63.703 . . 603 330 137 SER N N 119.359 . . 604 331 138 SER H H 8.250 . . 605 331 138 SER C C 173.913 . . 606 331 138 SER CA C 58.389 . . 607 331 138 SER CB C 63.703 . . 608 331 138 SER N N 117.897 . . 609 332 139 TYR H H 8.094 . . 610 332 139 TYR C C 175.403 . . 611 332 139 TYR CA C 58.037 . . 612 332 139 TYR CB C 38.582 . . 613 332 139 TYR N N 122.106 . . 614 333 140 ILE H H 7.918 . . 615 333 140 ILE C C 175.723 . . 616 333 140 ILE CA C 60.730 . . 617 333 140 ILE CB C 38.639 . . 618 333 140 ILE N N 123.516 . . 619 334 141 SER H H 8.260 . . 620 334 141 SER C C 174.243 . . 621 334 141 SER CA C 58.120 . . 622 334 141 SER CB C 63.793 . . 623 334 141 SER N N 120.076 . . 624 335 142 ALA H H 8.307 . . 625 335 142 ALA C C 177.162 . . 626 335 142 ALA CA C 52.388 . . 627 335 142 ALA CB C 19.146 . . 628 335 142 ALA N N 126.340 . . 629 336 143 ALA H H 8.166 . . 630 336 143 ALA C C 177.469 . . 631 336 143 ALA CA C 52.162 . . 632 336 143 ALA CB C 19.185 . . 633 336 143 ALA N N 122.880 . . 634 337 144 SER H H 8.204 . . 635 337 144 SER C C 172.284 . . 636 337 144 SER CA C 56.329 . . 637 337 144 SER CB C 63.159 . . 638 337 144 SER N N 116.767 . . 639 338 145 PRO C C 176.220 . . 640 338 145 PRO CA C 62.853 . . 641 338 145 PRO CB C 31.953 . . 642 339 146 ALA H H 8.404 . . 643 339 146 ALA C C 175.527 . . 644 339 146 ALA CA C 50.384 . . 645 339 146 ALA CB C 17.829 . . 646 339 146 ALA N N 125.991 . . 647 340 147 PRO C C 177.010 . . 648 340 147 PRO CA C 62.978 . . 649 340 147 PRO CB C 31.979 . . 650 341 148 SER H H 8.492 . . 651 341 148 SER C C 174.890 . . 652 341 148 SER CA C 58.285 . . 653 341 148 SER CB C 63.625 . . 654 341 148 SER N N 116.281 . . 655 342 149 THR H H 8.105 . . 656 342 149 THR C C 174.936 . . 657 342 149 THR CA C 61.728 . . 658 342 149 THR CB C 69.741 . . 659 342 149 THR N N 114.812 . . 660 343 150 GLY H H 8.273 . . 661 343 150 GLY C C 173.769 . . 662 343 150 GLY CA C 45.021 . . 663 343 150 GLY N N 110.337 . . 664 344 151 PHE H H 8.179 . . 665 344 151 PHE C C 176.432 . . 666 344 151 PHE CA C 57.969 . . 667 344 151 PHE CB C 39.410 . . 668 344 151 PHE N N 120.022 . . 669 345 152 GLY H H 8.407 . . 670 345 152 GLY C C 173.803 . . 671 345 152 GLY CA C 45.182 . . 672 345 152 GLY N N 110.617 . . 673 346 153 HIS H H 8.239 . . 674 346 153 HIS C C 174.395 . . 675 346 153 HIS CA C 55.094 . . 676 346 153 HIS CB C 29.181 . . 677 346 153 HIS N N 118.078 . . 678 347 154 SER H H 8.403 . . 679 347 154 SER C C 174.510 . . 680 347 154 SER CA C 58.252 . . 681 347 154 SER CB C 63.726 . . 682 347 154 SER N N 117.226 . . 683 348 155 LEU H H 8.479 . . 684 348 155 LEU C C 177.763 . . 685 348 155 LEU CA C 55.287 . . 686 348 155 LEU CB C 42.105 . . 687 348 155 LEU N N 124.445 . . 688 349 156 GLY H H 8.382 . . 689 349 156 GLY C C 174.081 . . 690 349 156 GLY CA C 45.012 . . 691 349 156 GLY N N 109.553 . . 692 350 157 GLY H H 8.069 . . 693 350 157 GLY C C 171.626 . . 694 350 157 GLY CA C 44.382 . . 695 350 157 GLY N N 108.896 . . 696 351 158 PRO C C 176.764 . . 697 351 158 PRO CA C 62.929 . . 698 351 158 PRO CB C 32.104 . . 699 352 159 LEU H H 8.332 . . 700 352 159 LEU C C 177.175 . . 701 352 159 LEU CA C 55.155 . . 702 352 159 LEU CB C 41.983 . . 703 352 159 LEU N N 122.192 . . 704 353 160 ILE H H 8.060 . . 705 353 160 ILE C C 175.715 . . 706 353 160 ILE CA C 60.526 . . 707 353 160 ILE CB C 38.452 . . 708 353 160 ILE N N 122.607 . . 709 354 161 ALA H H 8.407 . . 710 354 161 ALA C C 177.664 . . 711 354 161 ALA CA C 52.485 . . 712 354 161 ALA CB C 19.180 . . 713 354 161 ALA N N 128.692 . . 714 355 162 THR H H 8.044 . . 715 355 162 THR C C 174.109 . . 716 355 162 THR CA C 61.667 . . 717 355 162 THR CB C 69.833 . . 718 355 162 THR N N 113.656 . . 719 356 163 ALA H H 8.206 . . 720 356 163 ALA C C 177.283 . . 721 356 163 ALA CA C 52.435 . . 722 356 163 ALA CB C 19.073 . . 723 356 163 ALA N N 125.854 . . 724 357 164 PHE H H 8.181 . . 725 357 164 PHE C C 176.119 . . 726 357 164 PHE CA C 57.620 . . 727 357 164 PHE CB C 39.310 . . 728 357 164 PHE N N 119.311 . . 729 358 165 THR H H 8.049 . . 730 358 165 THR C C 174.084 . . 731 358 165 THR CA C 61.707 . . 732 358 165 THR CB C 69.716 . . 733 358 165 THR N N 115.420 . . 734 359 166 ASN H H 8.335 . . 735 359 166 ASN C C 175.381 . . 736 359 166 ASN CA C 53.328 . . 737 359 166 ASN CB C 38.642 . . 738 359 166 ASN N N 120.646 . . 739 360 167 GLY H H 8.225 . . 740 360 167 GLY C C 173.468 . . 741 360 167 GLY CA C 45.079 . . 742 360 167 GLY N N 108.830 . . 743 361 168 TYR H H 7.983 . . 744 361 168 TYR C C 174.837 . . 745 361 168 TYR CA C 57.961 . . 746 361 168 TYR CB C 38.555 . . 747 361 168 TYR N N 120.314 . . 748 362 169 HIS H H 7.776 . . 749 362 169 HIS C C 178.140 . . 750 362 169 HIS CA C 56.718 . . 751 362 169 HIS CB C 29.873 . . 752 362 169 HIS N N 124.925 . . stop_ save_