data_50128 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; SUMO1 flavonoids ; _BMRB_accession_number 50128 _BMRB_flat_file_name bmr50128.str _Entry_type original _Submission_date 2019-12-14 _Accession_date 2019-12-14 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'HSQC spectrums of human SUMO1 treated with flavonoids' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Velazhahan Vaithish . . 2 Schrick Kathrin . . 3 Prakash Om . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 13 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 1144 "15N chemical shifts" 1144 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-09-19 original BMRB . stop_ _Original_release_date 2019-12-16 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Dietary flavonoid fisetin binds human SUMO1 and blocks sumoylation of p53 ; _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Velazhahan Vaithish . . 2 Glaza Przemyslaw . . 3 Herrera Alvaro I. . 4 Prakash Om . . 5 Zolkiewski Michal . . 6 Geisbrecht Brian V. . 7 Schrick Kathrin . . stop_ _Journal_abbreviation 'Not known' _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly_1 _Saveframe_category molecular_system _Mol_system_name SUMO1 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label SUMO1 $entity_1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 97 _Mol_residue_sequence ; MSDQEAKPSTEDLGDKKEGE YIKLKVIGQDSSEIHFKVKM TTHLKKLKESYCQRQGVPMN SLRFLFEGQRIADNHTPKEL GMEEEDVIEVYQEQTGG ; loop_ _Residue_seq_code _Residue_label 1 MET 2 SER 3 ASP 4 GLN 5 GLU 6 ALA 7 LYS 8 PRO 9 SER 10 THR 11 GLU 12 ASP 13 LEU 14 GLY 15 ASP 16 LYS 17 LYS 18 GLU 19 GLY 20 GLU 21 TYR 22 ILE 23 LYS 24 LEU 25 LYS 26 VAL 27 ILE 28 GLY 29 GLN 30 ASP 31 SER 32 SER 33 GLU 34 ILE 35 HIS 36 PHE 37 LYS 38 VAL 39 LYS 40 MET 41 THR 42 THR 43 HIS 44 LEU 45 LYS 46 LYS 47 LEU 48 LYS 49 GLU 50 SER 51 TYR 52 CYS 53 GLN 54 ARG 55 GLN 56 GLY 57 VAL 58 PRO 59 MET 60 ASN 61 SER 62 LEU 63 ARG 64 PHE 65 LEU 66 PHE 67 GLU 68 GLY 69 GLN 70 ARG 71 ILE 72 ALA 73 ASP 74 ASN 75 HIS 76 THR 77 PRO 78 LYS 79 GLU 80 LEU 81 GLY 82 MET 83 GLU 84 GLU 85 GLU 86 ASP 87 VAL 88 ILE 89 GLU 90 VAL 91 TYR 92 GLN 93 GLU 94 GLN 95 THR 96 GLY 97 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source_1 _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . Escherichia coli 'BL21 Rosetta' plasmid 'Addgene #53138' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated with DMSO as control for 100 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] DMSO 6.56 uL 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated with DMSO as control for 200 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] DMSO 13.26 uL 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated with DMSO as control for 300 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] DMSO 20.09 uL 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated with DMSO as control for 400 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] DMSO 27.04 uL 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated with DMSO as control for 450 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] DMSO 30.59 uL 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated 100 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] Fisetin 100 uM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_7 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated 200 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] Fisetin 200 uM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_8 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated 300 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] Fisetin 300 uM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_9 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated 400 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] Fisetin 400 uM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_10 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated 450 micromolar fisetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM [U-15N] Fisetin 450 uM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_11 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated 200 micromolar quercetin' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 100 uM [U-15N] Quercetin 200 uM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_12 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated 200 micromolar Kaempferol' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 100 uM [U-15N] Kaempferol 200 uM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ save_sample_13 _Saveframe_category sample _Sample_type solution _Details '15N, 1H; SUMO1 treated with DMSO as control for 200 micromolar quercetin or kaempferol' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 100 uM [U-15N] DMSO 6.67 uL 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' DTT 2 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' 'sodium azide' 0.001 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model UnityPlus _Field_strength 500 _Details 'VNMR 500 MHz spectrometer' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_2D_1H-15N_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_3 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_4 save_ save_2D_1H-15N_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_5 save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_6 save_ save_2D_1H-15N_HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_7 save_ save_2D_1H-15N_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_8 save_ save_2D_1H-15N_HSQC_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_9 save_ save_2D_1H-15N_HSQC_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_10 save_ save_2D_1H-15N_HSQC_11 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_13 save_ save_2D_1H-15N_HSQC_12 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_11 save_ save_2D_1H-15N_HSQC_13 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_12 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with DMSO as control for 100 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with DMSO as control for 200 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with DMSO as control for 300 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_4 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with DMSO as control for 400 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_5 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with DMSO as control for 450 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_6 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with 100 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_7 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with 200 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_8 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with 300 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_9 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with 400 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_10 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with 450 micromolar fisetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_11 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Wilmad 535 NMR sample tube. SUMO1 treated with DMSO as control for 200 micromolar quercetin or kaempferol. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_12 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Shigemi NMR tubes. SUMO1 treated with 200 micromolar Quercetin. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ save_sample_conditions_13 _Saveframe_category sample_conditions _Details ; The data was collected in 5 mm Shigemi NMR tubes. SUMO1 treated with 200 micromolar Kaempferol. ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.05 . M pH 6.4 . pH pressure 1 . atm temperature 290 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DMSO-d5 C 13 'methyl protons' ppm 2.5 internal indirect . . . 0.25145 DMSO-d5 H 1 'methyl protons' ppm 2.5 internal direct . . . 1 DMSO-d5 N 15 'methyl protons' ppm 2.2 internal indirect . . . 0.10136 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.307 . . 2 5 5 GLU N N 121.403 . . 3 6 6 ALA H H 8.335 . . 4 6 6 ALA N N 125.8 . . 5 7 7 LYS H H 8.346 . . 6 7 7 LYS N N 122.086 . . 7 9 9 SER H H 8.605 . . 8 9 9 SER N N 117.001 . . 9 10 10 THR H H 8.253 . . 10 10 10 THR N N 115.951 . . 11 11 11 GLU H H 8.365 . . 12 11 11 GLU N N 122.96 . . 13 12 12 ASP H H 8.415 . . 14 12 12 ASP N N 122.22 . . 15 13 13 LEU H H 8.4 . . 16 13 13 LEU N N 123.816 . . 17 14 14 GLY H H 8.457 . . 18 14 14 GLY N N 108.999 . . 19 15 15 ASP H H 8.213 . . 20 15 15 ASP N N 120.501 . . 21 16 16 LYS H H 8.307 . . 22 16 16 LYS N N 121.403 . . 23 17 17 LYS H H 8.415 . . 24 17 17 LYS N N 122.22 . . 25 18 18 GLU H H 8.498 . . 26 18 18 GLU N N 121.979 . . 27 19 19 GLY H H 8.463 . . 28 19 19 GLY N N 109.762 . . 29 20 20 GLU H H 8.22 . . 30 20 20 GLU N N 119.661 . . 31 21 21 TYR H H 8.317 . . 32 21 21 TYR N N 119.301 . . 33 22 22 ILE H H 9.16 . . 34 22 22 ILE N N 116.77 . . 35 23 23 LYS H H 8.614 . . 36 23 23 LYS N N 124.31 . . 37 24 24 LEU H H 8.923 . . 38 24 24 LEU N N 123.99 . . 39 25 25 LYS H H 8.636 . . 40 25 25 LYS N N 120.753 . . 41 26 26 VAL H H 9.1 . . 42 26 26 VAL N N 124.144 . . 43 27 27 ILE H H 9.069 . . 44 27 27 ILE N N 128.361 . . 45 28 28 GLY H H 8.473 . . 46 28 28 GLY N N 113.356 . . 47 29 29 GLN H H 8.771 . . 48 29 29 GLN N N 120.562 . . 49 30 30 ASP H H 8.337 . . 50 30 30 ASP N N 118.415 . . 51 31 31 SER H H 7.999 . . 52 31 31 SER N N 111.152 . . 53 32 32 SER H H 8.085 . . 54 32 32 SER N N 117.561 . . 55 33 33 GLU H H 8.614 . . 56 33 33 GLU N N 121.83 . . 57 34 34 ILE H H 8.669 . . 58 34 34 ILE N N 124.507 . . 59 35 35 HIS H H 8.727 . . 60 35 35 HIS N N 125.533 . . 61 36 36 PHE H H 9.088 . . 62 36 36 PHE N N 120.071 . . 63 37 37 LYS H H 8.79 . . 64 37 37 LYS N N 123.886 . . 65 38 38 VAL H H 9.144 . . 66 38 38 VAL N N 119.773 . . 67 39 39 LYS H H 8.625 . . 68 39 39 LYS N N 122.318 . . 69 40 40 MET H H 8.574 . . 70 40 40 MET N N 120.011 . . 71 42 42 THR H H 7.184 . . 72 42 42 THR N N 120.745 . . 73 43 43 HIS H H 8.853 . . 74 43 43 HIS N N 126.192 . . 75 44 44 LEU H H 9.361 . . 76 44 44 LEU N N 124.984 . . 77 45 45 LYS H H 8.548 . . 78 45 45 LYS N N 121.489 . . 79 46 46 LYS H H 7.511 . . 80 46 46 LYS N N 114.093 . . 81 47 47 LEU H H 6.735 . . 82 47 47 LEU N N 121.951 . . 83 48 48 LYS H H 7.551 . . 84 48 48 LYS N N 118.314 . . 85 49 49 GLU H H 8.503 . . 86 49 49 GLU N N 118.17 . . 87 50 50 SER H H 7.962 . . 88 50 50 SER N N 115.722 . . 89 51 51 TYR H H 8.834 . . 90 51 51 TYR N N 122.729 . . 91 52 52 CYS H H 8.669 . . 92 52 52 CYS N N 117.476 . . 93 53 53 GLN H H 8.328 . . 94 53 53 GLN N N 119.167 . . 95 54 54 ARG H H 8.011 . . 96 54 54 ARG N N 120.241 . . 97 55 55 GLN H H 7.802 . . 98 55 55 GLN N N 114.202 . . 99 56 56 GLY H H 7.926 . . 100 56 56 GLY N N 109.814 . . 101 57 57 VAL H H 7.652 . . 102 57 57 VAL N N 112.041 . . 103 59 59 MET H H 9.007 . . 104 59 59 MET N N 124.575 . . 105 60 60 ASN H H 8.433 . . 106 60 60 ASN N N 113.273 . . 107 61 61 SER H H 7.929 . . 108 61 61 SER N N 113.126 . . 109 62 62 LEU H H 7.262 . . 110 62 62 LEU N N 121.02 . . 111 63 63 ARG H H 9.001 . . 112 63 63 ARG N N 120.266 . . 113 64 64 PHE H H 8.934 . . 114 64 64 PHE N N 122.592 . . 115 65 65 LEU H H 9.738 . . 116 65 65 LEU N N 124.295 . . 117 66 66 PHE H H 8.956 . . 118 66 66 PHE N N 119.446 . . 119 67 67 GLU H H 9.372 . . 120 67 67 GLU N N 129.286 . . 121 68 68 GLY H H 8.868 . . 122 68 68 GLY N N 103.37 . . 123 69 69 GLN H H 7.986 . . 124 69 69 GLN N N 120.747 . . 125 70 70 ARG H H 8.822 . . 126 70 70 ARG N N 125.081 . . 127 71 71 ILE H H 9.033 . . 128 71 71 ILE N N 127 . . 129 73 73 ASP H H 8.771 . . 130 73 73 ASP N N 120.562 . . 131 74 74 ASN H H 7.757 . . 132 74 74 ASN N N 109.624 . . 133 75 75 HIS H H 7.155 . . 134 75 75 HIS N N 119.944 . . 135 76 76 THR H H 7.308 . . 136 76 76 THR N N 108.396 . . 137 78 78 LYS H H 8.081 . . 138 78 78 LYS N N 115.43 . . 139 79 79 GLU H H 7.986 . . 140 79 79 GLU N N 120.747 . . 141 80 80 LEU H H 7.484 . . 142 80 80 LEU N N 115.144 . . 143 81 81 GLY H H 7.646 . . 144 81 81 GLY N N 108.161 . . 145 82 82 MET H H 7.628 . . 146 82 82 MET N N 117.785 . . 147 83 83 GLU H H 9.3 . . 148 83 83 GLU N N 121.082 . . 149 84 84 GLU H H 8.328 . . 150 84 84 GLU N N 119.167 . . 151 85 85 GLU H H 9.362 . . 152 85 85 GLU N N 118.998 . . 153 86 86 ASP H H 8.147 . . 154 86 86 ASP N N 120.282 . . 155 87 87 VAL H H 8.191 . . 156 87 87 VAL N N 116.753 . . 157 88 88 ILE H H 9.155 . . 158 88 88 ILE N N 127.406 . . 159 89 89 GLU H H 8.858 . . 160 89 89 GLU N N 125.905 . . 161 90 90 VAL H H 7.769 . . 162 90 90 VAL N N 119.813 . . 163 91 91 TYR H H 8.534 . . 164 91 91 TYR N N 124.284 . . 165 92 92 GLN H H 8.725 . . 166 92 92 GLN N N 120.853 . . 167 93 93 GLU H H 8.416 . . 168 93 93 GLU N N 125.196 . . 169 94 94 GLN H H 8.787 . . 170 94 94 GLN N N 122.994 . . 171 95 95 THR H H 8.398 . . 172 95 95 THR N N 116.185 . . 173 96 96 GLY H H 8.514 . . 174 96 96 GLY N N 111.935 . . 175 97 97 GLY H H 8.031 . . 176 97 97 GLY N N 115.427 . . stop_ save_ save_assigned_chemical_shifts_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_2 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.307 . . 2 5 5 GLU N N 121.383 . . 3 6 6 ALA H H 8.334 . . 4 6 6 ALA N N 125.7 . . 5 7 7 LYS H H 8.342 . . 6 7 7 LYS N N 122.044 . . 7 9 9 SER H H 8.602 . . 8 9 9 SER N N 116.982 . . 9 10 10 THR H H 8.249 . . 10 10 10 THR N N 115.921 . . 11 11 11 GLU H H 8.363 . . 12 11 11 GLU N N 122.93 . . 13 12 12 ASP H H 8.412 . . 14 12 12 ASP N N 122.201 . . 15 13 13 LEU H H 8.399 . . 16 13 13 LEU N N 123.788 . . 17 14 14 GLY H H 8.457 . . 18 14 14 GLY N N 108.978 . . 19 15 15 ASP H H 8.212 . . 20 15 15 ASP N N 120.485 . . 21 16 16 LYS H H 8.307 . . 22 16 16 LYS N N 121.383 . . 23 17 17 LYS H H 8.412 . . 24 17 17 LYS N N 122.201 . . 25 18 18 GLU H H 8.496 . . 26 18 18 GLU N N 121.954 . . 27 19 19 GLY H H 8.46 . . 28 19 19 GLY N N 109.736 . . 29 20 20 GLU H H 8.22 . . 30 20 20 GLU N N 119.648 . . 31 21 21 TYR H H 8.316 . . 32 21 21 TYR N N 119.288 . . 33 22 22 ILE H H 9.158 . . 34 22 22 ILE N N 116.764 . . 35 23 23 LYS H H 8.616 . . 36 23 23 LYS N N 124.336 . . 37 24 24 LEU H H 8.923 . . 38 24 24 LEU N N 124.039 . . 39 25 25 LYS H H 8.633 . . 40 25 25 LYS N N 120.728 . . 41 26 26 VAL H H 9.104 . . 42 26 26 VAL N N 124.135 . . 43 27 27 ILE H H 9.068 . . 44 27 27 ILE N N 128.378 . . 45 28 28 GLY H H 8.47 . . 46 28 28 GLY N N 113.333 . . 47 29 29 GLN H H 8.768 . . 48 29 29 GLN N N 120.522 . . 49 30 30 ASP H H 8.337 . . 50 30 30 ASP N N 118.396 . . 51 31 31 SER H H 8 . . 52 31 31 SER N N 111.125 . . 53 32 32 SER H H 8.084 . . 54 32 32 SER N N 117.54 . . 55 33 33 GLU H H 8.612 . . 56 33 33 GLU N N 121.816 . . 57 34 34 ILE H H 8.668 . . 58 34 34 ILE N N 124.497 . . 59 35 35 HIS H H 8.726 . . 60 35 35 HIS N N 125.591 . . 61 36 36 PHE H H 9.091 . . 62 36 36 PHE N N 120.052 . . 63 37 37 LYS H H 8.79 . . 64 37 37 LYS N N 123.855 . . 65 38 38 VAL H H 9.14 . . 66 38 38 VAL N N 119.725 . . 67 39 39 LYS H H 8.625 . . 68 39 39 LYS N N 122.306 . . 69 40 40 MET H H 8.572 . . 70 40 40 MET N N 119.998 . . 71 42 42 THR H H 7.183 . . 72 42 42 THR N N 120.745 . . 73 43 43 HIS H H 8.849 . . 74 43 43 HIS N N 126.212 . . 75 44 44 LEU H H 9.358 . . 76 44 44 LEU N N 124.965 . . 77 45 45 LYS H H 8.548 . . 78 45 45 LYS N N 121.49 . . 79 46 46 LYS H H 7.513 . . 80 46 46 LYS N N 114.09 . . 81 47 47 LEU H H 6.737 . . 82 47 47 LEU N N 121.951 . . 83 48 48 LYS H H 7.55 . . 84 48 48 LYS N N 118.326 . . 85 49 49 GLU H H 8.503 . . 86 49 49 GLU N N 118.178 . . 87 50 50 SER H H 7.957 . . 88 50 50 SER N N 115.695 . . 89 51 51 TYR H H 8.835 . . 90 51 51 TYR N N 122.705 . . 91 52 52 CYS H H 8.669 . . 92 52 52 CYS N N 117.465 . . 93 53 53 GLN H H 8.328 . . 94 53 53 GLN N N 119.176 . . 95 54 54 ARG H H 8.013 . . 96 54 54 ARG N N 120.207 . . 97 55 55 GLN H H 7.799 . . 98 55 55 GLN N N 114.175 . . 99 56 56 GLY H H 7.924 . . 100 56 56 GLY N N 109.779 . . 101 57 57 VAL H H 7.653 . . 102 57 57 VAL N N 112.032 . . 103 59 59 MET H H 9.006 . . 104 59 59 MET N N 124.562 . . 105 60 60 ASN H H 8.432 . . 106 60 60 ASN N N 113.268 . . 107 61 61 SER H H 7.928 . . 108 61 61 SER N N 113.102 . . 109 62 62 LEU H H 7.263 . . 110 62 62 LEU N N 121.012 . . 111 63 63 ARG H H 9.002 . . 112 63 63 ARG N N 120.281 . . 113 64 64 PHE H H 8.936 . . 114 64 64 PHE N N 122.606 . . 115 65 65 LEU H H 9.737 . . 116 65 65 LEU N N 124.3 . . 117 66 66 PHE H H 8.959 . . 118 66 66 PHE N N 119.449 . . 119 67 67 GLU H H 9.372 . . 120 67 67 GLU N N 129.264 . . 121 68 68 GLY H H 8.866 . . 122 68 68 GLY N N 103.351 . . 123 69 69 GLN H H 7.986 . . 124 69 69 GLN N N 120.727 . . 125 70 70 ARG H H 8.823 . . 126 70 70 ARG N N 125.086 . . 127 71 71 ILE H H 9.031 . . 128 71 71 ILE N N 126.991 . . 129 73 73 ASP H H 8.768 . . 130 73 73 ASP N N 120.522 . . 131 74 74 ASN H H 7.758 . . 132 74 74 ASN N N 109.62 . . 133 75 75 HIS H H 7.154 . . 134 75 75 HIS N N 119.917 . . 135 76 76 THR H H 7.31 . . 136 76 76 THR N N 108.407 . . 137 78 78 LYS H H 8.082 . . 138 78 78 LYS N N 115.429 . . 139 79 79 GLU H H 7.986 . . 140 79 79 GLU N N 120.727 . . 141 80 80 LEU H H 7.481 . . 142 80 80 LEU N N 115.126 . . 143 81 81 GLY H H 7.646 . . 144 81 81 GLY N N 108.142 . . 145 82 82 MET H H 7.625 . . 146 82 82 MET N N 117.766 . . 147 83 83 GLU H H 9.302 . . 148 83 83 GLU N N 121.084 . . 149 84 84 GLU H H 8.328 . . 150 84 84 GLU N N 119.176 . . 151 85 85 GLU H H 9.364 . . 152 85 85 GLU N N 118.97 . . 153 86 86 ASP H H 8.146 . . 154 86 86 ASP N N 120.307 . . 155 87 87 VAL H H 8.194 . . 156 87 87 VAL N N 116.751 . . 157 88 88 ILE H H 9.153 . . 158 88 88 ILE N N 127.411 . . 159 89 89 GLU H H 8.857 . . 160 89 89 GLU N N 125.914 . . 161 90 90 VAL H H 7.769 . . 162 90 90 VAL N N 119.821 . . 163 91 91 TYR H H 8.531 . . 164 91 91 TYR N N 124.243 . . 165 92 92 GLN H H 8.728 . . 166 92 92 GLN N N 120.836 . . 167 93 93 GLU H H 8.414 . . 168 93 93 GLU N N 125.186 . . 169 94 94 GLN H H 8.787 . . 170 94 94 GLN N N 122.992 . . 171 95 95 THR H H 8.394 . . 172 95 95 THR N N 116.153 . . 173 96 96 GLY H H 8.511 . . 174 96 96 GLY N N 111.909 . . 175 97 97 GLY H H 8.026 . . 176 97 97 GLY N N 115.405 . . stop_ save_ save_assigned_chemical_shifts_3 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_3 stop_ _Sample_conditions_label $sample_conditions_4 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.307 . . 2 5 5 GLU N N 121.366 . . 3 6 6 ALA H H 8.33 . . 4 6 6 ALA N N 125.69 . . 5 7 7 LYS H H 8.338 . . 6 7 7 LYS N N 122.001 . . 7 9 9 SER H H 8.601 . . 8 9 9 SER N N 116.962 . . 9 10 10 THR H H 8.248 . . 10 10 10 THR N N 115.9 . . 11 11 11 GLU H H 8.362 . . 12 11 11 GLU N N 122.927 . . 13 12 12 ASP H H 8.41 . . 14 12 12 ASP N N 122.186 . . 15 13 13 LEU H H 8.397 . . 16 13 13 LEU N N 123.761 . . 17 14 14 GLY H H 8.458 . . 18 14 14 GLY N N 108.96 . . 19 15 15 ASP H H 8.212 . . 20 15 15 ASP N N 120.469 . . 21 16 16 LYS H H 8.307 . . 22 16 16 LYS N N 121.366 . . 23 17 17 LYS H H 8.41 . . 24 17 17 LYS N N 122.186 . . 25 18 18 GLU H H 8.497 . . 26 18 18 GLU N N 121.932 . . 27 19 19 GLY H H 8.461 . . 28 19 19 GLY N N 109.719 . . 29 20 20 GLU H H 8.219 . . 30 20 20 GLU N N 119.641 . . 31 21 21 TYR H H 8.316 . . 32 21 21 TYR N N 119.273 . . 33 22 22 ILE H H 9.157 . . 34 22 22 ILE N N 116.734 . . 35 23 23 LYS H H 8.619 . . 36 23 23 LYS N N 124.356 . . 37 24 24 LEU H H 8.927 . . 38 24 24 LEU N N 124.078 . . 39 25 25 LYS H H 8.633 . . 40 25 25 LYS N N 120.693 . . 41 26 26 VAL H H 9.107 . . 42 26 26 VAL N N 124.138 . . 43 27 27 ILE H H 9.071 . . 44 27 27 ILE N N 128.395 . . 45 28 28 GLY H H 8.469 . . 46 28 28 GLY N N 113.326 . . 47 29 29 GLN H H 8.77 . . 48 29 29 GLN N N 120.517 . . 49 30 30 ASP H H 8.336 . . 50 30 30 ASP N N 118.382 . . 51 31 31 SER H H 8 . . 52 31 31 SER N N 111.105 . . 53 32 32 SER H H 8.084 . . 54 32 32 SER N N 117.518 . . 55 33 33 GLU H H 8.609 . . 56 33 33 GLU N N 121.797 . . 57 34 34 ILE H H 8.668 . . 58 34 34 ILE N N 124.487 . . 59 35 35 HIS H H 8.727 . . 60 35 35 HIS N N 125.659 . . 61 36 36 PHE H H 9.089 . . 62 36 36 PHE N N 120.027 . . 63 37 37 LYS H H 8.791 . . 64 37 37 LYS N N 123.837 . . 65 38 38 VAL H H 9.139 . . 66 38 38 VAL N N 119.686 . . 67 39 39 LYS H H 8.625 . . 68 39 39 LYS N N 122.315 . . 69 40 40 MET H H 8.572 . . 70 40 40 MET N N 119.992 . . 71 42 42 THR H H 7.181 . . 72 42 42 THR N N 120.743 . . 73 43 43 HIS H H 8.848 . . 74 43 43 HIS N N 126.224 . . 75 44 44 LEU H H 9.352 . . 76 44 44 LEU N N 124.949 . . 77 45 45 LYS H H 8.546 . . 78 45 45 LYS N N 121.475 . . 79 46 46 LYS H H 7.512 . . 80 46 46 LYS N N 114.085 . . 81 47 47 LEU H H 6.738 . . 82 47 47 LEU N N 121.95 . . 83 48 48 LYS H H 7.553 . . 84 48 48 LYS N N 118.311 . . 85 49 49 GLU H H 8.503 . . 86 49 49 GLU N N 118.168 . . 87 50 50 SER H H 7.954 . . 88 50 50 SER N N 115.69 . . 89 51 51 TYR H H 8.834 . . 90 51 51 TYR N N 122.684 . . 91 52 52 CYS H H 8.668 . . 92 52 52 CYS N N 117.445 . . 93 53 53 GLN H H 8.324 . . 94 53 53 GLN N N 119.203 . . 95 54 54 ARG H H 8.015 . . 96 54 54 ARG N N 120.178 . . 97 55 55 GLN H H 7.796 . . 98 55 55 GLN N N 114.15 . . 99 56 56 GLY H H 7.922 . . 100 56 56 GLY N N 109.736 . . 101 57 57 VAL H H 7.653 . . 102 57 57 VAL N N 112.01 . . 103 59 59 MET H H 9.008 . . 104 59 59 MET N N 124.552 . . 105 60 60 ASN H H 8.433 . . 106 60 60 ASN N N 113.27 . . 107 61 61 SER H H 7.927 . . 108 61 61 SER N N 113.077 . . 109 62 62 LEU H H 7.263 . . 110 62 62 LEU N N 121.013 . . 111 63 63 ARG H H 9 . . 112 63 63 ARG N N 120.278 . . 113 64 64 PHE H H 8.938 . . 114 64 64 PHE N N 122.621 . . 115 65 65 LEU H H 9.74 . . 116 65 65 LEU N N 124.296 . . 117 66 66 PHE H H 8.959 . . 118 66 66 PHE N N 119.452 . . 119 67 67 GLU H H 9.37 . . 120 67 67 GLU N N 129.268 . . 121 68 68 GLY H H 8.87 . . 122 68 68 GLY N N 103.338 . . 123 69 69 GLN H H 7.986 . . 124 69 69 GLN N N 120.712 . . 125 70 70 ARG H H 8.823 . . 126 70 70 ARG N N 125.08 . . 127 71 71 ILE H H 9.026 . . 128 71 71 ILE N N 126.984 . . 129 73 73 ASP H H 8.77 . . 130 73 73 ASP N N 120.517 . . 131 74 74 ASN H H 7.755 . . 132 74 74 ASN N N 109.613 . . 133 75 75 HIS H H 7.153 . . 134 75 75 HIS N N 119.911 . . 135 76 76 THR H H 7.311 . . 136 76 76 THR N N 108.41 . . 137 78 78 LYS H H 8.082 . . 138 78 78 LYS N N 115.426 . . 139 79 79 GLU H H 7.986 . . 140 79 79 GLU N N 120.712 . . 141 80 80 LEU H H 7.478 . . 142 80 80 LEU N N 115.113 . . 143 81 81 GLY H H 7.645 . . 144 81 81 GLY N N 108.139 . . 145 82 82 MET H H 7.623 . . 146 82 82 MET N N 117.763 . . 147 83 83 GLU H H 9.305 . . 148 83 83 GLU N N 121.068 . . 149 84 84 GLU H H 8.324 . . 150 84 84 GLU N N 119.203 . . 151 85 85 GLU H H 9.361 . . 152 85 85 GLU N N 118.943 . . 153 86 86 ASP H H 8.145 . . 154 86 86 ASP N N 120.282 . . 155 87 87 VAL H H 8.194 . . 156 87 87 VAL N N 116.748 . . 157 88 88 ILE H H 9.156 . . 158 88 88 ILE N N 127.385 . . 159 89 89 GLU H H 8.858 . . 160 89 89 GLU N N 125.92 . . 161 90 90 VAL H H 7.77 . . 162 90 90 VAL N N 119.812 . . 163 91 91 TYR H H 8.528 . . 164 91 91 TYR N N 124.212 . . 165 92 92 GLN H H 8.73 . . 166 92 92 GLN N N 120.839 . . 167 93 93 GLU H H 8.411 . . 168 93 93 GLU N N 125.182 . . 169 94 94 GLN H H 8.79 . . 170 94 94 GLN N N 122.982 . . 171 95 95 THR H H 8.393 . . 172 95 95 THR N N 116.13 . . 173 96 96 GLY H H 8.511 . . 174 96 96 GLY N N 111.888 . . 175 97 97 GLY H H 8.025 . . 176 97 97 GLY N N 115.386 . . stop_ save_ save_assigned_chemical_shifts_4 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_4 stop_ _Sample_conditions_label $sample_conditions_5 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.117 . . 2 5 5 GLU N N 120.848 . . 3 6 6 ALA H H 8.136 . . 4 6 6 ALA N N 125.157 . . 5 7 7 LYS H H 8.144 . . 6 7 7 LYS N N 121.456 . . 7 9 9 SER H H 8.409 . . 8 9 9 SER N N 116.442 . . 9 10 10 THR H H 8.055 . . 10 10 10 THR N N 115.372 . . 11 11 11 GLU H H 8.172 . . 12 11 11 GLU N N 122.395 . . 13 12 12 ASP H H 8.217 . . 14 12 12 ASP N N 121.667 . . 15 13 13 LEU H H 8.206 . . 16 13 13 LEU N N 123.236 . . 17 14 14 GLY H H 8.269 . . 18 14 14 GLY N N 108.437 . . 19 15 15 ASP H H 8.02 . . 20 15 15 ASP N N 119.955 . . 21 16 16 LYS H H 8.117 . . 22 16 16 LYS N N 120.848 . . 23 17 17 LYS H H 8.217 . . 24 17 17 LYS N N 121.667 . . 25 18 18 GLU H H 8.306 . . 26 18 18 GLU N N 121.399 . . 27 19 19 GLY H H 8.269 . . 28 19 19 GLY N N 109.196 . . 29 20 20 GLU H H 8.029 . . 30 20 20 GLU N N 119.121 . . 31 21 21 TYR H H 8.127 . . 32 21 21 TYR N N 118.775 . . 33 22 22 ILE H H 8.965 . . 34 22 22 ILE N N 116.212 . . 35 23 23 LYS H H 8.435 . . 36 23 23 LYS N N 123.886 . . 37 24 24 LEU H H 8.74 . . 38 24 24 LEU N N 123.632 . . 39 25 25 LYS H H 8.442 . . 40 25 25 LYS N N 120.165 . . 41 26 26 VAL H H 8.918 . . 42 26 26 VAL N N 123.617 . . 43 27 27 ILE H H 8.886 . . 44 27 27 ILE N N 127.925 . . 45 28 28 GLY H H 8.244 . . 46 28 28 GLY N N 112.762 . . 47 29 29 GLN H H 8.576 . . 48 29 29 GLN N N 119.98 . . 49 30 30 ASP H H 8.146 . . 50 30 30 ASP N N 117.871 . . 51 31 31 SER H H 7.811 . . 52 31 31 SER N N 110.582 . . 53 32 32 SER H H 7.893 . . 54 32 32 SER N N 116.99 . . 55 33 33 GLU H H 8.415 . . 56 33 33 GLU N N 121.295 . . 57 34 34 ILE H H 8.477 . . 58 34 34 ILE N N 123.958 . . 59 35 35 HIS H H 8.537 . . 60 35 35 HIS N N 125.23 . . 61 36 36 PHE H H 8.9 . . 62 36 36 PHE N N 119.515 . . 63 37 37 LYS H H 8.602 . . 64 37 37 LYS N N 123.305 . . 65 38 38 VAL H H 8.943 . . 66 38 38 VAL N N 119.127 . . 67 39 39 LYS H H 8.435 . . 68 39 39 LYS N N 121.804 . . 69 40 40 MET H H 8.383 . . 70 40 40 MET N N 119.466 . . 71 42 42 THR H H 6.989 . . 72 42 42 THR N N 120.243 . . 73 43 43 HIS H H 8.657 . . 74 43 43 HIS N N 125.725 . . 75 44 44 LEU H H 9.16 . . 76 44 44 LEU N N 124.439 . . 77 45 45 LYS H H 8.358 . . 78 45 45 LYS N N 120.986 . . 79 46 46 LYS H H 7.324 . . 80 46 46 LYS N N 113.583 . . 81 47 47 LEU H H 6.549 . . 82 47 47 LEU N N 121.469 . . 83 48 48 LYS H H 7.362 . . 84 48 48 LYS N N 117.842 . . 85 49 49 GLU H H 8.313 . . 86 49 49 GLU N N 117.679 . . 87 50 50 SER H H 7.762 . . 88 50 50 SER N N 115.171 . . 89 51 51 TYR H H 8.643 . . 90 51 51 TYR N N 122.153 . . 91 52 52 CYS H H 8.478 . . 92 52 52 CYS N N 116.943 . . 93 53 53 GLN H H 8.127 . . 94 53 53 GLN N N 118.775 . . 95 54 54 ARG H H 7.825 . . 96 54 54 ARG N N 119.648 . . 97 55 55 GLN H H 7.605 . . 98 55 55 GLN N N 113.641 . . 99 56 56 GLY H H 7.73 . . 100 56 56 GLY N N 109.204 . . 101 57 57 VAL H H 7.463 . . 102 57 57 VAL N N 111.501 . . 103 59 59 MET H H 8.817 . . 104 59 59 MET N N 124.048 . . 105 60 60 ASN H H 8.244 . . 106 60 60 ASN N N 112.762 . . 107 61 61 SER H H 7.735 . . 108 61 61 SER N N 112.559 . . 109 62 62 LEU H H 7.074 . . 110 62 62 LEU N N 120.503 . . 111 63 63 ARG H H 8.812 . . 112 63 63 ARG N N 119.8 . . 113 64 64 PHE H H 8.747 . . 114 64 64 PHE N N 122.137 . . 115 65 65 LEU H H 9.548 . . 116 65 65 LEU N N 123.773 . . 117 66 66 PHE H H 8.767 . . 118 66 66 PHE N N 118.949 . . 119 67 67 GLU H H 9.18 . . 120 67 67 GLU N N 128.76 . . 121 68 68 GLY H H 8.679 . . 122 68 68 GLY N N 102.825 . . 123 69 69 GLN H H 7.793 . . 124 69 69 GLN N N 120.184 . . 125 70 70 ARG H H 8.633 . . 126 70 70 ARG N N 124.582 . . 127 71 71 ILE H H 8.836 . . 128 71 71 ILE N N 126.454 . . 129 73 73 ASP H H 8.576 . . 130 73 73 ASP N N 119.98 . . 131 74 74 ASN H H 7.567 . . 132 74 74 ASN N N 109.101 . . 133 75 75 HIS H H 6.962 . . 134 75 75 HIS N N 119.394 . . 135 76 76 THR H H 7.122 . . 136 76 76 THR N N 107.909 . . 137 78 78 LYS H H 7.892 . . 138 78 78 LYS N N 114.926 . . 139 79 79 GLU H H 7.793 . . 140 79 79 GLU N N 120.184 . . 141 80 80 LEU H H 7.285 . . 142 80 80 LEU N N 114.579 . . 143 81 81 GLY H H 7.456 . . 144 81 81 GLY N N 107.617 . . 145 82 82 MET H H 7.432 . . 146 82 82 MET N N 117.268 . . 147 83 83 GLU H H 9.116 . . 148 83 83 GLU N N 120.566 . . 149 84 84 GLU H H 8.127 . . 150 84 84 GLU N N 118.775 . . 151 85 85 GLU H H 9.169 . . 152 85 85 GLU N N 118.449 . . 153 86 86 ASP H H 7.956 . . 154 86 86 ASP N N 119.787 . . 155 87 87 VAL H H 8.005 . . 156 87 87 VAL N N 116.238 . . 157 88 88 ILE H H 8.966 . . 158 88 88 ILE N N 126.888 . . 159 89 89 GLU H H 8.672 . . 160 89 89 GLU N N 125.412 . . 161 90 90 VAL H H 7.58 . . 162 90 90 VAL N N 119.321 . . 163 91 91 TYR H H 8.335 . . 164 91 91 TYR N N 123.693 . . 165 92 92 GLN H H 8.541 . . 166 92 92 GLN N N 120.332 . . 167 93 93 GLU H H 8.22 . . 168 93 93 GLU N N 124.673 . . 169 94 94 GLN H H 8.602 . . 170 94 94 GLN N N 122.474 . . 171 95 95 THR H H 8.2 . . 172 95 95 THR N N 115.6 . . 173 96 96 GLY H H 8.32 . . 174 96 96 GLY N N 111.363 . . 175 97 97 GLY H H 7.832 . . 176 97 97 GLY N N 114.862 . . stop_ save_ save_assigned_chemical_shifts_5 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_5 stop_ _Sample_conditions_label $sample_conditions_6 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.309 . . 2 5 5 GLU N N 121.336 . . 3 6 6 ALA H H 8.329 . . 4 6 6 ALA N N 125.6 . . 5 7 7 LYS H H 8.338 . . 6 7 7 LYS N N 122.294 . . 7 9 9 SER H H 8.6 . . 8 9 9 SER N N 116.932 . . 9 10 10 THR H H 8.245 . . 10 10 10 THR N N 115.863 . . 11 11 11 GLU H H 8.363 . . 12 11 11 GLU N N 123.05 . . 13 12 12 ASP H H 8.408 . . 14 12 12 ASP N N 122.158 . . 15 13 13 LEU H H 8.397 . . 16 13 13 LEU N N 123.72 . . 17 14 14 GLY H H 8.461 . . 18 14 14 GLY N N 108.929 . . 19 15 15 ASP H H 8.211 . . 20 15 15 ASP N N 120.446 . . 21 16 16 LYS H H 8.309 . . 22 16 16 LYS N N 121.336 . . 23 17 17 LYS H H 8.408 . . 24 17 17 LYS N N 122.158 . . 25 18 18 GLU H H 8.496 . . 26 18 18 GLU N N 121.891 . . 27 19 19 GLY H H 8.46 . . 28 19 19 GLY N N 109.686 . . 29 20 20 GLU H H 8.221 . . 30 20 20 GLU N N 119.63 . . 31 21 21 TYR H H 8.32 . . 32 21 21 TYR N N 119.257 . . 33 22 22 ILE H H 9.153 . . 34 22 22 ILE N N 116.681 . . 35 23 23 LYS H H 8.635 . . 36 23 23 LYS N N 124.398 . . 37 24 24 LEU H H 8.933 . . 38 24 24 LEU N N 124.157 . . 39 25 25 LYS H H 8.633 . . 40 25 25 LYS N N 120.648 . . 41 26 26 VAL H H 9.116 . . 42 26 26 VAL N N 124.12 . . 43 27 27 ILE H H 9.077 . . 44 27 27 ILE N N 128.42 . . 45 28 28 GLY H H 8.435 . . 46 28 28 GLY N N 113.259 . . 47 29 29 GLN H H 8.768 . . 48 29 29 GLN N N 120.464 . . 49 30 30 ASP H H 8.338 . . 50 30 30 ASP N N 118.355 . . 51 31 31 SER H H 8.002 . . 52 31 31 SER N N 111.073 . . 53 32 32 SER H H 8.086 . . 54 32 32 SER N N 117.486 . . 55 33 33 GLU H H 8.605 . . 56 33 33 GLU N N 121.782 . . 57 34 34 ILE H H 8.667 . . 58 34 34 ILE N N 124.45 . . 59 35 35 HIS H H 8.731 . . 60 35 35 HIS N N 125.749 . . 61 36 36 PHE H H 9.093 . . 62 36 36 PHE N N 119.995 . . 63 37 37 LYS H H 8.794 . . 64 37 37 LYS N N 123.786 . . 65 38 38 VAL H H 9.135 . . 66 38 38 VAL N N 119.62 . . 67 39 39 LYS H H 8.626 . . 68 39 39 LYS N N 122.333 . . 69 40 40 MET H H 8.574 . . 70 40 40 MET N N 119.95 . . 71 42 42 THR H H 7.183 . . 72 42 42 THR N N 120.726 . . 73 43 43 HIS H H 8.845 . . 74 43 43 HIS N N 126.228 . . 75 44 44 LEU H H 9.355 . . 76 44 44 LEU N N 124.922 . . 77 45 45 LYS H H 8.547 . . 78 45 45 LYS N N 121.472 . . 79 46 46 LYS H H 7.518 . . 80 46 46 LYS N N 114.086 . . 81 47 47 LEU H H 6.744 . . 82 47 47 LEU N N 121.932 . . 83 48 48 LYS H H 7.556 . . 84 48 48 LYS N N 118.329 . . 85 49 49 GLU H H 8.506 . . 86 49 49 GLU N N 118.171 . . 87 50 50 SER H H 7.951 . . 88 50 50 SER N N 115.657 . . 89 51 51 TYR H H 8.836 . . 90 51 51 TYR N N 122.655 . . 91 52 52 CYS H H 8.668 . . 92 52 52 CYS N N 117.437 . . 93 53 53 GLN H H 8.32 . . 94 53 53 GLN N N 119.257 . . 95 54 54 ARG H H 8.016 . . 96 54 54 ARG N N 120.132 . . 97 55 55 GLN H H 7.794 . . 98 55 55 GLN N N 114.119 . . 99 56 56 GLY H H 7.922 . . 100 56 56 GLY N N 109.693 . . 101 57 57 VAL H H 7.654 . . 102 57 57 VAL N N 111.995 . . 103 59 59 MET H H 9.009 . . 104 59 59 MET N N 124.54 . . 105 60 60 ASN H H 8.435 . . 106 60 60 ASN N N 113.259 . . 107 61 61 SER H H 7.927 . . 108 61 61 SER N N 113.042 . . 109 62 62 LEU H H 7.268 . . 110 62 62 LEU N N 121.006 . . 111 63 63 ARG H H 9.003 . . 112 63 63 ARG N N 120.299 . . 113 64 64 PHE H H 8.939 . . 114 64 64 PHE N N 122.646 . . 115 65 65 LEU H H 9.739 . . 116 65 65 LEU N N 124.296 . . 117 66 66 PHE H H 8.966 . . 118 66 66 PHE N N 119.462 . . 119 67 67 GLU H H 9.377 . . 120 67 67 GLU N N 129.267 . . 121 68 68 GLY H H 8.867 . . 122 68 68 GLY N N 103.311 . . 123 69 69 GLN H H 7.99 . . 124 69 69 GLN N N 120.684 . . 125 70 70 ARG H H 8.826 . . 126 70 70 ARG N N 125.098 . . 127 71 71 ILE H H 9.023 . . 128 71 71 ILE N N 126.951 . . 129 73 73 ASP H H 8.768 . . 130 73 73 ASP N N 120.464 . . 131 74 74 ASN H H 7.757 . . 132 74 74 ASN N N 109.6 . . 133 75 75 HIS H H 7.154 . . 134 75 75 HIS N N 119.903 . . 135 76 76 THR H H 7.316 . . 136 76 76 THR N N 108.407 . . 137 78 78 LYS H H 8.023 . . 138 78 78 LYS N N 115.354 . . 139 79 79 GLU H H 7.96 . . 140 79 79 GLU N N 120.844 . . 141 80 80 LEU H H 7.476 . . 142 80 80 LEU N N 115.072 . . 143 81 81 GLY H H 7.649 . . 144 81 81 GLY N N 108.117 . . 145 82 82 MET H H 7.632 . . 146 82 82 MET N N 117.797 . . 147 83 83 GLU H H 9.312 . . 148 83 83 GLU N N 121.036 . . 149 84 84 GLU H H 8.32 . . 150 84 84 GLU N N 119.257 . . 151 85 85 GLU H H 9.357 . . 152 85 85 GLU N N 118.935 . . 153 86 86 ASP H H 8.148 . . 154 86 86 ASP N N 120.265 . . 155 87 87 VAL H H 8.195 . . 156 87 87 VAL N N 116.737 . . 157 88 88 ILE H H 9.161 . . 158 88 88 ILE N N 127.407 . . 159 89 89 GLU H H 8.862 . . 160 89 89 GLU N N 125.916 . . 161 90 90 VAL H H 7.774 . . 162 90 90 VAL N N 119.816 . . 163 91 91 TYR H H 8.528 . . 164 91 91 TYR N N 124.158 . . 165 92 92 GLN H H 8.737 . . 166 92 92 GLN N N 120.841 . . 167 93 93 GLU H H 8.412 . . 168 93 93 GLU N N 125.176 . . 169 94 94 GLN H H 8.794 . . 170 94 94 GLN N N 122.969 . . 171 95 95 THR H H 8.39 . . 172 95 95 THR N N 116.087 . . 173 96 96 GLY H H 8.511 . . 174 96 96 GLY N N 111.853 . . 175 97 97 GLY H H 8.023 . . 176 97 97 GLY N N 115.354 . . stop_ save_ save_assigned_chemical_shifts_6 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_6 stop_ _Sample_conditions_label $sample_conditions_7 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.307 . . 2 5 5 GLU N N 121.403 . . 3 6 6 ALA H H 8.335 . . 4 6 6 ALA N N 125.8 . . 5 7 7 LYS H H 8.346 . . 6 7 7 LYS N N 122.086 . . 7 9 9 SER H H 8.605 . . 8 9 9 SER N N 117.001 . . 9 10 10 THR H H 8.253 . . 10 10 10 THR N N 115.951 . . 11 11 11 GLU H H 8.365 . . 12 11 11 GLU N N 122.96 . . 13 12 12 ASP H H 8.415 . . 14 12 12 ASP N N 122.22 . . 15 13 13 LEU H H 8.4 . . 16 13 13 LEU N N 123.816 . . 17 14 14 GLY H H 8.457 . . 18 14 14 GLY N N 108.999 . . 19 15 15 ASP H H 8.213 . . 20 15 15 ASP N N 120.501 . . 21 16 16 LYS H H 8.307 . . 22 16 16 LYS N N 121.403 . . 23 17 17 LYS H H 8.415 . . 24 17 17 LYS N N 122.22 . . 25 18 18 GLU H H 8.498 . . 26 18 18 GLU N N 121.979 . . 27 19 19 GLY H H 8.463 . . 28 19 19 GLY N N 109.762 . . 29 20 20 GLU H H 8.22 . . 30 20 20 GLU N N 119.661 . . 31 21 21 TYR H H 8.317 . . 32 21 21 TYR N N 119.301 . . 33 22 22 ILE H H 9.16 . . 34 22 22 ILE N N 116.77 . . 35 23 23 LYS H H 8.614 . . 36 23 23 LYS N N 124.31 . . 37 24 24 LEU H H 8.923 . . 38 24 24 LEU N N 123.99 . . 39 25 25 LYS H H 8.636 . . 40 25 25 LYS N N 120.753 . . 41 26 26 VAL H H 9.1 . . 42 26 26 VAL N N 124.144 . . 43 27 27 ILE H H 9.069 . . 44 27 27 ILE N N 128.361 . . 45 28 28 GLY H H 8.473 . . 46 28 28 GLY N N 113.356 . . 47 29 29 GLN H H 8.771 . . 48 29 29 GLN N N 120.562 . . 49 30 30 ASP H H 8.337 . . 50 30 30 ASP N N 118.415 . . 51 31 31 SER H H 7.999 . . 52 31 31 SER N N 111.152 . . 53 32 32 SER H H 8.085 . . 54 32 32 SER N N 117.561 . . 55 33 33 GLU H H 8.614 . . 56 33 33 GLU N N 121.83 . . 57 34 34 ILE H H 8.669 . . 58 34 34 ILE N N 124.507 . . 59 35 35 HIS H H 8.727 . . 60 35 35 HIS N N 125.533 . . 61 36 36 PHE H H 9.088 . . 62 36 36 PHE N N 120.071 . . 63 37 37 LYS H H 8.79 . . 64 37 37 LYS N N 123.886 . . 65 38 38 VAL H H 9.144 . . 66 38 38 VAL N N 119.773 . . 67 39 39 LYS H H 8.625 . . 68 39 39 LYS N N 122.318 . . 69 40 40 MET H H 8.574 . . 70 40 40 MET N N 120.011 . . 71 42 42 THR H H 7.184 . . 72 42 42 THR N N 120.745 . . 73 43 43 HIS H H 8.853 . . 74 43 43 HIS N N 126.192 . . 75 44 44 LEU H H 9.361 . . 76 44 44 LEU N N 124.984 . . 77 45 45 LYS H H 8.548 . . 78 45 45 LYS N N 121.489 . . 79 46 46 LYS H H 7.511 . . 80 46 46 LYS N N 114.093 . . 81 47 47 LEU H H 6.735 . . 82 47 47 LEU N N 121.951 . . 83 48 48 LYS H H 7.551 . . 84 48 48 LYS N N 118.314 . . 85 49 49 GLU H H 8.503 . . 86 49 49 GLU N N 118.17 . . 87 50 50 SER H H 7.962 . . 88 50 50 SER N N 115.722 . . 89 51 51 TYR H H 8.834 . . 90 51 51 TYR N N 122.729 . . 91 52 52 CYS H H 8.669 . . 92 52 52 CYS N N 117.476 . . 93 53 53 GLN H H 8.328 . . 94 53 53 GLN N N 119.167 . . 95 54 54 ARG H H 8.011 . . 96 54 54 ARG N N 120.241 . . 97 55 55 GLN H H 7.802 . . 98 55 55 GLN N N 114.202 . . 99 56 56 GLY H H 7.926 . . 100 56 56 GLY N N 109.814 . . 101 57 57 VAL H H 7.652 . . 102 57 57 VAL N N 112.041 . . 103 59 59 MET H H 9.007 . . 104 59 59 MET N N 124.575 . . 105 60 60 ASN H H 8.433 . . 106 60 60 ASN N N 113.273 . . 107 61 61 SER H H 7.929 . . 108 61 61 SER N N 113.126 . . 109 62 62 LEU H H 7.262 . . 110 62 62 LEU N N 121.02 . . 111 63 63 ARG H H 9.001 . . 112 63 63 ARG N N 120.266 . . 113 64 64 PHE H H 8.934 . . 114 64 64 PHE N N 122.592 . . 115 65 65 LEU H H 9.738 . . 116 65 65 LEU N N 124.295 . . 117 66 66 PHE H H 8.956 . . 118 66 66 PHE N N 119.446 . . 119 67 67 GLU H H 9.372 . . 120 67 67 GLU N N 129.286 . . 121 68 68 GLY H H 8.868 . . 122 68 68 GLY N N 103.37 . . 123 69 69 GLN H H 7.986 . . 124 69 69 GLN N N 120.747 . . 125 70 70 ARG H H 8.822 . . 126 70 70 ARG N N 125.081 . . 127 71 71 ILE H H 9.033 . . 128 71 71 ILE N N 127 . . 129 73 73 ASP H H 8.771 . . 130 73 73 ASP N N 120.562 . . 131 74 74 ASN H H 7.757 . . 132 74 74 ASN N N 109.624 . . 133 75 75 HIS H H 7.155 . . 134 75 75 HIS N N 119.944 . . 135 76 76 THR H H 7.308 . . 136 76 76 THR N N 108.396 . . 137 78 78 LYS H H 8.081 . . 138 78 78 LYS N N 115.43 . . 139 79 79 GLU H H 7.986 . . 140 79 79 GLU N N 120.747 . . 141 80 80 LEU H H 7.484 . . 142 80 80 LEU N N 115.144 . . 143 81 81 GLY H H 7.646 . . 144 81 81 GLY N N 108.161 . . 145 82 82 MET H H 7.628 . . 146 82 82 MET N N 117.785 . . 147 83 83 GLU H H 9.3 . . 148 83 83 GLU N N 121.082 . . 149 84 84 GLU H H 8.328 . . 150 84 84 GLU N N 119.167 . . 151 85 85 GLU H H 9.362 . . 152 85 85 GLU N N 118.998 . . 153 86 86 ASP H H 8.147 . . 154 86 86 ASP N N 120.282 . . 155 87 87 VAL H H 8.191 . . 156 87 87 VAL N N 116.753 . . 157 88 88 ILE H H 9.155 . . 158 88 88 ILE N N 127.406 . . 159 89 89 GLU H H 8.858 . . 160 89 89 GLU N N 125.905 . . 161 90 90 VAL H H 7.769 . . 162 90 90 VAL N N 119.813 . . 163 91 91 TYR H H 8.534 . . 164 91 91 TYR N N 124.284 . . 165 92 92 GLN H H 8.725 . . 166 92 92 GLN N N 120.853 . . 167 93 93 GLU H H 8.416 . . 168 93 93 GLU N N 125.196 . . 169 94 94 GLN H H 8.787 . . 170 94 94 GLN N N 122.994 . . 171 95 95 THR H H 8.398 . . 172 95 95 THR N N 116.185 . . 173 96 96 GLY H H 8.514 . . 174 96 96 GLY N N 111.935 . . 175 97 97 GLY H H 8.031 . . 176 97 97 GLY N N 115.427 . . stop_ save_ save_assigned_chemical_shifts_7 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_7 stop_ _Sample_conditions_label $sample_conditions_8 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.304 . . 2 5 5 GLU N N 121.39 . . 3 6 6 ALA H H 8.33 . . 4 6 6 ALA N N 125.7 . . 5 7 7 LYS H H 8.34 . . 6 7 7 LYS N N 122.058 . . 7 9 9 SER H H 8.6 . . 8 9 9 SER N N 116.993 . . 9 10 10 THR H H 8.247 . . 10 10 10 THR N N 115.934 . . 11 11 11 GLU H H 8.36 . . 12 11 11 GLU N N 122.944 . . 13 12 12 ASP H H 8.41 . . 14 12 12 ASP N N 122.211 . . 15 13 13 LEU H H 8.395 . . 16 13 13 LEU N N 123.798 . . 17 14 14 GLY H H 8.455 . . 18 14 14 GLY N N 108.984 . . 19 15 15 ASP H H 8.209 . . 20 15 15 ASP N N 120.494 . . 21 16 16 LYS H H 8.304 . . 22 16 16 LYS N N 121.39 . . 23 17 17 LYS H H 8.41 . . 24 17 17 LYS N N 122.211 . . 25 18 18 GLU H H 8.494 . . 26 18 18 GLU N N 121.962 . . 27 19 19 GLY H H 8.458 . . 28 19 19 GLY N N 109.75 . . 29 20 20 GLU H H 8.217 . . 30 20 20 GLU N N 119.658 . . 31 21 21 TYR H H 8.313 . . 32 21 21 TYR N N 119.296 . . 33 22 22 ILE H H 9.154 . . 34 22 22 ILE N N 116.745 . . 35 23 23 LYS H H 8.613 . . 36 23 23 LYS N N 124.34 . . 37 24 24 LEU H H 8.922 . . 38 24 24 LEU N N 124.053 . . 39 25 25 LYS H H 8.631 . . 40 25 25 LYS N N 120.735 . . 41 26 26 VAL H H 9.1 . . 42 26 26 VAL N N 124.138 . . 43 27 27 ILE H H 9.067 . . 44 27 27 ILE N N 128.383 . . 45 28 28 GLY H H 8.472 . . 46 28 28 GLY N N 113.353 . . 47 29 29 GLN H H 8.763 . . 48 29 29 GLN N N 120.537 . . 49 30 30 ASP H H 8.334 . . 50 30 30 ASP N N 118.41 . . 51 31 31 SER H H 7.998 . . 52 31 31 SER N N 111.146 . . 53 32 32 SER H H 8.081 . . 54 32 32 SER N N 117.552 . . 55 33 33 GLU H H 8.608 . . 56 33 33 GLU N N 121.829 . . 57 34 34 ILE H H 8.664 . . 58 34 34 ILE N N 124.497 . . 59 35 35 HIS H H 8.722 . . 60 35 35 HIS N N 125.594 . . 61 36 36 PHE H H 9.087 . . 62 36 36 PHE N N 120.064 . . 63 37 37 LYS H H 8.787 . . 64 37 37 LYS N N 123.846 . . 65 38 38 VAL H H 9.138 . . 66 38 38 VAL N N 119.728 . . 67 39 39 LYS H H 8.62 . . 68 39 39 LYS N N 122.32 . . 69 40 40 MET H H 8.571 . . 70 40 40 MET N N 120.009 . . 71 42 42 THR H H 7.178 . . 72 42 42 THR N N 120.759 . . 73 43 43 HIS H H 8.846 . . 74 43 43 HIS N N 126.217 . . 75 44 44 LEU H H 9.353 . . 76 44 44 LEU N N 124.962 . . 77 45 45 LYS H H 8.544 . . 78 45 45 LYS N N 121.506 . . 79 46 46 LYS H H 7.511 . . 80 46 46 LYS N N 114.105 . . 81 47 47 LEU H H 6.736 . . 82 47 47 LEU N N 121.964 . . 83 48 48 LYS H H 7.546 . . 84 48 48 LYS N N 118.342 . . 85 49 49 GLU H H 8.501 . . 86 49 49 GLU N N 118.184 . . 87 50 50 SER H H 7.954 . . 88 50 50 SER N N 115.706 . . 89 51 51 TYR H H 8.831 . . 90 51 51 TYR N N 122.711 . . 91 52 52 CYS H H 8.665 . . 92 52 52 CYS N N 117.463 . . 93 53 53 GLN H H 8.322 . . 94 53 53 GLN N N 119.184 . . 95 54 54 ARG H H 8.011 . . 96 54 54 ARG N N 120.213 . . 97 55 55 GLN H H 7.797 . . 98 55 55 GLN N N 114.196 . . 99 56 56 GLY H H 7.921 . . 100 56 56 GLY N N 109.796 . . 101 57 57 VAL H H 7.649 . . 102 57 57 VAL N N 112.048 . . 103 59 59 MET H H 9.001 . . 104 59 59 MET N N 124.563 . . 105 60 60 ASN H H 8.428 . . 106 60 60 ASN N N 113.286 . . 107 61 61 SER H H 7.925 . . 108 61 61 SER N N 113.12 . . 109 62 62 LEU H H 7.262 . . 110 62 62 LEU N N 121.017 . . 111 63 63 ARG H H 8.998 . . 112 63 63 ARG N N 120.293 . . 113 64 64 PHE H H 8.932 . . 114 64 64 PHE N N 122.619 . . 115 65 65 LEU H H 9.736 . . 116 65 65 LEU N N 124.317 . . 117 66 66 PHE H H 8.961 . . 118 66 66 PHE N N 119.461 . . 119 67 67 GLU H H 9.373 . . 120 67 67 GLU N N 129.288 . . 121 68 68 GLY H H 8.861 . . 122 68 68 GLY N N 103.347 . . 123 69 69 GLN H H 7.985 . . 124 69 69 GLN N N 120.744 . . 125 70 70 ARG H H 8.82 . . 126 70 70 ARG N N 125.106 . . 127 71 71 ILE H H 9.025 . . 128 71 71 ILE N N 126.992 . . 129 73 73 ASP H H 8.763 . . 130 73 73 ASP N N 120.537 . . 131 74 74 ASN H H 7.754 . . 132 74 74 ASN N N 109.639 . . 133 75 75 HIS H H 7.152 . . 134 75 75 HIS N N 119.932 . . 135 76 76 THR H H 7.306 . . 136 76 76 THR N N 108.414 . . 137 78 78 LYS H H 8.08 . . 138 78 78 LYS N N 115.444 . . 139 79 79 GLU H H 7.985 . . 140 79 79 GLU N N 120.744 . . 141 80 80 LEU H H 7.477 . . 142 80 80 LEU N N 115.125 . . 143 81 81 GLY H H 7.644 . . 144 81 81 GLY N N 108.173 . . 145 82 82 MET H H 7.628 . . 146 82 82 MET N N 117.793 . . 147 83 83 GLU H H 9.295 . . 148 83 83 GLU N N 121.077 . . 149 84 84 GLU H H 8.322 . . 150 84 84 GLU N N 119.184 . . 151 85 85 GLU H H 9.362 . . 152 85 85 GLU N N 119.002 . . 153 86 86 ASP H H 8.143 . . 154 86 86 ASP N N 120.299 . . 155 87 87 VAL H H 8.187 . . 156 87 87 VAL N N 116.751 . . 157 88 88 ILE H H 9.151 . . 158 88 88 ILE N N 127.417 . . 159 89 89 GLU H H 8.855 . . 160 89 89 GLU N N 125.926 . . 161 90 90 VAL H H 7.766 . . 162 90 90 VAL N N 119.833 . . 163 91 91 TYR H H 8.527 . . 164 91 91 TYR N N 124.26 . . 165 92 92 GLN H H 8.725 . . 166 92 92 GLN N N 120.856 . . 167 93 93 GLU H H 8.412 . . 168 93 93 GLU N N 125.201 . . 169 94 94 GLN H H 8.785 . . 170 94 94 GLN N N 122.999 . . 171 95 95 THR H H 8.392 . . 172 95 95 THR N N 116.167 . . 173 96 96 GLY H H 8.508 . . 174 96 96 GLY N N 111.923 . . 175 97 97 GLY H H 8.026 . . 176 97 97 GLY N N 115.42 . . stop_ save_ save_assigned_chemical_shifts_8 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_8 stop_ _Sample_conditions_label $sample_conditions_9 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.317 . . 2 5 5 GLU N N 121.375 . . 3 6 6 ALA H H 8.341 . . 4 6 6 ALA N N 125.71 . . 5 7 7 LYS H H 8.349 . . 6 7 7 LYS N N 122.017 . . 7 9 9 SER H H 8.612 . . 8 9 9 SER N N 116.974 . . 9 10 10 THR H H 8.258 . . 10 10 10 THR N N 115.912 . . 11 11 11 GLU H H 8.374 . . 12 11 11 GLU N N 122.927 . . 13 12 12 ASP H H 8.421 . . 14 12 12 ASP N N 122.195 . . 15 13 13 LEU H H 8.408 . . 16 13 13 LEU N N 123.776 . . 17 14 14 GLY H H 8.469 . . 18 14 14 GLY N N 108.966 . . 19 15 15 ASP H H 8.221 . . 20 15 15 ASP N N 120.482 . . 21 16 16 LYS H H 8.317 . . 22 16 16 LYS N N 121.375 . . 23 17 17 LYS H H 8.421 . . 24 17 17 LYS N N 122.195 . . 25 18 18 GLU H H 8.506 . . 26 18 18 GLU N N 121.944 . . 27 19 19 GLY H H 8.471 . . 28 19 19 GLY N N 109.732 . . 29 20 20 GLU H H 8.23 . . 30 20 20 GLU N N 119.65 . . 31 21 21 TYR H H 8.326 . . 32 21 21 TYR N N 119.282 . . 33 22 22 ILE H H 9.162 . . 34 22 22 ILE N N 116.724 . . 35 23 23 LYS H H 8.632 . . 36 23 23 LYS N N 124.371 . . 37 24 24 LEU H H 8.938 . . 38 24 24 LEU N N 124.102 . . 39 25 25 LYS H H 8.64 . . 40 25 25 LYS N N 120.69 . . 41 26 26 VAL H H 9.117 . . 42 26 26 VAL N N 124.125 . . 43 27 27 ILE H H 9.08 . . 44 27 27 ILE N N 128.422 . . 45 28 28 GLY H H 8.477 . . 46 28 28 GLY N N 113.342 . . 47 29 29 GLN H H 8.776 . . 48 29 29 GLN N N 120.51 . . 49 30 30 ASP H H 8.347 . . 50 30 30 ASP N N 118.393 . . 51 31 31 SER H H 8.011 . . 52 31 31 SER N N 111.11 . . 53 32 32 SER H H 8.094 . . 54 32 32 SER N N 117.526 . . 55 33 33 GLU H H 8.617 . . 56 33 33 GLU N N 121.815 . . 57 34 34 ILE H H 8.679 . . 58 34 34 ILE N N 124.482 . . 59 35 35 HIS H H 8.734 . . 60 35 35 HIS N N 125.662 . . 61 36 36 PHE H H 9.097 . . 62 36 36 PHE N N 120.046 . . 63 37 37 LYS H H 8.801 . . 64 37 37 LYS N N 123.829 . . 65 38 38 VAL H H 9.148 . . 66 38 38 VAL N N 119.68 . . 67 39 39 LYS H H 8.634 . . 68 39 39 LYS N N 122.32 . . 69 40 40 MET H H 8.582 . . 70 40 40 MET N N 120.005 . . 71 42 42 THR H H 7.192 . . 72 42 42 THR N N 120.753 . . 73 43 43 HIS H H 8.856 . . 74 43 43 HIS N N 126.226 . . 75 44 44 LEU H H 9.361 . . 76 44 44 LEU N N 124.946 . . 77 45 45 LYS H H 8.556 . . 78 45 45 LYS N N 121.493 . . 79 46 46 LYS H H 7.523 . . 80 46 46 LYS N N 114.104 . . 81 47 47 LEU H H 6.75 . . 82 47 47 LEU N N 121.972 . . 83 48 48 LYS H H 7.564 . . 84 48 48 LYS N N 118.334 . . 85 49 49 GLU H H 8.514 . . 86 49 49 GLU N N 118.194 . . 87 50 50 SER H H 7.965 . . 88 50 50 SER N N 115.694 . . 89 51 51 TYR H H 8.843 . . 90 51 51 TYR N N 122.689 . . 91 52 52 CYS H H 8.676 . . 92 52 52 CYS N N 117.465 . . 93 53 53 GLN H H 8.326 . . 94 53 53 GLN N N 119.282 . . 95 54 54 ARG H H 8.024 . . 96 54 54 ARG N N 120.189 . . 97 55 55 GLN H H 7.808 . . 98 55 55 GLN N N 114.177 . . 99 56 56 GLY H H 7.931 . . 100 56 56 GLY N N 109.755 . . 101 57 57 VAL H H 7.663 . . 102 57 57 VAL N N 112.032 . . 103 59 59 MET H H 9.015 . . 104 59 59 MET N N 124.557 . . 105 60 60 ASN H H 8.444 . . 106 60 60 ASN N N 113.283 . . 107 61 61 SER H H 7.938 . . 108 61 61 SER N N 113.092 . . 109 62 62 LEU H H 7.274 . . 110 62 62 LEU N N 121.013 . . 111 63 63 ARG H H 9.012 . . 112 63 63 ARG N N 120.302 . . 113 64 64 PHE H H 8.944 . . 114 64 64 PHE N N 122.63 . . 115 65 65 LEU H H 9.749 . . 116 65 65 LEU N N 124.31 . . 117 66 66 PHE H H 8.973 . . 118 66 66 PHE N N 119.463 . . 119 67 67 GLU H H 9.386 . . 120 67 67 GLU N N 129.287 . . 121 68 68 GLY H H 8.874 . . 122 68 68 GLY N N 103.339 . . 123 69 69 GLN H H 7.997 . . 124 69 69 GLN N N 120.722 . . 125 70 70 ARG H H 8.834 . . 126 70 70 ARG N N 125.112 . . 127 71 71 ILE H H 9.034 . . 128 71 71 ILE N N 126.975 . . 129 73 73 ASP H H 8.776 . . 130 73 73 ASP N N 120.51 . . 131 74 74 ASN H H 7.765 . . 132 74 74 ASN N N 109.623 . . 133 75 75 HIS H H 7.163 . . 134 75 75 HIS N N 119.916 . . 135 76 76 THR H H 7.321 . . 136 76 76 THR N N 108.403 . . 137 78 78 LYS H H 8.094 . . 138 78 78 LYS N N 115.444 . . 139 79 79 GLU H H 7.997 . . 140 79 79 GLU N N 120.722 . . 141 80 80 LEU H H 7.488 . . 142 80 80 LEU N N 115.098 . . 143 81 81 GLY H H 7.658 . . 144 81 81 GLY N N 108.17 . . 145 82 82 MET H H 7.644 . . 146 82 82 MET N N 117.821 . . 147 83 83 GLU H H 9.313 . . 148 83 83 GLU N N 121.075 . . 149 84 84 GLU H H 8.326 . . 150 84 84 GLU N N 119.282 . . 151 85 85 GLU H H 9.37 . . 152 85 85 GLU N N 118.995 . . 153 86 86 ASP H H 8.157 . . 154 86 86 ASP N N 120.295 . . 155 87 87 VAL H H 8.199 . . 156 87 87 VAL N N 116.747 . . 157 88 88 ILE H H 9.167 . . 158 88 88 ILE N N 127.415 . . 159 89 89 GLU H H 8.869 . . 160 89 89 GLU N N 125.93 . . 161 90 90 VAL H H 7.78 . . 162 90 90 VAL N N 119.83 . . 163 91 91 TYR H H 8.537 . . 164 91 91 TYR N N 124.23 . . 165 92 92 GLN H H 8.739 . . 166 92 92 GLN N N 120.852 . . 167 93 93 GLU H H 8.423 . . 168 93 93 GLU N N 125.201 . . 169 94 94 GLN H H 8.8 . . 170 94 94 GLN N N 122.996 . . 171 95 95 THR H H 8.404 . . 172 95 95 THR N N 116.141 . . 173 96 96 GLY H H 8.522 . . 174 96 96 GLY N N 111.898 . . 175 97 97 GLY H H 8.036 . . 176 97 97 GLY N N 115.395 . . stop_ save_ save_assigned_chemical_shifts_9 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_9 stop_ _Sample_conditions_label $sample_conditions_10 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.128 . . 2 5 5 GLU N N 120.853 . . 3 6 6 ALA H H 8.151 . . 4 6 6 ALA N N 125.18 . . 5 7 7 LYS H H 8.157 . . 6 7 7 LYS N N 121.471 . . 7 9 9 SER H H 8.422 . . 8 9 9 SER N N 116.459 . . 9 10 10 THR H H 8.066 . . 10 10 10 THR N N 115.385 . . 11 11 11 GLU H H 8.183 . . 12 11 11 GLU N N 122.42 . . 13 12 12 ASP H H 8.23 . . 14 12 12 ASP N N 121.68 . . 15 13 13 LEU H H 8.217 . . 16 13 13 LEU N N 123.248 . . 17 14 14 GLY H H 8.281 . . 18 14 14 GLY N N 108.449 . . 19 15 15 ASP H H 8.03 . . 20 15 15 ASP N N 119.967 . . 21 16 16 LYS H H 8.128 . . 22 16 16 LYS N N 120.853 . . 23 17 17 LYS H H 8.23 . . 24 17 17 LYS N N 121.68 . . 25 18 18 GLU H H 8.317 . . 26 18 18 GLU N N 121.416 . . 27 19 19 GLY H H 8.281 . . 28 19 19 GLY N N 109.212 . . 29 20 20 GLU H H 8.042 . . 30 20 20 GLU N N 119.149 . . 31 21 21 TYR H H 8.137 . . 32 21 21 TYR N N 118.771 . . 33 22 22 ILE H H 8.973 . . 34 22 22 ILE N N 116.201 . . 35 23 23 LYS H H 8.445 . . 36 23 23 LYS N N 123.889 . . 37 24 24 LEU H H 8.752 . . 38 24 24 LEU N N 123.669 . . 39 25 25 LYS H H 8.45 . . 40 25 25 LYS N N 120.156 . . 41 26 26 VAL H H 8.931 . . 42 26 26 VAL N N 123.646 . . 43 27 27 ILE H H 8.893 . . 44 27 27 ILE N N 127.927 . . 45 28 28 GLY H H 8.256 . . 46 28 28 GLY N N 112.782 . . 47 29 29 GLN H H 8.586 . . 48 29 29 GLN N N 119.981 . . 49 30 30 ASP H H 8.156 . . 50 30 30 ASP N N 117.88 . . 51 31 31 SER H H 7.822 . . 52 31 31 SER N N 110.594 . . 53 32 32 SER H H 7.905 . . 54 32 32 SER N N 117.009 . . 55 33 33 GLU H H 8.424 . . 56 33 33 GLU N N 121.302 . . 57 34 34 ILE H H 8.487 . . 58 34 34 ILE N N 123.977 . . 59 35 35 HIS H H 8.543 . . 60 35 35 HIS N N 125.229 . . 61 36 36 PHE H H 8.915 . . 62 36 36 PHE N N 119.537 . . 63 37 37 LYS H H 8.613 . . 64 37 37 LYS N N 123.305 . . 65 38 38 VAL H H 8.957 . . 66 38 38 VAL N N 119.139 . . 67 39 39 LYS H H 8.443 . . 68 39 39 LYS N N 121.828 . . 69 40 40 MET H H 8.391 . . 70 40 40 MET N N 119.477 . . 71 42 42 THR H H 7.003 . . 72 42 42 THR N N 120.251 . . 73 43 43 HIS H H 8.663 . . 74 43 43 HIS N N 125.764 . . 75 44 44 LEU H H 9.173 . . 76 44 44 LEU N N 124.432 . . 77 45 45 LYS H H 8.365 . . 78 45 45 LYS N N 120.985 . . 79 46 46 LYS H H 7.336 . . 80 46 46 LYS N N 113.612 . . 81 47 47 LEU H H 6.562 . . 82 47 47 LEU N N 121.465 . . 83 48 48 LYS H H 7.374 . . 84 48 48 LYS N N 117.838 . . 85 49 49 GLU H H 8.327 . . 86 49 49 GLU N N 117.685 . . 87 50 50 SER H H 7.774 . . 88 50 50 SER N N 115.184 . . 89 51 51 TYR H H 8.654 . . 90 51 51 TYR N N 122.175 . . 91 52 52 CYS H H 8.486 . . 92 52 52 CYS N N 116.943 . . 93 53 53 GLN H H 8.137 . . 94 53 53 GLN N N 118.771 . . 95 54 54 ARG H H 7.837 . . 96 54 54 ARG N N 119.658 . . 97 55 55 GLN H H 7.615 . . 98 55 55 GLN N N 113.661 . . 99 56 56 GLY H H 7.741 . . 100 56 56 GLY N N 109.23 . . 101 57 57 VAL H H 7.474 . . 102 57 57 VAL N N 111.515 . . 103 59 59 MET H H 8.826 . . 104 59 59 MET N N 124.047 . . 105 60 60 ASN H H 8.256 . . 106 60 60 ASN N N 112.782 . . 107 61 61 SER H H 7.747 . . 108 61 61 SER N N 112.563 . . 109 62 62 LEU H H 7.086 . . 110 62 62 LEU N N 120.522 . . 111 63 63 ARG H H 8.823 . . 112 63 63 ARG N N 119.831 . . 113 64 64 PHE H H 8.762 . . 114 64 64 PHE N N 122.152 . . 115 65 65 LEU H H 9.562 . . 116 65 65 LEU N N 123.815 . . 117 66 66 PHE H H 8.788 . . 118 66 66 PHE N N 118.977 . . 119 67 67 GLU H H 9.198 . . 120 67 67 GLU N N 128.78 . . 121 68 68 GLY H H 8.686 . . 122 68 68 GLY N N 102.824 . . 123 69 69 GLN H H 7.808 . . 124 69 69 GLN N N 120.203 . . 125 70 70 ARG H H 8.646 . . 126 70 70 ARG N N 124.611 . . 127 71 71 ILE H H 8.843 . . 128 71 71 ILE N N 126.463 . . 129 73 73 ASP H H 8.586 . . 130 73 73 ASP N N 119.981 . . 131 74 74 ASN H H 7.576 . . 132 74 74 ASN N N 109.116 . . 133 75 75 HIS H H 6.973 . . 134 75 75 HIS N N 119.391 . . 135 76 76 THR H H 7.132 . . 136 76 76 THR N N 107.908 . . 137 78 78 LYS H H 7.906 . . 138 78 78 LYS N N 114.952 . . 139 79 79 GLU H H 7.808 . . 140 79 79 GLU N N 120.203 . . 141 80 80 LEU H H 7.295 . . 142 80 80 LEU N N 114.573 . . 143 81 81 GLY H H 7.469 . . 144 81 81 GLY N N 107.651 . . 145 82 82 MET H H 7.455 . . 146 82 82 MET N N 117.346 . . 147 83 83 GLU H H 9.125 . . 148 83 83 GLU N N 120.544 . . 149 84 84 GLU H H 8.137 . . 150 84 84 GLU N N 118.771 . . 151 85 85 GLU H H 9.177 . . 152 85 85 GLU N N 118.462 . . 153 86 86 ASP H H 7.969 . . 154 86 86 ASP N N 119.792 . . 155 87 87 VAL H H 8.009 . . 156 87 87 VAL N N 116.256 . . 157 88 88 ILE H H 8.98 . . 158 88 88 ILE N N 126.912 . . 159 89 89 GLU H H 8.685 . . 160 89 89 GLU N N 125.439 . . 161 90 90 VAL H H 7.591 . . 162 90 90 VAL N N 119.334 . . 163 91 91 TYR H H 8.345 . . 164 91 91 TYR N N 123.694 . . 165 92 92 GLN H H 8.554 . . 166 92 92 GLN N N 120.354 . . 167 93 93 GLU H H 8.232 . . 168 93 93 GLU N N 124.704 . . 169 94 94 GLN H H 8.614 . . 170 94 94 GLN N N 122.489 . . 171 95 95 THR H H 8.213 . . 172 95 95 THR N N 115.609 . . 173 96 96 GLY H H 8.332 . . 174 96 96 GLY N N 111.373 . . 175 97 97 GLY H H 7.846 . . 176 97 97 GLY N N 114.881 . . stop_ save_ save_assigned_chemical_shifts_10 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_10 stop_ _Sample_conditions_label $sample_conditions_11 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.309 . . 2 5 5 GLU N N 121.327 . . 3 6 6 ALA H H 8.329 . . 4 6 6 ALA N N 125.6 . . 5 7 7 LYS H H 8.339 . . 6 7 7 LYS N N 122.288 . . 7 9 9 SER H H 8.601 . . 8 9 9 SER N N 116.93 . . 9 10 10 THR H H 8.245 . . 10 10 10 THR N N 115.861 . . 11 11 11 GLU H H 8.364 . . 12 11 11 GLU N N 123.051 . . 13 12 12 ASP H H 8.409 . . 14 12 12 ASP N N 122.158 . . 15 13 13 LEU H H 8.398 . . 16 13 13 LEU N N 123.716 . . 17 14 14 GLY H H 8.462 . . 18 14 14 GLY N N 108.924 . . 19 15 15 ASP H H 8.212 . . 20 15 15 ASP N N 120.448 . . 21 16 16 LYS H H 8.309 . . 22 16 16 LYS N N 121.327 . . 23 17 17 LYS H H 8.409 . . 24 17 17 LYS N N 122.158 . . 25 18 18 GLU H H 8.497 . . 26 18 18 GLU N N 121.882 . . 27 19 19 GLY H H 8.46 . . 28 19 19 GLY N N 109.684 . . 29 20 20 GLU H H 8.223 . . 30 20 20 GLU N N 119.634 . . 31 21 21 TYR H H 8.319 . . 32 21 21 TYR N N 119.248 . . 33 22 22 ILE H H 9.15 . . 34 22 22 ILE N N 116.633 . . 35 23 23 LYS H H 8.633 . . 36 23 23 LYS N N 124.365 . . 37 24 24 LEU H H 8.939 . . 38 24 24 LEU N N 124.19 . . 39 25 25 LYS H H 8.631 . . 40 25 25 LYS N N 120.636 . . 41 26 26 VAL H H 9.118 . . 42 26 26 VAL N N 124.126 . . 43 27 27 ILE H H 9.082 . . 44 27 27 ILE N N 128.424 . . 45 28 28 GLY H H 8.437 . . 46 28 28 GLY N N 113.263 . . 47 29 29 GLN H H 8.768 . . 48 29 29 GLN N N 120.473 . . 49 30 30 ASP H H 8.338 . . 50 30 30 ASP N N 118.353 . . 51 31 31 SER H H 8.004 . . 52 31 31 SER N N 111.066 . . 53 32 32 SER H H 8.087 . . 54 32 32 SER N N 117.476 . . 55 33 33 GLU H H 8.605 . . 56 33 33 GLU N N 121.775 . . 57 34 34 ILE H H 8.669 . . 58 34 34 ILE N N 124.447 . . 59 35 35 HIS H H 8.727 . . 60 35 35 HIS N N 125.769 . . 61 36 36 PHE H H 9.095 . . 62 36 36 PHE N N 120.001 . . 63 37 37 LYS H H 8.797 . . 64 37 37 LYS N N 123.759 . . 65 38 38 VAL H H 9.138 . . 66 38 38 VAL N N 119.614 . . 67 39 39 LYS H H 8.624 . . 68 39 39 LYS N N 122.328 . . 69 40 40 MET H H 8.572 . . 70 40 40 MET N N 119.944 . . 71 42 42 THR H H 7.183 . . 72 42 42 THR N N 120.741 . . 73 43 43 HIS H H 8.841 . . 74 43 43 HIS N N 126.227 . . 75 44 44 LEU H H 9.349 . . 76 44 44 LEU N N 124.914 . . 77 45 45 LYS H H 8.548 . . 78 45 45 LYS N N 121.476 . . 79 46 46 LYS H H 7.519 . . 80 46 46 LYS N N 114.091 . . 81 47 47 LEU H H 6.747 . . 82 47 47 LEU N N 121.954 . . 83 48 48 LYS H H 7.555 . . 84 48 48 LYS N N 118.331 . . 85 49 49 GLU H H 8.507 . . 86 49 49 GLU N N 118.167 . . 87 50 50 SER H H 7.952 . . 88 50 50 SER N N 115.652 . . 89 51 51 TYR H H 8.836 . . 90 51 51 TYR N N 122.649 . . 91 52 52 CYS H H 8.669 . . 92 52 52 CYS N N 117.423 . . 93 53 53 GLN H H 8.319 . . 94 53 53 GLN N N 119.248 . . 95 54 54 ARG H H 8.019 . . 96 54 54 ARG N N 120.137 . . 97 55 55 GLN H H 7.797 . . 98 55 55 GLN N N 114.122 . . 99 56 56 GLY H H 7.925 . . 100 56 56 GLY N N 109.686 . . 101 57 57 VAL H H 7.657 . . 102 57 57 VAL N N 111.994 . . 103 59 59 MET H H 9.009 . . 104 59 59 MET N N 124.533 . . 105 60 60 ASN H H 8.437 . . 106 60 60 ASN N N 113.263 . . 107 61 61 SER H H 7.928 . . 108 61 61 SER N N 113.035 . . 109 62 62 LEU H H 7.269 . . 110 62 62 LEU N N 121.007 . . 111 63 63 ARG H H 9.004 . . 112 63 63 ARG N N 120.32 . . 113 64 64 PHE H H 8.939 . . 114 64 64 PHE N N 122.645 . . 115 65 65 LEU H H 9.746 . . 116 65 65 LEU N N 124.337 . . 117 66 66 PHE H H 8.975 . . 118 66 66 PHE N N 119.478 . . 119 67 67 GLU H H 9.384 . . 120 67 67 GLU N N 129.271 . . 121 68 68 GLY H H 8.862 . . 122 68 68 GLY N N 103.289 . . 123 69 69 GLN H H 7.992 . . 124 69 69 GLN N N 120.677 . . 125 70 70 ARG H H 8.829 . . 126 70 70 ARG N N 125.129 . . 127 71 71 ILE H H 9.025 . . 128 71 71 ILE N N 126.944 . . 129 73 73 ASP H H 8.768 . . 130 73 73 ASP N N 120.473 . . 131 74 74 ASN H H 7.758 . . 132 74 74 ASN N N 109.592 . . 133 75 75 HIS H H 7.154 . . 134 75 75 HIS N N 119.884 . . 135 76 76 THR H H 7.315 . . 136 76 76 THR N N 108.395 . . 137 78 78 LYS H H 8.023 . . 138 78 78 LYS N N 115.352 . . 139 79 79 GLU H H 7.96 . . 140 79 79 GLU N N 120.838 . . 141 80 80 LEU H H 7.475 . . 142 80 80 LEU N N 115.037 . . 143 81 81 GLY H H 7.651 . . 144 81 81 GLY N N 108.16 . . 145 82 82 MET H H 7.645 . . 146 82 82 MET N N 117.885 . . 147 83 83 GLU H H 9.304 . . 148 83 83 GLU N N 121.055 . . 149 84 84 GLU H H 8.319 . . 150 84 84 GLU N N 119.248 . . 151 85 85 GLU H H 9.363 . . 152 85 85 GLU N N 118.943 . . 153 86 86 ASP H H 8.149 . . 154 86 86 ASP N N 120.25 . . 155 87 87 VAL H H 8.192 . . 156 87 87 VAL N N 116.723 . . 157 88 88 ILE H H 9.165 . . 158 88 88 ILE N N 127.411 . . 159 89 89 GLU H H 8.87 . . 160 89 89 GLU N N 125.922 . . 161 90 90 VAL H H 7.776 . . 162 90 90 VAL N N 119.839 . . 163 91 91 TYR H H 8.527 . . 164 91 91 TYR N N 124.152 . . 165 92 92 GLN H H 8.737 . . 166 92 92 GLN N N 120.835 . . 167 93 93 GLU H H 8.413 . . 168 93 93 GLU N N 125.177 . . 169 94 94 GLN H H 8.795 . . 170 94 94 GLN N N 122.97 . . 171 95 95 THR H H 8.391 . . 172 95 95 THR N N 116.078 . . 173 96 96 GLY H H 8.512 . . 174 96 96 GLY N N 111.848 . . 175 97 97 GLY H H 8.023 . . 176 97 97 GLY N N 115.352 . . stop_ save_ save_assigned_chemical_shifts_11 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_13 stop_ _Sample_conditions_label $sample_conditions_12 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.308 . . 2 5 5 GLU N N 121.369 . . 3 6 6 ALA H H 8.332 . . 4 6 6 ALA N N 125.700 . . 5 7 7 LYS H H 8.355 . . 6 7 7 LYS N N 122.301 . . 7 9 9 SER H H 8.601 . . 8 9 9 SER N N 116.972 . . 9 10 10 THR H H 8.248 . . 10 10 10 THR N N 115.907 . . 11 11 11 GLU H H 8.364 . . 12 11 11 GLU N N 123.097 . . 13 12 12 ASP H H 8.411 . . 14 12 12 ASP N N 122.196 . . 15 13 13 LEU H H 8.399 . . 16 13 13 LEU N N 123.774 . . 17 14 14 GLY H H 8.459 . . 18 14 14 GLY N N 108.959 . . 19 15 15 ASP H H 8.211 . . 20 15 15 ASP N N 120.477 . . 21 16 16 LYS H H 8.308 . . 22 16 16 LYS N N 121.369 . . 23 17 17 LYS H H 8.411 . . 24 17 17 LYS N N 122.196 . . 25 18 18 GLU H H 8.497 . . 26 18 18 GLU N N 121.939 . . 27 19 19 GLY H H 8.461 . . 28 19 19 GLY N N 109.727 . . 29 20 20 GLU H H 8.220 . . 30 20 20 GLU N N 119.638 . . 31 21 21 TYR H H 8.318 . . 32 21 21 TYR N N 119.258 . . 33 22 22 ILE H H 9.156 . . 34 22 22 ILE N N 116.736 . . 35 23 23 LYS H H 8.621 . . 36 23 23 LYS N N 124.359 . . 37 24 24 LEU H H 8.928 . . 38 24 24 LEU N N 124.088 . . 39 25 25 LYS H H 8.634 . . 40 25 25 LYS N N 120.699 . . 41 26 26 VAL H H 9.104 . . 42 26 26 VAL N N 124.126 . . 43 27 27 ILE H H 9.072 . . 44 27 27 ILE N N 128.404 . . 45 28 28 GLY H H 8.467 . . 46 28 28 GLY N N 113.327 . . 47 29 29 GLN H H 8.769 . . 48 29 29 GLN N N 120.512 . . 49 30 30 ASP H H 8.337 . . 50 30 30 ASP N N 118.383 . . 51 31 31 SER H H 8.001 . . 52 31 31 SER N N 111.118 . . 53 32 32 SER H H 8.085 . . 54 32 32 SER N N 117.526 . . 55 33 33 GLU H H 8.609 . . 56 33 33 GLU N N 121.813 . . 57 34 34 ILE H H 8.668 . . 58 34 34 ILE N N 124.478 . . 59 35 35 HIS H H 8.725 . . 60 35 35 HIS N N 125.653 . . 61 36 36 PHE H H 9.091 . . 62 36 36 PHE N N 120.045 . . 63 37 37 LYS H H 8.791 . . 64 37 37 LYS N N 123.833 . . 65 38 38 VAL H H 9.139 . . 66 38 38 VAL N N 119.693 . . 67 39 39 LYS H H 8.628 . . 68 39 39 LYS N N 122.323 . . 69 40 40 MET H H 8.574 . . 70 40 40 MET N N 120.000 . . 71 42 42 THR H H 7.180 . . 72 42 42 THR N N 120.741 . . 73 43 43 HIS H H 8.846 . . 74 43 43 HIS N N 126.241 . . 75 44 44 LEU H H 9.353 . . 76 44 44 LEU N N 124.974 . . 77 45 45 LYS H H 8.547 . . 78 45 45 LYS N N 121.486 . . 79 46 46 LYS H H 7.511 . . 80 46 46 LYS N N 114.079 . . 81 47 47 LEU H H 6.740 . . 82 47 47 LEU N N 121.970 . . 83 48 48 LYS H H 7.553 . . 84 48 48 LYS N N 118.327 . . 85 49 49 GLU H H 8.503 . . 86 49 49 GLU N N 118.180 . . 87 50 50 SER H H 7.956 . . 88 50 50 SER N N 115.693 . . 89 51 51 TYR H H 8.834 . . 90 51 51 TYR N N 122.685 . . 91 52 52 CYS H H 8.669 . . 92 52 52 CYS N N 117.448 . . 93 53 53 GLN H H 8.323 . . 94 53 53 GLN N N 119.221 . . 95 54 54 ARG H H 8.014 . . 96 54 54 ARG N N 120.194 . . 97 55 55 GLN H H 7.796 . . 98 55 55 GLN N N 114.169 . . 99 56 56 GLY H H 7.924 . . 100 56 56 GLY N N 109.752 . . 101 57 57 VAL H H 7.655 . . 102 57 57 VAL N N 112.022 . . 103 59 59 MET H H 9.007 . . 104 59 59 MET N N 124.560 . . 105 60 60 ASN H H 8.433 . . 106 60 60 ASN N N 113.267 . . 107 61 61 SER H H 7.927 . . 108 61 61 SER N N 113.087 . . 109 62 62 LEU H H 7.262 . . 110 62 62 LEU N N 121.014 . . 111 63 63 ARG H H 9.002 . . 112 63 63 ARG N N 120.288 . . 113 64 64 PHE H H 8.933 . . 114 64 64 PHE N N 122.643 . . 115 65 65 LEU H H 9.741 . . 116 65 65 LEU N N 124.280 . . 117 66 66 PHE H H 8.959 . . 118 66 66 PHE N N 119.446 . . 119 67 67 GLU H H 9.369 . . 120 67 67 GLU N N 129.271 . . 121 68 68 GLY H H 8.869 . . 122 68 68 GLY N N 103.346 . . 123 69 69 GLN H H 7.986 . . 124 69 69 GLN N N 120.721 . . 125 70 70 ARG H H 8.822 . . 126 70 70 ARG N N 125.077 . . 127 71 71 ILE H H 9.028 . . 128 71 71 ILE N N 126.981 . . 129 73 73 ASP H H 8.769 . . 130 73 73 ASP N N 120.512 . . 131 74 74 ASN H H 7.755 . . 132 74 74 ASN N N 109.614 . . 133 75 75 HIS H H 7.153 . . 134 75 75 HIS N N 119.910 . . 135 76 76 THR H H 7.310 . . 136 76 76 THR N N 108.424 . . 137 78 78 LYS H H 8.082 . . 138 78 78 LYS N N 115.417 . . 139 79 79 GLU H H 7.962 . . 140 79 79 GLU N N 120.868 . . 141 80 80 LEU H H 7.478 . . 142 80 80 LEU N N 115.118 . . 143 81 81 GLY H H 7.644 . . 144 81 81 GLY N N 108.128 . . 145 82 82 MET H H 7.625 . . 146 82 82 MET N N 117.769 . . 147 83 83 GLU H H 9.304 . . 148 83 83 GLU N N 121.065 . . 149 84 84 GLU H H 8.323 . . 150 84 84 GLU N N 119.221 . . 151 85 85 GLU H H 9.360 . . 152 85 85 GLU N N 118.950 . . 153 86 86 ASP H H 8.148 . . 154 86 86 ASP N N 120.296 . . 155 87 87 VAL H H 8.197 . . 156 87 87 VAL N N 116.757 . . 157 88 88 ILE H H 9.155 . . 158 88 88 ILE N N 127.385 . . 159 89 89 GLU H H 8.858 . . 160 89 89 GLU N N 125.906 . . 161 90 90 VAL H H 7.772 . . 162 90 90 VAL N N 119.835 . . 163 91 91 TYR H H 8.530 . . 164 91 91 TYR N N 124.217 . . 165 92 92 GLN H H 8.731 . . 166 92 92 GLN N N 120.841 . . 167 93 93 GLU H H 8.412 . . 168 93 93 GLU N N 125.189 . . 169 94 94 GLN H H 8.791 . . 170 94 94 GLN N N 122.987 . . 171 95 95 THR H H 8.394 . . 172 95 95 THR N N 116.140 . . 173 96 96 GLY H H 8.512 . . 174 96 96 GLY N N 111.896 . . 175 97 97 GLY H H 8.026 . . 176 97 97 GLY N N 115.395 . . stop_ save_ save_assigned_chemical_shifts_12 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_11 stop_ _Sample_conditions_label $sample_conditions_13 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.316 . . 2 5 5 GLU N N 121.369 . . 3 6 6 ALA H H 8.342 . . 4 6 6 ALA N N 125.700 . . 5 7 7 LYS H H 8.361 . . 6 7 7 LYS N N 122.298 . . 7 9 9 SER H H 8.611 . . 8 9 9 SER N N 116.962 . . 9 10 10 THR H H 8.257 . . 10 10 10 THR N N 115.902 . . 11 11 11 GLU H H 8.373 . . 12 11 11 GLU N N 123.090 . . 13 12 12 ASP H H 8.419 . . 14 12 12 ASP N N 122.188 . . 15 13 13 LEU H H 8.407 . . 16 13 13 LEU N N 123.764 . . 17 14 14 GLY H H 8.468 . . 18 14 14 GLY N N 108.959 . . 19 15 15 ASP H H 8.220 . . 20 15 15 ASP N N 120.470 . . 21 16 16 LYS H H 8.316 . . 22 16 16 LYS N N 121.369 . . 23 17 17 LYS H H 8.419 . . 24 17 17 LYS N N 122.188 . . 25 18 18 GLU H H 8.505 . . 26 18 18 GLU N N 121.930 . . 27 19 19 GLY H H 8.470 . . 28 19 19 GLY N N 109.724 . . 29 20 20 GLU H H 8.229 . . 30 20 20 GLU N N 119.626 . . 31 21 21 TYR H H 8.325 . . 32 21 21 TYR N N 119.269 . . 33 22 22 ILE H H 9.167 . . 34 22 22 ILE N N 116.730 . . 35 23 23 LYS H H 8.630 . . 36 23 23 LYS N N 124.353 . . 37 24 24 LEU H H 8.935 . . 38 24 24 LEU N N 124.089 . . 39 25 25 LYS H H 8.646 . . 40 25 25 LYS N N 120.702 . . 41 26 26 VAL H H 9.113 . . 42 26 26 VAL N N 124.123 . . 43 27 27 ILE H H 9.081 . . 44 27 27 ILE N N 128.405 . . 45 28 28 GLY H H 8.477 . . 46 28 28 GLY N N 113.327 . . 47 29 29 GLN H H 8.774 . . 48 29 29 GLN N N 120.483 . . 49 30 30 ASP H H 8.346 . . 50 30 30 ASP N N 118.389 . . 51 31 31 SER H H 8.010 . . 52 31 31 SER N N 111.109 . . 53 32 32 SER H H 8.093 . . 54 32 32 SER N N 117.521 . . 55 33 33 GLU H H 8.618 . . 56 33 33 GLU N N 121.812 . . 57 34 34 ILE H H 8.676 . . 58 34 34 ILE N N 124.481 . . 59 35 35 HIS H H 8.734 . . 60 35 35 HIS N N 125.654 . . 61 36 36 PHE H H 9.099 . . 62 36 36 PHE N N 120.053 . . 63 37 37 LYS H H 8.800 . . 64 37 37 LYS N N 123.826 . . 65 38 38 VAL H H 9.147 . . 66 38 38 VAL N N 119.690 . . 67 39 39 LYS H H 8.630 . . 68 39 39 LYS N N 122.299 . . 69 40 40 MET H H 8.581 . . 70 40 40 MET N N 119.970 . . 71 42 42 THR H H 7.192 . . 72 42 42 THR N N 120.745 . . 73 43 43 HIS H H 8.851 . . 74 43 43 HIS N N 126.210 . . 75 44 44 LEU H H 9.363 . . 76 44 44 LEU N N 124.950 . . 77 45 45 LYS H H 8.554 . . 78 45 45 LYS N N 121.477 . . 79 46 46 LYS H H 7.523 . . 80 46 46 LYS N N 114.103 . . 81 47 47 LEU H H 6.747 . . 82 47 47 LEU N N 121.950 . . 83 48 48 LYS H H 7.563 . . 84 48 48 LYS N N 118.316 . . 85 49 49 GLU H H 8.514 . . 86 49 49 GLU N N 118.164 . . 87 50 50 SER H H 7.964 . . 88 50 50 SER N N 115.697 . . 89 51 51 TYR H H 8.842 . . 90 51 51 TYR N N 122.691 . . 91 52 52 CYS H H 8.675 . . 92 52 52 CYS N N 117.439 . . 93 53 53 GLN H H 8.325 . . 94 53 53 GLN N N 119.269 . . 95 54 54 ARG H H 8.023 . . 96 54 54 ARG N N 120.184 . . 97 55 55 GLN H H 7.810 . . 98 55 55 GLN N N 114.168 . . 99 56 56 GLY H H 7.935 . . 100 56 56 GLY N N 109.751 . . 101 57 57 VAL H H 7.663 . . 102 57 57 VAL N N 112.017 . . 103 59 59 MET H H 9.012 . . 104 59 59 MET N N 124.544 . . 105 60 60 ASN H H 8.444 . . 106 60 60 ASN N N 113.275 . . 107 61 61 SER H H 7.938 . . 108 61 61 SER N N 113.075 . . 109 62 62 LEU H H 7.273 . . 110 62 62 LEU N N 121.005 . . 111 63 63 ARG H H 9.013 . . 112 63 63 ARG N N 120.286 . . 113 64 64 PHE H H 8.942 . . 114 64 64 PHE N N 122.622 . . 115 65 65 LEU H H 9.748 . . 116 65 65 LEU N N 124.305 . . 117 66 66 PHE H H 8.968 . . 118 66 66 PHE N N 119.442 . . 119 67 67 GLU H H 9.381 . . 120 67 67 GLU N N 129.270 . . 121 68 68 GLY H H 8.879 . . 122 68 68 GLY N N 103.341 . . 123 69 69 GLN H H 7.996 . . 124 69 69 GLN N N 120.727 . . 125 70 70 ARG H H 8.831 . . 126 70 70 ARG N N 125.072 . . 127 71 71 ILE H H 9.034 . . 128 71 71 ILE N N 126.969 . . 129 73 73 ASP H H 8.774 . . 130 73 73 ASP N N 120.483 . . 131 74 74 ASN H H 7.765 . . 132 74 74 ASN N N 109.609 . . 133 75 75 HIS H H 7.161 . . 134 75 75 HIS N N 119.903 . . 135 76 76 THR H H 7.321 . . 136 76 76 THR N N 108.406 . . 137 78 78 LYS H H 8.084 . . 138 78 78 LYS N N 115.417 . . 139 79 79 GLU H H 7.971 . . 140 79 79 GLU N N 120.848 . . 141 80 80 LEU H H 7.488 . . 142 80 80 LEU N N 115.106 . . 143 81 81 GLY H H 7.654 . . 144 81 81 GLY N N 108.131 . . 145 82 82 MET H H 7.633 . . 146 82 82 MET N N 117.770 . . 147 83 83 GLU H H 9.316 . . 148 83 83 GLU N N 121.072 . . 149 84 84 GLU H H 8.325 . . 150 84 84 GLU N N 119.269 . . 151 85 85 GLU H H 9.369 . . 152 85 85 GLU N N 118.946 . . 153 86 86 ASP H H 8.155 . . 154 86 86 ASP N N 120.285 . . 155 87 87 VAL H H 8.204 . . 156 87 87 VAL N N 116.733 . . 157 88 88 ILE H H 9.166 . . 158 88 88 ILE N N 127.384 . . 159 89 89 GLU H H 8.868 . . 160 89 89 GLU N N 125.887 . . 161 90 90 VAL H H 7.782 . . 162 90 90 VAL N N 119.838 . . 163 91 91 TYR H H 8.540 . . 164 91 91 TYR N N 124.201 . . 165 92 92 GLN H H 8.737 . . 166 92 92 GLN N N 120.834 . . 167 93 93 GLU H H 8.424 . . 168 93 93 GLU N N 125.198 . . 169 94 94 GLN H H 8.799 . . 170 94 94 GLN N N 122.981 . . 171 95 95 THR H H 8.402 . . 172 95 95 THR N N 116.135 . . 173 96 96 GLY H H 8.520 . . 174 96 96 GLY N N 111.893 . . 175 97 97 GLY H H 8.035 . . 176 97 97 GLY N N 115.394 . . stop_ save_ save_assigned_chemical_shifts_13 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_12 stop_ _Sample_conditions_label $sample_conditions_13 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name SUMO1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 5 5 GLU H H 8.316 . . 2 5 5 GLU N N 121.366 . . 3 6 6 ALA H H 8.34 . . 4 6 6 ALA N N 125.7 . . 5 7 7 LYS H H 8.355 . . 6 7 7 LYS N N 122.298 . . 7 9 9 SER H H 8.61 . . 8 9 9 SER N N 116.969 . . 9 10 10 THR H H 8.256 . . 10 10 10 THR N N 115.905 . . 11 11 11 GLU H H 8.372 . . 12 11 11 GLU N N 123.097 . . 13 12 12 ASP H H 8.419 . . 14 12 12 ASP N N 122.191 . . 15 13 13 LEU H H 8.407 . . 16 13 13 LEU N N 123.772 . . 17 14 14 GLY H H 8.467 . . 18 14 14 GLY N N 108.966 . . 19 15 15 ASP H H 8.22 . . 20 15 15 ASP N N 120.478 . . 21 16 16 LYS H H 8.316 . . 22 16 16 LYS N N 121.366 . . 23 17 17 LYS H H 8.419 . . 24 17 17 LYS N N 122.191 . . 25 18 18 GLU H H 8.505 . . 26 18 18 GLU N N 121.938 . . 27 19 19 GLY H H 8.469 . . 28 19 19 GLY N N 109.726 . . 29 20 20 GLU H H 8.227 . . 30 20 20 GLU N N 119.629 . . 31 21 21 TYR H H 8.323 . . 32 21 21 TYR N N 119.293 . . 33 22 22 ILE H H 9.164 . . 34 22 22 ILE N N 116.72 . . 35 23 23 LYS H H 8.629 . . 36 23 23 LYS N N 124.368 . . 37 24 24 LEU H H 8.937 . . 38 24 24 LEU N N 124.076 . . 39 25 25 LYS H H 8.643 . . 40 25 25 LYS N N 120.708 . . 41 26 26 VAL H H 9.114 . . 42 26 26 VAL N N 124.112 . . 43 27 27 ILE H H 9.082 . . 44 27 27 ILE N N 128.403 . . 45 28 28 GLY H H 8.467 . . 46 28 28 GLY N N 113.327 . . 47 29 29 GLN H H 8.775 . . 48 29 29 GLN N N 120.508 . . 49 30 30 ASP H H 8.345 . . 50 30 30 ASP N N 118.381 . . 51 31 31 SER H H 8.009 . . 52 31 31 SER N N 111.11 . . 53 32 32 SER H H 8.092 . . 54 32 32 SER N N 117.519 . . 55 33 33 GLU H H 8.615 . . 56 33 33 GLU N N 121.816 . . 57 34 34 ILE H H 8.676 . . 58 34 34 ILE N N 124.481 . . 59 35 35 HIS H H 8.732 . . 60 35 35 HIS N N 125.648 . . 61 36 36 PHE H H 9.101 . . 62 36 36 PHE N N 120.037 . . 63 37 37 LYS H H 8.8 . . 64 37 37 LYS N N 123.819 . . 65 38 38 VAL H H 9.147 . . 66 38 38 VAL N N 119.675 . . 67 39 39 LYS H H 8.632 . . 68 39 39 LYS N N 122.313 . . 69 40 40 MET H H 8.585 . . 70 40 40 MET N N 119.981 . . 71 42 42 THR H H 7.19 . . 72 42 42 THR N N 120.743 . . 73 43 43 HIS H H 8.854 . . 74 43 43 HIS N N 126.245 . . 75 44 44 LEU H H 9.363 . . 76 44 44 LEU N N 124.944 . . 77 45 45 LYS H H 8.554 . . 78 45 45 LYS N N 121.485 . . 79 46 46 LYS H H 7.521 . . 80 46 46 LYS N N 114.096 . . 81 47 47 LEU H H 6.746 . . 82 47 47 LEU N N 121.967 . . 83 48 48 LYS H H 7.563 . . 84 48 48 LYS N N 118.315 . . 85 49 49 GLU H H 8.515 . . 86 49 49 GLU N N 118.176 . . 87 50 50 SER H H 7.964 . . 88 50 50 SER N N 115.703 . . 89 51 51 TYR H H 8.843 . . 90 51 51 TYR N N 122.703 . . 91 52 52 CYS H H 8.675 . . 92 52 52 CYS N N 117.448 . . 93 53 53 GLN H H 8.323 . . 94 53 53 GLN N N 119.293 . . 95 54 54 ARG H H 8.023 . . 96 54 54 ARG N N 120.181 . . 97 55 55 GLN H H 7.809 . . 98 55 55 GLN N N 114.163 . . 99 56 56 GLY H H 7.933 . . 100 56 56 GLY N N 109.753 . . 101 57 57 VAL H H 7.662 . . 102 57 57 VAL N N 112.044 . . 103 59 59 MET H H 9.014 . . 104 59 59 MET N N 124.553 . . 105 60 60 ASN H H 8.44 . . 106 60 60 ASN N N 113.273 . . 107 61 61 SER H H 7.936 . . 108 61 61 SER N N 113.081 . . 109 62 62 LEU H H 7.272 . . 110 62 62 LEU N N 121.017 . . 111 63 63 ARG H H 9.01 . . 112 63 63 ARG N N 120.29 . . 113 64 64 PHE H H 8.944 . . 114 64 64 PHE N N 122.623 . . 115 65 65 LEU H H 9.749 . . 116 65 65 LEU N N 124.281 . . 117 66 66 PHE H H 8.967 . . 118 66 66 PHE N N 119.453 . . 119 67 67 GLU H H 9.378 . . 120 67 67 GLU N N 129.281 . . 121 68 68 GLY H H 8.859 . . 122 68 68 GLY N N 103.326 . . 123 69 69 GLN H H 7.997 . . 124 69 69 GLN N N 120.724 . . 125 70 70 ARG H H 8.831 . . 126 70 70 ARG N N 125.074 . . 127 71 71 ILE H H 9.038 . . 128 71 71 ILE N N 126.964 . . 129 73 73 ASP H H 8.775 . . 130 73 73 ASP N N 120.508 . . 131 74 74 ASN H H 7.765 . . 132 74 74 ASN N N 109.615 . . 133 75 75 HIS H H 7.16 . . 134 75 75 HIS N N 119.912 . . 135 76 76 THR H H 7.32 . . 136 76 76 THR N N 108.397 . . 137 78 78 LYS H H 8.082 . . 138 78 78 LYS N N 115.417 . . 139 79 79 GLU H H 7.969 . . 140 79 79 GLU N N 120.856 . . 141 80 80 LEU H H 7.488 . . 142 80 80 LEU N N 115.12 . . 143 81 81 GLY H H 7.655 . . 144 81 81 GLY N N 108.133 . . 145 82 82 MET H H 7.63 . . 146 82 82 MET N N 117.771 . . 147 83 83 GLU H H 9.311 . . 148 83 83 GLU N N 121.073 . . 149 84 84 GLU H H 8.323 . . 150 84 84 GLU N N 119.293 . . 151 85 85 GLU H H 9.372 . . 152 85 85 GLU N N 118.946 . . 153 86 86 ASP H H 8.155 . . 154 86 86 ASP N N 120.286 . . 155 87 87 VAL H H 8.204 . . 156 87 87 VAL N N 116.746 . . 157 88 88 ILE H H 9.163 . . 158 88 88 ILE N N 127.407 . . 159 89 89 GLU H H 8.869 . . 160 89 89 GLU N N 125.916 . . 161 90 90 VAL H H 7.779 . . 162 90 90 VAL N N 119.831 . . 163 91 91 TYR H H 8.536 . . 164 91 91 TYR N N 124.224 . . 165 92 92 GLN H H 8.739 . . 166 92 92 GLN N N 120.841 . . 167 93 93 GLU H H 8.423 . . 168 93 93 GLU N N 125.184 . . 169 94 94 GLN H H 8.798 . . 170 94 94 GLN N N 122.986 . . 171 95 95 THR H H 8.401 . . 172 95 95 THR N N 116.139 . . 173 96 96 GLY H H 8.519 . . 174 96 96 GLY N N 111.897 . . 175 97 97 GLY H H 8.034 . . 176 97 97 GLY N N 115.397 . . stop_ save_