data_50120 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Intramolecular synergy enhances the microtubule end-binding affinity of EB1 ; _BMRB_accession_number 50120 _BMRB_flat_file_name bmr50120.str _Entry_type original _Submission_date 2019-12-10 _Accession_date 2019-12-10 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Xue Yi . . 2 Pan Ying . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 heteronucl_NOE 1 T1_relaxation 1 T2_relaxation 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 50 "13C chemical shifts" 116 "15N chemical shifts" 51 "T1 relaxation values" 45 "T2 relaxation values" 47 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-02-19 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 50119 'EB1 linker-wt' stop_ _Original_release_date 2020-01-29 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Intramolecular synergy enhances the microtubule end-binding affinity of EB1 ; _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Liang . . . 2 Song . . . 3 Zhang . . . 4 Pan . . . 5 He . . . 6 Wang . . . 7 Chen . . . 8 Guo . . . 9 Deng . . . 10 Xue Yi . . 11 Fang Ying . . stop_ _Journal_abbreviation 'Not known' _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly_1 _Saveframe_category molecular_system _Mol_system_name EB1linker-4D _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label EB1linker-4D $entity_1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 61 _Mol_residue_sequence ; QGQETAVAPSLVAPALNKPK KPLDDDDAAPQRPISTQRTA AAPKAGPGVVRKNPGVGNGD D ; loop_ _Residue_seq_code _Residue_label 1 GLN 2 GLY 3 GLN 4 GLU 5 THR 6 ALA 7 VAL 8 ALA 9 PRO 10 SER 11 LEU 12 VAL 13 ALA 14 PRO 15 ALA 16 LEU 17 ASN 18 LYS 19 PRO 20 LYS 21 LYS 22 PRO 23 LEU 24 ASP 25 ASP 26 ASP 27 ASP 28 ALA 29 ALA 30 PRO 31 GLN 32 ARG 33 PRO 34 ILE 35 SER 36 THR 37 GLN 38 ARG 39 THR 40 ALA 41 ALA 42 ALA 43 PRO 44 LYS 45 ALA 46 GLY 47 PRO 48 GLY 49 VAL 50 VAL 51 ARG 52 LYS 53 ASN 54 PRO 55 GLY 56 VAL 57 GLY 58 ASN 59 GLY 60 ASP 61 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source_1 _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 'E. coli' 562 Bacteria . Escherichia coli stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . Escherichia coli . plasmid pet28a-SUMO stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM '[U-99% 15N]' 'sodium chloride' 50 mM 'natural abundance' 'sodium phosphate' 20 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' 'sodium azide' 0.01 % 'natural abundance' DSS 20 uM 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1 mM '[U-99% 13C; U-99% 15N]' 'sodium chloride' 50 mM 'natural abundance' 'sodium phosphate' 20 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' 'sodium azide' 0.01 % 'natural abundance' DSS 20 uM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name TOPSPIN _Version 3.01 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_software_2 _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task 'data analysis' processing stop_ _Details . save_ save_software_3 _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'AVANCE III HD' _Field_strength 800 _Details 'with cryoprobe' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_2 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_2 save_ save_15N-(1H)_NOE_4 _Saveframe_category NMR_applied_experiment _Experiment_name '15N-(1H) NOE' _Sample_label $sample_1 save_ save_15N_R1_5 _Saveframe_category NMR_applied_experiment _Experiment_name '15N R1' _Sample_label $sample_1 save_ save_15N_R2_6 _Saveframe_category NMR_applied_experiment _Experiment_name '15N R2' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.17 . M pH 6.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 internal indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name EB1linker-4D _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 GLN CA C 59.966 0.000 1 2 1 1 GLN CB C 27.789 0.000 1 3 2 2 GLY H H 8.633 0.000 1 4 2 2 GLY CA C 45.246 0.000 1 5 2 2 GLY N N 109.642 0.000 1 6 3 3 GLN H H 8.560 0.000 1 7 3 3 GLN CA C 55.918 0.000 1 8 3 3 GLN CB C 29.346 0.000 1 9 3 3 GLN N N 120.621 0.000 1 10 4 4 GLU H H 8.730 0.000 1 11 4 4 GLU CA C 56.817 0.000 1 12 4 4 GLU CB C 30.125 0.000 1 13 4 4 GLU N N 122.679 0.000 1 14 5 5 THR H H 8.249 0.000 1 15 5 5 THR CA C 61.873 0.000 1 16 5 5 THR CB C 69.939 0.000 1 17 5 5 THR N N 115.659 0.000 1 18 6 6 ALA H H 8.376 0.000 1 19 6 6 ALA CA C 52.377 0.000 1 20 6 6 ALA CB C 19.344 0.000 1 21 6 6 ALA N N 127.248 0.000 1 22 7 7 VAL H H 8.153 0.000 1 23 7 7 VAL CA C 61.873 0.000 1 24 7 7 VAL CB C 32.888 0.000 1 25 7 7 VAL N N 119.961 0.000 1 26 8 8 ALA H H 8.435 0.000 1 27 8 8 ALA CA C 50.488 0.000 1 28 8 8 ALA CB C 18.056 0.000 1 29 8 8 ALA N N 129.715 0.000 1 30 9 9 PRO CA C 63.313 0.000 1 31 9 9 PRO CB C 32.071 0.000 1 32 10 10 SER H H 8.366 0.000 1 33 10 10 SER CA C 58.312 0.000 1 34 10 10 SER CB C 63.926 0.000 1 35 10 10 SER N N 115.539 0.000 1 36 11 11 LEU H H 8.347 0.000 1 37 11 11 LEU CA C 55.218 0.000 1 38 11 11 LEU CB C 42.462 0.000 1 39 11 11 LEU N N 124.513 0.000 1 40 12 12 VAL H H 8.046 0.000 1 41 12 12 VAL CA C 61.912 0.000 1 42 12 12 VAL CB C 32.693 0.000 1 43 12 12 VAL N N 120.983 0.000 1 44 13 13 ALA H H 8.383 0.000 1 45 13 13 ALA CA C 50.490 0.000 1 46 13 13 ALA CB C 18.088 0.000 1 47 13 13 ALA N N 129.627 0.000 1 48 14 14 PRO CA C 63.157 0.000 1 49 14 14 PRO CB C 31.993 0.000 1 50 15 15 ALA H H 8.382 0.000 1 51 15 15 ALA CA C 52.279 0.000 1 52 15 15 ALA CB C 19.013 0.000 1 53 15 15 ALA N N 123.969 0.000 1 54 16 16 LEU H H 8.241 0.000 1 55 16 16 LEU CA C 55.023 0.000 1 56 16 16 LEU CB C 42.540 0.000 1 57 16 16 LEU N N 121.356 0.000 1 58 17 17 ASN H H 8.411 0.000 1 59 17 17 ASN CA C 52.999 0.000 1 60 17 17 ASN CB C 38.882 0.000 1 61 17 17 ASN N N 119.478 0.000 1 62 18 18 LYS H H 8.213 0.000 1 63 18 18 LYS CA C 54.257 0.000 1 64 18 18 LYS CB C 32.540 0.000 1 65 18 18 LYS N N 122.880 0.000 1 66 19 19 PRO CA C 63.041 0.000 1 67 19 19 PRO CB C 32.032 0.000 1 68 20 20 LYS H H 8.501 0.000 1 69 20 20 LYS CA C 55.918 0.000 1 70 20 20 LYS CB C 30.864 0.000 1 71 20 20 LYS N N 122.384 0.000 1 72 21 21 LYS H H 8.489 0.000 1 73 21 21 LYS CA C 56.240 0.000 1 74 21 21 LYS CB C 32.895 0.000 1 75 21 21 LYS N N 122.862 0.000 1 76 22 22 PRO CA C 63.235 0.000 1 77 22 22 PRO CB C 31.993 0.000 1 78 23 23 LEU H H 8.368 0.000 1 79 23 23 LEU CA C 54.517 0.000 1 80 23 23 LEU CB C 41.372 0.000 1 81 23 23 LEU N N 121.668 0.000 1 82 24 24 ASP H H 8.358 0.000 1 83 24 24 ASP CA C 45.314 0.000 1 84 24 24 ASP CB C 31.954 0.000 1 85 24 24 ASP N N 120.979 0.000 1 86 25 25 ASP CA C 54.284 0.000 1 87 25 25 ASP CB C 41.178 0.000 1 88 25 25 ASP N N 121.044 0.000 1 89 26 26 ASP H H 8.260 0.000 1 90 26 26 ASP CA C 55.179 0.000 1 91 26 26 ASP CB C 42.345 0.000 1 92 26 26 ASP N N 120.462 0.000 1 93 27 27 ASP H H 8.313 0.000 1 94 27 27 ASP CA C 54.595 0.000 1 95 27 27 ASP CB C 41.178 0.000 1 96 27 27 ASP N N 120.709 0.000 1 97 28 28 ALA H H 8.106 0.000 1 98 28 28 ALA CA C 52.221 0.000 1 99 28 28 ALA CB C 19.149 0.000 1 100 28 28 ALA N N 123.542 0.000 1 101 29 29 ALA H H 8.131 0.000 1 102 29 29 ALA CA C 50.803 0.000 1 103 29 29 ALA CB C 18.069 0.000 1 104 29 29 ALA N N 124.382 0.000 1 105 30 30 PRO CA C 63.391 0.000 1 106 30 30 PRO CB C 31.837 0.000 1 107 31 31 GLN H H 8.508 0.000 1 108 31 31 GLN CA C 56.344 0.000 1 109 31 31 GLN CB C 29.393 0.000 1 110 31 31 GLN N N 120.239 0.000 1 111 32 32 ARG H H 8.402 0.000 1 112 32 32 ARG CA C 54.179 0.000 1 113 32 32 ARG CB C 32.721 0.000 1 114 32 32 ARG N N 124.437 0.000 1 115 33 33 PRO CA C 63.274 0.000 1 116 33 33 PRO CB C 31.915 0.000 1 117 34 34 ILE H H 8.363 0.000 1 118 34 34 ILE CA C 61.484 0.000 1 119 34 34 ILE CB C 38.843 0.000 1 120 34 34 ILE N N 121.429 0.000 1 121 35 35 SER H H 8.458 0.000 1 122 35 35 SER CA C 58.254 0.000 1 123 35 35 SER CB C 64.024 0.000 1 124 35 35 SER N N 119.563 0.000 1 125 36 36 THR H H 8.258 0.000 1 126 36 36 THR CA C 62.029 0.000 1 127 36 36 THR CB C 69.784 0.000 1 128 36 36 THR N N 116.117 0.000 1 129 37 37 GLN H H 8.381 0.000 1 130 37 37 GLN CA C 55.724 0.000 1 131 37 37 GLN CB C 29.346 0.000 1 132 37 37 GLN N N 122.659 0.000 1 133 38 38 ARG H H 8.370 0.000 1 134 38 38 ARG CA C 56.658 0.000 1 135 38 38 ARG CB C 30.319 0.000 1 136 38 38 ARG N N 123.471 0.000 1 137 39 39 THR H H 8.233 0.000 1 138 39 39 THR CA C 61.873 0.000 1 139 39 39 THR CB C 69.900 0.000 1 140 39 39 THR N N 115.864 0.000 1 141 40 40 ALA H H 8.390 0.000 1 142 40 40 ALA CA C 52.299 0.000 1 143 40 40 ALA CB C 19.305 0.000 1 144 40 40 ALA N N 126.864 0.000 1 145 41 41 ALA H H 8.274 0.000 1 146 41 41 ALA CA C 52.065 0.000 1 147 41 41 ALA CB C 19.188 0.000 1 148 41 41 ALA N N 123.731 0.000 1 149 42 42 ALA H H 8.296 0.000 1 150 42 42 ALA CA C 50.257 0.000 1 151 42 42 ALA CB C 17.891 0.000 1 152 42 42 ALA N N 125.128 0.000 1 153 43 43 PRO CA C 63.080 0.000 1 154 43 43 PRO CB C 31.954 0.000 1 155 44 44 LYS H H 8.463 0.000 1 156 44 44 LYS CA C 56.230 0.000 1 157 44 44 LYS CB C 33.044 0.000 1 158 44 44 LYS N N 121.929 0.000 1 159 45 45 ALA H H 8.373 0.000 1 160 45 45 ALA CA C 52.299 0.000 1 161 45 45 ALA CB C 19.733 0.000 1 162 45 45 ALA N N 125.533 0.000 1 163 46 46 GLY H H 8.277 0.000 1 164 46 46 GLY CA C 44.228 0.000 1 165 46 46 GLY N N 108.648 0.000 1 166 47 47 PRO CA C 63.624 0.000 1 167 47 47 PRO CB C 31.954 0.000 1 168 48 48 GLY H H 8.581 0.000 1 169 48 48 GLY CA C 45.254 0.000 1 170 48 48 GLY N N 109.516 0.000 1 171 49 49 VAL H H 7.945 0.000 1 172 49 49 VAL CA C 62.379 0.000 1 173 49 49 VAL CB C 32.693 0.000 1 174 49 49 VAL N N 120.026 0.000 1 175 50 50 VAL H H 8.325 0.000 1 176 50 50 VAL CA C 62.184 0.000 1 177 50 50 VAL CB C 32.654 0.000 1 178 50 50 VAL N N 125.339 0.000 1 179 51 51 ARG H H 8.507 0.000 1 180 51 51 ARG CA C 55.851 0.000 1 181 51 51 ARG CB C 30.825 0.000 1 182 51 51 ARG N N 126.287 0.000 1 183 52 52 LYS H H 8.503 0.000 1 184 52 52 LYS CA C 56.327 0.000 1 185 52 52 LYS CB C 33.297 0.000 1 186 52 52 LYS N N 123.712 0.000 1 187 53 53 ASN H H 8.632 0.000 1 188 53 53 ASN CA C 51.438 0.000 1 189 53 53 ASN CB C 38.725 0.000 1 190 53 53 ASN N N 121.367 0.000 1 191 54 54 PRO CA C 63.800 0.000 1 192 54 54 PRO CB C 32.012 0.000 1 193 55 55 GLY H H 8.491 0.000 1 194 55 55 GLY CA C 45.235 0.000 1 195 55 55 GLY N N 109.095 0.000 1 196 56 56 VAL H H 8.028 0.000 1 197 56 56 VAL CA C 62.282 0.000 1 198 56 56 VAL CB C 32.674 0.000 1 199 56 56 VAL N N 118.957 0.000 1 200 57 57 GLY H H 8.628 0.000 1 201 57 57 GLY CA C 45.235 0.000 1 202 57 57 GLY N N 112.598 0.000 1 203 58 58 ASN H H 8.500 0.000 1 204 58 58 ASN CA C 53.330 0.000 1 205 58 58 ASN CB C 39.290 0.000 1 206 58 58 ASN N N 118.979 0.000 1 207 59 59 GLY H H 8.569 0.000 1 208 59 59 GLY CA C 45.507 0.000 1 209 59 59 GLY N N 109.724 0.000 1 210 60 60 ASP H H 8.331 0.000 1 211 60 60 ASP CA C 54.303 0.000 1 212 60 60 ASP CB C 41.353 0.000 1 213 60 60 ASP N N 120.990 0.000 1 214 61 61 ASP H H 8.003 0.000 1 215 61 61 ASP CA C 55.807 0.000 1 216 61 61 ASP CB C 41.801 0.000 1 217 61 61 ASP N N 125.541 0.000 1 stop_ save_ save_heteronucl_T1_relaxation_1 _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 800 _T1_coherence_type Iz _T1_value_units s-1 _Mol_system_component_name EB1linker-4D _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 5 THR N 1.132 0.034 2 6 ALA N 1.183 0.029 3 7 VAL N 1.124 0.003 4 8 ALA N 1.240 0.027 5 10 SER N 1.286 0.042 6 11 LEU N 1.380 0.024 7 12 VAL N 1.298 0.004 8 13 ALA N 1.300 0.010 9 15 ALA N 1.298 0.018 10 16 LEU N 1.296 0.017 11 17 ASN N 1.316 0.070 12 18 LYS N 1.327 0.040 13 20 LYS N 1.441 0.019 14 21 LYS N 1.404 0.067 15 23 LEU N 1.470 0.009 16 24 ASP N 1.380 0.006 17 26 ASP N 1.442 0.010 18 27 ASP N 1.488 0.005 19 28 ALA N 1.393 0.007 20 29 ALA N 1.333 0.004 21 31 GLN N 1.148 0.053 22 32 ARG N 1.474 0.025 23 34 ILE N 1.337 0.010 24 35 SER N 1.417 0.067 25 36 THR N 1.308 0.070 26 37 GLN N 1.472 0.063 27 39 THR N 1.302 0.062 28 40 ALA N 1.302 0.063 29 41 ALA N 1.186 0.043 30 42 ALA N 1.162 0.020 31 44 LYS N 1.275 0.032 32 45 ALA N 1.282 0.037 33 46 GLY N 1.111 0.030 34 48 GLY N 1.232 0.057 35 49 VAL N 1.334 0.009 36 50 VAL N 1.339 0.007 37 51 ARG N 1.452 0.034 38 52 LYS N 1.455 0.061 39 53 ASN N 1.408 0.070 40 55 GLY N 1.374 0.043 41 56 VAL N 1.304 0.003 42 57 GLY N 1.258 0.040 43 58 ASN N 1.164 0.078 44 59 GLY N 1.084 0.046 45 61 ASP N 0.782 0.006 stop_ save_ save_heteronucl_T2_relaxation_1 _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 800 _T2_coherence_type I(+,-) _T2_value_units s-1 _Mol_system_component_name EB1linker-4D _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 3 GLN N 1.873 0.034 . . 2 4 GLU N 1.582 0.018 . . 3 5 THR N 1.797 0.043 . . 4 6 ALA N 1.546 0.043 . . 5 7 VAL N 1.594 0.026 . . 6 8 ALA N 1.849 0.046 . . 7 10 SER N 2.414 0.041 . . 8 11 LEU N 2.068 0.047 . . 9 12 VAL N 2.048 0.054 . . 10 13 ALA N 2.221 0.097 . . 11 15 ALA N 2.409 0.035 . . 12 16 LEU N 2.265 0.041 . . 13 17 ASN N 2.615 0.054 . . 14 18 LYS N 2.510 0.051 . . 15 20 LYS N 2.426 0.064 . . 16 21 LYS N 3.056 0.114 . . 17 23 LEU N 2.895 0.086 . . 18 24 ASP N 2.326 0.055 . . 19 26 ASP N 2.923 0.058 . . 20 27 ASP N 2.938 0.045 . . 21 28 ALA N 2.637 0.046 . . 22 29 ALA N 2.221 0.038 . . 23 31 GLN N 1.787 0.053 . . 24 32 ARG N 2.640 0.049 . . 25 34 ILE N 2.355 0.042 . . 26 35 SER N 2.841 0.087 . . 27 36 THR N 2.336 0.025 . . 28 37 GLN N 3.122 0.135 . . 29 39 THR N 3.244 0.116 . . 30 40 ALA N 1.977 0.060 . . 31 41 ALA N 1.985 0.043 . . 32 42 ALA N 1.764 0.037 . . 33 44 LYS N 1.918 0.041 . . 34 45 ALA N 2.035 0.043 . . 35 46 GLY N 1.802 0.014 . . 36 48 GLY N 2.114 0.044 . . 37 49 VAL N 2.160 0.030 . . 38 50 VAL N 2.249 0.024 . . 39 51 ARG N 2.418 0.040 . . 40 52 LYS N 2.751 0.035 . . 41 53 ASN N 2.752 0.065 . . 42 55 GLY N 2.056 0.057 . . 43 56 VAL N 1.826 0.034 . . 44 57 GLY N 1.985 0.031 . . 45 58 ASN N 1.953 0.031 . . 46 59 GLY N 1.545 0.036 . . 47 61 ASP N 1.758 0.024 . . stop_ save_ save_heteronucl_NOEs_1 _Saveframe_category heteronuclear_NOE _Details . loop_ _Experiment_label '15N-(1H) NOE' stop_ loop_ _Sample_label . stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 800 _Mol_system_component_name EB1linker-4D _Atom_one_atom_name N _Atom_two_atom_name H _Heteronuclear_NOE_value_type 'peak integral' _NOE_reference_value 1.00 _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 3 GLN -0.794 0.000 4 GLU -0.565 0.000 5 THR -0.435 0.000 6 ALA -0.278 0.000 7 VAL -0.266 0.000 8 ALA -0.206 0.000 10 SER -0.066 0.000 11 LEU 0.009 0.000 12 VAL -0.075 0.000 13 ALA -0.097 0.000 15 ALA -0.067 0.000 16 LEU -0.001 0.000 17 ASN 0.000 0.000 18 LYS 0.056 0.000 20 LYS 0.084 0.000 21 LYS 0.070 0.000 23 LEU 0.116 0.000 24 ASP 0.069 0.000 26 ASP 0.135 0.000 27 ASP 0.115 0.000 28 ALA 0.116 0.000 29 ALA 0.106 0.000 31 GLN -0.219 0.000 32 ARG 0.162 0.000 34 ILE 0.045 0.000 35 SER 0.118 0.000 37 GLN 0.133 0.000 40 ALA 0.007 0.000 41 ALA -0.107 0.000 42 ALA -0.117 0.000 44 LYS -0.119 0.000 45 ALA -0.097 0.000 46 GLY -0.182 0.000 48 GLY -0.070 0.000 49 VAL 0.059 0.000 50 VAL -0.055 0.000 51 ARG 0.050 0.000 52 LYS 0.094 0.000 53 ASN 0.060 0.000 55 GLY -0.050 0.000 56 VAL -0.027 0.000 57 GLY -0.202 0.000 58 ASN -0.318 0.000 59 GLY -0.498 0.000 61 ASP -0.981 0.000 stop_ save_