data_50119
#######################
# Entry information #
#######################
save_entry_information
_Saveframe_category entry_information
_Entry_title
;
Intramolecular synergy enhances the microtubule end-binding affinity of EB1
;
_BMRB_accession_number 50119
_BMRB_flat_file_name bmr50119.str
_Entry_type original
_Submission_date 2019-12-09
_Accession_date 2019-12-09
_Entry_origination author
_NMR_STAR_version 2.1.1
_Experimental_method NMR
_Details .
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Xue Yi . .
2 Pan Ying . .
stop_
loop_
_Saveframe_category_type
_Saveframe_category_type_count
assigned_chemical_shifts 1
heteronucl_NOE 1
T1_relaxation 1
T2_relaxation 1
stop_
loop_
_Data_type
_Data_type_count
"1H chemical shifts" 50
"13C chemical shifts" 174
"15N chemical shifts" 48
"T1 relaxation values" 45
"T2 relaxation values" 47
stop_
loop_
_Revision_date
_Revision_keyword
_Revision_author
_Revision_detail
2020-02-19 original BMRB .
stop_
loop_
_Related_BMRB_accession_number
_Relationship
50120 'EB1 linker-4D'
stop_
_Original_release_date 2020-01-29
save_
#############################
# Citation for this entry #
#############################
save_citations_1
_Saveframe_category entry_citation
_Citation_full .
_Citation_title
;
Intramolecular synergy enhances the microtubule end-binding affinity of EB1
;
_Citation_status 'in preparation'
_Citation_type journal
_CAS_abstract_code .
_MEDLINE_UI_code .
_PubMed_ID ?
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Liang . . .
2 Song . . .
3 Zhang . . .
4 Pan . . .
5 He . . .
6 Wang . . .
7 Chen . . .
8 Guo . . .
9 Deng . . .
10 Xue Yi . .
11 Fang Ying . .
stop_
_Journal_abbreviation 'Not known'
_Journal_volume .
_Journal_issue .
_Journal_CSD .
_Book_chapter_title .
_Book_volume .
_Book_series .
_Book_ISBN .
_Conference_state_province .
_Conference_abstract_number .
_Page_first .
_Page_last .
_Year .
_Details .
save_
##################################
# Molecular system description #
##################################
save_assembly_1
_Saveframe_category molecular_system
_Mol_system_name EB1linker-wt
_Enzyme_commission_number .
loop_
_Mol_system_component_name
_Mol_label
EB1linker-wt $entity_1
stop_
_System_molecular_weight .
_System_physical_state native
_System_oligomer_state ?
_System_paramagnetic no
_System_thiol_state .
_Database_query_date .
_Details .
save_
########################
# Monomeric polymers #
########################
save_entity_1
_Saveframe_category monomeric_polymer
_Mol_type polymer
_Mol_polymer_class protein
_Name_common entity_1
_Molecular_mass .
_Mol_thiol_state 'not present'
_Details .
##############################
# Polymer residue sequence #
##############################
_Residue_count 61
_Mol_residue_sequence
;
QGQETAVAPSLVAPALNKPK
KPLTSSSAAPQRPISTQRTA
AAPKAGPGVVRKNPGVGNGD
D
;
loop_
_Residue_seq_code
_Residue_label
1 GLN 2 GLY 3 GLN 4 GLU 5 THR
6 ALA 7 VAL 8 ALA 9 PRO 10 SER
11 LEU 12 VAL 13 ALA 14 PRO 15 ALA
16 LEU 17 ASN 18 LYS 19 PRO 20 LYS
21 LYS 22 PRO 23 LEU 24 THR 25 SER
26 SER 27 SER 28 ALA 29 ALA 30 PRO
31 GLN 32 ARG 33 PRO 34 ILE 35 SER
36 THR 37 GLN 38 ARG 39 THR 40 ALA
41 ALA 42 ALA 43 PRO 44 LYS 45 ALA
46 GLY 47 PRO 48 GLY 49 VAL 50 VAL
51 ARG 52 LYS 53 ASN 54 PRO 55 GLY
56 VAL 57 GLY 58 ASN 59 GLY 60 ASP
61 ASP
stop_
_Sequence_homology_query_date .
_Sequence_homology_query_revised_last_date .
save_
####################
# Natural source #
####################
save_natural_source_1
_Saveframe_category natural_source
loop_
_Mol_label
_Organism_name_common
_NCBI_taxonomy_ID
_Superkingdom
_Kingdom
_Genus
_Species
$entity_1 'E. coli' 562 Bacteria . Escherichia coli
stop_
save_
#########################
# Experimental source #
#########################
save_experimental_source_1
_Saveframe_category experimental_source
loop_
_Mol_label
_Production_method
_Host_organism_name_common
_Genus
_Species
_Strain
_Vector_type
_Vector_name
$entity_1 'recombinant technology' . Escherichia coli . plasmid pet28a-SUMO
stop_
save_
#####################################
# Sample contents and methodology #
#####################################
########################
# Sample description #
########################
save_sample_1
_Saveframe_category sample
_Sample_type solution
_Details .
loop_
_Mol_label
_Concentration_value
_Concentration_value_units
_Isotopic_labeling
$entity_1 0.5 mM '[U-99% 15N]'
'sodium chloride' 50 mM 'natural abundance'
'sodium phosphate' 20 mM 'natural abundance'
H2O 90 % 'natural abundance'
D2O 10 % 'natural abundance'
DSS 20 uM 'natural abundance'
'sodium azide' 0.01 % 'natural abundance'
stop_
save_
save_sample_2
_Saveframe_category sample
_Sample_type solution
_Details .
loop_
_Mol_label
_Concentration_value
_Concentration_value_units
_Isotopic_labeling
$entity_1 0.5 mM '[U-99% 13C; U-99% 15N]'
'sodium chloride' 50 mM 'natural abundance'
'sodium phosphate' 20 mM 'natural abundance'
H2O 90 % 'natural abundance'
D2O 10 % 'natural abundance'
DSS 20 uM 'natural abundance'
'sodium azide' 0.01 % 'natural abundance'
stop_
save_
############################
# Computer software used #
############################
save_software_1
_Saveframe_category software
_Name TOPSPIN
_Version .
loop_
_Vendor
_Address
_Electronic_address
'Bruker Biospin' . .
stop_
loop_
_Task
collection
stop_
_Details .
save_
save_software_2
_Saveframe_category software
_Name NMRPipe
_Version .
loop_
_Vendor
_Address
_Electronic_address
'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . .
stop_
loop_
_Task
'data analysis'
processing
stop_
_Details .
save_
save_software_3
_Saveframe_category software
_Name SPARKY
_Version .
loop_
_Vendor
_Address
_Electronic_address
Goddard . .
stop_
loop_
_Task
'chemical shift assignment'
'peak picking'
stop_
_Details .
save_
#########################
# Experimental detail #
#########################
##################################
# NMR Spectrometer definitions #
##################################
save_NMR_spectrometer_1
_Saveframe_category NMR_spectrometer
_Manufacturer Bruker
_Model 'AVANCE III HD'
_Field_strength 800
_Details 'with cryoprobe'
save_
#############################
# NMR applied experiments #
#############################
save_2D_1H-15N_HSQC_1
_Saveframe_category NMR_applied_experiment
_Experiment_name '2D 1H-15N HSQC'
_Sample_label $sample_2
save_
save_3D_HNCACB_2
_Saveframe_category NMR_applied_experiment
_Experiment_name '3D HNCACB'
_Sample_label $sample_2
save_
save_3D_CBCA(CO)NH_3
_Saveframe_category NMR_applied_experiment
_Experiment_name '3D CBCA(CO)NH'
_Sample_label $sample_2
save_
save_3D_HNCA_4
_Saveframe_category NMR_applied_experiment
_Experiment_name '3D HNCA'
_Sample_label $sample_2
save_
save_3D_HN(CO)CA_5
_Saveframe_category NMR_applied_experiment
_Experiment_name '3D HN(CO)CA'
_Sample_label $sample_2
save_
save_15N-(1H)_NOE_6
_Saveframe_category NMR_applied_experiment
_Experiment_name '15N-(1H) NOE'
_Sample_label $sample_1
save_
save_15N_R1_7
_Saveframe_category NMR_applied_experiment
_Experiment_name '15N R1'
_Sample_label $sample_1
save_
save_15N_R2_8
_Saveframe_category NMR_applied_experiment
_Experiment_name '15N R2'
_Sample_label $sample_1
save_
#######################
# Sample conditions #
#######################
save_sample_conditions_1
_Saveframe_category sample_conditions
_Details .
loop_
_Variable_type
_Variable_value
_Variable_value_error
_Variable_value_units
'ionic strength' 0.17 . M
pH 6.0 . pH
pressure 1 . atm
temperature 298 . K
stop_
save_
####################
# NMR parameters #
####################
##############################
# Assigned chemical shifts #
##############################
################################
# Chemical shift referencing #
################################
save_chem_shift_reference_1
_Saveframe_category chemical_shift_reference
_Details .
loop_
_Mol_common_name
_Atom_type
_Atom_isotope_number
_Atom_group
_Chem_shift_units
_Chem_shift_value
_Reference_method
_Reference_type
_External_reference_sample_geometry
_External_reference_location
_External_reference_axis
_Indirect_shift_ratio
DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530
DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000
DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118
stop_
save_
###################################
# Assigned chemical shift lists #
###################################
###################################################################
# Chemical Shift Ambiguity Index Value Definitions #
# #
# The values other than 1 are used for those atoms with different #
# chemical shifts that cannot be assigned to stereospecific atoms #
# or to specific residues or chains. #
# #
# Index Value Definition #
# #
# 1 Unique (including isolated methyl protons, #
# geminal atoms, and geminal methyl #
# groups with identical chemical shifts) #
# (e.g. ILE HD11, HD12, HD13 protons) #
# 2 Ambiguity of geminal atoms or geminal methyl #
# proton groups (e.g. ASP HB2 and HB3 #
# protons, LEU CD1 and CD2 carbons, or #
# LEU HD11, HD12, HD13 and HD21, HD22, #
# HD23 methyl protons) #
# 3 Aromatic atoms on opposite sides of #
# symmetrical rings (e.g. TYR HE1 and HE2 #
# protons) #
# 4 Intraresidue ambiguities (e.g. LYS HG and #
# HD protons or TRP HZ2 and HZ3 protons) #
# 5 Interresidue ambiguities (LYS 12 vs. LYS 27) #
# 6 Intermolecular ambiguities (e.g. ASP 31 CA #
# in monomer 1 and ASP 31 CA in monomer 2 #
# of an asymmetrical homodimer, duplex #
# DNA assignments, or other assignments #
# that may apply to atoms in one or more #
# molecule in the molecular assembly) #
# 9 Ambiguous, specific ambiguity not defined #
# #
###################################################################
save_assigned_chemical_shifts_1
_Saveframe_category assigned_chemical_shifts
_Details .
loop_
_Experiment_label
'2D 1H-15N HSQC'
'3D HNCACB'
'3D CBCA(CO)NH'
'3D HNCA'
'3D HN(CO)CA'
stop_
loop_
_Sample_label
$sample_2
stop_
_Sample_conditions_label $sample_conditions_1
_Chem_shift_reference_set_label $chem_shift_reference_1
_Mol_system_component_name EB1linker-wt
_Text_data_format .
_Text_data .
loop_
_Atom_shift_assign_ID
_Residue_author_seq_code
_Residue_seq_code
_Residue_label
_Atom_name
_Atom_type
_Chem_shift_value
_Chem_shift_value_error
_Chem_shift_ambiguity_code
1 1 1 GLN C C 178.462 0.000 1
2 1 1 GLN CA C 59.914 0.000 1
3 1 1 GLN CB C 27.811 0.000 1
4 2 2 GLY C C 174.139 0.000 1
5 2 2 GLY CA C 45.082 0.000 1
6 3 3 GLN H H 8.561 0.000 1
7 3 3 GLN C C 176.231 0.000 1
8 3 3 GLN CA C 55.935 0.000 1
9 3 3 GLN CB C 29.209 0.000 1
10 3 3 GLN N N 120.636 0.000 1
11 4 4 GLU H H 8.732 0.000 1
12 4 4 GLU C C 176.639 0.000 1
13 4 4 GLU CA C 56.782 0.000 1
14 4 4 GLU CB C 30.180 0.000 1
15 4 4 GLU N N 122.702 0.000 1
16 5 5 THR H H 8.251 0.000 1
17 5 5 THR C C 174.172 0.000 1
18 5 5 THR CA C 62.009 0.000 1
19 5 5 THR CB C 70.270 0.000 1
20 5 5 THR N N 115.677 0.000 1
21 6 6 ALA H H 8.376 0.000 1
22 6 6 ALA C C 177.406 0.000 1
23 6 6 ALA CA C 52.430 0.000 1
24 6 6 ALA CB C 19.275 0.000 1
25 6 6 ALA N N 127.261 0.000 1
26 7 7 VAL H H 8.154 0.000 1
27 7 7 VAL C C 175.621 0.000 1
28 7 7 VAL CA C 62.079 0.000 1
29 7 7 VAL CB C 32.771 0.000 1
30 7 7 VAL N N 119.978 0.000 1
31 8 8 ALA H H 8.437 0.000 1
32 8 8 ALA C C 173.007 0.000 1
33 8 8 ALA CA C 50.519 0.000 1
34 8 8 ALA CB C 18.190 0.000 1
35 8 8 ALA N N 129.729 0.000 1
36 9 9 PRO C C 177.008 0.000 1
37 9 9 PRO CA C 63.414 0.000 1
38 9 9 PRO CB C 32.051 0.000 1
39 10 10 SER H H 8.366 0.000 1
40 10 10 SER C C 173.653 0.000 1
41 10 10 SER CA C 58.260 0.000 1
42 10 10 SER CB C 63.887 0.000 1
43 10 10 SER N N 115.543 0.000 1
44 11 11 LEU H H 8.348 0.000 1
45 11 11 LEU C C 177.090 0.000 1
46 11 11 LEU CA C 55.171 0.000 1
47 11 11 LEU CB C 42.415 0.000 1
48 11 11 LEU N N 124.519 0.000 1
49 12 12 VAL H H 120.980 0.000 1
50 12 12 VAL C C 175.451 0.000 1
51 12 12 VAL CA C 62.150 0.000 1
52 12 12 VAL CB C 32.448 0.000 1
53 12 12 VAL N N 8.044 0.000 1
54 13 13 ALA H H 8.383 0.000 1
55 13 13 ALA C C 172.862 0.000 1
56 13 13 ALA CA C 50.474 0.000 1
57 13 13 ALA CB C 18.190 0.000 1
58 13 13 ALA N N 129.634 0.000 1
59 14 14 PRO C C 176.721 0.000 1
60 14 14 PRO CA C 63.338 0.000 1
61 14 14 PRO CB C 32.018 0.000 1
62 15 15 ALA H H 8.383 0.000 1
63 15 15 ALA C C 177.760 0.000 1
64 15 15 ALA CA C 52.371 0.000 1
65 15 15 ALA CB C 19.069 0.000 1
66 15 15 ALA N N 123.927 0.000 1
67 16 16 LEU H H 8.238 0.000 1
68 16 16 LEU C C 177.087 0.000 1
69 16 16 LEU CA C 54.955 0.000 1
70 16 16 LEU CB C 42.329 0.000 1
71 16 16 LEU N N 121.315 0.000 1
72 17 17 ASN H H 119.448 0.000 1
73 17 17 ASN C C 174.635 0.000 1
74 17 17 ASN CA C 52.964 0.000 1
75 17 17 ASN CB C 38.822 0.000 1
76 18 18 LYS H H 8.212 0.000 1
77 18 18 LYS C C 174.281 0.000 1
78 18 18 LYS CA C 54.404 0.000 1
79 18 18 LYS CB C 32.445 0.000 1
80 18 18 LYS N N 122.882 0.000 1
81 19 19 PRO C C 176.859 0.000 1
82 19 19 PRO CA C 63.224 0.000 1
83 19 19 PRO CB C 32.205 0.000 1
84 20 20 LYS H H 8.524 0.000 1
85 20 20 LYS C C 176.508 0.000 1
86 20 20 LYS CA C 56.340 0.000 1
87 20 20 LYS CB C 33.041 0.000 1
88 20 20 LYS N N 122.377 0.000 1
89 21 21 LYS H H 8.352 0.000 1
90 21 21 LYS C C 174.401 0.000 1
91 21 21 LYS CA C 54.327 0.000 1
92 21 21 LYS CB C 32.731 0.000 1
93 21 21 LYS N N 123.954 0.000 1
94 22 22 PRO C C 176.846 0.000 1
95 22 22 PRO CA C 63.062 0.000 1
96 22 22 PRO CB C 32.043 0.000 1
97 23 23 LEU H H 8.509 0.000 1
98 23 23 LEU C C 177.837 0.000 1
99 23 23 LEU CA C 55.220 0.000 1
100 23 23 LEU CB C 42.361 0.000 1
101 23 23 LEU N N 122.913 0.000 1
102 24 24 THR H H 8.177 0.000 1
103 24 24 THR C C 174.528 0.000 1
104 24 24 THR CA C 61.623 0.000 1
105 24 24 THR CB C 70.311 0.000 1
106 24 24 THR N N 114.587 0.000 1
107 25 25 SER H H 8.420 0.000 1
108 25 25 SER C C 174.718 0.000 1
109 25 25 SER CA C 58.231 0.000 1
110 25 25 SER CB C 64.097 0.000 1
111 25 25 SER N N 117.984 0.000 1
112 26 26 SER H H 8.491 0.000 1
113 26 26 SER C C 174.625 0.000 1
114 26 26 SER CA C 58.527 0.000 1
115 26 26 SER CB C 64.034 0.000 1
116 26 26 SER N N 118.153 0.000 1
117 27 27 SER H H 8.384 0.000 1
118 27 27 SER C C 174.060 0.000 1
119 27 27 SER CB C 64.113 0.000 1
120 27 27 SER N N 117.984 0.000 1
121 28 28 ALA H H 8.265 0.000 1
122 28 28 ALA CA C 52.319 0.000 1
123 28 28 ALA CB C 19.287 0.000 1
124 28 28 ALA N N 125.996 0.000 1
125 29 29 ALA H H 8.261 0.000 1
126 29 29 ALA C C 175.515 0.000 1
127 29 29 ALA CA C 50.498 0.000 1
128 29 29 ALA CB C 18.100 0.000 1
129 29 29 ALA N N 125.007 0.000 1
130 30 30 PRO C C 176.942 0.000 1
131 30 30 PRO CA C 63.151 0.000 1
132 30 30 PRO CB C 32.010 0.000 1
133 31 31 GLN H H 8.546 0.000 1
134 31 31 GLN C C 175.845 0.000 1
135 31 31 GLN CA C 55.609 0.000 1
136 31 31 GLN CB C 29.547 0.000 1
137 31 31 GLN N N 120.984 0.000 1
138 32 32 ARG H H 8.431 0.000 1
139 32 32 ARG C C 174.153 0.000 1
140 32 32 ARG CA C 56.055 0.000 1
141 32 32 ARG CB C 30.058 0.000 1
142 32 32 ARG N N 123.755 0.000 1
143 33 33 PRO C C 176.840 0.000 1
144 33 33 PRO CA C 63.128 0.000 1
145 33 33 PRO CB C 31.965 0.000 1
146 34 34 ILE H H 8.371 0.000 1
147 34 34 ILE C C 176.597 0.000 1
148 34 34 ILE CA C 61.423 0.000 1
149 34 34 ILE CB C 38.851 0.000 1
150 34 34 ILE N N 121.522 0.000 1
151 35 35 SER H H 8.463 0.000 1
152 35 35 SER C C 174.904 0.000 1
153 35 35 SER CA C 58.119 0.000 1
154 35 35 SER CB C 64.032 0.000 1
155 35 35 SER N N 119.691 0.000 1
156 36 36 THR H H 8.282 0.000 1
157 36 36 THR C C 174.573 0.000 1
158 36 36 THR CA C 62.178 0.000 1
159 36 36 THR CB C 69.949 0.000 1
160 36 36 THR N N 116.259 0.000 1
161 37 37 GLN H H 8.392 0.000 1
162 37 37 GLN C C 175.949 0.000 1
163 37 37 GLN CA C 56.100 0.000 1
164 37 37 GLN CB C 29.373 0.000 1
165 37 37 GLN N N 122.780 0.000 1
166 38 38 ARG H H 8.732 0.000 1
167 38 38 ARG C C 176.649 0.000 1
168 38 38 ARG CA C 56.764 0.000 1
169 38 38 ARG CB C 30.151 0.000 1
170 38 38 ARG N N 122.641 0.000 1
171 39 39 THR H H 8.245 0.000 1
172 39 39 THR C C 174.164 0.000 1
173 39 39 THR CA C 62.025 0.000 1
174 39 39 THR CB C 70.192 0.000 1
175 39 39 THR N N 116.003 0.000 1
176 40 40 ALA H H 8.396 0.000 1
177 40 40 ALA C C 177.174 0.000 1
178 40 40 ALA CA C 52.439 0.000 1
179 40 40 ALA CB C 19.278 0.000 1
180 40 40 ALA N N 126.918 0.000 1
181 41 41 ALA H H 8.278 0.000 1
182 41 41 ALA C C 177.069 0.000 1
183 41 41 ALA CA C 52.130 0.000 1
184 41 41 ALA CB C 19.260 0.000 1
185 41 41 ALA N N 123.771 0.000 1
186 42 42 ALA H H 8.301 0.000 1
187 42 42 ALA C C 176.278 0.000 1
188 42 42 ALA CA C 50.514 0.000 1
189 42 42 ALA CB C 18.190 0.000 1
190 42 42 ALA N N 125.165 0.000 1
191 43 43 PRO C C 176.929 0.000 1
192 43 43 PRO CA C 63.063 0.000 1
193 43 43 PRO CB C 32.026 0.000 1
194 44 44 LYS H H 8.463 0.000 1
195 44 44 LYS C C 176.231 0.000 1
196 44 44 LYS CA C 56.428 0.000 1
197 44 44 LYS CB C 32.978 0.000 1
198 44 44 LYS N N 121.949 0.000 1
199 45 45 ALA H H 8.375 0.000 1
200 45 45 ALA C C 177.667 0.000 1
201 45 45 ALA CA C 52.321 0.000 1
202 45 45 ALA CB C 19.845 0.000 1
203 45 45 ALA N N 125.566 0.000 1
204 46 46 GLY H H 8.278 0.000 1
205 46 46 GLY C C 171.898 0.000 1
206 46 46 GLY CA C 44.609 0.000 1
207 46 46 GLY N N 108.671 0.000 1
208 47 47 PRO C C 177.824 0.000 1
209 47 47 PRO CA C 63.733 0.000 1
210 47 47 PRO CB C 32.084 0.000 1
211 48 48 GLY H H 8.579 0.000 1
212 48 48 GLY C C 173.995 0.000 1
213 48 48 GLY CA C 45.275 0.000 1
214 48 48 GLY N N 109.548 0.000 1
215 49 49 VAL H H 7.945 0.000 1
216 49 49 VAL C C 176.125 0.000 1
217 49 49 VAL CA C 62.503 0.000 1
218 49 49 VAL CB C 32.691 0.000 1
219 49 49 VAL N N 120.048 0.000 1
220 50 50 VAL H H 8.327 0.000 1
221 50 50 VAL C C 175.935 0.000 1
222 50 50 VAL CA C 62.482 0.000 1
223 50 50 VAL CB C 32.646 0.000 1
224 50 50 VAL N N 125.370 0.000 1
225 51 51 ARG H H 8.509 0.000 1
226 51 51 ARG C C 175.882 0.000 1
227 51 51 ARG CA C 55.561 0.000 1
228 51 51 ARG CB C 30.983 0.000 1
229 51 51 ARG N N 126.316 0.000 1
230 52 52 LYS H H 8.507 0.000 1
231 52 52 LYS C C 176.010 0.000 1
232 52 52 LYS CA C 56.533 0.000 1
233 52 52 LYS CB C 33.127 0.000 1
234 52 52 LYS N N 123.742 0.000 1
235 53 53 ASN H H 121.370 0.000 1
236 53 53 ASN C C 173.349 0.000 1
237 53 53 ASN CA C 51.442 0.000 1
238 53 53 ASN CB C 38.829 0.000 1
239 54 54 PRO C C 177.398 0.000 1
240 54 54 PRO CA C 63.827 0.000 1
241 54 54 PRO CB C 32.098 0.000 1
242 55 55 GLY H H 8.493 0.000 1
243 55 55 GLY C C 174.143 0.000 1
244 55 55 GLY CA C 45.171 0.000 1
245 55 55 GLY N N 109.125 0.000 1
246 56 56 VAL H H 8.030 0.000 1
247 56 56 VAL C C 176.781 0.000 1
248 56 56 VAL CA C 62.502 0.000 1
249 56 56 VAL CB C 32.684 0.000 1
250 56 56 VAL N N 118.972 0.000 1
251 57 57 GLY H H 8.629 0.000 1
252 57 57 GLY C C 174.107 0.000 1
253 57 57 GLY CA C 45.171 0.000 1
254 57 57 GLY N N 112.616 0.000 1
255 58 58 ASN H H 8.502 0.000 1
256 58 58 ASN C C 175.798 0.000 1
257 58 58 ASN CA C 53.131 0.000 1
258 58 58 ASN CB C 39.120 0.000 1
259 58 58 ASN N N 118.990 0.000 1
260 59 59 GLY H H 8.571 0.000 1
261 59 59 GLY C C 173.961 0.000 1
262 59 59 GLY CA C 45.417 0.000 1
263 59 59 GLY N N 109.642 0.000 1
264 60 60 ASP H H 8.330 0.000 1
265 60 60 ASP C C 175.196 0.000 1
266 60 60 ASP CB C 41.258 0.000 1
267 60 60 ASP N N 120.928 0.000 1
268 61 61 ASP H H 8.003 0.000 1
269 61 61 ASP C C 175.204 0.000 1
270 61 61 ASP CA C 55.966 0.000 1
271 61 61 ASP CB C 42.147 0.000 1
272 61 61 ASP N N 125.539 0.000 1
stop_
save_
save_heteronucl_T1_relaxation_1
_Saveframe_category T1_relaxation
_Details .
loop_
_Sample_label
$sample_1
stop_
_Sample_conditions_label $sample_conditions_1
_Spectrometer_frequency_1H 800
_T1_coherence_type Iz
_T1_value_units s-1
_Mol_system_component_name EB1linker-wt
_Text_data_format .
_Text_data .
loop_
_T1_ID
_Residue_seq_code
_Residue_label
_Atom_name
_T1_value
_T1_value_error
1 5 THR N 1.180 0.018
2 6 ALA N 1.217 0.013
3 7 VAL N 1.145 0.003
4 8 ALA N 1.246 0.003
5 10 SER N 1.291 0.022
6 11 LEU N 1.370 0.042
7 12 VAL N 1.289 0.004
8 13 ALA N 1.303 0.003
9 15 ALA N 1.296 0.017
10 16 LEU N 1.305 0.009
11 17 ASN N 1.306 0.063
12 18 LYS N 1.330 0.044
13 20 LYS N 1.422 0.024
14 21 LYS N 1.441 0.011
15 23 LEU N 1.385 0.040
16 24 THR N 1.323 0.040
17 26 SER N 1.146 0.015
18 27 SER N 1.149 0.037
19 28 ALA N 1.213 0.042
20 29 ALA N 1.169 0.017
21 31 GLN N 1.292 0.024
22 32 ARG N 1.367 0.027
23 34 ILE N 1.390 0.003
24 35 SER N 1.389 0.028
25 36 THR N 1.306 0.056
26 37 GLN N 1.418 0.040
27 39 THR N 1.309 0.057
28 40 ALA N 1.295 0.020
29 41 ALA N 1.198 0.030
30 42 ALA N 1.169 0.010
31 44 LYS N 1.287 0.024
32 45 ALA N 1.279 0.029
33 46 GLY N 1.116 0.018
34 48 GLY N 1.227 0.048
35 49 VAL N 1.338 0.009
36 50 VAL N 1.338 0.003
37 51 ARG N 1.446 0.029
38 52 LYS N 1.463 0.037
39 53 ASN N 1.399 0.037
40 55 GLY N 1.374 0.030
41 56 VAL N 1.322 0.012
42 57 GLY N 1.275 0.026
43 58 ASN N 1.201 0.041
44 59 GLY N 1.103 0.039
45 61 ASP N 0.836 0.012
stop_
save_
save_heteronucl_T2_relaxation_1
_Saveframe_category T2_relaxation
_Details .
loop_
_Sample_label
$sample_1
stop_
_Sample_conditions_label $sample_conditions_1
_Spectrometer_frequency_1H 800
_T2_coherence_type I(+,-)
_T2_value_units s-1
_Mol_system_component_name EB1linker-wt
_Text_data_format .
_Text_data .
loop_
_T2_ID
_Residue_seq_code
_Residue_label
_Atom_name
_T2_value
_T2_value_error
_Rex_value
_Rex_error
1 3 GLN N 1.636 0.013 . .
2 4 GLU N 1.449 0.014 . .
3 5 THR N 1.710 0.019 . .
4 6 ALA N 1.524 0.036 . .
5 7 VAL N 1.660 0.025 . .
6 8 ALA N 1.777 0.050 . .
7 10 SER N 2.236 0.028 . .
8 11 LEU N 2.161 0.035 . .
9 12 VAL N 2.015 0.035 . .
10 13 ALA N 2.098 0.061 . .
11 15 ALA N 2.286 0.046 . .
12 16 LEU N 2.235 0.039 . .
13 17 ASN N 2.442 0.044 . .
14 18 LYS N 2.408 0.043 . .
15 20 LYS N 2.558 0.082 . .
16 21 LYS N 2.572 0.040 . .
17 23 LEU N 2.519 0.046 . .
18 24 THR N 2.443 0.051 . .
19 26 SER N 2.949 0.135 . .
20 27 SER N 2.514 0.086 . .
21 28 ALA N 2.056 0.033 . .
22 29 ALA N 2.088 0.021 . .
23 31 GLN N 2.234 0.046 . .
24 32 ARG N 2.525 0.040 . .
25 34 ILE N 2.447 0.035 . .
26 35 SER N 2.888 0.056 . .
27 36 THR N 2.979 0.051 . .
28 37 GLN N 2.533 0.094 . .
29 39 THR N 2.659 0.048 . .
30 40 ALA N 2.089 0.071 . .
31 41 ALA N 2.003 0.034 . .
32 42 ALA N 1.951 0.039 . .
33 44 LYS N 1.907 0.047 . .
34 45 ALA N 1.934 0.034 . .
35 46 GLY N 1.732 0.015 . .
36 48 GLY N 1.860 0.049 . .
37 49 VAL N 2.037 0.019 . .
38 50 VAL N 2.130 0.017 . .
39 51 ARG N 2.181 0.031 . .
40 52 LYS N 2.420 0.038 . .
41 53 ASN N 2.417 0.028 . .
42 55 GLY N 1.838 0.057 . .
43 56 VAL N 1.675 0.029 . .
44 57 GLY N 1.790 0.035 . .
45 58 ASN N 1.590 0.025 . .
46 59 GLY N 1.221 0.034 . .
47 61 ASP N 0.968 0.009 . .
stop_
save_
save_heteronucl_NOEs_1
_Saveframe_category heteronuclear_NOE
_Details .
loop_
_Experiment_label
'15N-(1H) NOE'
stop_
loop_
_Sample_label
.
stop_
_Sample_conditions_label $sample_conditions_1
_Spectrometer_frequency_1H 800
_Mol_system_component_name EB1linker-wt
_Atom_one_atom_name N
_Atom_two_atom_name H
_Heteronuclear_NOE_value_type 'peak integral'
_NOE_reference_value 1.00
_NOE_reference_description .
_Text_data_format .
_Text_data .
loop_
_Residue_seq_code
_Residue_label
_Heteronuclear_NOE_value
_Heteronuclear_NOE_value_error
3 GLN -0.817 0.000
5 THR -0.431 0.000
6 ALA -0.303 0.000
7 VAL -0.235 0.000
8 ALA -0.159 0.000
10 SER -0.058 0.000
11 LEU 0.008 0.000
12 VAL -0.068 0.000
13 ALA -0.106 0.000
15 ALA -0.093 0.000
16 LEU 0.006 0.000
17 ASN -0.023 0.000
18 LYS 0.033 0.000
20 LYS 0.059 0.000
21 LYS 0.106 0.000
23 LEU 0.024 0.000
24 THR -0.000 0.000
26 SER -0.119 0.000
27 SER -0.112 0.000
28 ALA -0.121 0.000
29 ALA -0.128 0.000
31 GLN -0.136 0.000
32 ARG 0.030 0.000
34 ILE 0.033 0.000
35 SER 0.051 0.000
37 GLN 0.081 0.000
40 ALA -0.019 0.000
41 ALA -0.093 0.000
42 ALA -0.077 0.000
44 LYS -0.156 0.000
45 ALA -0.134 0.000
46 GLY -0.212 0.000
48 GLY -0.092 0.000
49 VAL 0.039 0.000
50 VAL -0.125 0.000
51 ARG 0.036 0.000
52 LYS 0.081 0.000
53 ASN 0.054 0.000
55 GLY -0.107 0.000
56 VAL -0.030 0.000
57 GLY -0.195 0.000
58 ASN -0.295 0.000
59 GLY -0.496 0.000
61 ASP -0.908 0.000
stop_
save_