data_50105 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Nipah virus phosphoprotein residues 588-650 ; _BMRB_accession_number 50105 _BMRB_flat_file_name bmr50105.str _Entry_type original _Submission_date 2019-11-29 _Accession_date 2019-11-29 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jensen Malene . . 2 Blackledge Martin . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 57 "13C chemical shifts" 183 "15N chemical shifts" 58 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-05-29 update BMRB 'update entry citation' 2020-01-10 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 50098 'Nipah virus phosphoprotein residues 1-100' 50099 'Nipah virus phosphoprotein residues 91-190' 50100 'Nipah virus phosphoprotein residues 173-240' 50101 'Nipah virus phosphoprotein residues 223-319' 50102 'Nipah virus phosphoprotein residues 299-401' 50103 'Nipah virus phosphoprotein residues 387-479' stop_ _Original_release_date 2019-12-02 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural Description of the Nipah Virus Phosphoprotein and Its Interaction With STAT1 ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 32348724 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jensen 'Malene Ringkjybing' R. . 2 Yabukarski Filip . . 3 Communie Guillaume . . 4 Condamine Eric . . 5 Mas Caroline . . 6 Volchkova Valentina . . 7 Tarbouriech Nicolas . . 8 Bourhis Jean-Marie M. . 9 Volchkov Viktor . . 10 Blackledge Martin . . 11 Jamin Marc . . stop_ _Journal_abbreviation 'Biophys. J.' _Journal_name_full 'Biophysical journal' _Journal_volume 118 _Journal_issue 10 _Journal_ISSN 1542-0086 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2470 _Page_last 2488 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly_1 _Saveframe_category molecular_system _Mol_system_name 'Nipah virus phosphoprotein residues 588-650' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Nipah virus phosphoprotein' $entity_1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 72 _Mol_residue_sequence ; MGKNNPELKPVIGRDILEQQ SLFSFDNVKNFRDGSLTNEP YGAAVQLREDLILPELNFEE TNASLEHHHHHH ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 587 MET 2 588 GLY 3 589 LYS 4 590 ASN 5 591 ASN 6 592 PRO 7 593 GLU 8 594 LEU 9 595 LYS 10 596 PRO 11 597 VAL 12 598 ILE 13 599 GLY 14 600 ARG 15 601 ASP 16 602 ILE 17 603 LEU 18 604 GLU 19 605 GLN 20 606 GLN 21 607 SER 22 608 LEU 23 609 PHE 24 610 SER 25 611 PHE 26 612 ASP 27 613 ASN 28 614 VAL 29 615 LYS 30 616 ASN 31 617 PHE 32 618 ARG 33 619 ASP 34 620 GLY 35 621 SER 36 622 LEU 37 623 THR 38 624 ASN 39 625 GLU 40 626 PRO 41 627 TYR 42 628 GLY 43 629 ALA 44 630 ALA 45 631 VAL 46 632 GLN 47 633 LEU 48 634 ARG 49 635 GLU 50 636 ASP 51 637 LEU 52 638 ILE 53 639 LEU 54 640 PRO 55 641 GLU 56 642 LEU 57 643 ASN 58 644 PHE 59 645 GLU 60 646 GLU 61 647 THR 62 648 ASN 63 649 ALA 64 650 SER 65 651 LEU 66 652 GLU 67 653 HIS 68 654 HIS 69 655 HIS 70 656 HIS 71 657 HIS 72 658 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source_1 _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 'Nipah virus' 121791 Virus . henipavirus 'Nipah virus' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . Escherichia coli . plasmid pET28 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM '[U-13C; U-15N]' 'Bis-Tris ph 6.0' 20 mM 'natural abundance' NaCl 150 mM 'natural abundance' Arginine 50 mM 'natural abundance' glutamate 50 mM 'natural abundance' TCEP 0.5 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Agilent _Model Unity _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCACO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACO' _Sample_label $sample_1 save_ save_3D_HNCOCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCOCA' _Sample_label $sample_1 save_ save_3D_HNCA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCOCACB_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCOCACB' _Sample_label $sample_1 save_ save_3D_HNCACB_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 150 . mM pH 6.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 4.774 internal indirect . . . 0.251449530 water H 1 protons ppm 4.774 internal direct . . . 1 water N 15 protons ppm 4.774 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCO' '3D HNCACO' '3D HNCOCA' '3D HNCA' '3D HNCOCACB' '3D HNCACB' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name 'Nipah virus phosphoprotein' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 589 3 LYS C C 176.354 . 1 2 589 3 LYS CA C 56.823 . 1 3 589 3 LYS CB C 33.150 . 1 4 590 4 ASN H H 8.588 . 1 5 590 4 ASN C C 174.462 . 1 6 590 4 ASN CA C 53.261 . 1 7 590 4 ASN CB C 38.993 . 1 8 590 4 ASN N N 119.417 . 1 9 591 5 ASN H H 8.376 . 1 10 591 5 ASN C C 173.316 . 1 11 591 5 ASN CA C 51.469 . 1 12 591 5 ASN CB C 38.822 . 1 13 591 5 ASN N N 120.013 . 1 14 592 6 PRO C C 176.851 . 1 15 592 6 PRO CA C 63.597 . 1 16 592 6 PRO CB C 32.154 . 1 17 592 6 PRO N N 136.903 . 1 18 593 7 GLU H H 8.482 . 1 19 593 7 GLU C C 176.426 . 1 20 593 7 GLU CA C 56.647 . 1 21 593 7 GLU CB C 30.082 . 1 22 593 7 GLU N N 120.297 . 1 23 594 8 LEU H H 8.090 . 1 24 594 8 LEU C C 176.855 . 1 25 594 8 LEU CA C 54.937 . 1 26 594 8 LEU CB C 42.294 . 1 27 594 8 LEU N N 123.277 . 1 28 595 9 LYS H H 8.241 . 1 29 595 9 LYS C C 174.288 . 1 30 595 9 LYS CA C 54.092 . 1 31 595 9 LYS CB C 32.495 . 1 32 595 9 LYS N N 123.801 . 1 33 596 10 PRO C C 176.712 . 1 34 596 10 PRO CA C 63.084 . 1 35 596 10 PRO CB C 32.199 . 1 36 597 11 VAL H H 8.334 . 1 37 597 11 VAL C C 176.252 . 1 38 597 11 VAL CA C 62.457 . 1 39 597 11 VAL CB C 32.771 . 1 40 597 11 VAL N N 121.419 . 1 41 598 12 ILE H H 8.261 . 1 42 598 12 ILE C C 176.566 . 1 43 598 12 ILE CA C 61.067 . 1 44 598 12 ILE CB C 38.694 . 1 45 598 12 ILE N N 125.535 . 1 46 599 13 GLY H H 8.523 . 1 47 599 13 GLY C C 174.060 . 1 48 599 13 GLY CA C 45.400 . 1 49 599 13 GLY N N 113.331 . 1 50 600 14 ARG H H 8.209 . 1 51 600 14 ARG C C 176.124 . 1 52 600 14 ARG CA C 56.561 . 1 53 600 14 ARG CB C 31.025 . 1 54 600 14 ARG N N 120.632 . 1 55 601 15 ASP H H 8.502 . 1 56 601 15 ASP C C 176.583 . 1 57 601 15 ASP CA C 54.768 . 1 58 601 15 ASP CB C 40.956 . 1 59 601 15 ASP N N 120.677 . 1 60 602 16 ILE H H 7.967 . 1 61 602 16 ILE C C 176.643 . 1 62 602 16 ILE CA C 61.817 . 1 63 602 16 ILE CB C 38.502 . 1 64 602 16 ILE N N 120.813 . 1 65 603 17 LEU H H 8.181 . 1 66 603 17 LEU C C 178.044 . 1 67 603 17 LEU CA C 55.878 . 1 68 603 17 LEU CB C 42.095 . 1 69 603 17 LEU N N 124.369 . 1 70 604 18 GLU H H 8.269 . 1 71 604 18 GLU C C 176.983 . 1 72 604 18 GLU CA C 57.184 . 1 73 604 18 GLU CB C 30.048 . 1 74 604 18 GLU N N 120.835 . 1 75 605 19 GLN H H 8.247 . 1 76 605 19 GLN C C 176.424 . 1 77 605 19 GLN CA C 56.541 . 1 78 605 19 GLN CB C 29.290 . 1 79 605 19 GLN N N 120.414 . 1 80 606 20 GLN H H 8.347 . 1 81 606 20 GLN C C 176.379 . 1 82 606 20 GLN CA C 56.493 . 1 83 606 20 GLN CB C 29.333 . 1 84 606 20 GLN N N 120.453 . 1 85 607 21 SER H H 8.262 . 1 86 607 21 SER C C 174.644 . 1 87 607 21 SER CA C 58.689 . 1 88 607 21 SER CB C 63.669 . 1 89 607 21 SER N N 116.331 . 1 90 608 22 LEU H H 8.090 . 1 91 608 22 LEU C C 177.088 . 1 92 608 22 LEU CA C 55.613 . 1 93 608 22 LEU CB C 42.279 . 1 94 608 22 LEU N N 123.467 . 1 95 609 23 PHE H H 8.049 . 1 96 609 23 PHE C C 175.576 . 1 97 609 23 PHE CA C 57.598 . 1 98 609 23 PHE CB C 39.691 . 1 99 609 23 PHE N N 119.551 . 1 100 610 24 SER H H 7.973 . 1 101 610 24 SER C C 174.256 . 1 102 610 24 SER CA C 58.022 . 1 103 610 24 SER CB C 63.941 . 1 104 610 24 SER N N 116.731 . 1 105 611 25 PHE H H 8.231 . 1 106 611 25 PHE C C 175.637 . 1 107 611 25 PHE CA C 58.177 . 1 108 611 25 PHE CB C 39.399 . 1 109 611 25 PHE N N 122.206 . 1 110 612 26 ASP H H 8.243 . 1 111 612 26 ASP C C 175.948 . 1 112 612 26 ASP CA C 54.648 . 1 113 612 26 ASP CB C 41.109 . 1 114 612 26 ASP N N 120.645 . 1 115 613 27 ASN H H 8.209 . 1 116 613 27 ASN C C 175.375 . 1 117 613 27 ASN CA C 53.602 . 1 118 613 27 ASN CB C 38.862 . 1 119 613 27 ASN N N 118.595 . 1 120 614 28 VAL H H 7.978 . 1 121 614 28 VAL C C 176.391 . 1 122 614 28 VAL CA C 63.079 . 1 123 614 28 VAL CB C 32.481 . 1 124 614 28 VAL N N 119.809 . 1 125 615 29 LYS H H 8.257 . 1 126 615 29 LYS C C 176.242 . 1 127 615 29 LYS CA C 56.688 . 1 128 615 29 LYS CB C 32.794 . 1 129 615 29 LYS N N 123.482 . 1 130 616 30 ASN H H 8.232 . 1 131 616 30 ASN C C 174.854 . 1 132 616 30 ASN CA C 53.206 . 1 133 616 30 ASN CB C 38.992 . 1 134 616 30 ASN N N 118.842 . 1 135 617 31 PHE H H 8.119 . 1 136 617 31 PHE C C 175.771 . 1 137 617 31 PHE CA C 58.071 . 1 138 617 31 PHE CB C 39.530 . 1 139 617 31 PHE N N 120.648 . 1 140 618 32 ARG H H 8.237 . 1 141 618 32 ARG C C 175.834 . 1 142 618 32 ARG CA C 56.226 . 1 143 618 32 ARG CB C 30.861 . 1 144 618 32 ARG N N 122.437 . 1 145 619 33 ASP H H 8.255 . 1 146 619 33 ASP C C 176.869 . 1 147 619 33 ASP CA C 54.533 . 1 148 619 33 ASP CB C 41.194 . 1 149 619 33 ASP N N 121.421 . 1 150 620 34 GLY H H 8.374 . 1 151 620 34 GLY C C 174.551 . 1 152 620 34 GLY CA C 45.695 . 1 153 620 34 GLY N N 109.963 . 1 154 621 35 SER H H 8.255 . 1 155 621 35 SER C C 174.766 . 1 156 621 35 SER CA C 58.904 . 1 157 621 35 SER CB C 63.894 . 1 158 621 35 SER N N 115.835 . 1 159 622 36 LEU H H 8.264 . 1 160 622 36 LEU C C 177.590 . 1 161 622 36 LEU CA C 55.405 . 1 162 622 36 LEU CB C 42.282 . 1 163 622 36 LEU N N 123.483 . 1 164 623 37 THR H H 8.047 . 1 165 623 37 THR C C 174.134 . 1 166 623 37 THR CA C 61.772 . 1 167 623 37 THR CB C 69.736 . 1 168 623 37 THR N N 113.705 . 1 169 624 38 ASN H H 8.382 . 1 170 624 38 ASN C C 174.713 . 1 171 624 38 ASN CA C 53.231 . 1 172 624 38 ASN CB C 39.090 . 1 173 624 38 ASN N N 120.510 . 1 174 625 39 GLU H H 8.239 . 1 175 625 39 GLU C C 174.599 . 1 176 625 39 GLU CA C 54.499 . 1 177 625 39 GLU CB C 29.756 . 1 178 625 39 GLU N N 121.999 . 1 179 626 40 PRO C C 176.718 . 1 180 626 40 PRO CA C 63.425 . 1 181 626 40 PRO CB C 31.876 . 1 182 627 41 TYR H H 8.209 . 1 183 627 41 TYR C C 176.518 . 1 184 627 41 TYR CA C 58.065 . 1 185 627 41 TYR CB C 38.653 . 1 186 627 41 TYR N N 120.065 . 1 187 628 42 GLY H H 8.220 . 1 188 628 42 GLY C C 173.815 . 1 189 628 42 GLY CA C 45.376 . 1 190 628 42 GLY N N 110.868 . 1 191 629 43 ALA H H 8.032 . 1 192 629 43 ALA C C 177.549 . 1 193 629 43 ALA CA C 52.579 . 1 194 629 43 ALA CB C 19.323 . 1 195 629 43 ALA N N 123.669 . 1 196 630 44 ALA H H 8.220 . 1 197 630 44 ALA C C 177.888 . 1 198 630 44 ALA CA C 52.631 . 1 199 630 44 ALA CB C 19.026 . 1 200 630 44 ALA N N 122.782 . 1 201 631 45 VAL H H 7.981 . 1 202 631 45 VAL C C 176.064 . 1 203 631 45 VAL CA C 62.489 . 1 204 631 45 VAL CB C 32.790 . 1 205 631 45 VAL N N 119.108 . 1 206 632 46 GLN H H 8.353 . 1 207 632 46 GLN C C 175.679 . 1 208 632 46 GLN CA C 55.740 . 1 209 632 46 GLN CB C 29.539 . 1 210 632 46 GLN N N 123.881 . 1 211 633 47 LEU H H 8.317 . 1 212 633 47 LEU C C 177.215 . 1 213 633 47 LEU CA C 55.182 . 1 214 633 47 LEU CB C 42.419 . 1 215 633 47 LEU N N 124.182 . 1 216 634 48 ARG H H 8.286 . 1 217 634 48 ARG C C 176.535 . 1 218 634 48 ARG CA C 56.175 . 1 219 634 48 ARG CB C 30.835 . 1 220 634 48 ARG N N 122.055 . 1 221 635 49 GLU H H 8.582 . 1 222 635 49 GLU C C 176.179 . 1 223 635 49 GLU CA C 57.088 . 1 224 635 49 GLU CB C 30.259 . 1 225 635 49 GLU N N 121.986 . 1 226 636 50 ASP H H 8.421 . 1 227 636 50 ASP C C 175.829 . 1 228 636 50 ASP CA C 54.366 . 1 229 636 50 ASP CB C 40.787 . 1 230 636 50 ASP N N 119.889 . 1 231 637 51 LEU H H 7.920 . 1 232 637 51 LEU C C 176.758 . 1 233 637 51 LEU CA C 55.139 . 1 234 637 51 LEU CB C 42.563 . 1 235 637 51 LEU N N 121.511 . 1 236 638 52 ILE H H 8.040 . 1 237 638 52 ILE C C 175.837 . 1 238 638 52 ILE CA C 60.878 . 1 239 638 52 ILE CB C 38.355 . 1 240 638 52 ILE N N 122.392 . 1 241 639 53 LEU H H 8.286 . 1 242 639 53 LEU C C 175.165 . 1 243 639 53 LEU CA C 52.959 . 1 244 639 53 LEU CB C 41.735 . 1 245 639 53 LEU N N 128.097 . 1 246 640 54 PRO C C 176.937 . 1 247 640 54 PRO CA C 63.353 . 1 248 640 54 PRO CB C 32.093 . 1 249 641 55 GLU H H 8.441 . 1 250 641 55 GLU C C 176.340 . 1 251 641 55 GLU CA C 56.579 . 1 252 641 55 GLU CB C 30.057 . 1 253 641 55 GLU N N 120.300 . 1 254 642 56 LEU H H 8.158 . 1 255 642 56 LEU C C 176.756 . 1 256 642 56 LEU CA C 55.095 . 1 257 642 56 LEU CB C 42.426 . 1 258 642 56 LEU N N 122.999 . 1 259 643 57 ASN H H 8.331 . 1 260 643 57 ASN C C 174.984 . 1 261 643 57 ASN CA C 53.070 . 1 262 643 57 ASN CB C 39.037 . 1 263 643 57 ASN N N 119.358 . 1 264 644 58 PHE H H 8.235 . 1 265 644 58 PHE C C 175.814 . 1 266 644 58 PHE CA C 58.338 . 1 267 644 58 PHE CB C 39.517 . 1 268 644 58 PHE N N 121.245 . 1 269 645 59 GLU H H 8.329 . 1 270 645 59 GLU C C 176.563 . 1 271 645 59 GLU CA C 56.877 . 1 272 645 59 GLU CB C 30.227 . 1 273 645 59 GLU N N 121.813 . 1 274 646 60 GLU H H 8.359 . 1 275 646 60 GLU C C 177.029 . 1 276 646 60 GLU CA C 56.931 . 1 277 646 60 GLU CB C 30.229 . 1 278 646 60 GLU N N 121.652 . 1 279 647 61 THR H H 8.187 . 1 280 647 61 THR C C 174.790 . 1 281 647 61 THR CA C 62.529 . 1 282 647 61 THR CB C 69.760 . 1 283 647 61 THR N N 114.810 . 1 284 648 62 ASN H H 8.448 . 1 285 648 62 ASN C C 175.502 . 1 286 648 62 ASN CA C 53.821 . 1 287 648 62 ASN CB C 38.703 . 1 288 648 62 ASN N N 120.810 . 1 289 649 63 ALA H H 8.250 . 1 290 649 63 ALA C C 178.244 . 1 291 649 63 ALA CA C 53.412 . 1 292 649 63 ALA CB C 18.957 . 1 293 649 63 ALA N N 124.174 . 1 294 650 64 SER H H 8.187 . 1 295 650 64 SER C C 175.151 . 1 296 650 64 SER CA C 59.051 . 1 297 650 64 SER CB C 63.596 . 1 298 650 64 SER N N 114.184 . 1 stop_ save_