data_50098 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Nipah virus phosphoprotein, residues 1-100 ; _BMRB_accession_number 50098 _BMRB_flat_file_name bmr50098.str _Entry_type original _Submission_date 2019-11-29 _Accession_date 2019-11-29 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jensen Malene . . 2 Blackledge Martin . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 97 "13C chemical shifts" 285 "15N chemical shifts" 97 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-05-29 update BMRB 'update entry citation' 2020-01-10 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 50099 'Nipah virus phosphoprotein residues 91-190' 50100 'Nipah virus phosphoprotein residues 173-240' 50101 'Nipah virus phosphoprotein residues 223-319' 50102 'Nipah virus phosphoprotein residues 299-401' 50103 'Nipah virus phosphoprotein residues 387-479' 50105 'Nipah virus phosphoprotein residues 588-650' stop_ _Original_release_date 2019-12-02 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural Description of the Nipah Virus Phosphoprotein and Its Interaction With STAT1 ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 32348724 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jensen 'Malene Ringkjybing' R. . 2 Yabukarski Filip . . 3 Communie Guillaume . . 4 Condamine Eric . . 5 Mas Caroline . . 6 Volchkova Valentina . . 7 Tarbouriech Nicolas . . 8 Bourhis Jean-Marie M. . 9 Volchkov Viktor . . 10 Blackledge Martin . . 11 Jamin Marc . . stop_ _Journal_abbreviation 'Biophys. J.' _Journal_name_full 'Biophysical journal' _Journal_volume 118 _Journal_issue 10 _Journal_ISSN 1542-0086 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2470 _Page_last 2488 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly_1 _Saveframe_category molecular_system _Mol_system_name 'Nipah virus phosphoprotein, residues 1-100' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Nipah virus phosphoprotein' $entity_1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Nipah virus phosphoprotein, residues 1-100' _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 108 _Mol_residue_sequence ; MDKLELVNDGLNIIDFIQKN QKEIQKTYGRSSIQQPSIKD QTKAWEDFLQCTSGESEQVE GGMSKDDGDVERRNLEDLSS TSPTDGTIGKRVSNTRDWAE LEHHHHHH ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ASP 3 LYS 4 LEU 5 GLU 6 LEU 7 VAL 8 ASN 9 ASP 10 GLY 11 LEU 12 ASN 13 ILE 14 ILE 15 ASP 16 PHE 17 ILE 18 GLN 19 LYS 20 ASN 21 GLN 22 LYS 23 GLU 24 ILE 25 GLN 26 LYS 27 THR 28 TYR 29 GLY 30 ARG 31 SER 32 SER 33 ILE 34 GLN 35 GLN 36 PRO 37 SER 38 ILE 39 LYS 40 ASP 41 GLN 42 THR 43 LYS 44 ALA 45 TRP 46 GLU 47 ASP 48 PHE 49 LEU 50 GLN 51 CYS 52 THR 53 SER 54 GLY 55 GLU 56 SER 57 GLU 58 GLN 59 VAL 60 GLU 61 GLY 62 GLY 63 MET 64 SER 65 LYS 66 ASP 67 ASP 68 GLY 69 ASP 70 VAL 71 GLU 72 ARG 73 ARG 74 ASN 75 LEU 76 GLU 77 ASP 78 LEU 79 SER 80 SER 81 THR 82 SER 83 PRO 84 THR 85 ASP 86 GLY 87 THR 88 ILE 89 GLY 90 LYS 91 ARG 92 VAL 93 SER 94 ASN 95 THR 96 ARG 97 ASP 98 TRP 99 ALA 100 GLU 101 LEU 102 GLU 103 HIS 104 HIS 105 HIS 106 HIS 107 HIS 108 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source_1 _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 'Nipah virus' 121791 Virus . henipavirus 'Nipah virus' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . . . . plasmid na stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM '[U-13C; U-15N]' 'Bis-Tris ph 6.0' 20 mM 'natural abundance' NaCl 150 mM 'natural abundance' Arginine 50 mM 'natural abundance' glutamate 50 mM 'natural abundance' TCEP 0.5 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Agilent _Model Unity _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCACO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACO' _Sample_label $sample_1 save_ save_3D_HNCOCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCOCA' _Sample_label $sample_1 save_ save_3D_HNCA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCOCACB_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCOCACB' _Sample_label $sample_1 save_ save_3D_HNCACB_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 150 . mM pH 6.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 4.774 internal indirect . . . 0.251449530 water H 1 protons ppm 4.774 internal direct . . . 1 water N 15 protons ppm 4.774 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCO' '3D HNCACO' '3D HNCOCA' '3D HNCA' '3D HNCOCACB' '3D HNCACB' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name 'Nipah virus phosphoprotein' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 ASP H H 8.822 . 1 2 2 2 ASP C C 175.636 . 1 3 2 2 ASP CA C 54.424 . 1 4 2 2 ASP CB C 41.520 . 1 5 2 2 ASP N N 124.809 . 1 6 3 3 LYS H H 8.436 . 1 7 3 3 LYS C C 176.409 . 1 8 3 3 LYS CA C 56.756 . 1 9 3 3 LYS CB C 32.932 . 1 10 3 3 LYS N N 122.036 . 1 11 4 4 LEU H H 8.333 . 1 12 4 4 LEU C C 177.289 . 1 13 4 4 LEU CA C 55.442 . 1 14 4 4 LEU CB C 42.173 . 1 15 4 4 LEU N N 123.339 . 1 16 5 5 GLU H H 8.344 . 1 17 5 5 GLU C C 176.150 . 1 18 5 5 GLU CA C 56.435 . 1 19 5 5 GLU CB C 30.108 . 1 20 5 5 GLU N N 121.788 . 1 21 6 6 LEU H H 8.169 . 1 22 6 6 LEU C C 177.259 . 1 23 6 6 LEU CA C 55.172 . 1 24 6 6 LEU CB C 42.332 . 1 25 6 6 LEU N N 123.068 . 1 26 7 7 VAL H H 8.081 . 1 27 7 7 VAL C C 175.991 . 1 28 7 7 VAL CA C 62.462 . 1 29 7 7 VAL CB C 32.746 . 1 30 7 7 VAL N N 120.827 . 1 31 8 8 ASN H H 8.511 . 1 32 8 8 ASN C C 174.980 . 1 33 8 8 ASN CA C 53.296 . 1 34 8 8 ASN CB C 38.929 . 1 35 8 8 ASN N N 122.477 . 1 36 9 9 ASP H H 8.335 . 1 37 9 9 ASP C C 176.683 . 1 38 9 9 ASP CA C 54.612 . 1 39 9 9 ASP CB C 40.939 . 1 40 9 9 ASP N N 121.056 . 1 41 10 10 GLY H H 8.331 . 1 42 10 10 GLY C C 174.325 . 1 43 10 10 GLY CA C 45.735 . 1 44 10 10 GLY N N 108.573 . 1 45 11 11 LEU H H 8.010 . 1 46 11 11 LEU C C 177.089 . 1 47 11 11 LEU CA C 55.346 . 1 48 11 11 LEU CB C 42.448 . 1 49 11 11 LEU N N 121.139 . 1 50 12 12 ASN H H 8.513 . 1 51 12 12 ASN C C 175.192 . 1 52 12 12 ASN CA C 53.289 . 1 53 12 12 ASN CB C 38.805 . 1 54 12 12 ASN N N 119.624 . 1 55 13 13 ILE H H 8.092 . 1 56 13 13 ILE C C 176.360 . 1 57 13 13 ILE CA C 61.890 . 1 58 13 13 ILE CB C 38.539 . 1 59 13 13 ILE N N 121.420 . 1 60 14 14 ILE H H 8.066 . 1 61 14 14 ILE C C 176.279 . 1 62 14 14 ILE CA C 61.905 . 1 63 14 14 ILE CB C 38.463 . 1 64 14 14 ILE N N 123.593 . 1 65 15 15 ASP H H 8.110 . 1 66 15 15 ASP C C 176.297 . 1 67 15 15 ASP CA C 54.895 . 1 68 15 15 ASP CB C 41.111 . 1 69 15 15 ASP N N 122.785 . 1 70 16 16 PHE H H 8.062 . 1 71 16 16 PHE C C 176.199 . 1 72 16 16 PHE CA C 59.022 . 1 73 16 16 PHE CB C 39.377 . 1 74 16 16 PHE N N 120.356 . 1 75 17 17 ILE H H 8.042 . 1 76 17 17 ILE C C 176.747 . 1 77 17 17 ILE CA C 62.188 . 1 78 17 17 ILE N N 121.756 . 1 79 18 18 GLN H H 8.257 . 1 80 18 18 GLN C C 176.756 . 1 81 18 18 GLN CA C 56.621 . 1 82 18 18 GLN CB C 29.126 . 1 83 18 18 GLN N N 122.519 . 1 84 19 19 LYS H H 8.231 . 1 85 19 19 LYS C C 176.811 . 1 86 19 19 LYS CA C 57.273 . 1 87 19 19 LYS CB C 32.928 . 1 88 19 19 LYS N N 121.544 . 1 89 20 20 ASN H H 8.260 . 1 90 20 20 ASN C C 175.471 . 1 91 20 20 ASN CA C 53.674 . 1 92 20 20 ASN CB C 38.729 . 1 93 20 20 ASN N N 118.489 . 1 94 21 21 GLN H H 8.214 . 1 95 21 21 GLN C C 176.406 . 1 96 21 21 GLN CA C 56.867 . 1 97 21 21 GLN CB C 29.160 . 1 98 21 21 GLN N N 120.399 . 1 99 22 22 LYS H H 8.247 . 1 100 22 22 LYS C C 177.117 . 1 101 22 22 LYS CA C 57.263 . 1 102 22 22 LYS CB C 32.727 . 1 103 22 22 LYS N N 121.064 . 1 104 23 23 GLU H H 8.217 . 1 105 23 23 GLU C C 177.037 . 1 106 23 23 GLU CA C 56.957 . 1 107 23 23 GLU CB C 29.846 . 1 108 23 23 GLU N N 120.917 . 1 109 24 24 ILE H H 8.119 . 1 110 24 24 ILE C C 176.672 . 1 111 24 24 ILE CA C 61.919 . 1 112 24 24 ILE CB C 38.642 . 1 113 24 24 ILE N N 121.826 . 1 114 25 25 GLN H H 8.335 . 1 115 25 25 GLN C C 176.291 . 1 116 25 25 GLN CA C 56.344 . 1 117 25 25 GLN CB C 29.157 . 1 118 25 25 GLN N N 123.452 . 1 119 26 26 LYS H H 8.222 . 1 120 26 26 LYS C C 176.750 . 1 121 26 26 LYS CA C 56.880 . 1 122 26 26 LYS CB C 32.979 . 1 123 26 26 LYS N N 122.274 . 1 124 27 27 THR H H 8.003 . 1 125 27 27 THR C C 174.334 . 1 126 27 27 THR CA C 62.079 . 1 127 27 27 THR CB C 69.535 . 1 128 27 27 THR N N 114.233 . 1 129 28 28 TYR H H 8.203 . 1 130 28 28 TYR C C 176.352 . 1 131 28 28 TYR CA C 58.145 . 1 132 28 28 TYR CB C 38.879 . 1 133 28 28 TYR N N 121.957 . 1 134 29 29 GLY H H 8.306 . 1 135 29 29 GLY C C 174.124 . 1 136 29 29 GLY CA C 45.448 . 1 137 29 29 GLY N N 110.264 . 1 138 30 30 ARG H H 8.160 . 1 139 30 30 ARG C C 176.515 . 1 140 30 30 ARG CA C 56.223 . 1 141 30 30 ARG CB C 30.896 . 1 142 30 30 ARG N N 120.482 . 1 143 31 31 SER H H 8.413 . 1 144 31 31 SER C C 174.606 . 1 145 31 31 SER CA C 58.459 . 1 146 31 31 SER CB C 63.857 . 1 147 31 31 SER N N 116.786 . 1 148 32 32 SER H H 8.337 . 1 149 32 32 SER C C 174.416 . 1 150 32 32 SER CA C 58.485 . 1 151 32 32 SER CB C 63.835 . 1 152 32 32 SER N N 117.821 . 1 153 33 33 ILE H H 8.055 . 1 154 33 33 ILE C C 176.115 . 1 155 33 33 ILE CA C 61.279 . 1 156 33 33 ILE CB C 38.709 . 1 157 33 33 ILE N N 121.748 . 1 158 34 34 GLN H H 8.349 . 1 159 34 34 GLN C C 175.530 . 1 160 34 34 GLN CA C 55.717 . 1 161 34 34 GLN CB C 29.464 . 1 162 34 34 GLN N N 124.192 . 1 163 35 35 GLN H H 8.403 . 1 164 35 35 GLN C C 173.901 . 1 165 35 35 GLN CA C 53.736 . 1 166 35 35 GLN CB C 28.982 . 1 167 35 35 GLN N N 123.063 . 1 168 36 36 PRO C C 176.782 . 1 169 36 36 PRO CA C 63.223 . 1 170 36 36 PRO CB C 32.179 . 1 171 37 37 SER H H 8.491 . 1 172 37 37 SER C C 174.871 . 1 173 37 37 SER CA C 58.120 . 1 174 37 37 SER CB C 63.909 . 1 175 37 37 SER N N 116.312 . 1 176 38 38 ILE H H 8.223 . 1 177 38 38 ILE C C 176.619 . 1 178 38 38 ILE CA C 61.687 . 1 179 38 38 ILE CB C 38.449 . 1 180 38 38 ILE N N 122.872 . 1 181 39 39 LYS H H 8.279 . 1 182 39 39 LYS C C 176.569 . 1 183 39 39 LYS CA C 57.017 . 1 184 39 39 LYS CB C 32.798 . 1 185 39 39 LYS N N 124.392 . 1 186 40 40 ASP H H 8.157 . 1 187 40 40 ASP C C 176.719 . 1 188 40 40 ASP CA C 54.780 . 1 189 40 40 ASP CB C 41.013 . 1 190 40 40 ASP N N 120.918 . 1 191 41 41 GLN H H 8.400 . 1 192 41 41 GLN C C 176.688 . 1 193 41 41 GLN CA C 56.674 . 1 194 41 41 GLN CB C 29.192 . 1 195 41 41 GLN N N 121.314 . 1 196 42 42 THR H H 8.247 . 1 197 42 42 THR C C 175.040 . 1 198 42 42 THR CA C 63.383 . 1 199 42 42 THR CB C 69.335 . 1 200 42 42 THR N N 114.874 . 1 201 43 43 LYS H H 8.104 . 1 202 43 43 LYS C C 176.599 . 1 203 43 43 LYS CA C 56.694 . 1 204 43 43 LYS CB C 32.618 . 1 205 43 43 LYS N N 123.106 . 1 206 44 44 ALA H H 8.165 . 1 207 44 44 ALA C C 178.353 . 1 208 44 44 ALA CA C 53.330 . 1 209 44 44 ALA N N 123.919 . 1 210 45 45 TRP H H 7.993 . 1 211 45 45 TRP C C 176.738 . 1 212 45 45 TRP CA C 58.235 . 1 213 45 45 TRP CB C 29.506 . 1 214 45 45 TRP N N 119.740 . 1 215 46 46 GLU H H 8.121 . 1 216 46 46 GLU C C 176.932 . 1 217 46 46 GLU CA C 57.671 . 1 218 46 46 GLU CB C 29.753 . 1 219 46 46 GLU N N 120.677 . 1 220 47 47 ASP H H 8.148 . 1 221 47 47 ASP C C 176.801 . 1 222 47 47 ASP CA C 55.217 . 1 223 47 47 ASP CB C 40.726 . 1 224 47 47 ASP N N 120.133 . 1 225 48 48 PHE H H 7.972 . 1 226 48 48 PHE C C 176.398 . 1 227 48 48 PHE CA C 58.793 . 1 228 48 48 PHE CB C 39.138 . 1 229 48 48 PHE N N 120.468 . 1 230 49 49 LEU H H 8.030 . 1 231 49 49 LEU C C 177.677 . 1 232 49 49 LEU CA C 55.757 . 1 233 49 49 LEU CB C 42.121 . 1 234 49 49 LEU N N 121.798 . 1 235 50 50 GLN H H 8.082 . 1 236 50 50 GLN C C 176.269 . 1 237 50 50 GLN CA C 56.361 . 1 238 50 50 GLN CB C 29.143 . 1 239 50 50 GLN N N 119.604 . 1 240 51 51 CYS H H 8.220 . 1 241 51 51 CYS C C 175.115 . 1 242 51 51 CYS CA C 59.050 . 1 243 51 51 CYS CB C 27.819 . 1 244 51 51 CYS N N 119.533 . 1 245 52 52 THR H H 8.193 . 1 246 52 52 THR C C 174.744 . 1 247 52 52 THR CA C 61.980 . 1 248 52 52 THR CB C 69.785 . 1 249 52 52 THR N N 115.579 . 1 250 53 53 SER H H 8.278 . 1 251 53 53 SER C C 175.091 . 1 252 53 53 SER CA C 58.716 . 1 253 53 53 SER CB C 63.879 . 1 254 53 53 SER N N 117.922 . 1 255 54 54 GLY H H 8.412 . 1 256 54 54 GLY C C 174.283 . 1 257 54 54 GLY CA C 45.475 . 1 258 54 54 GLY N N 110.893 . 1 259 55 55 GLU H H 8.269 . 1 260 55 55 GLU C C 176.703 . 1 261 55 55 GLU CA C 56.623 . 1 262 55 55 GLU CB C 30.141 . 1 263 55 55 GLU N N 120.515 . 1 264 56 56 SER H H 8.362 . 1 265 56 56 SER C C 174.580 . 1 266 56 56 SER CA C 58.631 . 1 267 56 56 SER CB C 63.767 . 1 268 56 56 SER N N 116.561 . 1 269 57 57 GLU H H 8.423 . 1 270 57 57 GLU C C 176.313 . 1 271 57 57 GLU CA C 56.665 . 1 272 57 57 GLU CB C 30.030 . 1 273 57 57 GLU N N 122.520 . 1 274 58 58 GLN H H 8.366 . 1 275 58 58 GLN C C 175.936 . 1 276 58 58 GLN CA C 55.838 . 1 277 58 58 GLN CB C 29.498 . 1 278 58 58 GLN N N 121.424 . 1 279 59 59 VAL H H 8.179 . 1 280 59 59 VAL C C 176.298 . 1 281 59 59 VAL CA C 62.296 . 1 282 59 59 VAL CB C 32.903 . 1 283 59 59 VAL N N 121.556 . 1 284 60 60 GLU H H 8.560 . 1 285 60 60 GLU C C 177.003 . 1 286 60 60 GLU CA C 56.769 . 1 287 60 60 GLU CB C 29.964 . 1 288 60 60 GLU N N 124.920 . 1 289 61 61 GLY H H 8.508 . 1 290 61 61 GLY C C 174.778 . 1 291 61 61 GLY CA C 45.525 . 1 292 61 61 GLY N N 110.739 . 1 293 62 62 GLY H H 8.293 . 1 294 62 62 GLY C C 174.195 . 1 295 62 62 GLY CA C 45.378 . 1 296 62 62 GLY N N 108.636 . 1 297 63 63 MET H H 8.250 . 1 298 63 63 MET C C 176.321 . 1 299 63 63 MET CA C 55.506 . 1 300 63 63 MET CB C 33.100 . 1 301 63 63 MET N N 119.655 . 1 302 64 64 SER H H 8.429 . 1 303 64 64 SER C C 174.621 . 1 304 64 64 SER CA C 58.302 . 1 305 64 64 SER CB C 63.857 . 1 306 64 64 SER N N 117.446 . 1 307 65 65 LYS H H 8.447 . 1 308 65 65 LYS C C 176.312 . 1 309 65 65 LYS CA C 56.463 . 1 310 65 65 LYS CB C 33.011 . 1 311 65 65 LYS N N 123.545 . 1 312 66 66 ASP H H 8.354 . 1 313 66 66 ASP C C 175.997 . 1 314 66 66 ASP CA C 54.326 . 1 315 66 66 ASP CB C 41.229 . 1 316 66 66 ASP N N 121.387 . 1 317 67 67 ASP H H 8.275 . 1 318 67 67 ASP C C 176.939 . 1 319 67 67 ASP CA C 54.580 . 1 320 67 67 ASP CB C 41.012 . 1 321 67 67 ASP N N 121.242 . 1 322 68 68 GLY H H 8.417 . 1 323 68 68 GLY C C 174.247 . 1 324 68 68 GLY CA C 45.732 . 1 325 68 68 GLY N N 108.880 . 1 326 69 69 ASP H H 8.232 . 1 327 69 69 ASP C C 176.712 . 1 328 69 69 ASP CA C 54.673 . 1 329 69 69 ASP CB C 40.991 . 1 330 69 69 ASP N N 120.681 . 1 331 70 70 VAL H H 8.011 . 1 332 70 70 VAL C C 176.640 . 1 333 70 70 VAL CA C 63.347 . 1 334 70 70 VAL CB C 32.454 . 1 335 70 70 VAL N N 120.204 . 1 336 71 71 GLU H H 8.380 . 1 337 71 71 GLU C C 176.915 . 1 338 71 71 GLU CA C 57.218 . 1 339 71 71 GLU CB C 29.739 . 1 340 71 71 GLU N N 122.759 . 1 341 72 72 ARG H H 8.194 . 1 342 72 72 ARG C C 176.693 . 1 343 72 72 ARG CA C 56.655 . 1 344 72 72 ARG CB C 30.403 . 1 345 72 72 ARG N N 121.638 . 1 346 73 73 ARG H H 8.252 . 1 347 73 73 ARG C C 176.346 . 1 348 73 73 ARG CA C 56.708 . 1 349 73 73 ARG CB C 30.786 . 1 350 73 73 ARG N N 121.401 . 1 351 74 74 ASN H H 8.449 . 1 352 74 74 ASN C C 175.607 . 1 353 74 74 ASN CA C 53.567 . 1 354 74 74 ASN CB C 38.577 . 1 355 74 74 ASN N N 119.359 . 1 356 75 75 LEU H H 8.190 . 1 357 75 75 LEU C C 177.859 . 1 358 75 75 LEU CA C 55.899 . 1 359 75 75 LEU CB C 42.123 . 1 360 75 75 LEU N N 122.313 . 1 361 76 76 GLU H H 8.300 . 1 362 76 76 GLU C C 176.393 . 1 363 76 76 GLU CA C 57.056 . 1 364 76 76 GLU CB C 29.937 . 1 365 76 76 GLU N N 120.258 . 1 366 77 77 ASP H H 8.217 . 1 367 77 77 ASP C C 176.567 . 1 368 77 77 ASP CA C 54.412 . 1 369 77 77 ASP CB C 40.882 . 1 370 77 77 ASP N N 120.693 . 1 371 78 78 LEU H H 8.204 . 1 372 78 78 LEU C C 177.916 . 1 373 78 78 LEU CA C 55.516 . 1 374 78 78 LEU CB C 41.990 . 1 375 78 78 LEU N N 123.023 . 1 376 79 79 SER H H 8.309 . 1 377 79 79 SER C C 174.887 . 1 378 79 79 SER CA C 59.094 . 1 379 79 79 SER CB C 63.768 . 1 380 79 79 SER N N 115.913 . 1 381 80 80 SER H H 8.209 . 1 382 80 80 SER C C 174.784 . 1 383 80 80 SER CA C 58.569 . 1 384 80 80 SER CB C 63.802 . 1 385 80 80 SER N N 117.258 . 1 386 81 81 THR H H 8.109 . 1 387 81 81 THR C C 174.419 . 1 388 81 81 THR CA C 61.748 . 1 389 81 81 THR CB C 69.867 . 1 390 81 81 THR N N 115.113 . 1 391 82 82 SER H H 8.311 . 1 392 82 82 SER CA C 56.481 . 1 393 82 82 SER CB C 63.109 . 1 394 82 82 SER N N 119.531 . 1 395 83 83 PRO C C 177.424 . 1 396 83 83 PRO CA C 63.530 . 1 397 83 83 PRO CB C 32.167 . 1 398 84 84 THR H H 8.225 . 1 399 84 84 THR C C 174.668 . 1 400 84 84 THR CA C 61.846 . 1 401 84 84 THR CB C 69.699 . 1 402 84 84 THR N N 113.128 . 1 403 85 85 ASP H H 8.206 . 1 404 85 85 ASP C C 176.782 . 1 405 85 85 ASP CA C 54.466 . 1 406 85 85 ASP CB C 41.143 . 1 407 85 85 ASP N N 122.332 . 1 408 86 86 GLY H H 8.396 . 1 409 86 86 GLY C C 174.690 . 1 410 86 86 GLY CA C 45.658 . 1 411 86 86 GLY N N 109.473 . 1 412 87 87 THR H H 8.150 . 1 413 87 87 THR C C 174.956 . 1 414 87 87 THR CA C 62.462 . 1 415 87 87 THR CB C 69.879 . 1 416 87 87 THR N N 113.794 . 1 417 88 88 ILE H H 8.096 . 1 418 88 88 ILE C C 176.794 . 1 419 88 88 ILE CA C 61.658 . 1 420 88 88 ILE CB C 38.537 . 1 421 88 88 ILE N N 122.818 . 1 422 89 89 GLY H H 8.431 . 1 423 89 89 GLY C C 173.946 . 1 424 89 89 GLY CA C 45.320 . 1 425 89 89 GLY N N 112.594 . 1 426 90 90 LYS H H 8.081 . 1 427 90 90 LYS C C 176.497 . 1 428 90 90 LYS CA C 56.237 . 1 429 90 90 LYS CB C 33.073 . 1 430 90 90 LYS N N 120.883 . 1 431 91 91 ARG H H 8.363 . 1 432 91 91 ARG C C 176.341 . 1 433 91 91 ARG CA C 56.176 . 1 434 91 91 ARG CB C 30.759 . 1 435 91 91 ARG N N 122.789 . 1 436 92 92 VAL H H 8.237 . 1 437 92 92 VAL C C 176.134 . 1 438 92 92 VAL CA C 62.286 . 1 439 92 92 VAL CB C 32.836 . 1 440 92 92 VAL N N 121.661 . 1 441 93 93 SER H H 8.377 . 1 442 93 93 SER C C 174.251 . 1 443 93 93 SER CA C 58.250 . 1 444 93 93 SER CB C 63.906 . 1 445 93 93 SER N N 119.141 . 1 446 94 94 ASN H H 8.506 . 1 447 94 94 ASN C C 175.440 . 1 448 94 94 ASN CA C 53.306 . 1 449 94 94 ASN CB C 38.936 . 1 450 94 94 ASN N N 121.247 . 1 451 95 95 THR H H 8.124 . 1 452 95 95 THR C C 174.753 . 1 453 95 95 THR CA C 62.331 . 1 454 95 95 THR CB C 69.471 . 1 455 95 95 THR N N 114.180 . 1 456 96 96 ARG H H 8.277 . 1 457 96 96 ARG C C 176.068 . 1 458 96 96 ARG CA C 56.524 . 1 459 96 96 ARG CB C 30.492 . 1 460 96 96 ARG N N 122.803 . 1 461 97 97 ASP H H 8.258 . 1 462 97 97 ASP C C 176.498 . 1 463 97 97 ASP CA C 54.446 . 1 464 97 97 ASP CB C 40.745 . 1 465 97 97 ASP N N 121.005 . 1 466 98 98 TRP H H 7.999 . 1 467 98 98 TRP C C 176.742 . 1 468 98 98 TRP CA C 58.367 . 1 469 98 98 TRP CB C 29.256 . 1 470 98 98 TRP N N 121.926 . 1 471 99 99 ALA H H 8.062 . 1 472 99 99 ALA C C 178.407 . 1 473 99 99 ALA CA C 53.584 . 1 474 99 99 ALA N N 123.625 . 1 475 100 100 GLU H H 7.971 . 1 476 100 100 GLU C C 177.261 . 1 477 100 100 GLU CA C 57.283 . 1 478 100 100 GLU CB C 29.697 . 1 479 100 100 GLU N N 118.584 . 1 stop_ save_