data_30794 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of the HIV-1 PBS-segment ; _BMRB_accession_number 30794 _BMRB_flat_file_name bmr30794.str _Entry_type original _Submission_date 2020-09-08 _Accession_date 2020-09-08 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Heng X. . . 2 Song Z. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 273 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-09-16 original BMRB . stop_ _Original_release_date 2020-09-14 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structure of the HIV-1 PBS-segment is conserved for viral infectivity ; _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Song Z. . . 2 Heng X. . . stop_ _Journal_abbreviation . _Journal_volume . _Journal_issue . _Journal_CSD 0353 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'RNA (103-MER)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label unit_1 $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common entity_1 _Molecular_mass 33249.836 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 103 _Mol_residue_sequence ; GGCUCUGGUAACUAGAGAUC CCUCAGACCCUUUUAGUCAG UGUGGAAAAUCUCUAGCAGU GGCGCCCGAACAGGGACUUG AAAGCGAAAGUAAAGCCAGA GCC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 123 G 2 124 G 3 125 C 4 126 U 5 127 C 6 128 U 7 129 G 8 130 G 9 131 U 10 132 A 11 133 A 12 134 C 13 135 U 14 136 A 15 137 G 16 138 A 17 139 G 18 140 A 19 141 U 20 142 C 21 143 C 22 144 C 23 145 U 24 146 C 25 147 A 26 148 G 27 149 A 28 150 C 29 151 C 30 152 C 31 153 U 32 154 U 33 155 U 34 156 U 35 157 A 36 158 G 37 159 U 38 160 C 39 161 A 40 162 G 41 163 U 42 164 G 43 165 U 44 166 G 45 167 G 46 168 A 47 169 A 48 170 A 49 171 A 50 172 U 51 173 C 52 174 U 53 175 C 54 176 U 55 177 A 56 178 G 57 179 C 58 180 A 59 181 G 60 182 U 61 183 G 62 184 G 63 185 C 64 186 G 65 187 C 66 188 C 67 189 C 68 190 G 69 191 A 70 192 A 71 193 C 72 194 A 73 195 G 74 196 G 75 197 G 76 198 A 77 199 C 78 200 U 79 201 U 80 202 G 81 203 A 82 204 A 83 205 A 84 206 G 85 207 C 86 208 G 87 209 A 88 210 A 89 211 A 90 212 G 91 213 U 92 214 A 93 215 A 94 216 A 95 217 G 96 218 C 97 219 C 98 220 A 99 221 G 100 222 A 101 223 G 102 224 C 103 225 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 HIV-1 11676 Viruses . Lentivirus HIV-1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '250 uM RNA (103-MER), 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 250 uM 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '250 uM A-8D, C-D, G-H, U-5,6-D2 RNA (103-MER), 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 250 uM '[A-8D, C-D, G-H, U-5,6-D2]' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details '250 uM A-8D, C-D, G-8D, U-H RNA (103-MER), 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 250 uM '[A-8D, C-D, G-8D, U-H]' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details '250 uM A-8D, C-5,6-D2, G-D, U-5,6-D2 RNA (103-MER), 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 250 uM '[A-8D, C-5,6-D2, G-D, U-5,6-D2]' stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details '250 uM A-8D, C-D, G-8D, U-D RNA (103-MER), 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 250 uM '[A-8D, C-D, G-8D, U-D]' stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details '250 uM A-H, C-D, G-H, U-D RNA (103-MER), 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 250 uM '[A-H, C-D, G-H, U-D]' stop_ save_ save_sample_7 _Saveframe_category sample _Sample_type solution _Details '250 uM A-H, C-H, G-D, U-D RNA (103-MER), 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 250 uM '[A-H, C-H, G-D, U-D]' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_2 _Saveframe_category software _Name NMRDraw _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_3 _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ save_software_4 _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'geometry optimization' 'structure calculation' stop_ _Details . save_ save_software_5 _Saveframe_category software _Name Amber _Version . loop_ _Vendor _Address _Electronic_address 'Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_software_6 _Saveframe_category software _Name 'X-PLOR NIH' _Version . loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AVANCE _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_3 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_4 save_ save_2D_1H-1H_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_5 save_ save_2D_1H-1H_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_6 save_ save_2D_1H-1H_NOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_7 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 3 . mM pH 7.5 . pD pressure 1 . atm temperature 308 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.701 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 $sample_2 $sample_3 $sample_4 $sample_5 $sample_6 $sample_7 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name unit_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 123 1 G H1' H 5.8712 0.0000 1 2 123 1 G H8 H 8.1317 0.0000 1 3 124 2 G H1' H 5.8795 0.0000 1 4 124 2 G H8 H 7.5070 0.0000 1 5 125 3 C H1' H 5.5505 0.0000 1 6 125 3 C H5 H 5.2840 0.0000 1 7 125 3 C H6 H 7.5920 0.0000 1 8 126 4 U H1' H 5.5752 0.0000 1 9 126 4 U H5 H 5.4607 0.0000 1 10 126 4 U H6 H 7.8438 0.0000 1 11 127 5 C H1' H 5.5015 0.0000 1 12 127 5 C H5 H 5.6490 0.0000 1 13 127 5 C H6 H 7.8173 0.0000 1 14 128 6 U H1' H 5.4656 0.0000 1 15 128 6 U H5 H 5.3610 0.0000 1 16 128 6 U H6 H 7.7938 0.0000 1 17 129 7 G H1' H 5.7582 0.0000 1 18 129 7 G H8 H 7.7014 0.0000 1 19 130 8 G H1' H 5.6678 0.0000 1 20 130 8 G H8 H 7.1606 0.0000 1 21 131 9 U H1' H 5.3508 0.0000 1 22 131 9 U H5 H 5.3344 0.0000 1 23 131 9 U H6 H 7.5108 0.0000 1 24 132 10 A H1' H 5.5357 0.0000 1 25 132 10 A H2 H 7.9043 0.0000 1 26 132 10 A H8 H 8.0035 0.0000 1 27 133 11 A H1' H 5.8797 0.0000 1 28 133 11 A H2 H 7.5691 0.0000 1 29 133 11 A H8 H 8.1804 0.0000 1 30 134 12 C H1' H 5.9069 0.0000 1 31 134 12 C H5 H 5.5600 0.0000 1 32 134 12 C H6 H 7.6126 0.0000 1 33 135 13 U H1' H 5.7257 0.0000 1 34 136 14 A H1' H 5.9604 0.0000 1 35 136 14 A H2 H 6.8311 0.0000 1 36 136 14 A H8 H 8.1437 0.0000 1 37 137 15 G H1' H 5.5211 0.0000 1 38 137 15 G H8 H 7.1400 0.0000 1 39 138 16 A H1' H 5.8709 0.0000 1 40 138 16 A H2 H 7.3990 0.0000 1 41 138 16 A H8 H 7.5893 0.0000 1 42 139 17 G H1' H 5.5679 0.0000 1 43 139 17 G H8 H 7.0660 0.0000 1 44 140 18 A H1' H 6.0069 0.0000 1 45 140 18 A H2 H 7.8637 0.0000 1 46 140 18 A H8 H 7.7873 0.0000 1 47 141 19 U H1' H 5.6334 0.0000 1 48 141 19 U H5 H 5.3743 0.0000 1 49 141 19 U H6 H 7.6379 0.0000 1 50 142 20 C H1' H 5.5964 0.0000 1 51 142 20 C H5 H 5.8245 0.0000 1 52 142 20 C H6 H 7.8854 0.0000 1 53 143 21 C H1' H 5.4811 0.0000 1 54 143 21 C H5 H 5.5894 0.0000 1 55 143 21 C H6 H 7.7415 0.0000 1 56 144 22 C H1' H 5.4276 0.0000 1 57 144 22 C H5 H 5.4074 0.0000 1 58 144 22 C H6 H 7.6465 0.0000 1 59 145 23 U H5 H 5.7744 0.0000 1 60 145 23 U H6 H 7.8067 0.0000 1 61 146 24 C H1' H 5.5262 0.0000 1 62 146 24 C H5 H 5.7198 0.0000 1 63 146 24 C H6 H 7.8285 0.0000 1 64 147 25 A H1' H 6.0637 0.0000 1 65 147 25 A H2 H 7.8261 0.0000 1 66 147 25 A H8 H 8.1457 0.0000 1 67 148 26 G H1' H 5.6191 0.0000 1 68 148 26 G H8 H 7.4585 0.0000 1 69 149 27 A H1' H 5.9484 0.0000 1 70 149 27 A H2 H 7.7124 0.0000 1 71 149 27 A H8 H 7.8833 0.0000 1 72 150 28 C H1' H 5.3583 0.0000 1 73 150 28 C H5 H 5.0382 0.0000 1 74 150 28 C H6 H 7.2146 0.0000 1 75 151 29 C H1' H 5.3639 0.0000 1 76 151 29 C H5 H 5.6152 0.0000 1 77 151 29 C H6 H 7.7175 0.0000 1 78 152 30 C H1' H 5.6290 0.0000 1 79 152 30 C H5 H 5.6917 0.0000 1 80 152 30 C H6 H 7.7132 0.0000 1 81 153 31 U H1' H 5.9013 0.0000 1 82 153 31 U H5 H 5.8312 0.0000 1 83 153 31 U H6 H 7.7687 0.0000 1 84 154 32 U H1' H 5.8906 0.0000 1 85 154 32 U H5 H 5.8444 0.0000 1 86 154 32 U H6 H 7.7493 0.0000 1 87 155 33 U H1' H 5.4562 0.0000 1 88 155 33 U H5 H 5.5112 0.0000 1 89 155 33 U H6 H 7.7547 0.0000 1 90 156 34 U H1' H 5.9310 0.0000 1 91 156 34 U H5 H 5.8677 0.0000 1 92 156 34 U H6 H 7.7953 0.0000 1 93 157 35 A H1' H 6.1070 0.0000 1 94 157 35 A H2 H 8.0503 0.0000 1 95 157 35 A H8 H 8.3832 0.0000 1 96 158 36 G H1' H 5.4611 0.0000 1 97 158 36 G H8 H 7.3668 0.0000 1 98 159 37 U H1' H 5.5965 0.0000 1 99 159 37 U H5 H 5.1113 0.0000 1 100 159 37 U H6 H 7.7501 0.0000 1 101 160 38 C H1' H 5.5059 0.0000 1 102 160 38 C H5 H 5.5998 0.0000 1 103 160 38 C H6 H 7.7126 0.0000 1 104 161 39 A H1' H 5.9024 0.0000 1 105 161 39 A H2 H 7.5171 0.0000 1 106 161 39 A H8 H 8.0684 0.0000 1 107 162 40 G H1' H 5.3536 0.0000 1 108 162 40 G H8 H 7.1902 0.0000 1 109 163 41 U H1' H 5.4032 0.0000 1 110 163 41 U H5 H 5.2078 0.0000 1 111 163 41 U H6 H 7.4599 0.0000 1 112 164 42 G H1' H 5.8623 0.0000 1 113 164 42 G H8 H 7.8944 0.0000 1 114 165 43 U H1' H 5.8134 0.0000 1 115 165 43 U H5 H 5.6656 0.0000 1 116 165 43 U H6 H 7.6651 0.0000 1 117 166 44 G H1' H 5.7072 0.0000 1 118 166 44 G H8 H 7.4645 0.0000 1 119 167 45 G H1' H 5.7773 0.0000 1 120 167 45 G H8 H 7.3004 0.0000 1 121 168 46 A H1' H 5.8388 0.0000 1 122 168 46 A H2 H 7.4861 0.0000 1 123 168 46 A H8 H 7.7095 0.0000 1 124 169 47 A H1' H 5.5410 0.0000 1 125 169 47 A H2 H 7.5520 0.0000 1 126 169 47 A H8 H 7.7926 0.0000 1 127 170 48 A H1' H 5.7695 0.0000 1 128 170 48 A H2 H 7.8117 0.0000 1 129 170 48 A H8 H 8.0303 0.0000 1 130 171 49 A H1' H 5.8015 0.0000 1 131 171 49 A H2 H 7.9097 0.0000 1 132 171 49 A H8 H 8.1591 0.0000 1 133 172 50 U H1' H 5.6397 0.0000 1 134 172 50 U H5 H 5.3498 0.0000 1 135 172 50 U H6 H 7.7579 0.0000 1 136 173 51 C H1' H 5.6006 0.0000 1 137 173 51 C H5 H 5.7239 0.0000 1 138 173 51 C H6 H 7.8846 0.0000 1 139 174 52 U H1' H 5.5042 0.0000 1 140 174 52 U H5 H 5.4157 0.0000 1 141 174 52 U H6 H 7.8958 0.0000 1 142 175 53 C H1' H 5.5003 0.0000 1 143 175 53 C H5 H 5.6706 0.0000 1 144 175 53 C H6 H 7.8162 0.0000 1 145 176 54 U H1' H 5.4000 0.0000 1 146 176 54 U H5 H 5.4008 0.0000 1 147 176 54 U H6 H 7.7900 0.0000 1 148 177 55 A H1' H 5.9040 0.0000 1 149 177 55 A H2 H 7.5288 0.0000 1 150 177 55 A H8 H 8.0624 0.0000 1 151 178 56 G H1' H 5.8762 0.0000 1 152 178 56 G H8 H 8.1890 0.0000 1 153 179 57 C H1' H 5.6000 0.0000 1 154 179 57 C H5 H 5.3703 0.0000 1 155 179 57 C H6 H 7.5315 0.0000 1 156 180 58 A H1' H 5.8729 0.0000 1 157 180 58 A H8 H 8.1225 0.0000 1 158 181 59 G H1' H 5.6233 0.0000 1 159 181 59 G H8 H 7.7323 0.0000 1 160 182 60 U H1' H 5.6607 0.0000 1 161 182 60 U H5 H 5.6881 0.0000 1 162 182 60 U H6 H 7.6203 0.0000 1 163 183 61 G H1' H 5.7458 0.0000 1 164 183 61 G H8 H 7.8843 0.0000 1 165 184 62 G H1' H 5.3996 0.0000 1 166 184 62 G H8 H 7.8476 0.0000 1 167 185 63 C H1' H 5.8369 0.0000 1 168 185 63 C H5 H 5.8988 0.0000 1 169 185 63 C H6 H 7.8125 0.0000 1 170 187 65 C H1' H 5.5395 0.0000 1 171 187 65 C H5 H 5.4119 0.0000 1 172 187 65 C H6 H 7.6774 0.0000 1 173 188 66 C H1' H 5.4421 0.0000 1 174 188 66 C H5 H 5.4752 0.0000 1 175 188 66 C H6 H 7.8201 0.0000 1 176 189 67 C H1' H 5.4640 0.0000 1 177 189 67 C H5 H 5.3226 0.0000 1 178 189 67 C H6 H 7.5194 0.0000 1 179 190 68 G H1' H 5.5780 0.0000 1 180 190 68 G H8 H 7.6193 0.0000 1 181 191 69 A H1' H 5.7109 0.0000 1 182 191 69 A H8 H 7.9999 0.0000 1 183 192 70 A H1' H 5.8804 0.0000 1 184 192 70 A H2 H 8.0340 0.0000 1 185 192 70 A H8 H 8.1509 0.0000 1 186 193 71 C H1' H 5.7846 0.0000 1 187 193 71 C H5 H 5.7879 0.0000 1 188 193 71 C H6 H 7.6388 0.0000 1 189 194 72 A H1' H 6.0386 0.0000 1 190 194 72 A H2 H 8.1264 0.0000 1 191 194 72 A H8 H 8.1696 0.0000 1 192 195 73 G H1' H 4.9483 0.0000 1 193 195 73 G H8 H 7.7341 0.0000 1 194 196 74 G H1' H 5.7628 0.0000 1 195 196 74 G H8 H 7.1880 0.0000 1 196 197 75 G H1' H 5.7174 0.0000 1 197 197 75 G H8 H 7.1725 0.0000 1 198 198 76 A H1' H 5.9984 0.0000 1 199 198 76 A H2 H 8.0221 0.0000 1 200 198 76 A H8 H 7.8386 0.0000 1 201 199 77 C H1' H 5.4996 0.0000 1 202 199 77 C H5 H 5.3981 0.0000 1 203 199 77 C H6 H 7.4253 0.0000 1 204 200 78 U H1' H 5.7467 0.0000 1 205 200 78 U H5 H 5.6023 0.0000 1 206 200 78 U H6 H 7.6831 0.0000 1 207 201 79 U H1' H 5.6310 0.0000 1 208 201 79 U H5 H 5.5459 0.0000 1 209 201 79 U H6 H 7.8559 0.0000 1 210 202 80 G H1' H 5.5550 0.0000 1 211 202 80 G H8 H 7.7637 0.0000 1 212 203 81 A H1' H 5.8481 0.0000 1 213 203 81 A H2 H 7.7943 0.0000 1 214 203 81 A H8 H 8.1459 0.0000 1 215 204 82 A H1' H 5.6997 0.0000 1 216 204 82 A H2 H 7.7758 0.0000 1 217 204 82 A H8 H 8.0704 0.0000 1 218 205 83 A H1' H 5.8947 0.0000 1 219 205 83 A H2 H 8.0034 0.0000 1 220 205 83 A H8 H 8.0969 0.0000 1 221 206 84 G H1' H 5.2967 0.0000 1 222 206 84 G H8 H 7.6954 0.0000 1 223 207 85 C H1' H 5.5614 0.0000 1 224 207 85 C H5 H 5.3154 0.0000 1 225 207 85 C H6 H 7.4818 0.0000 1 226 208 86 G H1' H 5.6279 0.0000 1 227 208 86 G H8 H 7.5806 0.0000 1 228 209 87 A H1' H 5.5943 0.0000 1 229 209 87 A H2 H 7.7042 0.0000 1 230 209 87 A H8 H 7.9291 0.0000 1 231 210 88 A H1' H 5.6460 0.0000 1 232 210 88 A H2 H 7.7602 0.0000 1 233 210 88 A H8 H 7.9230 0.0000 1 234 211 89 A H1' H 5.7211 0.0000 1 235 211 89 A H2 H 7.8139 0.0000 1 236 211 89 A H8 H 7.9931 0.0000 1 237 212 90 G H1' H 5.5495 0.0000 1 238 212 90 G H8 H 7.6676 0.0000 1 239 213 91 U H1' H 5.6665 0.0000 1 240 213 91 U H5 H 5.4888 0.0000 1 241 213 91 U H6 H 7.5675 0.0000 1 242 214 92 A H1' H 5.7816 0.0000 1 243 214 92 A H2 H 7.5318 0.0000 1 244 214 92 A H8 H 8.1046 0.0000 1 245 215 93 A H1' H 5.6243 0.0000 1 246 215 93 A H2 H 7.6703 0.0000 1 247 215 93 A H8 H 7.9981 0.0000 1 248 216 94 A H1' H 5.9541 0.0000 1 249 216 94 A H2 H 8.0317 0.0000 1 250 216 94 A H8 H 8.2119 0.0000 1 251 217 95 G H1' H 5.5348 0.0000 1 252 217 95 G H8 H 7.8669 0.0000 1 253 218 96 C H1' H 5.4952 0.0000 1 254 218 96 C H5 H 5.3921 0.0000 1 255 218 96 C H6 H 7.6438 0.0000 1 256 219 97 C H1' H 5.4855 0.0000 1 257 219 97 C H5 H 5.4756 0.0000 1 258 219 97 C H6 H 7.7257 0.0000 1 259 220 98 A H1' H 5.9066 0.0000 1 260 220 98 A H2 H 6.9088 0.0000 1 261 220 98 A H8 H 7.9450 0.0000 1 262 221 99 G H1' H 5.5433 0.0000 1 263 221 99 G H8 H 7.0892 0.0000 1 264 222 100 A H1' H 5.9053 0.0000 1 265 222 100 A H2 H 7.4036 0.0000 1 266 222 100 A H8 H 7.6340 0.0000 1 267 223 101 G H1' H 5.6466 0.0000 1 268 223 101 G H8 H 7.1869 0.0000 1 269 224 102 C H1' H 5.5211 0.0000 1 270 224 102 C H5 H 5.1443 0.0000 1 271 224 102 C H6 H 7.5937 0.0000 1 272 225 103 C H5 H 5.4233 0.0000 1 273 225 103 C H6 H 7.6163 0.0000 1 stop_ save_