data_30512 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of 7SK stem-loop 1 ; _BMRB_accession_number 30512 _BMRB_flat_file_name bmr30512.str _Entry_type original _Submission_date 2018-08-31 _Accession_date 2018-08-31 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Pham V. V. . 2 D'Souza V. M. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 207 "13C chemical shifts" 49 "15N chemical shifts" 27 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-10-24 original BMRB . stop_ _Original_release_date 2018-09-10 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 30323330 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Pham V. V. . 2 Salguero C. . . 3 Khan S. N. . 4 Meagher J. . . 5 Brown W. . . 6 Humbert N. . . 7 'de Rocquigny' H. . . 8 Smith J. . . 9 D'Souza V. M. . stop_ _Journal_abbreviation 'Nat. Commun.' _Journal_name_full 'Nature communications' _Journal_volume 9 _Journal_issue 1 _Journal_ISSN 2041-1723 _Journal_CSD 0353 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4266 _Page_last 4266 _Year 2018 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name '7SK RNA' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common entity_1 _Molecular_mass 18358.910 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 57 _Mol_residue_sequence ; GGGAUCUGUCACCCCAUUGA UCGCCGAGAGGCUGAUCUGG XUGGXUAGGCGGGUCCC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 24 G 2 25 G 3 26 G 4 27 A 5 28 U 6 29 C 7 30 U 8 31 G 9 32 U 10 33 C 11 34 A 12 35 C 13 36 C 14 37 C 15 38 C 16 39 A 17 40 U 18 41 U 19 42 G 20 43 A 21 44 U 22 45 C 23 46 G 24 47 C 25 48 C 26 49 G 27 50 A 28 51 G 29 52 A 30 60 G 31 61 G 32 62 C 33 63 U 34 64 G 35 65 A 36 66 U 37 67 C 38 68 U 39 69 G 40 70 G 41 71 RY 42 72 U 43 73 G 44 74 G 45 75 RY 46 76 U 47 77 A 48 78 G 49 79 G 50 80 C 51 81 G 52 82 G 53 83 G 54 84 U 55 85 C 56 86 C 57 87 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ ###################### # Polymer residues # ###################### save_chem_comp_RY _Saveframe_category polymer_residue _Mol_type 'RNA LINKING' _Name_common "5'-3,6-dihydrocytidylic acid" _BMRB_code RY _PDB_code RY _Standard_residue_derivative . _Molecular_mass 325.212 _Mol_paramagnetic . _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons P P P . 0 . ? C5' C5' C . 0 . ? O5' O5' O . 0 . ? C4' C4' C . 0 . ? O4' O4' O . 0 . ? C3' C3' C . 0 . ? O3' O3' O . 0 . ? C2' C2' C . 0 . ? O2' O2' O . 0 . ? C1' C1' C . 0 . ? N1 N1 N . 0 . ? C2 C2 C . 0 . ? O2 O2 O . 0 . ? N3 N3 N . 0 . ? C4 C4 C . 0 . ? N4 N4 N . 0 . ? C5 C5 C . 0 . ? C6 C6 C . 0 . ? OP1 OP1 O . 0 . ? OP2 OP2 O . 0 . ? OP3 OP3 O . 0 . ? H5'' H5'' H . 0 . ? H5' H5' H . 0 . ? H4' H4' H . 0 . ? H3' H3' H . 0 . ? H1 H1 H . 0 . ? H2' H2' H . 0 . ? HO2' HO2' H . 0 . ? H1' H1' H . 0 . ? H3 H3 H . 0 . ? H41 H41 H . 0 . ? H42 H42 H . 0 . ? H5 H5 H . 0 . ? H6 H6 H . 0 . ? H2 H2 H . 0 . ? H4 H4 H . 0 . ? H7 H7 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name DOUB OP1 P ? ? DOUB O2 C2 ? ? SING O3' C3' ? ? SING O2' C2' ? ? SING P OP2 ? ? SING P O5' ? ? SING C2 N3 ? ? SING C2 N1 ? ? SING C3' C4' ? ? SING C3' C2' ? ? SING C4' O4' ? ? SING C4' C5' ? ? SING C1' C2' ? ? SING C1' O4' ? ? SING C1' N1 ? ? SING N3 C4 ? ? SING N1 C6 ? ? SING C5' O5' ? ? SING C4 N4 ? ? DOUB C4 C5 ? ? SING C6 C5 ? ? SING P OP3 ? ? SING C5' H5'' ? ? SING C5' H5' ? ? SING C4' H4' ? ? SING C3' H3' ? ? SING O3' H1 ? ? SING C2' H2' ? ? SING O2' HO2' ? ? SING C1' H1' ? ? SING N3 H3 ? ? SING N4 H41 ? ? SING N4 H42 ? ? SING C5 H5 ? ? SING C6 H6 ? ? SING C6 H2 ? ? SING OP2 H4 ? ? SING OP3 H7 ? ? stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '0.5 mM 7SK RNA, 10mM Phosphate, 70mM NaCl, 0.1mM EDTA, 90%H2O/10%D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM 'natural abundance' NaCl 10 mM 'natural abundance' NaCl 70 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '0.5 mM [U-13C; U-15N] 7SK RNA, 10mM Phosphate, 70mM NaCl, 0.1mM EDTA, 90%H2O/10%D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM '[U-13C; U-15N]' NaCl 10 mM 'natural abundance' NaCl 70 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details '0.5 mM [U-13C; U-15N] 7SK RNA, 10mM Phosphate, 70mM NaCl, 0.1mM EDTA, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM '[U-13C; U-15N]' NaCl 10 mM 'natural abundance' NaCl 70 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details '0.5 mM 7SK RNA, 10mM Phosphate, 70mM NaCl, 0.1mM EDTA, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM 'natural abundance' NaCl 10 mM 'natural abundance' NaCl 70 mM 'natural abundance' EDTA 0.1 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_software_2 _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ save_software_3 _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'data analysis' processing 'structure calculation' stop_ _Details . save_ save_software_4 _Saveframe_category software _Name AMBER _Version . loop_ _Vendor _Address _Electronic_address 'Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_3 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_4 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 80 . mM pH 5.4 . pH pressure 100000 . Pa temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl carbons' Hz 25144953 internal indirect . . . 0.25144953 water H 1 protons ppm 4.706 na direct . . . 1 'liquid anhydrous ammonia' N 15 nitrogen Hz 10132911.8 external indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $software_3 stop_ loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-15N HSQC' '2D 1H-13C HSQC' stop_ loop_ _Sample_label $sample_1 $sample_2 $sample_3 $sample_4 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 24 1 G H1 H 11.7423 . 1 2 24 1 G H1' H 5.5422 . 1 3 24 1 G H2' H 4.6704 . 1 4 24 1 G H8 H 7.8907 . 1 5 24 1 G C1' C 90.4248 . 1 6 25 2 G H1 H 12.4721 . 1 7 25 2 G H1' H 5.6658 . 1 8 25 2 G H8 H 7.2424 . 1 9 25 2 G C8 C 139.2752 . 1 10 25 2 G N1 N 149.6525 . 1 11 26 3 G H1 H 12.1031 . 1 12 26 3 G H1' H 5.4961 . 1 13 26 3 G H8 H 6.9488 . 1 14 26 3 G C8 C 139.3044 . 1 15 26 3 G N1 N 149.0752 . 1 16 27 4 A H1' H 5.7127 . 1 17 27 4 A H2 H 7.5747 . 1 18 27 4 A H8 H 7.3922 . 1 19 27 4 A C1' C 90.4958 . 1 20 27 4 A C2 C 154.0868 . 1 21 27 4 A C8 C 140.8452 . 1 22 28 5 U H1' H 5.0378 . 1 23 28 5 U H3 H 11.6165 . 1 24 28 5 U H5 H 5.0445 . 1 25 28 5 U H6 H 7.2769 . 1 26 28 5 U C6 C 137.9798 . 1 27 28 5 U N3 N 157.1924 . 1 28 29 6 C H1' H 5.3303 . 1 29 29 6 C H5 H 5.3354 . 1 30 29 6 C H6 H 7.4815 . 1 31 29 6 C H41 H 8.1576 . 2 32 29 6 C H42 H 6.6078 . 2 33 30 7 U H1' H 5.2462 . 1 34 30 7 U H3 H 11.4164 . 1 35 30 7 U H5 H 5.1657 . 1 36 30 7 U H6 H 7.4095 . 1 37 30 7 U C6 C 139.1252 . 1 38 30 7 U N3 N 156.0179 . 1 39 31 8 G H1 H 12.2934 . 1 40 31 8 G H1' H 5.4659 . 1 41 31 8 G H8 H 7.5369 . 1 42 31 8 G C8 C 142.8938 . 1 43 31 8 G N1 N 150.9694 . 1 44 32 9 U H1' H 5.252 . 1 45 32 9 U H3 H 11.8566 . 1 46 32 9 U H5 H 5.1116 . 1 47 32 9 U H6 H 7.3914 . 1 48 32 9 U C6 C 143.8929 . 1 49 32 9 U N3 N 157.0322 . 1 50 33 10 C H1' H 5.3966 . 1 51 33 10 C H5 H 5.2755 . 1 52 33 10 C H6 H 7.534 . 1 53 33 10 C H41 H 8.0706 . 2 54 33 10 C H42 H 6.1764 . 2 55 34 11 A H1' H 5.5104 . 1 56 34 11 A H2 H 7.8334 . 1 57 34 11 A H8 H 7.8449 . 1 58 34 11 A C1' C 92.3246 . 1 59 34 11 A C2 C 153.9452 . 1 60 35 12 C H1' H 4.9316 . 1 61 35 12 C H5 H 4.9615 . 1 62 35 12 C H6 H 7.0538 . 1 63 35 12 C H41 H 8.2575 . 2 64 36 13 C H1' H 5.1622 . 1 65 36 13 C H5 H 5.2143 . 1 66 36 13 C H6 H 7.0722 . 1 67 36 13 C H41 H 7.961 . 2 68 36 13 C H42 H 6.8148 . 2 69 37 14 C H1' H 5.1305 . 1 70 37 14 C H5 H 5.19 . 1 71 37 14 C H6 H 7.5362 . 1 72 37 14 C H41 H 8.1844 . 2 73 37 14 C H42 H 6.8125 . 2 74 38 15 C H1' H 5.167 . 1 75 38 15 C H5 H 5.2135 . 1 76 38 15 C H6 H 7.3899 . 1 77 38 15 C H41 H 7.8601 . 2 78 38 15 C H42 H 6.5374 . 2 79 39 16 A H1' H 5.5294 . 1 80 39 16 A H8 H 7.6113 . 1 81 39 16 A C1' C 91.35 . 1 82 40 17 U H1' H 5.2515 . 1 83 40 17 U H3 H 11.4535 . 1 84 40 17 U H5 H 4.9687 . 1 85 40 17 U H6 H 7.082 . 1 86 40 17 U C5 C 110.2669 . 1 87 40 17 U N3 N 155.2704 . 1 88 41 18 U H1' H 5.3448 . 1 89 41 18 U H5 H 5.5019 . 1 90 41 18 U H6 H 7.5705 . 1 91 41 18 U C6 C 144.7306 . 1 92 42 19 G H1 H 12.2497 . 1 93 42 19 G H1' H 5.6301 . 1 94 42 19 G H8 H 7.5298 . 1 95 42 19 G C8 C 142.8186 . 1 96 42 19 G N1 N 149.3956 . 1 97 43 20 A H1' H 5.7559 . 1 98 43 20 A H2 H 7.5106 . 1 99 43 20 A H8 H 7.6011 . 1 100 43 20 A C1' C 92.0074 . 1 101 43 20 A C2 C 153.6798 . 1 102 44 21 U H1' H 5.2843 . 1 103 44 21 U H3 H 13.7981 . 1 104 44 21 U H5 H 4.7094 . 1 105 44 21 U H6 H 7.3566 . 1 106 44 21 U C6 C 142.1932 . 1 107 44 21 U N3 N 164.56 . 1 108 45 22 C H1' H 5.2957 . 1 109 45 22 C H5 H 5.3369 . 1 110 45 22 C H6 H 7.4444 . 1 111 45 22 C H41 H 8.0121 . 2 112 45 22 C H42 H 6.5254 . 2 113 46 23 G H1 H 12.3364 . 1 114 46 23 G H1' H 5.3269 . 1 115 46 23 G H8 H 7.2792 . 1 116 46 23 G C8 C 141.8718 . 1 117 46 23 G N1 N 149.9308 . 1 118 47 24 C H1' H 5.1673 . 1 119 47 24 C H5 H 4.9972 . 1 120 47 24 C H6 H 7.4423 . 1 121 47 24 C H41 H 8.2412 . 2 122 47 24 C H42 H 6.7269 . 2 123 48 25 C H1' H 5.4 . 1 124 48 25 C H5 H 5.1676 . 1 125 48 25 C H6 H 7.4573 . 1 126 48 25 C H41 H 8.0747 . 2 127 48 25 C H42 H 6.7245 . 2 128 49 26 G H1 H 10.1812 . 1 129 49 26 G H1' H 5.3775 . 1 130 49 26 G H8 H 7.2169 . 1 131 49 26 G C8 C 144.1548 . 1 132 49 26 G N1 N 145.845 . 1 133 50 27 A H1' H 5.4097 . 1 134 50 27 A H2 H 7.7841 . 1 135 50 27 A H8 H 7.9729 . 1 136 50 27 A C1' C 90.8949 . 1 137 50 27 A C2 C 154.0856 . 1 138 50 27 A C8 C 139.9638 . 1 139 51 28 G H1 H 10.2883 . 1 140 51 28 G H1' H 4.6683 . 1 141 51 28 G H8 H 7.371 . 1 142 51 28 G N1 N 148.5802 . 1 143 52 29 A H1' H 5.7998 . 1 144 52 29 A H2 H 7.9489 . 1 145 52 29 A H8 H 7.7433 . 1 146 52 29 A C1' C 90.2264 . 1 147 52 29 A C2 C 155.6568 . 1 148 52 29 A C8 C 138.3734 . 1 149 60 30 G H1 H 12.2206 . 1 150 60 30 G H1' H 4.9086 . 1 151 60 30 G H8 H 7.575 . 1 152 60 30 G C8 C 136.9167 . 1 153 60 30 G N1 N 148.3234 . 1 154 61 31 G H1 H 13.0162 . 1 155 61 31 G H1' H 5.5055 . 1 156 61 31 G H8 H 6.8827 . 1 157 61 31 G C8 C 139.1652 . 1 158 61 31 G N1 N 151.4777 . 1 159 62 32 C H1' H 5.4442 . 1 160 62 32 C H5 H 4.922 . 1 161 62 32 C H6 H 7.1558 . 1 162 62 32 C H41 H 8.3206 . 2 163 63 33 U H1' H 5.1672 . 1 164 63 33 U H3 H 10.3869 . 1 165 63 33 U H5 H 5.5031 . 1 166 63 33 U H6 H 7.5744 . 1 167 63 33 U C6 C 144.8848 . 1 168 63 33 U N3 N 151.4264 . 1 169 64 34 G H1 H 12.088 . 1 170 64 34 G H1' H 5.5018 . 1 171 64 34 G H8 H 7.4728 . 1 172 64 34 G C8 C 142.8691 . 1 173 64 34 G N1 N 149.0752 . 1 174 65 35 A H1' H 5.7491 . 1 175 65 35 A H2 H 7.4964 . 1 176 65 35 A H8 H 7.7171 . 1 177 65 35 A C1' C 92.0145 . 1 178 65 35 A C2 C 153.6798 . 1 179 66 36 U H1' H 5.2588 . 1 180 66 36 U H3 H 13.7957 . 1 181 66 36 U H5 H 4.6728 . 1 182 66 36 U H6 H 7.3466 . 1 183 66 36 U C6 C 140.2092 . 1 184 66 36 U N3 N 164.56 . 1 185 67 37 C H1' H 5.3071 . 1 186 67 37 C H5 H 5.1928 . 1 187 67 37 C H6 H 7.3672 . 1 188 67 37 C H41 H 8.0239 . 2 189 67 37 C H42 H 6.6134 . 2 190 68 38 U H1' H 5.2959 . 1 191 68 38 U H3 H 11.6766 . 1 192 68 38 U H5 H 5.2581 . 1 193 68 38 U H6 H 7.5055 . 1 194 68 38 U C6 C 139.9433 . 1 195 69 39 G H1 H 11.8504 . 1 196 69 39 G H1' H 5.4659 . 1 197 69 39 G H8 H 7.1024 . 1 198 69 39 G C8 C 139.1588 . 1 199 69 39 G N1 N 147.9787 . 1 200 70 40 G H1 H 12.578 . 1 201 70 40 G H1' H 5.5074 . 1 202 70 40 G H8 H 6.9525 . 1 203 70 40 G C8 C 139.3829 . 1 204 70 40 G N1 N 151.4162 . 1 205 71 41 RY H1' H 5.5886 . 1 206 71 41 RY H5 H 5.7422 . 1 207 71 41 RY H6 H 7.4264 . 1 208 71 41 RY H41 H 10.0661 . 2 209 71 41 RY H42 H 8.7392 . 2 210 72 42 U H1' H 5.7228 . 1 211 72 42 U H5 H 5.656 . 1 212 72 42 U H6 H 7.5503 . 1 213 72 42 U C6 C 144.8881 . 1 214 73 43 G H1 H 12.2701 . 1 215 73 43 G H1' H 5.5479 . 1 216 73 43 G H8 H 7.0674 . 1 217 73 43 G C8 C 141.2248 . 1 218 73 43 G N1 N 150.6154 . 1 219 74 44 G H1 H 12.9239 . 1 220 74 44 G H1' H 5.7133 . 1 221 74 44 G H8 H 7.2958 . 1 222 74 44 G C8 C 141.7694 . 1 223 74 44 G N1 N 150.9374 . 1 224 75 45 RY H1' H 5.6377 . 1 225 75 45 RY H5 H 5.3156 . 1 226 75 45 RY H6 H 7.3679 . 1 227 76 46 U H1' H 5.5579 . 1 228 76 46 U H5 H 5.4996 . 1 229 76 46 U H6 H 7.5008 . 1 230 76 46 U C6 C 143.8929 . 1 231 77 47 A H1' H 5.8363 . 1 232 77 47 A H8 H 8.0461 . 1 233 77 47 A C1' C 90.1865 . 1 234 77 47 A C8 C 140.0195 . 1 235 78 48 G H1 H 11.784 . 1 236 78 48 G H1' H 5.2757 . 1 237 78 48 G H8 H 7.5301 . 1 238 78 48 G C8 C 142.7426 . 1 239 78 48 G N1 N 148.132 . 1 240 79 49 G H1 H 11.1202 . 1 241 79 49 G H1' H 5.5027 . 1 242 79 49 G H8 H 7.1789 . 1 243 79 49 G C8 C 141.6886 . 1 244 79 49 G N1 N 144.4304 . 1 245 80 50 C H1' H 5.3278 . 1 246 80 50 C H5 H 5.0703 . 1 247 80 50 C H6 H 7.2925 . 1 248 80 50 C H41 H 8.0336 . 2 249 80 50 C H42 H 6.6768 . 2 250 81 51 G H1 H 10.3674 . 1 251 81 51 G H1' H 5.4721 . 1 252 81 51 G H8 H 7.1826 . 1 253 81 51 G C8 C 139.0745 . 1 254 81 51 G N1 N 139.8924 . 1 255 82 52 G H1 H 12.4808 . 1 256 82 52 G H1' H 5.4948 . 1 257 82 52 G H8 H 6.9746 . 1 258 82 52 G C8 C 139.6942 . 1 259 82 52 G N1 N 150.3218 . 1 260 83 53 G H1 H 11.2537 . 1 261 83 53 G H1' H 5.5106 . 1 262 83 53 G H8 H 6.8727 . 1 263 83 53 G C8 C 141.05 . 1 264 83 53 G N1 N 144.0567 . 1 265 84 54 U H1' H 5.2191 . 1 266 84 54 U H3 H 14.171 . 1 267 84 54 U H5 H 4.906 . 1 268 84 54 U H6 H 7.4986 . 1 269 84 54 U C6 C 139.8799 . 1 270 84 54 U N3 N 166.1083 . 1 271 85 55 C H1' H 5.2966 . 1 272 85 55 C H5 H 5.3329 . 1 273 85 55 C H6 H 7.6609 . 1 274 85 55 C H41 H 8.1661 . 2 275 85 55 C H42 H 6.7908 . 2 276 86 56 C H1' H 5.1979 . 1 277 86 56 C H5 H 5.2216 . 1 278 86 56 C H6 H 7.4752 . 1 279 86 56 C H41 H 7.9909 . 2 280 86 56 C H42 H 6.6426 . 2 281 87 57 C H1' H 5.4698 . 1 282 87 57 C H5 H 5.1692 . 1 283 87 57 C H6 H 7.3701 . 1 stop_ save_