data_28027 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; human_CIRBP_68-137 ; _BMRB_accession_number 28027 _BMRB_flat_file_name bmr28027.str _Entry_type original _Submission_date 2019-10-04 _Accession_date 2019-10-04 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bourgeois Benjamin . . 2 Madl Tobias . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 81 "15N chemical shifts" 81 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-04-21 update BMRB 'update entry citation' 2020-04-03 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 28025 'human CIRBP 138-172' 28026 'human TNPO1 328-381' stop_ _Original_release_date 2019-10-04 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Nonclassical nuclear localization signals mediate nuclear import of CIRBP ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 32234784 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bourgeois Benjamin . . 2 Hutten Saskia . . 3 Gottschalk Benjamin . . 4 Hofweber Mario . . 5 Richter Gesa . . 6 Sternat Julia . . 7 Abou-Ajram Claudia . . 8 Gobl Christoph . . 9 Leitinger Gerd . . 10 Graier Wolfgang F. . 11 Dormann Dorothee . . 12 Madl Tobias . . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_name_full 'Proceedings of the National Academy of Sciences of the United States of America' _Journal_volume 117 _Journal_issue 15 _Journal_ISSN 1091-6490 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 8503 _Page_last 8514 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name human_CIRBP_38-137 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label CIRBP_68-137 $CIRBP stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_CIRBP _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common CIRBP _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 98 _Mol_residue_sequence ; MKHHHHHHPMSDYDIPTTEN LYFQGAMGNGKSVDGRQIRV DQAGKSSDNRSRGYRGGSAG GRGFFRGGRGRGRGFSRGGG DRGYGGNRFESRSGGYGG ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 40 MET 2 41 LYS 3 42 HIS 4 43 HIS 5 44 HIS 6 45 HIS 7 46 HIS 8 47 HIS 9 48 PRO 10 49 MET 11 50 SER 12 51 ASP 13 52 TYR 14 53 ASP 15 54 ILE 16 55 PRO 17 56 THR 18 57 THR 19 58 GLU 20 59 ASN 21 60 LEU 22 61 TYR 23 62 PHE 24 63 GLN 25 64 GLY 26 65 ALA 27 66 MET 28 67 GLY 29 68 ASN 30 69 GLY 31 70 LYS 32 71 SER 33 72 VAL 34 73 ASP 35 74 GLY 36 75 ARG 37 76 GLN 38 77 ILE 39 78 ARG 40 79 VAL 41 80 ASP 42 81 GLN 43 82 ALA 44 83 GLY 45 84 LYS 46 85 SER 47 86 SER 48 87 ASP 49 88 ASN 50 89 ARG 51 90 SER 52 91 ARG 53 92 GLY 54 93 TYR 55 94 ARG 56 95 GLY 57 96 GLY 58 97 SER 59 98 ALA 60 99 GLY 61 100 GLY 62 101 ARG 63 102 GLY 64 103 PHE 65 104 PHE 66 105 ARG 67 106 GLY 68 107 GLY 69 108 ARG 70 109 GLY 71 110 ARG 72 111 GLY 73 112 ARG 74 113 GLY 75 114 PHE 76 115 SER 77 116 ARG 78 117 GLY 79 118 GLY 80 119 GLY 81 120 ASP 82 121 ARG 83 122 GLY 84 123 TYR 85 124 GLY 86 125 GLY 87 126 ASN 88 127 ARG 89 128 PHE 90 129 GLU 91 130 SER 92 131 ARG 93 132 SER 94 133 GLY 95 134 GLY 96 135 TYR 97 136 GLY 98 137 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $CIRBP Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $CIRBP 'recombinant technology' . Escherichia coli . pETM-11 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $CIRBP 500 uM '[U-99% 13C; U-99% 15N]' D2O 10 % '[U-100% 2H]' TRIS 50 mM 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' TCEP 2 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CCPN _Saveframe_category software _Name CCPN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . CCPN . . 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task collection 'peak picking' processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HN(CA)NNH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)NNH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.15 . M pH 6.7 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.707 na indirect . . . 1 '[15N] ammonium chloride' N 15 nitrogen ppm 117 na indirect . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D CBCA(CO)NH' '3D HN(CA)NNH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name CIRBP_68-137 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 49 10 MET H H 8.648 . . 2 49 10 MET N N 120.302 . . 3 50 11 SER H H 8.189 . . 4 50 11 SER N N 116.090 . . 5 51 12 ASP H H 8.249 . . 6 51 12 ASP N N 121.779 . . 7 52 13 TYR H H 7.823 . . 8 52 13 TYR N N 118.789 . . 9 53 14 ASP H H 8.071 . . 10 53 14 ASP N N 121.599 . . 11 54 15 ILE H H 7.839 . . 12 54 15 ILE N N 122.175 . . 13 56 17 THR H H 8.220 . . 14 56 17 THR N N 114.495 . . 15 57 18 THR H H 8.015 . . 16 57 18 THR N N 115.235 . . 17 58 19 GLU H H 8.307 . . 18 58 19 GLU N N 122.636 . . 19 59 20 ASN H H 8.301 . . 20 59 20 ASN N N 119.338 . . 21 60 21 LEU H H 7.959 . . 22 60 21 LEU N N 122.128 . . 23 61 22 TYR H H 7.894 . . 24 61 22 TYR N N 119.485 . . 25 62 23 PHE H H 7.906 . . 26 62 23 PHE N N 121.297 . . 27 63 24 GLN H H 7.670 . . 28 63 24 GLN N N 126.010 . . 29 66 27 MET H H 8.476 . . 30 66 27 MET N N 119.232 . . 31 67 28 GLY H H 8.258 . . 32 67 28 GLY N N 110.037 . . 33 68 29 ASN H H 7.906 . . 34 68 29 ASN N N 124.123 . . 35 69 30 GLY H H 8.213 . . 36 69 30 GLY N N 113.905 . . 37 70 31 LYS H H 8.195 . . 38 70 31 LYS N N 120.365 . . 39 71 32 SER H H 8.308 . . 40 71 32 SER N N 117.006 . . 41 72 33 VAL H H 8.190 . . 42 72 33 VAL N N 121.310 . . 43 73 34 ASP H H 8.265 . . 44 73 34 ASP N N 123.069 . . 45 74 35 GLY H H 8.279 . . 46 74 35 GLY N N 109.462 . . 47 75 36 ARG H H 8.022 . . 48 75 36 ARG N N 120.063 . . 49 76 37 GLN H H 8.315 . . 50 76 37 GLN N N 121.028 . . 51 77 38 ILE H H 8.098 . . 52 77 38 ILE N N 122.600 . . 53 78 39 ARG H H 8.337 . . 54 78 39 ARG N N 125.612 . . 55 79 40 VAL H H 8.209 . . 56 79 40 VAL N N 121.835 . . 57 80 41 ASP H H 8.387 . . 58 80 41 ASP N N 123.549 . . 59 81 42 GLN H H 8.242 . . 60 81 42 GLN N N 120.755 . . 61 82 43 ALA H H 8.230 . . 62 82 43 ALA N N 124.498 . . 63 83 44 GLY H H 8.259 . . 64 83 44 GLY N N 107.954 . . 65 84 45 LYS H H 8.060 . . 66 84 45 LYS N N 120.622 . . 67 85 46 SER H H 8.342 . . 68 85 46 SER N N 116.969 . . 69 86 47 SER H H 8.300 . . 70 86 47 SER N N 117.556 . . 71 87 48 ASP H H 8.205 . . 72 87 48 ASP N N 122.030 . . 73 88 49 ASN H H 8.293 . . 74 88 49 ASN N N 119.499 . . 75 89 50 ARG H H 8.208 . . 76 89 50 ARG N N 120.641 . . 77 90 51 SER H H 8.188 . . 78 90 51 SER N N 116.020 . . 79 91 52 ARG H H 8.198 . . 80 91 52 ARG N N 122.450 . . 81 92 53 GLY H H 8.223 . . 82 92 53 GLY N N 109.068 . . 83 93 54 TYR H H 7.979 . . 84 93 54 TYR N N 120.484 . . 85 94 55 ARG H H 8.261 . . 86 94 55 ARG N N 124.047 . . 87 95 56 GLY H H 7.650 . . 88 95 56 GLY N N 108.926 . . 89 96 57 GLY H H 8.149 . . 90 96 57 GLY N N 108.569 . . 91 97 58 SER H H 8.213 . . 92 97 58 SER N N 115.770 . . 93 98 59 ALA H H 8.342 . . 94 98 59 ALA N N 125.842 . . 95 99 60 GLY H H 8.187 . . 96 99 60 GLY N N 107.763 . . 97 100 61 GLY H H 8.115 . . 98 100 61 GLY N N 108.465 . . 99 101 62 ARG H H 8.152 . . 100 101 62 ARG N N 120.141 . . 101 102 63 GLY H H 8.300 . . 102 102 63 GLY N N 109.328 . . 103 103 64 PHE H H 7.927 . . 104 103 64 PHE N N 119.893 . . 105 104 65 PHE H H 8.167 . . 106 104 65 PHE N N 122.011 . . 107 105 66 ARG H H 8.190 . . 108 105 66 ARG N N 123.553 . . 109 106 67 GLY H H 7.815 . . 110 106 67 GLY N N 109.218 . . 111 107 68 GLY H H 8.133 . . 112 107 68 GLY N N 108.516 . . 113 113 74 GLY H H 8.338 . . 114 113 74 GLY N N 109.570 . . 115 114 75 PHE H H 8.001 . . 116 114 75 PHE N N 119.821 . . 117 115 76 SER H H 8.190 . . 118 115 76 SER N N 117.400 . . 119 116 77 ARG H H 8.317 . . 120 116 77 ARG N N 123.065 . . 121 117 78 GLY H H 8.346 . . 122 117 78 GLY N N 109.806 . . 123 118 79 GLY H H 8.205 . . 124 118 79 GLY N N 108.703 . . 125 119 80 GLY H H 8.242 . . 126 119 80 GLY N N 108.791 . . 127 120 81 ASP H H 8.199 . . 128 120 81 ASP N N 120.331 . . 129 121 82 ARG H H 8.248 . . 130 121 82 ARG N N 120.821 . . 131 122 83 GLY H H 8.251 . . 132 122 83 GLY N N 108.828 . . 133 123 84 TYR H H 8.016 . . 134 123 84 TYR N N 119.980 . . 135 124 85 GLY H H 8.371 . . 136 124 85 GLY N N 111.072 . . 137 125 86 GLY H H 7.849 . . 138 125 86 GLY N N 108.220 . . 139 126 87 ASN H H 8.237 . . 140 126 87 ASN N N 118.414 . . 141 127 88 ARG H H 8.178 . . 142 127 88 ARG N N 121.076 . . 143 128 89 PHE H H 8.153 . . 144 128 89 PHE N N 120.340 . . 145 129 90 GLU H H 8.166 . . 146 129 90 GLU N N 122.005 . . 147 130 91 SER H H 8.226 . . 148 130 91 SER N N 116.875 . . 149 131 92 ARG H H 8.337 . . 150 131 92 ARG N N 122.995 . . 151 132 93 SER H H 8.277 . . 152 132 93 SER N N 116.607 . . 153 133 94 GLY H H 8.327 . . 154 133 94 GLY N N 110.822 . . 155 134 95 GLY H H 8.107 . . 156 134 95 GLY N N 108.365 . . 157 135 96 TYR H H 8.085 . . 158 135 96 TYR N N 120.193 . . 159 136 97 GLY H H 8.324 . . 160 136 97 GLY N N 111.706 . . 161 137 98 GLY H H 7.486 . . 162 137 98 GLY N N 114.795 . . stop_ save_