data_27998 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural basis for client recognition and activity of Hsp40 chaperones ; _BMRB_accession_number 27998 _BMRB_flat_file_name bmr27998.str _Entry_type original _Submission_date 2019-08-16 _Accession_date 2019-08-16 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jiang Yajun . . 2 Rossi Paolo . . 3 Kalodimos Charalampos G. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 148 "15N chemical shifts" 148 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-11-07 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 27999 'Sis1 SBD' 28000 'Ydj1 SBD' 28001 'CbpA SBD' stop_ _Original_release_date 2019-08-19 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural basis for client recognition and activity of Hsp40 chaperones ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31604242 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jiang Yajun . . 2 Rossi Paolo . . 3 Kalodimos Charalampos G. . stop_ _Journal_abbreviation Science _Journal_volume 365 _Journal_issue 6459 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1313 _Page_last 1319 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name DnaJB1_SBD _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label DnaJB1_SBD $DnaJB1_SBD stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_DnaJB1_SBD _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common DnaJB1_SBD _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function chaperone stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 185 _Mol_residue_sequence ; ARKKQDPPVTHDLRVSLEEI YSGCTKKMKISHKRLNPDGK SIRNEDKILTIEVKKGWKEG TKITFPKEGDQTSNNIPADI VFVLKDKPHNIFKRDGSDVI YPARISLREALCGCTVNVPT LDGRTIPVVFKDVIRPGMRR KVPGEGLPLPKTPEKRGDLI IEFEVIFPERIPQTSRTVLE QVLPI ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 156 ALA 2 157 ARG 3 158 LYS 4 159 LYS 5 160 GLN 6 161 ASP 7 162 PRO 8 163 PRO 9 164 VAL 10 165 THR 11 166 HIS 12 167 ASP 13 168 LEU 14 169 ARG 15 170 VAL 16 171 SER 17 172 LEU 18 173 GLU 19 174 GLU 20 175 ILE 21 176 TYR 22 177 SER 23 178 GLY 24 179 CYS 25 180 THR 26 181 LYS 27 182 LYS 28 183 MET 29 184 LYS 30 185 ILE 31 186 SER 32 187 HIS 33 188 LYS 34 189 ARG 35 190 LEU 36 191 ASN 37 192 PRO 38 193 ASP 39 194 GLY 40 195 LYS 41 196 SER 42 197 ILE 43 198 ARG 44 199 ASN 45 200 GLU 46 201 ASP 47 202 LYS 48 203 ILE 49 204 LEU 50 205 THR 51 206 ILE 52 207 GLU 53 208 VAL 54 209 LYS 55 210 LYS 56 211 GLY 57 212 TRP 58 213 LYS 59 214 GLU 60 215 GLY 61 216 THR 62 217 LYS 63 218 ILE 64 219 THR 65 220 PHE 66 221 PRO 67 222 LYS 68 223 GLU 69 224 GLY 70 225 ASP 71 226 GLN 72 227 THR 73 228 SER 74 229 ASN 75 230 ASN 76 231 ILE 77 232 PRO 78 233 ALA 79 234 ASP 80 235 ILE 81 236 VAL 82 237 PHE 83 238 VAL 84 239 LEU 85 240 LYS 86 241 ASP 87 242 LYS 88 243 PRO 89 244 HIS 90 245 ASN 91 246 ILE 92 247 PHE 93 248 LYS 94 249 ARG 95 250 ASP 96 251 GLY 97 252 SER 98 253 ASP 99 254 VAL 100 255 ILE 101 256 TYR 102 257 PRO 103 258 ALA 104 259 ARG 105 260 ILE 106 261 SER 107 262 LEU 108 263 ARG 109 264 GLU 110 265 ALA 111 266 LEU 112 267 CYS 113 268 GLY 114 269 CYS 115 270 THR 116 271 VAL 117 272 ASN 118 273 VAL 119 274 PRO 120 275 THR 121 276 LEU 122 277 ASP 123 278 GLY 124 279 ARG 125 280 THR 126 281 ILE 127 282 PRO 128 283 VAL 129 284 VAL 130 285 PHE 131 286 LYS 132 287 ASP 133 288 VAL 134 289 ILE 135 290 ARG 136 291 PRO 137 292 GLY 138 293 MET 139 294 ARG 140 295 ARG 141 296 LYS 142 297 VAL 143 298 PRO 144 299 GLY 145 300 GLU 146 301 GLY 147 302 LEU 148 303 PRO 149 304 LEU 150 305 PRO 151 306 LYS 152 307 THR 153 308 PRO 154 309 GLU 155 310 LYS 156 311 ARG 157 312 GLY 158 313 ASP 159 314 LEU 160 315 ILE 161 316 ILE 162 317 GLU 163 318 PHE 164 319 GLU 165 320 VAL 166 321 ILE 167 322 PHE 168 323 PRO 169 324 GLU 170 325 ARG 171 326 ILE 172 327 PRO 173 328 GLN 174 329 THR 175 330 SER 176 331 ARG 177 332 THR 178 333 VAL 179 334 LEU 180 335 GLU 181 336 GLN 182 337 VAL 183 338 LEU 184 339 PRO 185 340 ILE stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $DnaJB1_SBD Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $DnaJB1_SBD 'recombinant technology' . Escherichia coli . pet16b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DnaJB1_SBD 400 uM '[U-13C; U-15N; U-2H]' 'potassium phosphate' 20 mM 'natural abundance' 'potassium chloride' 75 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_PINE _Saveframe_category software _Name PINE _Version . loop_ _Vendor _Address _Electronic_address 'Bahrami, Markley, Assadi, and Eghbalnia' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCOCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCOCACB' _Sample_label $sample_1 save_ save_3D_HNCO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCACO_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACO' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 7 . pH pressure 1 . atm temperature 305 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 DSS P 31 'methyl protons' ppm 0.00 na indirect . . . 0.404808636 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name DnaJB1_SBD _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 158 3 LYS H H 8.143 0.00 . 2 158 3 LYS N N 123.358 0.00 . 3 159 4 LYS H H 8.211 0.00 . 4 159 4 LYS N N 122.943 0.00 . 5 160 5 GLN H H 8.475 0.00 . 6 160 5 GLN N N 124.432 0.00 . 7 161 6 ASP H H 7.887 0.00 . 8 161 6 ASP N N 129.276 0.00 . 9 164 9 VAL H H 8.328 0.00 . 10 164 9 VAL N N 124.989 0.00 . 11 165 10 THR H H 7.877 0.00 . 12 165 10 THR N N 120.521 0.00 . 13 166 11 HIS H H 8.845 0.00 . 14 166 11 HIS N N 124.700 0.00 . 15 167 12 ASP H H 8.737 0.00 . 16 167 12 ASP N N 123.277 0.00 . 17 168 13 LEU H H 8.919 0.00 . 18 168 13 LEU N N 126.421 0.00 . 19 169 14 ARG H H 8.688 0.00 . 20 169 14 ARG N N 126.773 0.00 . 21 170 15 VAL H H 8.734 0.00 . 22 170 15 VAL N N 120.349 0.00 . 23 171 16 SER H H 9.502 0.00 . 24 171 16 SER N N 124.052 0.00 . 25 172 17 LEU H H 9.320 0.00 . 26 172 17 LEU N N 123.374 0.00 . 27 176 21 TYR H H 8.242 0.00 . 28 176 21 TYR N N 121.546 0.00 . 29 177 22 SER H H 7.651 0.00 . 30 177 22 SER N N 108.590 0.00 . 31 178 23 GLY H H 7.771 0.00 . 32 178 23 GLY N N 113.388 0.00 . 33 179 24 CYS H H 8.433 0.00 . 34 179 24 CYS N N 112.854 0.00 . 35 180 25 THR H H 7.842 0.58 . 36 180 25 THR N N 121.678 5.07 . 37 181 26 LYS H H 9.136 0.00 . 38 181 26 LYS N N 126.565 0.00 . 39 182 27 LYS H H 8.328 0.00 . 40 182 27 LYS N N 122.620 0.00 . 41 183 28 MET H H 8.634 0.00 . 42 183 28 MET N N 123.214 0.00 . 43 184 29 LYS H H 8.439 0.00 . 44 184 29 LYS N N 126.480 0.00 . 45 185 30 ILE H H 8.922 0.00 . 46 185 30 ILE N N 123.414 0.00 . 47 186 31 SER H H 8.181 0.00 . 48 186 31 SER N N 117.323 0.00 . 49 187 32 HIS H H 9.001 0.00 . 50 187 32 HIS N N 121.588 0.00 . 51 188 33 LYS H H 8.770 0.00 . 52 188 33 LYS N N 123.627 0.00 . 53 189 34 ARG H H 9.003 0.00 . 54 189 34 ARG N N 123.484 0.00 . 55 190 35 LEU H H 8.037 0.00 . 56 190 35 LEU N N 123.003 0.00 . 57 191 36 ASN H H 8.613 0.00 . 58 191 36 ASN N N 124.208 0.00 . 59 193 38 ASP H H 7.540 0.00 . 60 193 38 ASP N N 114.406 0.00 . 61 194 39 GLY H H 8.360 0.00 . 62 194 39 GLY N N 108.989 0.00 . 63 195 40 LYS H H 8.538 0.00 . 64 195 40 LYS N N 119.452 0.00 . 65 196 41 SER H H 8.906 0.00 . 66 196 41 SER N N 117.512 0.00 . 67 197 42 ILE H H 8.214 0.00 . 68 197 42 ILE N N 119.353 0.00 . 69 198 43 ARG H H 8.963 0.00 . 70 198 43 ARG N N 124.384 0.00 . 71 199 44 ASN H H 8.594 0.00 . 72 199 44 ASN N N 121.267 0.00 . 73 200 45 GLU H H 8.770 0.00 . 74 200 45 GLU N N 124.468 0.00 . 75 201 46 ASP H H 8.413 0.00 . 76 201 46 ASP N N 123.953 0.00 . 77 202 47 LYS H H 8.843 0.00 . 78 202 47 LYS N N 123.215 0.00 . 79 203 48 ILE H H 8.310 0.00 . 80 203 48 ILE N N 124.443 0.00 . 81 204 49 LEU H H 8.876 0.00 . 82 204 49 LEU N N 130.165 0.00 . 83 205 50 THR H H 8.336 0.00 . 84 205 50 THR N N 119.369 0.00 . 85 206 51 ILE H H 9.080 0.00 . 86 206 51 ILE N N 128.254 0.00 . 87 207 52 GLU H H 8.512 0.00 . 88 207 52 GLU N N 128.693 0.00 . 89 208 53 VAL H H 8.645 0.00 . 90 208 53 VAL N N 127.381 0.00 . 91 210 55 LYS H H 7.629 0.00 . 92 210 55 LYS N N 116.683 0.00 . 93 211 56 GLY H H 8.688 0.00 . 94 211 56 GLY N N 106.096 0.00 . 95 212 57 TRP H H 7.450 0.00 . 96 212 57 TRP N N 121.858 0.00 . 97 215 60 GLY H H 9.310 0.00 . 98 215 60 GLY N N 115.411 0.00 . 99 216 61 THR H H 7.504 0.00 . 100 216 61 THR N N 119.563 0.00 . 101 217 62 LYS H H 8.214 0.00 . 102 217 62 LYS N N 126.367 0.00 . 103 218 63 ILE H H 9.353 0.00 . 104 218 63 ILE N N 126.602 0.00 . 105 219 64 THR H H 8.390 0.00 . 106 219 64 THR N N 123.446 0.00 . 107 220 65 PHE H H 9.817 0.00 . 108 220 65 PHE N N 128.618 0.00 . 109 222 67 LYS H H 9.432 0.00 . 110 222 67 LYS N N 117.615 0.00 . 111 223 68 GLU H H 6.763 0.00 . 112 223 68 GLU N N 116.575 0.00 . 113 224 69 GLY H H 9.237 0.00 . 114 224 69 GLY N N 109.417 0.00 . 115 225 70 ASP H H 8.801 0.00 . 116 225 70 ASP N N 119.185 0.00 . 117 226 71 GLN H H 8.381 0.00 . 118 226 71 GLN N N 124.039 0.00 . 119 227 72 THR H H 8.123 0.00 . 120 227 72 THR N N 113.195 0.00 . 121 230 75 ASN H H 7.633 0.00 . 122 230 75 ASN N N 119.133 0.00 . 123 231 76 ILE H H 8.138 0.00 . 124 231 76 ILE N N 122.138 0.00 . 125 233 78 ALA H H 8.723 0.00 . 126 233 78 ALA N N 123.167 0.00 . 127 234 79 ASP H H 8.950 0.00 . 128 234 79 ASP N N 120.946 0.00 . 129 235 80 ILE H H 7.997 0.00 . 130 235 80 ILE N N 120.751 0.00 . 131 236 81 VAL H H 8.903 0.00 . 132 236 81 VAL N N 129.374 0.00 . 133 237 82 PHE H H 9.363 0.00 . 134 237 82 PHE N N 125.227 0.00 . 135 238 83 VAL H H 8.694 0.00 . 136 238 83 VAL N N 122.235 0.00 . 137 239 84 LEU H H 8.709 0.00 . 138 239 84 LEU N N 129.654 0.00 . 139 240 85 LYS H H 8.779 0.00 . 140 240 85 LYS N N 125.816 0.00 . 141 241 86 ASP H H 9.391 0.00 . 142 241 86 ASP N N 123.814 0.00 . 143 242 87 LYS H H 8.352 0.00 . 144 242 87 LYS N N 126.443 0.00 . 145 248 93 LYS H H 8.864 0.00 . 146 248 93 LYS N N 118.545 0.00 . 147 249 94 ARG H H 8.997 0.00 . 148 249 94 ARG N N 125.758 0.00 . 149 250 95 ASP H H 8.585 0.00 . 150 250 95 ASP N N 127.809 0.00 . 151 251 96 GLY H H 8.882 0.00 . 152 251 96 GLY N N 114.514 0.00 . 153 252 97 SER H H 9.202 0.00 . 154 252 97 SER N N 125.372 0.00 . 155 253 98 ASP H H 8.081 0.00 . 156 253 98 ASP N N 123.207 0.00 . 157 254 99 VAL H H 8.250 0.00 . 158 254 99 VAL N N 113.870 0.00 . 159 255 100 ILE H H 9.288 0.00 . 160 255 100 ILE N N 125.515 0.00 . 161 256 101 TYR H H 9.585 0.00 . 162 256 101 TYR N N 129.171 0.00 . 163 258 103 ALA H H 7.887 0.00 . 164 258 103 ALA N N 129.276 0.00 . 165 259 104 ARG H H 8.135 0.00 . 166 259 104 ARG N N 124.012 0.00 . 167 260 105 ILE H H 8.636 0.00 . 168 260 105 ILE N N 119.637 0.00 . 169 261 106 SER H H 8.856 0.00 . 170 261 106 SER N N 117.809 0.00 . 171 262 107 LEU H H 7.640 0.00 . 172 262 107 LEU N N 121.256 0.00 . 173 263 108 ARG H H 8.667 0.00 . 174 263 108 ARG N N 118.754 0.00 . 175 264 109 GLU H H 7.688 0.00 . 176 264 109 GLU N N 119.600 0.00 . 177 265 110 ALA H H 8.145 0.00 . 178 265 110 ALA N N 120.548 0.00 . 179 266 111 LEU H H 7.696 0.00 . 180 266 111 LEU N N 111.702 0.00 . 181 267 112 CYS H H 8.131 0.00 . 182 267 112 CYS N N 113.303 0.00 . 183 268 113 GLY H H 7.620 0.00 . 184 268 113 GLY N N 114.058 0.00 . 185 269 114 CYS H H 8.237 0.00 . 186 269 114 CYS N N 114.948 0.00 . 187 270 115 THR H H 8.451 0.00 . 188 270 115 THR N N 117.957 0.00 . 189 271 116 VAL H H 9.331 0.00 . 190 271 116 VAL N N 128.567 0.00 . 191 272 117 ASN H H 8.814 0.00 . 192 272 117 ASN N N 127.732 0.00 . 193 273 118 VAL H H 8.734 0.00 . 194 273 118 VAL N N 129.165 0.00 . 195 275 120 THR H H 7.530 0.00 . 196 275 120 THR N N 103.673 0.00 . 197 276 121 LEU H H 9.136 0.00 . 198 276 121 LEU N N 121.805 0.00 . 199 277 122 ASP H H 7.967 0.00 . 200 277 122 ASP N N 113.026 0.00 . 201 278 123 GLY H H 7.513 0.00 . 202 278 123 GLY N N 107.553 0.00 . 203 279 124 ARG H H 7.446 0.00 . 204 279 124 ARG N N 119.960 0.00 . 205 280 125 THR H H 7.890 0.00 . 206 280 125 THR N N 114.006 0.00 . 207 281 126 ILE H H 9.353 0.00 . 208 281 126 ILE N N 126.602 0.00 . 209 283 128 VAL H H 9.098 0.00 . 210 283 128 VAL N N 128.907 0.00 . 211 284 129 VAL H H 8.142 0.00 . 212 284 129 VAL N N 127.984 0.00 . 213 285 130 PHE H H 9.314 0.00 . 214 285 130 PHE N N 127.363 0.00 . 215 287 132 ASP H H 7.760 0.00 . 216 287 132 ASP N N 120.365 0.00 . 217 288 133 VAL H H 7.673 0.00 . 218 288 133 VAL N N 119.241 0.00 . 219 289 134 ILE H H 8.576 0.00 . 220 289 134 ILE N N 131.695 0.00 . 221 290 135 ARG H H 6.258 0.00 . 222 290 135 ARG N N 120.738 0.00 . 223 292 137 GLY H H 8.349 0.00 . 224 292 137 GLY N N 114.051 0.00 . 225 293 138 MET H H 7.872 0.00 . 226 293 138 MET N N 121.793 0.00 . 227 294 139 ARG H H 8.318 0.00 . 228 294 139 ARG N N 122.946 0.00 . 229 296 141 LYS H H 8.952 0.00 . 230 296 141 LYS N N 126.333 0.00 . 231 297 142 VAL H H 9.886 0.00 . 232 297 142 VAL N N 128.192 0.00 . 233 299 144 GLY H H 8.909 0.00 . 234 299 144 GLY N N 110.584 0.00 . 235 300 145 GLU H H 6.377 0.00 . 236 300 145 GLU N N 114.687 0.00 . 237 301 146 GLY H H 7.929 0.00 . 238 301 146 GLY N N 108.367 0.00 . 239 302 147 LEU H H 8.567 0.00 . 240 302 147 LEU N N 121.639 0.00 . 241 304 149 LEU H H 8.251 0.00 . 242 304 149 LEU N N 127.491 0.00 . 243 309 154 GLU H H 9.036 0.00 . 244 309 154 GLU N N 119.959 0.00 . 245 310 155 LYS H H 8.464 0.00 . 246 310 155 LYS N N 123.480 0.00 . 247 311 156 ARG H H 8.076 0.00 . 248 311 156 ARG N N 118.630 0.00 . 249 312 157 GLY H H 8.564 0.00 . 250 312 157 GLY N N 107.026 0.00 . 251 313 158 ASP H H 9.216 0.00 . 252 313 158 ASP N N 119.368 0.00 . 253 314 159 LEU H H 7.591 0.00 . 254 314 159 LEU N N 122.535 0.00 . 255 315 160 ILE H H 9.651 0.00 . 256 315 160 ILE N N 128.521 0.00 . 257 316 161 ILE H H 9.110 0.00 . 258 316 161 ILE N N 129.439 0.00 . 259 317 162 GLU H H 8.070 0.00 . 260 317 162 GLU N N 128.074 0.00 . 261 318 163 PHE H H 8.512 0.00 . 262 318 163 PHE N N 125.519 0.00 . 263 319 164 GLU H H 8.973 0.00 . 264 319 164 GLU N N 126.464 0.00 . 265 320 165 VAL H H 8.598 0.00 . 266 320 165 VAL N N 125.691 0.00 . 267 321 166 ILE H H 9.423 0.00 . 268 321 166 ILE N N 130.009 0.00 . 269 322 167 PHE H H 9.466 0.00 . 270 322 167 PHE N N 133.648 0.00 . 271 324 169 GLU H H 8.613 0.00 . 272 324 169 GLU N N 119.605 0.00 . 273 325 170 ARG H H 7.515 0.00 . 274 325 170 ARG N N 114.058 0.00 . 275 326 171 ILE H H 8.776 0.00 . 276 326 171 ILE N N 121.771 0.00 . 277 330 175 SER H H 7.105 0.00 . 278 330 175 SER N N 116.447 0.00 . 279 331 176 ARG H H 8.051 0.00 . 280 331 176 ARG N N 123.601 0.00 . 281 332 177 THR H H 7.951 0.00 . 282 332 177 THR N N 112.898 0.00 . 283 333 178 VAL H H 6.840 0.00 . 284 333 178 VAL N N 120.791 0.00 . 285 334 179 LEU H H 8.166 0.00 . 286 334 179 LEU N N 120.617 0.00 . 287 335 180 GLU H H 7.891 0.00 . 288 335 180 GLU N N 116.674 0.00 . 289 336 181 GLN H H 7.248 0.00 . 290 336 181 GLN N N 114.398 0.00 . 291 337 182 VAL H H 7.604 0.00 . 292 337 182 VAL N N 111.404 0.00 . 293 338 183 LEU H H 7.777 0.00 . 294 338 183 LEU N N 123.244 0.00 . 295 340 185 ILE H H 7.562 0.00 . 296 340 185 ILE N N 122.975 0.00 . stop_ save_