data_27972
#######################
# Entry information #
#######################
save_entry_information
_Saveframe_category entry_information
_Entry_title
;
VSV Phosphoprotein 35-106
;
_BMRB_accession_number 27972
_BMRB_flat_file_name bmr27972.str
_Entry_type original
_Submission_date 2019-07-12
_Accession_date 2019-07-12
_Entry_origination author
_NMR_STAR_version 2.1.1
_Experimental_method NMR
_Details 'Chemical shift data for VSV Phosphoprotein Residues 35 - 106'
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Petit Chad M. .
2 Green Todd J. .
stop_
loop_
_Saveframe_category_type
_Saveframe_category_type_count
assigned_chemical_shifts 1
stop_
loop_
_Data_type
_Data_type_count
"1H chemical shifts" 60
"13C chemical shifts" 114
"15N chemical shifts" 60
stop_
loop_
_Revision_date
_Revision_keyword
_Revision_author
_Revision_detail
2020-03-31 update BMRB 'update entry citation'
2020-02-11 original author 'original release'
stop_
_Original_release_date 2019-07-12
save_
#############################
# Citation for this entry #
#############################
save_entry_citation
_Saveframe_category entry_citation
_Citation_full .
_Citation_title
;
The Connector Domain of Vesicular Stomatitis Virus Large Protein Interacts with the Viral Phosphoprotein
;
_Citation_status published
_Citation_type journal
_CAS_abstract_code .
_MEDLINE_UI_code .
_PubMed_ID 31896592
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Gould Joseph R. .
2 Qiu Shihong . .
3 Shang Qiao . .
4 Ogino Tomoaki . .
5 Prevelige Peter E. Jr
6 Petit Chad M. .
7 Green Todd J. .
stop_
_Journal_abbreviation 'J. Virol.'
_Journal_name_full 'Journal of virology'
_Journal_volume 94
_Journal_issue 6
_Journal_ISSN 1098-5514
_Journal_CSD .
_Book_chapter_title .
_Book_volume .
_Book_series .
_Book_ISBN .
_Conference_state_province .
_Conference_abstract_number .
_Page_first e01729-19
_Page_last e01729-19
_Year 2020
_Details .
save_
##################################
# Molecular system description #
##################################
save_assembly
_Saveframe_category molecular_system
_Mol_system_name 'VSV Phosphoprotein 35-106'
_Enzyme_commission_number .
loop_
_Mol_system_component_name
_Mol_label
'VSV Phosphoprotein 35-106' $L_Protein
stop_
_System_molecular_weight .
_System_physical_state native
_System_oligomer_state ?
_System_paramagnetic no
_System_thiol_state .
_Database_query_date .
_Details .
save_
########################
# Monomeric polymers #
########################
save_L_Protein
_Saveframe_category monomeric_polymer
_Mol_type polymer
_Mol_polymer_class protein
_Name_common L_Protein
_Molecular_mass .
_Mol_thiol_state 'all free'
_Details .
##############################
# Polymer residue sequence #
##############################
_Residue_count 72
_Mol_residue_sequence
;
SNYELFQEDGVEEHTRPSYF
QAADDSDTESEPEIEDNQGL
YVPDPEAEQVEGFIQGPLDD
YADEDVDVVFTS
;
loop_
_Residue_seq_code
_Residue_label
1 SER 2 ASN 3 TYR 4 GLU 5 LEU
6 PHE 7 GLN 8 GLU 9 ASP 10 GLY
11 VAL 12 GLU 13 GLU 14 HIS 15 THR
16 ARG 17 PRO 18 SER 19 TYR 20 PHE
21 GLN 22 ALA 23 ALA 24 ASP 25 ASP
26 SER 27 ASP 28 THR 29 GLU 30 SER
31 GLU 32 PRO 33 GLU 34 ILE 35 GLU
36 ASP 37 ASN 38 GLN 39 GLY 40 LEU
41 TYR 42 VAL 43 PRO 44 ASP 45 PRO
46 GLU 47 ALA 48 GLU 49 GLN 50 VAL
51 GLU 52 GLY 53 PHE 54 ILE 55 GLN
56 GLY 57 PRO 58 LEU 59 ASP 60 ASP
61 TYR 62 ALA 63 ASP 64 GLU 65 ASP
66 VAL 67 ASP 68 VAL 69 VAL 70 PHE
71 THR 72 SER
stop_
_Sequence_homology_query_date .
_Sequence_homology_query_revised_last_date .
save_
####################
# Natural source #
####################
save_natural_source
_Saveframe_category natural_source
loop_
_Mol_label
_Organism_name_common
_NCBI_taxonomy_ID
_Superkingdom
_Kingdom
_Genus
_Species
_Strain
_Gene_mnemonic
_Details
$L_Protein 'Vesicular Stomatitis Virus' 11276 Viruses . Vesiculovirus 'Vesicular Stomatitis Virus' Indiana Phosphoprotein 'Residues 35 - 106'
stop_
save_
#########################
# Experimental source #
#########################
save_experimental_source
_Saveframe_category experimental_source
loop_
_Mol_label
_Production_method
_Host_organism_name_common
_Genus
_Species
_Strain
_Vector_name
$L_Protein 'recombinant technology' . Escherichia coli . SpGFTk
stop_
save_
#####################################
# Sample contents and methodology #
#####################################
########################
# Sample description #
########################
save_sample_1
_Saveframe_category sample
_Sample_type solution
_Details 'Standard 15N (NH4Cl) and 13C (Glucose) labeling was used.'
loop_
_Mol_label
_Concentration_value
_Concentration_value_units
_Isotopic_labeling
$L_Protein 1 mM '[U-13C; U-15N]'
HEPES 20 mM 'natural abundance'
'Sodium Chloride' 150 mM 'natural abundance'
glycerol 10 % 'natural abundance'
TCEP 1 mM 'natural abundance'
'sodium azide' 0.02 % 'natural abundance'
stop_
save_
############################
# Computer software used #
############################
save_VNMRJ
_Saveframe_category software
_Name VNMRJ
_Version .
loop_
_Vendor
_Address
_Electronic_address
Varian . .
stop_
loop_
_Task
'chemical shift assignment'
stop_
_Details .
save_
#########################
# Experimental detail #
#########################
##################################
# NMR Spectrometer definitions #
##################################
save_spectrometer_1
_Saveframe_category NMR_spectrometer
_Manufacturer Bruker
_Model Avance
_Field_strength 600
_Details .
save_
#############################
# NMR applied experiments #
#############################
save_2D_1H-15N_HSQC_1
_Saveframe_category NMR_applied_experiment
_Experiment_name '2D 1H-15N HSQC'
_Sample_label $sample_1
save_
save_3D_HNCACB_2
_Saveframe_category NMR_applied_experiment
_Experiment_name '3D HNCACB'
_Sample_label $sample_1
save_
save_3D_CBCA(CO)NH_3
_Saveframe_category NMR_applied_experiment
_Experiment_name '3D CBCA(CO)NH'
_Sample_label $sample_1
save_
#######################
# Sample conditions #
#######################
save_sample_conditions_1
_Saveframe_category sample_conditions
_Details .
loop_
_Variable_type
_Variable_value
_Variable_value_error
_Variable_value_units
'ionic strength' 0.150 . M
pH 7.0 . pH
pressure 1 . atm
temperature 298 . K
stop_
save_
####################
# NMR parameters #
####################
##############################
# Assigned chemical shifts #
##############################
################################
# Chemical shift referencing #
################################
save_chemical_shift_reference_1
_Saveframe_category chemical_shift_reference
_Details .
loop_
_Mol_common_name
_Atom_type
_Atom_isotope_number
_Atom_group
_Chem_shift_units
_Chem_shift_value
_Reference_method
_Reference_type
_External_reference_sample_geometry
_External_reference_location
_External_reference_axis
_Indirect_shift_ratio
DSS C 13 'methyl protons' ppm 0 na indirect . . . 0.251449530
DSS H 1 'methyl protons' ppm 0 internal direct . . . 1.000000000
DSS N 15 'methyl protons' ppm 0 na indirect . . . 0.101329118
stop_
save_
###################################
# Assigned chemical shift lists #
###################################
###################################################################
# Chemical Shift Ambiguity Index Value Definitions #
# #
# The values other than 1 are used for those atoms with different #
# chemical shifts that cannot be assigned to stereospecific atoms #
# or to specific residues or chains. #
# #
# Index Value Definition #
# #
# 1 Unique (including isolated methyl protons, #
# geminal atoms, and geminal methyl #
# groups with identical chemical shifts) #
# (e.g. ILE HD11, HD12, HD13 protons) #
# 2 Ambiguity of geminal atoms or geminal methyl #
# proton groups (e.g. ASP HB2 and HB3 #
# protons, LEU CD1 and CD2 carbons, or #
# LEU HD11, HD12, HD13 and HD21, HD22, #
# HD23 methyl protons) #
# 3 Aromatic atoms on opposite sides of #
# symmetrical rings (e.g. TYR HE1 and HE2 #
# protons) #
# 4 Intraresidue ambiguities (e.g. LYS HG and #
# HD protons or TRP HZ2 and HZ3 protons) #
# 5 Interresidue ambiguities (LYS 12 vs. LYS 27) #
# 6 Intermolecular ambiguities (e.g. ASP 31 CA #
# in monomer 1 and ASP 31 CA in monomer 2 #
# of an asymmetrical homodimer, duplex #
# DNA assignments, or other assignments #
# that may apply to atoms in one or more #
# molecule in the molecular assembly) #
# 9 Ambiguous, specific ambiguity not defined #
# #
###################################################################
save_assigned_chem_shift_list_1
_Saveframe_category assigned_chemical_shifts
_Details .
loop_
_Experiment_label
'2D 1H-15N HSQC'
'3D HNCACB'
'3D CBCA(CO)NH'
stop_
loop_
_Sample_label
$sample_1
stop_
_Sample_conditions_label $sample_conditions_1
_Chem_shift_reference_set_label $chemical_shift_reference_1
_Mol_system_component_name 'VSV Phosphoprotein 35-106'
_Text_data_format .
_Text_data .
loop_
_Atom_shift_assign_ID
_Residue_author_seq_code
_Residue_seq_code
_Residue_label
_Atom_name
_Atom_type
_Chem_shift_value
_Chem_shift_value_error
_Chem_shift_ambiguity_code
1 4 4 GLU H H 8.249 0.020 1
2 4 4 GLU CA C 56.602 0.3 1
3 4 4 GLU CB C 30.214 0.3 1
4 4 4 GLU N N 121.896 0.3 1
5 5 5 LEU H H 8.004 0.020 1
6 5 5 LEU CA C 55.120 0.3 1
7 5 5 LEU CB C 42.412 0.3 1
8 5 5 LEU N N 122.422 0.3 1
9 6 6 PHE H H 8.168 0.020 1
10 6 6 PHE CA C 57.518 0.3 1
11 6 6 PHE CB C 39.720 0.3 1
12 6 6 PHE N N 120.672 0.3 1
13 7 7 GLN H H 8.180 0.020 1
14 7 7 GLN CA C 55.389 0.3 1
15 7 7 GLN CB C 29.810 0.3 1
16 7 7 GLN N N 122.385 0.3 1
17 8 8 GLU H H 8.428 0.020 1
18 8 8 GLU CA C 56.533 0.3 1
19 8 8 GLU CB C 30.163 0.3 1
20 8 8 GLU N N 122.784 0.3 1
21 10 10 GLY H H 8.368 0.020 1
22 10 10 GLY CA C 45.499 0.3 1
23 10 10 GLY N N 109.405 0.3 1
24 11 11 VAL H H 7.909 0.020 1
25 11 11 VAL CA C 62.318 0.3 1
26 11 11 VAL CB C 32.624 0.3 1
27 11 11 VAL N N 119.224 0.3 1
28 15 15 THR H H 8.044 0.020 1
29 15 15 THR CA C 61.911 0.3 1
30 15 15 THR CB C 69.641 0.3 1
31 15 15 THR N N 115.016 0.3 1
32 16 16 ARG H H 8.288 0.020 1
33 16 16 ARG CA C 54.041 0.3 1
34 16 16 ARG CB C 30.199 0.3 1
35 16 16 ARG N N 124.818 0.3 1
36 18 18 SER H H 8.353 0.020 1
37 18 18 SER CA C 58.382 0.3 1
38 18 18 SER CB C 63.675 0.3 1
39 18 18 SER N N 115.633 0.3 1
40 19 19 TYR H H 7.935 0.020 1
41 19 19 TYR CA C 57.592 0.3 1
42 19 19 TYR CB C 38.695 0.3 1
43 19 19 TYR N N 121.369 0.3 1
44 20 20 PHE H H 7.990 0.020 1
45 20 20 PHE CA C 57.598 0.3 1
46 20 20 PHE CB C 39.770 0.3 1
47 20 20 PHE N N 122.019 0.3 1
48 21 21 GLN H H 8.095 0.020 1
49 21 21 GLN CA C 55.284 0.3 1
50 21 21 GLN CB C 29.791 0.3 1
51 21 21 GLN N N 123.193 0.3 1
52 22 22 ALA H H 8.293 0.020 1
53 22 22 ALA CA C 52.293 0.3 1
54 22 22 ALA CB C 19.311 0.3 1
55 22 22 ALA N N 126.497 0.3 1
56 23 23 ALA H H 8.340 0.020 1
57 23 23 ALA CA C 52.312 0.3 1
58 23 23 ALA CB C 19.330 0.3 1
59 23 23 ALA N N 123.709 0.3 1
60 24 24 ASP H H 8.304 0.020 1
61 24 24 ASP CA C 54.239 0.3 1
62 24 24 ASP CB C 41.269 0.3 1
63 24 24 ASP N N 119.758 0.3 1
64 25 25 ASP H H 8.274 0.020 1
65 25 25 ASP CA C 54.294 0.3 1
66 25 25 ASP CB C 41.020 0.3 1
67 25 25 ASP N N 121.179 0.3 1
68 26 26 SER H H 8.270 0.020 1
69 26 26 SER CA C 58.853 0.3 1
70 26 26 SER CB C 63.924 0.3 1
71 26 26 SER N N 115.907 0.3 1
72 27 27 ASP H H 8.414 0.020 1
73 27 27 ASP CA C 54.349 0.3 1
74 27 27 ASP CB C 41.073 0.3 1
75 27 27 ASP N N 122.725 0.3 1
76 28 28 THR H H 8.073 0.020 1
77 28 28 THR CA C 61.997 0.3 1
78 28 28 THR CB C 69.817 0.3 1
79 28 28 THR N N 114.352 0.3 1
80 29 29 GLU H H 8.423 0.020 1
81 29 29 GLU CA C 56.373 0.3 1
82 29 29 GLU CB C 30.277 0.3 1
83 29 29 GLU N N 123.471 0.3 1
84 30 30 SER H H 8.293 0.020 1
85 30 30 SER CA C 58.184 0.3 1
86 30 30 SER CB C 63.982 0.3 1
87 30 30 SER N N 117.334 0.3 1
88 31 31 GLU H H 8.462 0.020 1
89 31 31 GLU CA C 54.396 0.3 1
90 31 31 GLU CB C 29.717 0.3 1
91 31 31 GLU N N 124.105 0.3 1
92 33 33 GLU H H 8.513 0.020 1
93 33 33 GLU CA C 56.339 0.3 1
94 33 33 GLU CB C 30.200 0.3 1
95 33 33 GLU N N 121.628 0.3 1
96 34 34 ILE H H 8.244 0.020 1
97 34 34 ILE CA C 60.838 0.3 1
98 34 34 ILE CB C 38.853 0.3 1
99 34 34 ILE N N 122.590 0.3 1
100 35 35 GLU H H 8.503 0.020 1
101 35 35 GLU CA C 56.505 0.3 1
102 35 35 GLU CB C 30.517 0.3 1
103 35 35 GLU N N 125.702 0.3 1
104 36 36 ASP H H 8.304 0.020 1
105 36 36 ASP CA C 54.245 0.3 1
106 36 36 ASP CB C 41.148 0.3 1
107 36 36 ASP N N 119.574 0.3 1
108 37 37 ASN H H 8.442 0.020 1
109 37 37 ASN CA C 53.172 0.3 1
110 37 37 ASN CB C 38.636 0.3 1
111 37 37 ASN N N 119.839 0.3 1
112 38 38 GLN H H 8.453 0.020 1
113 38 38 GLN CA C 56.449 0.3 1
114 38 38 GLN CB C 29.057 0.3 1
115 38 38 GLN N N 120.063 0.3 1
116 39 39 GLY H H 8.416 0.020 1
117 39 39 GLY CA C 45.400 0.3 1
118 39 39 GLY N N 109.273 0.3 1
119 40 40 LEU H H 7.906 0.020 1
120 40 40 LEU CA C 55.019 0.3 1
121 40 40 LEU CB C 42.432 0.3 1
122 40 40 LEU N N 121.384 0.3 1
123 41 41 TYR H H 8.215 0.020 1
124 41 41 TYR CA C 57.663 0.3 1
125 41 41 TYR CB C 38.656 0.3 1
126 41 41 TYR N N 122.152 0.3 1
127 42 42 VAL H H 7.954 0.020 1
128 42 42 VAL CA C 59.139 0.3 1
129 42 42 VAL CB C 33.204 0.3 1
130 42 42 VAL N N 126.620 0.3 1
131 44 44 ASP H H 8.330 0.020 1
132 44 44 ASP N N 121.643 0.3 1
133 46 46 GLU H H 8.414 0.020 1
134 46 46 GLU CA C 56.581 0.3 1
135 46 46 GLU CB C 29.817 0.3 1
136 46 46 GLU N N 119.438 0.3 1
137 47 47 ALA H H 7.964 0.020 1
138 47 47 ALA CA C 52.591 0.3 1
139 47 47 ALA CB C 19.288 0.3 1
140 47 47 ALA N N 124.109 0.3 1
141 48 48 GLU H H 8.247 0.020 1
142 48 48 GLU CA C 56.601 0.3 1
143 48 48 GLU CB C 30.295 0.3 1
144 48 48 GLU N N 119.814 0.3 1
145 49 49 GLN H H 8.330 0.020 1
146 49 49 GLN CA C 55.483 0.3 1
147 49 49 GLN CB C 29.282 0.3 1
148 49 49 GLN N N 121.772 0.3 1
149 50 50 VAL H H 8.182 0.020 1
150 50 50 VAL CA C 62.162 0.3 1
151 50 50 VAL CB C 32.843 0.3 1
152 50 50 VAL N N 121.729 0.3 1
153 51 51 GLU H H 8.522 0.020 1
154 51 51 GLU CA C 56.743 0.3 1
155 51 51 GLU CB C 30.199 0.3 1
156 51 51 GLU N N 124.781 0.3 1
157 52 52 GLY H H 8.421 0.020 1
158 52 52 GLY CA C 45.140 0.3 1
159 52 52 GLY N N 110.395 0.3 1
160 53 53 PHE H H 8.034 0.020 1
161 53 53 PHE CA C 57.807 0.3 1
162 53 53 PHE CB C 39.708 0.3 1
163 53 53 PHE N N 120.352 0.3 1
164 54 54 ILE H H 8.028 0.020 1
165 54 54 ILE CA C 60.567 0.3 1
166 54 54 ILE CB C 38.837 0.3 1
167 54 54 ILE N N 124.417 0.3 1
168 55 55 GLN H H 8.396 0.020 1
169 55 55 GLN CA C 55.789 0.3 1
170 55 55 GLN CB C 29.722 0.3 1
171 55 55 GLN N N 125.556 0.3 1
172 56 56 GLY H H 8.351 0.020 1
173 56 56 GLY CA C 44.425 0.3 1
174 56 56 GLY N N 111.900 0.3 1
175 58 58 LEU H H 8.633 0.020 1
176 58 58 LEU CA C 54.769 0.3 1
177 58 58 LEU CB C 42.165 0.3 1
178 58 58 LEU N N 123.086 0.3 1
179 59 59 ASP H H 8.172 0.020 1
180 59 59 ASP CA C 54.343 0.3 1
181 59 59 ASP CB C 41.245 0.3 1
182 59 59 ASP N N 120.963 0.3 1
183 60 60 ASP H H 8.161 0.020 1
184 60 60 ASP CA C 54.131 0.3 1
185 60 60 ASP CB C 41.062 0.3 1
186 60 60 ASP N N 120.229 0.3 1
187 61 61 TYR H H 8.077 0.020 1
188 61 61 TYR CA C 57.852 0.3 1
189 61 61 TYR CB C 38.602 0.3 1
190 61 61 TYR N N 120.560 0.3 1
191 62 62 ALA H H 8.061 0.020 1
192 62 62 ALA CA C 52.226 0.3 1
193 62 62 ALA CB C 19.632 0.3 1
194 62 62 ALA N N 126.073 0.3 1
195 63 63 ASP H H 8.194 0.020 1
196 63 63 ASP CA C 54.276 0.3 1
197 63 63 ASP CB C 41.184 0.3 1
198 63 63 ASP N N 120.240 0.3 1
199 64 64 GLU H H 8.317 0.020 1
200 64 64 GLU CA C 56.516 0.3 1
201 64 64 GLU CB C 30.536 0.3 1
202 64 64 GLU N N 120.946 0.3 1
203 65 65 ASP H H 8.430 0.020 1
204 65 65 ASP CA C 54.349 0.3 1
205 65 65 ASP CB C 41.073 0.3 1
206 65 65 ASP N N 121.471 0.3 1
207 66 66 VAL H H 7.944 0.020 1
208 66 66 VAL CA C 62.103 0.3 1
209 66 66 VAL CB C 32.882 0.3 1
210 66 66 VAL N N 119.497 0.3 1
211 67 67 ASP H H 8.384 0.020 1
212 67 67 ASP CA C 54.416 0.3 1
213 67 67 ASP CB C 41.131 0.3 1
214 67 67 ASP N N 123.746 0.3 1
215 68 68 VAL H H 7.949 0.020 1
216 68 68 VAL CA C 62.306 0.3 1
217 68 68 VAL CB C 32.663 0.3 1
218 68 68 VAL N N 120.675 0.3 1
219 69 69 VAL H H 8.098 0.020 1
220 69 69 VAL CA C 62.122 0.3 1
221 69 69 VAL CB C 32.805 0.3 1
222 69 69 VAL N N 124.112 0.3 1
223 70 70 PHE H H 8.417 0.020 1
224 70 70 PHE CA C 57.617 0.3 1
225 70 70 PHE CB C 39.736 0.3 1
226 70 70 PHE N N 124.872 0.3 1
227 71 71 THR H H 8.113 0.020 1
228 71 71 THR CA C 61.438 0.3 1
229 71 71 THR CB C 70.011 0.3 1
230 71 71 THR N N 116.451 0.3 1
231 72 72 SER H H 7.884 0.020 1
232 72 72 SER CA C 60.014 0.3 1
233 72 72 SER CB C 64.742 0.3 1
234 72 72 SER N N 123.350 0.3 1
stop_
save_