data_27866 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; A ubiquitin-like dimerization domain controls protein kinase D activation by trans-autophosphorylation ; _BMRB_accession_number 27866 _BMRB_flat_file_name bmr27866.str _Entry_type original _Submission_date 2019-04-02 _Accession_date 2019-04-02 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Leonard Thomas . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 124 "13C chemical shifts" 115 "15N chemical shifts" 106 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-01-10 update BMRB 'update entry citation' 2019-08-14 original author 'original release' stop_ _Original_release_date 2019-04-03 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; A ubiquitin-like dimerization domain controls protein kinase D activation by trans-autophosphorylation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31406020 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Elsner Daniel J. . 2 Siess Katharina M. . 3 Gossenreiter Thomas . . 4 Hartl Markus . . 5 Leonard Thomas A. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 294 _Journal_issue 39 _Journal_ISSN 1083-351X _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 14422 _Page_last 14441 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name homodimer _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Dkf1, chain 1' $Dkf1 'Dkf1, chain 2' $Dkf1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Dkf1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Dkf1 _Molecular_mass . _Mol_thiol_state reduced _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 153 _Mol_residue_sequence ; GAMDGSQGSTDYGDHVVLRY GGTREMVPLIRHEQMLDMLM ERARQIVQGFGNLDTRNMYL FRHEYNSPTLLYPITSASQI TSGSILEIILVDRTEAAVIP HVVEPESYMRPTFCDFCGEM LTGLMRQGVKCKNCNGNFHK RCSNAARNNCGAP ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 -1 GLY 2 0 ALA 3 1 MET 4 2 ASP 5 3 GLY 6 4 SER 7 5 GLN 8 6 GLY 9 7 SER 10 8 THR 11 9 ASP 12 10 TYR 13 11 GLY 14 12 ASP 15 13 HIS 16 14 VAL 17 15 VAL 18 16 LEU 19 17 ARG 20 18 TYR 21 19 GLY 22 20 GLY 23 21 THR 24 22 ARG 25 23 GLU 26 24 MET 27 25 VAL 28 26 PRO 29 27 LEU 30 28 ILE 31 29 ARG 32 30 HIS 33 31 GLU 34 32 GLN 35 33 MET 36 34 LEU 37 35 ASP 38 36 MET 39 37 LEU 40 38 MET 41 39 GLU 42 40 ARG 43 41 ALA 44 42 ARG 45 43 GLN 46 44 ILE 47 45 VAL 48 46 GLN 49 47 GLY 50 48 PHE 51 49 GLY 52 50 ASN 53 51 LEU 54 52 ASP 55 53 THR 56 54 ARG 57 55 ASN 58 56 MET 59 57 TYR 60 58 LEU 61 59 PHE 62 60 ARG 63 61 HIS 64 62 GLU 65 63 TYR 66 64 ASN 67 65 SER 68 66 PRO 69 67 THR 70 68 LEU 71 69 LEU 72 70 TYR 73 71 PRO 74 72 ILE 75 73 THR 76 74 SER 77 75 ALA 78 76 SER 79 77 GLN 80 78 ILE 81 79 THR 82 80 SER 83 81 GLY 84 82 SER 85 83 ILE 86 84 LEU 87 85 GLU 88 86 ILE 89 87 ILE 90 88 LEU 91 89 VAL 92 90 ASP 93 91 ARG 94 92 THR 95 93 GLU 96 94 ALA 97 95 ALA 98 96 VAL 99 97 ILE 100 98 PRO 101 99 HIS 102 100 VAL 103 101 VAL 104 102 GLU 105 103 PRO 106 104 GLU 107 105 SER 108 106 TYR 109 107 MET 110 108 ARG 111 109 PRO 112 110 THR 113 111 PHE 114 112 CYS 115 113 ASP 116 114 PHE 117 115 CYS 118 116 GLY 119 117 GLU 120 118 MET 121 119 LEU 122 120 THR 123 121 GLY 124 122 LEU 125 123 MET 126 124 ARG 127 125 GLN 128 126 GLY 129 127 VAL 130 128 LYS 131 129 CYS 132 130 LYS 133 131 ASN 134 132 CYS 135 133 ASN 136 134 GLY 137 135 ASN 138 136 PHE 139 137 HIS 140 138 LYS 141 139 ARG 142 140 CYS 143 141 SER 144 142 ASN 145 143 ALA 146 144 ALA 147 145 ARG 148 146 ASN 149 147 ASN 150 148 CYS 151 149 GLY 152 150 ALA 153 151 PRO stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Dkf1 Roundworm 6239 Eukaryota Metazoa Caenorhabditis elegans stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Dkf1 'recombinant technology' . Escherichia coli . pGST-parallel stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_Dkf1_ULD-C1a _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Dkf1 0.3 mM [U-15N] KCl 150 mM 'natural abundance' stop_ save_ save_DKf1_ULD _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Dkf1 1.2 mM '[U-13C; U-15N]' KCl 150 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_Sparky _Saveframe_category software _Name Sparky _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $DKf1_ULD save_ save_3D_HNHA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNHA' _Sample_label $DKf1_ULD save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $DKf1_ULD save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $DKf1_ULD save_ save_2D_1H-15N_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $Dkf1_ULD-C1a save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.135 . M pH 7.2 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNHA' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $DKf1_ULD stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Dkf1, chain 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 9 11 ASP CA C 53.990002 . 1 2 9 11 ASP CB C 41.200001 . 1 3 10 12 TYR H H 8.156000 . 1 4 10 12 TYR HA H 4.479000 . 1 5 10 12 TYR CA C 58.099998 . 1 6 10 12 TYR CB C 38.439999 . 1 7 10 12 TYR N N 121.199997 . 1 8 11 13 GLY H H 8.318000 . 1 9 11 13 GLY HA2 H 3.965000 . 1 10 11 13 GLY HA3 H 3.965000 . 1 11 11 13 GLY CA C 45.090000 . 1 12 11 13 GLY N N 108.900002 . 1 13 12 14 ASP H H 8.540000 . 1 14 12 14 ASP CA C 54.810001 . 1 15 12 14 ASP CB C 40.900002 . 1 16 12 14 ASP N N 124.099998 . 1 17 13 15 HIS H H 8.333000 . 1 18 13 15 HIS CA C 56.330002 . 1 19 13 15 HIS CB C 32.560001 . 1 20 13 15 HIS N N 120.900002 . 1 21 14 16 VAL H H 8.835000 . 1 22 14 16 VAL CA C 59.650002 . 1 23 14 16 VAL CB C 35.360001 . 1 24 14 16 VAL N N 113.099998 . 1 25 15 17 VAL H H 8.604000 . 1 26 15 17 VAL CA C 61.520000 . 1 27 15 17 VAL CB C 33.730000 . 1 28 15 17 VAL N N 122.800003 . 1 29 16 18 LEU H H 8.973000 . 1 30 16 18 LEU CA C 53.320000 . 1 31 16 18 LEU CB C 44.320000 . 1 32 16 18 LEU N N 126.400002 . 1 33 17 19 ARG H H 8.871000 . 1 34 17 19 ARG CA C 55.029999 . 1 35 17 19 ARG CB C 34.580002 . 1 36 17 19 ARG N N 121.699997 . 1 37 20 22 GLY CA C 45.759998 . 1 38 21 23 THR H H 7.718000 . 1 39 21 23 THR HA H 4.414000 . 1 40 21 23 THR CA C 63.400002 . 1 41 21 23 THR CB C 70.349998 . 1 42 21 23 THR N N 119.400002 . 1 43 22 24 ARG H H 8.674000 . 1 44 22 24 ARG HA H 5.387000 . 1 45 22 24 ARG CA C 54.410000 . 1 46 22 24 ARG CB C 33.560001 . 1 47 22 24 ARG N N 125.500000 . 1 48 23 25 GLU H H 9.038000 . 1 49 23 25 GLU HA H 4.675000 . 1 50 23 25 GLU CA C 55.349998 . 1 51 23 25 GLU N N 122.900002 . 1 52 24 26 MET H H 8.788000 . 1 53 24 26 MET HA H 4.902000 . 1 54 24 26 MET CA C 55.509998 . 1 55 24 26 MET CB C 33.049999 . 1 56 24 26 MET N N 125.500000 . 1 57 25 27 VAL H H 9.260000 . 1 58 25 27 VAL HA H 4.651000 . 1 59 25 27 VAL CA C 59.110001 . 1 60 25 27 VAL CB C 34.599998 . 1 61 25 27 VAL N N 126.599998 . 1 62 26 28 PRO CA C 63.040001 . 1 63 26 28 PRO CB C 32.119999 . 1 64 27 29 LEU H H 7.977000 . 1 65 27 29 LEU CA C 53.770000 . 1 66 27 29 LEU CB C 43.279999 . 1 67 27 29 LEU N N 123.400002 . 1 68 28 30 ILE H H 7.960000 . 1 69 28 30 ILE CA C 60.130001 . 1 70 28 30 ILE CB C 39.570000 . 1 71 28 30 ILE N N 125.000000 . 1 72 32 34 GLN CA C 55.770000 . 1 73 32 34 GLN CB C 28.200001 . 1 74 33 35 MET H H 7.640000 . 1 75 33 35 MET CA C 59.150002 . 1 76 33 35 MET CB C 33.860001 . 1 77 33 35 MET N N 120.000000 . 1 78 34 36 LEU H H 8.606000 . 1 79 34 36 LEU HA H 4.019000 . 1 80 34 36 LEU CA C 58.500000 . 1 81 34 36 LEU CB C 41.240002 . 1 82 34 36 LEU N N 119.199997 . 1 83 35 37 ASP H H 7.845000 . 1 84 35 37 ASP HA H 4.110000 . 1 85 35 37 ASP CA C 57.560001 . 1 86 35 37 ASP CB C 40.099998 . 1 87 35 37 ASP N N 119.199997 . 1 88 36 38 MET H H 8.124000 . 1 89 36 38 MET HA H 4.156000 . 1 90 36 38 MET CA C 59.150002 . 1 91 36 38 MET CB C 32.020000 . 1 92 36 38 MET N N 119.400002 . 1 93 37 39 LEU H H 8.204000 . 1 94 37 39 LEU HA H 4.268000 . 1 95 37 39 LEU CA C 58.049999 . 1 96 37 39 LEU N N 121.699997 . 1 97 38 40 MET H H 8.550000 . 1 98 38 40 MET HA H 4.182000 . 1 99 38 40 MET CA C 57.169998 . 1 100 38 40 MET CB C 30.549999 . 1 101 38 40 MET N N 117.500000 . 1 102 39 41 GLU H H 7.922000 . 1 103 39 41 GLU HA H 4.112000 . 1 104 39 41 GLU CA C 59.180000 . 1 105 39 41 GLU N N 119.800003 . 1 106 40 42 ARG H H 7.824000 . 1 107 40 42 ARG CA C 57.320000 . 1 108 40 42 ARG CB C 28.370001 . 1 109 40 42 ARG N N 119.199997 . 1 110 41 43 ALA H H 8.589000 . 1 111 41 43 ALA CA C 55.619999 . 1 112 41 43 ALA CB C 18.650000 . 1 113 41 43 ALA N N 121.699997 . 1 114 42 44 ARG H H 8.294000 . 1 115 42 44 ARG CA C 60.590000 . 1 116 42 44 ARG CB C 30.049999 . 1 117 42 44 ARG N N 117.400002 . 1 118 43 45 GLN H H 7.691000 . 1 119 43 45 GLN CA C 58.910000 . 1 120 43 45 GLN CB C 27.820000 . 1 121 43 45 GLN N N 117.300003 . 1 122 44 46 ILE H H 7.903000 . 1 123 44 46 ILE CA C 65.440002 . 1 124 44 46 ILE CB C 38.189999 . 1 125 44 46 ILE N N 121.400002 . 1 126 45 47 VAL H H 7.858000 . 1 127 45 47 VAL CA C 66.230003 . 1 128 45 47 VAL CB C 31.379999 . 1 129 45 47 VAL N N 118.400002 . 1 130 46 48 GLN H H 8.471000 . 1 131 46 48 GLN CA C 58.230000 . 1 132 46 48 GLN CB C 27.950001 . 1 133 46 48 GLN N N 118.699997 . 1 134 47 49 GLY H H 7.855000 . 1 135 47 49 GLY HA2 H 3.867000 . 1 136 47 49 GLY HA3 H 3.867000 . 1 137 47 49 GLY CA C 46.130001 . 1 138 47 49 GLY N N 107.500000 . 1 139 48 50 PHE H H 7.820000 . 1 140 48 50 PHE CA C 58.930000 . 1 141 48 50 PHE CB C 39.610001 . 1 142 48 50 PHE N N 118.300003 . 1 143 49 51 GLY H H 7.897000 . 1 144 49 51 GLY CA C 45.220001 . 1 145 49 51 GLY N N 107.400002 . 1 146 52 54 ASP CA C 57.770000 . 1 147 52 54 ASP CB C 46.840000 . 1 148 53 55 THR H H 7.658000 . 1 149 53 55 THR CA C 62.049999 . 1 150 53 55 THR CB C 69.720001 . 1 151 53 55 THR N N 106.699997 . 1 152 54 56 ARG H H 8.216000 . 1 153 54 56 ARG CA C 59.139999 . 1 154 54 56 ARG CB C 30.049999 . 1 155 54 56 ARG N N 123.099998 . 1 156 55 57 ASN H H 8.672000 . 1 157 55 57 ASN CA C 52.099998 . 1 158 55 57 ASN CB C 38.580002 . 1 159 55 57 ASN N N 116.800003 . 1 160 56 58 MET H H 7.121000 . 1 161 56 58 MET CA C 56.080002 . 1 162 56 58 MET CB C 35.080002 . 1 163 56 58 MET N N 119.500000 . 1 164 57 59 TYR H H 9.134000 . 1 165 57 59 TYR CA C 57.070000 . 1 166 57 59 TYR CB C 43.810001 . 1 167 57 59 TYR N N 126.300003 . 1 168 58 60 LEU H H 8.631000 . 1 169 58 60 LEU CA C 52.650002 . 1 170 58 60 LEU CB C 43.720001 . 1 171 58 60 LEU N N 117.900002 . 1 172 59 61 PHE H H 9.440000 . 1 173 59 61 PHE CA C 56.310001 . 1 174 59 61 PHE CB C 43.099998 . 1 175 59 61 PHE N N 120.099998 . 1 176 64 66 ASN CA C 52.950001 . 1 177 64 66 ASN CB C 38.169998 . 1 178 65 67 SER H H 7.831000 . 1 179 65 67 SER CA C 55.570000 . 1 180 65 67 SER CB C 64.750000 . 1 181 65 67 SER N N 115.300003 . 1 182 71 73 PRO CA C 62.180000 . 1 183 72 74 ILE H H 8.552000 . 1 184 72 74 ILE CA C 58.860001 . 1 185 72 74 ILE N N 121.599998 . 1 186 77 79 GLN CA C 56.020000 . 1 187 77 79 GLN CB C 30.100000 . 1 188 78 80 ILE H H 7.592000 . 1 189 78 80 ILE CA C 61.380001 . 1 190 78 80 ILE CB C 37.430000 . 1 191 78 80 ILE N N 119.099998 . 1 192 79 81 THR H H 7.533000 . 1 193 79 81 THR CA C 59.570000 . 1 194 79 81 THR N N 117.599998 . 1 195 80 82 SER CA C 59.810001 . 1 196 80 82 SER CB C 62.630001 . 1 197 81 83 GLY H H 9.064000 . 1 198 81 83 GLY CA C 44.709999 . 1 199 81 83 GLY N N 115.300003 . 1 200 82 84 SER H H 7.691000 . 1 201 82 84 SER CA C 60.439999 . 1 202 82 84 SER CB C 65.209999 . 1 203 82 84 SER N N 115.199997 . 1 204 83 85 ILE H H 8.343000 . 1 205 83 85 ILE CA C 60.349998 . 1 206 83 85 ILE N N 122.300003 . 1 207 84 86 LEU H H 9.232000 . 1 208 84 86 LEU CA C 52.310001 . 1 209 84 86 LEU N N 127.000000 . 1 210 85 87 GLU CA C 54.779999 . 1 211 86 88 ILE H H 9.158000 . 1 212 86 88 ILE CA C 62.040001 . 1 213 86 88 ILE N N 126.000000 . 1 214 87 89 ILE H H 8.787000 . 1 215 87 89 ILE CA C 58.930000 . 1 216 87 89 ILE CB C 40.700001 . 1 217 87 89 ILE N N 127.500000 . 1 218 88 90 LEU H H 8.844000 . 1 219 88 90 LEU CA C 53.830002 . 1 220 88 90 LEU CB C 40.570000 . 1 221 88 90 LEU N N 128.399994 . 1 222 89 91 VAL H H 8.270000 . 1 223 89 91 VAL CA C 62.060001 . 1 224 89 91 VAL N N 124.199997 . 1 225 90 92 ASP CA C 54.419998 . 1 226 90 92 ASP CB C 42.090000 . 1 227 91 93 ARG H H 8.155000 . 1 228 91 93 ARG CA C 55.630001 . 1 229 91 93 ARG CB C 30.770000 . 1 230 91 93 ARG N N 121.900002 . 1 231 92 94 THR H H 8.156000 . 1 232 92 94 THR CA C 62.450001 . 1 233 92 94 THR N N 116.500000 . 1 234 93 95 GLU H H 8.411000 . 1 235 93 95 GLU HA H 4.297000 . 1 236 93 95 GLU CA C 56.369999 . 1 237 93 95 GLU CB C 30.340000 . 1 238 93 95 GLU N N 123.800003 . 1 239 94 96 ALA H H 7.972000 . 1 240 94 96 ALA HA H 4.085000 . 1 241 94 96 ALA CA C 53.900002 . 1 242 94 96 ALA CB C 20.000000 . 1 243 94 96 ALA N N 130.699997 . 1 stop_ save_ save_assigned_chem_shift_list_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $Dkf1_ULD-C1a stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Dkf1, chain 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 10 12 TYR H H 8.174000 . 1 2 10 12 TYR N N 121.300003 . 1 3 11 13 GLY H H 8.334000 . 1 4 11 13 GLY N N 109.000000 . 1 5 12 14 ASP H H 8.524000 . 1 6 12 14 ASP N N 124.300003 . 1 7 13 15 HIS H H 8.333000 . 1 8 13 15 HIS N N 121.099998 . 1 9 14 16 VAL H H 8.850000 . 1 10 14 16 VAL N N 113.099998 . 1 11 15 17 VAL H H 8.624000 . 1 12 15 17 VAL N N 122.800003 . 1 13 16 18 LEU H H 8.977000 . 1 14 16 18 LEU N N 126.500000 . 1 15 17 19 ARG H H 8.893000 . 1 16 17 19 ARG N N 121.800003 . 1 17 21 23 THR H H 7.708000 . 1 18 21 23 THR N N 119.500000 . 1 19 22 24 ARG H H 8.679000 . 1 20 22 24 ARG N N 125.599998 . 1 21 23 25 GLU H H 9.051000 . 1 22 23 25 GLU N N 122.900002 . 1 23 24 26 MET H H 8.810000 . 1 24 24 26 MET N N 125.599998 . 1 25 25 27 VAL H H 9.284000 . 1 26 25 27 VAL N N 126.699997 . 1 27 27 29 LEU H H 7.977000 . 1 28 27 29 LEU N N 123.500000 . 1 29 28 30 ILE H H 7.971000 . 1 30 28 30 ILE N N 125.099998 . 1 31 33 35 MET H H 7.649000 . 1 32 33 35 MET N N 120.099998 . 1 33 34 36 LEU H H 8.634000 . 1 34 34 36 LEU N N 119.300003 . 1 35 35 37 ASP H H 7.863000 . 1 36 35 37 ASP N N 119.300003 . 1 37 36 38 MET H H 8.135000 . 1 38 36 38 MET N N 119.500000 . 1 39 37 39 LEU H H 8.220000 . 1 40 37 39 LEU N N 121.800003 . 1 41 38 40 MET H H 8.561000 . 1 42 38 40 MET N N 117.599998 . 1 43 39 41 GLU H H 7.940000 . 1 44 39 41 GLU N N 120.000000 . 1 45 40 42 ARG H H 7.863000 . 1 46 40 42 ARG N N 119.300003 . 1 47 41 43 ALA H H 8.593000 . 1 48 41 43 ALA N N 121.800003 . 1 49 42 44 ARG H H 8.297000 . 1 50 42 44 ARG N N 117.500000 . 1 51 43 45 GLN H H 7.702000 . 1 52 43 45 GLN N N 117.400002 . 1 53 44 46 ILE H H 7.920000 . 1 54 44 46 ILE N N 121.400002 . 1 55 45 47 VAL H H 7.848000 . 1 56 45 47 VAL N N 118.400002 . 1 57 46 48 GLN H H 8.477000 . 1 58 46 48 GLN N N 118.699997 . 1 59 47 49 GLY H H 7.895000 . 1 60 47 49 GLY N N 107.500000 . 1 61 48 50 PHE H H 7.848000 . 1 62 48 50 PHE N N 118.400002 . 1 63 49 51 GLY H H 7.895000 . 1 64 49 51 GLY N N 107.500000 . 1 65 53 55 THR H H 7.652000 . 1 66 53 55 THR N N 107.000000 . 1 67 54 56 ARG H H 8.193000 . 1 68 54 56 ARG N N 123.199997 . 1 69 55 57 ASN H H 8.670000 . 1 70 55 57 ASN N N 116.900002 . 1 71 56 58 MET H H 7.163000 . 1 72 56 58 MET N N 119.599998 . 1 73 57 59 TYR H H 9.160000 . 1 74 57 59 TYR N N 126.400002 . 1 75 58 60 LEU H H 8.663000 . 1 76 58 60 LEU N N 117.900002 . 1 77 59 61 PHE H H 9.436000 . 1 78 59 61 PHE N N 120.099998 . 1 79 65 67 SER H H 7.836000 . 1 80 65 67 SER N N 115.199997 . 1 81 72 74 ILE H H 8.528000 . 1 82 72 74 ILE N N 121.099998 . 1 83 78 80 ILE H H 7.593000 . 1 84 78 80 ILE N N 119.199997 . 1 85 79 81 THR H H 7.474000 . 1 86 79 81 THR N N 117.500000 . 1 87 81 83 GLY H H 9.074000 . 1 88 81 83 GLY N N 115.300003 . 1 89 82 84 SER H H 7.700000 . 1 90 82 84 SER N N 115.300003 . 1 91 84 86 LEU H H 9.229000 . 1 92 84 86 LEU N N 126.900002 . 1 93 86 88 ILE H H 9.166000 . 1 94 86 88 ILE N N 125.099998 . 1 95 87 89 ILE H H 8.793000 . 1 96 87 89 ILE N N 127.699997 . 1 97 88 90 LEU H H 8.859000 . 1 98 88 90 LEU N N 128.600006 . 1 99 89 91 VAL H H 8.304000 . 1 100 89 91 VAL N N 123.900002 . 1 101 91 93 ARG H H 8.143000 . 1 102 91 93 ARG N N 121.800003 . 1 stop_ save_