data_27571 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for the bHLHZip c-MYC:MAX complex. ; _BMRB_accession_number 27571 _BMRB_flat_file_name bmr27571.str _Entry_type original _Submission_date 2018-08-06 _Accession_date 2018-08-06 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bycroft Mark . . 2 Zinzalla Giovanna . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 149 "13C chemical shifts" 466 "15N chemical shifts" 149 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-07-15 update author 'update entry citation' 2019-04-05 update author 'update assignments' 2019-01-04 update BMRB 'update entry citation' 2018-09-18 original author 'original release' stop_ _Original_release_date 2018-08-06 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Crystal Structures and Nuclear Magnetic Resonance Studies of the Apo Form of the c-MYC:MAX bHLHZip Complex Reveal a Helical Basic Region in the Absence of DNA ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31260268 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sammak Susan . . 2 Hamdani Najoua . . 3 Gorrec Fabrice . . 4 Allen Mark D. . 5 Freund Stefan D. . 6 Bycroft Mark . . 7 Zinzalla Giovanna . . stop_ _Journal_abbreviation Biochemistry _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year 2019 _Details . save_ ####################################### # Cited references within the entry # ####################################### save_citation_2 _Saveframe_category citation _Citation_full . _Citation_title ; The structure of INI1/hSNF5 RPT1 and its interactions with the c-MYC:MAX heterodimer provide insights into the interplay between MYC and the SWI/SNF chromatin remodeling complex ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 30222246 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sammak Susan . . 2 Allen Mark D. . 3 Hamdani Najoua . . 4 Bycroft Mark . . 5 Zinzalla Giovanna . . stop_ _Journal_abbreviation 'FEBS J.' _Journal_name_full . _Journal_volume 285 _Journal_issue 22 _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 4165 _Page_last 4180 _Year 2018 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name c-MYC/MAX _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label c-MYC $c-MYC MAX $MAX stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details 'c-MYC/MAX bHlHzip complex' save_ ######################## # Monomeric polymers # ######################## save_c-MYC _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common c-MYC _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 105 _Mol_residue_sequence ; GSSHHHHHHSSGLVPRGSHM NVKRRTHNVLERQRRNELKR SFFALRDQIPELENNEKAPK VVILKKATAYILSVQAEEQK LISEEDLLRKRREQLKHKLE QLRNS ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 SER 4 HIS 5 HIS 6 HIS 7 HIS 8 HIS 9 HIS 10 SER 11 SER 12 GLY 13 LEU 14 VAL 15 PRO 16 ARG 17 GLY 18 SER 19 HIS 20 MET 21 ASN 22 VAL 23 LYS 24 ARG 25 ARG 26 THR 27 HIS 28 ASN 29 VAL 30 LEU 31 GLU 32 ARG 33 GLN 34 ARG 35 ARG 36 ASN 37 GLU 38 LEU 39 LYS 40 ARG 41 SER 42 PHE 43 PHE 44 ALA 45 LEU 46 ARG 47 ASP 48 GLN 49 ILE 50 PRO 51 GLU 52 LEU 53 GLU 54 ASN 55 ASN 56 GLU 57 LYS 58 ALA 59 PRO 60 LYS 61 VAL 62 VAL 63 ILE 64 LEU 65 LYS 66 LYS 67 ALA 68 THR 69 ALA 70 TYR 71 ILE 72 LEU 73 SER 74 VAL 75 GLN 76 ALA 77 GLU 78 GLU 79 GLN 80 LYS 81 LEU 82 ILE 83 SER 84 GLU 85 GLU 86 ASP 87 LEU 88 LEU 89 ARG 90 LYS 91 ARG 92 ARG 93 GLU 94 GLN 95 LEU 96 LYS 97 HIS 98 LYS 99 LEU 100 GLU 101 GLN 102 LEU 103 ARG 104 ASN 105 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value SP P01106 c-MYC . . . . . stop_ save_ save_MAX _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common MAX _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 82 _Mol_residue_sequence ; ADKRAHHNALERKRRDHIKD SFHSLRDSVPSLQGEKASRA QILDKATEYIQYMRRKNHTH QQDIDDLKRQNALLEQQVRA LE ; loop_ _Residue_seq_code _Residue_label 1 ALA 2 ASP 3 LYS 4 ARG 5 ALA 6 HIS 7 HIS 8 ASN 9 ALA 10 LEU 11 GLU 12 ARG 13 LYS 14 ARG 15 ARG 16 ASP 17 HIS 18 ILE 19 LYS 20 ASP 21 SER 22 PHE 23 HIS 24 SER 25 LEU 26 ARG 27 ASP 28 SER 29 VAL 30 PRO 31 SER 32 LEU 33 GLN 34 GLY 35 GLU 36 LYS 37 ALA 38 SER 39 ARG 40 ALA 41 GLN 42 ILE 43 LEU 44 ASP 45 LYS 46 ALA 47 THR 48 GLU 49 TYR 50 ILE 51 GLN 52 TYR 53 MET 54 ARG 55 ARG 56 LYS 57 ASN 58 HIS 59 THR 60 HIS 61 GLN 62 GLN 63 ASP 64 ILE 65 ASP 66 ASP 67 LEU 68 LYS 69 ARG 70 GLN 71 ASN 72 ALA 73 LEU 74 LEU 75 GLU 76 GLN 77 GLN 78 VAL 79 ARG 80 ALA 81 LEU 82 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value SP P61244 MAX . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $c-MYC Human 9606 Eukaryota Metazoa Homo sapiens $MAX Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $c-MYC 'recombinant technology' . Escherichia coli . pET24a $MAX 'recombinant technology' . Escherichia coli . pET24a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $c-MYC 250 uM 200 300 '[U-100% 13C; U-100% 15N; U-80% 2H]' $MAX 250 uM 200 300 '[U-100% 13C; U-100% 15N; U-80% 2H]' 'sodium phosphate' 20 mM . . 'natural abundance' 'sodium chloride' 150 mM . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model ARX _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCO_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 150 . mM pH 7.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D 1H-15N NOESY' '3D CBCA(CO)NH' '3D HNCACB' '3D HNCO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name c-MYC _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 13 LEU H H 7.95 . . 2 14 13 LEU C C 177.36 . . 3 14 13 LEU CA C 54.94 . . 4 14 13 LEU CB C 41.58 . . 5 14 13 LEU N N 121.641 . . 6 15 14 VAL H H 7.99 . . 7 15 14 VAL CA C 59.58 . . 8 15 14 VAL CB C 32.00 . . 9 15 14 VAL N N 122.67 . . 10 16 15 PRO C C 177.19 . . 11 16 15 PRO CA C 62.92 . . 12 16 15 PRO CB C 31.45 . . 13 17 16 ARG H H 8.39 . . 14 17 16 ARG C C 177.27 . . 15 17 16 ARG CA C 56.18 . . 16 17 16 ARG CB C 30.09 . . 17 17 16 ARG N N 122.00 . . 18 21 21 ASN C C 175.404 . . 19 21 21 ASN CA C 53.225 . . 20 21 21 ASN CB C 38.366 . . 21 22 22 VAL H H 7.925 . . 22 22 22 VAL C C 176.43 . . 23 22 22 VAL CA C 62.37 . . 24 22 22 VAL CB C 31.96 . . 25 22 22 VAL N N 120.495 . . 26 23 23 LYS H H 8.255 . . 27 23 23 LYS C C 177.09 . . 28 23 23 LYS CA C 56.3 . . 29 23 23 LYS CB C 32.08 . . 30 23 23 LYS N N 124.795 . . 31 24 24 ARG H H 8.321 . . 32 24 24 ARG CA C 56.15 . . 33 24 24 ARG CB C 29.69 . . 34 24 24 ARG N N 121.722 . . 35 26 26 THR C C 175.56 . . 36 26 26 THR CB C 68.97 . . 37 27 27 HIS H H 8.205 . . 38 27 27 HIS C C 175.81 . . 39 27 27 HIS CA C 55.33 . . 40 27 27 HIS CB C 28.98 . . 41 27 27 HIS N N 122.535 . . 42 28 28 ASN H H 8.107 . . 43 28 28 ASN C C 174.9 . . 44 28 28 ASN CA C 53.74 . . 45 28 28 ASN CB C 37.63 . . 46 28 28 ASN N N 123.795 . . 47 29 29 VAL H H 8.078 . . 48 29 29 VAL C C 177.28 . . 49 29 29 VAL CA C 64 . . 50 29 29 VAL CB C 31.62 . . 51 29 29 VAL N N 121.195 . . 52 30 30 LEU H H 8.071 . . 53 30 30 LEU C C 178.76 . . 54 30 30 LEU CA C 56.29 . . 55 30 30 LEU CB C 40.99 . . 56 30 30 LEU N N 123.485 . . 57 31 31 GLU H H 8.155 . . 58 31 31 GLU C C 178.17 . . 59 31 31 GLU CA C 57.88 . . 60 31 31 GLU CB C 29.1 . . 61 31 31 GLU N N 120.835 . . 62 32 32 ARG H H 8.05 . . 63 32 32 ARG C C 177.863 . . 64 32 32 ARG CA C 57.89 . . 65 32 32 ARG CB C 29.41 . . 66 32 32 ARG N N 120.445 . . 67 33 33 GLN H H 8.114 . . 68 33 33 GLN C C 177.93 . . 69 33 33 GLN CA C 57.5 . . 70 33 33 GLN CB C 29.14 . . 71 33 33 GLN N N 119.955 . . 72 34 34 ARG H H 8.23 . . 73 34 34 ARG CA C 57.97 . . 74 34 34 ARG CB C 29.64 . . 75 34 34 ARG N N 120.89 . . 76 38 38 LEU C C 178.48 . . 77 38 38 LEU CA C 57.04 . . 78 38 38 LEU CB C 40.84 . . 79 39 39 LYS H H 7.775 . . 80 39 39 LYS C C 178.101 . . 81 39 39 LYS CA C 60.164 . . 82 39 39 LYS CB C 31.495 . . 83 39 39 LYS N N 119.715 . . 84 40 40 ARG H H 7.866 . . 85 40 40 ARG C C 180.153 . . 86 40 40 ARG CA C 59.374 . . 87 40 40 ARG CB C 28.738 . . 88 40 40 ARG N N 117.282 . . 89 41 41 SER H H 8.171 . . 90 41 41 SER C C 177.473 . . 91 41 41 SER CA C 63.315 . . 92 41 41 SER CB C 62.538 . . 93 41 41 SER N N 118.019 . . 94 42 42 PHE H H 8.605 . . 95 42 42 PHE C C 178.9 . . 96 42 42 PHE CA C 63.487 . . 97 42 42 PHE CB C 38.81 . . 98 42 42 PHE N N 123.225 . . 99 43 43 PHE H H 8.228 . . 100 43 43 PHE C C 177.368 . . 101 43 43 PHE CA C 58.223 . . 102 43 43 PHE CB C 36.615 . . 103 43 43 PHE N N 118.585 . . 104 44 44 ALA H H 8.04 . . 105 44 44 ALA C C 180.009 . . 106 44 44 ALA CA C 54.672 . . 107 44 44 ALA CB C 17.612 . . 108 44 44 ALA N N 121.377 . . 109 45 45 LEU H H 7.294 . . 110 45 45 LEU C C 178.805 . . 111 45 45 LEU CA C 57.689 . . 112 45 45 LEU CB C 40.061 . . 113 45 45 LEU N N 119.082 . . 114 46 46 ARG H H 8.204 . . 115 46 46 ARG C C 177.745 . . 116 46 46 ARG CA C 59.441 . . 117 46 46 ARG CB C 29.188 . . 118 46 46 ARG N N 118.397 . . 119 47 47 ASP H H 7.605 . . 120 47 47 ASP C C 177.279 . . 121 47 47 ASP CA C 55.729 . . 122 47 47 ASP CB C 39.343 . . 123 47 47 ASP N N 113.031 . . 124 48 48 GLN H H 7.824 . . 125 48 48 GLN C C 174.705 . . 126 48 48 GLN CA C 54.443 . . 127 48 48 GLN CB C 28.035 . . 128 48 48 GLN N N 117.248 . . 129 49 49 ILE H H 7.54 . . 130 49 49 ILE C C 174.966 . . 131 49 49 ILE CA C 56.747 . . 132 49 49 ILE CB C 36.966 . . 133 49 49 ILE N N 120.838 . . 134 50 50 PRO C C 179.51 . . 135 50 50 PRO CA C 65.95 . . 136 50 50 PRO CB C 31.33 . . 137 51 51 GLU H H 8.73 . . 138 51 51 GLU C C 177.124 . . 139 51 51 GLU CA C 58.346 . . 140 51 51 GLU CB C 29.287 . . 141 51 51 GLU N N 113.777 . . 142 52 52 LEU H H 7.837 . . 143 52 52 LEU C C 176.292 . . 144 52 52 LEU CA C 53.549 . . 145 52 52 LEU CB C 43.071 . . 146 52 52 LEU N N 116.825 . . 147 53 53 GLU H H 7.335 . . 148 53 53 GLU C C 177.568 . . 149 53 53 GLU CA C 58.118 . . 150 53 53 GLU CB C 28.682 . . 151 53 53 GLU N N 121.4 . . 152 54 54 ASN H H 8.859 . . 153 54 54 ASN C C 174.273 . . 154 54 54 ASN CA C 54.453 . . 155 54 54 ASN CB C 37.923 . . 156 54 54 ASN N N 117.458 . . 157 55 55 ASN H H 7.895 . . 158 55 55 ASN C C 175.682 . . 159 55 55 ASN CA C 52.054 . . 160 55 55 ASN CB C 37.051 . . 161 55 55 ASN N N 117.201 . . 162 56 56 GLU H H 8.548 . . 163 56 56 GLU C C 177.218 . . 164 56 56 GLU CA C 57.87 . . 165 56 56 GLU CB C 28.822 . . 166 56 56 GLU N N 124.332 . . 167 57 57 LYS H H 7.931 . . 168 57 57 LYS C C 176.802 . . 169 57 57 LYS CA C 55.053 . . 170 57 57 LYS CB C 31.354 . . 171 57 57 LYS N N 117.109 . . 172 58 58 ALA H H 7.001 . . 173 58 58 ALA C C 175.227 . . 174 58 58 ALA CA C 50.303 . . 175 58 58 ALA CB C 17.204 . . 176 58 58 ALA N N 124.783 . . 177 60 60 LYS C C 178.846 . . 178 60 60 LYS CA C 60.44 . . 179 60 60 LYS CB C 31.664 . . 180 61 61 VAL H H 8.562 . . 181 61 61 VAL C C 176.802 . . 182 61 61 VAL CA C 65.657 . . 183 61 61 VAL CB C 30.721 . . 184 61 61 VAL N N 114.732 . . 185 62 62 VAL H H 6.53 . . 186 62 62 VAL C C 177.879 . . 187 62 62 VAL CA C 65.629 . . 188 62 62 VAL CB C 30.974 . . 189 62 62 VAL N N 120.89 . . 190 63 63 ILE H H 7.747 . . 191 63 63 ILE C C 177.673 . . 192 63 63 ILE CA C 66.133 . . 193 63 63 ILE CB C 37.318 . . 194 63 63 ILE N N 119.889 . . 195 64 64 LEU H H 7.811 . . 196 64 64 LEU C C 180.214 . . 197 64 64 LEU CA C 58.232 . . 198 64 64 LEU CB C 41.791 . . 199 64 64 LEU N N 115.26 . . 200 65 65 LYS H H 8.34 . . 201 65 65 LYS C C 181.196 . . 202 65 65 LYS CA C 60.145 . . 203 65 65 LYS CB C 32.029 . . 204 65 65 LYS N N 120.379 . . 205 66 66 LYS H H 9.091 . . 206 66 66 LYS C C 180.258 . . 207 66 66 LYS CA C 58.384 . . 208 66 66 LYS CB C 30.848 . . 209 66 66 LYS N N 119.548 . . 210 67 67 ALA H H 8.685 . . 211 67 67 ALA C C 178.849 . . 212 67 67 ALA CA C 55.586 . . 213 67 67 ALA CB C 18.569 . . 214 67 67 ALA N N 123.578 . . 215 68 68 THR H H 7.747 . . 216 68 68 THR C C 182.117 . . 217 68 68 THR CA C 67.19 . . 218 68 68 THR CB C 68.234 . . 219 68 68 THR N N 115.222 . . 220 69 69 ALA H H 7.589 . . 221 69 69 ALA C C 177.191 . . 222 69 69 ALA CA C 54.805 . . 223 69 69 ALA CB C 17.668 . . 224 69 69 ALA N N 120.859 . . 225 70 70 TYR H H 8.526 . . 226 70 70 TYR C C 178.467 . . 227 70 70 TYR CA C 61.25 . . 228 70 70 TYR CB C 38.415 . . 229 70 70 TYR N N 121.12 . . 230 71 71 ILE H H 8.574 . . 231 71 71 ILE C C 178.134 . . 232 71 71 ILE CA C 66.371 . . 233 71 71 ILE CB C 36.84 . . 234 71 71 ILE N N 121.984 . . 235 72 72 LEU H H 7.929 . . 236 72 72 LEU C C 181.44 . . 237 72 72 LEU CA C 58.032 . . 238 72 72 LEU CB C 40.223 . . 239 72 72 LEU N N 118.956 . . 240 73 73 SER H H 8.07 . . 241 73 73 SER C C 177.997 . . 242 73 73 SER CA C 61.745 . . 243 73 73 SER CB C 62.524 . . 244 73 73 SER N N 117.001 . . 245 74 74 VAL H H 8.598 . . 246 74 74 VAL C C 179.554 . . 247 74 74 VAL CA C 65.105 . . 248 74 74 VAL CB C 30.721 . . 249 74 74 VAL N N 121.074 . . 250 75 75 GLN H H 8.072 . . 251 75 75 GLN C C 179.571 . . 252 75 75 GLN CA C 58.984 . . 253 75 75 GLN CB C 27.5 . . 254 75 75 GLN N N 122.551 . . 255 76 76 ALA H H 7.915 . . 256 76 76 ALA C C 181.429 . . 257 76 76 ALA CA C 54.843 . . 258 76 76 ALA CB C 17.007 . . 259 76 76 ALA N N 123.85 . . 260 77 77 GLU H H 8.29 . . 261 77 77 GLU C C 178.213 . . 262 77 77 GLU CA C 58.927 . . 263 77 77 GLU CB C 28.93 . . 264 77 77 GLU N N 121.225 . . 265 78 78 GLU H H 8.011 . . 266 78 78 GLU C C 178.522 . . 267 78 78 GLU CA C 59.841 . . 268 78 78 GLU CB C 28.436 . . 269 78 78 GLU N N 120.281 . . 270 79 79 GLN H H 7.465 . . 271 79 79 GLN C C 179.288 . . 272 79 79 GLN CA C 58.499 . . 273 79 79 GLN CB C 27.5 . . 274 79 79 GLN N N 114.896 . . 275 80 80 LYS H H 7.65 . . 276 80 80 LYS C C 179.892 . . 277 80 80 LYS CA C 59.289 . . 278 80 80 LYS CB C 31.481 . . 279 80 80 LYS N N 121.081 . . 280 81 81 LEU H H 8.338 . . 281 81 81 LEU C C 179.338 . . 282 81 81 LEU CA C 57.651 . . 283 81 81 LEU CB C 39.927 . . 284 81 81 LEU N N 122.067 . . 285 82 82 ILE H H 8.5 . . 286 82 82 ILE C C 179.299 . . 287 82 82 ILE CA C 65.486 . . 288 82 82 ILE CB C 37.613 . . 289 82 82 ILE N N 120.183 . . 290 83 83 SER H H 7.598 . . 291 83 83 SER C C 176.986 . . 292 83 83 SER CA C 61.564 . . 293 83 83 SER CB C 62.58 . . 294 83 83 SER N N 114.564 . . 295 84 84 GLU H H 7.999 . . 296 84 84 GLU C C 178.94 . . 297 84 84 GLU CA C 59.441 . . 298 84 84 GLU CB C 28.977 . . 299 84 84 GLU N N 123.475 . . 300 85 85 GLU H H 8.642 . . 301 85 85 GLU C C 178.3 . . 302 85 85 GLU CA C 60.231 . . 303 85 85 GLU CB C 28.949 . . 304 85 85 GLU N N 120.292 . . 305 86 86 ASP H H 8.099 . . 306 86 86 ASP C C 179.11 . . 307 86 86 ASP CA C 57.67 . . 308 86 86 ASP CB C 41.059 . . 309 86 86 ASP N N 118.147 . . 310 87 87 LEU H H 7.689 . . 311 87 87 LEU C C 181.107 . . 312 87 87 LEU CA C 58.137 . . 313 87 87 LEU CB C 41.003 . . 314 87 87 LEU N N 119.584 . . 315 88 88 LEU H H 8.259 . . 316 88 88 LEU C C 180.475 . . 317 88 88 LEU CA C 57.67 . . 318 88 88 LEU CB C 42.53 . . 319 88 88 LEU N N 121.176 . . 320 89 89 ARG H H 8.79 . . 321 89 89 ARG C C 179.865 . . 322 89 89 ARG CA C 60.25 . . 323 89 89 ARG CB C 29.202 . . 324 89 89 ARG N N 121.798 . . 325 90 90 LYS H H 8.097 . . 326 90 90 LYS C C 179.881 . . 327 90 90 LYS CA C 59.47 . . 328 90 90 LYS CB C 31.5 . . 329 90 90 LYS N N 121.572 . . 330 91 91 ARG H H 8.01 . . 331 91 91 ARG C C 177.757 . . 332 91 91 ARG CA C 58.242 . . 333 91 91 ARG CB C 28.626 . . 334 91 91 ARG N N 121.362 . . 335 92 92 ARG H H 8.228 . . 336 92 92 ARG C C 178.772 . . 337 92 92 ARG CA C 60.184 . . 338 92 92 ARG CB C 29.202 . . 339 92 92 ARG N N 121.523 . . 340 93 93 GLU H H 7.707 . . 341 93 93 GLU C C 179.709 . . 342 93 93 GLU CA C 58.794 . . 343 93 93 GLU CB C 28.752 . . 344 93 93 GLU N N 116.762 . . 345 94 94 GLN H H 7.876 . . 346 94 94 GLN C C 180.053 . . 347 94 94 GLN CA C 58.794 . . 348 94 94 GLN CB C 27.95 . . 349 94 94 GLN N N 119.717 . . 350 95 95 LEU H H 8.544 . . 351 95 95 LEU C C 179.021 . . 352 95 95 LEU CA C 57.889 . . 353 95 95 LEU CB C 42.663 . . 354 95 95 LEU N N 122.005 . . 355 96 96 LYS H H 8.57 . . 356 96 96 LYS C C 179.909 . . 357 96 96 LYS CA C 60.041 . . 358 96 96 LYS CB C 31.354 . . 359 96 96 LYS N N 118.649 . . 360 97 97 HIS H H 8.032 . . 361 97 97 HIS C C 177.962 . . 362 97 97 HIS CA C 59.117 . . 363 97 97 HIS CB C 29.23 . . 364 97 97 HIS N N 118.508 . . 365 98 98 LYS H H 7.935 . . 366 98 98 LYS C C 178.91 . . 367 98 98 LYS CA C 58.75 . . 368 98 98 LYS CB C 31.37 . . 369 98 98 LYS N N 120.515 . . 370 99 99 LEU H H 8.104 . . 371 99 99 LEU C C 178.949 . . 372 99 99 LEU CA C 57.709 . . 373 99 99 LEU CB C 41.045 . . 374 99 99 LEU N N 119.023 . . 375 100 100 GLU H H 7.787 . . 376 100 100 GLU C C 179.06 . . 377 100 100 GLU CA C 58.603 . . 378 100 100 GLU CB C 28.794 . . 379 100 100 GLU N N 118.054 . . 380 101 101 GLN H H 7.775 . . 381 101 101 GLN C C 178.7 . . 382 101 101 GLN CA C 57.651 . . 383 101 101 GLN CB C 28.161 . . 384 101 101 GLN N N 117.724 . . 385 102 102 LEU H H 7.944 . . 386 102 102 LEU C C 179.055 . . 387 102 102 LEU CA C 56.404 . . 388 102 102 LEU CB C 41.777 . . 389 102 102 LEU N N 119.733 . . 390 103 103 ARG H H 7.919 . . 391 103 103 ARG C C 176.78 . . 392 103 103 ARG CA C 57.175 . . 393 103 103 ARG CB C 29.962 . . 394 103 103 ARG N N 118.843 . . 395 104 104 ASN H H 7.862 . . 396 104 104 ASN C C 174.545 . . 397 104 104 ASN CA C 53.168 . . 398 104 104 ASN CB C 38.936 . . 399 104 104 ASN N N 118.607 . . 400 105 105 SER H H 7.646 . . 401 105 105 SER CA C 60.203 . . 402 105 105 SER CB C 64.844 . . 403 105 105 SER N N 121.36 . . stop_ save_ save_assigned_chem_shift_list_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D 1H-15N NOESY' '3D CBCA(CO)NH' '3D HNCACB' '3D HNCO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name MAX _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 ASP H H 7.955 . . 2 2 2 ASP CA C 54.93 . . 3 2 2 ASP CB C 41.16 . . 4 2 2 ASP N N 120.82 . . 5 3 3 LYS H H 8.13 . . 6 3 3 LYS C C 177.107 . . 7 3 3 LYS CA C 56.899 . . 8 3 3 LYS CB C 31.973 . . 9 3 3 LYS N N 121.78 . . 10 4 4 ARG H H 8.314 . . 11 4 4 ARG C C 176.59 . . 12 4 4 ARG CA C 56.16 . . 13 4 4 ARG CB C 29.75 . . 14 4 4 ARG N N 121.819 . . 15 5 5 ALA H H 8.139 . . 16 5 5 ALA C C 178.029 . . 17 5 5 ALA CA C 52.56 . . 18 5 5 ALA CB C 18.48 . . 19 5 5 ALA N N 124.475 . . 20 8 8 ASN C C 175.78 . . 21 8 8 ASN CA C 54.13 . . 22 8 8 ASN CB C 38.36 . . 23 9 9 ALA H H 8.225 . . 24 9 9 ALA C C 178.8 . . 25 9 9 ALA CA C 53.491 . . 26 9 9 ALA CB C 18.31 . . 27 9 9 ALA N N 124.26 . . 28 10 10 LEU H H 7.995 . . 29 10 10 LEU C C 178.52 . . 30 10 10 LEU CA C 55.976 . . 31 10 10 LEU CB C 41.09 . . 32 10 10 LEU N N 119.985 . . 33 11 11 GLU H H 8.055 . . 34 11 11 GLU C C 177.46 . . 35 11 11 GLU CA C 57.11 . . 36 11 11 GLU CB C 29.3 . . 37 11 11 GLU N N 120.975 . . 38 12 12 ARG H H 8.09 . . 39 12 12 ARG C C 178.21 . . 40 12 12 ARG CA C 57.00 . . 41 12 12 ARG CB C 29.75 . . 42 12 12 ARG N N 121.48 . . 43 13 13 LYS H H 8.05 . . 44 13 13 LYS C C 177.546 . . 45 13 13 LYS CA C 56.91 . . 46 13 13 LYS CB C 31.90 . . 47 13 13 LYS N N 121.26 . . 48 14 14 ARG H H 8.144 . . 49 14 14 ARG C C 177.33 . . 50 14 14 ARG CA C 56.90 . . 51 14 14 ARG CB C 29.88 . . 52 14 14 ARG N N 121.75 . . 53 18 18 ILE C C 177.38 . . 54 18 18 ILE CA C 64.91 . . 55 18 18 ILE CB C 36.68 . . 56 19 19 LYS H H 8.06 . . 57 19 19 LYS C C 178.837 . . 58 19 19 LYS CA C 59.84 . . 59 19 19 LYS CB C 31.48 . . 60 19 19 LYS N N 123.38 . . 61 20 20 ASP H H 8.297 . . 62 20 20 ASP C C 179.50 . . 63 20 20 ASP CA C 57.023 . . 64 20 20 ASP CB C 40.005 . . 65 20 20 ASP N N 118.503 . . 66 21 21 SER H H 8.15 . . 67 21 21 SER C C 177.64 . . 68 21 21 SER CA C 62.26 . . 69 21 21 SER CB C 62.79 . . 70 21 21 SER N N 114.55 . . 71 22 22 PHE H H 8.645 . . 72 22 22 PHE C C 178.777 . . 73 22 22 PHE CA C 63.687 . . 74 22 22 PHE CB C 38.654 . . 75 22 22 PHE N N 124.1 . . 76 23 23 HIS H H 8.182 . . 77 23 23 HIS C C 177.451 . . 78 23 23 HIS CA C 58.775 . . 79 23 23 HIS CB C 29.16 . . 80 23 23 HIS N N 119.234 . . 81 24 24 SER H H 8.148 . . 82 24 24 SER C C 176.613 . . 83 24 24 SER CA C 61.821 . . 84 24 24 SER CB C 62.538 . . 85 24 24 SER N N 114.556 . . 86 25 25 LEU H H 7.482 . . 87 25 25 LEU C C 179.099 . . 88 25 25 LEU CA C 57.842 . . 89 25 25 LEU CB C 40.201 . . 90 25 25 LEU N N 122.969 . . 91 26 26 ARG H H 8.398 . . 92 26 26 ARG C C 177.851 . . 93 26 26 ARG CA C 59.26 . . 94 26 26 ARG CB C 28.935 . . 95 26 26 ARG N N 119.937 . . 96 27 27 ASP H H 7.901 . . 97 27 27 ASP C C 177.107 . . 98 27 27 ASP CA C 55.871 . . 99 27 27 ASP CB C 39.695 . . 100 27 27 ASP N N 114.165 . . 101 28 28 SER H H 7.774 . . 102 28 28 SER C C 172.995 . . 103 28 28 SER CA C 59.66 . . 104 28 28 SER CB C 63.916 . . 105 28 28 SER N N 115.503 . . 106 29 29 VAL H H 7.099 . . 107 29 29 VAL C C 175.549 . . 108 29 29 VAL CA C 59.346 . . 109 29 29 VAL CB C 31.917 . . 110 29 29 VAL N N 122.909 . . 111 31 31 SER C C 175.26 . . 112 31 31 SER CA C 59.38 . . 113 31 31 SER CB C 68.28 . . 114 32 32 LEU H H 7.575 . . 115 32 32 LEU C C 177.2 . . 116 32 32 LEU CA C 54.09 . . 117 32 32 LEU CB C 42.48 . . 118 32 32 LEU N N 120.585 . . 119 33 33 GLN H H 7.335 . . 120 33 33 GLN C C 177.64 . . 121 33 33 GLN CA C 57.36 . . 122 33 33 GLN CB C 27.57 . . 123 33 33 GLN N N 120.015 . . 124 34 34 GLY H H 8.695 . . 125 34 34 GLY C C 174.242 . . 126 34 34 GLY CA C 45.46 . . 127 34 34 GLY N N 112.505 . . 128 35 35 GLU H H 7.767 . . 129 35 35 GLU C C 176.808 . . 130 35 35 GLU CA C 55.053 . . 131 35 35 GLU CB C 30.64 . . 132 35 35 GLU N N 119.624 . . 133 36 36 LYS H H 8.515 . . 134 36 36 LYS C C 176.009 . . 135 36 36 LYS CA C 55.643 . . 136 36 36 LYS CB C 30.623 . . 137 36 36 LYS N N 123.651 . . 138 37 37 ALA H H 7.688 . . 139 37 37 ALA C C 176.985 . . 140 37 37 ALA CA C 51.093 . . 141 37 37 ALA CB C 21.058 . . 142 37 37 ALA N N 125.4 . . 143 38 38 SER H H 8.567 . . 144 38 38 SER C C 174.511 . . 145 38 38 SER CA C 56.623 . . 146 38 38 SER CB C 64.591 . . 147 38 38 SER N N 118.481 . . 148 39 39 ARG H H 8.595 . . 149 39 39 ARG C C 177.968 . . 150 39 39 ARG CA C 60.602 . . 151 39 39 ARG CB C 29.251 . . 152 39 39 ARG N N 119.949 . . 153 40 40 ALA H H 8.241 . . 154 40 40 ALA C C 178.994 . . 155 40 40 ALA CA C 55.405 . . 156 40 40 ALA CB C 17.528 . . 157 40 40 ALA N N 119.038 . . 158 41 41 GLN H H 7.32 . . 159 41 41 GLN C C 179.077 . . 160 41 41 GLN CA C 58.499 . . 161 41 41 GLN CB C 28.415 . . 162 41 41 GLN N N 115.856 . . 163 42 42 ILE H H 8.268 . . 164 42 42 ILE C C 177.546 . . 165 42 42 ILE CA C 65.41 . . 166 42 42 ILE CB C 37.388 . . 167 42 42 ILE N N 119.596 . . 168 43 43 LEU H H 7.679 . . 169 43 43 LEU C C 179.437 . . 170 43 43 LEU CA C 57.975 . . 171 43 43 LEU CB C 41.088 . . 172 43 43 LEU N N 116.441 . . 173 44 44 ASP H H 8.584 . . 174 44 44 ASP C C 180.22 . . 175 44 44 ASP CA C 57.366 . . 176 44 44 ASP CB C 39.4 . . 177 44 44 ASP N N 119.823 . . 178 45 45 LYS H H 8.768 . . 179 45 45 LYS C C 180.514 . . 180 45 45 LYS CA C 57.899 . . 181 45 45 LYS CB C 30.173 . . 182 45 45 LYS N N 119.864 . . 183 46 46 ALA H H 8.457 . . 184 46 46 ALA C C 178.844 . . 185 46 46 ALA CA C 55.928 . . 186 46 46 ALA CB C 17.781 . . 187 46 46 ALA N N 124.593 . . 188 47 47 THR H H 8.4 . . 189 47 47 THR C C 176.561 . . 190 47 47 THR CA C 67.75 . . 191 47 47 THR N N 117.614 . . 192 48 48 GLU H H 7.826 . . 193 48 48 GLU C C 179.16 . . 194 48 48 GLU CA C 59.003 . . 195 48 48 GLU CB C 29.92 . . 196 48 48 GLU N N 119.887 . . 197 49 49 TYR H H 8.422 . . 198 49 49 TYR C C 177.684 . . 199 49 49 TYR CA C 61.107 . . 200 49 49 TYR CB C 38.542 . . 201 49 49 TYR N N 121.717 . . 202 50 50 ILE H H 8.592 . . 203 50 50 ILE C C 178.045 . . 204 50 50 ILE CA C 66.952 . . 205 50 50 ILE CB C 37.494 . . 206 50 50 ILE N N 119.976 . . 207 51 51 GLN H H 7.837 . . 208 51 51 GLN C C 179.193 . . 209 51 51 GLN CA C 59.641 . . 210 51 51 GLN CB C 28.499 . . 211 51 51 GLN N N 117.536 . . 212 52 52 TYR H H 8.229 . . 213 52 52 TYR C C 177.951 . . 214 52 52 TYR CA C 60.526 . . 215 52 52 TYR CB C 37.613 . . 216 52 52 TYR N N 121.228 . . 217 53 53 MET H H 8.641 . . 218 53 53 MET C C 179.243 . . 219 53 53 MET CA C 55.338 . . 220 53 53 MET CB C 29.849 . . 221 53 53 MET N N 119.734 . . 222 54 54 ARG H H 8.617 . . 223 54 54 ARG C C 180.464 . . 224 54 54 ARG CA C 59.803 . . 225 54 54 ARG CB C 29.385 . . 226 54 54 ARG N N 119.784 . . 227 55 55 ARG H H 7.427 . . 228 55 55 ARG C C 179.071 . . 229 55 55 ARG CA C 59.003 . . 230 55 55 ARG CB C 29.174 . . 231 55 55 ARG N N 119.262 . . 232 56 56 LYS H H 8.441 . . 233 56 56 LYS C C 179.332 . . 234 56 56 LYS CA C 59.489 . . 235 56 56 LYS CB C 31.959 . . 236 56 56 LYS N N 123.456 . . 237 57 57 ASN H H 8.742 . . 238 57 57 ASN C C 178.888 . . 239 57 57 ASN CA C 55.624 . . 240 57 57 ASN CB C 36.122 . . 241 57 57 ASN N N 119.017 . . 242 58 58 HIS H H 7.82 . . 243 58 58 HIS C C 178.3 . . 244 58 58 HIS CA C 59.907 . . 245 58 58 HIS CB C 29.427 . . 246 58 58 HIS N N 121.873 . . 247 59 59 THR H H 8.178 . . 248 59 59 THR C C 176.552 . . 249 59 59 THR CA C 66.647 . . 250 59 59 THR CB C 68.02 . . 251 59 59 THR N N 117.623 . . 252 60 60 HIS H H 8.413 . . 253 60 60 HIS C C 177.923 . . 254 60 60 HIS CA C 58.613 . . 255 60 60 HIS CB C 29.98 . . 256 60 60 HIS N N 119.644 . . 257 61 61 GLN H H 8.163 . . 258 61 61 GLN C C 178.173 . . 259 61 61 GLN CA C 58.603 . . 260 61 61 GLN CB C 27.852 . . 261 61 61 GLN N N 117.562 . . 262 62 62 GLN H H 7.876 . . 263 62 62 GLN C C 179.265 . . 264 62 62 GLN CA C 58.651 . . 265 62 62 GLN CB C 27.852 . . 266 62 62 GLN N N 119.178 . . 267 63 63 ASP H H 8.302 . . 268 63 63 ASP C C 179.981 . . 269 63 63 ASP CA C 57.575 . . 270 63 63 ASP CB C 39.076 . . 271 63 63 ASP N N 122.229 . . 272 64 64 ILE H H 8.618 . . 273 64 64 ILE C C 177.59 . . 274 64 64 ILE CA C 65.762 . . 275 64 64 ILE CB C 37.6 . . 276 64 64 ILE N N 123.322 . . 277 65 65 ASP H H 8.426 . . 278 65 65 ASP C C 179.859 . . 279 65 65 ASP CA C 57.785 . . 280 65 65 ASP CB C 39.54 . . 281 65 65 ASP N N 121.206 . . 282 66 66 ASP H H 8.034 . . 283 66 66 ASP C C 178.971 . . 284 66 66 ASP CA C 57.556 . . 285 66 66 ASP CB C 41.07 . . 286 66 66 ASP N N 120.571 . . 287 67 67 LEU H H 8.277 . . 288 67 67 LEU C C 179.565 . . 289 67 67 LEU CA C 58.299 . . 290 67 67 LEU CB C 42.48 . . 291 67 67 LEU N N 122.043 . . 292 68 68 LYS H H 8.7 . . 293 68 68 LYS C C 180.778 . . 294 68 68 LYS CA C 60.64 . . 295 68 68 LYS CB C 31.818 . . 296 68 68 LYS N N 118.687 . . 297 69 69 ARG H H 7.703 . . 298 69 69 ARG C C 179.681 . . 299 69 69 ARG CA C 59.451 . . 300 69 69 ARG CB C 29.399 . . 301 69 69 ARG N N 120.77 . . 302 70 70 GLN H H 8.48 . . 303 70 70 GLN C C 179.931 . . 304 70 70 GLN CA C 59.108 . . 305 70 70 GLN CB C 28.274 . . 306 70 70 GLN N N 119.829 . . 307 71 71 ASN H H 8.892 . . 308 71 71 ASN C C 177.94 . . 309 71 71 ASN CA C 55.367 . . 310 71 71 ASN CB C 36.615 . . 311 71 71 ASN N N 120.391 . . 312 72 72 ALA H H 7.814 . . 313 72 72 ALA C C 181.257 . . 314 72 72 ALA CA C 54.967 . . 315 72 72 ALA CB C 17.106 . . 316 72 72 ALA N N 122.873 . . 317 73 73 LEU H H 7.456 . . 318 73 73 LEU C C 180.48 . . 319 73 73 LEU CA C 57.67 . . 320 73 73 LEU CB C 40.947 . . 321 73 73 LEU N N 119.324 . . 322 74 74 LEU H H 7.963 . . 323 74 74 LEU C C 179.571 . . 324 74 74 LEU CA C 57.946 . . 325 74 74 LEU CB C 42.466 . . 326 74 74 LEU N N 120.855 . . 327 75 75 GLU H H 8.695 . . 328 75 75 GLU C C 180.758 . . 329 75 75 GLU CA C 59.612 . . 330 75 75 GLU CB C 28.822 . . 331 75 75 GLU N N 118.687 . . 332 76 76 GLN H H 7.553 . . 333 76 76 GLN C C 178.799 . . 334 76 76 GLN CA C 58.461 . . 335 76 76 GLN CB C 27.838 . . 336 76 76 GLN N N 117.512 . . 337 77 77 GLN H H 7.787 . . 338 77 77 GLN C C 179.071 . . 339 77 77 GLN CA C 58.451 . . 340 77 77 GLN CB C 28.766 . . 341 77 77 GLN N N 118.862 . . 342 78 78 VAL H H 8.114 . . 343 78 78 VAL C C 177.812 . . 344 78 78 VAL CA C 64.972 . . 345 78 78 VAL CB C 31.256 . . 346 78 78 VAL N N 117.98 . . 347 79 79 ARG H H 7.63 . . 348 79 79 ARG C C 176.531 . . 349 79 79 ARG CA C 57.471 . . 350 79 79 ARG CB C 29.68 . . 351 79 79 ARG N N 118.49 . . 352 80 80 ALA H H 7.385 . . 353 80 80 ALA C C 177.024 . . 354 80 80 ALA CA C 52.121 . . 355 80 80 ALA CB C 18.737 . . 356 80 80 ALA N N 121.424 . . 357 81 81 LEU H H 7.242 . . 358 81 81 LEU C C 182.583 . . 359 81 81 LEU CA C 56.661 . . 360 81 81 LEU CB C 42.719 . . 361 81 81 LEU N N 126.135 . . stop_ save_