data_27484 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone assignment of KSHV ORF57 68-178 ; _BMRB_accession_number 27484 _BMRB_flat_file_name bmr27484.str _Entry_type original _Submission_date 2018-05-15 _Accession_date 2018-05-15 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details "Backbone assignmnt of Kaposi's Sarcoma herpes virus ORF57 residues 68-178" loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Tunnicliffe Richard B. . 2 Tian Xiaochen . . 3 Storer Joanna . . 4 Sandri-Goldin Rozanne M. . 5 Golovanov Alexander P. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 93 "15N chemical shifts" 93 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-10-10 update BMRB 'update entry citation' 2018-09-24 original author 'original release' stop_ _Original_release_date 2018-05-16 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Overlapping motifs on the herpes viral proteins ICP27 and ORF57 mediate interactions with the mRNA export adaptors ALYREF and UIF ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Tunnicliffe Richard B. . 2 Tian Xiaochen . . 3 Storer Joanna . . 4 Sandri-Goldin Rozanne M. . 5 Golovanov Alexander P. . stop_ _Journal_abbreviation 'Sci. Rep.' _Journal_volume 8 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 15005 _Page_last 15005 _Year 2018 _Details . loop_ _Keyword 'Intrinsically disordered' 'mRNA export' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'ORF57 68-178' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'ORF57 68-178' $ORF57 stop_ _System_molecular_weight 15024.10 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function 'Interaction with AlyREF, UIF and RNA' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_ORF57 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common ORF57 _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'Accumilation and nuclear export of viral mRNA' 'Splicing modulation' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 132 _Mol_residue_sequence ; MGSSHHHHHHSSGLVPRGSH MGRIPKKSQGRSQLRSEIQF CSPLSRPRSPSPVNRYGKKI KFGTAGQNTRPPPEKRPRRR PRDRLQYGRTTRGGQCRAAP KRATRRPQVNCQRQDDDVRQ GVSDAVKKLRLP ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 47 MET 2 48 GLY 3 49 SER 4 50 SER 5 51 HIS 6 52 HIS 7 53 HIS 8 54 HIS 9 55 HIS 10 56 HIS 11 57 SER 12 58 SER 13 59 GLY 14 60 LEU 15 61 VAL 16 62 PRO 17 63 ARG 18 64 GLY 19 65 SER 20 66 HIS 21 67 MET 22 68 GLY 23 69 ARG 24 70 ILE 25 71 PRO 26 72 LYS 27 73 LYS 28 74 SER 29 75 GLN 30 76 GLY 31 77 ARG 32 78 SER 33 79 GLN 34 80 LEU 35 81 ARG 36 82 SER 37 83 GLU 38 84 ILE 39 85 GLN 40 86 PHE 41 87 CYS 42 88 SER 43 89 PRO 44 90 LEU 45 91 SER 46 92 ARG 47 93 PRO 48 94 ARG 49 95 SER 50 96 PRO 51 97 SER 52 98 PRO 53 99 VAL 54 100 ASN 55 101 ARG 56 102 TYR 57 103 GLY 58 104 LYS 59 105 LYS 60 106 ILE 61 107 LYS 62 108 PHE 63 109 GLY 64 110 THR 65 111 ALA 66 112 GLY 67 113 GLN 68 114 ASN 69 115 THR 70 116 ARG 71 117 PRO 72 118 PRO 73 119 PRO 74 120 GLU 75 121 LYS 76 122 ARG 77 123 PRO 78 124 ARG 79 125 ARG 80 126 ARG 81 127 PRO 82 128 ARG 83 129 ASP 84 130 ARG 85 131 LEU 86 132 GLN 87 133 TYR 88 134 GLY 89 135 ARG 90 136 THR 91 137 THR 92 138 ARG 93 139 GLY 94 140 GLY 95 141 GLN 96 142 CYS 97 143 ARG 98 144 ALA 99 145 ALA 100 146 PRO 101 147 LYS 102 148 ARG 103 149 ALA 104 150 THR 105 151 ARG 106 152 ARG 107 153 PRO 108 154 GLN 109 155 VAL 110 156 ASN 111 157 CYS 112 158 GLN 113 159 ARG 114 160 GLN 115 161 ASP 116 162 ASP 117 163 ASP 118 164 VAL 119 165 ARG 120 166 GLN 121 167 GLY 122 168 VAL 123 169 SER 124 170 ASP 125 171 ALA 126 172 VAL 127 173 LYS 128 174 LYS 129 175 LEU 130 176 ARG 131 177 LEU 132 178 PRO stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value UNP Q2HR75 ORF57 . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain _Gene_mnemonic _Details $ORF57 'Human gammaherpesvirus 8' 37296 Viruses . Rhadinovirus 'Human herpesvirus 8 type P' GK18 ORF57 "Kaposi's sarcoma herpes virus" stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $ORF57 'recombinant technology' . Escherichia coli 'T7 express' pET-15b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'ORF57 68-178' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $ORF57 0.4 mM '[U-100% 15N]' 'sodium phosphate' 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' L-Arginine 50 mM 'natural abundance' L-Glutamate 50 mM 'natural abundance' EDTA 1 mM 'natural abundance' TCEP 2 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version 3.2 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version 3.12 loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_800 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_600 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_1H-15N_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N TOCSY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.15 . M pH 6.2 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $SPARKY stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D 1H-15N NOESY' '3D 1H-15N TOCSY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'ORF57 68-178' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 59 13 GLY H H 8.321 0.010 1 2 59 13 GLY N N 110.604 0.050 1 3 60 14 LEU H H 7.964 0.010 1 4 60 14 LEU N N 121.539 0.050 1 5 61 15 VAL H H 8.016 0.010 1 6 61 15 VAL N N 122.743 0.050 1 7 64 18 GLY H H 8.423 0.010 1 8 64 18 GLY N N 110.361 0.050 1 9 65 19 SER H H 8.153 0.010 1 10 65 19 SER N N 115.619 0.050 1 11 68 22 GLY H H 8.324 0.010 1 12 68 22 GLY N N 109.874 0.050 1 13 69 23 ARG H H 8.050 0.010 1 14 69 23 ARG N N 120.528 0.050 1 15 70 24 ILE H H 8.179 0.010 1 16 70 24 ILE N N 124.279 0.050 1 17 72 26 LYS H H 8.314 0.010 1 18 72 26 LYS N N 122.236 0.050 1 19 74 28 SER H H 8.359 0.010 1 20 74 28 SER N N 117.818 0.050 1 21 76 30 GLY H H 8.360 0.010 1 22 76 30 GLY N N 110.018 0.050 1 23 77 31 ARG H H 8.218 0.010 1 24 77 31 ARG N N 120.724 0.050 1 25 78 32 SER H H 8.199 0.010 1 26 78 32 SER N N 116.637 0.050 1 27 79 33 GLN H H 8.385 0.010 1 28 79 33 GLN N N 112.401 0.050 1 29 80 34 LEU H H 8.097 0.010 1 30 80 34 LEU N N 120.767 0.050 1 31 81 35 ARG H H 8.278 0.010 1 32 81 35 ARG N N 122.021 0.050 1 33 82 36 SER H H 8.300 0.010 1 34 82 36 SER N N 117.379 0.050 1 35 83 37 GLU H H 8.386 0.010 1 36 83 37 GLU N N 122.735 0.050 1 37 84 38 ILE H H 7.946 0.010 1 38 84 38 ILE N N 121.279 0.050 1 39 85 39 GLN H H 8.208 0.010 1 40 85 39 GLN N N 123.787 0.050 1 41 86 40 PHE H H 8.189 0.010 1 42 86 40 PHE N N 121.616 0.050 1 43 87 41 CYS H H 8.091 0.010 1 44 87 41 CYS N N 121.153 0.050 1 45 88 42 SER H H 8.291 0.010 1 46 88 42 SER N N 119.708 0.050 1 47 90 44 LEU H H 8.139 0.010 1 48 90 44 LEU N N 123.249 0.050 1 49 91 45 SER H H 8.014 0.010 1 50 91 45 SER N N 116.190 0.050 1 51 92 46 ARG H H 8.080 0.010 1 52 92 46 ARG N N 123.683 0.050 1 53 95 49 SER H H 8.320 0.010 1 54 95 49 SER N N 118.749 0.050 1 55 97 51 SER H H 8.339 0.010 1 56 97 51 SER N N 117.970 0.050 1 57 99 53 VAL H H 8.026 0.010 1 58 99 53 VAL N N 119.383 0.050 1 59 100 54 ASN H H 8.354 0.010 1 60 100 54 ASN N N 122.134 0.050 1 61 101 55 ARG H H 8.225 0.010 1 62 101 55 ARG N N 121.520 0.050 1 63 102 56 TYR H H 8.027 0.010 1 64 102 56 TYR N N 119.746 0.050 1 65 103 57 GLY H H 8.091 0.010 1 66 103 57 GLY N N 109.593 0.050 1 67 104 58 LYS H H 7.972 0.010 1 68 104 58 LYS N N 120.751 0.050 1 69 105 59 LYS H H 8.307 0.010 1 70 105 59 LYS N N 123.077 0.050 1 71 106 60 ILE H H 8.011 0.010 1 72 106 60 ILE N N 122.844 0.050 1 73 107 61 LYS H H 8.198 0.010 1 74 107 61 LYS N N 125.740 0.050 1 75 108 62 PHE H H 8.239 0.010 1 76 108 62 PHE N N 122.309 0.050 1 77 109 63 GLY H H 8.278 0.010 1 78 109 63 GLY N N 110.810 0.050 1 79 110 64 THR H H 7.995 0.010 1 80 110 64 THR N N 113.483 0.050 1 81 111 65 ALA H H 8.322 0.010 1 82 111 65 ALA N N 126.388 0.050 1 83 112 66 GLY H H 8.297 0.010 1 84 112 66 GLY N N 108.244 0.050 1 85 113 67 GLN H H 8.107 0.010 1 86 113 67 GLN N N 119.565 0.050 1 87 114 68 ASN H H 8.462 0.010 1 88 114 68 ASN N N 119.714 0.050 1 89 115 69 THR H H 7.994 0.010 1 90 115 69 THR N N 114.477 0.050 1 91 116 70 ARG H H 8.221 0.010 1 92 116 70 ARG N N 124.622 0.050 1 93 122 76 ARG H H 8.240 0.010 1 94 122 76 ARG N N 123.369 0.050 1 95 124 78 ARG H H 8.415 0.010 1 96 124 78 ARG N N 121.686 0.050 1 97 125 79 ARG H H 8.384 0.010 1 98 125 79 ARG N N 123.022 0.050 1 99 126 80 ARG H H 8.471 0.010 1 100 126 80 ARG N N 124.219 0.050 1 101 128 82 ARG H H 8.401 0.010 1 102 128 82 ARG N N 120.615 0.050 1 103 129 83 ASP H H 8.178 0.010 1 104 129 83 ASP N N 121.243 0.050 1 105 130 84 ARG H H 8.186 0.010 1 106 130 84 ARG N N 121.243 0.050 1 107 131 85 LEU H H 8.133 0.010 1 108 131 85 LEU N N 122.362 0.050 1 109 132 86 GLN H H 8.109 0.010 1 110 132 86 GLN N N 120.091 0.050 1 111 133 87 TYR H H 8.071 0.010 1 112 133 87 TYR N N 120.911 0.050 1 113 134 88 GLY H H 8.243 0.010 1 114 134 88 GLY N N 110.211 0.050 1 115 135 89 ARG H H 8.095 0.010 1 116 135 89 ARG N N 120.478 0.050 1 117 136 90 THR H H 8.210 0.010 1 118 136 90 THR N N 115.049 0.050 1 119 137 91 THR H H 8.120 0.010 1 120 137 91 THR N N 116.495 0.050 1 121 138 92 ARG H H 8.355 0.010 1 122 138 92 ARG N N 123.523 0.050 1 123 139 93 GLY H H 8.393 0.010 1 124 139 93 GLY N N 110.260 0.050 1 125 140 94 GLY H H 8.221 0.010 1 126 140 94 GLY N N 108.727 0.050 1 127 141 95 GLN H H 8.229 0.010 1 128 141 95 GLN N N 119.761 0.050 1 129 142 96 CYS H H 8.364 0.010 1 130 142 96 CYS N N 120.830 0.050 1 131 144 98 ALA H H 8.213 0.010 1 132 144 98 ALA N N 125.632 0.050 1 133 145 99 ALA H H 8.201 0.010 1 134 145 99 ALA N N 125.205 0.050 1 135 149 103 ALA H H 8.358 0.010 1 136 149 103 ALA N N 126.057 0.050 1 137 150 104 THR H H 8.107 0.010 1 138 150 104 THR N N 114.589 0.050 1 139 151 105 ARG H H 8.319 0.010 1 140 151 105 ARG N N 124.075 0.050 1 141 152 106 ARG H H 8.383 0.010 1 142 152 106 ARG N N 124.135 0.050 1 143 154 108 GLN H H 8.424 0.010 1 144 154 108 GLN N N 121.153 0.050 1 145 155 109 VAL H H 8.099 0.010 1 146 155 109 VAL N N 121.290 0.050 1 147 156 110 ASN H H 8.494 0.010 1 148 156 110 ASN N N 122.212 0.050 1 149 159 113 ARG H H 8.412 0.010 1 150 159 113 ARG N N 122.085 0.050 1 151 160 114 GLN H H 8.484 0.010 1 152 160 114 GLN N N 122.273 0.050 1 153 161 115 ASP H H 8.317 0.010 1 154 161 115 ASP N N 121.440 0.050 1 155 162 116 ASP H H 8.185 0.010 1 156 162 116 ASP N N 120.378 0.050 1 157 163 117 ASP H H 8.300 0.010 1 158 163 117 ASP N N 122.700 0.050 1 159 164 118 VAL H H 7.896 0.010 1 160 164 118 VAL N N 120.182 0.050 1 161 165 119 ARG H H 8.198 0.010 1 162 165 119 ARG N N 123.699 0.050 1 163 166 120 GLN H H 8.243 0.010 1 164 166 120 GLN N N 120.807 0.050 1 165 167 121 GLY H H 8.334 0.010 1 166 167 121 GLY N N 110.107 0.050 1 167 168 122 VAL H H 7.884 0.010 1 168 168 122 VAL N N 119.035 0.050 1 169 169 123 SER H H 8.310 0.010 1 170 169 123 SER N N 119.129 0.050 1 171 170 124 ASP H H 8.166 0.010 1 172 170 124 ASP N N 120.231 0.050 1 173 171 125 ALA H H 8.047 0.010 1 174 171 125 ALA N N 123.221 0.050 1 175 172 126 VAL H H 7.799 0.010 1 176 172 126 VAL N N 118.458 0.050 1 177 173 127 LYS H H 8.091 0.010 1 178 173 127 LYS N N 124.091 0.050 1 179 174 128 LYS H H 8.047 0.010 1 180 174 128 LYS N N 121.633 0.050 1 181 175 129 LEU H H 7.971 0.010 1 182 175 129 LEU N N 122.939 0.050 1 183 176 130 ARG H H 8.119 0.010 1 184 176 130 ARG N N 122.110 0.050 1 185 177 131 LEU H H 8.145 0.010 1 186 177 131 LEU N N 126.015 0.050 1 stop_ save_