data_27466 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Chemical shift assignments of the C-terminal domain of human FAT10 ; _BMRB_accession_number 27466 _BMRB_flat_file_name bmr27466.str _Entry_type original _Submission_date 2018-04-25 _Accession_date 2018-04-25 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details fat10_c loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Aichem Annette . . 2 Anders Samira . . 3 Catone Nicola . . 4 Roessler Philip . . 5 Stotz Sophie . . 6 Berg Andrej . . 7 Schwab Ricarda . . 8 Scheuermann Sophia . . 9 Bialas Johanna . . 10 Schmidtke Gunter . . 11 Peter Christine . . 12 Groettrup Marcus . . 13 Wiesner Silke . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 5 "13C chemical shifts" 6 "15N chemical shifts" 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-11-07 update BMRB 'update entry citation' 2018-07-31 original author 'original release' stop_ _Original_release_date 2018-04-25 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; The structure of the ubiquitin-like modifier FAT10 reveals an alternative targeting mechanism for proteasomal degradation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 30127417 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Aichem Annette . . 2 Anders Samira . . 3 Catone Nicola . . 4 Rossler Philip . . 5 Stotz Sophie . . 6 Berg Andrej . . 7 Schwab Ricarda . . 8 Scheuermann Sophia . . 9 Bialas Johanna . . 10 Schutz-Stoffregen Mira C. . 11 Schmidtke Gunter . . 12 Peter Christine . . 13 Groettrup Marcus . . 14 Wiesner Silke . . stop_ _Journal_abbreviation 'Nat. Commun.' _Journal_name_full 'Nature communications' _Journal_volume 9 _Journal_issue 1 _Journal_ISSN 2041-1723 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 3321 _Page_last 3321 _Year 2018 _Details . loop_ _Keyword FAT10 degradation proteasome 'ubiquitin-like modifier' vcp stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'FAT10 C-terminal domain' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'FAT10 C-terminal domain' $fat10_c stop_ _System_molecular_weight 9251.70 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Magnetic_equivalence_ID _Magnetically_equivalent_system_component 1 'FAT10 C-terminal domain' stop_ _Database_query_date . _Details Monomer save_ ######################## # Monomeric polymers # ######################## save_fat10_c _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common fat10_c _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'proteasomal degradation' 'protein modifiaction' stop_ _Details 'The construct contains four additional amino acids (GAMG) as a result of the NcoI restriction site.' ############################## # Polymer residue sequence # ############################## _Residue_count 85 _Mol_residue_sequence ; GAMGDEELPLFLVESGDEAK RHLLQVRRSSSVAQVKAMIE TKTGIIPETQIVTLNGKRLE DGKMMADYGIRKGNLLFLAS YSIGG ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 ALA 3 MET 4 GLY 5 ASP 6 GLU 7 GLU 8 LEU 9 PRO 10 LEU 11 PHE 12 LEU 13 VAL 14 GLU 15 SER 16 GLY 17 ASP 18 GLU 19 ALA 20 LYS 21 ARG 22 HIS 23 LEU 24 LEU 25 GLN 26 VAL 27 ARG 28 ARG 29 SER 30 SER 31 SER 32 VAL 33 ALA 34 GLN 35 VAL 36 LYS 37 ALA 38 MET 39 ILE 40 GLU 41 THR 42 LYS 43 THR 44 GLY 45 ILE 46 ILE 47 PRO 48 GLU 49 THR 50 GLN 51 ILE 52 VAL 53 THR 54 LEU 55 ASN 56 GLY 57 LYS 58 ARG 59 LEU 60 GLU 61 ASP 62 GLY 63 LYS 64 MET 65 MET 66 ALA 67 ASP 68 TYR 69 GLY 70 ILE 71 ARG 72 LYS 73 GLY 74 ASN 75 LEU 76 LEU 77 PHE 78 LEU 79 ALA 80 SER 81 TYR 82 SER 83 ILE 84 GLY 85 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value GB AAD52982.1 'ubiquitin-like protein FAT10' . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $fat10_c Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Variant _Vector_name $fat10_c 'recombinant technology' . Escherichia coli BL21(DE3) CodonPlus pETM-41 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $fat10_c 2 mM '[U-100% 13C; U-100% 15N]' 'sodium chloride' 150 mM 'natural abundance' 'sodium phosphate' 20 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_XEASY _Saveframe_category software _Name XEASY _Version . loop_ _Vendor _Address _Electronic_address '(Xeasy) W thrich' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details 'Chemical shift assignment' save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details 'NOESY spectra' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_C(CO)NH_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D C(CO)NH' _Sample_label $sample_1 save_ save_3D_HBHA(CO)NH_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HBHA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNHA_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNHA' _Sample_label $sample_1 save_ save_3D_HCCH-TOCSY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCCH-TOCSY' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_1H-13C_NOESY_10 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_11 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.2 . M pH 7.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 0 na direct . . . . water H 1 protons ppm 4.78 internal direct . . . 1 water N 15 protons ppm 0 na direct . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $XEASY stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '2D 1H-13C HSQC' '3D HNCACB' '3D CBCA(CO)NH' '3D C(CO)NH' '3D HBHA(CO)NH' '3D HNHA' '3D HCCH-TOCSY' '3D 1H-15N NOESY' '3D 1H-13C NOESY' '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'FAT10 C-terminal domain' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 81 81 TYR CA C 43.745 0.000 1 2 82 82 SER HA H 4.432 0.000 1 3 82 82 SER HB2 H 1.457 0.000 1 4 82 82 SER HB3 H 1.457 0.000 1 5 82 82 SER CA C 52.566 0.000 1 6 82 82 SER CB C 19.107 0.000 1 7 83 83 ILE HA H 4.532 0.000 1 8 83 83 ILE CA C 55.770 0.000 1 9 83 83 ILE CB C 32.546 0.000 1 10 84 84 GLY H H 8.545 0.000 1 11 84 84 GLY N N 110.530 0.000 1 12 85 85 GLY CA C 53.485 0.000 1 stop_ save_