data_25052 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; A structure-based mechanism for tRNA and retroviral RNA remodeling during primer annealing ; _BMRB_accession_number 25052 _BMRB_flat_file_name bmr25052.str _Entry_type original _Submission_date 2015-10-16 _Accession_date 2015-10-16 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 D'Souza Victoria M. Prof 2 Yildiz Zehra . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 238 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2015-10-16 update BMRB 'update entry citation' 2014-09-15 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 25049 'RNA (68-MER)' 25100 'pf tRNApro:MLV-Nucleocapsid (1:2) Complex' 25101 'tRNApro:MLV Nucleocapsid Protein (1:1) Complex' stop_ _Original_release_date 2015-10-16 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; A structure-based mechanism for tRNA and retroviral RNA remodelling during primer annealing ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 25209668 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Miller Sarah B. . 2 Yildiz F. Zehra . 3 Lo Jennifer A. . 4 Wang Bo . . 5 D'Souza Victoria M. . stop_ _Journal_abbreviation Nature _Journal_volume 515 _Journal_issue 7528 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 591 _Page_last 595 _Year 2014 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Nucleocapsid protein p10 and RNA (68-MER)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $entity_1 'RNA (68-MER)' $RNA_(68-MER) stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass 6377.297 _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 56 _Mol_residue_sequence ; ATVVSGQKQDRQGGERRRSQ LDRDQCAYCKEKGHWAKDCP KKPRGPRGPRPQTSLL ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 ALA 2 2 THR 3 3 VAL 4 4 VAL 5 5 SER 6 6 GLY 7 7 GLN 8 8 LYS 9 9 GLN 10 10 ASP 11 11 ARG 12 12 GLN 13 13 GLY 14 14 GLY 15 15 GLU 16 16 ARG 17 17 ARG 18 18 ARG 19 19 SER 20 20 GLN 21 21 LEU 22 22 ASP 23 23 ARG 24 24 ASP 25 25 GLN 26 26 CYS 27 27 ALA 28 28 TYR 29 29 CYS 30 30 LYS 31 31 GLU 32 32 LYS 33 33 GLY 34 34 HIS 35 35 TRP 36 36 ALA 37 37 LYS 38 38 ASP 39 39 CYS 40 40 PRO 41 41 LYS 42 42 LYS 43 43 PRO 44 44 ARG 45 45 GLY 46 46 PRO 47 47 ARG 48 48 GLY 49 49 PRO 50 50 ARG 51 51 PRO 52 52 GLN 53 53 THR 54 54 SER 55 55 LEU 56 56 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-25 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 25100 MLV_Nucleocapsid 98.21 55 100.00 100.00 4.24e-30 BMRB 25101 MLV_Nucleocapsid 98.21 55 100.00 100.00 4.24e-30 PDB 1A6B "Nmr Structure Of The Complex Between The Zinc Finger Protein Ncp10 Of Moloney Murine Leukemia Virus And A Sequence Of The Psi-P" 71.43 40 100.00 100.00 2.61e-19 PDB 1U6P "Nmr Structure Of The Mlv Encapsidation Signal Bound To The Nucleocapsid Protein" 100.00 56 100.00 100.00 5.64e-31 PDB 1WWD "Nmr Structure Determined For Mlv Nc Complex With Rna Sequence Aacagu" 100.00 56 100.00 100.00 5.64e-31 PDB 1WWE "Nmr Structure Determined For Mlv Nc Complex With Rna Sequence Uuuugcu" 100.00 56 100.00 100.00 5.64e-31 PDB 1WWF "Nmr Structure Determined For Mlv Nc Complex With Rna Sequence Ccuccgu" 100.00 56 100.00 100.00 5.64e-31 PDB 1WWG "Nmr Structure Determined For Mlv Nc Complex With Rna Sequence Uaucug" 100.00 56 100.00 100.00 5.64e-31 PDB 2MQV "Solution Nmr Structure Of The U5-primer Binding Site (u5-pbs) Domain Of Murine Leukemia Virus Rna Genome Bound To The Retrovira" 100.00 56 100.00 100.00 5.64e-31 PDB 2MS0 "Solution Nmr Structure Pf Trnapro:mlv-nucleocapsid (1:2) Complex" 100.00 56 100.00 100.00 5.64e-31 PDB 2MS1 "Solution Nmr Structure Of Trnapro:mlv Nucleocapsid Protein (1:1) Complex" 100.00 56 100.00 100.00 5.64e-31 GB AAB59942 "gag polyprotein pr65 [Murine leukemia virus]" 100.00 538 100.00 100.00 3.80e-29 GB AAB64159 "Gag [synthetic construct]" 100.00 538 100.00 100.00 3.80e-29 GB AAC82566 "Pr65 [Moloney murine leukemia virus]" 100.00 538 100.00 100.00 3.80e-29 GB AAC82568 "Pr180 [Moloney murine leukemia virus]" 100.00 1737 100.00 100.00 1.11e-28 GB AAL69908 "gPr80 glycosylated gag polyprotein [Moloney murine leukemia virus]" 100.00 626 98.21 100.00 1.89e-28 PRF 0711245A "protein gag/pol/env" 100.00 2514 100.00 100.00 1.60e-28 REF NP_057933 "Pr180 [Moloney murine leukemia virus]" 100.00 1737 100.00 100.00 1.11e-28 REF NP_057934 "Pr65 [Moloney murine leukemia virus]" 100.00 538 100.00 100.00 3.80e-29 REF NP_955586 "p10 NC [Moloney murine leukemia virus]" 100.00 56 100.00 100.00 5.64e-31 SP P03332 "RecName: Full=Gag polyprotein; Short=Pr65gag; AltName: Full=Core polyprotein; Contains: RecName: Full=Matrix protein p15; Short" 100.00 538 100.00 100.00 3.80e-29 SP P03355 "RecName: Full=Gag-Pol polyprotein; Short=Pr180gag-pol; Contains: RecName: Full=Matrix protein p15; Short=MA; Contains: RecName:" 100.00 1738 100.00 100.00 1.17e-28 SP Q8UN02 "RecName: Full=Glycosylated Gag polyprotein; Short=Pr80gag; AltName: Full=Glyco-gag; AltName: Full=gp80gag; Contains: RecName: F" 100.00 626 98.21 100.00 1.89e-28 stop_ save_ save_RNA_(68-MER) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common RNA_(68-MER) _Molecular_mass 21894.025 _Mol_thiol_state 'not present' _Details . _Residue_count 68 _Mol_residue_sequence ; GGGCGAGGGUCUCCUCUGAG UGAUUGACUACCCGUCAGCG GGGGUCUUUCAUUUGGGGGC UCGUGCCC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 93 G 2 94 G 3 95 G 4 96 C 5 97 G 6 98 A 7 99 G 8 100 G 9 101 G 10 102 U 11 103 C 12 104 U 13 105 C 14 106 C 15 107 U 16 108 C 17 109 U 18 110 G 19 111 A 20 112 G 21 113 U 22 114 G 23 115 A 24 116 U 25 117 U 26 118 G 27 119 A 28 120 C 29 121 U 30 122 A 31 123 C 32 124 C 33 125 C 34 126 G 35 127 U 36 128 C 37 129 A 38 130 G 39 131 C 40 132 G 41 133 G 42 134 G 43 135 G 44 136 G 45 137 U 46 138 C 47 139 U 48 140 U 49 141 U 50 142 C 51 143 A 52 144 U 53 145 U 54 146 U 55 147 G 56 148 G 57 149 G 58 150 G 59 151 G 60 152 C 61 153 U 62 154 C 63 155 G 64 156 U 65 157 G 66 158 C 67 159 C 68 160 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 'Murine Leukemia Virus' 11786 . . Gammaretrovirus . $RNA_(68-MER) Mouse 10090 Eukaryota Metazoa Mus musculus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'recombinant technology' . . . . pCNA $RNA_(68-MER) 'enzymatic semisynthesis' . . . . 'not applicable' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1-U5-PBS _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM 15N,13C-Alab $entity_1 0.5 mM 15N,13C-Glab $entity_1 0.5 mM 15N,13C-Ulab $entity_1 0.5 mM 15N,13C-Clab NaCl 10 mM 'natural abundance' Tris 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_2-U5-PBS-M2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 0.5 mM 'natural abundance' NaCl 10 mM 'natural abundance' Tris 10 mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ save_sample_3-linker _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1 mM 'natural abundance' NaCl 10 mM 'natural abundance' Tris 10 mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CYANA _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 'Guntert, Mumenthaler and Wuthrich' . . 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' processing refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1-U5-PBS save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1-U5-PBS save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2-U5-PBS-M2 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_3-linker save_ save_3D_1H-13C_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_1-U5-PBS save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.0 . pH pressure 1 . atm temperature 311 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.0 . pH temperature 288 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1-U5-PBS $sample_2-U5-PBS-M2 $sample_3-linker stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 ALA HA H 4.2279 0.0 1 2 1 1 ALA HB H 1.2419 0.0 1 3 2 2 THR HA H 4.10390333333 0.0 1 4 2 2 THR HB H 3.9254 0.0 1 5 2 2 THR HG2 H 0.9809 0.0 1 6 3 3 VAL HA H 3.98891 0.0 1 7 3 3 VAL HB H 1.8759 0.0 1 8 3 3 VAL HG1 H 0.780223333333 0.0 2 9 4 4 VAL HA H 4.00691 0.0 1 10 4 4 VAL HB H 1.91491 0.0 1 11 4 4 VAL HG1 H 0.77829 0.0 2 12 5 5 SER HA H 4.29491 0.0 1 13 5 5 SER HB2 H 3.74591 0.0 2 14 6 6 GLY HA2 H 3.80041 0.0 2 15 7 7 GLN HA H 4.19591 0.0 1 16 7 7 GLN HB2 H 1.82591 0.0 2 17 7 7 GLN HG2 H 2.15189666667 0.0 2 18 8 8 LYS HA H 4.12591 0.0 1 19 8 8 LYS HE2 H 2.84891 0.0 2 20 18 18 ARG HA H 4.02322333333 0.0 1 21 18 18 ARG HB2 H 1.54522333333 0.0 2 22 18 18 ARG HG2 H 1.38839 0.0 2 23 18 18 ARG HD2 H 2.94289 0.0 2 24 19 19 SER HA H 4.24391 0.0 1 25 19 19 SER HB2 H 3.6224 0.0 2 26 21 21 LEU HA H 4.25091 0.0 1 27 21 21 LEU HG H 1.35291 0.0 1 28 21 21 LEU HD1 H 0.376048947368 0.0 2 29 22 22 ASP HA H 4.59091 0.0 1 30 22 22 ASP HB2 H 2.59591 0.0 2 31 23 23 ARG HA H 4.20689 0.0 1 32 23 23 ARG HB2 H 1.74089 0.0 2 33 23 23 ARG HD2 H 3.13289 0.0 2 34 24 24 ASP HA H 4.64289 0.0 1 35 24 24 ASP HB2 H 2.51489 0.0 2 36 26 26 CYS HA H 4.3749 0.0 1 37 26 26 CYS HB2 H 2.32439 0.0 2 38 27 27 ALA HA H 3.74391 0.0 1 39 27 27 ALA HB H 0.8980425 0.0 1 40 28 28 TYR HA H 4.25455666667 0.0 1 41 28 28 TYR HB2 H 3.08489 0.0 2 42 28 28 TYR HD2 H 6.82813 0.0 3 43 28 28 TYR HE2 H 6.39527421053 0.0 3 44 29 29 CYS HA H 4.14289 0.0 1 45 29 29 CYS HB2 H 2.39889 0.0 2 46 30 30 LYS HA H 4.15391 0.0 1 47 30 30 LYS HE2 H 2.612898 0.0 2 48 31 31 GLU HA H 4.26841 0.0 1 49 31 31 GLU HB2 H 1.98089 0.0 2 50 31 31 GLU HG2 H 2.294895 0.0 2 51 34 34 HIS HA H 4.12628 0.0 1 52 34 34 HIS HB2 H 3.07273 0.0 2 53 34 34 HIS HB3 H 3.30868333333 0.0 2 54 34 34 HIS HD1 H 7.09273444444 0.0 1 55 34 34 HIS HE1 H 7.53271 0.0 1 56 35 35 TRP HA H 5.3486525 0.0 1 57 35 35 TRP HB2 H 3.18489 0.0 2 58 35 35 TRP HD1 H 6.58254454545 0.0 1 59 35 35 TRP HE3 H 6.82521307692 0.0 1 60 35 35 TRP HZ2 H 7.25081727273 0.0 1 61 35 35 TRP HZ3 H 6.61544818182 0.0 1 62 35 35 TRP HH2 H 6.94840333333 0.0 1 63 36 36 ALA HB H 1.52289 0.0 1 64 37 37 LYS HA H 3.97389 0.0 1 65 37 37 LYS HB2 H 1.83555666667 0.0 2 66 37 37 LYS HG2 H 1.28564 0.0 2 67 37 37 LYS HG3 H 1.61564 0.0 2 68 37 37 LYS HE2 H 2.81214 0.0 2 69 38 38 ASP HA H 4.92935444444 0.0 1 70 38 38 ASP HB2 H 2.63039 0.0 2 71 39 39 CYS HB2 H 3.13889 0.0 2 72 39 39 CYS HB3 H 3.03089 0.0 2 73 41 41 LYS HB2 H 1.81139 0.0 2 74 41 41 LYS HE2 H 2.89189 0.0 2 75 42 42 LYS HA H 3.71091 0.0 1 76 42 42 LYS HG2 H 0.60249 0.0 2 77 42 42 LYS HD2 H 1.13789 0.0 2 78 42 42 LYS HD3 H 1.26389 0.0 2 79 42 42 LYS HE2 H 2.480895 0.0 2 80 45 45 GLY HA2 H 3.65823 0.0 2 81 46 46 PRO HA H 4.45491 0.0 1 82 54 54 SER HA H 4.33891 0.0 1 83 54 54 SER HB2 H 3.7139 0.0 2 84 55 55 LEU HA H 4.24191 0.0 1 85 55 55 LEU HB2 H 1.48989 0.0 2 86 55 55 LEU HG H 1.48990333333 0.0 1 87 55 55 LEU HD1 H 0.77889 0.0 2 88 55 55 LEU HD1 H 0.71589 0.0 2 89 55 55 LEU HD1 H 0.77889 0.0 2 stop_ save_ save_assigned_chem_shift_list_1_dup _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1-U5-PBS $sample_2-U5-PBS-M2 $sample_3-linker stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'RNA (68-MER)' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 93 1 G H1' H 5.84717 0.0 1 2 93 1 G H2' H 4.94586 0.0 1 3 94 2 G H1' H 5.9372 0.0 1 4 94 2 G H8 H 7.57772 0.0 1 5 95 3 G H1' H 5.80265 0.0 1 6 95 3 G H8 H 7.29898 0.0 1 7 96 4 C H1' H 5.53776 0.0 1 8 96 4 C H6 H 7.41986 0.0 1 9 97 5 G H1' H 5.70456 0.0 1 10 97 5 G H8 H 7.5744 0.0 1 11 98 6 A H1' H 5.97883 0.0 1 12 98 6 A H8 H 7.91785 0.0 1 13 99 7 G H1' H 5.78795 0.0 1 14 99 7 G H8 H 7.34222 0.0 1 15 100 8 G H8 H 7.81571 0.0 1 16 101 9 G H1' H 5.769905 0.0 1 17 101 9 G H8 H 7.20656 0.0 1 18 102 10 U H5 H 5.48451 0.0 1 19 102 10 U H6 H 7.71669 0.0 1 20 104 12 U H5 H 5.70804 0.0 1 21 104 12 U H6 H 7.96503 0.0 1 22 105 13 C H1' H 5.56143 0.0 1 23 105 13 C H6 H 7.99306 0.0 1 24 107 15 U H1' H 5.63732 0.0 1 25 107 15 U H2' H 3.16464 0.0 1 26 107 15 U H3' H 4.30189 0.0 1 27 107 15 U H5 H 5.76288 0.0 1 28 107 15 U H5' H 4.18539 0.0 2 29 107 15 U H5'' H 4.05222333333 0.0 2 30 107 15 U H6 H 7.86284 0.0 1 31 108 16 C H1' H 5.92684533333 0.0 1 32 108 16 C H2' H 4.14489 0.0 1 33 108 16 C H3' H 4.38222333333 0.0 1 34 108 16 C H4' H 4.59555666667 0.0 1 35 108 16 C H5 H 6.19467 0.0 1 36 108 16 C H5' H 3.78839 0.0 2 37 108 16 C H5'' H 3.84322333333 0.0 2 38 108 16 C H6 H 7.68798 0.0 1 39 109 17 U H1' H 5.86533125 0.0 1 40 109 17 U H2' H 3.89071 0.0 1 41 109 17 U H3' H 4.19935333333 0.0 1 42 109 17 U H4' H 4.14186333333 0.0 1 43 109 17 U H5 H 5.743385 0.0 1 44 109 17 U H6 H 7.626285 0.0 1 45 110 18 G H1' H 5.225898 0.0 1 46 110 18 G H2' H 4.49085 0.0 1 47 110 18 G H3' H 4.40081666667 0.0 1 48 110 18 G H4' H 4.03156 0.0 1 49 110 18 G H5' H 3.75289 0.0 2 50 110 18 G H5'' H 3.90189 0.0 2 51 110 18 G H8 H 7.71100809524 0.0 1 52 111 19 A H1' H 5.9469 0.0 1 53 111 19 A H2 H 7.752945 0.0 1 54 111 19 A H8 H 8.26261 0.0 1 55 112 20 G H1' H 5.62382 0.0 1 56 112 20 G H8 H 7.41987 0.0 1 57 113 21 U H5 H 5.1924 0.0 1 58 113 21 U H6 H 7.68825 0.0 1 59 114 22 G H1' H 5.83813 0.0 1 60 114 22 G H8 H 7.71882 0.0 1 61 115 23 A H1' H 5.868665 0.0 1 62 115 23 A H2 H 7.8838075 0.0 1 63 115 23 A H8 H 7.57661 0.0 1 64 116 24 U H1' H 5.243156 0.0 1 65 116 24 U H5 H 5.19752 0.0 1 66 116 24 U H6 H 7.273905 0.0 1 67 117 25 U H6 H 7.76802 0.0 1 68 119 27 A H1' H 6.02524 0.0 1 69 119 27 A H2 H 7.86049 0.0 1 70 119 27 A H8 H 7.864365 0.0 1 71 120 28 C H1' H 5.40529666667 0.0 1 72 120 28 C H5 H 5.12250666667 0.0 1 73 120 28 C H6 H 7.329225 0.0 1 74 121 29 U H1' H 5.863685 0.0 1 75 121 29 U H5 H 5.62174 0.0 1 76 121 29 U H6 H 7.7777675 0.0 1 77 122 30 A H1' H 6.16545 0.0 1 78 122 30 A H2 H 8.23256 0.0 1 79 122 30 A H8 H 8.3943225 0.0 1 80 123 31 C H1' H 5.35488 0.0 1 81 123 31 C H5 H 5.896815 0.0 1 82 123 31 C H6 H 7.97011 0.0 1 83 124 32 C H5 H 5.54369 0.0 1 84 124 32 C H6 H 7.88761666667 0.0 1 85 125 33 C H1' H 5.51073 0.0 1 86 125 33 C H5 H 5.48276 0.0 1 87 125 33 C H6 H 7.75864 0.0 1 88 126 34 G H1' H 5.70452 0.0 1 89 126 34 G H8 H 7.494275 0.0 1 90 127 35 U H5 H 5.416735 0.0 1 91 127 35 U H6 H 7.56636 0.0 1 92 128 36 C H1' H 6.044535 0.0 1 93 128 36 C H5 H 6.013335 0.0 1 94 128 36 C H6 H 7.97168 0.0 1 95 129 37 A H1' H 5.8797 0.0 1 96 129 37 A H8 H 8.14492 0.0 1 97 130 38 G H1' H 5.9302 0.0 1 98 130 38 G H8 H 7.87534 0.0 1 99 131 39 C H5 H 5.986365 0.0 1 100 131 39 C H6 H 8.0636 0.0 1 101 132 40 G H1' H 5.68891 0.0 1 102 132 40 G H8 H 7.61964 0.0 1 103 133 41 G H1' H 5.75118 0.0 1 104 133 41 G H8 H 7.21821 0.0 1 105 134 42 G H1' H 5.763875 0.0 1 106 134 42 G H8 H 7.028285 0.0 1 107 135 43 G H1' H 5.6885 0.0 1 108 135 43 G H8 H 7.311965 0.0 1 109 136 44 G H1' H 5.59636 0.0 1 110 136 44 G H8 H 7.299045 0.0 1 111 137 45 U H5 H 5.062085 0.0 1 112 137 45 U H6 H 7.73855 0.0 1 113 140 48 U H1' H 5.350145 0.0 1 114 140 48 U H5 H 5.63416 0.0 1 115 140 48 U H6 H 7.86263 0.0 1 116 141 49 U H1' H 5.7525 0.0 1 117 141 49 U H5 H 5.80082666667 0.0 1 118 141 49 U H6 H 8.0371525 0.0 1 119 142 50 C H1' H 5.5732675 0.0 1 120 142 50 C H5 H 5.71788 0.0 1 121 142 50 C H6 H 7.7585 0.0 1 122 143 51 A H1' H 5.9342675 0.0 1 123 143 51 A H2 H 7.39729 0.0 1 124 143 51 A H8 H 8.02108 0.0 1 125 144 52 U H1' H 5.39731 0.0 1 126 144 52 U H5 H 5.40097 0.0 1 127 144 52 U H6 H 7.56261 0.0 1 128 145 53 U H6 H 7.77924 0.0 1 129 149 57 G H1' H 5.81656 0.0 1 130 149 57 G H8 H 7.16927 0.0 1 131 150 58 G H1' H 5.826025 0.0 1 132 150 58 G H8 H 7.30454333333 0.0 1 133 151 59 G H1' H 5.78529 0.0 1 134 151 59 G H8 H 7.19506 0.0 1 135 152 60 C H1' H 5.5686 0.0 1 136 152 60 C H5 H 5.295455 0.0 1 137 152 60 C H6 H 7.5831625 0.0 1 138 153 61 U H5 H 5.7522 0.0 1 139 153 61 U H6 H 7.864525 0.0 1 140 154 62 C H1' H 5.76935 0.0 1 141 154 62 C H6 H 7.835815 0.0 1 142 156 64 U H1' H 5.668465 0.0 1 143 156 64 U H5 H 5.29315 0.0 1 144 156 64 U H6 H 7.68121 0.0 1 145 157 65 G H1' H 5.67335 0.0 1 146 157 65 G H8 H 7.99177 0.0 1 147 158 66 C H1' H 5.70507 0.0 1 148 158 66 C H5 H 5.26585 0.0 1 149 158 66 C H6 H 7.747885 0.0 1 stop_ save_