# 1 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcf3gpsi3d.nus"
;noesyhsqcf3gpsi3d
;avance-version (07/04/04)
;NOESY-HSQC
;3D sequence with
;   homonuclear correlation via dipolar coupling
;   dipolar coupling may be due to noe or chemical exchange.
;   H-1/X correlation via double inept transfer
;      using sensitivity improvement
;phase sensitive (t1)
;phase sensitive using Echo/Antiecho-TPPI gradient selection (t2)
;with decoupling during acquisition
;using trim pulses in inept transfer
;using f3 - channel
;(use parameterset NOESYHSQCF3GPSI3D)
;
;A.G. Palmer III, J. Cavanagh, P.E. Wright & M. Rance, J. Magn.
;   Reson. 93, 151-170 (1991)
;L.E. Kay, P. Keifer & T. Saarinen, J. Am. Chem. Soc. 114,
;   10663-5 (1992)
;J. Schleucher, M. Schwendinger, M. Sattler, P. Schmidt, O. Schedletzky,
;   S.J. Glaser, O.W. Sorensen & C. Griesinger, J. Biomol. NMR 4,
;   301-306 (1994)
;
;$CLASS=HighRes
;$DIM=3D
;$TYPE=
;$SUBTYPE=
;$COMMENT=


# 1 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/Avance.incl" 1
;Avance3.incl
;   for AV III
;
;avance-version (07/12/14)
;
;$CLASS=HighRes Incl
;$COMMENT=





























































































































































;$Id: pulseprogram,v 1.1 2021/02/11 16:17:33 bmrbsvc Exp $
# 31 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcf3gpsi3d.nus" 2

# 1 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/Grad.incl" 1
;Grad2.incl  -  include file for Gradient Spectroscopy
;   for 1
;
;avance-version (07/01/17)
;
;$CLASS=HighRes Incl
;$COMMENT=



















define list<gradient> EA=<EA>



;$Id: pulseprogram,v 1.1 2021/02/11 16:17:33 bmrbsvc Exp $
# 32 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcf3gpsi3d.nus" 2

# 1 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/Delay.incl" 1
;Delay.incl  -  include file for commonly used delays
;
;version 00/02/07
;
;$CLASS=HighRes Incl
;$COMMENT=


;general delays

define delay DELTA
define delay DELTA1
define delay DELTA2
define delay DELTA3
define delay DELTA4
define delay DELTA5
define delay DELTA6
define delay DELTA7
define delay DELTA8

define delay TAU
define delay TAU1
define delay TAU2
define delay TAU3
define delay TAU4
define delay TAU5


;delays for centering pulses

define delay CEN_HN1
define delay CEN_HN2
define delay CEN_HN3
define delay CEN_HC1
define delay CEN_HC2
define delay CEN_HC3
define delay CEN_HC4
define delay CEN_HP1
define delay CEN_HP2
define delay CEN_CN1
define delay CEN_CN2
define delay CEN_CN3
define delay CEN_CN4
define delay CEN_CP1
define delay CEN_CP2


;loop counters

define loopcounter COUNTER
define loopcounter SCALEF
define loopcounter FACTOR1
define loopcounter FACTOR2
define loopcounter FACTOR3



;$Id: pulseprogram,v 1.1 2021/02/11 16:17:33 bmrbsvc Exp $
# 33 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcf3gpsi3d.nus" 2


;********Added for NUS*******
define loopcounter SPARSEFLAG
define loopcounter SPARSELEV
define loopcounter SPARSESEED
"SPARSEFLAG=cnst60"
"SPARSELEV=cnst61"
"SPARSESEED=cnst62"
;cnst60: = sparse flag (0=yes, 1=no)
;cnst61: = level of sparsing in %
;cnst62: = random seed (usu 709)
define delay short
"short=50u"
"l2=td2/2"
"l3=td1/2"
;^^^^^^^^^^^^^^^^^^^^^^^^^^^^


"p2=p1*2"
"p22=p21*2"
"d11=30m"
"d12=20u"
"d13=4u"
"d26=1s/(cnst4*4)"


"d0=3u"
"d10=3u"

"in0=inf1/2"
"in10=inf2/2"


"DELTA1=d13+p16+d16+4u"


"DELTA=p16+d16+larger(p2,p14)+d10*2"
"DELTA2=larger(p14,p22)+d0*2"





"TAU=d8-p16-d16"


aqseq 321


;1 ze
;  d11 pl16:f3
;2 d11 do:f3
;3 d12
;********Added for NUS*******
# 1 "mc_line 88 file /opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcf3gpsi3d.nus dc-measurement inserted automatically"
    dccorr
# 88 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcf3gpsi3d.nus"
1 ze
	d11 pl16:f3
2	d11 do:f3
	short*2
3	short*5
4	1u
if "vd < 2u" goto 100
	short*6
5	short
6	d11
;^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  d1 
  (p1 ph8)
  DELTA2 pl3:f3
  (p2 ph9)
  d0


  (center (p14:sp3 ph1):f2 (p22 ph1):f3 )




  d0
  (p1 ph10)
  TAU setnmr3|0 setnmr0|34|32|33 ctrlgrad 0
  p16:gp1
  d16
  (p1 ph11)
  d26
  (center (p2 ph1) (p22 ph6):f3 )
  d26
  p28 ph1
  d13
  (p1 ph2) 
  3u
  p16:gp2
  d16
  (p21 ph3):f3
  d10 


  (center (p2 ph7) (p14:sp3 ph1):f2 )




  d10
  p16:gp3*EA
  d16
  (p22 ph4):f3
  DELTA
  (center (p1 ph1) (p21 ph4):f3 )
  d24
  (center (p2 ph1) (p22 ph1):f3 )
  d24
  (center (p1 ph2) (p21 ph5):f3 )
  d26
  (center (p2 ph1) (p22 ph1):f3 )
  d26
  (p1 ph1)
  DELTA1
  (p2 ph1)
  d13
  p16:gp4
  d16 pl16:f3
  4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7
  go=2 ph31 cpd3:f3
;********Added for NUS*******
	d11 do:f3 wr #0 if #0 zd
;----------------------------
100     short igrad EA
	short ip5*2
lo to 3 times 2

	short id10
	short ip3*2
	short ip6*2
	short ip31*2
	short ivd
lo to 4 times l2

	short rd10
	short rp3
	short rp6
	short rp31
	short ip8
	short ip9
lo to 5 times 2

	short id0
lo to 6 times l3
1m
;^^^^^^^^^^^^^^^^^^^^^^^^^^^^
;  d11 do:f3 mc #0 to 2 
;     F1PH(rd10 & rp3 & rp6 & rp31 & ip8 & ip9, id0) 
;     F2EA(igrad EA & ip5*2, id10 & ip3*2 & ip6*2 & ip31*2)
exit
   

ph1=0 
ph2=1
ph3=0 2
ph4=0 0 2 2
ph5=1 1 3 3
ph6=0
ph7=0 0 2 2
ph8=0 0 0 0 2 2 2 2
ph9=1 1 1 1 3 3 3 3
ph10=0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
     2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
ph11=0 0 0 0 0 0 0 0 2 2 2 2 2 2 2 2
ph31=0 2 2 0 2 0 0 2 2 0 0 2 0 2 2 0
     2 0 0 2 0 2 2 0 0 2 2 0 2 0 0 2


;pl1 : f1 channel - power level for pulse (default)
;pl3 : f3 channel - power level for pulse (default)
;pl16: f3 channel - power level for CPD/BB decoupling
;sp3: f2 channel - shaped pulse 180 degree (adiabatic)
;p1 : f1 channel -  90 degree high power pulse
;p2 : f1 channel - 180 degree high power pulse
;p14: f2 channel - 180 degree shaped pulse for inversion (adiabatic)
;p16: homospoil/gradient pulse                         [1 msec]
;p21: f3 channel -  90 degree high power pulse
;p22: f3 channel - 180 degree high power pulse
;p28: f1 channel - trim pulse                          [1 msec]
;d0 : incremented delay (F1 in 3D)                     [3 usec]
;d1 : relaxation delay; 1-5 * T1
;d8 : mixing time
;d10: incremented delay (F2 in 3D)                     [3 usec]
;d11: delay for disk I/O                               [30 msec]
;d12: delay for power switching                        [20 usec]
;d13: short delay                                      [4 usec]
;d16: delay for homospoil/gradient recovery
;d24: 1/(4J)YH for YH
;     1/(8J)YH for all multiplicities
;d26: 1/(4J(YH))
;cnst4: = J(YH)
;inf1: 1/SW(H) = 2 * DW(H)
;inf2: 1/SW(X) = 2 * DW(X)
;in0: 1/(2 * SW(H)) = DW(H)
;nd0: 2
;in10: 1/(2 * SW(X)) = DW(X)
;nd10: 2
;NS: 8 * n
;DS: >= 16
;td1: number of experiments in F1
;td2: number of experiments in F2
;FnMODE: States-TPPI (or TPPI) in F1
;FnMODE: echo-antiecho in F2
;cpd3: decoupling according to sequence defined by cpdprg3
;pcpd3: f3 channel - 90 degree pulse for decoupling sequence


;use gradient ratio:    gp 1 : gp 2 : gp 3 : gp 4
;                         30 :   50 :   80 :  8.1      for N-15
;                         30 :   50 :   80 : 20.1      for C-13

;for z-only gradients:
;gpz1: 30%
;gpz2: 50%
;gpz3: 80%
;gpz4: 8.1% for N-15, 20.1% for C-13

;use gradient files:   
;gpnam1: SINE.100
;gpnam2: SINE.100
;gpnam3: SINE.100
;gpnam4: SINE.100


                                          ;preprocessor-flags-start
;1: for C-13 and N-15 labeled samples start experiment with 
;             option -DLABEL_CN (eda: ZGOPTNS)
                                          ;preprocessor-flags-end



;$Id: pulseprogram,v 1.1 2021/02/11 16:17:33 bmrbsvc Exp $