data_17095 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 17095 _Entry.Title ; Identification by NMR of the Binding Surface for the Histidine-Containing Phosphocarrier Protein HPr on the N-Terminal Domain of Enzyme of the Escherichia coli Phophotransferase System ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-08-02 _Entry.Accession_date 2010-08-02 _Entry.Last_release_date 2010-11-10 _Entry.Original_release_date 2010-11-10 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Daniel Garrett . S. . 17095 2 Yeong-Jae Seok . . . 17095 3 Alan Peterkofsky . . . 17095 4 G. Clore . M. . 17095 5 Angela Gronenborn . M. . 17095 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID chemical_rates 1 17095 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'binding constants' 1 17095 'kinetic rates' 2 17095 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2010-11-10 2010-08-02 original author . 17095 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 17095 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 9109646 _Citation.Full_citation . _Citation.Title 'Identification by NMR of the Binding Surface for the Histidine-Containing Phosphocarrier Protein HPr on the N-Terminal Domain of Enzyme I of the Escherichia coli Phosphotransferase System' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev Biochemistry _Citation.Journal_name_full Biochemistry _Citation.Journal_volume 36 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 4393 _Citation.Page_last 4398 _Citation.Year 1997 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Daniel Garrett . S. . 17095 1 2 Yeong-Jae Seok . . . 17095 1 3 Alan Peterkofsky . . . 17095 1 4 G. Clore . M. . 17095 1 5 Angela Gronenborn . M. . 17095 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 17095 _Assembly.ID 1 _Assembly.Name 'EIN HPr complex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 EIN 1 $EIN A . yes native no no . . . 17095 1 2 HPr 2 $HPr B . yes native no no . . . 17095 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_EIN _Entity.Sf_category entity _Entity.Sf_framecode EIN _Entity.Entry_ID 17095 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name EIN _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MISGILASPGIAFGKALLLK EDEIVIDRKKISADQVDQEV ERFLSGRAKASAQLETIKTK AGETFGEEKEAIFEGHIMLL EDEELEQEIIALIKDKHMTA DAAAHEVIEGQASALEELDD EYLKERAADVRDIGKRLLRN ILGLKIIDLSAIQDEVILVA ADLTPSETAQLNLKKVLGFI TDAGGRTSHTSIMARSLELP AIVGTGSVTSQVKNDDYLIL DAVNNQVYVNPTNEVIDKMR AVQEQVASEKAELAKLKDR ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 259 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details 'Enzyme I' _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 19958 . N-terminal_Domain_of_Enzyme_I . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 2 no BMRB 25731 . entity . . . . . 99.61 573 100.00 100.00 6.45e-175 . . . . 17095 1 3 no BMRB 4106 . "N-terminal Domain of Enzyme I" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 4 no BMRB 4264 . "Enzyme I, N-terminal" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 5 no PDB 1EZA . "Amino Terminal Domain Of Enzyme I From Escherichia Coli Nmr, Restrained Regularized Mean Structure" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 6 no PDB 1EZB . "Amino Terminal Domain Of Enzyme I From Escherichia Coli, Nmr, 17 Structures" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 7 no PDB 1EZC . "Amino Terminal Domain Of Enzyme I From Escherichia Coli, Nmr, 17 Structures" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 8 no PDB 1EZD . "Amino Terminal Domain Of Enzyme I From Escherichia Coli Nmr, 16 Structures" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 9 no PDB 1ZYM . "Amino Terminal Domain Of Enzyme I From Escherichia Coli" . . . . . 99.61 258 100.00 100.00 4.13e-180 . . . . 17095 1 10 no PDB 2EZA . "Amino Terminal Domain Of Enzyme I From Escherichia Coli, Nmr, Restrained Regularized Mean Structure" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 11 no PDB 2EZB . "Amino Terminal Domain Of Enzyme I From Escherichia Coli, Nmr, 14 Structures" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 12 no PDB 2EZC . "Amino Terminal Domain Of Enzyme I From Escherichia Coli, Nmr, 14 Structures" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 13 no PDB 2HWG . "Structure Of Phosphorylated Enzyme I Of The Phosphoenolpyruvate:sugar Phosphotransferase System" . . . . . 99.23 575 98.05 98.05 9.40e-168 . . . . 17095 1 14 no PDB 2KX9 . "Solution Structure Of The Enzyme I Dimer Using Residual Dipolar Couplings And Small Angle X-Ray Scattering" . . . . . 99.61 573 100.00 100.00 6.45e-175 . . . . 17095 1 15 no PDB 2L5H . "Solution Structure Of The H189q Mutant Of The Enzyme I Dimer Using Residual Dipolar Couplings And Small Angle X-Ray Scattering" . . . . . 99.61 573 99.61 99.61 1.08e-173 . . . . 17095 1 16 no PDB 2MP0 . "Protein Phosphorylation Upon A Fleeting Encounter" . . . . . 99.61 258 100.00 100.00 4.13e-180 . . . . 17095 1 17 no PDB 2N5T . "Ensemble Solution Structure Of The Phosphoenolpyruvate-enzyme I Complex From The Bacterial Hosphotransferase System" . . . . . 99.61 575 100.00 100.00 7.04e-175 . . . . 17095 1 18 no PDB 2XDF . "Solution Structure Of The Enzyme I Dimer Complexed With Hpr Using Residual Dipolar Couplings And Small Angle X-Ray Scattering" . . . . . 99.61 573 100.00 100.00 6.45e-175 . . . . 17095 1 19 no PDB 3EZA . "Complex Of The Amino Terminal Domain Of Enzyme I And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli " . . . . . 96.14 249 100.00 100.00 2.96e-174 . . . . 17095 1 20 no PDB 3EZB . "Complex Of The Amino Terminal Domain Of Enzyme I And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli" . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 21 no PDB 3EZE . "Complex Of The Amino Terminal Domain Of Enzyme I And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli " . . . . . 100.00 259 100.00 100.00 0.00e+00 . . . . 17095 1 22 no DBJ BAA16290 . "PEP-protein phosphotransferase of PTS system [Escherichia coli str. K12 substr. W3110]" . . . . . 99.61 575 100.00 100.00 7.04e-175 . . . . 17095 1 23 no DBJ BAB36711 . "PEP-protein phosphotransferase system enzyme I [Escherichia coli O157:H7 str. Sakai]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 24 no DBJ BAG78231 . "PTS system enzyme I [Escherichia coli SE11]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 25 no DBJ BAI26669 . "PEP-protein phosphotransferase PtsI [Escherichia coli O26:H11 str. 11368]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 26 no DBJ BAI31701 . "PEP-protein phosphotransferase PtsI [Escherichia coli O103:H2 str. 12009]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 27 no EMBL CAP76888 . "Phosphoenolpyruvate-protein phosphotransferase [Escherichia coli LF82]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 28 no EMBL CAQ32794 . "PTS enzyme I, subunit of EIIAsc, EIIBgl, EIIChb, CmtAB mannitol PTS permease, enzyme II [glc], fructose PTS transporter, EIIABC" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 29 no EMBL CAQ88296 . "PEP-protein phosphotransferase of PTS system (enzyme I) [Escherichia fergusonii ATCC 35469]" . . . . . 99.61 575 98.06 99.22 1.65e-171 . . . . 17095 1 30 no EMBL CAQ99314 . "PEP-protein phosphotransferase of PTS system (enzyme I) [Escherichia coli IAI1]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 31 no EMBL CAR03878 . "PEP-protein phosphotransferase of PTS system (enzyme I) [Escherichia coli S88]" . . . . . 99.61 575 100.00 100.00 5.25e-175 . . . . 17095 1 32 no GB AAA24385 . "enzyme I [Escherichia coli]" . . . . . 99.61 575 100.00 100.00 7.04e-175 . . . . 17095 1 33 no GB AAA24441 . "enzyme I [Escherichia coli]" . . . . . 99.61 575 100.00 100.00 7.04e-175 . . . . 17095 1 34 no GB AAC75469 . "PEP-protein phosphotransferase of PTS system (enzyme I) [Escherichia coli str. K-12 substr. MG1655]" . . . . . 99.61 575 100.00 100.00 7.04e-175 . . . . 17095 1 35 no GB AAG57535 . "PEP-protein phosphotransferase system enzyme I [Escherichia coli O157:H7 str. EDL933]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 36 no GB AAN43978 . "PEP-protein phosphotransferase system enzyme I [Shigella flexneri 2a str. 301]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 37 no REF NP_311315 . "phosphoenolpyruvate-protein phosphotransferase [Escherichia coli O157:H7 str. Sakai]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 38 no REF NP_416911 . "PEP-protein phosphotransferase of PTS system (enzyme I) [Escherichia coli str. K-12 substr. MG1655]" . . . . . 99.61 575 100.00 100.00 7.04e-175 . . . . 17095 1 39 no REF NP_708271 . "phosphoenolpyruvate-protein phosphotransferase [Shigella flexneri 2a str. 301]" . . . . . 99.61 575 100.00 100.00 5.72e-175 . . . . 17095 1 40 no REF WP_000112669 . "phosphoenolpyruvate--protein phosphotransferase, partial [Escherichia coli]" . . . . . 85.71 539 100.00 100.00 2.00e-148 . . . . 17095 1 41 no REF WP_000328837 . "phosphoenolpyruvate--protein phosphotransferase, partial [Escherichia coli]" . . . . . 52.90 454 100.00 100.00 2.57e-85 . . . . 17095 1 42 no SP P08839 . "RecName: Full=Phosphoenolpyruvate-protein phosphotransferase; AltName: Full=Phosphotransferase system, enzyme I" . . . . . 99.61 575 100.00 100.00 7.04e-175 . . . . 17095 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 17095 1 2 . ILE . 17095 1 3 . SER . 17095 1 4 . GLY . 17095 1 5 . ILE . 17095 1 6 . LEU . 17095 1 7 . ALA . 17095 1 8 . SER . 17095 1 9 . PRO . 17095 1 10 . GLY . 17095 1 11 . ILE . 17095 1 12 . ALA . 17095 1 13 . PHE . 17095 1 14 . GLY . 17095 1 15 . LYS . 17095 1 16 . ALA . 17095 1 17 . LEU . 17095 1 18 . LEU . 17095 1 19 . LEU . 17095 1 20 . LYS . 17095 1 21 . GLU . 17095 1 22 . ASP . 17095 1 23 . GLU . 17095 1 24 . ILE . 17095 1 25 . VAL . 17095 1 26 . ILE . 17095 1 27 . ASP . 17095 1 28 . ARG . 17095 1 29 . LYS . 17095 1 30 . LYS . 17095 1 31 . ILE . 17095 1 32 . SER . 17095 1 33 . ALA . 17095 1 34 . ASP . 17095 1 35 . GLN . 17095 1 36 . VAL . 17095 1 37 . ASP . 17095 1 38 . GLN . 17095 1 39 . GLU . 17095 1 40 . VAL . 17095 1 41 . GLU . 17095 1 42 . ARG . 17095 1 43 . PHE . 17095 1 44 . LEU . 17095 1 45 . SER . 17095 1 46 . GLY . 17095 1 47 . ARG . 17095 1 48 . ALA . 17095 1 49 . LYS . 17095 1 50 . ALA . 17095 1 51 . SER . 17095 1 52 . ALA . 17095 1 53 . GLN . 17095 1 54 . LEU . 17095 1 55 . GLU . 17095 1 56 . THR . 17095 1 57 . ILE . 17095 1 58 . LYS . 17095 1 59 . THR . 17095 1 60 . LYS . 17095 1 61 . ALA . 17095 1 62 . GLY . 17095 1 63 . GLU . 17095 1 64 . THR . 17095 1 65 . PHE . 17095 1 66 . GLY . 17095 1 67 . GLU . 17095 1 68 . GLU . 17095 1 69 . LYS . 17095 1 70 . GLU . 17095 1 71 . ALA . 17095 1 72 . ILE . 17095 1 73 . PHE . 17095 1 74 . GLU . 17095 1 75 . GLY . 17095 1 76 . HIS . 17095 1 77 . ILE . 17095 1 78 . MET . 17095 1 79 . LEU . 17095 1 80 . LEU . 17095 1 81 . GLU . 17095 1 82 . ASP . 17095 1 83 . GLU . 17095 1 84 . GLU . 17095 1 85 . LEU . 17095 1 86 . GLU . 17095 1 87 . GLN . 17095 1 88 . GLU . 17095 1 89 . ILE . 17095 1 90 . ILE . 17095 1 91 . ALA . 17095 1 92 . LEU . 17095 1 93 . ILE . 17095 1 94 . LYS . 17095 1 95 . ASP . 17095 1 96 . LYS . 17095 1 97 . HIS . 17095 1 98 . MET . 17095 1 99 . THR . 17095 1 100 . ALA . 17095 1 101 . ASP . 17095 1 102 . ALA . 17095 1 103 . ALA . 17095 1 104 . ALA . 17095 1 105 . HIS . 17095 1 106 . GLU . 17095 1 107 . VAL . 17095 1 108 . ILE . 17095 1 109 . GLU . 17095 1 110 . GLY . 17095 1 111 . GLN . 17095 1 112 . ALA . 17095 1 113 . SER . 17095 1 114 . ALA . 17095 1 115 . LEU . 17095 1 116 . GLU . 17095 1 117 . GLU . 17095 1 118 . LEU . 17095 1 119 . ASP . 17095 1 120 . ASP . 17095 1 121 . GLU . 17095 1 122 . TYR . 17095 1 123 . LEU . 17095 1 124 . LYS . 17095 1 125 . GLU . 17095 1 126 . ARG . 17095 1 127 . ALA . 17095 1 128 . ALA . 17095 1 129 . ASP . 17095 1 130 . VAL . 17095 1 131 . ARG . 17095 1 132 . ASP . 17095 1 133 . ILE . 17095 1 134 . GLY . 17095 1 135 . LYS . 17095 1 136 . ARG . 17095 1 137 . LEU . 17095 1 138 . LEU . 17095 1 139 . ARG . 17095 1 140 . ASN . 17095 1 141 . ILE . 17095 1 142 . LEU . 17095 1 143 . GLY . 17095 1 144 . LEU . 17095 1 145 . LYS . 17095 1 146 . ILE . 17095 1 147 . ILE . 17095 1 148 . ASP . 17095 1 149 . LEU . 17095 1 150 . SER . 17095 1 151 . ALA . 17095 1 152 . ILE . 17095 1 153 . GLN . 17095 1 154 . ASP . 17095 1 155 . GLU . 17095 1 156 . VAL . 17095 1 157 . ILE . 17095 1 158 . LEU . 17095 1 159 . VAL . 17095 1 160 . ALA . 17095 1 161 . ALA . 17095 1 162 . ASP . 17095 1 163 . LEU . 17095 1 164 . THR . 17095 1 165 . PRO . 17095 1 166 . SER . 17095 1 167 . GLU . 17095 1 168 . THR . 17095 1 169 . ALA . 17095 1 170 . GLN . 17095 1 171 . LEU . 17095 1 172 . ASN . 17095 1 173 . LEU . 17095 1 174 . LYS . 17095 1 175 . LYS . 17095 1 176 . VAL . 17095 1 177 . LEU . 17095 1 178 . GLY . 17095 1 179 . PHE . 17095 1 180 . ILE . 17095 1 181 . THR . 17095 1 182 . ASP . 17095 1 183 . ALA . 17095 1 184 . GLY . 17095 1 185 . GLY . 17095 1 186 . ARG . 17095 1 187 . THR . 17095 1 188 . SER . 17095 1 189 . HIS . 17095 1 190 . THR . 17095 1 191 . SER . 17095 1 192 . ILE . 17095 1 193 . MET . 17095 1 194 . ALA . 17095 1 195 . ARG . 17095 1 196 . SER . 17095 1 197 . LEU . 17095 1 198 . GLU . 17095 1 199 . LEU . 17095 1 200 . PRO . 17095 1 201 . ALA . 17095 1 202 . ILE . 17095 1 203 . VAL . 17095 1 204 . GLY . 17095 1 205 . THR . 17095 1 206 . GLY . 17095 1 207 . SER . 17095 1 208 . VAL . 17095 1 209 . THR . 17095 1 210 . SER . 17095 1 211 . GLN . 17095 1 212 . VAL . 17095 1 213 . LYS . 17095 1 214 . ASN . 17095 1 215 . ASP . 17095 1 216 . ASP . 17095 1 217 . TYR . 17095 1 218 . LEU . 17095 1 219 . ILE . 17095 1 220 . LEU . 17095 1 221 . ASP . 17095 1 222 . ALA . 17095 1 223 . VAL . 17095 1 224 . ASN . 17095 1 225 . ASN . 17095 1 226 . GLN . 17095 1 227 . VAL . 17095 1 228 . TYR . 17095 1 229 . VAL . 17095 1 230 . ASN . 17095 1 231 . PRO . 17095 1 232 . THR . 17095 1 233 . ASN . 17095 1 234 . GLU . 17095 1 235 . VAL . 17095 1 236 . ILE . 17095 1 237 . ASP . 17095 1 238 . LYS . 17095 1 239 . MET . 17095 1 240 . ARG . 17095 1 241 . ALA . 17095 1 242 . VAL . 17095 1 243 . GLN . 17095 1 244 . GLU . 17095 1 245 . GLN . 17095 1 246 . VAL . 17095 1 247 . ALA . 17095 1 248 . SER . 17095 1 249 . GLU . 17095 1 250 . LYS . 17095 1 251 . ALA . 17095 1 252 . GLU . 17095 1 253 . LEU . 17095 1 254 . ALA . 17095 1 255 . LYS . 17095 1 256 . LEU . 17095 1 257 . LYS . 17095 1 258 . ASP . 17095 1 259 . ARG . 17095 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 17095 1 . ILE 2 2 17095 1 . SER 3 3 17095 1 . GLY 4 4 17095 1 . ILE 5 5 17095 1 . LEU 6 6 17095 1 . ALA 7 7 17095 1 . SER 8 8 17095 1 . PRO 9 9 17095 1 . GLY 10 10 17095 1 . ILE 11 11 17095 1 . ALA 12 12 17095 1 . PHE 13 13 17095 1 . GLY 14 14 17095 1 . LYS 15 15 17095 1 . ALA 16 16 17095 1 . LEU 17 17 17095 1 . LEU 18 18 17095 1 . LEU 19 19 17095 1 . LYS 20 20 17095 1 . GLU 21 21 17095 1 . ASP 22 22 17095 1 . GLU 23 23 17095 1 . ILE 24 24 17095 1 . VAL 25 25 17095 1 . ILE 26 26 17095 1 . ASP 27 27 17095 1 . ARG 28 28 17095 1 . LYS 29 29 17095 1 . LYS 30 30 17095 1 . ILE 31 31 17095 1 . SER 32 32 17095 1 . ALA 33 33 17095 1 . ASP 34 34 17095 1 . GLN 35 35 17095 1 . VAL 36 36 17095 1 . ASP 37 37 17095 1 . GLN 38 38 17095 1 . GLU 39 39 17095 1 . VAL 40 40 17095 1 . GLU 41 41 17095 1 . ARG 42 42 17095 1 . PHE 43 43 17095 1 . LEU 44 44 17095 1 . SER 45 45 17095 1 . GLY 46 46 17095 1 . ARG 47 47 17095 1 . ALA 48 48 17095 1 . LYS 49 49 17095 1 . ALA 50 50 17095 1 . SER 51 51 17095 1 . ALA 52 52 17095 1 . GLN 53 53 17095 1 . LEU 54 54 17095 1 . GLU 55 55 17095 1 . THR 56 56 17095 1 . ILE 57 57 17095 1 . LYS 58 58 17095 1 . THR 59 59 17095 1 . LYS 60 60 17095 1 . ALA 61 61 17095 1 . GLY 62 62 17095 1 . GLU 63 63 17095 1 . THR 64 64 17095 1 . PHE 65 65 17095 1 . GLY 66 66 17095 1 . GLU 67 67 17095 1 . GLU 68 68 17095 1 . LYS 69 69 17095 1 . GLU 70 70 17095 1 . ALA 71 71 17095 1 . ILE 72 72 17095 1 . PHE 73 73 17095 1 . GLU 74 74 17095 1 . GLY 75 75 17095 1 . HIS 76 76 17095 1 . ILE 77 77 17095 1 . MET 78 78 17095 1 . LEU 79 79 17095 1 . LEU 80 80 17095 1 . GLU 81 81 17095 1 . ASP 82 82 17095 1 . GLU 83 83 17095 1 . GLU 84 84 17095 1 . LEU 85 85 17095 1 . GLU 86 86 17095 1 . GLN 87 87 17095 1 . GLU 88 88 17095 1 . ILE 89 89 17095 1 . ILE 90 90 17095 1 . ALA 91 91 17095 1 . LEU 92 92 17095 1 . ILE 93 93 17095 1 . LYS 94 94 17095 1 . ASP 95 95 17095 1 . LYS 96 96 17095 1 . HIS 97 97 17095 1 . MET 98 98 17095 1 . THR 99 99 17095 1 . ALA 100 100 17095 1 . ASP 101 101 17095 1 . ALA 102 102 17095 1 . ALA 103 103 17095 1 . ALA 104 104 17095 1 . HIS 105 105 17095 1 . GLU 106 106 17095 1 . VAL 107 107 17095 1 . ILE 108 108 17095 1 . GLU 109 109 17095 1 . GLY 110 110 17095 1 . GLN 111 111 17095 1 . ALA 112 112 17095 1 . SER 113 113 17095 1 . ALA 114 114 17095 1 . LEU 115 115 17095 1 . GLU 116 116 17095 1 . GLU 117 117 17095 1 . LEU 118 118 17095 1 . ASP 119 119 17095 1 . ASP 120 120 17095 1 . GLU 121 121 17095 1 . TYR 122 122 17095 1 . LEU 123 123 17095 1 . LYS 124 124 17095 1 . GLU 125 125 17095 1 . ARG 126 126 17095 1 . ALA 127 127 17095 1 . ALA 128 128 17095 1 . ASP 129 129 17095 1 . VAL 130 130 17095 1 . ARG 131 131 17095 1 . ASP 132 132 17095 1 . ILE 133 133 17095 1 . GLY 134 134 17095 1 . LYS 135 135 17095 1 . ARG 136 136 17095 1 . LEU 137 137 17095 1 . LEU 138 138 17095 1 . ARG 139 139 17095 1 . ASN 140 140 17095 1 . ILE 141 141 17095 1 . LEU 142 142 17095 1 . GLY 143 143 17095 1 . LEU 144 144 17095 1 . LYS 145 145 17095 1 . ILE 146 146 17095 1 . ILE 147 147 17095 1 . ASP 148 148 17095 1 . LEU 149 149 17095 1 . SER 150 150 17095 1 . ALA 151 151 17095 1 . ILE 152 152 17095 1 . GLN 153 153 17095 1 . ASP 154 154 17095 1 . GLU 155 155 17095 1 . VAL 156 156 17095 1 . ILE 157 157 17095 1 . LEU 158 158 17095 1 . VAL 159 159 17095 1 . ALA 160 160 17095 1 . ALA 161 161 17095 1 . ASP 162 162 17095 1 . LEU 163 163 17095 1 . THR 164 164 17095 1 . PRO 165 165 17095 1 . SER 166 166 17095 1 . GLU 167 167 17095 1 . THR 168 168 17095 1 . ALA 169 169 17095 1 . GLN 170 170 17095 1 . LEU 171 171 17095 1 . ASN 172 172 17095 1 . LEU 173 173 17095 1 . LYS 174 174 17095 1 . LYS 175 175 17095 1 . VAL 176 176 17095 1 . LEU 177 177 17095 1 . GLY 178 178 17095 1 . PHE 179 179 17095 1 . ILE 180 180 17095 1 . THR 181 181 17095 1 . ASP 182 182 17095 1 . ALA 183 183 17095 1 . GLY 184 184 17095 1 . GLY 185 185 17095 1 . ARG 186 186 17095 1 . THR 187 187 17095 1 . SER 188 188 17095 1 . HIS 189 189 17095 1 . THR 190 190 17095 1 . SER 191 191 17095 1 . ILE 192 192 17095 1 . MET 193 193 17095 1 . ALA 194 194 17095 1 . ARG 195 195 17095 1 . SER 196 196 17095 1 . LEU 197 197 17095 1 . GLU 198 198 17095 1 . LEU 199 199 17095 1 . PRO 200 200 17095 1 . ALA 201 201 17095 1 . ILE 202 202 17095 1 . VAL 203 203 17095 1 . GLY 204 204 17095 1 . THR 205 205 17095 1 . GLY 206 206 17095 1 . SER 207 207 17095 1 . VAL 208 208 17095 1 . THR 209 209 17095 1 . SER 210 210 17095 1 . GLN 211 211 17095 1 . VAL 212 212 17095 1 . LYS 213 213 17095 1 . ASN 214 214 17095 1 . ASP 215 215 17095 1 . ASP 216 216 17095 1 . TYR 217 217 17095 1 . LEU 218 218 17095 1 . ILE 219 219 17095 1 . LEU 220 220 17095 1 . ASP 221 221 17095 1 . ALA 222 222 17095 1 . VAL 223 223 17095 1 . ASN 224 224 17095 1 . ASN 225 225 17095 1 . GLN 226 226 17095 1 . VAL 227 227 17095 1 . TYR 228 228 17095 1 . VAL 229 229 17095 1 . ASN 230 230 17095 1 . PRO 231 231 17095 1 . THR 232 232 17095 1 . ASN 233 233 17095 1 . GLU 234 234 17095 1 . VAL 235 235 17095 1 . ILE 236 236 17095 1 . ASP 237 237 17095 1 . LYS 238 238 17095 1 . MET 239 239 17095 1 . ARG 240 240 17095 1 . ALA 241 241 17095 1 . VAL 242 242 17095 1 . GLN 243 243 17095 1 . GLU 244 244 17095 1 . GLN 245 245 17095 1 . VAL 246 246 17095 1 . ALA 247 247 17095 1 . SER 248 248 17095 1 . GLU 249 249 17095 1 . LYS 250 250 17095 1 . ALA 251 251 17095 1 . GLU 252 252 17095 1 . LEU 253 253 17095 1 . ALA 254 254 17095 1 . LYS 255 255 17095 1 . LEU 256 256 17095 1 . LYS 257 257 17095 1 . ASP 258 258 17095 1 . ARG 259 259 17095 1 stop_ save_ save_HPr _Entity.Sf_category entity _Entity.Sf_framecode HPr _Entity.Entry_ID 17095 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name HPr _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MFQQEVTITAPNGLHTRPAA QFVKEAKGFTSEITVTSNGK SASAKSLFKLQTLGLTQGTV VTISAEGEDEQKAVEHLVKL MAELE ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 85 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details 'Histidine containing phophocarrier protein' _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-30 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 17274 . HPr . . . . . 100.00 85 97.65 97.65 1.81e-49 . . . . 17095 2 2 no BMRB 18379 . entity_2 . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 3 no BMRB 2060 . "phosphocarrier protein HPr" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 4 no BMRB 2371 . "phosphocarrier protein HPr" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 5 no BMRB 29 . "phosphocarrier protein HPr" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 6 no BMRB 4264 . HPr . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 7 no PDB 1CM2 . "Structure Of His15asp Hpr After Hydrolysis Of Ringed Species." . . . . . 100.00 85 98.82 98.82 9.25e-51 . . . . 17095 2 8 no PDB 1CM3 . "His15asp Hpr From E. Coli" . . . . . 100.00 85 98.82 98.82 9.25e-51 . . . . 17095 2 9 no PDB 1GGR . "Complex Of Enzyme Iiaglc And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli Nmr, Restrained Regulari" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 10 no PDB 1HDN . "The High-Resolution Structure Of The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli Determined By Restra" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 11 no PDB 1J6T . "Complex Of Enzyme Iiamtl And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli Nmr, Restrained Regulari" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 12 no PDB 1OPD . "Histidine-Containing Protein (Hpr), Mutant With Ser 46 Replaced By Asp (S46d)" . . . . . 100.00 85 97.65 98.82 9.77e-51 . . . . 17095 2 13 no PDB 1PFH . "The Phosphorylated Form Of The Histidine-Containing Phosphocarrier Protein Hpr" . . . . . 100.00 85 98.82 98.82 1.27e-50 . . . . 17095 2 14 no PDB 1POH . "The 2.0 Angstroms Resolution Structure Of Escherichia Coli Histidine-containing Phosphocarrier Protein Hpr: A Redetermination" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 15 no PDB 1VRC . "Complex Of Enzyme Iiamannose And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli Nmr, Restrained Regu" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 16 no PDB 2JEL . "Jel42 FabHPR COMPLEX" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 17 no PDB 2LRK . "Solution Structures Of The Iia(Chitobiose)-Hpr Complex Of The N,N'- Diacetylchitobiose" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 18 no PDB 2LRL . "Solution Structures Of The Iia(Chitobiose)-Hpr Complex Of The N,N'- Diacetylchitobiose Branch Of The Escherichia Coli Phosphotr" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 19 no PDB 2XDF . "Solution Structure Of The Enzyme I Dimer Complexed With Hpr Using Residual Dipolar Couplings And Small Angle X-Ray Scattering" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 20 no PDB 3CCD . "1.0 A Structure Of Post-Succinimide His15asp Hpr" . . . . . 100.00 85 97.65 98.82 3.77e-50 . . . . 17095 2 21 no PDB 3EZA . "Complex Of The Amino Terminal Domain Of Enzyme I And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli " . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 22 no PDB 3EZB . "Complex Of The Amino Terminal Domain Of Enzyme I And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 23 no PDB 3EZE . "Complex Of The Amino Terminal Domain Of Enzyme I And The Histidine-Containing Phosphocarrier Protein Hpr From Escherichia Coli " . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 24 no DBJ BAA16289 . "phosphohistidinoprotein-hexose phosphotransferase component of PTS system (Hpr) [Escherichia coli str. K-12 substr. W3110]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 25 no DBJ BAB36710 . "PTS system protein HPr [Escherichia coli O157:H7 str. Sakai]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 26 no DBJ BAB92987 . "HPr of PTS system [Serratia marcescens]" . . . . . 100.00 85 98.82 100.00 1.29e-51 . . . . 17095 2 27 no DBJ BAG78230 . "phosphocarrier protein HPr [Escherichia coli SE11]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 28 no DBJ BAH64556 . "PTS family phosphohistidinoprotein-hexose phosphotransferase [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]" . . . . . 100.00 85 98.82 100.00 1.47e-51 . . . . 17095 2 29 no EMBL CAA32865 . "unnamed protein product [Salmonella enterica subsp. enterica serovar Typhimurium]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 30 no EMBL CAA35818 . "unnamed protein product [Klebsiella pneumoniae]" . . . . . 100.00 85 98.82 100.00 1.47e-51 . . . . 17095 2 31 no EMBL CAD07663 . "phosphocarrier protein HPr [Salmonella enterica subsp. enterica serovar Typhi str. CT18]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 32 no EMBL CAG73805 . "PTS system phosphocarrier protein [Pectobacterium atrosepticum SCRI1043]" . . . . . 100.00 85 97.65 100.00 4.36e-51 . . . . 17095 2 33 no EMBL CAH21953 . "PTS system, phosphocarrier protein [Yersinia pseudotuberculosis IP 32953]" . . . . . 100.00 85 97.65 100.00 6.74e-51 . . . . 17095 2 34 no GB AAA23655 . "ptsH protein [Escherichia coli]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 35 no GB AAA24384 . "HPr protein (EC 2.7.1.68) [Escherichia coli]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 36 no GB AAA24438 . "histidine-containing protein [Escherichia coli]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 37 no GB AAA24440 . "HPr [Escherichia coli]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 38 no GB AAA27052 . "ptsH protein [Salmonella enterica subsp. enterica serovar Typhimurium]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 39 no PIR AE0810 . "phosphocarrier protein HPr [imported] - Salmonella enterica subsp. enterica serovar Typhi (strain CT18)" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 40 no PRF 1107231B . "protein,His containing" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 41 no REF NP_288977 . "PTS system phosphohistidinoprotein-hexose phosphotransferase Hpr [Escherichia coli O157:H7 str. EDL933]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 42 no REF NP_311314 . "PTS system phosphohistidinoprotein-hexose phosphotransferase Hpr [Escherichia coli O157:H7 str. Sakai]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 43 no REF NP_416910 . "phosphohistidinoprotein-hexose phosphotransferase component of PTS system (Hpr) [Escherichia coli str. K-12 substr. MG1655]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 44 no REF NP_456968 . "phosphocarrier protein HPr [Salmonella enterica subsp. enterica serovar Typhi str. CT18]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 45 no REF NP_461366 . "PTS system phosphohistidinoprotein-hexose phosphotransferase Hpr [Salmonella enterica subsp. enterica serovar Typhimurium str. " . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 46 no SP P0AA04 . "RecName: Full=Phosphocarrier protein HPr; AltName: Full=Histidine-containing protein [Escherichia coli K-12]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 47 no SP P0AA05 . "RecName: Full=Phosphocarrier protein HPr; AltName: Full=Histidine-containing protein [Escherichia coli CFT073]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 48 no SP P0AA06 . "RecName: Full=Phosphocarrier protein HPr; AltName: Full=Histidine-containing protein [Escherichia coli O157:H7]" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 49 no SP P0AA07 . "RecName: Full=Phosphocarrier protein HPr; AltName: Full=Histidine-containing protein [Salmonella enterica subsp. enterica serov" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 50 no SP P0AA08 . "RecName: Full=Phosphocarrier protein HPr; AltName: Full=Histidine-containing protein [Salmonella enterica subsp. enterica serov" . . . . . 100.00 85 100.00 100.00 5.05e-52 . . . . 17095 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 17095 2 2 . PHE . 17095 2 3 . GLN . 17095 2 4 . GLN . 17095 2 5 . GLU . 17095 2 6 . VAL . 17095 2 7 . THR . 17095 2 8 . ILE . 17095 2 9 . THR . 17095 2 10 . ALA . 17095 2 11 . PRO . 17095 2 12 . ASN . 17095 2 13 . GLY . 17095 2 14 . LEU . 17095 2 15 . HIS . 17095 2 16 . THR . 17095 2 17 . ARG . 17095 2 18 . PRO . 17095 2 19 . ALA . 17095 2 20 . ALA . 17095 2 21 . GLN . 17095 2 22 . PHE . 17095 2 23 . VAL . 17095 2 24 . LYS . 17095 2 25 . GLU . 17095 2 26 . ALA . 17095 2 27 . LYS . 17095 2 28 . GLY . 17095 2 29 . PHE . 17095 2 30 . THR . 17095 2 31 . SER . 17095 2 32 . GLU . 17095 2 33 . ILE . 17095 2 34 . THR . 17095 2 35 . VAL . 17095 2 36 . THR . 17095 2 37 . SER . 17095 2 38 . ASN . 17095 2 39 . GLY . 17095 2 40 . LYS . 17095 2 41 . SER . 17095 2 42 . ALA . 17095 2 43 . SER . 17095 2 44 . ALA . 17095 2 45 . LYS . 17095 2 46 . SER . 17095 2 47 . LEU . 17095 2 48 . PHE . 17095 2 49 . LYS . 17095 2 50 . LEU . 17095 2 51 . GLN . 17095 2 52 . THR . 17095 2 53 . LEU . 17095 2 54 . GLY . 17095 2 55 . LEU . 17095 2 56 . THR . 17095 2 57 . GLN . 17095 2 58 . GLY . 17095 2 59 . THR . 17095 2 60 . VAL . 17095 2 61 . VAL . 17095 2 62 . THR . 17095 2 63 . ILE . 17095 2 64 . SER . 17095 2 65 . ALA . 17095 2 66 . GLU . 17095 2 67 . GLY . 17095 2 68 . GLU . 17095 2 69 . ASP . 17095 2 70 . GLU . 17095 2 71 . GLN . 17095 2 72 . LYS . 17095 2 73 . ALA . 17095 2 74 . VAL . 17095 2 75 . GLU . 17095 2 76 . HIS . 17095 2 77 . LEU . 17095 2 78 . VAL . 17095 2 79 . LYS . 17095 2 80 . LEU . 17095 2 81 . MET . 17095 2 82 . ALA . 17095 2 83 . GLU . 17095 2 84 . LEU . 17095 2 85 . GLU . 17095 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 17095 2 . PHE 2 2 17095 2 . GLN 3 3 17095 2 . GLN 4 4 17095 2 . GLU 5 5 17095 2 . VAL 6 6 17095 2 . THR 7 7 17095 2 . ILE 8 8 17095 2 . THR 9 9 17095 2 . ALA 10 10 17095 2 . PRO 11 11 17095 2 . ASN 12 12 17095 2 . GLY 13 13 17095 2 . LEU 14 14 17095 2 . HIS 15 15 17095 2 . THR 16 16 17095 2 . ARG 17 17 17095 2 . PRO 18 18 17095 2 . ALA 19 19 17095 2 . ALA 20 20 17095 2 . GLN 21 21 17095 2 . PHE 22 22 17095 2 . VAL 23 23 17095 2 . LYS 24 24 17095 2 . GLU 25 25 17095 2 . ALA 26 26 17095 2 . LYS 27 27 17095 2 . GLY 28 28 17095 2 . PHE 29 29 17095 2 . THR 30 30 17095 2 . SER 31 31 17095 2 . GLU 32 32 17095 2 . ILE 33 33 17095 2 . THR 34 34 17095 2 . VAL 35 35 17095 2 . THR 36 36 17095 2 . SER 37 37 17095 2 . ASN 38 38 17095 2 . GLY 39 39 17095 2 . LYS 40 40 17095 2 . SER 41 41 17095 2 . ALA 42 42 17095 2 . SER 43 43 17095 2 . ALA 44 44 17095 2 . LYS 45 45 17095 2 . SER 46 46 17095 2 . LEU 47 47 17095 2 . PHE 48 48 17095 2 . LYS 49 49 17095 2 . LEU 50 50 17095 2 . GLN 51 51 17095 2 . THR 52 52 17095 2 . LEU 53 53 17095 2 . GLY 54 54 17095 2 . LEU 55 55 17095 2 . THR 56 56 17095 2 . GLN 57 57 17095 2 . GLY 58 58 17095 2 . THR 59 59 17095 2 . VAL 60 60 17095 2 . VAL 61 61 17095 2 . THR 62 62 17095 2 . ILE 63 63 17095 2 . SER 64 64 17095 2 . ALA 65 65 17095 2 . GLU 66 66 17095 2 . GLY 67 67 17095 2 . GLU 68 68 17095 2 . ASP 69 69 17095 2 . GLU 70 70 17095 2 . GLN 71 71 17095 2 . LYS 72 72 17095 2 . ALA 73 73 17095 2 . VAL 74 74 17095 2 . GLU 75 75 17095 2 . HIS 76 76 17095 2 . LEU 77 77 17095 2 . VAL 78 78 17095 2 . LYS 79 79 17095 2 . LEU 80 80 17095 2 . MET 81 81 17095 2 . ALA 82 82 17095 2 . GLU 83 83 17095 2 . LEU 84 84 17095 2 . GLU 85 85 17095 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 17095 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 2 $HPr . 562 organism . 'Escherichia coli' 'E. coli' . . Bacteria . Escherichia coli GI698 . . . . . . . . . . . . . . . . . . . . 17095 1 2 1 $EIN . 562 organism . 'Escherichia coli' 'E. coli' . . Bacteria . Escherichia coli . . . . . . . . . . . . . . . . . . . . . 17095 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 17095 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $EIN . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli GI698 . . . . . . . . . . . . . . . pLP2 . . . . . . 17095 1 2 2 $HPr . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . . . . . . . . . . . . . . pRE2 . . . . . . 17095 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 17095 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 EIN [U-15N] . . 1 $EIN . . 1 . . mM . . . . 17095 1 2 HPr [U-15N] . . 2 $HPr . . 1.6 . . mM . . . . 17095 1 3 'sodium phosphate' 'natural abundance' . . . . . . 20 . . mM . . . . 17095 1 4 H2O 'natural abundance' . . . . . . 90 . . % . . . . 17095 1 5 D2O 'natural abundance' . . . . . . 10 . . % . . . . 17095 1 stop_ save_ save_sample_2 _Sample.Sf_category sample _Sample.Sf_framecode sample_2 _Sample.Entry_ID 17095 _Sample.ID 2 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 EIN [U-15N] . . 1 $EIN . . 1 . . mM . . . . 17095 2 2 'sodium phospahte' 'natural abundance' . . . . . . 20 . . mM . . . . 17095 2 3 H2O 'natural abundance' . . . . . . 90 . . % . . . . 17095 2 4 D2O 'natural abundance' . . . . . . 10 . . % . . . . 17095 2 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 17095 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 7.1 . pH 17095 1 pressure 1 . atm 17095 1 temperature 313 . K 17095 1 stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Software.Sf_category software _Software.Sf_framecode NMRPipe _Software.Entry_ID 17095 _Software.ID 1 _Software.Name NMRPipe _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 17095 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 17095 1 stop_ save_ save_PIPP _Software.Sf_category software _Software.Sf_framecode PIPP _Software.Entry_ID 17095 _Software.ID 2 _Software.Name PIPP _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID Garrett . . 17095 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17095 2 stop_ save_ save_CAPP _Software.Sf_category software _Software.Sf_framecode CAPP _Software.Entry_ID 17095 _Software.ID 3 _Software.Name CAPP _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID Garrett . . 17095 3 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17095 3 stop_ save_ save_STAPP _Software.Sf_category software _Software.Sf_framecode STAPP _Software.Entry_ID 17095 _Software.ID 4 _Software.Name STAPP _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID '(Garrett et al., 1991)' . . 17095 4 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17095 4 stop_ save_ save_FRODO _Software.Sf_category software _Software.Sf_framecode FRODO _Software.Entry_ID 17095 _Software.ID 5 _Software.Name FRODO _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID '(Jones, 1978)' . . 17095 5 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'docking study' 17095 5 stop_ save_ save_GRASP _Software.Sf_category software _Software.Sf_framecode GRASP _Software.Entry_ID 17095 _Software.ID 6 _Software.Name GRASP _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID '(Nicholls et al., 1991)' . . 17095 6 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'docking study' 17095 6 stop_ save_ save_RIBBONS _Software.Sf_category software _Software.Sf_framecode RIBBONS _Software.Entry_ID 17095 _Software.ID 7 _Software.Name RIBBONS _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID '(Carson, 1987)' . . 17095 7 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID visualization 17095 7 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 17095 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 17095 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker DMX . 600 . . . 17095 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 17095 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 17095 1 2 '2D 1H-15N HSQC' no . . . . . . . . . . 2 $sample_2 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 17095 1 stop_ save_ save_Chemical_rate_list _Chemical_rate_list.Sf_category chemical_rates _Chemical_rate_list.Sf_framecode Chemical_rate_list _Chemical_rate_list.Entry_ID 17095 _Chemical_rate_list.ID 1 _Chemical_rate_list.Sample_condition_list_ID 1 _Chemical_rate_list.Sample_condition_list_label $sample_conditions_1 _Chemical_rate_list.Details . _Chemical_rate_list.Text_data_format . _Chemical_rate_list.Text_data . loop_ _Chemical_rate_experiment.Experiment_ID _Chemical_rate_experiment.Experiment_name _Chemical_rate_experiment.Sample_ID _Chemical_rate_experiment.Sample_label _Chemical_rate_experiment.Sample_state _Chemical_rate_experiment.Entry_ID _Chemical_rate_experiment.Chemical_rate_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17095 1 2 '2D 1H-15N HSQC' 2 $sample_2 isotropic 17095 1 stop_ loop_ _Chemical_rate_software.Software_ID _Chemical_rate_software.Software_label _Chemical_rate_software.Method_ID _Chemical_rate_software.Method_label _Chemical_rate_software.Entry_ID _Chemical_rate_software.Chemical_rate_list_ID 1 $NMRPipe . . 17095 1 2 $PIPP . . 17095 1 3 $CAPP . . 17095 1 4 $STAPP . . 17095 1 5 $FRODO . . 17095 1 6 $GRASP . . 17095 1 7 $RIBBONS . . 17095 1 stop_ loop_ _Chemical_rate.ID _Chemical_rate.Assembly_ID _Chemical_rate.Assembly_atom_ID _Chemical_rate.Entity_assembly_ID _Chemical_rate.Entity_ID _Chemical_rate.Comp_index_ID _Chemical_rate.Seq_ID _Chemical_rate.Comp_ID _Chemical_rate.Atom_ID _Chemical_rate.Atom_type _Chemical_rate.Atom_isotope_number _Chemical_rate.Val_type _Chemical_rate.Val _Chemical_rate.Val_min _Chemical_rate.Val_max _Chemical_rate.Val_err _Chemical_rate.Val_units _Chemical_rate.Resonance_ID _Chemical_rate.Auth_entity_assembly_ID _Chemical_rate.Auth_seq_ID _Chemical_rate.Auth_comp_ID _Chemical_rate.Auth_atom_ID _Chemical_rate.Entry_ID _Chemical_rate.Chemical_rate_list_ID 1 1 . 1 1 . . . . . . kass 15.000000 . . . MM-1 . . . . . 17095 1 2 1 . 1 1 . . . . . . Ka 0.150000 . . . MM-1 . . . . . 17095 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 17095 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17095 1 2 '2D 1H-15N HSQC' 2 $sample_2 isotropic 17095 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $NMRPipe . . 17095 1 2 $PIPP . . 17095 1 3 $CAPP . . 17095 1 4 $STAPP . . 17095 1 5 $FRODO . . 17095 1 6 $GRASP . . 17095 1 7 $RIBBONS . . 17095 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 . 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Ka 0.15 . MM-1 17095 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 EIN 1 $EIN 17095 1 1 1 2 HPr 2 $HPr 17095 1 stop_ save_