data_16962 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1J and 2J coupling constants in human oxidized ERp18 ; _BMRB_accession_number 16962 _BMRB_flat_file_name bmr16962.str _Entry_type original _Submission_date 2010-05-27 _Accession_date 2010-05-27 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Schmidt Jurgen M. . 2 Lohr Frank . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count coupling_constants 10 stop_ loop_ _Data_type _Data_type_count "coupling constants" 1268 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-01-19 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 15904 '1J coupling constants related to the Ca carbons in oxidized Flavodxin' 15905 '1J coupling constants related to the Ca carbons in Ribonuclease T1' 15906 '1J coupling constants related to the Ca carbons in Frataxin C-terminal domain' 15907 '1J coupling constants related to the Ca carbons in Ubiquitin' 15908 '1J coupling constants related to the Ca carbons in Xylanase' 15909 '1J coupling constants related to the Ca carbons in DFPase' 15964 'chemical shift assignments oxidized ERp18' 16579 '2J coupling constants in oxidized Flavodoxin' 16580 '2J coupling constants in Ribonuclease T1' 16581 '2J coupling constants in Frataxin C-terminal domain' 16582 '2J coupling constants in Ubiquitin' 16583 '2J coupling constants in Xylanase' 16584 '2J coupling constants in DFPase' stop_ _Original_release_date 2011-01-19 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'One-bond and two-bond j couplings help annotate protein secondary-structure motifs: J-coupling indexing applied to human endoplasmic reticulum protein ERp18.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 21117079 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Schmidt Jurgen M. . 2 Zhou Shen . . 3 Rowe Michelle L. . 4 Howard Mark J. . 5 Williamson Richard A. . 6 Lohr Frank . . stop_ _Journal_abbreviation Proteins _Journal_name_full 'Proteins: Structure, Function, and Bioinformatics' _Journal_volume 79 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 428 _Page_last 443 _Year 2011 _Details . loop_ _Keyword 'data mining' 'J-coupling index' 'one-bond coupling' 'secondary structure' statistics 'two-bond coupling' stop_ save_ ####################################### # Cited references within the entry # ####################################### save_Ottiger_et_al_1998 _Saveframe_category citation _Citation_full . _Citation_title 'Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 9571116 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ottiger M. . . 2 Delaglio F. . . 3 Bax A. . . stop_ _Journal_abbreviation 'J. Magn. Reson.' _Journal_name_full . _Journal_volume 131 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 373 _Page_last 378 _Year 1998 _Details . save_ save_Permi_et_al_1999 _Saveframe_category citation _Citation_full . _Citation_title 'Measurement of 1JNCO and 2JHNCO couplings from spin-state-selective two-dimensional correlation spectrum' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 10479547 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Permi P. . . 2 Heikkinen S. . . 3 Kilpelainen I. . . 4 Annila A. . . stop_ _Journal_abbreviation 'J. Magn. Reson.' _Journal_name_full . _Journal_volume 140 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 32 _Page_last 40 _Year 1999 _Details . save_ save_Yang_et_al_1999 _Saveframe_category citation _Citation_full . _Citation_title 'TROSY-based HNCO pulse sequence for the measurement of 1HN-15N, 15N-13CO, 1HN-13CO, 13CO-13Ca and 1HN-13Ca dipolar couplings in 15N, 13C, 2H-labeled proteins' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Yang D. . . 2 Venters R. A. . 3 Mueller G. A. . 4 Choi W. Y. . 5 Kay L. E. . stop_ _Journal_abbreviation 'J. Biomol. NMR' _Journal_name_full . _Journal_volume 14 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 333 _Page_last 343 _Year 1999 _Details . save_ save_Cornilescu_et_al_2000 _Saveframe_category citation _Citation_full . _Citation_title 'Large variations in one-bond 13Ca-13Cb J couplings in polypeptides correlate with backbone conformation' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Cornilescu G. . . 2 Bax A. M. . 3 Case D. A. . stop_ _Journal_abbreviation 'J. Am. Chem. Soc.' _Journal_name_full . _Journal_volume 122 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 2168 _Page_last 2171 _Year 2000 _Details . save_ save_Permi_&_Annila_2000 _Saveframe_category citation _Citation_full . _Citation_title 'Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 10805128 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Permi P. . . 2 Annila A. . . stop_ _Journal_abbreviation 'J. Biomol. NMR' _Journal_name_full . _Journal_volume 16 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 221 _Page_last 227 _Year 2000 _Details . save_ save_Hu_et_al_2003 _Saveframe_category citation _Citation_full . _Citation_title 'A high sensitivity 3D experiment for measuring Calpha-Halpha residual dipolar coupling constants' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hu W. . . 2 Zhang Z. . . 3 Chen Y. . . stop_ _Journal_abbreviation 'J. Magn. Reson.' _Journal_name_full . _Journal_volume 165 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 248 _Page_last 252 _Year 2003 _Details . save_ save_Ball_et_al_2006 _Saveframe_category citation _Citation_full . _Citation_title 'Measurement of one-bond 13Ca-1Ha residual dipolar coupling constants in proteins by selective manipulation of CaHa spins' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16495100 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ball G. . . 2 Meenan N. . . 3 Bromek K. . . 4 Smith B. O. . 5 Bella J. . . 6 Uhrin D. . . stop_ _Journal_abbreviation 'J. Magn. Reson.' _Journal_name_full . _Journal_volume 180 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 127 _Page_last 136 _Year 2006 _Details . save_ save_Nolis_et_al_2006 _Saveframe_category citation _Citation_full . _Citation_title 'Optimum spin-state selection for all multiplicities in the acquisition dimension of the HSQC experiment' _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16448830 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Nolis P. . . 2 Espinosa J. F. . 3 Parella T. . . stop_ _Journal_abbreviation 'J. Magn. Reson.' _Journal_name_full . _Journal_volume 180 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 39 _Page_last 50 _Year 2006 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name ERp18 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label ERp1 $ERp18 stop_ _System_molecular_weight 17774 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function oxidoreductase stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_ERp18 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common ERp18 _Molecular_mass 17774 _Mol_thiol_state 'all disulfide bound' loop_ _Biological_function oxidoreductase 'thioredoxin like' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 157 _Mol_residue_sequence ; MHHHHHHMSDGHNGLGKGFG DHIHWRTLEDGKKEAAASGL PLMVIIHKSWCGACKALKPK FAESTEISELSHNFVMVNLE DEEEPKDEDFSPDGGYIPRI LFLDPSGKVHPEIINENGNP SYKYFYVSAEQVVQGMKEAQ ERLTGDAFRKKHLEDEL ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 16 MET 2 17 HIS 3 18 HIS 4 19 HIS 5 20 HIS 6 21 HIS 7 22 HIS 8 23 MET 9 24 SER 10 25 ASP 11 26 GLY 12 27 HIS 13 28 ASN 14 29 GLY 15 30 LEU 16 31 GLY 17 32 LYS 18 33 GLY 19 34 PHE 20 35 GLY 21 36 ASP 22 37 HIS 23 38 ILE 24 39 HIS 25 40 TRP 26 41 ARG 27 42 THR 28 43 LEU 29 44 GLU 30 45 ASP 31 46 GLY 32 47 LYS 33 48 LYS 34 49 GLU 35 50 ALA 36 51 ALA 37 52 ALA 38 53 SER 39 54 GLY 40 55 LEU 41 56 PRO 42 57 LEU 43 58 MET 44 59 VAL 45 60 ILE 46 61 ILE 47 62 HIS 48 63 LYS 49 64 SER 50 65 TRP 51 66 CYS 52 67 GLY 53 68 ALA 54 69 CYS 55 70 LYS 56 71 ALA 57 72 LEU 58 73 LYS 59 74 PRO 60 75 LYS 61 76 PHE 62 77 ALA 63 78 GLU 64 79 SER 65 80 THR 66 81 GLU 67 82 ILE 68 83 SER 69 84 GLU 70 85 LEU 71 86 SER 72 87 HIS 73 88 ASN 74 89 PHE 75 90 VAL 76 91 MET 77 92 VAL 78 93 ASN 79 94 LEU 80 95 GLU 81 96 ASP 82 97 GLU 83 98 GLU 84 99 GLU 85 100 PRO 86 101 LYS 87 102 ASP 88 103 GLU 89 104 ASP 90 105 PHE 91 106 SER 92 107 PRO 93 108 ASP 94 109 GLY 95 110 GLY 96 111 TYR 97 112 ILE 98 113 PRO 99 114 ARG 100 115 ILE 101 116 LEU 102 117 PHE 103 118 LEU 104 119 ASP 105 120 PRO 106 121 SER 107 122 GLY 108 123 LYS 109 124 VAL 110 125 HIS 111 126 PRO 112 127 GLU 113 128 ILE 114 129 ILE 115 130 ASN 116 131 GLU 117 132 ASN 118 133 GLY 119 134 ASN 120 135 PRO 121 136 SER 122 137 TYR 123 138 LYS 124 139 TYR 125 140 PHE 126 141 TYR 127 142 VAL 128 143 SER 129 144 ALA 130 145 GLU 131 146 GLN 132 147 VAL 133 148 VAL 134 149 GLN 135 150 GLY 136 151 MET 137 152 LYS 138 153 GLU 139 154 ALA 140 155 GLN 141 156 GLU 142 157 ARG 143 158 LEU 144 159 THR 145 160 GLY 146 161 ASP 147 162 ALA 148 163 PHE 149 164 ARG 150 165 LYS 151 166 LYS 152 167 HIS 153 168 LEU 154 169 GLU 155 170 ASP 156 171 GLU 157 172 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-26 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15964 ERp18 100.00 157 100.00 100.00 1.83e-111 PDB 2K8V "Solution Structure Of Oxidised Erp18" 100.00 157 100.00 100.00 1.83e-111 DBJ BAG52132 "unnamed protein product [Homo sapiens]" 94.90 172 100.00 100.00 8.94e-105 GB AAD20035 "Unknown [Homo sapiens]" 94.90 172 100.00 100.00 8.94e-105 GB AAH01493 "Thioredoxin domain containing 12 (endoplasmic reticulum) [Homo sapiens]" 94.90 172 100.00 100.00 8.94e-105 GB AAH08913 "Thioredoxin domain containing 12 (endoplasmic reticulum) [Homo sapiens]" 94.90 172 99.33 99.33 2.12e-103 GB AAH08953 "Thioredoxin domain containing 12 (endoplasmic reticulum) [Homo sapiens]" 94.90 172 100.00 100.00 8.94e-105 GB AAN34781 "thioredoxin-like protein p19 [Homo sapiens]" 94.90 172 100.00 100.00 8.94e-105 REF NP_001253090 "thioredoxin domain containing 12 (endoplasmic reticulum) precursor [Macaca mulatta]" 94.90 172 99.33 100.00 1.45e-104 REF NP_056997 "thioredoxin domain-containing protein 12 precursor [Homo sapiens]" 94.90 172 100.00 100.00 8.94e-105 REF XP_001110583 "PREDICTED: thioredoxin domain-containing protein 12 [Macaca mulatta]" 94.90 208 98.66 100.00 1.30e-103 REF XP_002915769 "PREDICTED: thioredoxin domain-containing protein 12 isoform X1 [Ailuropoda melanoleuca]" 94.90 172 97.32 99.33 2.56e-102 REF XP_003364421 "PREDICTED: thioredoxin domain-containing protein 12-like isoform X1 [Equus caballus]" 94.90 172 97.99 99.33 8.78e-103 SP O95881 "RecName: Full=Thioredoxin domain-containing protein 12; AltName: Full=Endoplasmic reticulum resident protein 18; Short=ER prote" 94.90 172 100.00 100.00 8.94e-105 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $ERp18 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $ERp18 'recombinant technology' . Escherichia coli 'BL21(DE3) pLysS' pHIA128 'N-terminal His-tag' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_doubly_labeled _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $ERp18 0.7 mM '[U-95% 13C; U-95% 15N]' 'sodium phosphate' 20 mM 'natural abundance' 'sodium chloride' 100 mM 'natural abundance' D2O 10 % '[U-99% 2H]' H2O 90 % 'natural abundance' stop_ save_ save_singly_labeled _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $ERp18 1.0 mM '[U-95% 15N]' 'sodium phosphate' 20 mM 'natural abundance' 'sodium chloride' 100 mM 'natural abundance' D2O 10 % '[U-99% 2H]' H2O 90 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ save_jeval _Saveframe_category software _Name jeval _Version . loop_ _Vendor _Address _Electronic_address 'JM Schmidt' 'Dept. of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom' j.m.schmidt@kent.ac.uk stop_ loop_ _Task 'coupling constant extraction' 'data analysis' 'multiplet simulation' stop_ _Details 'Matlab-based suite of utilities for the display, selection, and simulation of 2D multiplet projections from multi-dimensional NMR spectra' save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details 'room-temperature 1H{13C,15N}-triple-resonance three-axis PFG probe' save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 500 _Details 'room-temperature 1H{13C,15N}-triple-resonance three-axis PFG probe' save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details 'cryogenically cooled 1H{13C,15N}-triple-resonance z-gradient probe' save_ save_spectrometer_4 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details 'cryogenically cooled 1H{13C,15N}-triple-resonance z-gradient probe' save_ save_spectrometer_5 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details 'cryogenically cooled 1H{13C,15N}-triple-resonance z-gradient probe' save_ save_spectrometer_6 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 900 _Details 'cryogenically cooled 1H{13C,15N}-triple-resonance z-gradient probe' save_ ############################# # NMR applied experiments # ############################# save_3D_Ca-coupled_[15N,1H]-TROSY-HNCO_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Ca-coupled [15N,1H]-TROSY-HNCO' _Sample_label $doubly_labeled save_ save_3D_[1Ha,13Ca]-multiple-quantum_HCAN_quantitative_J-correlation_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D [1Ha,13Ca]-multiple-quantum HCAN quantitative J-correlation' _Sample_label $doubly_labeled save_ save_3D_Cb-coupled_[15N,1H]-TROSY-HN(CO)CA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA' _Sample_label $doubly_labeled save_ save_3D_Ca-coupled_H(CACO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Ca-coupled H(CACO)NH' _Sample_label $doubly_labeled save_ save_3D_Ha-coupled_[15N,1H]-TROSY-H(N)COCA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Ha-coupled [15N,1H]-TROSY-H(N)COCA' _Sample_label $doubly_labeled save_ save_3D_Ha-coupled_[15N,1H]-TROSY-HN(CO)CA_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Ha-coupled [15N,1H]-TROSY-HN(CO)CA' _Sample_label $doubly_labeled save_ save_2D_ct-[13C,1H]-IPAP-HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D ct-[13C,1H]-IPAP-HSQC' _Sample_label $doubly_labeled save_ save_2D_IPAP-type_HN(CO-a/b-NCa-J)-TROSY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D IPAP-type HN(CO-a/b-NCa-J)-TROSY' _Sample_label $doubly_labeled save_ save_2D_IPAP-type_HN(a/b-NCO-J)-TROSY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D IPAP-type HN(a/b-NCO-J)-TROSY' _Sample_label $doubly_labeled save_ save_2D_[15N,1H]-IPAP-HSQC_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D [15N,1H]-IPAP-HSQC' _Sample_label $singly_labeled save_ save_3D_Ca-coupled_15N_1H-TROSY-HNCO _Saveframe_category NMR_applied_experiment _Experiment_name '3D Ca-coupled [15N,1H]-TROSY-HNCO' _BMRB_pulse_sequence_accession_number . _Details ; 1JCACO measurement; Yang et al., 1999 ; save_ save_3D_Cb-coupled_15N_1H-TROSY-HN_CO_CA _Saveframe_category NMR_applied_experiment _Experiment_name '3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA' _BMRB_pulse_sequence_accession_number . _Details ; 1JCACB measurement; Cornilescu et al., 2000 ; save_ save_3D_1Ha_13Ca-multiple-quantum_HCAN_quantitative_J-correlation _Saveframe_category NMR_applied_experiment _Experiment_name '3D [1Ha,13Ca]-multiple-quantum HCAN quantitative J-correlation' _BMRB_pulse_sequence_accession_number . _Details ; 1JCAN measurement in proline; F. Lohr, unpublished ; save_ save_3D_Ca-coupled_H_CACO_NH _Saveframe_category NMR_applied_experiment _Experiment_name 3D_Ca-coupled_H(CACO)NH _BMRB_pulse_sequence_accession_number . _Details ; 1JCAHA measurement; Hu et al., 2003; Ball et al., 2006 ; save_ save_3D_Ha-coupled_15N_1H-TROSY-H_N_COCA _Saveframe_category NMR_applied_experiment _Experiment_name 3D_Ha-coupled_[15N,1H]-TROSY-H(N)COCA _BMRB_pulse_sequence_accession_number . _Details ; 2JHACO measurement; F. Lohr, unpublished ; save_ save_3D_Ha-coupled_ct-15N_1H-TROSY-HN_CO_CA _Saveframe_category NMR_applied_experiment _Experiment_name 3D_Ha-coupled_ct-[15N,1H]-TROSY-HN(CO)CA _BMRB_pulse_sequence_accession_number . _Details ; 1JCAHA measurement; 14-ms 13Ca evolution ; save_ save_2D_ct-13C_1H-IPAP-HSQC _Saveframe_category NMR_applied_experiment _Experiment_name 2D_ct-[13C,1H]-IPAP-HSQC _BMRB_pulse_sequence_accession_number . _Details ; 1JCaHa measurement; Nolis et al., 2006 ; save_ save_2D_IPAP-type_HN_CO-a_b-NCa-J-TROSY _Saveframe_category NMR_applied_experiment _Experiment_name 2D_IPAP-type_HN(CO-a/b-NCa-J)-TROSY _BMRB_pulse_sequence_accession_number . _Details ; 1JNCa and 2JCaN and 2JHNCa measurement; 128-ms 15N acquisition time; Permi & Annila, 2000 ; save_ save_2D_IPAP-type_HN_a_b-NCO-J-TROSY _Saveframe_category NMR_applied_experiment _Experiment_name 2D_IPAP-type_HN(a/b-NCO-J)-TROSY _BMRB_pulse_sequence_accession_number . _Details ; 1JCON and 2JCOHN measurement; 120-ms 15N acquisition time; Permi et al., 1999 ; save_ save_2D_15N_1H-IPAP-HSQC _Saveframe_category NMR_applied_experiment _Experiment_name 2D_[15N,1H]-IPAP-HSQC _BMRB_pulse_sequence_accession_number . _Details ; 1JNHN measurement; Ottiger et al., 1998 ; save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 303 . K stop_ save_ ######################## # Coupling constants # ######################## save_1JCaCO _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '3D Ca-coupled [15N,1H]-TROSY-HNCO' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 500 _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JCACO 7 HIS CA 7 HIS C 53.19 . . 0.50 2 1JCACO 8 MET CA 8 MET C 51.08 . . 0.50 3 1JCACO 9 SER CA 9 SER C 52.71 . . 0.50 4 1JCACO 10 ASP CA 10 ASP C 52.10 . . 0.50 5 1JCACO 12 HIS CA 12 HIS C 50.85 . . 0.50 6 1JCACO 13 ASN CA 13 ASN C 52.68 . . 0.50 7 1JCACO 14 GLY CA 14 GLY C 52.44 . . 0.50 8 1JCACO 15 LEU CA 15 LEU C 53.26 . . 0.50 9 1JCACO 16 GLY CA 16 GLY C 51.26 . . 0.50 10 1JCACO 17 LYS CA 17 LYS C 53.84 . . 0.50 11 1JCACO 18 GLY CA 18 GLY C 51.30 . . 0.50 12 1JCACO 19 PHE CA 19 PHE C 52.33 . . 0.50 13 1JCACO 20 GLY CA 20 GLY C 54.74 . . 0.50 14 1JCACO 21 ASP CA 21 ASP C 52.72 . . 0.50 15 1JCACO 22 HIS CA 22 HIS C 52.20 . . 0.50 16 1JCACO 23 ILE CA 23 ILE C 52.37 . . 0.50 17 1JCACO 24 HIS CA 24 HIS C 52.15 . . 0.50 18 1JCACO 25 TRP CA 25 TRP C 51.82 . . 0.50 19 1JCACO 26 ARG CA 26 ARG C 51.81 . . 0.50 20 1JCACO 27 THR CA 27 THR C 49.74 . . 0.50 21 1JCACO 28 LEU CA 28 LEU C 52.52 . . 0.50 22 1JCACO 29 GLU CA 29 GLU C 52.70 . . 0.50 23 1JCACO 30 ASP CA 30 ASP C 52.89 . . 0.50 24 1JCACO 31 GLY CA 31 GLY C 51.61 . . 0.50 25 1JCACO 32 LYS CA 32 LYS C 52.61 . . 0.50 26 1JCACO 33 LYS CA 33 LYS C 52.29 . . 0.50 27 1JCACO 34 GLU CA 34 GLU C 51.52 . . 0.50 28 1JCACO 35 ALA CA 35 ALA C 53.88 . . 0.50 29 1JCACO 36 ALA CA 36 ALA C 52.65 . . 0.50 30 1JCACO 37 ALA CA 37 ALA C 52.42 . . 0.50 31 1JCACO 38 SER CA 38 SER C 52.86 . . 0.50 32 1JCACO 39 GLY CA 39 GLY C 51.57 . . 0.50 33 1JCACO 41 PRO CA 41 PRO C 52.19 . . 0.50 34 1JCACO 42 LEU CA 42 LEU C 50.77 . . 0.50 35 1JCACO 43 MET CA 43 MET C 52.52 . . 0.50 36 1JCACO 44 VAL CA 44 VAL C 52.51 . . 0.50 37 1JCACO 45 ILE CA 45 ILE C 51.54 . . 0.50 38 1JCACO 46 ILE CA 46 ILE C 52.10 . . 0.50 39 1JCACO 47 HIS CA 47 HIS C 53.15 . . 0.50 40 1JCACO 48 LYS CA 48 LYS C 49.15 . . 0.50 41 1JCACO 49 SER CA 49 SER C 51.02 . . 0.50 42 1JCACO 53 ALA CA 53 ALA C 51.86 . . 0.50 43 1JCACO 54 CYS CA 54 CYS C 51.32 . . 0.50 44 1JCACO 55 LYS CA 55 LYS C 51.96 . . 0.50 45 1JCACO 56 ALA CA 56 ALA C 52.04 . . 0.50 46 1JCACO 57 LEU CA 57 LEU C 52.29 . . 0.50 47 1JCACO 59 PRO CA 59 PRO C 53.50 . . 0.50 48 1JCACO 60 LYS CA 60 LYS C 53.24 . . 0.50 49 1JCACO 61 PHE CA 61 PHE C 52.89 . . 0.50 50 1JCACO 62 ALA CA 62 ALA C 52.62 . . 0.50 51 1JCACO 63 GLU CA 63 GLU C 52.31 . . 0.50 52 1JCACO 64 SER CA 64 SER C 51.62 . . 0.50 53 1JCACO 65 THR CA 65 THR C 52.55 . . 0.50 54 1JCACO 66 GLU CA 66 GLU C 52.98 . . 0.50 55 1JCACO 67 ILE CA 67 ILE C 52.50 . . 0.50 56 1JCACO 68 SER CA 68 SER C 50.91 . . 0.50 57 1JCACO 69 GLU CA 69 GLU C 52.05 . . 0.50 58 1JCACO 70 LEU CA 70 LEU C 53.22 . . 0.50 59 1JCACO 71 SER CA 71 SER C 51.02 . . 0.50 60 1JCACO 72 HIS CA 72 HIS C 53.57 . . 0.50 61 1JCACO 73 ASN CA 73 ASN C 52.85 . . 0.50 62 1JCACO 74 PHE CA 74 PHE C 51.55 . . 0.50 63 1JCACO 75 VAL CA 75 VAL C 52.40 . . 0.50 64 1JCACO 76 MET CA 76 MET C 54.35 . . 0.50 65 1JCACO 77 VAL CA 77 VAL C 53.38 . . 0.50 66 1JCACO 78 ASN CA 78 ASN C 51.93 . . 0.50 67 1JCACO 79 LEU CA 79 LEU C 53.44 . . 0.50 68 1JCACO 80 GLU CA 80 GLU C 50.33 . . 0.50 69 1JCACO 81 ASP CA 81 ASP C 51.17 . . 0.50 70 1JCACO 82 GLU CA 82 GLU C 51.20 . . 0.50 71 1JCACO 83 GLU CA 83 GLU C 52.22 . . 0.50 72 1JCACO 85 PRO CA 85 PRO C 54.33 . . 0.50 73 1JCACO 86 LYS CA 86 LYS C 51.88 . . 0.50 74 1JCACO 87 ASP CA 87 ASP C 54.52 . . 0.50 75 1JCACO 88 GLU CA 88 GLU C 52.22 . . 0.50 76 1JCACO 89 ASP CA 89 ASP C 50.26 . . 0.50 77 1JCACO 90 PHE CA 90 PHE C 51.81 . . 0.50 78 1JCACO 92 PRO CA 92 PRO C 52.28 . . 0.50 79 1JCACO 93 ASP CA 93 ASP C 53.27 . . 0.50 80 1JCACO 94 GLY CA 94 GLY C 50.27 . . 0.50 81 1JCACO 95 GLY CA 95 GLY C 52.88 . . 0.50 82 1JCACO 96 TYR CA 96 TYR C 52.47 . . 0.50 83 1JCACO 98 PRO CA 98 PRO C 52.60 . . 0.50 84 1JCACO 99 ARG CA 99 ARG C 52.45 . . 0.50 85 1JCACO 100 ILE CA 100 ILE C 54.99 . . 0.50 86 1JCACO 101 LEU CA 101 LEU C 54.47 . . 0.50 87 1JCACO 102 PHE CA 102 PHE C 54.95 . . 0.50 88 1JCACO 103 LEU CA 103 LEU C 51.36 . . 0.50 89 1JCACO 105 PRO CA 105 PRO C 55.07 . . 0.50 90 1JCACO 106 SER CA 106 SER C 51.59 . . 0.50 91 1JCACO 107 GLY CA 107 GLY C 52.60 . . 0.50 92 1JCACO 108 LYS CA 108 LYS C 52.31 . . 0.50 93 1JCACO 109 VAL CA 109 VAL C 53.63 . . 0.50 94 1JCACO 111 PRO CA 111 PRO C 50.55 . . 0.50 95 1JCACO 112 GLU CA 112 GLU C 50.92 . . 0.50 96 1JCACO 113 ILE CA 113 ILE C 51.89 . . 0.50 97 1JCACO 114 ILE CA 114 ILE C 51.73 . . 0.50 98 1JCACO 115 ASN CA 115 ASN C 52.04 . . 0.50 99 1JCACO 116 GLU CA 116 GLU C 52.81 . . 0.50 100 1JCACO 117 ASN CA 117 ASN C 53.09 . . 0.50 101 1JCACO 118 GLY CA 118 GLY C 51.87 . . 0.50 102 1JCACO 120 PRO CA 120 PRO C 53.85 . . 0.50 103 1JCACO 121 SER CA 121 SER C 50.98 . . 0.50 104 1JCACO 122 TYR CA 122 TYR C 51.01 . . 0.50 105 1JCACO 123 LYS CA 123 LYS C 52.37 . . 0.50 106 1JCACO 124 TYR CA 124 TYR C 52.55 . . 0.50 107 1JCACO 125 PHE CA 125 PHE C 54.19 . . 0.50 108 1JCACO 126 TYR CA 126 TYR C 50.70 . . 0.50 109 1JCACO 127 VAL CA 127 VAL C 53.23 . . 0.50 110 1JCACO 128 SER CA 128 SER C 53.67 . . 0.50 111 1JCACO 129 ALA CA 129 ALA C 52.56 . . 0.50 112 1JCACO 130 GLU CA 130 GLU C 53.01 . . 0.50 113 1JCACO 131 GLN CA 131 GLN C 51.73 . . 0.50 114 1JCACO 132 VAL CA 132 VAL C 52.14 . . 0.50 115 1JCACO 133 VAL CA 133 VAL C 53.13 . . 0.50 116 1JCACO 134 GLN CA 134 GLN C 51.71 . . 0.50 117 1JCACO 135 GLY CA 135 GLY C 52.10 . . 0.50 118 1JCACO 136 MET CA 136 MET C 52.17 . . 0.50 119 1JCACO 137 LYS CA 137 LYS C 52.35 . . 0.50 120 1JCACO 138 GLU CA 138 GLU C 51.86 . . 0.50 121 1JCACO 139 ALA CA 139 ALA C 52.97 . . 0.50 122 1JCACO 140 GLN CA 140 GLN C 51.93 . . 0.50 123 1JCACO 141 GLU CA 141 GLU C 52.32 . . 0.50 124 1JCACO 142 ARG CA 142 ARG C 53.37 . . 0.50 125 1JCACO 143 LEU CA 143 LEU C 53.49 . . 0.50 126 1JCACO 144 THR CA 144 THR C 52.01 . . 0.50 127 1JCACO 145 GLY CA 145 GLY C 52.16 . . 0.50 128 1JCACO 146 ASP CA 146 ASP C 53.12 . . 0.50 129 1JCACO 147 ALA CA 147 ALA C 52.64 . . 0.50 130 1JCACO 148 PHE CA 148 PHE C 53.00 . . 0.50 131 1JCACO 149 ARG CA 149 ARG C 51.02 . . 0.50 132 1JCACO 150 LYS CA 150 LYS C 52.34 . . 0.50 133 1JCACO 151 LYS CA 151 LYS C 51.67 . . 0.50 134 1JCACO 152 HIS CA 152 HIS C 52.33 . . 0.50 135 1JCACO 153 LEU CA 153 LEU C 52.37 . . 0.50 136 1JCACO 154 GLU CA 154 GLU C 52.30 . . 0.50 137 1JCACO 155 ASP CA 155 ASP C 52.21 . . 0.50 138 1JCACO 156 GLU CA 156 GLU C 52.32 . . 0.50 stop_ save_ save_1JCaN_ _Saveframe_category coupling_constants _Details 'MQ-HCAN experiment for proline residues, IPAP-TROSY experiment for all other' loop_ _Experiment_label '3D [1Ha,13Ca]-multiple-quantum HCAN quantitative J-correlation' '2D IPAP-type HN(CO-a/b-NCa-J)-TROSY' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JCAN_ 8 MET CA 8 MET N -10.37 . . 0.50 2 1JCAN_ 9 SER CA 9 SER N -10.87 . . 0.50 3 1JCAN_ 10 ASP CA 10 ASP N -10.22 . . 0.50 4 1JCAN_ 11 GLY CA 11 GLY N -11.19 . . 0.50 5 1JCAN_ 13 ASN CA 13 ASN N -8.99 . . 0.50 6 1JCAN_ 14 GLY CA 14 GLY N -11.53 . . 0.50 7 1JCAN_ 15 LEU CA 15 LEU N -9.46 . . 0.50 8 1JCAN_ 16 GLY CA 16 GLY N -10.04 . . 0.50 9 1JCAN_ 17 LYS CA 17 LYS N -9.53 . . 0.50 10 1JCAN_ 18 GLY CA 18 GLY N -10.69 . . 0.50 11 1JCAN_ 19 PHE CA 19 PHE N -9.47 . . 0.50 12 1JCAN_ 20 GLY CA 20 GLY N -11.71 . . 0.50 13 1JCAN_ 21 ASP CA 21 ASP N -12.24 . . 0.50 14 1JCAN_ 22 HIS CA 22 HIS N -8.44 . . 0.50 15 1JCAN_ 23 ILE CA 23 ILE N -10.53 . . 0.50 16 1JCAN_ 24 HIS CA 24 HIS N -10.15 . . 0.50 17 1JCAN_ 25 TRP CA 25 TRP N -11.56 . . 0.50 18 1JCAN_ 26 ARG CA 26 ARG N -10.94 . . 0.50 19 1JCAN_ 27 THR CA 27 THR N -11.29 . . 0.50 20 1JCAN_ 28 LEU CA 28 LEU N -8.96 . . 0.50 21 1JCAN_ 29 GLU CA 29 GLU N -8.84 . . 0.50 22 1JCAN_ 30 ASP CA 30 ASP N -9.13 . . 0.50 23 1JCAN_ 31 GLY CA 31 GLY N -9.82 . . 0.50 24 1JCAN_ 32 LYS CA 32 LYS N -9.92 . . 0.50 25 1JCAN_ 33 LYS CA 33 LYS N -8.94 . . 0.50 26 1JCAN_ 34 GLU CA 34 GLU N -9.27 . . 0.50 27 1JCAN_ 35 ALA CA 35 ALA N -9.14 . . 0.50 28 1JCAN_ 36 ALA CA 36 ALA N -9.75 . . 0.50 29 1JCAN_ 37 ALA CA 37 ALA N -9.86 . . 0.50 30 1JCAN_ 38 SER CA 38 SER N -10.17 . . 0.50 31 1JCAN_ 39 GLY CA 39 GLY N -9.97 . . 0.50 32 1JCAN_ 40 LEU CA 40 LEU N -10.48 . . 0.50 33 1JCAN_ 42 LEU CA 42 LEU N -9.94 . . 0.50 34 1JCAN_ 43 MET CA 43 MET N -9.69 . . 0.50 35 1JCAN_ 44 VAL CA 44 VAL N -10.01 . . 0.50 36 1JCAN_ 45 ILE CA 45 ILE N -9.41 . . 0.50 37 1JCAN_ 46 ILE CA 46 ILE N -9.67 . . 0.50 38 1JCAN_ 47 HIS CA 47 HIS N -13.10 . . 0.50 39 1JCAN_ 48 LYS CA 48 LYS N -11.30 . . 0.50 40 1JCAN_ 49 SER CA 49 SER N -8.74 . . 0.50 41 1JCAN_ 50 TRP CA 50 TRP N -11.02 . . 0.50 42 1JCAN_ 54 CYS CA 54 CYS N -9.75 . . 0.50 43 1JCAN_ 55 LYS CA 55 LYS N -9.53 . . 0.50 44 1JCAN_ 56 ALA CA 56 ALA N -9.57 . . 0.50 45 1JCAN_ 57 LEU CA 57 LEU N -9.11 . . 0.50 46 1JCAN_ 58 LYS CA 58 LYS N -9.09 . . 0.50 47 1JCAN_ 59 PRO CA 59 PRO N -9.05 . . 0.50 48 1JCAN_ 60 LYS CA 60 LYS N -9.28 . . 0.50 49 1JCAN_ 61 PHE CA 61 PHE N -9.35 . . 0.50 50 1JCAN_ 62 ALA CA 62 ALA N -9.48 . . 0.50 51 1JCAN_ 63 GLU CA 63 GLU N -9.04 . . 0.50 52 1JCAN_ 64 SER CA 64 SER N -10.74 . . 0.50 53 1JCAN_ 66 GLU CA 66 GLU N -8.57 . . 0.50 54 1JCAN_ 67 ILE CA 67 ILE N -8.54 . . 0.50 55 1JCAN_ 68 SER CA 68 SER N -9.58 . . 0.50 56 1JCAN_ 70 LEU CA 70 LEU N -10.51 . . 0.50 57 1JCAN_ 71 SER CA 71 SER N -8.70 . . 0.50 58 1JCAN_ 72 HIS CA 72 HIS N -10.19 . . 0.50 59 1JCAN_ 73 ASN CA 73 ASN N -9.31 . . 0.50 60 1JCAN_ 74 PHE CA 74 PHE N -12.17 . . 0.50 61 1JCAN_ 75 VAL CA 75 VAL N -9.89 . . 0.50 62 1JCAN_ 76 MET CA 76 MET N -9.74 . . 0.50 63 1JCAN_ 77 VAL CA 77 VAL N -10.73 . . 0.50 64 1JCAN_ 78 ASN CA 78 ASN N -10.43 . . 0.50 65 1JCAN_ 79 LEU CA 79 LEU N -8.70 . . 0.50 66 1JCAN_ 80 GLU CA 80 GLU N -10.24 . . 0.50 67 1JCAN_ 81 ASP CA 81 ASP N -10.39 . . 0.50 68 1JCAN_ 82 GLU CA 82 GLU N -9.38 . . 0.50 69 1JCAN_ 83 GLU CA 83 GLU N -8.87 . . 0.50 70 1JCAN_ 84 GLU CA 84 GLU N -10.40 . . 0.50 71 1JCAN_ 85 PRO CA 85 PRO N -11.27 . . 0.50 72 1JCAN_ 86 LYS CA 86 LYS N -9.69 . . 0.50 73 1JCAN_ 87 ASP CA 87 ASP N -10.04 . . 0.50 74 1JCAN_ 89 ASP CA 89 ASP N -8.86 . . 0.50 75 1JCAN_ 90 PHE CA 90 PHE N -9.44 . . 0.50 76 1JCAN_ 91 SER CA 91 SER N -9.85 . . 0.50 77 1JCAN_ 92 PRO CA 92 PRO N -9.04 . . 0.50 78 1JCAN_ 93 ASP CA 93 ASP N -9.65 . . 0.50 79 1JCAN_ 94 GLY CA 94 GLY N -13.14 . . 0.50 80 1JCAN_ 95 GLY CA 95 GLY N -10.34 . . 0.50 81 1JCAN_ 96 TYR CA 96 TYR N -12.49 . . 0.50 82 1JCAN_ 100 ILE CA 100 ILE N -9.44 . . 0.50 83 1JCAN_ 101 LEU CA 101 LEU N -10.29 . . 0.50 84 1JCAN_ 102 PHE CA 102 PHE N -11.23 . . 0.50 85 1JCAN_ 103 LEU CA 103 LEU N -9.90 . . 0.50 86 1JCAN_ 104 ASP CA 104 ASP N -10.99 . . 0.50 87 1JCAN_ 105 PRO CA 105 PRO N -9.04 . . 0.50 88 1JCAN_ 106 SER CA 106 SER N -9.70 . . 0.50 89 1JCAN_ 107 GLY CA 107 GLY N -10.20 . . 0.50 90 1JCAN_ 108 LYS CA 108 LYS N -10.89 . . 0.50 91 1JCAN_ 109 VAL CA 109 VAL N -11.77 . . 0.50 92 1JCAN_ 110 HIS CA 110 HIS N -10.30 . . 0.50 93 1JCAN_ 111 PRO CA 111 PRO N -9.73 . . 0.50 94 1JCAN_ 112 GLU CA 112 GLU N -8.58 . . 0.50 95 1JCAN_ 113 ILE CA 113 ILE N -8.63 . . 0.50 96 1JCAN_ 114 ILE CA 114 ILE N -10.16 . . 0.50 97 1JCAN_ 115 ASN CA 115 ASN N -9.51 . . 0.50 98 1JCAN_ 116 GLU CA 116 GLU N -8.76 . . 0.50 99 1JCAN_ 117 ASN CA 117 ASN N -9.28 . . 0.50 100 1JCAN_ 118 GLY CA 118 GLY N -12.28 . . 0.50 101 1JCAN_ 119 ASN CA 119 ASN N -10.29 . . 0.50 102 1JCAN_ 120 PRO CA 120 PRO N -9.00 . . 0.50 103 1JCAN_ 122 TYR CA 122 TYR N -9.47 . . 0.50 104 1JCAN_ 123 LYS CA 123 LYS N -8.80 . . 0.50 105 1JCAN_ 124 TYR CA 124 TYR N -10.35 . . 0.50 106 1JCAN_ 125 PHE CA 125 PHE N -10.40 . . 0.50 107 1JCAN_ 126 TYR CA 126 TYR N -10.72 . . 0.50 108 1JCAN_ 127 VAL CA 127 VAL N -8.24 . . 0.50 109 1JCAN_ 128 SER CA 128 SER N -12.51 . . 0.50 110 1JCAN_ 129 ALA CA 129 ALA N -9.15 . . 0.50 111 1JCAN_ 130 GLU CA 130 GLU N -9.38 . . 0.50 112 1JCAN_ 131 GLN CA 131 GLN N -9.12 . . 0.50 113 1JCAN_ 132 VAL CA 132 VAL N -9.13 . . 0.50 114 1JCAN_ 133 VAL CA 133 VAL N -9.40 . . 0.50 115 1JCAN_ 134 GLN CA 134 GLN N -9.14 . . 0.50 116 1JCAN_ 135 GLY CA 135 GLY N -9.99 . . 0.50 117 1JCAN_ 136 MET CA 136 MET N -9.35 . . 0.50 118 1JCAN_ 137 LYS CA 137 LYS N -9.73 . . 0.50 119 1JCAN_ 138 GLU CA 138 GLU N -8.62 . . 0.50 120 1JCAN_ 139 ALA CA 139 ALA N -9.40 . . 0.50 121 1JCAN_ 140 GLN CA 140 GLN N -9.11 . . 0.50 122 1JCAN_ 141 GLU CA 141 GLU N -8.83 . . 0.50 123 1JCAN_ 142 ARG CA 142 ARG N -9.18 . . 0.50 124 1JCAN_ 144 THR CA 144 THR N -11.04 . . 0.50 125 1JCAN_ 145 GLY CA 145 GLY N -11.57 . . 0.50 126 1JCAN_ 146 ASP CA 146 ASP N -9.80 . . 0.50 127 1JCAN_ 147 ALA CA 147 ALA N -9.80 . . 0.50 128 1JCAN_ 148 PHE CA 148 PHE N -10.03 . . 0.50 129 1JCAN_ 149 ARG CA 149 ARG N -9.71 . . 0.50 130 1JCAN_ 150 LYS CA 150 LYS N -10.12 . . 0.50 131 1JCAN_ 151 LYS CA 151 LYS N -10.31 . . 0.50 132 1JCAN_ 152 HIS CA 152 HIS N -10.39 . . 0.50 133 1JCAN_ 153 LEU CA 153 LEU N -9.30 . . 0.50 134 1JCAN_ 154 GLU CA 154 GLU N -9.53 . . 0.50 135 1JCAN_ 155 ASP CA 155 ASP N -9.94 . . 0.50 136 1JCAN_ 156 GLU CA 156 GLU N -9.64 . . 0.50 137 1JCAN_ 157 LEU CA 157 LEU N -9.19 . . 0.50 stop_ save_ save_1JCaHa _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '3D Ca-coupled H(CACO)NH' '3D Ha-coupled [15N,1H]-TROSY-HN(CO)CA' '2D ct-[13C,1H]-IPAP-HSQC' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JCAHA 9 SER CA 9 SER HA 142.13 . . 0.50 2 1JCAHA 10 ASP CA 10 ASP HA 142.40 . . 0.50 3 1JCAHA 13 ASN CA 13 ASN HA 143.12 . . 0.50 4 1JCAHA 15 LEU CA 15 LEU HA 141.47 . . 0.50 5 1JCAHA 17 LYS CA 17 LYS HA 137.43 . . 0.50 6 1JCAHA 19 PHE CA 19 PHE HA 140.73 . . 0.50 7 1JCAHA 21 ASP CA 21 ASP HA 138.13 . . 0.50 8 1JCAHA 22 HIS CA 22 HIS HA 145.38 . . 0.50 9 1JCAHA 23 ILE CA 23 ILE HA 140.04 . . 0.50 10 1JCAHA 24 HIS CA 24 HIS HA 140.14 . . 0.50 11 1JCAHA 25 TRP CA 25 TRP HA 145.58 . . 0.50 12 1JCAHA 26 ARG CA 26 ARG HA 139.00 . . 0.50 13 1JCAHA 27 THR CA 27 THR HA 141.54 . . 0.50 14 1JCAHA 28 LEU CA 28 LEU HA 145.07 . . 0.50 15 1JCAHA 29 GLU CA 29 GLU HA 143.23 . . 0.50 16 1JCAHA 30 ASP CA 30 ASP HA 149.45 . . 0.50 17 1JCAHA 32 LYS CA 32 LYS HA 147.86 . . 0.50 18 1JCAHA 33 LYS CA 33 LYS HA 149.34 . . 0.50 19 1JCAHA 34 GLU CA 34 GLU HA 147.48 . . 0.50 20 1JCAHA 35 ALA CA 35 ALA HA 149.07 . . 0.50 21 1JCAHA 36 ALA CA 36 ALA HA 148.92 . . 0.50 22 1JCAHA 37 ALA CA 37 ALA HA 147.32 . . 0.50 23 1JCAHA 38 SER CA 38 SER HA 144.92 . . 0.50 24 1JCAHA 40 LEU CA 40 LEU HA 140.78 . . 0.50 25 1JCAHA 41 PRO CA 41 PRO HA 144.19 . . 0.50 26 1JCAHA 42 LEU CA 42 LEU HA 138.47 . . 0.50 27 1JCAHA 43 MET CA 43 MET HA 143.43 . . 0.50 28 1JCAHA 44 VAL CA 44 VAL HA 138.39 . . 0.50 29 1JCAHA 45 ILE CA 45 ILE HA 138.40 . . 0.50 30 1JCAHA 46 ILE CA 46 ILE HA 142.79 . . 0.50 31 1JCAHA 47 HIS CA 47 HIS HA 142.32 . . 0.50 32 1JCAHA 48 LYS CA 48 LYS HA 141.73 . . 0.50 33 1JCAHA 49 SER CA 49 SER HA 141.99 . . 0.50 34 1JCAHA 50 TRP CA 50 TRP HA 146.40 . . 0.50 35 1JCAHA 53 ALA CA 53 ALA HA 150.40 . . 0.50 36 1JCAHA 54 CYS CA 54 CYS HA 151.54 . . 0.50 37 1JCAHA 55 LYS CA 55 LYS HA 145.86 . . 0.50 38 1JCAHA 56 ALA CA 56 ALA HA 148.67 . . 0.50 39 1JCAHA 57 LEU CA 57 LEU HA 148.32 . . 0.50 40 1JCAHA 58 LYS CA 58 LYS HA 142.52 . . 0.50 41 1JCAHA 59 PRO CA 59 PRO HA 150.10 . . 0.50 42 1JCAHA 60 LYS CA 60 LYS HA 147.67 . . 0.50 43 1JCAHA 61 PHE CA 61 PHE HA 151.31 . . 0.50 44 1JCAHA 62 ALA CA 62 ALA HA 147.74 . . 0.50 45 1JCAHA 63 GLU CA 63 GLU HA 141.72 . . 0.50 46 1JCAHA 64 SER CA 64 SER HA 137.13 . . 0.50 47 1JCAHA 65 THR CA 65 THR HA 144.61 . . 0.50 48 1JCAHA 66 GLU CA 66 GLU HA 150.94 . . 0.50 49 1JCAHA 67 ILE CA 67 ILE HA 146.41 . . 0.50 50 1JCAHA 68 SER CA 68 SER HA 145.26 . . 0.50 51 1JCAHA 69 GLU CA 69 GLU HA 147.25 . . 0.50 52 1JCAHA 70 LEU CA 70 LEU HA 145.03 . . 0.50 53 1JCAHA 71 SER CA 71 SER HA 147.83 . . 0.50 54 1JCAHA 72 HIS CA 72 HIS HA 145.01 . . 0.50 55 1JCAHA 73 ASN CA 73 ASN HA 144.86 . . 0.50 56 1JCAHA 74 PHE CA 74 PHE HA 144.87 . . 0.50 57 1JCAHA 75 VAL CA 75 VAL HA 135.01 . . 0.50 58 1JCAHA 76 MET CA 76 MET HA 134.80 . . 0.50 59 1JCAHA 77 VAL CA 77 VAL HA 143.42 . . 0.50 60 1JCAHA 78 ASN CA 78 ASN HA 138.76 . . 0.50 61 1JCAHA 79 LEU CA 79 LEU HA 142.61 . . 0.50 62 1JCAHA 80 GLU CA 80 GLU HA 136.84 . . 0.50 63 1JCAHA 81 ASP CA 81 ASP HA 142.68 . . 0.50 64 1JCAHA 82 GLU CA 82 GLU HA 140.90 . . 0.50 65 1JCAHA 83 GLU CA 83 GLU HA 146.69 . . 0.50 66 1JCAHA 84 GLU CA 84 GLU HA 140.21 . . 0.50 67 1JCAHA 85 PRO CA 85 PRO HA 147.40 . . 0.50 68 1JCAHA 86 LYS CA 86 LYS HA 141.28 . . 0.50 69 1JCAHA 87 ASP CA 87 ASP HA 146.57 . . 0.50 70 1JCAHA 88 GLU CA 88 GLU HA 144.22 . . 0.50 71 1JCAHA 89 ASP CA 89 ASP HA 150.71 . . 0.50 72 1JCAHA 90 PHE CA 90 PHE HA 138.91 . . 0.50 73 1JCAHA 91 SER CA 91 SER HA 139.08 . . 0.50 74 1JCAHA 92 PRO CA 92 PRO HA 153.74 . . 0.50 75 1JCAHA 93 ASP CA 93 ASP HA 133.38 . . 0.50 76 1JCAHA 96 TYR CA 96 TYR HA 144.84 . . 0.50 77 1JCAHA 97 ILE CA 97 ILE HA 132.89 . . 0.50 78 1JCAHA 98 PRO CA 98 PRO HA 142.57 . . 0.50 79 1JCAHA 99 ARG CA 99 ARG HA 142.67 . . 0.50 80 1JCAHA 100 ILE CA 100 ILE HA 142.64 . . 0.50 81 1JCAHA 101 LEU CA 101 LEU HA 139.72 . . 0.50 82 1JCAHA 102 PHE CA 102 PHE HA 143.26 . . 0.50 83 1JCAHA 103 LEU CA 103 LEU HA 143.11 . . 0.50 84 1JCAHA 104 ASP CA 104 ASP HA 126.82 . . 0.50 85 1JCAHA 105 PRO CA 105 PRO HA 148.73 . . 0.50 86 1JCAHA 106 SER CA 106 SER HA 142.19 . . 0.50 87 1JCAHA 108 LYS CA 108 LYS HA 142.10 . . 0.50 88 1JCAHA 109 VAL CA 109 VAL HA 138.60 . . 0.50 89 1JCAHA 110 HIS CA 110 HIS HA 139.58 . . 0.50 90 1JCAHA 111 PRO CA 111 PRO HA 148.01 . . 0.50 91 1JCAHA 112 GLU CA 112 GLU HA 142.68 . . 0.50 92 1JCAHA 113 ILE CA 113 ILE HA 136.70 . . 0.50 93 1JCAHA 114 ILE CA 114 ILE HA 142.95 . . 0.50 94 1JCAHA 115 ASN CA 115 ASN HA 151.20 . . 0.50 95 1JCAHA 116 GLU CA 116 GLU HA 146.53 . . 0.50 96 1JCAHA 117 ASN CA 117 ASN HA 139.47 . . 0.50 97 1JCAHA 119 ASN CA 119 ASN HA 142.32 . . 0.50 98 1JCAHA 120 PRO CA 120 PRO HA 151.66 . . 0.50 99 1JCAHA 121 SER CA 121 SER HA 147.26 . . 0.50 100 1JCAHA 122 TYR CA 122 TYR HA 141.70 . . 0.50 101 1JCAHA 123 LYS CA 123 LYS HA 145.56 . . 0.50 102 1JCAHA 124 TYR CA 124 TYR HA 138.35 . . 0.50 103 1JCAHA 125 PHE CA 125 PHE HA 141.73 . . 0.50 104 1JCAHA 126 TYR CA 126 TYR HA 142.62 . . 0.50 105 1JCAHA 127 VAL CA 127 VAL HA 140.78 . . 0.50 106 1JCAHA 128 SER CA 128 SER HA 140.88 . . 0.50 107 1JCAHA 129 ALA CA 129 ALA HA 141.42 . . 0.50 108 1JCAHA 130 GLU CA 130 GLU HA 147.62 . . 0.50 109 1JCAHA 131 GLN CA 131 GLN HA 143.64 . . 0.50 110 1JCAHA 132 VAL CA 132 VAL HA 144.81 . . 0.50 111 1JCAHA 133 VAL CA 133 VAL HA 147.45 . . 0.50 112 1JCAHA 134 GLN CA 134 GLN HA 148.35 . . 0.50 113 1JCAHA 136 MET CA 136 MET HA 144.25 . . 0.50 114 1JCAHA 137 LYS CA 137 LYS HA 144.31 . . 0.50 115 1JCAHA 138 GLU CA 138 GLU HA 148.45 . . 0.50 116 1JCAHA 139 ALA CA 139 ALA HA 142.32 . . 0.50 117 1JCAHA 140 GLN CA 140 GLN HA 146.58 . . 0.50 118 1JCAHA 141 GLU CA 141 GLU HA 148.08 . . 0.50 119 1JCAHA 142 ARG CA 142 ARG HA 147.33 . . 0.50 120 1JCAHA 143 LEU CA 143 LEU HA 141.91 . . 0.50 121 1JCAHA 144 THR CA 144 THR HA 140.99 . . 0.50 122 1JCAHA 146 ASP CA 146 ASP HA 143.53 . . 0.50 123 1JCAHA 147 ALA CA 147 ALA HA 146.64 . . 0.50 124 1JCAHA 148 PHE CA 148 PHE HA 144.47 . . 0.50 125 1JCAHA 149 ARG CA 149 ARG HA 142.89 . . 0.50 126 1JCAHA 150 LYS CA 150 LYS HA 142.81 . . 0.50 127 1JCAHA 151 LYS CA 151 LYS HA 141.81 . . 0.50 128 1JCAHA 152 HIS CA 152 HIS HA 144.29 . . 0.50 129 1JCAHA 153 LEU CA 153 LEU HA 142.33 . . 0.50 130 1JCAHA 154 GLU CA 154 GLU HA 143.81 . . 0.50 131 1JCAHA 155 ASP CA 155 ASP HA 143.67 . . 0.50 132 1JCAHA 156 GLU CA 156 GLU HA 141.43 . . 0.50 133 1JCAHA 157 LEU CA 157 LEU HA 141.53 . . 0.50 stop_ save_ save_1JCaCb _Saveframe_category coupling_constants _Details 'recorded at 800 and 900 MHz with Ca evolution times of 46 and 44 ms, resp.' loop_ _Experiment_label '3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JCACB 8 MET CA 8 MET CB 32.30 . . 0.50 2 1JCACB 9 SER CA 9 SER CB 36.55 . . 0.50 3 1JCACB 10 ASP CA 10 ASP CB 33.94 . . 0.50 4 1JCACB 13 ASN CA 13 ASN CB 34.99 . . 0.50 5 1JCACB 15 LEU CA 15 LEU CB 35.09 . . 0.50 6 1JCACB 17 LYS CA 17 LYS CB 33.12 . . 0.50 7 1JCACB 19 PHE CA 19 PHE CB 34.05 . . 0.50 8 1JCACB 21 ASP CA 21 ASP CB 35.09 . . 0.50 9 1JCACB 22 HIS CA 22 HIS CB 31.74 . . 0.50 10 1JCACB 23 ILE CA 23 ILE CB 34.33 . . 0.50 11 1JCACB 24 HIS CA 24 HIS CB 37.01 . . 0.50 12 1JCACB 25 TRP CA 25 TRP CB 31.31 . . 0.50 13 1JCACB 26 ARG CA 26 ARG CB 32.00 . . 0.50 14 1JCACB 28 LEU CA 28 LEU CB 33.41 . . 0.50 15 1JCACB 29 GLU CA 29 GLU CB 31.12 . . 0.50 16 1JCACB 30 ASP CA 30 ASP CB 31.62 . . 0.50 17 1JCACB 32 LYS CA 32 LYS CB 31.86 . . 0.50 18 1JCACB 33 LYS CA 33 LYS CB 31.99 . . 0.50 19 1JCACB 34 GLU CA 34 GLU CB 29.15 . . 0.50 20 1JCACB 35 ALA CA 35 ALA CB 31.97 . . 0.50 21 1JCACB 36 ALA CA 36 ALA CB 32.71 . . 0.50 22 1JCACB 37 ALA CA 37 ALA CB 33.08 . . 0.50 23 1JCACB 38 SER CA 38 SER CB 35.97 . . 0.50 24 1JCACB 41 PRO CA 41 PRO CB 30.11 . . 0.50 25 1JCACB 42 LEU CA 42 LEU CB 34.99 . . 0.50 26 1JCACB 43 MET CA 43 MET CB 31.74 . . 0.50 27 1JCACB 44 VAL CA 44 VAL CB 34.02 . . 0.50 28 1JCACB 45 ILE CA 45 ILE CB 34.32 . . 0.50 29 1JCACB 47 HIS CA 47 HIS CB 31.27 . . 0.50 30 1JCACB 48 LYS CA 48 LYS CB 30.31 . . 0.50 31 1JCACB 49 SER CA 49 SER CB 34.92 . . 0.50 32 1JCACB 53 ALA CA 53 ALA CB 32.76 . . 0.50 33 1JCACB 54 CYS CA 54 CYS CB 34.74 . . 0.50 34 1JCACB 55 LYS CA 55 LYS CB 34.13 . . 0.50 35 1JCACB 56 ALA CA 56 ALA CB 33.29 . . 0.50 36 1JCACB 57 LEU CA 57 LEU CB 33.46 . . 0.50 37 1JCACB 59 PRO CA 59 PRO CB 30.52 . . 0.50 38 1JCACB 60 LYS CA 60 LYS CB 33.33 . . 0.50 39 1JCACB 61 PHE CA 61 PHE CB 32.74 . . 0.50 40 1JCACB 62 ALA CA 62 ALA CB 33.46 . . 0.50 41 1JCACB 63 GLU CA 63 GLU CB 31.44 . . 0.50 42 1JCACB 65 THR CA 65 THR CB 33.62 . . 0.50 43 1JCACB 66 GLU CA 66 GLU CB 32.38 . . 0.50 44 1JCACB 67 ILE CA 67 ILE CB 32.59 . . 0.50 45 1JCACB 68 SER CA 68 SER CB 37.41 . . 0.50 46 1JCACB 69 GLU CA 69 GLU CB 30.24 . . 0.50 47 1JCACB 70 LEU CA 70 LEU CB 33.23 . . 0.50 48 1JCACB 71 SER CA 71 SER CB 40.42 . . 0.50 49 1JCACB 72 HIS CA 72 HIS CB 33.34 . . 0.50 50 1JCACB 73 ASN CA 73 ASN CB 35.19 . . 0.50 51 1JCACB 74 PHE CA 74 PHE CB 30.19 . . 0.50 52 1JCACB 75 VAL CA 75 VAL CB 34.64 . . 0.50 53 1JCACB 76 MET CA 76 MET CB 31.21 . . 0.50 54 1JCACB 77 VAL CA 77 VAL CB 33.05 . . 0.50 55 1JCACB 78 ASN CA 78 ASN CB 33.78 . . 0.50 56 1JCACB 79 LEU CA 79 LEU CB 34.52 . . 0.50 57 1JCACB 80 GLU CA 80 GLU CB 31.96 . . 0.50 58 1JCACB 81 ASP CA 81 ASP CB 36.75 . . 0.50 59 1JCACB 82 GLU CA 82 GLU CB 33.66 . . 0.50 60 1JCACB 83 GLU CA 83 GLU CB 30.87 . . 0.50 61 1JCACB 85 PRO CA 85 PRO CB 31.78 . . 0.50 62 1JCACB 86 LYS CA 86 LYS CB 34.23 . . 0.50 63 1JCACB 88 GLU CA 88 GLU CB 31.21 . . 0.50 64 1JCACB 89 ASP CA 89 ASP CB 36.12 . . 0.50 65 1JCACB 90 PHE CA 90 PHE CB 35.08 . . 0.50 66 1JCACB 92 PRO CA 92 PRO CB 28.95 . . 0.50 67 1JCACB 93 ASP CA 93 ASP CB 37.94 . . 0.50 68 1JCACB 96 TYR CA 96 TYR CB 32.28 . . 0.50 69 1JCACB 98 PRO CA 98 PRO CB 28.76 . . 0.50 70 1JCACB 99 ARG CA 99 ARG CB 34.92 . . 0.50 71 1JCACB 100 ILE CA 100 ILE CB 35.52 . . 0.50 72 1JCACB 101 LEU CA 101 LEU CB 34.03 . . 0.50 73 1JCACB 102 PHE CA 102 PHE CB 31.24 . . 0.50 74 1JCACB 103 LEU CA 103 LEU CB 34.67 . . 0.50 75 1JCACB 105 PRO CA 105 PRO CB 29.79 . . 0.50 76 1JCACB 106 SER CA 106 SER CB 37.06 . . 0.50 77 1JCACB 108 LYS CA 108 LYS CB 33.15 . . 0.50 78 1JCACB 109 VAL CA 109 VAL CB 31.86 . . 0.50 79 1JCACB 111 PRO CA 111 PRO CB 28.06 . . 0.50 80 1JCACB 112 GLU CA 112 GLU CB 32.68 . . 0.50 81 1JCACB 113 ILE CA 113 ILE CB 35.64 . . 0.50 82 1JCACB 114 ILE CA 114 ILE CB 32.18 . . 0.50 83 1JCACB 115 ASN CA 115 ASN CB 39.02 . . 0.50 84 1JCACB 116 GLU CA 116 GLU CB 31.05 . . 0.50 85 1JCACB 117 ASN CA 117 ASN CB 35.06 . . 0.50 86 1JCACB 120 PRO CA 120 PRO CB 29.36 . . 0.50 87 1JCACB 121 SER CA 121 SER CB 36.58 . . 0.50 88 1JCACB 122 TYR CA 122 TYR CB 36.93 . . 0.50 89 1JCACB 123 LYS CA 123 LYS CB 32.04 . . 0.50 90 1JCACB 124 TYR CA 124 TYR CB 33.83 . . 0.50 91 1JCACB 125 PHE CA 125 PHE CB 33.81 . . 0.50 92 1JCACB 126 TYR CA 126 TYR CB 32.47 . . 0.50 93 1JCACB 127 VAL CA 127 VAL CB 32.70 . . 0.50 94 1JCACB 128 SER CA 128 SER CB 35.70 . . 0.50 95 1JCACB 129 ALA CA 129 ALA CB 32.22 . . 0.50 96 1JCACB 130 GLU CA 130 GLU CB 30.90 . . 0.50 97 1JCACB 131 GLN CA 131 GLN CB 30.84 . . 0.50 98 1JCACB 132 VAL CA 132 VAL CB 31.28 . . 0.50 99 1JCACB 133 VAL CA 133 VAL CB 31.95 . . 0.50 100 1JCACB 134 GLN CA 134 GLN CB 30.25 . . 0.50 101 1JCACB 136 MET CA 136 MET CB 29.53 . . 0.50 102 1JCACB 137 LYS CA 137 LYS CB 32.11 . . 0.50 103 1JCACB 138 GLU CA 138 GLU CB 31.55 . . 0.50 104 1JCACB 139 ALA CA 139 ALA CB 33.25 . . 0.50 105 1JCACB 140 GLN CA 140 GLN CB 30.42 . . 0.50 106 1JCACB 141 GLU CA 141 GLU CB 31.65 . . 0.50 107 1JCACB 142 ARG CA 142 ARG CB 31.36 . . 0.50 108 1JCACB 143 LEU CA 143 LEU CB 34.91 . . 0.50 109 1JCACB 144 THR CA 144 THR CB 34.10 . . 0.50 110 1JCACB 146 ASP CA 146 ASP CB 33.21 . . 0.50 111 1JCACB 147 ALA CA 147 ALA CB 33.36 . . 0.50 112 1JCACB 148 PHE CA 148 PHE CB 32.46 . . 0.50 113 1JCACB 149 ARG CA 149 ARG CB 31.82 . . 0.50 114 1JCACB 150 LYS CA 150 LYS CB 32.45 . . 0.50 115 1JCACB 151 LYS CA 151 LYS CB 33.36 . . 0.50 116 1JCACB 152 HIS CA 152 HIS CB 34.14 . . 0.50 117 1JCACB 153 LEU CA 153 LEU CB 33.48 . . 0.50 118 1JCACB 154 GLU CA 154 GLU CB 31.38 . . 0.50 119 1JCACB 155 ASP CA 155 ASP CB 33.46 . . 0.50 120 1JCACB 156 GLU CA 156 GLU CB 32.04 . . 0.50 stop_ save_ save_1JCON_ _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '2D IPAP-type HN(a/b-NCO-J)-TROSY' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JC_N_ 7 HIS C 8 MET N -14.71 . . 0.50 2 1JC_N_ 8 MET C 9 SER N -13.97 . . 0.50 3 1JC_N_ 9 SER C 10 ASP N -13.63 . . 0.50 4 1JC_N_ 10 ASP C 11 GLY N -14.88 . . 0.50 5 1JC_N_ 11 GLY C 12 HIS N -14.52 . . 0.50 6 1JC_N_ 13 ASN C 14 GLY N -15.11 . . 0.50 7 1JC_N_ 15 LEU C 16 GLY N -14.19 . . 0.50 8 1JC_N_ 16 GLY C 17 LYS N -14.52 . . 0.50 9 1JC_N_ 17 LYS C 18 GLY N -15.41 . . 0.50 10 1JC_N_ 18 GLY C 19 PHE N -14.88 . . 0.50 11 1JC_N_ 19 PHE C 20 GLY N -14.80 . . 0.50 12 1JC_N_ 20 GLY C 21 ASP N -13.30 . . 0.50 13 1JC_N_ 21 ASP C 22 HIS N -14.37 . . 0.50 14 1JC_N_ 22 HIS C 23 ILE N -16.54 . . 0.50 15 1JC_N_ 23 ILE C 24 HIS N -12.65 . . 0.50 16 1JC_N_ 24 HIS C 25 TRP N -14.52 . . 0.50 17 1JC_N_ 25 TRP C 26 ARG N -14.95 . . 0.50 18 1JC_N_ 26 ARG C 27 THR N -14.22 . . 0.50 19 1JC_N_ 27 THR C 28 LEU N -13.60 . . 0.50 20 1JC_N_ 28 LEU C 29 GLU N -12.83 . . 0.50 21 1JC_N_ 29 GLU C 30 ASP N -13.45 . . 0.50 22 1JC_N_ 30 ASP C 31 GLY N -14.79 . . 0.50 23 1JC_N_ 31 GLY C 32 LYS N -14.57 . . 0.50 24 1JC_N_ 32 LYS C 33 LYS N -14.59 . . 0.50 25 1JC_N_ 34 GLU C 35 ALA N -13.86 . . 0.50 26 1JC_N_ 35 ALA C 36 ALA N -14.70 . . 0.50 27 1JC_N_ 36 ALA C 37 ALA N -14.80 . . 0.50 28 1JC_N_ 37 ALA C 38 SER N -14.73 . . 0.50 29 1JC_N_ 38 SER C 39 GLY N -16.01 . . 0.50 30 1JC_N_ 39 GLY C 40 LEU N -15.92 . . 0.50 31 1JC_N_ 41 PRO C 42 LEU N -13.45 . . 0.50 32 1JC_N_ 42 LEU C 43 MET N -13.04 . . 0.50 33 1JC_N_ 43 MET C 44 VAL N -13.69 . . 0.50 34 1JC_N_ 44 VAL C 45 ILE N -13.55 . . 0.50 35 1JC_N_ 45 ILE C 46 ILE N -13.71 . . 0.50 36 1JC_N_ 46 ILE C 47 HIS N -13.74 . . 0.50 37 1JC_N_ 47 HIS C 48 LYS N -15.17 . . 0.50 38 1JC_N_ 48 LYS C 49 SER N -13.51 . . 0.50 39 1JC_N_ 49 SER C 50 TRP N -14.73 . . 0.50 40 1JC_N_ 53 ALA C 54 CYS N -14.63 . . 0.50 41 1JC_N_ 54 CYS C 55 LYS N -15.01 . . 0.50 42 1JC_N_ 55 LYS C 56 ALA N -14.25 . . 0.50 43 1JC_N_ 56 ALA C 57 LEU N -14.79 . . 0.50 44 1JC_N_ 57 LEU C 58 LYS N -13.43 . . 0.50 45 1JC_N_ 59 PRO C 60 LYS N -13.24 . . 0.50 46 1JC_N_ 60 LYS C 61 PHE N -14.38 . . 0.50 47 1JC_N_ 61 PHE C 62 ALA N -14.03 . . 0.50 48 1JC_N_ 62 ALA C 63 GLU N -14.21 . . 0.50 49 1JC_N_ 63 GLU C 64 SER N -14.83 . . 0.50 50 1JC_N_ 65 THR C 66 GLU N -14.35 . . 0.50 51 1JC_N_ 66 GLU C 67 ILE N -13.51 . . 0.50 52 1JC_N_ 67 ILE C 68 SER N -13.64 . . 0.50 53 1JC_N_ 68 SER C 69 GLU N -15.44 . . 0.50 54 1JC_N_ 69 GLU C 70 LEU N -13.99 . . 0.50 55 1JC_N_ 70 LEU C 71 SER N -12.82 . . 0.50 56 1JC_N_ 71 SER C 72 HIS N -14.64 . . 0.50 57 1JC_N_ 72 HIS C 73 ASN N -15.51 . . 0.50 58 1JC_N_ 73 ASN C 74 PHE N -16.40 . . 0.50 59 1JC_N_ 74 PHE C 75 VAL N -13.90 . . 0.50 60 1JC_N_ 75 VAL C 76 MET N -13.31 . . 0.50 61 1JC_N_ 76 MET C 77 VAL N -13.75 . . 0.50 62 1JC_N_ 77 VAL C 78 ASN N -13.48 . . 0.50 63 1JC_N_ 78 ASN C 79 LEU N -13.91 . . 0.50 64 1JC_N_ 79 LEU C 80 GLU N -12.98 . . 0.50 65 1JC_N_ 80 GLU C 81 ASP N -12.42 . . 0.50 66 1JC_N_ 81 ASP C 82 GLU N -14.44 . . 0.50 67 1JC_N_ 82 GLU C 83 GLU N -14.05 . . 0.50 68 1JC_N_ 83 GLU C 84 GLU N -14.49 . . 0.50 69 1JC_N_ 85 PRO C 86 LYS N -13.36 . . 0.50 70 1JC_N_ 86 LYS C 87 ASP N -14.69 . . 0.50 71 1JC_N_ 87 ASP C 88 GLU N -15.39 . . 0.50 72 1JC_N_ 88 GLU C 89 ASP N -13.72 . . 0.50 73 1JC_N_ 89 ASP C 90 PHE N -13.40 . . 0.50 74 1JC_N_ 90 PHE C 91 SER N -15.55 . . 0.50 75 1JC_N_ 92 PRO C 93 ASP N -14.15 . . 0.50 76 1JC_N_ 93 ASP C 94 GLY N -15.63 . . 0.50 77 1JC_N_ 94 GLY C 95 GLY N -14.81 . . 0.50 78 1JC_N_ 95 GLY C 96 TYR N -15.74 . . 0.50 79 1JC_N_ 96 TYR C 97 ILE N -13.27 . . 0.50 80 1JC_N_ 98 PRO C 99 ARG N -13.47 . . 0.50 81 1JC_N_ 99 ARG C 100 ILE N -14.29 . . 0.50 82 1JC_N_ 100 ILE C 101 LEU N -13.96 . . 0.50 83 1JC_N_ 101 LEU C 102 PHE N -13.69 . . 0.50 84 1JC_N_ 102 PHE C 103 LEU N -14.68 . . 0.50 85 1JC_N_ 103 LEU C 104 ASP N -12.04 . . 0.50 86 1JC_N_ 105 PRO C 106 SER N -13.61 . . 0.50 87 1JC_N_ 106 SER C 107 GLY N -16.08 . . 0.50 88 1JC_N_ 107 GLY C 108 LYS N -15.82 . . 0.50 89 1JC_N_ 108 LYS C 109 VAL N -14.10 . . 0.50 90 1JC_N_ 109 VAL C 110 HIS N -14.30 . . 0.50 91 1JC_N_ 111 PRO C 112 GLU N -15.06 . . 0.50 92 1JC_N_ 112 GLU C 113 ILE N -14.58 . . 0.50 93 1JC_N_ 113 ILE C 114 ILE N -14.56 . . 0.50 94 1JC_N_ 114 ILE C 115 ASN N -13.86 . . 0.50 95 1JC_N_ 115 ASN C 116 GLU N -12.87 . . 0.50 96 1JC_N_ 116 GLU C 117 ASN N -14.04 . . 0.50 97 1JC_N_ 117 ASN C 118 GLY N -16.48 . . 0.50 98 1JC_N_ 118 GLY C 119 ASN N -14.50 . . 0.50 99 1JC_N_ 121 SER C 122 TYR N -15.58 . . 0.50 100 1JC_N_ 122 TYR C 123 LYS N -14.40 . . 0.50 101 1JC_N_ 123 LYS C 124 TYR N -14.56 . . 0.50 102 1JC_N_ 124 TYR C 125 PHE N -15.00 . . 0.50 103 1JC_N_ 125 PHE C 126 TYR N -15.04 . . 0.50 104 1JC_N_ 126 TYR C 127 VAL N -15.38 . . 0.50 105 1JC_N_ 127 VAL C 128 SER N -15.63 . . 0.50 106 1JC_N_ 128 SER C 129 ALA N -14.07 . . 0.50 107 1JC_N_ 129 ALA C 130 GLU N -14.33 . . 0.50 108 1JC_N_ 130 GLU C 131 GLN N -13.54 . . 0.50 109 1JC_N_ 131 GLN C 132 VAL N -13.95 . . 0.50 110 1JC_N_ 132 VAL C 133 VAL N -14.52 . . 0.50 111 1JC_N_ 133 VAL C 134 GLN N -14.18 . . 0.50 112 1JC_N_ 134 GLN C 135 GLY N -14.58 . . 0.50 113 1JC_N_ 136 MET C 137 LYS N -13.81 . . 0.50 114 1JC_N_ 137 LYS C 138 GLU N -14.71 . . 0.50 115 1JC_N_ 138 GLU C 139 ALA N -14.91 . . 0.50 116 1JC_N_ 139 ALA C 140 GLN N -14.28 . . 0.50 117 1JC_N_ 140 GLN C 141 GLU N -14.43 . . 0.50 118 1JC_N_ 141 GLU C 142 ARG N -13.50 . . 0.50 119 1JC_N_ 142 ARG C 143 LEU N -13.85 . . 0.50 120 1JC_N_ 143 LEU C 144 THR N -14.28 . . 0.50 121 1JC_N_ 144 THR C 145 GLY N -15.14 . . 0.50 122 1JC_N_ 145 GLY C 146 ASP N -14.51 . . 0.50 123 1JC_N_ 146 ASP C 147 ALA N -14.10 . . 0.50 124 1JC_N_ 147 ALA C 148 PHE N -15.04 . . 0.50 125 1JC_N_ 148 PHE C 149 ARG N -15.13 . . 0.50 126 1JC_N_ 149 ARG C 150 LYS N -14.47 . . 0.50 127 1JC_N_ 150 LYS C 151 LYS N -14.60 . . 0.50 128 1JC_N_ 151 LYS C 152 HIS N -14.12 . . 0.50 129 1JC_N_ 152 HIS C 153 LEU N -15.04 . . 0.50 130 1JC_N_ 153 LEU C 154 GLU N -13.90 . . 0.50 131 1JC_N_ 154 GLU C 155 ASP N -14.37 . . 0.50 132 1JC_N_ 155 ASP C 156 GLU N -14.84 . . 0.50 133 1JC_N_ 156 GLU C 157 LEU N -15.52 . . 0.50 stop_ save_ save_2JCaN_ _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '2D IPAP-type HN(CO-a/b-NCa-J)-TROSY' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 2JCAN_ 7 HIS CA 8 MET N -7.58 . . 0.50 2 2JCAN_ 8 MET CA 9 SER N -8.34 . . 0.50 3 2JCAN_ 9 SER CA 10 ASP N -6.99 . . 0.50 4 2JCAN_ 10 ASP CA 11 GLY N -7.19 . . 0.50 5 2JCAN_ 12 HIS CA 13 ASN N -6.17 . . 0.50 6 2JCAN_ 13 ASN CA 14 GLY N -7.84 . . 0.50 7 2JCAN_ 14 GLY CA 15 LEU N -6.90 . . 0.50 8 2JCAN_ 15 LEU CA 16 GLY N -6.31 . . 0.50 9 2JCAN_ 16 GLY CA 17 LYS N -6.30 . . 0.50 10 2JCAN_ 17 LYS CA 18 GLY N -6.52 . . 0.50 11 2JCAN_ 18 GLY CA 19 PHE N -6.16 . . 0.50 12 2JCAN_ 19 PHE CA 20 GLY N -7.24 . . 0.50 13 2JCAN_ 20 GLY CA 21 ASP N -9.41 . . 0.50 14 2JCAN_ 21 ASP CA 22 HIS N -6.46 . . 0.50 15 2JCAN_ 22 HIS CA 23 ILE N -5.42 . . 0.50 16 2JCAN_ 23 ILE CA 24 HIS N -8.94 . . 0.50 17 2JCAN_ 24 HIS CA 25 TRP N -7.83 . . 0.50 18 2JCAN_ 25 TRP CA 26 ARG N -8.74 . . 0.50 19 2JCAN_ 26 ARG CA 27 THR N -8.21 . . 0.50 20 2JCAN_ 27 THR CA 28 LEU N -7.59 . . 0.50 21 2JCAN_ 28 LEU CA 29 GLU N -6.34 . . 0.50 22 2JCAN_ 29 GLU CA 30 ASP N -5.77 . . 0.50 23 2JCAN_ 30 ASP CA 31 GLY N -6.30 . . 0.50 24 2JCAN_ 31 GLY CA 32 LYS N -6.84 . . 0.50 25 2JCAN_ 32 LYS CA 33 LYS N -5.77 . . 0.50 26 2JCAN_ 33 LYS CA 34 GLU N -5.88 . . 0.50 27 2JCAN_ 34 GLU CA 35 ALA N -5.48 . . 0.50 28 2JCAN_ 35 ALA CA 36 ALA N -6.43 . . 0.50 29 2JCAN_ 36 ALA CA 37 ALA N -5.90 . . 0.50 30 2JCAN_ 37 ALA CA 38 SER N -6.22 . . 0.50 31 2JCAN_ 38 SER CA 39 GLY N -5.71 . . 0.50 32 2JCAN_ 39 GLY CA 40 LEU N -6.16 . . 0.50 33 2JCAN_ 41 PRO CA 42 LEU N -8.43 . . 0.50 34 2JCAN_ 42 LEU CA 43 MET N -8.77 . . 0.50 35 2JCAN_ 43 MET CA 44 VAL N -8.77 . . 0.50 36 2JCAN_ 44 VAL CA 45 ILE N -8.72 . . 0.50 37 2JCAN_ 45 ILE CA 46 ILE N -7.58 . . 0.50 38 2JCAN_ 46 ILE CA 47 HIS N -7.75 . . 0.50 39 2JCAN_ 47 HIS CA 48 LYS N -9.39 . . 0.50 40 2JCAN_ 48 LYS CA 49 SER N -7.21 . . 0.50 41 2JCAN_ 49 SER CA 50 TRP N -5.04 . . 0.50 42 2JCAN_ 53 ALA CA 54 CYS N -6.26 . . 0.50 43 2JCAN_ 54 CYS CA 55 LYS N -6.51 . . 0.50 44 2JCAN_ 55 LYS CA 56 ALA N -6.14 . . 0.50 45 2JCAN_ 56 ALA CA 57 LEU N -6.11 . . 0.50 46 2JCAN_ 57 LEU CA 58 LYS N -5.54 . . 0.50 47 2JCAN_ 59 PRO CA 60 LYS N -6.29 . . 0.50 48 2JCAN_ 60 LYS CA 61 PHE N -6.48 . . 0.50 49 2JCAN_ 61 PHE CA 62 ALA N -6.29 . . 0.50 50 2JCAN_ 62 ALA CA 63 GLU N -6.47 . . 0.50 51 2JCAN_ 63 GLU CA 64 SER N -5.98 . . 0.50 52 2JCAN_ 65 THR CA 66 GLU N -6.49 . . 0.50 53 2JCAN_ 66 GLU CA 67 ILE N -6.21 . . 0.50 54 2JCAN_ 67 ILE CA 68 SER N -5.74 . . 0.50 55 2JCAN_ 69 GLU CA 70 LEU N -5.03 . . 0.50 56 2JCAN_ 70 LEU CA 71 SER N -5.90 . . 0.50 57 2JCAN_ 71 SER CA 72 HIS N -3.52 . . 0.50 58 2JCAN_ 72 HIS CA 73 ASN N -6.28 . . 0.50 59 2JCAN_ 73 ASN CA 74 PHE N -6.65 . . 0.50 60 2JCAN_ 74 PHE CA 75 VAL N -8.30 . . 0.50 61 2JCAN_ 75 VAL CA 76 MET N -7.56 . . 0.50 62 2JCAN_ 76 MET CA 77 VAL N -9.65 . . 0.50 63 2JCAN_ 77 VAL CA 78 ASN N -8.48 . . 0.50 64 2JCAN_ 78 ASN CA 79 LEU N -9.27 . . 0.50 65 2JCAN_ 79 LEU CA 80 GLU N -8.72 . . 0.50 66 2JCAN_ 80 GLU CA 81 ASP N -7.40 . . 0.50 67 2JCAN_ 81 ASP CA 82 GLU N -7.70 . . 0.50 68 2JCAN_ 82 GLU CA 83 GLU N -5.75 . . 0.50 69 2JCAN_ 83 GLU CA 84 GLU N -6.08 . . 0.50 70 2JCAN_ 85 PRO CA 86 LYS N -8.47 . . 0.50 71 2JCAN_ 86 LYS CA 87 ASP N -6.58 . . 0.50 72 2JCAN_ 87 ASP CA 88 GLU N -9.19 . . 0.50 73 2JCAN_ 88 GLU CA 89 ASP N -5.67 . . 0.50 74 2JCAN_ 89 ASP CA 90 PHE N -6.65 . . 0.50 75 2JCAN_ 90 PHE CA 91 SER N -6.09 . . 0.50 76 2JCAN_ 92 PRO CA 93 ASP N -5.94 . . 0.50 77 2JCAN_ 93 ASP CA 94 GLY N -6.44 . . 0.50 78 2JCAN_ 94 GLY CA 95 GLY N -10.64 . . 0.50 79 2JCAN_ 95 GLY CA 96 TYR N -6.34 . . 0.50 80 2JCAN_ 96 TYR CA 97 ILE N -8.50 . . 0.50 81 2JCAN_ 98 PRO CA 99 ARG N -10.10 . . 0.50 82 2JCAN_ 99 ARG CA 100 ILE N -8.34 . . 0.50 83 2JCAN_ 100 ILE CA 101 LEU N -8.11 . . 0.50 84 2JCAN_ 101 LEU CA 102 PHE N -8.10 . . 0.50 85 2JCAN_ 102 PHE CA 103 LEU N -10.97 . . 0.50 86 2JCAN_ 103 LEU CA 104 ASP N -8.55 . . 0.50 87 2JCAN_ 105 PRO CA 106 SER N -6.13 . . 0.50 88 2JCAN_ 106 SER CA 107 GLY N -5.94 . . 0.50 89 2JCAN_ 107 GLY CA 108 LYS N -6.54 . . 0.50 90 2JCAN_ 108 LYS CA 109 VAL N -7.51 . . 0.50 91 2JCAN_ 109 VAL CA 110 HIS N -7.05 . . 0.50 92 2JCAN_ 111 PRO CA 112 GLU N -5.95 . . 0.50 93 2JCAN_ 112 GLU CA 113 ILE N -5.73 . . 0.50 94 2JCAN_ 113 ILE CA 114 ILE N -6.51 . . 0.50 95 2JCAN_ 114 ILE CA 115 ASN N -8.66 . . 0.50 96 2JCAN_ 115 ASN CA 116 GLU N -7.01 . . 0.50 97 2JCAN_ 116 GLU CA 117 ASN N -6.28 . . 0.50 98 2JCAN_ 117 ASN CA 118 GLY N -6.99 . . 0.50 99 2JCAN_ 118 GLY CA 119 ASN N -9.82 . . 0.50 100 2JCAN_ 120 PRO CA 121 SER N -6.03 . . 0.50 101 2JCAN_ 121 SER CA 122 TYR N -6.00 . . 0.50 102 2JCAN_ 122 TYR CA 123 LYS N -6.77 . . 0.50 103 2JCAN_ 123 LYS CA 124 TYR N -6.49 . . 0.50 104 2JCAN_ 124 TYR CA 125 PHE N -5.73 . . 0.50 105 2JCAN_ 125 PHE CA 126 TYR N -8.36 . . 0.50 106 2JCAN_ 126 TYR CA 127 VAL N -8.53 . . 0.50 107 2JCAN_ 127 VAL CA 128 SER N -5.56 . . 0.50 108 2JCAN_ 128 SER CA 129 ALA N -9.68 . . 0.50 109 2JCAN_ 129 ALA CA 130 GLU N -6.55 . . 0.50 110 2JCAN_ 130 GLU CA 131 GLN N -5.21 . . 0.50 111 2JCAN_ 131 GLN CA 132 VAL N -6.09 . . 0.50 112 2JCAN_ 132 VAL CA 133 VAL N -6.12 . . 0.50 113 2JCAN_ 133 VAL CA 134 GLN N -5.99 . . 0.50 114 2JCAN_ 134 GLN CA 135 GLY N -6.29 . . 0.50 115 2JCAN_ 135 GLY CA 136 MET N -6.79 . . 0.50 116 2JCAN_ 136 MET CA 137 LYS N -6.29 . . 0.50 117 2JCAN_ 137 LYS CA 138 GLU N -5.93 . . 0.50 118 2JCAN_ 138 GLU CA 139 ALA N -6.47 . . 0.50 119 2JCAN_ 139 ALA CA 140 GLN N -6.55 . . 0.50 120 2JCAN_ 140 GLN CA 141 GLU N -6.05 . . 0.50 121 2JCAN_ 141 GLU CA 142 ARG N -6.03 . . 0.50 122 2JCAN_ 142 ARG CA 143 LEU N -5.78 . . 0.50 123 2JCAN_ 143 LEU CA 144 THR N -6.32 . . 0.50 124 2JCAN_ 144 THR CA 145 GLY N -7.54 . . 0.50 125 2JCAN_ 145 GLY CA 146 ASP N -8.14 . . 0.50 126 2JCAN_ 146 ASP CA 147 ALA N -6.13 . . 0.50 127 2JCAN_ 147 ALA CA 148 PHE N -6.37 . . 0.50 128 2JCAN_ 148 PHE CA 149 ARG N -6.22 . . 0.50 129 2JCAN_ 149 ARG CA 150 LYS N -7.65 . . 0.50 130 2JCAN_ 150 LYS CA 151 LYS N -7.65 . . 0.50 131 2JCAN_ 151 LYS CA 152 HIS N -6.33 . . 0.50 132 2JCAN_ 152 HIS CA 153 LEU N -7.12 . . 0.50 133 2JCAN_ 153 LEU CA 154 GLU N -7.30 . . 0.50 134 2JCAN_ 154 GLU CA 155 ASP N -6.63 . . 0.50 135 2JCAN_ 155 ASP CA 156 GLU N -7.40 . . 0.50 136 2JCAN_ 156 GLU CA 157 LEU N -6.55 . . 0.50 stop_ save_ save_2JCOHN _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '2D IPAP-type HN(a/b-NCO-J)-TROSY' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 2JCOHN 7 HIS C 8 MET HN 4.66 . . 0.50 2 2JCOHN 8 MET C 9 SER HN 3.93 . . 0.50 3 2JCOHN 9 SER C 10 ASP HN 5.84 . . 0.50 4 2JCOHN 10 ASP C 11 GLY HN 3.63 . . 0.50 5 2JCOHN 13 ASN C 14 GLY HN 3.84 . . 0.50 6 2JCOHN 15 LEU C 16 GLY HN 4.63 . . 0.50 7 2JCOHN 16 GLY C 17 LYS HN 4.48 . . 0.50 8 2JCOHN 17 LYS C 18 GLY HN 4.38 . . 0.50 9 2JCOHN 18 GLY C 19 PHE HN 3.87 . . 0.50 10 2JCOHN 19 PHE C 20 GLY HN 4.43 . . 0.50 11 2JCOHN 20 GLY C 21 ASP HN 3.16 . . 0.50 12 2JCOHN 21 ASP C 22 HIS HN 4.58 . . 0.50 13 2JCOHN 22 HIS C 23 ILE HN 3.96 . . 0.50 14 2JCOHN 23 ILE C 24 HIS HN 3.54 . . 0.50 15 2JCOHN 24 HIS C 25 TRP HN 4.08 . . 0.50 16 2JCOHN 25 TRP C 26 ARG HN 4.16 . . 0.50 17 2JCOHN 26 ARG C 27 THR HN 3.44 . . 0.50 18 2JCOHN 27 THR C 28 LEU HN 4.09 . . 0.50 19 2JCOHN 28 LEU C 29 GLU HN 3.97 . . 0.50 20 2JCOHN 29 GLU C 30 ASP HN 4.05 . . 0.50 21 2JCOHN 30 ASP C 31 GLY HN 4.94 . . 0.50 22 2JCOHN 31 GLY C 32 LYS HN 5.27 . . 0.50 23 2JCOHN 32 LYS C 33 LYS HN 4.26 . . 0.50 24 2JCOHN 34 GLU C 35 ALA HN 4.13 . . 0.50 25 2JCOHN 35 ALA C 36 ALA HN 4.63 . . 0.50 26 2JCOHN 36 ALA C 37 ALA HN 4.90 . . 0.50 27 2JCOHN 37 ALA C 38 SER HN 3.61 . . 0.50 28 2JCOHN 38 SER C 39 GLY HN 5.04 . . 0.50 29 2JCOHN 39 GLY C 40 LEU HN 3.98 . . 0.50 30 2JCOHN 41 PRO C 42 LEU HN 3.59 . . 0.50 31 2JCOHN 42 LEU C 43 MET HN 4.07 . . 0.50 32 2JCOHN 43 MET C 44 VAL HN 4.42 . . 0.50 33 2JCOHN 44 VAL C 45 ILE HN 4.28 . . 0.50 34 2JCOHN 45 ILE C 46 ILE HN 3.98 . . 0.50 35 2JCOHN 46 ILE C 47 HIS HN 4.52 . . 0.50 36 2JCOHN 47 HIS C 48 LYS HN 4.81 . . 0.50 37 2JCOHN 48 LYS C 49 SER HN 4.55 . . 0.50 38 2JCOHN 49 SER C 50 TRP HN 4.14 . . 0.50 39 2JCOHN 53 ALA C 54 CYS HN 3.91 . . 0.50 40 2JCOHN 54 CYS C 55 LYS HN 3.97 . . 0.50 41 2JCOHN 55 LYS C 56 ALA HN 3.97 . . 0.50 42 2JCOHN 56 ALA C 57 LEU HN 3.98 . . 0.50 43 2JCOHN 57 LEU C 58 LYS HN 3.43 . . 0.50 44 2JCOHN 59 PRO C 60 LYS HN 3.63 . . 0.50 45 2JCOHN 60 LYS C 61 PHE HN 5.11 . . 0.50 46 2JCOHN 61 PHE C 62 ALA HN 3.97 . . 0.50 47 2JCOHN 62 ALA C 63 GLU HN 3.63 . . 0.50 48 2JCOHN 63 GLU C 64 SER HN 3.91 . . 0.50 49 2JCOHN 66 GLU C 67 ILE HN 4.18 . . 0.50 50 2JCOHN 67 ILE C 68 SER HN 3.61 . . 0.50 51 2JCOHN 68 SER C 69 GLU HN 4.94 . . 0.50 52 2JCOHN 69 GLU C 70 LEU HN 4.14 . . 0.50 53 2JCOHN 70 LEU C 71 SER HN 3.09 . . 0.50 54 2JCOHN 71 SER C 72 HIS HN 3.57 . . 0.50 55 2JCOHN 72 HIS C 73 ASN HN 3.94 . . 0.50 56 2JCOHN 73 ASN C 74 PHE HN 4.19 . . 0.50 57 2JCOHN 74 PHE C 75 VAL HN 3.62 . . 0.50 58 2JCOHN 75 VAL C 76 MET HN 4.11 . . 0.50 59 2JCOHN 76 MET C 77 VAL HN 4.36 . . 0.50 60 2JCOHN 77 VAL C 78 ASN HN 4.34 . . 0.50 61 2JCOHN 78 ASN C 79 LEU HN 3.68 . . 0.50 62 2JCOHN 79 LEU C 80 GLU HN 3.95 . . 0.50 63 2JCOHN 80 GLU C 81 ASP HN 3.52 . . 0.50 64 2JCOHN 81 ASP C 82 GLU HN 4.92 . . 0.50 65 2JCOHN 82 GLU C 83 GLU HN 3.96 . . 0.50 66 2JCOHN 83 GLU C 84 GLU HN 4.66 . . 0.50 67 2JCOHN 85 PRO C 86 LYS HN 3.98 . . 0.50 68 2JCOHN 86 LYS C 87 ASP HN 3.89 . . 0.50 69 2JCOHN 88 GLU C 89 ASP HN 4.10 . . 0.50 70 2JCOHN 89 ASP C 90 PHE HN 4.00 . . 0.50 71 2JCOHN 90 PHE C 91 SER HN 4.36 . . 0.50 72 2JCOHN 92 PRO C 93 ASP HN 4.38 . . 0.50 73 2JCOHN 93 ASP C 94 GLY HN 3.76 . . 0.50 74 2JCOHN 94 GLY C 95 GLY HN 3.67 . . 0.50 75 2JCOHN 95 GLY C 96 TYR HN 3.07 . . 0.50 76 2JCOHN 96 TYR C 97 ILE HN 3.37 . . 0.50 77 2JCOHN 99 ARG C 100 ILE HN 4.04 . . 0.50 78 2JCOHN 100 ILE C 101 LEU HN 3.87 . . 0.50 79 2JCOHN 101 LEU C 102 PHE HN 4.10 . . 0.50 80 2JCOHN 102 PHE C 103 LEU HN 4.94 . . 0.50 81 2JCOHN 103 LEU C 104 ASP HN 2.80 . . 0.50 82 2JCOHN 105 PRO C 106 SER HN 3.71 . . 0.50 83 2JCOHN 106 SER C 107 GLY HN 4.67 . . 0.50 84 2JCOHN 107 GLY C 108 LYS HN 4.45 . . 0.50 85 2JCOHN 108 LYS C 109 VAL HN 4.56 . . 0.50 86 2JCOHN 109 VAL C 110 HIS HN 2.36 . . 0.50 87 2JCOHN 111 PRO C 112 GLU HN 5.09 . . 0.50 88 2JCOHN 112 GLU C 113 ILE HN 4.13 . . 0.50 89 2JCOHN 114 ILE C 115 ASN HN 4.10 . . 0.50 90 2JCOHN 115 ASN C 116 GLU HN 3.67 . . 0.50 91 2JCOHN 116 GLU C 117 ASN HN 3.89 . . 0.50 92 2JCOHN 117 ASN C 118 GLY HN 4.45 . . 0.50 93 2JCOHN 118 GLY C 119 ASN HN 3.96 . . 0.50 94 2JCOHN 121 SER C 122 TYR HN 4.17 . . 0.50 95 2JCOHN 122 TYR C 123 LYS HN 4.27 . . 0.50 96 2JCOHN 123 LYS C 124 TYR HN 4.17 . . 0.50 97 2JCOHN 124 TYR C 125 PHE HN 4.07 . . 0.50 98 2JCOHN 125 PHE C 126 TYR HN 2.62 . . 0.50 99 2JCOHN 126 TYR C 127 VAL HN 3.57 . . 0.50 100 2JCOHN 127 VAL C 128 SER HN 4.51 . . 0.50 101 2JCOHN 128 SER C 129 ALA HN 2.79 . . 0.50 102 2JCOHN 129 ALA C 130 GLU HN 4.35 . . 0.50 103 2JCOHN 130 GLU C 131 GLN HN 3.93 . . 0.50 104 2JCOHN 131 GLN C 132 VAL HN 3.89 . . 0.50 105 2JCOHN 132 VAL C 133 VAL HN 5.80 . . 0.50 106 2JCOHN 133 VAL C 134 GLN HN 4.32 . . 0.50 107 2JCOHN 134 GLN C 135 GLY HN 4.35 . . 0.50 108 2JCOHN 136 MET C 137 LYS HN 3.98 . . 0.50 109 2JCOHN 137 LYS C 138 GLU HN 4.18 . . 0.50 110 2JCOHN 138 GLU C 139 ALA HN 3.32 . . 0.50 111 2JCOHN 139 ALA C 140 GLN HN 3.98 . . 0.50 112 2JCOHN 140 GLN C 141 GLU HN 3.87 . . 0.50 113 2JCOHN 141 GLU C 142 ARG HN 3.67 . . 0.50 114 2JCOHN 142 ARG C 143 LEU HN 4.42 . . 0.50 115 2JCOHN 143 LEU C 144 THR HN 3.71 . . 0.50 116 2JCOHN 144 THR C 145 GLY HN 4.58 . . 0.50 117 2JCOHN 145 GLY C 146 ASP HN 3.97 . . 0.50 118 2JCOHN 146 ASP C 147 ALA HN 3.86 . . 0.50 119 2JCOHN 147 ALA C 148 PHE HN 4.04 . . 0.50 120 2JCOHN 148 PHE C 149 ARG HN 4.08 . . 0.50 121 2JCOHN 149 ARG C 150 LYS HN 3.86 . . 0.50 122 2JCOHN 150 LYS C 151 LYS HN 3.66 . . 0.50 123 2JCOHN 151 LYS C 152 HIS HN 3.96 . . 0.50 124 2JCOHN 152 HIS C 153 LEU HN 4.00 . . 0.50 125 2JCOHN 153 LEU C 154 GLU HN 3.75 . . 0.50 126 2JCOHN 154 GLU C 155 ASP HN 3.95 . . 0.50 127 2JCOHN 155 ASP C 156 GLU HN 3.83 . . 0.50 128 2JCOHN 156 GLU C 157 LEU HN 3.86 . . 0.50 stop_ save_ save_2JHaCO _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '3D Ha-coupled [15N,1H]-TROSY-H(N)COCA' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 2JHACO 15 LEU HA 15 LEU C -6.21 . . 0.50 2 2JHACO 19 PHE HA 19 PHE C -5.74 . . 0.50 3 2JHACO 21 ASP HA 21 ASP C -1.46 . . 0.50 4 2JHACO 22 HIS HA 22 HIS C -5.41 . . 0.50 5 2JHACO 24 HIS HA 24 HIS C -2.72 . . 0.50 6 2JHACO 25 TRP HA 25 TRP C -3.33 . . 0.50 7 2JHACO 27 THR HA 27 THR C -0.41 . . 0.50 8 2JHACO 28 LEU HA 28 LEU C -3.68 . . 0.50 9 2JHACO 29 GLU HA 29 GLU C -1.42 . . 0.50 10 2JHACO 30 ASP HA 30 ASP C -3.31 . . 0.50 11 2JHACO 32 LYS HA 32 LYS C -4.66 . . 0.50 12 2JHACO 33 LYS HA 33 LYS C -3.91 . . 0.50 13 2JHACO 34 GLU HA 34 GLU C -4.65 . . 0.50 14 2JHACO 35 ALA HA 35 ALA C -3.79 . . 0.50 15 2JHACO 36 ALA HA 36 ALA C -4.02 . . 0.50 16 2JHACO 37 ALA HA 37 ALA C -4.61 . . 0.50 17 2JHACO 41 PRO HA 41 PRO C -0.16 . . 0.50 18 2JHACO 42 LEU HA 42 LEU C -7.91 . . 0.50 19 2JHACO 43 MET HA 43 MET C -3.12 . . 0.50 20 2JHACO 45 ILE HA 45 ILE C -3.64 . . 0.50 21 2JHACO 46 ILE HA 46 ILE C -3.51 . . 0.50 22 2JHACO 47 HIS HA 47 HIS C -4.58 . . 0.50 23 2JHACO 48 LYS HA 48 LYS C -6.20 . . 0.50 24 2JHACO 49 SER HA 49 SER C -7.40 . . 0.50 25 2JHACO 53 ALA HA 53 ALA C -5.10 . . 0.50 26 2JHACO 54 CYS HA 54 CYS C -2.26 . . 0.50 27 2JHACO 55 LYS HA 55 LYS C -6.49 . . 0.50 28 2JHACO 56 ALA HA 56 ALA C -4.56 . . 0.50 29 2JHACO 57 LEU HA 57 LEU C -0.07 . . 0.50 30 2JHACO 59 PRO HA 59 PRO C -1.24 . . 0.50 31 2JHACO 61 PHE HA 61 PHE C -7.51 . . 0.50 32 2JHACO 62 ALA HA 62 ALA C -2.80 . . 0.50 33 2JHACO 63 GLU HA 63 GLU C -4.11 . . 0.50 34 2JHACO 67 ILE HA 67 ILE C -3.35 . . 0.50 35 2JHACO 68 SER HA 68 SER C -1.37 . . 0.50 36 2JHACO 69 GLU HA 69 GLU C -5.79 . . 0.50 37 2JHACO 70 LEU HA 70 LEU C -6.32 . . 0.50 38 2JHACO 71 SER HA 71 SER C -3.16 . . 0.50 39 2JHACO 73 ASN HA 73 ASN C -5.03 . . 0.50 40 2JHACO 74 PHE HA 74 PHE C -4.25 . . 0.50 41 2JHACO 76 MET HA 76 MET C -2.99 . . 0.50 42 2JHACO 77 VAL HA 77 VAL C -4.02 . . 0.50 43 2JHACO 78 ASN HA 78 ASN C 0.26 . . 0.50 44 2JHACO 79 LEU HA 79 LEU C -3.83 . . 0.50 45 2JHACO 80 GLU HA 80 GLU C -3.61 . . 0.50 46 2JHACO 83 GLU HA 83 GLU C -4.73 . . 0.50 47 2JHACO 85 PRO HA 85 PRO C -0.58 . . 0.50 48 2JHACO 86 LYS HA 86 LYS C -4.92 . . 0.50 49 2JHACO 88 GLU HA 88 GLU C -5.51 . . 0.50 50 2JHACO 89 ASP HA 89 ASP C -0.52 . . 0.50 51 2JHACO 93 ASP HA 93 ASP C -6.07 . . 0.50 52 2JHACO 100 ILE HA 100 ILE C -0.81 . . 0.50 53 2JHACO 101 LEU HA 101 LEU C -6.52 . . 0.50 54 2JHACO 102 PHE HA 102 PHE C -1.99 . . 0.50 55 2JHACO 105 PRO HA 105 PRO C -2.60 . . 0.50 56 2JHACO 106 SER HA 106 SER C -5.49 . . 0.50 57 2JHACO 108 LYS HA 108 LYS C 0.50 . . 0.50 58 2JHACO 109 VAL HA 109 VAL C -3.11 . . 0.50 59 2JHACO 111 PRO HA 111 PRO C -3.54 . . 0.50 60 2JHACO 112 GLU HA 112 GLU C -4.13 . . 0.50 61 2JHACO 114 ILE HA 114 ILE C -2.91 . . 0.50 62 2JHACO 116 GLU HA 116 GLU C -3.71 . . 0.50 63 2JHACO 117 ASN HA 117 ASN C -6.27 . . 0.50 64 2JHACO 120 PRO HA 120 PRO C -1.17 . . 0.50 65 2JHACO 121 SER HA 121 SER C -5.13 . . 0.50 66 2JHACO 122 TYR HA 122 TYR C -2.63 . . 0.50 67 2JHACO 123 LYS HA 123 LYS C -5.67 . . 0.50 68 2JHACO 124 TYR HA 124 TYR C -2.69 . . 0.50 69 2JHACO 126 TYR HA 126 TYR C -5.46 . . 0.50 70 2JHACO 127 VAL HA 127 VAL C -5.51 . . 0.50 71 2JHACO 128 SER HA 128 SER C -5.67 . . 0.50 72 2JHACO 129 ALA HA 129 ALA C -5.20 . . 0.50 73 2JHACO 130 GLU HA 130 GLU C -4.98 . . 0.50 74 2JHACO 131 GLN HA 131 GLN C -1.17 . . 0.50 75 2JHACO 132 VAL HA 132 VAL C -3.55 . . 0.50 76 2JHACO 133 VAL HA 133 VAL C -4.19 . . 0.50 77 2JHACO 134 GLN HA 134 GLN C -3.05 . . 0.50 78 2JHACO 136 MET HA 136 MET C -5.64 . . 0.50 79 2JHACO 137 LYS HA 137 LYS C -5.47 . . 0.50 80 2JHACO 138 GLU HA 138 GLU C -5.07 . . 0.50 81 2JHACO 139 ALA HA 139 ALA C -3.93 . . 0.50 82 2JHACO 140 GLN HA 140 GLN C -4.49 . . 0.50 83 2JHACO 141 GLU HA 141 GLU C -4.16 . . 0.50 84 2JHACO 143 LEU HA 143 LEU C -5.14 . . 0.50 85 2JHACO 144 THR HA 144 THR C -4.86 . . 0.50 86 2JHACO 146 ASP HA 146 ASP C -5.00 . . 0.50 87 2JHACO 147 ALA HA 147 ALA C -4.63 . . 0.50 88 2JHACO 148 PHE HA 148 PHE C -4.76 . . 0.50 89 2JHACO 149 ARG HA 149 ARG C -4.69 . . 0.50 90 2JHACO 150 LYS HA 150 LYS C -2.41 . . 0.50 91 2JHACO 152 HIS HA 152 HIS C -4.18 . . 0.50 92 2JHACO 153 LEU HA 153 LEU C -4.52 . . 0.50 93 2JHACO 154 GLU HA 154 GLU C -4.88 . . 0.50 94 2JHACO 155 ASP HA 155 ASP C -4.88 . . 0.50 95 2JHACO 156 GLU HA 156 GLU C -4.45 . . 0.50 stop_ save_ save_2JHNCa _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '2D IPAP-type HN(CO-a/b-NCa-J)-TROSY' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 2JHNCA 8 MET HN 8 MET CA 2.52 . . 0.50 2 2JHNCA 9 SER HN 9 SER CA 1.03 . . 0.50 3 2JHNCA 10 ASP HN 10 ASP CA 2.81 . . 0.50 4 2JHNCA 11 GLY HN 11 GLY CA 3.35 . . 0.50 5 2JHNCA 13 ASN HN 13 ASN CA 1.15 . . 0.50 6 2JHNCA 14 GLY HN 14 GLY CA 2.48 . . 0.50 7 2JHNCA 15 LEU HN 15 LEU CA 1.06 . . 0.50 8 2JHNCA 16 GLY HN 16 GLY CA 1.32 . . 0.50 9 2JHNCA 17 LYS HN 17 LYS CA 1.93 . . 0.50 10 2JHNCA 18 GLY HN 18 GLY CA 3.86 . . 0.50 11 2JHNCA 19 PHE HN 19 PHE CA 2.17 . . 0.50 12 2JHNCA 20 GLY HN 20 GLY CA 1.05 . . 0.50 13 2JHNCA 21 ASP HN 21 ASP CA 1.15 . . 0.50 14 2JHNCA 22 HIS HN 22 HIS CA 0.38 . . 0.50 15 2JHNCA 23 ILE HN 23 ILE CA -0.05 . . 0.50 16 2JHNCA 24 HIS HN 24 HIS CA 5.18 . . 0.50 17 2JHNCA 25 TRP HN 25 TRP CA 2.31 . . 0.50 18 2JHNCA 26 ARG HN 26 ARG CA 1.81 . . 0.50 19 2JHNCA 27 THR HN 27 THR CA 1.24 . . 0.50 20 2JHNCA 28 LEU HN 28 LEU CA 1.43 . . 0.50 21 2JHNCA 29 GLU HN 29 GLU CA 2.96 . . 0.50 22 2JHNCA 30 ASP HN 30 ASP CA 2.38 . . 0.50 23 2JHNCA 31 GLY HN 31 GLY CA 0.66 . . 0.50 24 2JHNCA 32 LYS HN 32 LYS CA 4.54 . . 0.50 25 2JHNCA 33 LYS HN 33 LYS CA 2.69 . . 0.50 26 2JHNCA 34 GLU HN 34 GLU CA 2.98 . . 0.50 27 2JHNCA 35 ALA HN 35 ALA CA 2.48 . . 0.50 28 2JHNCA 36 ALA HN 36 ALA CA 2.33 . . 0.50 29 2JHNCA 37 ALA HN 37 ALA CA 2.29 . . 0.50 30 2JHNCA 38 SER HN 38 SER CA 1.95 . . 0.50 31 2JHNCA 39 GLY HN 39 GLY CA 1.41 . . 0.50 32 2JHNCA 40 LEU HN 40 LEU CA 1.48 . . 0.50 33 2JHNCA 42 LEU HN 42 LEU CA 1.29 . . 0.50 34 2JHNCA 43 MET HN 43 MET CA 2.41 . . 0.50 35 2JHNCA 44 VAL HN 44 VAL CA 0.96 . . 0.50 36 2JHNCA 45 ILE HN 45 ILE CA 1.00 . . 0.50 37 2JHNCA 46 ILE HN 46 ILE CA 1.87 . . 0.50 38 2JHNCA 47 HIS HN 47 HIS CA 1.91 . . 0.50 39 2JHNCA 48 LYS HN 48 LYS CA 0.80 . . 0.50 40 2JHNCA 49 SER HN 49 SER CA 2.12 . . 0.50 41 2JHNCA 50 TRP HN 50 TRP CA 3.10 . . 0.50 42 2JHNCA 54 CYS HN 54 CYS CA 1.09 . . 0.50 43 2JHNCA 55 LYS HN 55 LYS CA 1.91 . . 0.50 44 2JHNCA 56 ALA HN 56 ALA CA 1.88 . . 0.50 45 2JHNCA 57 LEU HN 57 LEU CA 2.16 . . 0.50 46 2JHNCA 58 LYS HN 58 LYS CA 2.01 . . 0.50 47 2JHNCA 60 LYS HN 60 LYS CA 0.98 . . 0.50 48 2JHNCA 61 PHE HN 61 PHE CA 2.82 . . 0.50 49 2JHNCA 62 ALA HN 62 ALA CA 2.08 . . 0.50 50 2JHNCA 63 GLU HN 63 GLU CA 1.62 . . 0.50 51 2JHNCA 64 SER HN 64 SER CA 3.23 . . 0.50 52 2JHNCA 66 GLU HN 66 GLU CA 2.90 . . 0.50 53 2JHNCA 67 ILE HN 67 ILE CA 1.11 . . 0.50 54 2JHNCA 68 SER HN 68 SER CA 3.35 . . 0.50 55 2JHNCA 70 LEU HN 70 LEU CA -0.22 . . 0.50 56 2JHNCA 71 SER HN 71 SER CA 2.73 . . 0.50 57 2JHNCA 72 HIS HN 72 HIS CA 1.83 . . 0.50 58 2JHNCA 73 ASN HN 73 ASN CA 2.34 . . 0.50 59 2JHNCA 74 PHE HN 74 PHE CA 0.64 . . 0.50 60 2JHNCA 75 VAL HN 75 VAL CA 2.36 . . 0.50 61 2JHNCA 76 MET HN 76 MET CA 1.68 . . 0.50 62 2JHNCA 77 VAL HN 77 VAL CA 1.31 . . 0.50 63 2JHNCA 78 ASN HN 78 ASN CA 0.56 . . 0.50 64 2JHNCA 79 LEU HN 79 LEU CA 1.01 . . 0.50 65 2JHNCA 80 GLU HN 80 GLU CA 1.51 . . 0.50 66 2JHNCA 81 ASP HN 81 ASP CA 0.93 . . 0.50 67 2JHNCA 82 GLU HN 82 GLU CA 1.23 . . 0.50 68 2JHNCA 83 GLU HN 83 GLU CA 1.89 . . 0.50 69 2JHNCA 84 GLU HN 84 GLU CA 3.12 . . 0.50 70 2JHNCA 86 LYS HN 86 LYS CA 2.47 . . 0.50 71 2JHNCA 87 ASP HN 87 ASP CA 1.97 . . 0.50 72 2JHNCA 89 ASP HN 89 ASP CA 2.44 . . 0.50 73 2JHNCA 90 PHE HN 90 PHE CA 1.00 . . 0.50 74 2JHNCA 91 SER HN 91 SER CA 2.39 . . 0.50 75 2JHNCA 93 ASP HN 93 ASP CA 0.81 . . 0.50 76 2JHNCA 94 GLY HN 94 GLY CA 1.80 . . 0.50 77 2JHNCA 95 GLY HN 95 GLY CA 2.23 . . 0.50 78 2JHNCA 96 TYR HN 96 TYR CA 1.30 . . 0.50 79 2JHNCA 100 ILE HN 100 ILE CA 1.99 . . 0.50 80 2JHNCA 101 LEU HN 101 LEU CA 1.02 . . 0.50 81 2JHNCA 102 PHE HN 102 PHE CA -1.17 . . 0.50 82 2JHNCA 103 LEU HN 103 LEU CA 2.75 . . 0.50 83 2JHNCA 104 ASP HN 104 ASP CA 3.55 . . 0.50 84 2JHNCA 106 SER HN 106 SER CA 1.42 . . 0.50 85 2JHNCA 107 GLY HN 107 GLY CA 0.93 . . 0.50 86 2JHNCA 108 LYS HN 108 LYS CA 1.22 . . 0.50 87 2JHNCA 109 VAL HN 109 VAL CA 4.09 . . 0.50 88 2JHNCA 110 HIS HN 110 HIS CA 1.51 . . 0.50 89 2JHNCA 112 GLU HN 112 GLU CA 1.34 . . 0.50 90 2JHNCA 113 ILE HN 113 ILE CA 2.83 . . 0.50 91 2JHNCA 114 ILE HN 114 ILE CA 1.29 . . 0.50 92 2JHNCA 115 ASN HN 115 ASN CA 1.51 . . 0.50 93 2JHNCA 116 GLU HN 116 GLU CA 2.79 . . 0.50 94 2JHNCA 117 ASN HN 117 ASN CA 1.08 . . 0.50 95 2JHNCA 118 GLY HN 118 GLY CA 1.51 . . 0.50 96 2JHNCA 119 ASN HN 119 ASN CA 1.99 . . 0.50 97 2JHNCA 122 TYR HN 122 TYR CA 1.58 . . 0.50 98 2JHNCA 123 LYS HN 123 LYS CA 2.05 . . 0.50 99 2JHNCA 124 TYR HN 124 TYR CA 2.40 . . 0.50 100 2JHNCA 125 PHE HN 125 PHE CA 1.86 . . 0.50 101 2JHNCA 126 TYR HN 126 TYR CA 3.54 . . 0.50 102 2JHNCA 127 VAL HN 127 VAL CA 2.34 . . 0.50 103 2JHNCA 128 SER HN 128 SER CA 1.12 . . 0.50 104 2JHNCA 129 ALA HN 129 ALA CA 1.83 . . 0.50 105 2JHNCA 130 GLU HN 130 GLU CA 3.68 . . 0.50 106 2JHNCA 131 GLN HN 131 GLN CA 1.76 . . 0.50 107 2JHNCA 132 VAL HN 132 VAL CA 2.55 . . 0.50 108 2JHNCA 133 VAL HN 133 VAL CA 1.74 . . 0.50 109 2JHNCA 134 GLN HN 134 GLN CA 2.18 . . 0.50 110 2JHNCA 135 GLY HN 135 GLY CA 1.57 . . 0.50 111 2JHNCA 136 MET HN 136 MET CA 2.85 . . 0.50 112 2JHNCA 137 LYS HN 137 LYS CA 2.87 . . 0.50 113 2JHNCA 138 GLU HN 138 GLU CA 1.85 . . 0.50 114 2JHNCA 139 ALA HN 139 ALA CA 1.48 . . 0.50 115 2JHNCA 140 GLN HN 140 GLN CA 3.18 . . 0.50 116 2JHNCA 141 GLU HN 141 GLU CA 1.89 . . 0.50 117 2JHNCA 142 ARG HN 142 ARG CA 1.08 . . 0.50 118 2JHNCA 144 THR HN 144 THR CA 1.87 . . 0.50 119 2JHNCA 145 GLY HN 145 GLY CA 1.95 . . 0.50 120 2JHNCA 146 ASP HN 146 ASP CA 2.99 . . 0.50 121 2JHNCA 147 ALA HN 147 ALA CA 2.29 . . 0.50 122 2JHNCA 148 PHE HN 148 PHE CA 2.16 . . 0.50 123 2JHNCA 149 ARG HN 149 ARG CA 1.92 . . 0.50 124 2JHNCA 150 LYS HN 150 LYS CA 2.04 . . 0.50 125 2JHNCA 151 LYS HN 151 LYS CA 1.64 . . 0.50 126 2JHNCA 152 HIS HN 152 HIS CA 0.54 . . 0.50 127 2JHNCA 153 LEU HN 153 LEU CA 2.03 . . 0.50 128 2JHNCA 154 GLU HN 154 GLU CA 1.93 . . 0.50 129 2JHNCA 155 ASP HN 155 ASP CA 1.97 . . 0.50 130 2JHNCA 156 GLU HN 156 GLU CA 1.92 . . 0.50 131 2JHNCA 157 LEU HN 157 LEU CA 1.62 . . 0.50 stop_ save_ save_1JN_HN _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '2D [15N,1H]-IPAP-HSQC' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H . _Mol_system_component_name ERp1 _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JN_HN 11 GLY N 11 GLY HN -94.03 . . 0.50 2 1JN_HN 14 GLY N 14 GLY HN -94.21 . . 0.50 3 1JN_HN 16 GLY N 16 GLY HN -94.55 . . 0.50 4 1JN_HN 17 LYS N 17 LYS HN -93.16 . . 0.50 5 1JN_HN 18 GLY N 18 GLY HN -92.74 . . 0.50 6 1JN_HN 20 GLY N 20 GLY HN -93.78 . . 0.50 7 1JN_HN 21 ASP N 21 ASP HN -92.64 . . 0.50 8 1JN_HN 23 ILE N 23 ILE HN -92.68 . . 0.50 9 1JN_HN 24 HIS N 24 HIS HN -92.45 . . 0.50 10 1JN_HN 25 TRP N 25 TRP HN -92.96 . . 0.50 11 1JN_HN 26 ARG N 26 ARG HN -93.38 . . 0.50 12 1JN_HN 27 THR N 27 THR HN -93.10 . . 0.50 13 1JN_HN 28 LEU N 28 LEU HN -94.01 . . 0.50 14 1JN_HN 29 GLU N 29 GLU HN -93.42 . . 0.50 15 1JN_HN 30 ASP N 30 ASP HN -97.95 . . 0.50 16 1JN_HN 31 GLY N 31 GLY HN -95.12 . . 0.50 17 1JN_HN 32 LYS N 32 LYS HN -94.32 . . 0.50 18 1JN_HN 33 LYS N 33 LYS HN -94.56 . . 0.50 19 1JN_HN 35 ALA N 35 ALA HN -93.59 . . 0.50 20 1JN_HN 36 ALA N 36 ALA HN -94.34 . . 0.50 21 1JN_HN 37 ALA N 37 ALA HN -94.67 . . 0.50 22 1JN_HN 38 SER N 38 SER HN -92.99 . . 0.50 23 1JN_HN 39 GLY N 39 GLY HN -94.14 . . 0.50 24 1JN_HN 40 LEU N 40 LEU HN -93.56 . . 0.50 25 1JN_HN 42 LEU N 42 LEU HN -91.30 . . 0.50 26 1JN_HN 43 MET N 43 MET HN -92.31 . . 0.50 27 1JN_HN 44 VAL N 44 VAL HN -93.07 . . 0.50 28 1JN_HN 45 ILE N 45 ILE HN -92.02 . . 0.50 29 1JN_HN 46 ILE N 46 ILE HN -92.80 . . 0.50 30 1JN_HN 47 HIS N 47 HIS HN -93.66 . . 0.50 31 1JN_HN 48 LYS N 48 LYS HN -90.45 . . 0.50 32 1JN_HN 49 SER N 49 SER HN -91.99 . . 0.50 33 1JN_HN 50 TRP N 50 TRP HN -91.40 . . 0.50 34 1JN_HN 54 CYS N 54 CYS HN -92.86 . . 0.50 35 1JN_HN 55 LYS N 55 LYS HN -94.50 . . 0.50 36 1JN_HN 56 ALA N 56 ALA HN -92.12 . . 0.50 37 1JN_HN 57 LEU N 57 LEU HN -93.39 . . 0.50 38 1JN_HN 58 LYS N 58 LYS HN -93.47 . . 0.50 39 1JN_HN 60 LYS N 60 LYS HN -92.19 . . 0.50 40 1JN_HN 61 PHE N 61 PHE HN -93.81 . . 0.50 41 1JN_HN 62 ALA N 62 ALA HN -93.25 . . 0.50 42 1JN_HN 63 GLU N 63 GLU HN -96.27 . . 0.50 43 1JN_HN 64 SER N 64 SER HN -94.75 . . 0.50 44 1JN_HN 67 ILE N 67 ILE HN -93.63 . . 0.50 45 1JN_HN 68 SER N 68 SER HN -92.49 . . 0.50 46 1JN_HN 70 LEU N 70 LEU HN -92.30 . . 0.50 47 1JN_HN 72 HIS N 72 HIS HN -94.10 . . 0.50 48 1JN_HN 73 ASN N 73 ASN HN -92.07 . . 0.50 49 1JN_HN 74 PHE N 74 PHE HN -93.39 . . 0.50 50 1JN_HN 75 VAL N 75 VAL HN -92.15 . . 0.50 51 1JN_HN 76 MET N 76 MET HN -92.51 . . 0.50 52 1JN_HN 77 VAL N 77 VAL HN -93.78 . . 0.50 53 1JN_HN 78 ASN N 78 ASN HN -93.62 . . 0.50 54 1JN_HN 79 LEU N 79 LEU HN -92.78 . . 0.50 55 1JN_HN 80 GLU N 80 GLU HN -92.46 . . 0.50 56 1JN_HN 81 ASP N 81 ASP HN -93.37 . . 0.50 57 1JN_HN 82 GLU N 82 GLU HN -93.85 . . 0.50 58 1JN_HN 83 GLU N 83 GLU HN -93.39 . . 0.50 59 1JN_HN 84 GLU N 84 GLU HN -94.33 . . 0.50 60 1JN_HN 86 LYS N 86 LYS HN -92.79 . . 0.50 61 1JN_HN 87 ASP N 87 ASP HN -93.36 . . 0.50 62 1JN_HN 88 GLU N 88 GLU HN -94.60 . . 0.50 63 1JN_HN 89 ASP N 89 ASP HN -93.62 . . 0.50 64 1JN_HN 90 PHE N 90 PHE HN -92.40 . . 0.50 65 1JN_HN 91 SER N 91 SER HN -93.40 . . 0.50 66 1JN_HN 93 ASP N 93 ASP HN -93.10 . . 0.50 67 1JN_HN 94 GLY N 94 GLY HN -93.31 . . 0.50 68 1JN_HN 95 GLY N 95 GLY HN -92.86 . . 0.50 69 1JN_HN 96 TYR N 96 TYR HN -91.61 . . 0.50 70 1JN_HN 97 ILE N 97 ILE HN -91.05 . . 0.50 71 1JN_HN 99 ARG N 99 ARG HN -92.80 . . 0.50 72 1JN_HN 100 ILE N 100 ILE HN -92.86 . . 0.50 73 1JN_HN 101 LEU N 101 LEU HN -95.06 . . 0.50 74 1JN_HN 102 PHE N 102 PHE HN -93.26 . . 0.50 75 1JN_HN 103 LEU N 103 LEU HN -94.04 . . 0.50 76 1JN_HN 104 ASP N 104 ASP HN -92.87 . . 0.50 77 1JN_HN 106 SER N 106 SER HN -91.87 . . 0.50 78 1JN_HN 107 GLY N 107 GLY HN -93.67 . . 0.50 79 1JN_HN 108 LYS N 108 LYS HN -94.06 . . 0.50 80 1JN_HN 112 GLU N 112 GLU HN -92.82 . . 0.50 81 1JN_HN 113 ILE N 113 ILE HN -92.87 . . 0.50 82 1JN_HN 114 ILE N 114 ILE HN -93.98 . . 0.50 83 1JN_HN 115 ASN N 115 ASN HN -95.12 . . 0.50 84 1JN_HN 116 GLU N 116 GLU HN -94.80 . . 0.50 85 1JN_HN 117 ASN N 117 ASN HN -92.63 . . 0.50 86 1JN_HN 118 GLY N 118 GLY HN -93.67 . . 0.50 87 1JN_HN 119 ASN N 119 ASN HN -92.72 . . 0.50 88 1JN_HN 122 TYR N 122 TYR HN -93.02 . . 0.50 89 1JN_HN 123 LYS N 123 LYS HN -93.83 . . 0.50 90 1JN_HN 124 TYR N 124 TYR HN -92.27 . . 0.50 91 1JN_HN 125 PHE N 125 PHE HN -93.27 . . 0.50 92 1JN_HN 126 TYR N 126 TYR HN -92.36 . . 0.50 93 1JN_HN 128 SER N 128 SER HN -93.83 . . 0.50 94 1JN_HN 129 ALA N 129 ALA HN -93.10 . . 0.50 95 1JN_HN 130 GLU N 130 GLU HN -92.36 . . 0.50 96 1JN_HN 131 GLN N 131 GLN HN -92.84 . . 0.50 97 1JN_HN 132 VAL N 132 VAL HN -92.74 . . 0.50 98 1JN_HN 134 GLN N 134 GLN HN -93.52 . . 0.50 99 1JN_HN 135 GLY N 135 GLY HN -94.27 . . 0.50 100 1JN_HN 138 GLU N 138 GLU HN -93.25 . . 0.50 101 1JN_HN 139 ALA N 139 ALA HN -96.03 . . 0.50 102 1JN_HN 140 GLN N 140 GLN HN -92.83 . . 0.50 103 1JN_HN 142 ARG N 142 ARG HN -93.41 . . 0.50 104 1JN_HN 143 LEU N 143 LEU HN -93.70 . . 0.50 105 1JN_HN 144 THR N 144 THR HN -93.06 . . 0.50 106 1JN_HN 145 GLY N 145 GLY HN -94.10 . . 0.50 107 1JN_HN 146 ASP N 146 ASP HN -93.59 . . 0.50 108 1JN_HN 147 ALA N 147 ALA HN -93.91 . . 0.50 109 1JN_HN 148 PHE N 148 PHE HN -93.10 . . 0.50 110 1JN_HN 149 ARG N 149 ARG HN -93.33 . . 0.50 111 1JN_HN 150 LYS N 150 LYS HN -93.17 . . 0.50 112 1JN_HN 151 LYS N 151 LYS HN -92.45 . . 0.50 113 1JN_HN 153 LEU N 153 LEU HN -93.64 . . 0.50 114 1JN_HN 154 GLU N 154 GLU HN -92.84 . . 0.50 115 1JN_HN 155 ASP N 155 ASP HN -93.57 . . 0.50 116 1JN_HN 156 GLU N 156 GLU HN -92.87 . . 0.50 117 1JN_HN 157 LEU N 157 LEU HN -92.34 . . 0.50 stop_ save_