data_16546 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 16546 _Entry.Title ; Backbone 1H and 15N Chemical Shifts for Disordered A30P alpha-Synuclein ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2009-10-07 _Entry.Accession_date 2009-10-07 _Entry.Last_release_date . _Entry.Original_release_date . _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details '1H/15N chemical shift assignments for disordered A30P alpha-synuclein at pH 6.0' _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Christina Bodner . R. . 16546 2 Christopher Dobson . M. . 16546 3 Ad Bax . . . 16546 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 16546 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 132 16546 '1H chemical shifts' 132 16546 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 3 . . 2010-04-29 2009-10-07 update BMRB 'update related entries' 16546 2 . . 2010-02-23 2009-10-07 update BMRB 'completed entry citation' 16546 1 . . 2010-01-28 2009-10-07 original author 'original release' 16546 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 16300 aSyn 16546 BMRB 16342 aSyn-pH3 16546 BMRB 16543 '1H/15N chemical shift assignments for disordered alpha-synuclein at pH 6.0' 16546 BMRB 16547 '1H/15N chemical shift assignments for disordered E46K alpha-synuclein at pH 6.0' 16546 BMRB 16548 '1H/15N chemical shift assignments for disordered A53T alpha-synuclein at pH 6.0' 16546 BMRB 16904 alpha-synuclei 16546 BMRB 6968 aSyn-protonless 16546 BMRB 7244 gSyn 16546 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 16546 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 20041693 _Citation.Full_citation . _Citation.Title 'Differential phospholipid binding of alpha-synuclein variants implicated in Parkinson's disease revealed by solution NMR spectroscopy.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev Biochemistry _Citation.Journal_name_full Biochemistry _Citation.Journal_volume 49 _Citation.Journal_issue 5 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 862 _Citation.Page_last 871 _Citation.Year 2010 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Christina Bodner . R. . 16546 1 2 Alexander Maltsev . S. . 16546 1 3 Christopher Dobson . M. . 16546 1 4 Ad Bax . . . 16546 1 stop_ loop_ _Citation_keyword.Keyword _Citation_keyword.Entry_ID _Citation_keyword.Citation_ID alpha-helix 16546 1 'membrane binding' 16546 1 NOE 16546 1 relaxation 16546 1 SUV 16546 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 16546 _Assembly.ID 1 _Assembly.Name 'monomer A30P alpha-synuclein' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'monomer A30P alpha-synuclein' 1 $A30P_alpha-synuclein A . yes native no no . . . 16546 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_A30P_alpha-synuclein _Entity.Sf_category entity _Entity.Sf_framecode A30P_alpha-synuclein _Entity.Entry_ID 16546 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name A30P_alpha-synuclein _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MDVFMKGLSKAKEGVVAAAE KTKQGVAEAPGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 140 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation 'A30P disease variant' _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 16300 . alpha-synuclein . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 2 no BMRB 16302 . alpha-synuclein . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 3 no BMRB 16342 . human_a-synuclein . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 4 no BMRB 16543 . "monomer alpha-synuclein" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 5 no BMRB 16547 . E46K_alpha-synuclein . . . . . 100.00 140 98.57 99.29 5.19e-88 . . . . 16546 1 6 no BMRB 16548 . A53T_alpha-synuclein . . . . . 100.00 140 98.57 98.57 3.39e-88 . . . . 16546 1 7 no BMRB 16904 . alpha-synuclein . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 8 no BMRB 16939 . WT_alpha-synuclein_fibrils . . . . . 100.00 140 98.57 98.57 2.29e-87 . . . . 16546 1 9 no BMRB 17214 . A30P_alpha-synuclein . . . . . 100.00 140 100.00 100.00 8.85e-90 . . . . 16546 1 10 no BMRB 17498 . alpha-synuclein . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 11 no BMRB 17648 . A30P_alpha-synuclein . . . . . 100.00 140 99.29 99.29 1.39e-88 . . . . 16546 1 12 no BMRB 17649 . A53T_alpha-synuclein_fibrils . . . . . 100.00 140 97.86 97.86 7.87e-87 . . . . 16546 1 13 no BMRB 17654 . E46K_alpha-synuclein_fibrils . . . . . 100.00 140 97.86 98.57 6.54e-87 . . . . 16546 1 14 no BMRB 17665 . aSyn . . . . . 100.00 150 99.29 99.29 4.82e-88 . . . . 16546 1 15 no BMRB 17910 . wild-type_alpha-synuclein_formed_in_phospholipid_vesicles . . . . . 100.00 140 98.57 98.57 2.29e-87 . . . . 16546 1 16 no BMRB 18207 . A53T_alpha-synuclein_fibrils . . . . . 100.00 140 97.86 97.86 7.87e-87 . . . . 16546 1 17 no BMRB 18208 . E46K_alpha-synuclein_fibrils . . . . . 100.00 140 97.86 98.57 6.54e-87 . . . . 16546 1 18 no BMRB 18243 . alpha-synuclein_fibrils . . . . . 100.00 140 98.57 98.57 2.29e-87 . . . . 16546 1 19 no BMRB 18857 . alpha_synuclein . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 20 no BMRB 18860 . a-synuclein . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 21 no BMRB 19257 . Alpha-synuclein . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 22 no BMRB 19337 . aSyn . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 23 no BMRB 19338 . aSyn_A53T . . . . . 100.00 140 98.57 98.57 3.39e-88 . . . . 16546 1 24 no BMRB 19344 . aSyn_S87N . . . . . 100.00 140 98.57 99.29 4.66e-88 . . . . 16546 1 25 no BMRB 19345 . aSyn_A53T&S87N . . . . . 100.00 140 97.86 98.57 1.88e-87 . . . . 16546 1 26 no BMRB 19350 . acet_aSyn . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 27 no BMRB 19351 . acet_aSyn_A53T . . . . . 100.00 140 98.57 98.57 3.39e-88 . . . . 16546 1 28 no BMRB 25227 . aSyn-WT . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 29 no BMRB 25228 . H50Q . . . . . 100.00 140 98.57 98.57 1.47e-87 . . . . 16546 1 30 no PDB 1XQ8 . "Human Micelle-Bound Alpha-Synuclein" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 31 no PDB 2KKW . "Slas-Micelle Bound Alpha-Synuclein" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 32 no DBJ BAF82858 . "unnamed protein product [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 33 no DBJ BAG73790 . "synuclein, alpha [synthetic construct]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 34 no EMBL CAG33339 . "SNCA [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 35 no EMBL CAG46454 . "SNCA [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 36 no GB AAA16117 . "AD amyloid [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 37 no GB AAC02114 . "NACP/alpha-synuclein [Homo sapiens]" . . . . . 100.00 140 97.86 97.86 3.67e-87 . . . . 16546 1 38 no GB AAG30302 . "SNCA isoform NACP140 [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 39 no GB AAH13293 . "Synuclein, alpha (non A4 component of amyloid precursor) [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 40 no GB AAI08276 . "Synuclein, alpha (non A4 component of amyloid precursor) [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 41 no REF NP_000336 . "alpha-synuclein isoform NACP140 [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 42 no REF NP_001009158 . "alpha-synuclein [Pan troglodytes]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 43 no REF NP_001032222 . "alpha-synuclein [Sus scrofa]" . . . . . 100.00 140 97.14 97.14 1.60e-85 . . . . 16546 1 44 no REF NP_001129014 . "alpha-synuclein [Pongo abelii]" . . . . . 100.00 140 98.57 98.57 1.06e-87 . . . . 16546 1 45 no REF NP_001139526 . "alpha-synuclein isoform NACP140 [Homo sapiens]" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 46 no SP P37840 . "RecName: Full=Alpha-synuclein; AltName: Full=Non-A beta component of AD amyloid; AltName: Full=Non-A4 component of amyloid prec" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 47 no SP P61139 . "RecName: Full=Alpha-synuclein" . . . . . 100.00 140 98.57 99.29 2.73e-88 . . . . 16546 1 48 no SP P61140 . "RecName: Full=Alpha-synuclein" . . . . . 100.00 140 99.29 99.29 1.04e-88 . . . . 16546 1 49 no SP P61142 . "RecName: Full=Alpha-synuclein" . . . . . 100.00 140 97.86 99.29 3.21e-88 . . . . 16546 1 50 no SP P61143 . "RecName: Full=Alpha-synuclein" . . . . . 100.00 140 97.86 99.29 3.21e-88 . . . . 16546 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 16546 1 2 . ASP . 16546 1 3 . VAL . 16546 1 4 . PHE . 16546 1 5 . MET . 16546 1 6 . LYS . 16546 1 7 . GLY . 16546 1 8 . LEU . 16546 1 9 . SER . 16546 1 10 . LYS . 16546 1 11 . ALA . 16546 1 12 . LYS . 16546 1 13 . GLU . 16546 1 14 . GLY . 16546 1 15 . VAL . 16546 1 16 . VAL . 16546 1 17 . ALA . 16546 1 18 . ALA . 16546 1 19 . ALA . 16546 1 20 . GLU . 16546 1 21 . LYS . 16546 1 22 . THR . 16546 1 23 . LYS . 16546 1 24 . GLN . 16546 1 25 . GLY . 16546 1 26 . VAL . 16546 1 27 . ALA . 16546 1 28 . GLU . 16546 1 29 . ALA . 16546 1 30 . PRO . 16546 1 31 . GLY . 16546 1 32 . LYS . 16546 1 33 . THR . 16546 1 34 . LYS . 16546 1 35 . GLU . 16546 1 36 . GLY . 16546 1 37 . VAL . 16546 1 38 . LEU . 16546 1 39 . TYR . 16546 1 40 . VAL . 16546 1 41 . GLY . 16546 1 42 . SER . 16546 1 43 . LYS . 16546 1 44 . THR . 16546 1 45 . LYS . 16546 1 46 . GLU . 16546 1 47 . GLY . 16546 1 48 . VAL . 16546 1 49 . VAL . 16546 1 50 . HIS . 16546 1 51 . GLY . 16546 1 52 . VAL . 16546 1 53 . ALA . 16546 1 54 . THR . 16546 1 55 . VAL . 16546 1 56 . ALA . 16546 1 57 . GLU . 16546 1 58 . LYS . 16546 1 59 . THR . 16546 1 60 . LYS . 16546 1 61 . GLU . 16546 1 62 . GLN . 16546 1 63 . VAL . 16546 1 64 . THR . 16546 1 65 . ASN . 16546 1 66 . VAL . 16546 1 67 . GLY . 16546 1 68 . GLY . 16546 1 69 . ALA . 16546 1 70 . VAL . 16546 1 71 . VAL . 16546 1 72 . THR . 16546 1 73 . GLY . 16546 1 74 . VAL . 16546 1 75 . THR . 16546 1 76 . ALA . 16546 1 77 . VAL . 16546 1 78 . ALA . 16546 1 79 . GLN . 16546 1 80 . LYS . 16546 1 81 . THR . 16546 1 82 . VAL . 16546 1 83 . GLU . 16546 1 84 . GLY . 16546 1 85 . ALA . 16546 1 86 . GLY . 16546 1 87 . SER . 16546 1 88 . ILE . 16546 1 89 . ALA . 16546 1 90 . ALA . 16546 1 91 . ALA . 16546 1 92 . THR . 16546 1 93 . GLY . 16546 1 94 . PHE . 16546 1 95 . VAL . 16546 1 96 . LYS . 16546 1 97 . LYS . 16546 1 98 . ASP . 16546 1 99 . GLN . 16546 1 100 . LEU . 16546 1 101 . GLY . 16546 1 102 . LYS . 16546 1 103 . ASN . 16546 1 104 . GLU . 16546 1 105 . GLU . 16546 1 106 . GLY . 16546 1 107 . ALA . 16546 1 108 . PRO . 16546 1 109 . GLN . 16546 1 110 . GLU . 16546 1 111 . GLY . 16546 1 112 . ILE . 16546 1 113 . LEU . 16546 1 114 . GLU . 16546 1 115 . ASP . 16546 1 116 . MET . 16546 1 117 . PRO . 16546 1 118 . VAL . 16546 1 119 . ASP . 16546 1 120 . PRO . 16546 1 121 . ASP . 16546 1 122 . ASN . 16546 1 123 . GLU . 16546 1 124 . ALA . 16546 1 125 . TYR . 16546 1 126 . GLU . 16546 1 127 . MET . 16546 1 128 . PRO . 16546 1 129 . SER . 16546 1 130 . GLU . 16546 1 131 . GLU . 16546 1 132 . GLY . 16546 1 133 . TYR . 16546 1 134 . GLN . 16546 1 135 . ASP . 16546 1 136 . TYR . 16546 1 137 . GLU . 16546 1 138 . PRO . 16546 1 139 . GLU . 16546 1 140 . ALA . 16546 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 16546 1 . ASP 2 2 16546 1 . VAL 3 3 16546 1 . PHE 4 4 16546 1 . MET 5 5 16546 1 . LYS 6 6 16546 1 . GLY 7 7 16546 1 . LEU 8 8 16546 1 . SER 9 9 16546 1 . LYS 10 10 16546 1 . ALA 11 11 16546 1 . LYS 12 12 16546 1 . GLU 13 13 16546 1 . GLY 14 14 16546 1 . VAL 15 15 16546 1 . VAL 16 16 16546 1 . ALA 17 17 16546 1 . ALA 18 18 16546 1 . ALA 19 19 16546 1 . GLU 20 20 16546 1 . LYS 21 21 16546 1 . THR 22 22 16546 1 . LYS 23 23 16546 1 . GLN 24 24 16546 1 . GLY 25 25 16546 1 . VAL 26 26 16546 1 . ALA 27 27 16546 1 . GLU 28 28 16546 1 . ALA 29 29 16546 1 . PRO 30 30 16546 1 . GLY 31 31 16546 1 . LYS 32 32 16546 1 . THR 33 33 16546 1 . LYS 34 34 16546 1 . GLU 35 35 16546 1 . GLY 36 36 16546 1 . VAL 37 37 16546 1 . LEU 38 38 16546 1 . TYR 39 39 16546 1 . VAL 40 40 16546 1 . GLY 41 41 16546 1 . SER 42 42 16546 1 . LYS 43 43 16546 1 . THR 44 44 16546 1 . LYS 45 45 16546 1 . GLU 46 46 16546 1 . GLY 47 47 16546 1 . VAL 48 48 16546 1 . VAL 49 49 16546 1 . HIS 50 50 16546 1 . GLY 51 51 16546 1 . VAL 52 52 16546 1 . ALA 53 53 16546 1 . THR 54 54 16546 1 . VAL 55 55 16546 1 . ALA 56 56 16546 1 . GLU 57 57 16546 1 . LYS 58 58 16546 1 . THR 59 59 16546 1 . LYS 60 60 16546 1 . GLU 61 61 16546 1 . GLN 62 62 16546 1 . VAL 63 63 16546 1 . THR 64 64 16546 1 . ASN 65 65 16546 1 . VAL 66 66 16546 1 . GLY 67 67 16546 1 . GLY 68 68 16546 1 . ALA 69 69 16546 1 . VAL 70 70 16546 1 . VAL 71 71 16546 1 . THR 72 72 16546 1 . GLY 73 73 16546 1 . VAL 74 74 16546 1 . THR 75 75 16546 1 . ALA 76 76 16546 1 . VAL 77 77 16546 1 . ALA 78 78 16546 1 . GLN 79 79 16546 1 . LYS 80 80 16546 1 . THR 81 81 16546 1 . VAL 82 82 16546 1 . GLU 83 83 16546 1 . GLY 84 84 16546 1 . ALA 85 85 16546 1 . GLY 86 86 16546 1 . SER 87 87 16546 1 . ILE 88 88 16546 1 . ALA 89 89 16546 1 . ALA 90 90 16546 1 . ALA 91 91 16546 1 . THR 92 92 16546 1 . GLY 93 93 16546 1 . PHE 94 94 16546 1 . VAL 95 95 16546 1 . LYS 96 96 16546 1 . LYS 97 97 16546 1 . ASP 98 98 16546 1 . GLN 99 99 16546 1 . LEU 100 100 16546 1 . GLY 101 101 16546 1 . LYS 102 102 16546 1 . ASN 103 103 16546 1 . GLU 104 104 16546 1 . GLU 105 105 16546 1 . GLY 106 106 16546 1 . ALA 107 107 16546 1 . PRO 108 108 16546 1 . GLN 109 109 16546 1 . GLU 110 110 16546 1 . GLY 111 111 16546 1 . ILE 112 112 16546 1 . LEU 113 113 16546 1 . GLU 114 114 16546 1 . ASP 115 115 16546 1 . MET 116 116 16546 1 . PRO 117 117 16546 1 . VAL 118 118 16546 1 . ASP 119 119 16546 1 . PRO 120 120 16546 1 . ASP 121 121 16546 1 . ASN 122 122 16546 1 . GLU 123 123 16546 1 . ALA 124 124 16546 1 . TYR 125 125 16546 1 . GLU 126 126 16546 1 . MET 127 127 16546 1 . PRO 128 128 16546 1 . SER 129 129 16546 1 . GLU 130 130 16546 1 . GLU 131 131 16546 1 . GLY 132 132 16546 1 . TYR 133 133 16546 1 . GLN 134 134 16546 1 . ASP 135 135 16546 1 . TYR 136 136 16546 1 . GLU 137 137 16546 1 . PRO 138 138 16546 1 . GLU 139 139 16546 1 . ALA 140 140 16546 1 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 16546 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $A30P_alpha-synuclein . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . . . . . . . . . 16546 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 16546 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $A30P_alpha-synuclein . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli BLD21 . . . . . . . . . . . . . . . pET28(a) . . . . . . 16546 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 16546 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'sodium phosphate' 'natural abundance' . . . . . . 20 . . mM . . . . 16546 1 2 protein '[U-15N; U-2H]' . . 1 $A30P_alpha-synuclein . . 0.6 . . mM . . . . 16546 1 3 H2O 'natural abundance' . . . . . . 95 . . % . . . . 16546 1 4 D2O 'natural abundance' . . . . . . 5 . . % . . . . 16546 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 16546 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 20 . mM 16546 1 pH 6.0 . pH 16546 1 pressure 1 . atm 16546 1 temperature 293 . K 16546 1 stop_ save_ ############################ # Computer software used # ############################ save_NMRDraw _Software.Sf_category software _Software.Sf_framecode NMRDraw _Software.Entry_ID 16546 _Software.ID 1 _Software.Name NMRDraw _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 16546 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'peak picking' 16546 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 16546 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 16546 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker DMX . 600 . . . 16546 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 16546 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 16546 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_reference_1 _Chem_shift_reference.Entry_ID 16546 _Chem_shift_reference.ID 1 _Chem_shift_reference.Details 'Not corrected for 2H isotope effect.' loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Indirect_shift_ratio_cit_ID _Chem_shift_ref.Indirect_shift_ratio_cit_label _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Correction_val_cit_ID _Chem_shift_ref.Correction_val_cit_label _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 water protons . . . . ppm 4.82 internal direct 1.0 . . . . . . . . . 16546 1 N 15 water protons . . . . ppm 4.82 internal indirect 0.101329118 . . . . . . . . . 16546 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chem_shift_list_1 _Assigned_chem_shift_list.Entry_ID 16546 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N HSQC' . . . 16546 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 1 3 3 VAL H H 1 8.215 0.01 . 1 . . . . 3 V H . 16546 1 2 . 1 1 3 3 VAL N N 15 120.284 0.05 . 1 . . . . 3 V N . 16546 1 3 . 1 1 4 4 PHE H H 1 8.297 0.01 . 1 . . . . 4 F H . 16546 1 4 . 1 1 4 4 PHE N N 15 123.286 0.05 . 1 . . . . 4 F N . 16546 1 5 . 1 1 5 5 MET H H 1 8.177 0.01 . 1 . . . . 5 M H . 16546 1 6 . 1 1 5 5 MET N N 15 122.162 0.05 . 1 . . . . 5 M N . 16546 1 7 . 1 1 6 6 LYS H H 1 8.221 0.01 . 1 . . . . 6 K H . 16546 1 8 . 1 1 6 6 LYS N N 15 122.474 0.05 . 1 . . . . 6 K N . 16546 1 9 . 1 1 7 7 GLY H H 1 8.364 0.01 . 1 . . . . 7 G H . 16546 1 10 . 1 1 7 7 GLY N N 15 109.685 0.05 . 1 . . . . 7 G N . 16546 1 11 . 1 1 8 8 LEU H H 1 8.021 0.01 . 1 . . . . 8 L H . 16546 1 12 . 1 1 8 8 LEU N N 15 121.521 0.05 . 1 . . . . 8 L N . 16546 1 13 . 1 1 9 9 SER H H 1 8.28 0.01 . 1 . . . . 9 S H . 16546 1 14 . 1 1 9 9 SER N N 15 116.544 0.05 . 1 . . . . 9 S N . 16546 1 15 . 1 1 10 10 LYS H H 1 8.323 0.01 . 1 . . . . 10 K H . 16546 1 16 . 1 1 10 10 LYS N N 15 123.472 0.05 . 1 . . . . 10 K N . 16546 1 17 . 1 1 11 11 ALA H H 1 8.249 0.01 . 1 . . . . 11 A H . 16546 1 18 . 1 1 11 11 ALA N N 15 125.034 0.05 . 1 . . . . 11 A N . 16546 1 19 . 1 1 12 12 LYS H H 1 8.289 0.01 . 1 . . . . 12 K H . 16546 1 20 . 1 1 12 12 LYS N N 15 120.554 0.05 . 1 . . . . 12 K N . 16546 1 21 . 1 1 13 13 GLU H H 1 8.384 0.01 . 1 . . . . 13 E H . 16546 1 22 . 1 1 13 13 GLU N N 15 121.995 0.05 . 1 . . . . 13 E N . 16546 1 23 . 1 1 14 14 GLY H H 1 8.406 0.01 . 1 . . . . 14 G H . 16546 1 24 . 1 1 14 14 GLY N N 15 109.887 0.05 . 1 . . . . 14 G N . 16546 1 25 . 1 1 15 15 VAL H H 1 7.933 0.01 . 1 . . . . 15 V H . 16546 1 26 . 1 1 15 15 VAL N N 15 120.011 0.05 . 1 . . . . 15 V N . 16546 1 27 . 1 1 16 16 VAL H H 1 8.226 0.01 . 1 . . . . 16 V H . 16546 1 28 . 1 1 16 16 VAL N N 15 124.835 0.05 . 1 . . . . 16 V N . 16546 1 29 . 1 1 17 17 ALA H H 1 8.381 0.01 . 1 . . . . 17 A H . 16546 1 30 . 1 1 17 17 ALA N N 15 128.124 0.05 . 1 . . . . 17 A N . 16546 1 31 . 1 1 18 18 ALA H H 1 8.252 0.01 . 1 . . . . 18 A H . 16546 1 32 . 1 1 18 18 ALA N N 15 123.464 0.05 . 1 . . . . 18 A N . 16546 1 33 . 1 1 19 19 ALA H H 1 8.222 0.01 . 1 . . . . 19 A H . 16546 1 34 . 1 1 19 19 ALA N N 15 122.875 0.05 . 1 . . . . 19 A N . 16546 1 35 . 1 1 20 20 GLU H H 1 8.282 0.01 . 1 . . . . 20 E H . 16546 1 36 . 1 1 20 20 GLU N N 15 119.905 0.05 . 1 . . . . 20 E N . 16546 1 37 . 1 1 21 21 LYS H H 1 8.295 0.01 . 1 . . . . 21 K H . 16546 1 38 . 1 1 21 21 LYS N N 15 122.093 0.05 . 1 . . . . 21 K N . 16546 1 39 . 1 1 22 22 THR H H 1 8.078 0.01 . 1 . . . . 22 T H . 16546 1 40 . 1 1 22 22 THR N N 15 114.968 0.05 . 1 . . . . 22 T N . 16546 1 41 . 1 1 23 23 LYS H H 1 8.292 0.01 . 1 . . . . 23 K H . 16546 1 42 . 1 1 23 23 LYS N N 15 123.607 0.05 . 1 . . . . 23 K N . 16546 1 43 . 1 1 24 24 GLN H H 1 8.372 0.01 . 1 . . . . 24 Q H . 16546 1 44 . 1 1 24 24 GLN N N 15 121.551 0.05 . 1 . . . . 24 Q N . 16546 1 45 . 1 1 25 25 GLY H H 1 8.431 0.01 . 1 . . . . 25 G H . 16546 1 46 . 1 1 25 25 GLY N N 15 110.452 0.05 . 1 . . . . 25 G N . 16546 1 47 . 1 1 26 26 VAL H H 1 7.958 0.01 . 1 . . . . 26 V H . 16546 1 48 . 1 1 26 26 VAL N N 15 119.366 0.05 . 1 . . . . 26 V N . 16546 1 49 . 1 1 27 27 ALA H H 1 8.363 0.01 . 1 . . . . 27 A H . 16546 1 50 . 1 1 27 27 ALA N N 15 126.684 0.05 . 1 . . . . 27 A N . 16546 1 51 . 1 1 28 28 GLU H H 1 8.29 0.01 . 1 . . . . 28 E H . 16546 1 52 . 1 1 28 28 GLU N N 15 120.558 0.05 . 1 . . . . 28 E N . 16546 1 53 . 1 1 29 29 ALA H H 1 8.378 0.01 . 1 . . . . 29 A H . 16546 1 54 . 1 1 29 29 ALA N N 15 127.615 0.05 . 1 . . . . 29 A N . 16546 1 55 . 1 1 31 31 GLY H H 1 8.436 0.01 . 1 . . . . 31 G H . 16546 1 56 . 1 1 31 31 GLY N N 15 108.905 0.05 . 1 . . . . 31 G N . 16546 1 57 . 1 1 32 32 LYS H H 1 8.128 0.01 . 1 . . . . 32 K H . 16546 1 58 . 1 1 32 32 LYS N N 15 120.857 0.05 . 1 . . . . 32 K N . 16546 1 59 . 1 1 33 33 THR H H 1 8.201 0.01 . 1 . . . . 33 T H . 16546 1 60 . 1 1 33 33 THR N N 15 115.642 0.05 . 1 . . . . 33 T N . 16546 1 61 . 1 1 34 34 LYS H H 1 8.446 0.01 . 1 . . . . 34 K H . 16546 1 62 . 1 1 34 34 LYS N N 15 123.713 0.05 . 1 . . . . 34 K N . 16546 1 63 . 1 1 35 35 GLU H H 1 8.402 0.01 . 1 . . . . 35 E H . 16546 1 64 . 1 1 35 35 GLU N N 15 121.916 0.05 . 1 . . . . 35 E N . 16546 1 65 . 1 1 36 36 GLY H H 1 8.398 0.01 . 1 . . . . 36 G H . 16546 1 66 . 1 1 36 36 GLY N N 15 109.842 0.05 . 1 . . . . 36 G N . 16546 1 67 . 1 1 37 37 VAL H H 1 7.864 0.01 . 1 . . . . 37 V H . 16546 1 68 . 1 1 37 37 VAL N N 15 119.486 0.05 . 1 . . . . 37 V N . 16546 1 69 . 1 1 38 38 LEU H H 1 8.227 0.01 . 1 . . . . 38 L H . 16546 1 70 . 1 1 38 38 LEU N N 15 125.568 0.05 . 1 . . . . 38 L N . 16546 1 71 . 1 1 39 39 TYR H H 1 8.22 0.01 . 1 . . . . 39 Y H . 16546 1 72 . 1 1 39 39 TYR N N 15 122.189 0.05 . 1 . . . . 39 Y N . 16546 1 73 . 1 1 40 40 VAL H H 1 8.039 0.01 . 1 . . . . 40 V H . 16546 1 74 . 1 1 40 40 VAL N N 15 122.996 0.05 . 1 . . . . 40 V N . 16546 1 75 . 1 1 41 41 GLY H H 1 7.995 0.01 . 1 . . . . 41 G H . 16546 1 76 . 1 1 41 41 GLY N N 15 111.819 0.05 . 1 . . . . 41 G N . 16546 1 77 . 1 1 42 42 SER H H 1 8.207 0.01 . 1 . . . . 42 S H . 16546 1 78 . 1 1 42 42 SER N N 15 115.52 0.05 . 1 . . . . 42 S N . 16546 1 79 . 1 1 43 43 LYS H H 1 8.438 0.01 . 1 . . . . 43 K H . 16546 1 80 . 1 1 43 43 LYS N N 15 123.285 0.05 . 1 . . . . 43 K N . 16546 1 81 . 1 1 44 44 THR H H 1 8.128 0.01 . 1 . . . . 44 T H . 16546 1 82 . 1 1 44 44 THR N N 15 115.28 0.05 . 1 . . . . 44 T N . 16546 1 83 . 1 1 45 45 LYS H H 1 8.372 0.01 . 1 . . . . 45 K H . 16546 1 84 . 1 1 45 45 LYS N N 15 123.544 0.05 . 1 . . . . 45 K N . 16546 1 85 . 1 1 46 46 GLU H H 1 8.415 0.01 . 1 . . . . 46 E H . 16546 1 86 . 1 1 46 46 GLU N N 15 121.895 0.05 . 1 . . . . 46 E N . 16546 1 87 . 1 1 47 47 GLY H H 1 8.377 0.01 . 1 . . . . 47 G H . 16546 1 88 . 1 1 47 47 GLY N N 15 109.835 0.05 . 1 . . . . 47 G N . 16546 1 89 . 1 1 48 48 VAL H H 1 7.875 0.01 . 1 . . . . 48 V H . 16546 1 90 . 1 1 48 48 VAL N N 15 119.739 0.05 . 1 . . . . 48 V N . 16546 1 91 . 1 1 49 49 VAL H H 1 8.227 0.01 . 1 . . . . 49 V H . 16546 1 92 . 1 1 49 49 VAL N N 15 124.53 0.05 . 1 . . . . 49 V N . 16546 1 93 . 1 1 50 50 HIS H H 1 8.59 0.01 . 1 . . . . 50 H H . 16546 1 94 . 1 1 50 50 HIS N N 15 123.289 0.05 . 1 . . . . 50 H N . 16546 1 95 . 1 1 51 51 GLY H H 1 8.429 0.01 . 1 . . . . 51 G H . 16546 1 96 . 1 1 51 51 GLY N N 15 110.468 0.05 . 1 . . . . 51 G N . 16546 1 97 . 1 1 52 52 VAL H H 1 8.034 0.01 . 1 . . . . 52 V H . 16546 1 98 . 1 1 52 52 VAL N N 15 119.483 0.05 . 1 . . . . 52 V N . 16546 1 99 . 1 1 53 53 ALA H H 1 8.437 0.01 . 1 . . . . 53 A H . 16546 1 100 . 1 1 53 53 ALA N N 15 127.828 0.05 . 1 . . . . 53 A N . 16546 1 101 . 1 1 54 54 THR H H 1 8.171 0.01 . 1 . . . . 54 T H . 16546 1 102 . 1 1 54 54 THR N N 15 114.615 0.05 . 1 . . . . 54 T N . 16546 1 103 . 1 1 55 55 VAL H H 1 8.179 0.01 . 1 . . . . 55 V H . 16546 1 104 . 1 1 55 55 VAL N N 15 122.695 0.05 . 1 . . . . 55 V N . 16546 1 105 . 1 1 56 56 ALA H H 1 8.359 0.01 . 1 . . . . 56 A H . 16546 1 106 . 1 1 56 56 ALA N N 15 127.663 0.05 . 1 . . . . 56 A N . 16546 1 107 . 1 1 57 57 GLU H H 1 8.313 0.01 . 1 . . . . 57 E H . 16546 1 108 . 1 1 57 57 GLU N N 15 120.658 0.05 . 1 . . . . 57 E N . 16546 1 109 . 1 1 58 58 LYS H H 1 8.375 0.01 . 1 . . . . 58 K H . 16546 1 110 . 1 1 58 58 LYS N N 15 122.548 0.05 . 1 . . . . 58 K N . 16546 1 111 . 1 1 59 59 THR H H 1 8.154 0.01 . 1 . . . . 59 T H . 16546 1 112 . 1 1 59 59 THR N N 15 115.636 0.05 . 1 . . . . 59 T N . 16546 1 113 . 1 1 60 60 LYS H H 1 8.327 0.01 . 1 . . . . 60 K H . 16546 1 114 . 1 1 60 60 LYS N N 15 123.402 0.05 . 1 . . . . 60 K N . 16546 1 115 . 1 1 61 61 GLU H H 1 8.377 0.01 . 1 . . . . 61 E H . 16546 1 116 . 1 1 61 61 GLU N N 15 121.838 0.05 . 1 . . . . 61 E N . 16546 1 117 . 1 1 62 62 GLN H H 1 8.371 0.01 . 1 . . . . 62 Q H . 16546 1 118 . 1 1 62 62 GLN N N 15 121.556 0.05 . 1 . . . . 62 Q N . 16546 1 119 . 1 1 63 63 VAL H H 1 8.226 0.01 . 1 . . . . 63 V H . 16546 1 120 . 1 1 63 63 VAL N N 15 121.678 0.05 . 1 . . . . 63 V N . 16546 1 121 . 1 1 64 64 THR H H 1 8.247 0.01 . 1 . . . . 64 T H . 16546 1 122 . 1 1 64 64 THR N N 15 117.788 0.05 . 1 . . . . 64 T N . 16546 1 123 . 1 1 65 65 ASN H H 1 8.465 0.01 . 1 . . . . 65 N H . 16546 1 124 . 1 1 65 65 ASN N N 15 121.581 0.05 . 1 . . . . 65 N N . 16546 1 125 . 1 1 66 66 VAL H H 1 8.19 0.01 . 1 . . . . 66 V H . 16546 1 126 . 1 1 66 66 VAL N N 15 120.577 0.05 . 1 . . . . 66 V N . 16546 1 127 . 1 1 67 67 GLY H H 1 8.491 0.01 . 1 . . . . 67 G H . 16546 1 128 . 1 1 67 67 GLY N N 15 112.342 0.05 . 1 . . . . 67 G N . 16546 1 129 . 1 1 68 68 GLY H H 1 8.185 0.01 . 1 . . . . 68 G H . 16546 1 130 . 1 1 68 68 GLY N N 15 108.764 0.05 . 1 . . . . 68 G N . 16546 1 131 . 1 1 69 69 ALA H H 1 8.115 0.01 . 1 . . . . 69 A H . 16546 1 132 . 1 1 69 69 ALA N N 15 123.661 0.05 . 1 . . . . 69 A N . 16546 1 133 . 1 1 70 70 VAL H H 1 8.151 0.01 . 1 . . . . 70 V H . 16546 1 134 . 1 1 70 70 VAL N N 15 120.248 0.05 . 1 . . . . 70 V N . 16546 1 135 . 1 1 71 71 VAL H H 1 8.319 0.01 . 1 . . . . 71 V H . 16546 1 136 . 1 1 71 71 VAL N N 15 124.987 0.05 . 1 . . . . 71 V N . 16546 1 137 . 1 1 72 72 THR H H 1 8.247 0.01 . 1 . . . . 72 T H . 16546 1 138 . 1 1 72 72 THR N N 15 118.283 0.05 . 1 . . . . 72 T N . 16546 1 139 . 1 1 73 73 GLY H H 1 8.376 0.01 . 1 . . . . 73 G H . 16546 1 140 . 1 1 73 73 GLY N N 15 111.121 0.05 . 1 . . . . 73 G N . 16546 1 141 . 1 1 74 74 VAL H H 1 8.032 0.01 . 1 . . . . 74 V H . 16546 1 142 . 1 1 74 74 VAL N N 15 119.454 0.05 . 1 . . . . 74 V N . 16546 1 143 . 1 1 75 75 THR H H 1 8.237 0.01 . 1 . . . . 75 T H . 16546 1 144 . 1 1 75 75 THR N N 15 118.531 0.05 . 1 . . . . 75 T N . 16546 1 145 . 1 1 76 76 ALA H H 1 8.305 0.01 . 1 . . . . 76 A H . 16546 1 146 . 1 1 76 76 ALA N N 15 127.036 0.05 . 1 . . . . 76 A N . 16546 1 147 . 1 1 77 77 VAL H H 1 8.076 0.01 . 1 . . . . 77 V H . 16546 1 148 . 1 1 77 77 VAL N N 15 119.78 0.05 . 1 . . . . 77 V N . 16546 1 149 . 1 1 78 78 ALA H H 1 8.339 0.01 . 1 . . . . 78 A H . 16546 1 150 . 1 1 78 78 ALA N N 15 127.725 0.05 . 1 . . . . 78 A N . 16546 1 151 . 1 1 79 79 GLN H H 1 8.326 0.01 . 1 . . . . 79 Q H . 16546 1 152 . 1 1 79 79 GLN N N 15 120.086 0.05 . 1 . . . . 79 Q N . 16546 1 153 . 1 1 80 80 LYS H H 1 8.376 0.01 . 1 . . . . 80 K H . 16546 1 154 . 1 1 80 80 LYS N N 15 123.019 0.05 . 1 . . . . 80 K N . 16546 1 155 . 1 1 81 81 THR H H 1 8.235 0.01 . 1 . . . . 81 T H . 16546 1 156 . 1 1 81 81 THR N N 15 116.66 0.05 . 1 . . . . 81 T N . 16546 1 157 . 1 1 82 82 VAL H H 1 8.246 0.01 . 1 . . . . 82 V H . 16546 1 158 . 1 1 82 82 VAL N N 15 122.703 0.05 . 1 . . . . 82 V N . 16546 1 159 . 1 1 83 83 GLU H H 1 8.512 0.01 . 1 . . . . 83 E H . 16546 1 160 . 1 1 83 83 GLU N N 15 124.964 0.05 . 1 . . . . 83 E N . 16546 1 161 . 1 1 84 84 GLY H H 1 8.455 0.01 . 1 . . . . 84 G H . 16546 1 162 . 1 1 84 84 GLY N N 15 110.489 0.05 . 1 . . . . 84 G N . 16546 1 163 . 1 1 85 85 ALA H H 1 8.213 0.01 . 1 . . . . 85 A H . 16546 1 164 . 1 1 85 85 ALA N N 15 123.842 0.05 . 1 . . . . 85 A N . 16546 1 165 . 1 1 86 86 GLY H H 1 8.434 0.01 . 1 . . . . 86 G H . 16546 1 166 . 1 1 86 86 GLY N N 15 108.013 0.05 . 1 . . . . 86 G N . 16546 1 167 . 1 1 87 87 SER H H 1 8.111 0.01 . 1 . . . . 87 S H . 16546 1 168 . 1 1 87 87 SER N N 15 115.602 0.05 . 1 . . . . 87 S N . 16546 1 169 . 1 1 88 88 ILE H H 1 8.142 0.01 . 1 . . . . 88 I H . 16546 1 170 . 1 1 88 88 ILE N N 15 122.613 0.05 . 1 . . . . 88 I N . 16546 1 171 . 1 1 89 89 ALA H H 1 8.29 0.01 . 1 . . . . 89 A H . 16546 1 172 . 1 1 89 89 ALA N N 15 127.696 0.05 . 1 . . . . 89 A N . 16546 1 173 . 1 1 90 90 ALA H H 1 8.148 0.01 . 1 . . . . 90 A H . 16546 1 174 . 1 1 90 90 ALA N N 15 123.088 0.05 . 1 . . . . 90 A N . 16546 1 175 . 1 1 91 91 ALA H H 1 8.232 0.01 . 1 . . . . 91 A H . 16546 1 176 . 1 1 91 91 ALA N N 15 123.147 0.05 . 1 . . . . 91 A N . 16546 1 177 . 1 1 92 92 THR H H 1 8.038 0.01 . 1 . . . . 92 T H . 16546 1 178 . 1 1 92 92 THR N N 15 112.442 0.05 . 1 . . . . 92 T N . 16546 1 179 . 1 1 93 93 GLY H H 1 8.249 0.01 . 1 . . . . 93 G H . 16546 1 180 . 1 1 93 93 GLY N N 15 110.483 0.05 . 1 . . . . 93 G N . 16546 1 181 . 1 1 94 94 PHE H H 1 8.046 0.01 . 1 . . . . 94 F H . 16546 1 182 . 1 1 94 94 PHE N N 15 120.23 0.05 . 1 . . . . 94 F N . 16546 1 183 . 1 1 95 95 VAL H H 1 8.009 0.01 . 1 . . . . 95 V H . 16546 1 184 . 1 1 95 95 VAL N N 15 123.389 0.05 . 1 . . . . 95 V N . 16546 1 185 . 1 1 96 96 LYS H H 1 8.338 0.01 . 1 . . . . 96 K H . 16546 1 186 . 1 1 96 96 LYS N N 15 126.104 0.05 . 1 . . . . 96 K N . 16546 1 187 . 1 1 97 97 LYS H H 1 8.419 0.01 . 1 . . . . 97 K H . 16546 1 188 . 1 1 97 97 LYS N N 15 123.576 0.05 . 1 . . . . 97 K N . 16546 1 189 . 1 1 98 98 ASP H H 1 8.362 0.01 . 1 . . . . 98 D H . 16546 1 190 . 1 1 98 98 ASP N N 15 121 0.05 . 1 . . . . 98 D N . 16546 1 191 . 1 1 99 99 GLN H H 1 8.299 0.01 . 1 . . . . 99 Q H . 16546 1 192 . 1 1 99 99 GLN N N 15 120.034 0.05 . 1 . . . . 99 Q N . 16546 1 193 . 1 1 100 100 LEU H H 1 8.244 0.01 . 1 . . . . 100 L H . 16546 1 194 . 1 1 100 100 LEU N N 15 122.7 0.05 . 1 . . . . 100 L N . 16546 1 195 . 1 1 101 101 GLY H H 1 8.417 0.01 . 1 . . . . 101 G H . 16546 1 196 . 1 1 101 101 GLY N N 15 109.596 0.05 . 1 . . . . 101 G N . 16546 1 197 . 1 1 102 102 LYS H H 1 8.161 0.01 . 1 . . . . 102 K H . 16546 1 198 . 1 1 102 102 LYS N N 15 120.7 0.05 . 1 . . . . 102 K N . 16546 1 199 . 1 1 103 103 ASN H H 1 8.567 0.01 . 1 . . . . 103 N H . 16546 1 200 . 1 1 103 103 ASN N N 15 119.837 0.05 . 1 . . . . 103 N N . 16546 1 201 . 1 1 104 104 GLU H H 1 8.434 0.01 . 1 . . . . 104 E H . 16546 1 202 . 1 1 104 104 GLU N N 15 121.228 0.05 . 1 . . . . 104 E N . 16546 1 203 . 1 1 105 105 GLU H H 1 8.418 0.01 . 1 . . . . 105 E H . 16546 1 204 . 1 1 105 105 GLU N N 15 121.631 0.05 . 1 . . . . 105 E N . 16546 1 205 . 1 1 106 106 GLY H H 1 8.376 0.01 . 1 . . . . 106 G H . 16546 1 206 . 1 1 106 106 GLY N N 15 110.051 0.05 . 1 . . . . 106 G N . 16546 1 207 . 1 1 107 107 ALA H H 1 8.067 0.01 . 1 . . . . 107 A H . 16546 1 208 . 1 1 107 107 ALA N N 15 124.804 0.05 . 1 . . . . 107 A N . 16546 1 209 . 1 1 109 109 GLN H H 1 8.523 0.01 . 1 . . . . 109 Q H . 16546 1 210 . 1 1 109 109 GLN N N 15 120.938 0.05 . 1 . . . . 109 Q N . 16546 1 211 . 1 1 110 110 GLU H H 1 8.466 0.01 . 1 . . . . 110 E H . 16546 1 212 . 1 1 110 110 GLU N N 15 122.285 0.05 . 1 . . . . 110 E N . 16546 1 213 . 1 1 111 111 GLY H H 1 8.426 0.01 . 1 . . . . 111 G H . 16546 1 214 . 1 1 111 111 GLY N N 15 110.066 0.05 . 1 . . . . 111 G N . 16546 1 215 . 1 1 112 112 ILE H H 1 7.943 0.01 . 1 . . . . 112 I H . 16546 1 216 . 1 1 112 112 ILE N N 15 120.116 0.05 . 1 . . . . 112 I N . 16546 1 217 . 1 1 113 113 LEU H H 1 8.346 0.01 . 1 . . . . 113 L H . 16546 1 218 . 1 1 113 113 LEU N N 15 126.744 0.05 . 1 . . . . 113 L N . 16546 1 219 . 1 1 114 114 GLU H H 1 8.368 0.01 . 1 . . . . 114 E H . 16546 1 220 . 1 1 114 114 GLU N N 15 122.032 0.05 . 1 . . . . 114 E N . 16546 1 221 . 1 1 115 115 ASP H H 1 8.312 0.01 . 1 . . . . 115 D H . 16546 1 222 . 1 1 115 115 ASP N N 15 121.202 0.05 . 1 . . . . 115 D N . 16546 1 223 . 1 1 116 116 MET H H 1 8.209 0.01 . 1 . . . . 116 M H . 16546 1 224 . 1 1 116 116 MET N N 15 121.851 0.05 . 1 . . . . 116 M N . 16546 1 225 . 1 1 118 118 VAL H H 1 8.227 0.01 . 1 . . . . 118 V H . 16546 1 226 . 1 1 118 118 VAL N N 15 120.574 0.05 . 1 . . . . 118 V N . 16546 1 227 . 1 1 119 119 ASP H H 1 8.454 0.01 . 1 . . . . 119 D H . 16546 1 228 . 1 1 119 119 ASP N N 15 125.597 0.05 . 1 . . . . 119 D N . 16546 1 229 . 1 1 121 121 ASP H H 1 8.341 0.01 . 1 . . . . 121 D H . 16546 1 230 . 1 1 121 121 ASP N N 15 119.15 0.05 . 1 . . . . 121 D N . 16546 1 231 . 1 1 122 122 ASN H H 1 8.1 0.01 . 1 . . . . 122 N H . 16546 1 232 . 1 1 122 122 ASN N N 15 118.967 0.05 . 1 . . . . 122 N N . 16546 1 233 . 1 1 123 123 GLU H H 1 8.315 0.01 . 1 . . . . 123 E H . 16546 1 234 . 1 1 123 123 GLU N N 15 121.43 0.05 . 1 . . . . 123 E N . 16546 1 235 . 1 1 124 124 ALA H H 1 8.169 0.01 . 1 . . . . 124 A H . 16546 1 236 . 1 1 124 124 ALA N N 15 124.267 0.05 . 1 . . . . 124 A N . 16546 1 237 . 1 1 125 125 TYR H H 1 7.984 0.01 . 1 . . . . 125 Y H . 16546 1 238 . 1 1 125 125 TYR N N 15 119.723 0.05 . 1 . . . . 125 Y N . 16546 1 239 . 1 1 126 126 GLU H H 1 8.101 0.01 . 1 . . . . 126 E H . 16546 1 240 . 1 1 126 126 GLU N N 15 123.379 0.05 . 1 . . . . 126 E N . 16546 1 241 . 1 1 127 127 MET H H 1 8.368 0.01 . 1 . . . . 127 M H . 16546 1 242 . 1 1 127 127 MET N N 15 123.63 0.05 . 1 . . . . 127 M N . 16546 1 243 . 1 1 129 129 SER H H 1 8.422 0.01 . 1 . . . . 129 S H . 16546 1 244 . 1 1 129 129 SER N N 15 116.478 0.05 . 1 . . . . 129 S N . 16546 1 245 . 1 1 130 130 GLU H H 1 8.516 0.01 . 1 . . . . 130 E H . 16546 1 246 . 1 1 130 130 GLU N N 15 123.001 0.05 . 1 . . . . 130 E N . 16546 1 247 . 1 1 131 131 GLU H H 1 8.418 0.01 . 1 . . . . 131 E H . 16546 1 248 . 1 1 131 131 GLU N N 15 121.811 0.05 . 1 . . . . 131 E N . 16546 1 249 . 1 1 132 132 GLY H H 1 8.35 0.01 . 1 . . . . 132 G H . 16546 1 250 . 1 1 132 132 GLY N N 15 109.74 0.05 . 1 . . . . 132 G N . 16546 1 251 . 1 1 133 133 TYR H H 1 8.024 0.01 . 1 . . . . 133 Y H . 16546 1 252 . 1 1 133 133 TYR N N 15 120.224 0.05 . 1 . . . . 133 Y N . 16546 1 253 . 1 1 134 134 GLN H H 1 8.181 0.01 . 1 . . . . 134 Q H . 16546 1 254 . 1 1 134 134 GLN N N 15 122.519 0.05 . 1 . . . . 134 Q N . 16546 1 255 . 1 1 135 135 ASP H H 1 8.2 0.01 . 1 . . . . 135 D H . 16546 1 256 . 1 1 135 135 ASP N N 15 121.508 0.05 . 1 . . . . 135 D N . 16546 1 257 . 1 1 136 136 TYR H H 1 8.015 0.01 . 1 . . . . 136 Y H . 16546 1 258 . 1 1 136 136 TYR N N 15 120.486 0.05 . 1 . . . . 136 Y N . 16546 1 259 . 1 1 137 137 GLU H H 1 8.201 0.01 . 1 . . . . 137 E H . 16546 1 260 . 1 1 137 137 GLU N N 15 125.099 0.05 . 1 . . . . 137 E N . 16546 1 261 . 1 1 139 139 GLU H H 1 8.455 0.01 . 1 . . . . 139 E H . 16546 1 262 . 1 1 139 139 GLU N N 15 121.333 0.05 . 1 . . . . 139 E N . 16546 1 263 . 1 1 140 140 ALA H H 1 7.942 0.01 . 1 . . . . 140 A H . 16546 1 264 . 1 1 140 140 ALA N N 15 130.566 0.05 . 1 . . . . 140 A N . 16546 1 stop_ save_