data_10140

#######################
#  Entry information  #
#######################

save_entry_information
   _Saveframe_category      entry_information

   _Entry_title            
;
Backbone NMR assignments of  of RimM complexed with rS19
;
   _BMRB_accession_number   10140
   _BMRB_flat_file_name     bmr10140.str
   _Entry_type              original
   _Submission_date         2007-06-13
   _Accession_date          2007-06-15
   _Entry_origination       author
   _NMR_STAR_version        2.1.1
   _Experimental_method     NMR
   _Details                 .

   loop_
      _Author_ordinal
      _Author_family_name
      _Author_given_name
      _Author_middle_initials
      _Author_family_title

      1 Suzuki     S. . . 
      2 Tatsuguchi A. . . 
      3 Matsumoto  E. . . 
      4 Kawazoe    M. . . 
      5 Kaminishi  T. . . 
      6 Shirouzu   M. . . 
      7 Muto       Y. . . 
      8 Takemoto   C. . . 
      9 Yokoyama   S. . . 

   stop_

   loop_
      _Saveframe_category_type
      _Saveframe_category_type_count

      assigned_chemical_shifts 1 

   stop_

   loop_
      _Data_type
      _Data_type_count

      "1H chemical shifts"  141 
      "13C chemical shifts" 156 
      "15N chemical shifts" 141 

   stop_

   loop_
      _Revision_date
      _Revision_keyword
      _Revision_author
      _Revision_detail

      2008-06-12 original author . 

   stop_

   loop_
      _Related_BMRB_accession_number
      _Relationship

      10138 'N-terminal construct of RimM (residues 1-85)'    
      10139 'full-length RimM (residues 1-162) in free state' 

   stop_

   _Original_release_date   2008-06-12

save_


#############################
#  Citation for this entry  #
#############################

save_citation_1
   _Saveframe_category           entry_citation

   _Citation_full                .
   _Citation_title              'Structural characterization of the ribosome maturation protein, RimM'
   _Citation_status              published
   _Citation_type                journal
   _CAS_abstract_code            .
   _MEDLINE_UI_code              .
   _PubMed_ID                    17616598

   loop_
      _Author_ordinal
      _Author_family_name
      _Author_given_name
      _Author_middle_initials
      _Author_family_title

      1 Suzuki     S. . . 
      2 Tatsuguchi A. . . 
      3 Matsumoto  E. . . 
      4 Kawazoe    M. . . 
      5 Kaminishi  T. . . 
      6 Shirouzu   M. . . 
      7 Muto       Y. . . 
      8 Takemoto   C. . . 
      9 Yokoyama   S. . . 

   stop_

   _Journal_abbreviation        'J. Bacteriol.'
   _Journal_volume               189
   _Journal_issue                17
   _Journal_CSD                  .
   _Book_chapter_title           .
   _Book_volume                  .
   _Book_series                  .
   _Book_ISBN                    .
   _Conference_state_province    .
   _Conference_abstract_number   .
   _Page_first                   .
   _Page_last                    6397
   _Year                         6406
   _Details                      2007

save_


##################################
#  Molecular system description  #
##################################

save_assembly
   _Saveframe_category         molecular_system

   _Mol_system_name           'RimM complexed to S19'
   _Enzyme_commission_number   .

   loop_
      _Mol_system_component_name
      _Mol_label

      RimM $entity_1 
      rS19 $entity_2 

   stop_

   _System_molecular_weight    .
   _System_physical_state      native
   _System_oligomer_state     'protein-protein complex'
   _System_paramagnetic        no
   _System_thiol_state        'not present'
   _Database_query_date        .
   _Details                   'RimM complexed to ribosomal protein S19 from T. thermophilus'

save_


    ########################
    #  Monomeric polymers  #
    ########################

save_entity_1
   _Saveframe_category                          monomeric_polymer

   _Mol_type                                    polymer
   _Mol_polymer_class                           protein
   _Name_common                                 RimM
   _Molecular_mass                              .
   _Mol_thiol_state                            'not present'
   _Details                                     .

   	##############################
   	#  Polymer residue sequence  #
   	##############################
   
      _Residue_count                               162
   _Mol_residue_sequence                       
;
MRLVEIGRFGAPYALKGGLR
FRGEPVVLHLERVYVEGHGW
RAIEDLYRVGEELVVHLAGV
TDRTLAEALVGLRVYAEVAD
LPPLEEGRYYYFALIGLPVY
VEGRQVGEVVDILDAGAQDV
LIIRGVGERLRDRAERLVPL
QAPYVRVEEGSIHVDPIPGL
FD
;

   loop_
      _Residue_seq_code
      _Residue_label

        1 MET    2 ARG    3 LEU    4 VAL    5 GLU 
        6 ILE    7 GLY    8 ARG    9 PHE   10 GLY 
       11 ALA   12 PRO   13 TYR   14 ALA   15 LEU 
       16 LYS   17 GLY   18 GLY   19 LEU   20 ARG 
       21 PHE   22 ARG   23 GLY   24 GLU   25 PRO 
       26 VAL   27 VAL   28 LEU   29 HIS   30 LEU 
       31 GLU   32 ARG   33 VAL   34 TYR   35 VAL 
       36 GLU   37 GLY   38 HIS   39 GLY   40 TRP 
       41 ARG   42 ALA   43 ILE   44 GLU   45 ASP 
       46 LEU   47 TYR   48 ARG   49 VAL   50 GLY 
       51 GLU   52 GLU   53 LEU   54 VAL   55 VAL 
       56 HIS   57 LEU   58 ALA   59 GLY   60 VAL 
       61 THR   62 ASP   63 ARG   64 THR   65 LEU 
       66 ALA   67 GLU   68 ALA   69 LEU   70 VAL 
       71 GLY   72 LEU   73 ARG   74 VAL   75 TYR 
       76 ALA   77 GLU   78 VAL   79 ALA   80 ASP 
       81 LEU   82 PRO   83 PRO   84 LEU   85 GLU 
       86 GLU   87 GLY   88 ARG   89 TYR   90 TYR 
       91 TYR   92 PHE   93 ALA   94 LEU   95 ILE 
       96 GLY   97 LEU   98 PRO   99 VAL  100 TYR 
      101 VAL  102 GLU  103 GLY  104 ARG  105 GLN 
      106 VAL  107 GLY  108 GLU  109 VAL  110 VAL 
      111 ASP  112 ILE  113 LEU  114 ASP  115 ALA 
      116 GLY  117 ALA  118 GLN  119 ASP  120 VAL 
      121 LEU  122 ILE  123 ILE  124 ARG  125 GLY 
      126 VAL  127 GLY  128 GLU  129 ARG  130 LEU 
      131 ARG  132 ASP  133 ARG  134 ALA  135 GLU 
      136 ARG  137 LEU  138 VAL  139 PRO  140 LEU 
      141 GLN  142 ALA  143 PRO  144 TYR  145 VAL 
      146 ARG  147 VAL  148 GLU  149 GLU  150 GLY 
      151 SER  152 ILE  153 HIS  154 VAL  155 ASP 
      156 PRO  157 ILE  158 PRO  159 GLY  160 LEU 
      161 PHE  162 ASP 

   stop_

   _Sequence_homology_query_date                .
   _Sequence_homology_query_revised_last_date   2015-08-05

   loop_
      _Database_name
      _Database_accession_code
      _Database_entry_mol_name
      _Sequence_query_to_submitted_percentage
      _Sequence_subject_length
      _Sequence_identity
      _Sequence_positive
      _Sequence_homology_expectation_value

      BMRB        10138 "RIEF fold"                                                                                     52.47  85 100.00 100.00 2.78e-48  
      BMRB        10139  RimM                                                                                          100.00 162 100.00 100.00 1.44e-103 
      PDB  2DOG          "Solution Structure Of The N-Terminal Domain Of Rimm From Thermus Thermophilus Hb8"             52.47  85 100.00 100.00 2.78e-48  
      PDB  2DYI          "Crystal Structure Of 16s Ribosomal Rna Processing Protein Rimm From Thermus Thermophilus Hb8" 100.00 162 100.00 100.00 1.44e-103 
      PDB  3A1P          "Structure Of Ribosome Maturation Protein Rimm And Ribosomal Protein S19"                      100.00 162 100.00 100.00 1.44e-103 
      DBJ  BAD70856      "16S rRNA processing protein RimM [Thermus thermophilus HB8]"                                  100.00 162 100.00 100.00 1.44e-103 
      GB   AAS81019      "16S rRNA processing protein rimM [Thermus thermophilus HB27]"                                 100.00 162  99.38  99.38 1.30e-102 
      GB   AEG33454      "16S rRNA processing protein RimM [Thermus thermophilus SG0.5JP17-16]"                         100.00 162  99.38  99.38 1.30e-102 
      GB   AFH38925      "16S rRNA processing protein RimM [Thermus thermophilus JL-18]"                                100.00 162  99.38  99.38 1.30e-102 
      GB   EIA39096      "16S rRNA-processing protein RimM [Thermus sp. RL]"                                            100.00 162  99.38  99.38 1.30e-102 
      REF  WP_008632437  "MULTISPECIES: ribosome maturation factor RimM [Thermus]"                                      100.00 162  99.38  99.38 1.30e-102 
      REF  WP_011228392  "ribosome maturation factor RimM [Thermus thermophilus]"                                       100.00 162 100.00 100.00 1.44e-103 
      REF  YP_144299     "16S rRNA-processing protein RimM [Thermus thermophilus HB8]"                                  100.00 162 100.00 100.00 1.44e-103 
      SP   Q5SJH5        "RecName: Full=Ribosome maturation factor RimM"                                                100.00 162 100.00 100.00 1.44e-103 
      SP   Q72JU7        "RecName: Full=Ribosome maturation factor RimM"                                                100.00 162  99.38  99.38 1.30e-102 

   stop_

save_


save_entity_2
   _Saveframe_category                          monomeric_polymer

   _Mol_type                                    polymer
   _Mol_polymer_class                           protein
   _Name_common                                 rS19
   _Molecular_mass                              .
   _Mol_thiol_state                            'not present'
   _Details                                     .
   _Residue_count                               93
   _Mol_residue_sequence                       
;
MPRSLKKGVFVDDHLLEKVL
ELNAKGEKRLIKTWSRRSTI
VPEMVGHTIAVYNGKQHVPV
YITENMVGHKLGEFAPTRTY
RGHGKEAKATKKK
;

   loop_
      _Residue_seq_code
      _Residue_label

       1 MET   2 PRO   3 ARG   4 SER   5 LEU 
       6 LYS   7 LYS   8 GLY   9 VAL  10 PHE 
      11 VAL  12 ASP  13 ASP  14 HIS  15 LEU 
      16 LEU  17 GLU  18 LYS  19 VAL  20 LEU 
      21 GLU  22 LEU  23 ASN  24 ALA  25 LYS 
      26 GLY  27 GLU  28 LYS  29 ARG  30 LEU 
      31 ILE  32 LYS  33 THR  34 TRP  35 SER 
      36 ARG  37 ARG  38 SER  39 THR  40 ILE 
      41 VAL  42 PRO  43 GLU  44 MET  45 VAL 
      46 GLY  47 HIS  48 THR  49 ILE  50 ALA 
      51 VAL  52 TYR  53 ASN  54 GLY  55 LYS 
      56 GLN  57 HIS  58 VAL  59 PRO  60 VAL 
      61 TYR  62 ILE  63 THR  64 GLU  65 ASN 
      66 MET  67 VAL  68 GLY  69 HIS  70 LYS 
      71 LEU  72 GLY  73 GLU  74 PHE  75 ALA 
      76 PRO  77 THR  78 ARG  79 THR  80 TYR 
      81 ARG  82 GLY  83 HIS  84 GLY  85 LYS 
      86 GLU  87 ALA  88 LYS  89 ALA  90 THR 
      91 LYS  92 LYS  93 LYS 

   stop_

   _Sequence_homology_query_date                .
   _Sequence_homology_query_revised_last_date   .

save_


    ####################
    #  Natural source  #
    ####################

save_natural_source
   _Saveframe_category   natural_source


   loop_
      _Mol_label
      _Organism_name_common
      _NCBI_taxonomy_ID
      _Superkingdom
      _Kingdom
      _Genus
      _Species

      $entity_1 'Thermus thermophilus' 274 Bacteria . Thermus thermophilus 
      $entity_2 'Thermus thermophilus' 274 Bacteria . Thermus thermophilus 

   stop_

save_


    #########################
    #  Experimental source  #
    #########################

save_experimental_source
   _Saveframe_category   experimental_source


   loop_
      _Mol_label
      _Production_method
      _Host_organism_name_common
      _Genus
      _Species
      _Strain
      _Vector_type
      _Vector_name

      $entity_1 'recombinant technology' 'E. coli' Escherichia coli . plasmid PC011033-04 

   stop_

save_


#####################################
#  Sample contents and methodology  #
#####################################
	 
    ########################
    #  Sample description  #
    ########################

save_sample_1
   _Saveframe_category   sample

   _Sample_type          solution
   _Details              .

   loop_
      _Mol_label
      _Concentration_value
      _Concentration_value_units
      _Isotopic_labeling

      $entity_1   0.03 mM '[U-13C; U-15N]' 
      $entity_2   0.03 mM  .               
       HEPES     20    mM  .               
       D2O       10    %   .               
       H2O       90    %   .               
       NaCl     300    mM  .               

   stop_

save_


############################
#  Computer software used  #
############################

save_software_1
   _Saveframe_category   software

   _Name                 xwinnmr
   _Version              3.5

   loop_
      _Vendor
      _Address
      _Electronic_address

      Bruker . . 

   stop_

   loop_
      _Task

      collection 

   stop_

   _Details              .

save_


save_software_2
   _Saveframe_category   software

   _Name                 NMRPipe
   _Version              20031121

   loop_
      _Vendor
      _Address
      _Electronic_address

      'Delaglio, F.' . . 

   stop_

   loop_
      _Task

      processing 

   stop_

   _Details              .

save_


save_software_3
   _Saveframe_category   software

   _Name                 NMRView
   _Version              5.0.4

   loop_
      _Vendor
      _Address
      _Electronic_address

      'Johnson, B.A.' . . 

   stop_

   loop_
      _Task

      'data analysis' 

   stop_

   _Details              .

save_


save_software_4
   _Saveframe_category   software

   _Name                 Kujira
   _Version              0.9321

   loop_
      _Vendor
      _Address
      _Electronic_address

      'Kobayashi, N.' . . 

   stop_

   loop_
      _Task

      'data analysis' 

   stop_

   _Details              .

save_


save_software_5
   _Saveframe_category   software

   _Name                 Olivia
   _Version              1.10.5

   loop_
      _Vendor
      _Address
      _Electronic_address

      'Yokochi, M.' . . 

   stop_

   loop_
      _Task

      'data analysis' 

   stop_

   _Details              .

save_


save_software_6
   _Saveframe_category   software

   _Name                 SPARKY
   _Version              3.110

   loop_
      _Vendor
      _Address
      _Electronic_address

      'Goddard, T.D.' . . 
      'Kneller, D.G.' . . 

   stop_

   loop_
      _Task

      'data analysis' 

   stop_

   _Details              .

save_


#########################
#  Experimental detail  #
#########################

    ##################################
    #  NMR Spectrometer definitions  #
    ##################################

save_spectrometer_1
   _Saveframe_category   NMR_spectrometer

   _Manufacturer         Bruker
   _Model                AVANCE
   _Field_strength       800
   _Details              .

save_


    #############################
    #  NMR applied experiments  #
    #############################

save_3D_15N-separated_NOESY_1
   _Saveframe_category   NMR_applied_experiment

   _Experiment_name     '3D 15N-separated NOESY'
   _Sample_label        $sample_1

save_


save_3D_HNCA_2
   _Saveframe_category   NMR_applied_experiment

   _Experiment_name     '3D HNCA'
   _Sample_label        $sample_1

save_


save_3D_HN(CO)CA_3
   _Saveframe_category   NMR_applied_experiment

   _Experiment_name     '3D HN(CO)CA'
   _Sample_label        $sample_1

save_


save_3D_15N-separated_NOESY
   _Saveframe_category                     NMR_applied_experiment

   _Experiment_name                       '3D 15N-separated NOESY'
   _BMRB_pulse_sequence_accession_number   .
   _Details                                .

save_


save_3D_HNCA
   _Saveframe_category                     NMR_applied_experiment

   _Experiment_name                       '3D HNCA'
   _BMRB_pulse_sequence_accession_number   .
   _Details                                .

save_


save_3D_HN(CO)CA
   _Saveframe_category                     NMR_applied_experiment

   _Experiment_name                       '3D HN(CO)CA'
   _BMRB_pulse_sequence_accession_number   .
   _Details                                .

save_


#######################
#  Sample conditions  #
#######################

save_condition_1
   _Saveframe_category   sample_conditions

   _Details              .

   loop_
      _Variable_type
      _Variable_value
      _Variable_value_error
      _Variable_value_units

      'ionic strength' 320   0.1   mM  
       pH                7.0 0.05  pH  
       pressure          1   0.001 atm 
       temperature     318   0.1   K   

   stop_

save_


####################
#  NMR parameters  #
####################

    ##############################
    #  Assigned chemical shifts  #
    ##############################

	################################
	#  Chemical shift referencing  #
	################################

save_reference_1
   _Saveframe_category   chemical_shift_reference

   _Details             
;
Chemical shift reference of 1H was based on the proton of water (4.784ppm at 
298K) and then those of 15N and 13C were calculated based on their gyromagnetic 
ratios. 
;

   loop_
      _Mol_common_name
      _Atom_type
      _Atom_isotope_number
      _Atom_group
      _Chem_shift_units
      _Chem_shift_value
      _Reference_method
      _Reference_type
      _External_reference_sample_geometry
      _External_reference_location
      _External_reference_axis
      _Indirect_shift_ratio

      DSS C 13 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 
      DSS H  1 'methyl protons' ppm 0.0 . indirect . . . 1.0         
      DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 

   stop_

save_


	###################################
	#  Assigned chemical shift lists  #
	###################################

###################################################################
#       Chemical Shift Ambiguity Index Value Definitions          #
#                                                                 #
# The values other than 1 are used for those atoms with different #
# chemical shifts that cannot be assigned to stereospecific atoms #
# or to specific residues or chains.                              #
#                                                                 #
#   Index Value            Definition                             #
#                                                                 #
#      1             Unique (including isolated methyl protons,   #
#                         geminal atoms, and geminal methyl       #
#                         groups with identical chemical shifts)  #
#                         (e.g. ILE HD11, HD12, HD13 protons)     #
#      2             Ambiguity of geminal atoms or geminal methyl #
#                         proton groups (e.g. ASP HB2 and HB3     #
#                         protons, LEU CD1 and CD2 carbons, or    #
#                         LEU HD11, HD12, HD13 and HD21, HD22,    #
#                         HD23 methyl protons)                    #
#      3             Aromatic atoms on opposite sides of          #
#                         symmetrical rings (e.g. TYR HE1 and HE2 #
#                         protons)                                #
#      4             Intraresidue ambiguities (e.g. LYS HG and    #
#                         HD protons or TRP HZ2 and HZ3 protons)  #
#      5             Interresidue ambiguities (LYS 12 vs. LYS 27) #
#      6             Intermolecular ambiguities (e.g. ASP 31 CA   #
#                         in monomer 1 and ASP 31 CA in monomer 2 #
#                         of an asymmetrical homodimer, duplex    #
#                         DNA assignments, or other assignments   #
#                         that may apply to atoms in one or more  #
#                         molecule in the molecular assembly)     #
#      9             Ambiguous, specific ambiguity not defined    #
#                                                                 #
###################################################################
save_chemical_shift_1
   _Saveframe_category               assigned_chemical_shifts

   _Details                          .

   loop_
      _Experiment_label

      '3D 15N-separated NOESY' 
      '3D HNCA'                
      '3D HN(CO)CA'            

   stop_

   loop_
      _Sample_label

      $sample_1 

   stop_

   _Sample_conditions_label         $condition_1
   _Chem_shift_reference_set_label  $reference_1
   _Mol_system_component_name        RimM
   _Text_data_format                 .
   _Text_data                        .

   loop_
      _Atom_shift_assign_ID
      _Residue_author_seq_code
      _Residue_seq_code
      _Residue_label
      _Atom_name
      _Atom_type
      _Chem_shift_value
      _Chem_shift_value_error
      _Chem_shift_ambiguity_code

        1   2   2 ARG CA C  56.462 0.300 1 
        2   3   3 LEU H  H   8.262 0.030 1 
        3   3   3 LEU CA C  53.391 0.300 1 
        4   3   3 LEU N  N 125.016 0.300 1 
        5   4   4 VAL H  H   9.095 0.030 1 
        6   4   4 VAL CA C  59.303 0.300 1 
        7   4   4 VAL N  N 118.528 0.300 1 
        8   5   5 GLU H  H   9.366 0.030 1 
        9   5   5 GLU CA C  57.345 0.300 1 
       10   5   5 GLU N  N 129.549 0.300 1 
       11   6   6 ILE CA C  61.104 0.300 1 
       12   7   7 GLY H  H   7.523 0.030 1 
       13   7   7 GLY CA C  46.924 0.300 1 
       14   7   7 GLY N  N 108.219 0.300 1 
       15   8   8 ARG H  H   8.335 0.030 1 
       16   8   8 ARG CA C  54.510 0.300 1 
       17   8   8 ARG N  N 119.201 0.300 1 
       18   9   9 PHE H  H   8.192 0.030 1 
       19   9   9 PHE CA C  58.573 0.300 1 
       20   9   9 PHE N  N 121.108 0.300 1 
       21  10  10 GLY H  H   9.127 0.030 1 
       22  10  10 GLY CA C  43.717 0.300 1 
       23  10  10 GLY N  N 114.078 0.300 1 
       24  11  11 ALA H  H   7.865 0.030 1 
       25  11  11 ALA CA C  50.972 0.300 1 
       26  11  11 ALA N  N 119.135 0.300 1 
       27  12  12 PRO CA C  62.489 0.300 1 
       28  13  13 TYR H  H   8.466 0.030 1 
       29  13  13 TYR CA C  56.406 0.300 1 
       30  13  13 TYR N  N 123.921 0.300 1 
       31  14  14 ALA H  H   8.423 0.030 1 
       32  14  14 ALA CA C  52.879 0.300 1 
       33  14  14 ALA N  N 129.547 0.300 1 
       34  15  15 LEU H  H   8.107 0.030 1 
       35  15  15 LEU CA C  56.217 0.300 1 
       36  15  15 LEU N  N 118.761 0.300 1 
       37  16  16 LYS H  H   8.445 0.030 1 
       38  16  16 LYS CA C  56.065 0.300 1 
       39  16  16 LYS N  N 120.625 0.300 1 
       40  17  17 GLY H  H   7.656 0.030 1 
       41  17  17 GLY CA C  44.952 0.300 1 
       42  17  17 GLY N  N 105.877 0.300 1 
       43  18  18 GLY H  H   8.018 0.030 1 
       44  18  18 GLY CA C  45.369 0.300 1 
       45  18  18 GLY N  N 108.219 0.300 1 
       46  19  19 LEU H  H   8.334 0.030 1 
       47  19  19 LEU CA C  54.207 0.300 1 
       48  19  19 LEU N  N 124.862 0.300 1 
       49  20  20 ARG H  H   7.612 0.030 1 
       50  20  20 ARG CA C  57.089 0.300 1 
       51  20  20 ARG N  N 120.172 0.300 1 
       52  21  21 PHE H  H   9.070 0.030 1 
       53  21  21 PHE CA C  57.582 0.300 1 
       54  21  21 PHE N  N 124.394 0.300 1 
       55  22  22 ARG H  H   7.783 0.030 1 
       56  22  22 ARG CA C  53.600 0.300 1 
       57  22  22 ARG N  N 129.079 0.300 1 
       58  23  23 GLY H  H   7.724 0.030 1 
       59  23  23 GLY CA C  45.900 0.300 1 
       60  23  23 GLY N  N 109.627 0.300 1 
       61  24  24 GLU H  H   8.364 0.030 1 
       62  24  24 GLU CA C  54.320 0.300 1 
       63  24  24 GLU N  N 120.399 0.300 1 
       64  25  25 PRO CA C  64.524 0.300 1 
       65  26  26 VAL H  H   6.895 0.030 1 
       66  26  26 VAL CA C  63.841 0.300 1 
       67  26  26 VAL N  N 117.400 0.300 1 
       68  27  27 VAL H  H   6.877 0.030 1 
       69  27  27 VAL CA C  63.575 0.300 1 
       70  27  27 VAL N  N 118.767 0.300 1 
       71  28  28 LEU H  H   7.250 0.030 1 
       72  28  28 LEU CA C  57.013 0.300 1 
       73  28  28 LEU N  N 118.064 0.300 1 
       74  29  29 HIS H  H   7.692 0.030 1 
       75  29  29 HIS CA C  55.307 0.300 1 
       76  29  29 HIS N  N 115.017 0.300 1 
       77  30  30 LEU H  H   7.127 0.030 1 
       78  30  30 LEU CA C  53.903 0.300 1 
       79  30  30 LEU N  N 118.767 0.300 1 
       80  31  31 GLU H  H   8.864 0.030 1 
       81  31  31 GLU CA C  56.444 0.300 1 
       82  31  31 GLU N  N 116.651 0.300 1 
       83  32  32 ARG H  H   7.801 0.030 1 
       84  32  32 ARG CA C  54.776 0.300 1 
       85  32  32 ARG N  N 116.656 0.300 1 
       86  33  33 VAL H  H   8.762 0.030 1 
       87  33  33 VAL CA C  59.100 0.300 1 
       88  33  33 VAL N  N 111.031 0.300 1 
       89  34  34 TYR H  H   9.074 0.030 1 
       90  34  34 TYR CA C  56.293 0.300 1 
       91  34  34 TYR N  N 125.071 0.300 1 
       92  35  35 VAL H  H   8.235 0.030 1 
       93  35  35 VAL CA C  61.641 0.300 1 
       94  35  35 VAL N  N 129.079 0.300 1 
       95  36  36 GLU H  H   8.752 0.030 1 
       96  36  36 GLU CA C  58.265 0.300 1 
       97  36  36 GLU N  N 130.017 0.300 1 
       98  37  37 GLY CA C  46.053 0.300 1 
       99  38  38 HIS H  H   8.319 0.030 1 
      100  38  38 HIS CA C  57.886 0.300 1 
      101  38  38 HIS N  N 118.310 0.300 1 
      102  39  39 GLY H  H   7.767 0.030 1 
      103  39  39 GLY CA C  43.472 0.300 1 
      104  39  39 GLY N  N 107.288 0.300 1 
      105  40  40 TRP H  H   8.176 0.030 1 
      106  40  40 TRP CA C  57.848 0.300 1 
      107  40  40 TRP N  N 120.172 0.300 1 
      108  41  41 ARG H  H   9.838 0.030 1 
      109  41  41 ARG CA C  53.562 0.300 1 
      110  41  41 ARG N  N 122.749 0.300 1 
      111  42  42 ALA H  H   8.489 0.030 1 
      112  42  42 ALA CA C  51.855 0.300 1 
      113  42  42 ALA N  N 124.393 0.300 1 
      114  43  43 ILE H  H   7.916 0.030 1 
      115  43  43 ILE CA C  62.020 0.300 1 
      116  43  43 ILE N  N 121.111 0.300 1 
      117  44  44 GLU H  H   9.073 0.030 1 
      118  44  44 GLU CA C  57.089 0.300 1 
      119  44  44 GLU N  N 128.846 0.300 1 
      120  45  45 ASP H  H   7.846 0.030 1 
      121  45  45 ASP CA C  53.751 0.300 1 
      122  45  45 ASP N  N 115.958 0.300 1 
      123  46  46 LEU H  H   8.376 0.030 1 
      124  46  46 LEU CA C  55.420 0.300 1 
      125  46  46 LEU N  N 123.219 0.300 1 
      126  47  47 TYR H  H   8.253 0.030 1 
      127  47  47 TYR CA C  56.065 0.300 1 
      128  47  47 TYR N  N 119.468 0.300 1 
      129  48  48 ARG H  H   8.481 0.030 1 
      130  48  48 ARG CA C  55.117 0.300 1 
      131  48  48 ARG N  N 118.765 0.300 1 
      132  49  49 VAL H  H   8.606 0.030 1 
      133  49  49 VAL CA C  61.868 0.300 1 
      134  49  49 VAL N  N 124.858 0.300 1 
      135  51  51 GLU CA C  56.520 0.300 1 
      136  52  52 GLU H  H   7.992 0.030 1 
      137  52  52 GLU CA C  55.913 0.300 1 
      138  52  52 GLU N  N 119.471 0.300 1 
      139  53  53 LEU H  H   8.644 0.030 1 
      140  53  53 LEU CA C  54.510 0.300 1 
      141  53  53 LEU N  N 123.458 0.300 1 
      142  54  54 VAL H  H   8.725 0.030 1 
      143  54  54 VAL CA C  61.337 0.300 1 
      144  54  54 VAL N  N 124.625 0.300 1 
      145  55  55 VAL H  H   8.828 0.030 1 
      146  55  55 VAL CA C  59.744 0.300 1 
      147  55  55 VAL N  N 122.749 0.300 1 
      148  56  56 HIS H  H   8.800 0.030 1 
      149  56  56 HIS CA C  54.510 0.300 1 
      150  56  56 HIS N  N 125.096 0.300 1 
      151  57  57 LEU H  H   9.630 0.030 1 
      152  57  57 LEU CA C  52.272 0.300 1 
      153  57  57 LEU N  N 124.861 0.300 1 
      154  58  58 ALA H  H   8.803 0.030 1 
      155  58  58 ALA CA C  53.751 0.300 1 
      156  58  58 ALA N  N 126.501 0.300 1 
      157  59  59 GLY H  H   8.987 0.030 1 
      158  59  59 GLY CA C  45.255 0.300 1 
      159  59  59 GLY N  N 109.627 0.300 1 
      160  60  60 VAL H  H   7.910 0.030 1 
      161  60  60 VAL CA C  62.324 0.300 1 
      162  60  60 VAL N  N 124.625 0.300 1 
      163  61  61 THR H  H   6.725 0.030 1 
      164  61  61 THR CA C  61.679 0.300 1 
      165  61  61 THR N  N 109.626 0.300 1 
      166  62  62 ASP H  H   7.412 0.030 1 
      167  62  62 ASP CA C  53.296 0.300 1 
      168  62  62 ASP N  N 115.484 0.300 1 
      169  63  63 ARG H  H   7.871 0.030 1 
      170  63  63 ARG CA C  58.910 0.300 1 
      171  63  63 ARG N  N 119.468 0.300 1 
      172  64  64 THR H  H   8.157 0.030 1 
      173  64  64 THR CA C  66.344 0.300 1 
      174  64  64 THR N  N 115.720 0.300 1 
      175  65  65 LEU H  H   8.231 0.030 1 
      176  65  65 LEU CA C  57.355 0.300 1 
      177  65  65 LEU N  N 122.749 0.300 1 
      178  66  66 ALA H  H   7.541 0.030 1 
      179  66  66 ALA CA C  55.079 0.300 1 
      180  66  66 ALA N  N 121.576 0.300 1 
      181  67  67 GLU H  H   8.282 0.030 1 
      182  67  67 GLU CA C  59.251 0.300 1 
      183  67  67 GLU N  N 116.657 0.300 1 
      184  68  68 ALA H  H   6.907 0.030 1 
      185  68  68 ALA CA C  53.827 0.300 1 
      186  68  68 ALA N  N 117.591 0.300 1 
      187  69  69 LEU H  H   7.754 0.030 1 
      188  69  69 LEU CA C  54.169 0.300 1 
      189  69  69 LEU N  N 117.347 0.300 1 
      190  70  70 VAL H  H   6.799 0.030 1 
      191  70  70 VAL CA C  65.092 0.300 1 
      192  70  70 VAL N  N 119.003 0.300 1 
      193  71  71 GLY H  H   7.914 0.030 1 
      194  71  71 GLY CA C  45.293 0.300 1 
      195  71  71 GLY N  N 114.540 0.300 1 
      196  72  72 LEU H  H   7.796 0.030 1 
      197  72  72 LEU CA C  55.003 0.300 1 
      198  72  72 LEU N  N 120.641 0.300 1 
      199  73  73 ARG H  H   8.926 0.030 1 
      200  73  73 ARG CA C  57.393 0.300 1 
      201  73  73 ARG N  N 118.995 0.300 1 
      202  74  74 VAL H  H   7.930 0.030 1 
      203  74  74 VAL CA C  61.072 0.300 1 
      204  74  74 VAL N  N 119.702 0.300 1 
      205  75  75 TYR H  H   9.486 0.030 1 
      206  75  75 TYR CA C  55.989 0.300 1 
      207  75  75 TYR N  N 124.164 0.300 1 
      208  76  76 ALA H  H   9.069 0.030 1 
      209  76  76 ALA CA C  49.617 0.300 1 
      210  76  76 ALA N  N 120.874 0.300 1 
      211  77  77 GLU H  H   8.611 0.030 1 
      212  77  77 GLU CA C  55.989 0.300 1 
      213  77  77 GLU N  N 122.986 0.300 1 
      214  78  78 VAL H  H   8.373 0.030 1 
      215  78  78 VAL CA C  66.420 0.300 1 
      216  78  78 VAL N  N 125.772 0.300 1 
      217  79  79 ALA H  H   9.238 0.030 1 
      218  79  79 ALA CA C  53.903 0.300 1 
      219  79  79 ALA N  N 121.339 0.300 1 
      220  80  80 ASP H  H   7.928 0.030 1 
      221  80  80 ASP CA C  54.548 0.300 1 
      222  80  80 ASP N  N 116.891 0.300 1 
      223  81  81 LEU H  H   7.246 0.030 1 
      224  81  81 LEU CA C  53.638 0.300 1 
      225  81  81 LEU N  N 119.186 0.300 1 
      226  83  83 PRO CA C  62.639 0.300 1 
      227  84  84 LEU H  H   8.289 0.030 1 
      228  84  84 LEU CA C  52.955 0.300 1 
      229  84  84 LEU N  N 122.524 0.300 1 
      230  85  85 GLU H  H   8.387 0.030 1 
      231  85  85 GLU CA C  56.141 0.300 1 
      232  85  85 GLU N  N 120.871 0.300 1 
      233  86  86 GLU H  H   8.099 0.030 1 
      234  86  86 GLU CA C  58.000 0.300 1 
      235  86  86 GLU N  N 120.408 0.300 1 
      236  87  87 GLY H  H   8.435 0.030 1 
      237  87  87 GLY CA C  45.103 0.300 1 
      238  87  87 GLY N  N 111.502 0.300 1 
      239  88  88 ARG H  H   7.450 0.030 1 
      240  88  88 ARG CA C  55.382 0.300 1 
      241  88  88 ARG N  N 118.532 0.300 1 
      242  89  89 TYR H  H   8.343 0.030 1 
      243  89  89 TYR CA C  56.255 0.300 1 
      244  89  89 TYR N  N 118.307 0.300 1 
      245  90  90 TYR H  H   8.468 0.030 1 
      246  90  90 TYR CA C  59.365 0.300 1 
      247  90  90 TYR N  N 121.343 0.300 1 
      248  91  91 TYR H  H   9.797 0.030 1 
      249  91  91 TYR N  N 124.389 0.300 1 
      250  92  92 PHE H  H   8.780 0.030 1 
      251  92  92 PHE CA C  59.972 0.300 1 
      252  92  92 PHE N  N 112.201 0.300 1 
      253  93  93 ALA H  H   6.744 0.030 1 
      254  93  93 ALA CA C  52.955 0.300 1 
      255  93  93 ALA N  N 122.281 0.300 1 
      256  94  94 LEU H  H   7.170 0.030 1 
      257  94  94 LEU CA C  54.586 0.300 1 
      258  94  94 LEU N  N 115.720 0.300 1 
      259  95  95 ILE H  H   7.252 0.030 1 
      260  95  95 ILE CA C  64.372 0.300 1 
      261  95  95 ILE N  N 119.012 0.300 1 
      262  96  96 GLY H  H   8.919 0.030 1 
      263  96  96 GLY CA C  44.231 0.300 1 
      264  96  96 GLY N  N 116.191 0.300 1 
      265  97  97 LEU H  H   7.654 0.030 1 
      266  97  97 LEU CA C  53.296 0.300 1 
      267  97  97 LEU N  N 120.385 0.300 1 
      268  98  98 PRO CA C  62.102 0.300 1 
      269  99  99 VAL H  H   7.744 0.030 1 
      270  99  99 VAL CA C  60.086 0.300 1 
      271  99  99 VAL N  N 118.990 0.300 1 
      272 100 100 TYR H  H   9.239 0.030 1 
      273 100 100 TYR CA C  56.786 0.300 1 
      274 100 100 TYR N  N 127.672 0.300 1 
      275 101 101 VAL H  H   8.671 0.030 1 
      276 101 101 VAL CA C  61.641 0.300 1 
      277 101 101 VAL N  N 119.719 0.300 1 
      278 102 102 GLU H  H   9.700 0.030 1 
      279 102 102 GLU CA C  57.506 0.300 1 
      280 102 102 GLU N  N 127.203 0.300 1 
      281 103 103 GLY H  H   8.830 0.030 1 
      282 103 103 GLY CA C  45.748 0.300 1 
      283 103 103 GLY N  N 103.298 0.300 1 
      284 104 104 ARG H  H   8.013 0.030 1 
      285 104 104 ARG CA C  54.207 0.300 1 
      286 104 104 ARG N  N 121.101 0.300 1 
      287 105 105 GLN H  H   9.011 0.030 1 
      288 105 105 GLN CA C  56.710 0.300 1 
      289 105 105 GLN N  N 126.015 0.300 1 
      290 106 106 VAL H  H   8.765 0.030 1 
      291 106 106 VAL CA C  60.731 0.300 1 
      292 106 106 VAL N  N 119.468 0.300 1 
      293 107 107 GLY H  H   7.383 0.030 1 
      294 107 107 GLY CA C  46.469 0.300 1 
      295 107 107 GLY N  N 107.283 0.300 1 
      296 108 108 GLU H  H   8.656 0.030 1 
      297 108 108 GLU CA C  54.510 0.300 1 
      298 108 108 GLU N  N 121.572 0.300 1 
      299 109 109 VAL H  H   8.600 0.030 1 
      300 109 109 VAL CA C  63.841 0.300 1 
      301 109 109 VAL N  N 123.452 0.300 1 
      302 110 110 VAL H  H   9.274 0.030 1 
      303 110 110 VAL CA C  61.489 0.300 1 
      304 110 110 VAL N  N 123.456 0.300 1 
      305 111 111 ASP H  H   8.111 0.030 1 
      306 111 111 ASP CA C  52.879 0.300 1 
      307 111 111 ASP N  N 118.734 0.300 1 
      308 112 112 ILE H  H   8.683 0.030 1 
      309 112 112 ILE CA C  60.693 0.300 1 
      310 112 112 ILE N  N 119.736 0.300 1 
      311 113 113 LEU H  H   9.270 0.030 1 
      312 113 113 LEU CA C  53.069 0.300 1 
      313 113 113 LEU N  N 127.452 0.300 1 
      314 114 114 ASP CA C  54.093 0.300 1 
      315 115 115 ALA H  H   8.291 0.030 1 
      316 115 115 ALA CA C  51.362 0.300 1 
      317 115 115 ALA N  N 131.189 0.300 1 
      318 116 116 GLY H  H   8.547 0.030 1 
      319 116 116 GLY CA C  47.000 0.300 1 
      320 116 116 GLY N  N 110.097 0.300 1 
      321 117 117 ALA H  H   7.843 0.030 1 
      322 117 117 ALA CA C  52.917 0.300 1 
      323 117 117 ALA N  N 124.393 0.300 1 
      324 118 118 GLN H  H   7.627 0.030 1 
      325 118 118 GLN CA C  53.751 0.300 1 
      326 118 118 GLN N  N 114.030 0.300 1 
      327 119 119 ASP H  H   9.371 0.030 1 
      328 119 119 ASP CA C  53.258 0.300 1 
      329 119 119 ASP N  N 120.409 0.300 1 
      330 120 120 VAL H  H   9.587 0.030 1 
      331 120 120 VAL CA C  61.679 0.300 1 
      332 120 120 VAL N  N 123.689 0.300 1 
      333 121 121 LEU CA C  55.307 0.300 1 
      334 122 122 ILE H  H   8.366 0.030 1 
      335 122 122 ILE CA C  61.451 0.300 1 
      336 122 122 ILE N  N 122.395 0.300 1 
      337 123 123 ILE H  H   9.279 0.030 1 
      338 123 123 ILE CA C  59.555 0.300 1 
      339 123 123 ILE N  N 128.607 0.300 1 
      340 124 124 ARG H  H   8.652 0.030 1 
      341 124 124 ARG CA C  54.738 0.300 1 
      342 124 124 ARG N  N 126.968 0.300 1 
      343 125 125 GLY H  H   8.606 0.030 1 
      344 125 125 GLY CA C  45.710 0.300 1 
      345 125 125 GLY N  N 112.546 0.300 1 
      346 126 126 VAL H  H   7.491 0.030 1 
      347 126 126 VAL CA C  60.844 0.300 1 
      348 126 126 VAL N  N 116.421 0.300 1 
      349 127 127 GLY H  H   8.261 0.030 1 
      350 127 127 GLY CA C  45.217 0.300 1 
      351 127 127 GLY N  N 111.037 0.300 1 
      352 128 128 GLU H  H   8.422 0.030 1 
      353 128 128 GLU CA C  57.620 0.300 1 
      354 128 128 GLU N  N 120.419 0.300 1 
      355 129 129 ARG H  H   8.325 0.030 1 
      356 129 129 ARG CA C  56.558 0.300 1 
      357 129 129 ARG N  N 119.233 0.300 1 
      358 130 130 LEU CA C  56.482 0.300 1 
      359 131 131 ARG H  H   8.099 0.030 1 
      360 131 131 ARG CA C  57.469 0.300 1 
      361 131 131 ARG N  N 118.395 0.300 1 
      362 132 132 ASP H  H   7.908 0.030 1 
      363 132 132 ASP CA C  54.965 0.300 1 
      364 132 132 ASP N  N 118.531 0.300 1 
      365 133 133 ARG H  H   7.811 0.030 1 
      366 133 133 ARG CA C  55.875 0.300 1 
      367 133 133 ARG N  N 118.765 0.300 1 
      368 134 134 ALA H  H   7.827 0.030 1 
      369 134 134 ALA CA C  52.576 0.300 1 
      370 134 134 ALA N  N 123.454 0.300 1 
      371 135 135 GLU H  H   8.374 0.030 1 
      372 135 135 GLU CA C  56.369 0.300 1 
      373 135 135 GLU N  N 120.174 0.300 1 
      374 136 136 ARG H  H   8.961 0.030 1 
      375 136 136 ARG CA C  53.713 0.300 1 
      376 136 136 ARG N  N 121.820 0.300 1 
      377 137 137 LEU H  H   8.256 0.030 1 
      378 137 137 LEU CA C  54.472 0.300 1 
      379 137 137 LEU N  N 123.218 0.300 1 
      380 139 139 PRO CA C  62.513 0.300 1 
      381 140 140 LEU H  H   9.328 0.030 1 
      382 140 140 LEU CA C  57.620 0.300 1 
      383 140 140 LEU N  N 126.740 0.300 1 
      384 141 141 GLN H  H   7.578 0.030 1 
      385 141 141 GLN CA C  59.251 0.300 1 
      386 141 141 GLN N  N 112.198 0.300 1 
      387 142 142 ALA H  H   6.717 0.030 1 
      388 142 142 ALA CA C  49.731 0.300 1 
      389 142 142 ALA N  N 121.579 0.300 1 
      390 143 143 PRO CA C  63.714 0.300 1 
      391 144 144 TYR H  H   5.758 0.030 1 
      392 144 144 TYR CA C  55.041 0.300 1 
      393 144 144 TYR N  N 111.032 0.300 1 
      394 145 145 VAL H  H   7.088 0.030 1 
      395 145 145 VAL CA C  61.906 0.300 1 
      396 145 145 VAL N  N 122.755 0.300 1 
      397 146 146 ARG H  H   9.367 0.030 1 
      398 146 146 ARG CA C  54.889 0.300 1 
      399 146 146 ARG N  N 127.558 0.300 1 
      400 147 147 VAL H  H   8.755 0.030 1 
      401 147 147 VAL CA C  62.589 0.300 1 
      402 147 147 VAL N  N 125.558 0.300 1 
      403 148 148 GLU H  H   8.652 0.030 1 
      404 148 148 GLU CA C  54.472 0.300 1 
      405 148 148 GLU N  N 127.905 0.300 1 
      406 149 149 GLU CA C  58.339 0.300 1 
      407 150 150 GLY H  H   8.576 0.030 1 
      408 150 150 GLY CA C  45.938 0.300 1 
      409 150 150 GLY N  N 111.270 0.300 1 
      410 151 151 SER H  H   7.545 0.030 1 
      411 151 151 SER CA C  58.569 0.300 1 
      412 151 151 SER N  N 110.568 0.300 1 
      413 152 152 ILE H  H   8.424 0.030 1 
      414 152 152 ILE CA C  59.365 0.300 1 
      415 152 152 ILE N  N 119.014 0.300 1 
      416 153 153 HIS H  H   9.297 0.030 1 
      417 153 153 HIS CA C  55.003 0.300 1 
      418 153 153 HIS N  N 127.205 0.300 1 
      419 154 154 VAL H  H   8.879 0.030 1 
      420 154 154 VAL CA C  59.896 0.300 1 
      421 154 154 VAL N  N 122.752 0.300 1 
      422 155 155 ASP H  H   8.909 0.030 1 
      423 155 155 ASP CA C  51.627 0.300 1 
      424 155 155 ASP N  N 130.012 0.300 1 
      425 156 156 PRO CA C  63.424 0.300 1 
      426 158 158 PRO CA C  64.903 0.300 1 
      427 159 159 GLY H  H   8.909 0.030 1 
      428 159 159 GLY CA C  45.369 0.300 1 
      429 159 159 GLY N  N 112.439 0.300 1 
      430 160 160 LEU H  H   7.700 0.030 1 
      431 160 160 LEU CA C  57.734 0.300 1 
      432 160 160 LEU N  N 120.881 0.300 1 
      433 161 161 PHE H  H   8.785 0.030 1 
      434 161 161 PHE CA C  55.989 0.300 1 
      435 161 161 PHE N  N 114.549 0.300 1 
      436 162 162 ASP H  H   7.351 0.030 1 
      437 162 162 ASP CA C  55.959 0.300 1 
      438 162 162 ASP N  N 125.333 0.300 1 

   stop_

save_