BMRB Entry 7434

Title:
Backbone assignment of 3F5 heavy chain antibody fragment in its free form and in complex with PABPN1
Deposition date:
2009-10-27
Original release date:
2009-11-24
Authors:
Impagliazzo, Antonietta; Ubbink, Marcellus
Citation:

Citation: Impagliazzo, Antonietta; Tepper, Armand; Verrips, Theo; Ubbink, Marcellus; van der Maarel, Silvere. "Structural insight into the recognition of PABPN1 by the flexible loops of a heavy chain antibody fragment"  .

Assembly members:

Assembly members:
3F5_VHH, polymer, 136 residues, Formula weight is not available
PABPN1pept, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Lama glama   Taxonomy ID: 9844   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lama glama

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28

Data sets:
Data typeCount
13C chemical shifts228
15N chemical shifts115
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
13F51
2PABPN1pept2

Entities:

Entity 1, 3F5 136 residues - Formula weight is not available

1   METALAGLUVALGLNLEUVALGLUSERGLY
2   GLYGLYLEUVALGLNALAGLYGLYSERLEU
3   ARGLEUSERCYSALAALASERGLYARGTHR
4   PHESERGLYTYRGLYMETGLYTRPPHEARG
5   GLNALAPROGLYLYSGLUARGGLUPHEVAL
6   ALAALAILESERTRPARGGLYGLYASNTHR
7   TYRTYRALAASPSERVALLYSGLYARGPHE
8   THRILESERARGASPASNALALYSASNTHR
9   VALTRPLEUGLNMETASNSERLEULYSPRO
10   GLUASPTHRALAVALTYRTYRCYSSERGLY
11   PHEVALARGTHRARGASPASPPROSERARG
12   ILEARGASNTYRTRPGLYGLNGLYTHRGLN
13   VALTHRVALSERTHRALAALAALALEUGLU
14   HISHISHISHISHISHIS

Entity 2, PABPN1pept 32 residues - Formula weight is not available

1   ALAILEGLUASPPROGLULEUGLUALAILE
2   LYSALAARGVALARGGLUMETGLUGLUGLU
3   ALAGLULYSLEULYSGLULEUGLNASNGLU
4   VALLYS

Samples:

sample_1: 3F5 VHH, [U-98% 15N], 1.6 mM; PABPN1 pept, [U-98% 15N], 1.6 mM; H2O 90%; D2O 10%; NaCl 150 mM

sample_2: 3F5 VHH, [U-98% 13C; U-98% 15N], 1.6 mM; PABPN1 pept, [U-98% 13C; U-98% 15N], 1.6 mM; H2O 90%; D2O 10%; NaCl 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 16586

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks