BMRB Entry 7434

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7434

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Title:
Backbone assignment of 3F5 heavy chain antibody fragment in its free form and in complex with PABPN1
Deposition date:
2009-10-27
Original release date:
2009-11-24
Authors:
Impagliazzo, Antonietta; Ubbink, Marcellus
Citation:

Citation: Impagliazzo, Antonietta; Tepper, Armand; Verrips, Theo; Ubbink, Marcellus; van der Maarel, Silvere. "Structural insight into the recognition of PABPN1 by the flexible loops of a heavy chain antibody fragment"  .

Assembly members:

Assembly members:
3F5_VHH, polymer, 136 residues, Formula weight is not available
PABPN1pept, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Lama glama   Taxonomy ID: 9844   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lama glama

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
3F5_VHH: MAEVQLVESGGGLVQAGGSL RLSCAASGRTFSGYGMGWFR QAPGKEREFVAAISWRGGNT YYADSVKGRFTISRDNAKNT VWLQMNSLKPEDTAVYYCSG FVRTRDDPSRIRNYWGQGTQ VTVSTAAALEHHHHHH
PABPN1pept: AIEDPELEAIKARVREMEEE AEKLKELQNEVK

Data sets:
Data typeCount
13C chemical shifts228
15N chemical shifts115
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
13F51
2PABPN1pept2

Entities:

Entity 1, 3F5 136 residues - Formula weight is not available

1   METALAGLUVALGLNLEUVALGLUSERGLY
2   GLYGLYLEUVALGLNALAGLYGLYSERLEU
3   ARGLEUSERCYSALAALASERGLYARGTHR
4   PHESERGLYTYRGLYMETGLYTRPPHEARG
5   GLNALAPROGLYLYSGLUARGGLUPHEVAL
6   ALAALAILESERTRPARGGLYGLYASNTHR
7   TYRTYRALAASPSERVALLYSGLYARGPHE
8   THRILESERARGASPASNALALYSASNTHR
9   VALTRPLEUGLNMETASNSERLEULYSPRO
10   GLUASPTHRALAVALTYRTYRCYSSERGLY
11   PHEVALARGTHRARGASPASPPROSERARG
12   ILEARGASNTYRTRPGLYGLNGLYTHRGLN
13   VALTHRVALSERTHRALAALAALALEUGLU
14   HISHISHISHISHISHIS

Entity 2, PABPN1pept 32 residues - Formula weight is not available

1   ALAILEGLUASPPROGLULEUGLUALAILE
2   LYSALAARGVALARGGLUMETGLUGLUGLU
3   ALAGLULYSLEULYSGLULEUGLNASNGLU
4   VALLYS

Related Database Links:

BMRB 16586

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks