BMRB Entry 7433

Title:
Extracellular CD147 (EMMPRIN): Isoform -3
Deposition date:
2009-05-28
Original release date:
2009-06-23
Authors:
Schelgel, Jennifer; Redzic, Jasmina; Porter, Christopher; Yurchenko, Vyacheslav; Bukrinsky, Michael; Armstrong, Geoffrey; Zhang, Fengli; Isern, Nancy; Degregori, James; Hodges, Robert; Eisenmesser, Elan
Citation:

Citation: Schlegel, Jennifer; Redzic, Jasmina; Porter, Christopher; Yurchenko, Vyacheslav; Bukrinsky, Michael; Labeikovsky, Wladimir; Armstrong, Geoffrey; Zhang, Fengli; Isern, Nancy; DeGregori, James; Hodges, Robert; Eisenmesser, Elan Zohar. "Solution characterization of the extracellular region of CD147 and its interaction with its enzyme ligand cyclophilin A."  J. Mol. Biol. 391, 518-535 (2009).
PubMed: 19500591

Assembly members:

Assembly members:
CD147_94-205, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts194
15N chemical shifts97
1H chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Extracellular CD147 Isoform-31

Entities:

Entity 1, Extracellular CD147 Isoform-3 125 residues - Formula weight is not available

1   GLYSERHISMETMETGLYTHRALAASNILE
2   GLNLEUHISGLYPROPROARGVALLYSALA
3   VALLYSSERSERGLUHISILEASNGLUGLY
4   GLUTHRALAMETLEUVALCYSLYSSERGLU
5   SERVALPROPROVALTHRASPTRPALATRP
6   TYRLYSILETHRASPSERGLUASPLYSALA
7   LEUMETASNGLYSERGLUSERARGPHEPHE
8   VALSERSERSERGLNGLYARGSERGLULEU
9   HISILEGLUASNLEUASNMETGLUALAASP
10   PROGLYGLNTYRARGCYSASNGLYTHRSER
11   SERLYSGLYSERASPGLNALAILEILETHR
12   LEUARGVALARGSERHISLEUALAALALEU
13   TRPPROPHELEUGLY

Samples:

sample_1: sodium phosphate 50 mM; sodium chloride 150 mM; imidazole 50 mM; CD147 94-205, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 93%

sample_conditions_1: ionic strength: 0.232 M; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian VNMRS 900 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 720 MHz

Related Database Links:

BMRB 16322
PDB
DBJ BAA08109 BAB88938 BAC67167 BAC67168 BAC76828
EMBL CAA45716 CAH92402
GB AAA68936 AAA91084 AAB41120 AAC33279 AAD10704
REF NP_001128860 NP_001719 NP_940991 NP_940992 NP_940993
SP P35613 Q865R3
AlphaFold Q865R3 P35613

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks