BMRB Entry 7428

Title:
Local and global structure of the monomeric subunit of the potassium channel KcsA probed by NMR
Deposition date:
2008-07-02
Original release date:
2008-08-07
Authors:
Chill, Jordan; Louis, John; Delaglio, Frank; Bax, Ad
Citation:

Citation: Chill, Jordan; Louis, John; Delaglio, Frank; Bax, Ad. "Local and global structure of the monomeric subunit of the potassium channel KcsA probed by NMR"  Biochim. Biophys. Acta. 1768, 3260-3270 (2007).
PubMed: 17945182

Assembly members:

Assembly members:
KcsA, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1902   Superkingdom: bacteria   Kingdom: not available   Genus/species: Streptomyces coelicolor

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
13C chemical shifts284
15N chemical shifts139
1H chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KcsA, 11
2KcsA, 21
3KcsA, 31
4KcsA, 41

Entities:

Entity 1, KcsA, 1 145 residues - Formula weight is not available

The first residue with number 1 is in reality the 16th residue.

1   LEULEUGLYARGHISGLYSERALALEUHIS
2   TRPARGALAALAGLYALAALATHRVALLEU
3   LEUVALILEVALLEULEUALAGLYSERTYR
4   LEUALAVALLEUALAGLUARGGLYALAPRO
5   GLYALAGLNLEUILETHRTYRPROARGALA
6   LEUTRPTRPSERVALGLUTHRALATHRTHR
7   VALGLYTYRGLYASPLEUTYRPROVALTHR
8   LEUTRPGLYARGLEUVALALAVALVALVAL
9   METVALALAGLYILETHRSERPHEGLYLEU
10   VALTHRALAALALEUALATHRTRPPHEVAL
11   GLYARGGLUGLNGLUARGARGGLYHISPHE
12   VALARGHISSERGLULYSALAALAGLUGLU
13   ALATYRTHRARGTHRTHRARGALALEUHIS
14   GLUARGPHEASPARGLEUGLUARGMETLEU
15   ASPASPASNARGARG

Samples:

sample_4_for_TableS5: KcsA 0.5-0.6 mM; MES 25 mM

sample_conditions_2: pH: 6; pressure: 14.1 Torr; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_4_for_TableS5isotropicsample_conditions_2
3D HN(CA)COsample_4_for_TableS5isotropicsample_conditions_2
3D HNCAsample_4_for_TableS5isotropicsample_conditions_2

Software:

NMRPipe/NMRDraw, NIH - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15853
PDB
EMBL CAA86025 CAC16993
GB AIJ11233 EFD64549 EOY52587
REF NP_631700 WP_003971485 WP_016328171 WP_030864493
SP P0A333 P0A334
AlphaFold P0A334 P0A333

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks