BMRB Entry 7422

Title:
Pseudo Contact shifts and RDCs of Galectin-3 with C-terminal LBT tagged at a proton frequency of 600MHz.
Deposition date:
2008-04-01
Original release date:
2008-06-30
Authors:
Zhuang, Tiandi; Lee, Han-seung; Imperiali, Barbara; Prestegard, James
Citation:

Citation: Zhuang, Tiandi; Lee, Han-seung; Imperiali, Barbara; Prestegard, James. "Structure determination of a Galectin-3-carbohydrate complex using paramagnetism-based NMR constraints"  Protein Sci. 17, 1220-1231 (2008).
PubMed: 18413860

Assembly members:

Assembly members:
Galectin-3-LBT, polymer, 155 residues, Formula weight is not available
LU, non-polymer, 174.967 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29-a

Data sets:
Data typeCount
1H chemical shifts34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Galectin-3-LBT1
2LUTETIUM ion2

Entities:

Entity 1, Galectin-3-LBT 155 residues - Formula weight is not available

1   METLEUILEVALPROTYRASNLEUPROLEU
2   PROGLYGLYVALVALPROARGMETLEUILE
3   THRILELEUGLYTHRVALLYSPROASNALA
4   ASNARGILEALALEUASPPHEGLNARGGLY
5   ASNASPVALALAPHEHISPHEASNPROARG
6   PHEASNGLUARGASNARGARGVALILEVAL
7   CYSASNTHRLYSLEUASPASNASNTRPGLY
8   ARGGLUGLUARGGLNSERVALPHEPROPHE
9   GLUSERGLYLYSPROPHELYSILEGLNVAL
10   LEUVALGLUPROASPHISPHELYSVALALA
11   VALASNASPALAHISLEULEUGLNTYRASN
12   HISARGVALLYSLYSLEUASNGLUILESER
13   LYSLEUGLYILESERGLYASPILEASPLEU
14   THRSERALASERTYRTHRMETILETYRILE
15   ASPTHRASNASNASPGLYTRPTYRGLUGLY
16   ASPGLULEULEUALA

Entity 2, LUTETIUM ion - Lu - 174.967 Da.

1   LU

Samples:

Diamagnetic_sample: Galectin-3-LBT, [U-15N], 0.3 mM; MES 50 mM; sodium chloride 50 mM; Lutetium chloride 0.3 mM

Diamagnetic_condition: ionic strength: 50 mM; pH: 5.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCDiamagnetic_sampleisotropicDiamagnetic_condition

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

VNMRJ, Varian - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15705 19491 4909
PDB
DBJ BAA22164 BAD92628 BAG37435 BAI46476
EMBL CAD61918 CAG33178 CAG46894
GB AAA35607 AAA36163 AAA88086 AAB26229 AAB86584
REF NP_002297 XP_001148424 XP_002824813 XP_003831735 XP_004055252
SP P17931
AlphaFold P17931