BMRB Entry 7406

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7406

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Title:
NMR resonance assignment of the Ccc2ab protein
Deposition date:
2007-09-06
Original release date:
2008-06-25
Authors:
Banci, Lucia; Bertini, Ivano; Chasapis, Christos; Rosato, Antonio; Tenroi, Leonardo
Citation:

Citation: Banci, L.; Bertini, I.; Chasapis, C.; Rosato, A.; Tenori, L.. "Interaction of the two soluble metal-binding domains of yeast Ccc2 with copper(I)-Atx1."  Biochem. Biophys. Res. Commun. 364, 645-649 (2007).
PubMed: 17961510

Assembly members:

Assembly members:
Ccc2, polymer, 150 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 20b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ccc2: MREVILAVHGMTCSACTNTI NTQLRALKGVTKCDISLVTN ECQVTYDNEVTADSIKEIIE DCGFDCEILRDSEITAISTK EGLLSVQGMTCGSCVSTVTK QVEGIEGVESVVVSLVTEEC HVIYEPSKTTLETAREMIED CGFDSNIIMD

Data sets:
Data typeCount
13C chemical shifts247
15N chemical shifts128
1H chemical shifts128

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ccc21
2CU12

Entities:

Entity 1, ccc2 150 residues - Formula weight is not available

1   METARGGLUVALILELEUALAVALHISGLY
2   METTHRCYSSERALACYSTHRASNTHRILE
3   ASNTHRGLNLEUARGALALEULYSGLYVAL
4   THRLYSCYSASPILESERLEUVALTHRASN
5   GLUCYSGLNVALTHRTYRASPASNGLUVAL
6   THRALAASPSERILELYSGLUILEILEGLU
7   ASPCYSGLYPHEASPCYSGLUILELEUARG
8   ASPSERGLUILETHRALAILESERTHRLYS
9   GLUGLYLEULEUSERVALGLNGLYMETTHR
10   CYSGLYSERCYSVALSERTHRVALTHRLYS
11   GLNVALGLUGLYILEGLUGLYVALGLUSER
12   VALVALVALSERLEUVALTHRGLUGLUCYS
13   HISVALILETYRGLUPROSERLYSTHRTHR
14   LEUGLUTHRALAARGGLUMETILEGLUASP
15   CYSGLYPHEASPSERASNILEILEMETASP

Entity 2, CU1 - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: Ccc2, [U-100% 13C; U-100% 15N], 0.2-0.5 mM; CU1 mM

sample_conditions_1: pH: 7.00; pressure: 1 atm; temperature: 298 K; viscosity: 100 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 15461

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks