BMRB Entry 7395

Title:
Solution Structure of RWD/GI domain of Saccharomyces cerevisiae GCN2
Deposition date:
2007-06-29
Original release date:
2015-06-16
Authors:
Ogura, K.; Torikai, S.; Kumeta, H.; Inagaki, F.
Citation:

Citation: Ogura, K.; Torikai, S.; Kumeta, H.; Inagaki, F.. "Solution structure of RWD/GI domain of Saccharomyces cerevisiae GCN2"  .

Assembly members:

Assembly members:
Serine-threonine-protein_kinase_GCN2, polymer, . residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts130
1H chemical shifts919

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RWD/GI domain1

Entities:

Entity 1, RWD/GI domain - Formula weight is not available

1   METSERLEUSERHISLEUTHRLEUASPGLN
2   TYRTYRGLUILEGLNCYSASNGLULEUGLU
3   ALAILEARGSERILETYRMETASPASPPHE
4   THRASPLEUTHRLYSARGLYSSERSERTRP
5   ASPLYSGLNPROGLNILEILEPHEGLUILE
6   THRLEUARGSERVALASPLYSGLUPROVAL
7   GLUSERSERILETHRLEUHISPHEALAMET
8   THRPROMETTYRPROTYRTHRALAPROGLU
9   ILEGLUPHELYSASNVALGLNASNVALMET
10   ASPSERGLNLEUGLNMETLEULYSSERGLU
11   PHELYSLYSILEHISASNTHRSERARGGLY
12   GLNGLUILEILEPHEGLUILETHRSERPHE
13   THRGLNGLULYSLEUASPGLUPHEGLNASN
14   VALVALASNTHRGLNSERLEUGLU

Samples:

sample: RWD/GI domain, [U-15N; U-13C], 1 mM; phosphate buffer 20 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 .; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESYsampleisotropicsample_conditions_1
3D_15N-separated_NOESYsampleisotropicsample_conditions_1

Software:

VNMR - collection

NMRPipe - processing

DYANA - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks