BMRB Entry 7359

Title:
1H, 15N and 13C chemical shift assignments for reduced and oxidised forms of the DsbA oxidoreductase from Vibrio cholerae
Deposition date:
2007-01-10
Original release date:
2007-06-26
Authors:
Horne, Henry; Scanlon, Martin
Citation:

Citation: Horne, James; Scanlon, Martin. "Backbone and side chain 1H, 15N and 13C assignments for the reduced form of the oxidoreductase protein DsbA from Vibrio cholerae"  Biomol. NMR Assignments 1, 75-76 (2007).
PubMed: 19636831

Assembly members:

Assembly members:
DsbA protein disulfide isomerase, polymer, 181 residues, 20443.3 Da.

Natural source:

Natural source:   Common Name: cholera   Taxonomy ID: 666   Superkingdom: Eukaryota   Kingdom: Not applicable   Genus/species: Vibrio cholerae

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pKc-type

Data sets:
Data typeCount
13C chemical shifts526
15N chemical shifts182
1H chemical shifts1157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DsbA1

Entities:

Entity 1, DsbA 181 residues - 20443.3 Da.

V148A cloning artefact in original clone gifted to this laboratory

1   ALAGLNPHELYSGLUGLYGLUHISTYRGLN
2   VALLEULYSTHRPROALASERSERSERPRO
3   VALVALSERGLUPHEPHESERPHETYRCYS
4   PROHISCYSASNTHRPHEGLUPROILEILE
5   ALAGLNLEULYSGLNGLNLEUPROGLUGLY
6   ALALYSPHEGLNLYSASNHISVALSERPHE
7   METGLYGLYASNMETGLYGLNALAMETSER
8   LYSALATYRALATHRMETILEALALEUGLU
9   VALGLUASPLYSMETVALPROVALMETPHE
10   ASNARGILEHISTHRLEUARGLYSPROPRO
11   LYSASPGLUGLNGLULEUARGGLNILEPHE
12   LEUASPGLUGLYILEASPALAALALYSPHE
13   ASPALAALATYRASNGLYPHEALAVALASP
14   SERMETVALARGARGPHEASPLYSGLNPHE
15   GLNASPSERGLYLEUTHRGLYVALPROALA
16   VALVALVALASNASNARGTYRLEUVALGLN
17   GLYGLNSERALALYSSERLEUASPGLUTYR
18   PHEASPLEUVALASNTYRLEULEUTHRLEU
19   LYS

Samples:

sample-DsbA-oxidised: DsbA - Bacterial oxidoreductase, [U-95% 13C; U-90% 15N], 0.3 mM; HEPES 10 mM; NaCl 50 mM

conditions_1: pH: 6.8; temperature: 300 K

conditions_2: pH: 6.8; temperature: 320 K

Experiments:

NameSampleSample stateSample conditions
13C_1H-HSQCsample-DsbA-oxidisednot availablenot available
15N_1H-HSQCsample-DsbA-oxidisednot availablenot available
CBCACONNHsample-DsbA-oxidisednot availablenot available
HNCACBsample-DsbA-oxidisednot availablenot available
HNCAsample-DsbA-oxidisednot availablenot available
HNCOsample-DsbA-oxidisednot availablenot available
HNCACOsample-DsbA-oxidisednot availablenot available
CCONHTOCSYsample-DsbA-oxidisednot availablenot available
HCCONHTOCSYsample-DsbA-oxidisednot availablenot available
HCCH-TOCSYsample-DsbA-oxidisednot availablenot available
13C-NOESY-HSQCsample-DsbA-oxidisednot availablenot available

Software:

SPARKY, UCSF - data visualisation

NMRPipe, NIH - data processing

NMR spectrometers:

  • VARIAN AVANCE 600 MHz
  • BRUKER DRX 500 MHz

Related Database Links:

BMRB 7360
PDB
DBJ BAP04237
EMBL CAA45977
GB AAA27568 AAF93212 ABQ20372 ACP04371 ACP08173
REF NP_229693 WP_000732279 WP_000732281 WP_000732282 WP_000732283
SP P32557
AlphaFold P32557

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks