BMRB Entry 7352

Name: SOLUTION STRUCTURE OF THE ZN COMPLEX OF EIAV NCP11(22-58) PEPTIDE, INCLUDING TWO CCHC ZN-BINDING MOTIFS.
Published: 2007-06-06

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PDB ID: 2bl6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7352
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF THE ZN COMPLEX OF EIAV NCP11(22-58) PEPTIDE, INCLUDING TWO CCHC ZN-BINDING MOTIFS.

Deposition date:

2006-12-01

Original release date:

2007-06-06

Authors:

Amodeo, P.; Castiglione-Morelli, M.; Ostuni, A.; Bavoso, A.

Citation:

Citation: Amodeo, P.; Castiglione-Morelli, M.; Ostuni, A.; Battistuzzi, G.; Bavoso, A.. "Structural Features in Eiav Ncp11: A Lentivirus Nucleocapsid Protein with a Short Linker" Biochemistry 45, 5517-5526 (2006).
PubMed: 16634633

Assembly members:

Assembly members:
GAG_POLYPROTEIN_-_NUCLEOCAPSID_PROTEIN_P11, polymer, 37 residues, 65.409 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: HIV-2 Taxonomy ID: 11709 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-2

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GAG_POLYPROTEIN_-_NUCLEOCAPSID_PROTEIN_P11: QTCYNCGKPGHLSSQCRAPK VCFKCKQPGHFSKQCRS

Data sets:

assigned_chemical_shifts
- chemical_shift_ch_A

Data type	Count
1H chemical shifts	255

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GAG POLYPROTEIN	1
2	ZINC ION, 1	2
3	ZINC ION, 2	2

Entities:

Entity 1, GAG POLYPROTEIN 37 residues - 65.409 Da.

1

GLN

THR

CYS

TYR

ASN

CYS

GLY

LYS

PRO

GLY

2

HIS

LEU

SER

GLN

CYS

ARG

ALA

PRO

LYS

3

VAL

CYS

PHE

LYS

CYS

LYS

GLN

PRO

GLY

HIS

4

PHE

SER

LYS

GLN

CYS

ARG

SER

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample: NCp8 2.5 mM; H2O 90%; D2O 10%; TCEP.HCl 37.5 mM; zinc chloride 7.5 mM

sample_conditions_1: ionic strength: 210 mM; pH: 5.8; pressure: 1.0 atm; temperature: 293.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample	isotropic	sample_conditions_1
TOCSY	sample	isotropic	sample_conditions_1

Software:

AMBER, CASE ET AL - refinement, structure solution

Molmol v2K.2 - data analysis

VNMR v6.1C - processing

NMR spectrometers:

VARIAN UNITYINOVA 500 MHz

PDB	2BL6
DBJ	BAB12103 BAB12109
GB	AAA43003 AAA43011 AAA43013 AAB59861 AAC03760
REF	NP_056901
SP	P69730 P69731 P69732
AlphaFold	P69730 P69731 P69732